The utility model provides a double-emulsification high-flux micro-fluidic
chip. The double-emulsification high-flux micro-fluidic
chip comprises a substrate, a middle layer and a cover plate, according to the utility model, the substrate is not processed, the main micro-channels are processed on the two sides of the middle layer, and the cover plate is punched, so that fluid can be conveniently introduced. A plurality of
liquid drop generation units based on the flow focusing technology are designed on one side of the middle layer so as to generate liquid drops for the first time, and a stepped
liquid drop generation
structure based on interfacial tension is designed on the other side of the middle layer so as to generate liquid drops for the second time. The structures on the two sides of the
chip are connected through the through holes. The preparation method comprises the following steps: carrying out photoetching to form a mold, preparing the middle layer by using the mold, forming the required micro-channels on the two sides of the middle layer,
punching on the cover plate, and finally bonding the substrate, the middle layer and the cover plate. Through the design of the flow channel and the structure, a large number of double-
emulsion droplets can be rapidly prepared, the production efficiency is improved, and the double-
emulsion droplet preparation device is suitable for various applications such as
drug delivery and biological research.