14 results about "Monodisperse droplets" patented technology

The precision extrusion forming device comprises a pressure PID controller, a pressure protector, an extrusion pipe valve, a slurry cavity, a spray head, a spray disc and a collecting disc, the pressure PID controller and the pressure protector are in electric control connection with the extrusion pipe valve, the extrusion pipe valve is connected with the upper end of the slurry cavity, the spray head is connected with the upper end of the slurry cavity, and the collecting disc is connected with the lower end of the slurry cavity. The spray head is connected with the lower end of the slurry cavity, the spray disc is annular, an air nozzle is arranged on the inner wall of the spray disc, the spray disc is located below an outlet of the spray head, and the liquid slurry is ejected out of the spray head under the driving of the extrusion force and forms monodisperse liquid drops under the action of airflow of the spray disc. The collecting disc is arranged below the spraying disc, a cooling medium is contained in the collecting disc and used for cooling and solidifying falling monodisperse liquid drops, and the protective gas is communicated with the slurry cavity. By adopting the forming device disclosed by the invention, the tritium breeding microspheres with uniform particle size, excellent performance and less lithium loss can be obtained.

The invention discloses gallium ion imprinted amidoxime-based macroporous resin as well as a preparation method and application thereof, and belongs to the technical field of functional polymer materials and separation. The method comprises the following steps: pre-assembling a gallium trichloride template and monomers such as acrylonitrile, generating monodisperse liquid drops by utilizing a microfluidic technology, polymerizing by combining ultraviolet light, eluting the template by hydrochloric acid, and performing amidoximation modification by a hydroxylamine hydrochloride alkaline solution, thereby obtaining the resin. The resin is a monodisperse spherical microsphere, has a macroporous structure, has high selective recognition capability and high adsorption capacity on gallium ions, is fast in adsorption kinetics and good in regeneration performance, and is suitable for selectively separating or enriching gallium ions from a complex solution system.

The present invention discloses a multi-compartment calcium alginate gel microcarrier, a preparation method thereof and an application thereof. In this method, multiple droplets of Dex-Alg solution are dropped into a biphasic receiving solution. After the Dex-Alg solution enters the lower phase and stands still, a multi-compartment calcium alginate gel microcarrier is obtained. The upper phase of the biphasic receiving solution is a PEG solution, and the lower phase is a Dex-CaCl2 solution. Multiple monodisperse droplets of Dex-Alg fuse at the liquid-liquid interface of the PEG solution and the Dex-CaCl2 solution in the biphasic receiving solution. After entering the lower phase, Alg crosslinks with Ca 2+ to form a multi-compartment calcium alginate gel microcarrier. The method of the present invention is easy to operate, can control the shape of the gel microcarrier by controlling the fusion of droplets, does not require the addition of organic reagents, has high biocompatibility, and is green and environmentally friendly; the controllable multi-compartment calcium alginate gel microcarrier prepared by the method of the present invention has broad application prospects.

This invention discloses a "cross"-shaped four-way parallel microfluidic emulsification system and its application, addressing the problems of difficult maintenance and poor product consistency during parallel scale-up of existing microfluidic emulsification technologies. The system includes a fluid drive and supply module, a fluid distribution module, and an emulsification module composed of multiple independent, detachable "cross"-shaped four-way microfluidic emulsification units connected in parallel. The key feature of each emulsification unit is that the microchannel diameters at the dispersed phase inlet and emulsion outlet are equal (dD=dE), and both are smaller than the microchannel diameter at the continuous phase inlet (dC). This design, combining modular structure and hierarchical flow distribution, significantly improves unit processing consistency, system maintainability, and flow uniformity during parallel scale-up, making it suitable for high-throughput preparation of monodisperse droplets and functional microspheres.

