47 results about "Monodisperse droplets" patented technology

A method for forming monodisperse lipoplex assemblies includes providing a microfluidic device having a main microfluidic channel coupled to first and second reactant inlet channels. The first inlet channel is used to deliver a cationic lipid to the main channel while the second channel is used to deliver a nucleic acid. A droplet generation zone is provided in the main channel at the intersection of first and second carrier channels that contain a hydrophobic fluid. The cationic lipid, nucleic acid, and the hydrophobic fluid are then pumped through the device. In the droplet generation zone, shear force from the hydrophobic fluid pinches off droplets. The cationic lipid and nucleic acid are mixed in the generated droplets as the droplets move within a mixing region. A plurality of splitting channels may be coupled to the outlet of the device to produce smaller, monodisperse droplets having picoliter volumes.

A high-throughput micro-droplet immobilization method, based on the principle of buoyancy and surface tension, is controlled on a microfluidic chip. The micro-droplet immobilization method specifically includes: injecting the continuous phase and the dispersed phase into the chip using a syringe pump, As a result, continuous monodisperse microdroplets are formed in the T-shaped microdroplet generation zone; the microdroplets continue to flow and enter the droplet catcher array driven by the syringe pump, and the droplets flow through the channel below the catcher due to buoyancy and The combined effect of surface tension enters the cylindrical catcher upwards; subsequent droplets flow through the lower channel of the droplet catcher that has successfully immobilized the droplet, and are sequentially captured in subsequent droplet catchers. The invention simplifies the principle and process of liquid droplet immobilization, the microfluidic chip involved is simple to manufacture, convenient to operate in experiments, stable in immobilization effect, does not cause damage to organisms in the liquid droplet, and is easy to carry out with other functional units on the chip. Flexible combination and scale integration.

The invention discloses a single / multiple-component droplet preparation device based on integrated micro-channels and a control method of the single / multiple-component droplet preparation device. The device comprises a micro-channel plastic film, a dispersed phase inlet joint, a dispersed phase outlet joint, a continuous phase container, an injection pump and the like, wherein one end of the micro-channel plastic film is connected with the dispersed phase inlet joint, the other end is connected with the dispersed phase outlet joint, the dispersed phase inlet joint is connected with the injection pump, and the dispersed phase outlet joint is immersed in the container filled with a continuous phase. A dispersed phase is pushed by the injection pump to flow and the flow is controlled, so that dispersed phase droplets can be generated in the continuous phase container. Monodispersed droplets can be generated rapidly with a large flux, multiple-component droplets can be prepared, and the device is expected to be applied to the fields of drug preparation, chemical synthesis, biomedicine and the like.

A method of creating substantially monodisperse droplets comprising supplying a first fluid (1) and a second immiscible fluid (2) within a set of channels, the second fluid surrounding the first fluid and filling the channels to form a composite jet. The composite jet passes through an entrance channel (4) into a wider cavity (3), where the first fluid breaks into droplets (5), the resulting composite of droplets of the first fluid within the second fluid passing through an exit channel (6). The cross sectional area of the exit channel perpendicular to the flow is smaller than the cross sectional area of the cavity and the passage of a droplet of the first fluid out of the cavity via the exit perturbs the composite flow field within the cavity such that the incoming jet of the first fluid is perturbed.

The methods described herein, referred to as particle-templated emulsification (PTE), provide an improved approach for generating a monodisperse emulsion that encapsulates target particles of interest without requiring the use of a microfluidic device. Monodisperse droplets may be effectively obtained by using monodisperse particles to template the formation of droplets, which can include, e.g., monodisperse single-emulsion droplets, multiple-emulsion droplets, or Giant Unilamellar Vesicles (GUV), without destroying the integrity of the droplets.

A method of creating substantially monodisperse droplets comprising supplying a first fluid (1) and a