The invention belongs to the technical field of droplet jet printing, and relates to a multi-component droplet jet printing system and method. The device comprises a micro-fluidic base shell, a liquid drop generation module, a liquid drop detection module and a liquid drop sorting module, liquid in a plurality of parallel liquid channels is subjected to micro-fluidic mixing treatment through a micro-fluidic mixer, uniform mixed flow is obtained, the uniform mixed flow is treated through a continuous jet type micro-liquid drop generation method, and the liquid drop separation efficiency is improved. The continuous monodisperse liquid drops can be obtained, the continuous monodisperse liquid drops are detected through the liquid drop detection module, the continuous monodisperse liquid drops are processed through the liquid drop sorting module according to the detection result, non-printing target liquid drops can be subjected to directional deflection, and liquid drop sorting is completed. Through micro-fluidic mixing treatment, the component concentration proportion of the monodisperse liquid drops can be regulated and controlled online in real time, sorting and printing are carried out according to needs, and the device is suitable for the fields of pattern printing, electronic circuit printing, functional material screening, gradient biochemical reaction, additive manufacturing, biological manufacturing and the like.

The invention belongs to the field of preparation of monodisperse liquid drops of a high-viscosity system, and provides a method for preparing monodisperse liquid drops of a high-viscosity system through in-situ concentration, which comprises the following steps: (1) dissolving internal reagents including a high polymer material and a cross-linking agent in water to form an internal phase fluid, the viscosity of which does not exceed 100mpa. S; dissolving an oil-soluble surfactant in a concentrating agent to form an external phase fluid or a collection liquid; and (2) preparing the internal phase fluid and the external phase fluid into a monodisperse water-in-oil emulsion through a microfluidic device, collecting the water-in-oil emulsion by adopting a collecting solution, and carrying out in-situ concentration on the water-in-oil emulsion in the process of moving in the microfluidic device and keeping in the collecting solution to obtain the monodisperse liquid drops of a high-viscosity system. According to the method, the monodispersity of the high-viscosity system liquid drops prepared based on the microfluidic device can be effectively improved, and then the monodispersity and biocompatibility of the cross-linked polymer microspheres prepared by taking the obtained liquid drops as the template can be improved.

The invention discloses a preparation method of an airway organ, which comprises the following steps: dissociating and digesting airway tissues into single cells, and performing 2D multiplication culture to P3-P4 generations; uniformly mixing the cells subjected to 2D multiplication culture to P3-P4 generations with matrigel to obtain a cell-matrigel mixture; shearing the cell-matrigel mixture into monodisperse liquid drops by using a microfluidic technology, and curing the monodisperse liquid drops at 37 DEG C to obtain cell microspheres after curing; and inoculating the cell microspheres into an airway organoid culture medium, and culturing in a 5% CO2 incubator at 37 DEG C to obtain the airway organoid after the culture is finished. According to the method for constructing the airway organoid, the period is shortened, cell components such as airway epithelial cells and cilia cells are provided, and high-efficiency construction of the airway organoid can be achieved.

This invention discloses a microcarrier for delivering active mitochondria, its preparation method, and its applications. The microcarrier comprises porous hydrogel microspheres, engineered mitochondria encapsulated within the microspheres, and a polydopamine coating on its surface. The engineered mitochondria are obtained by surface modification of active mitochondria with a polyamide-amine dendritic polymer modified with phenylboronic acid. The preparation method involves mixing the engineered mitochondria with a hydrogel prepolymer as the inner phase, preparing water-in-oil monodisperse droplets using microfluidic technology, curing them under ultraviolet light to obtain hydrogel microspheres, and then mixing them with a dopamine solution to form a polydopamine coating. This microcarrier effectively maintains mitochondrial activity and promotes cellular uptake, repairs damaged mitochondrial function, resists oxidative stress, and promotes cell proliferation, migration, and angiogenesis. Animal experiments show that this microcarrier significantly accelerates the healing of photodamaged skin wounds. This invention can be used to prepare drugs for treating skin wounds and has promising clinical applications.

The invention relates to the technical field of polymers, and provides polyetherketoneketone powder based on a microfluidic technology and a preparation method and device thereof, and the method comprises the following steps: taking a first liquid containing polyetherketoneketone as a dispersion phase, and taking a second liquid as a continuous phase; the solvent of the first liquid is a first solvent; the boiling point of the first solvent is higher than the melting point of the polyether ketone ketone; the second liquid and the first solvent are immiscible; the dispersed phase with the temperature of T1 and the continuous phase with the temperature of T2 enter the micro-fluidic chip, the dispersed phase is located in an inner phase channel of the micro-fluidic chip, the continuous phase is located in an outer phase channel of the micro-fluidic chip, the outer phase channel is concentrically arranged outside the inner phase channel in a sleeving mode, and the continuous phase is located in an inner phase channel of the micro-fluidic chip. The dispersed phase is sheared by the continuous phase to form monodispersed droplets. By means of the method, the high sphericity degree and the regular morphology of the powder can be guaranteed. The particle size and distribution of the powder can be accurately controlled by adjusting parameters such as two-phase flow velocity, viscosity, chip size and the like.

The invention relates to the technical field of fluid mechanics, in particular to a droplet target generating device for an EUV light source, which comprises a liquid supply assembly, a droplet spraying assembly and a piezoelectric driving assembly, positive pressure is applied to the solution in the liquid storage device through the air pressure controller, the solution is supplied to the nozzle until the jet flow is extruded, then the negative pressure is switched, the jet flow is forced to stop, and the nozzle opening is kept on an inward meniscus; then, the piezoelectric element is driven through the driving signal controller, positive and negative alternating sound pressure waves are generated in the liquid in the capillary tube through deformation of the piezoelectric element, and the sound pressure waves are transmitted to the nozzle opening so as to eject liquid drops; moreover, by setting parameters of the driving signal controller, the device can generate stable liquid drops with different frequencies, is simple to operate, accurate to control and large in adjustable range, can generate stable large-spacing monodisperse liquid drops, and is suitable for generation of liquid drop targets in an EUV light source.

The invention provides devices that generate monodisperse droplets from a bulk liquid. The devices include a shearing mechanism, a holder for a vessel containing a liquid, and an optical system that transmit light to, and detects light from, liquid in the vessel. The invention also provides methods of using such devices to produce monodisperse droplets.

The invention belongs to the technical field of aerosol and fluid dynamics, and relates to a monodisperse fluorescent tracer micro-droplet-based instrument performance quantitative calibration method, which comprises the following steps of: marking a monodisperse droplet by using a fluorescent tracer agent; collecting and obtaining the total input amount of the marked monodisperse liquid drops entering the to-be-calibrated collecting device; extracting solutions containing fluorescent tracers and corresponding to the specific surfaces of the collecting device are obtained; and determining the collection efficiency and the inter-stage loss rate of each stage of the to-be-calibrated collection device. According to the invention, a standardized strict performance quantitative calibration method is provided for a collection device, especially a multistage fluid collection and separation device, by combining monodisperse fluorescence tracing micro-droplets with a standardized calibration process, so that the accuracy and consistency of core indexes such as capture efficiency, particle size separation precision and the like of the device are effectively guaranteed; the situation that the performance evaluation of the collecting device lacks a unified calibration standard is avoided, and meanwhile, real and accurate actual measurement data including the collecting efficiency of each stage and the inter-stage loss rate can be output.

This invention relates to a microfluidic monodisperse droplet generation device, method, and amplification reaction system, comprising a droplet generation component and a liquid storage tube. The droplet generation component has a liquid interface connected to the bottom end of the vertically positioned liquid storage tube. The liquid interface is connected to one end of a main liquid channel. The main liquid channel has multiple branch channels with a gradually expanding structure, the width of which gradually increases along the liquid flow direction. One end of each branch channel is connected to a liquid storage chamber, and the other end of the liquid storage chamber has a discharge channel. The height of the internal space of the liquid storage chamber is higher than the height of the branch channels, and the branch channels are connected to the lower part of the end of the liquid storage channel, so that a stepped structure is formed between the branch channels and the internal space of the liquid storage chamber. The generation device of this invention has a simple structure, low cost, and high portability.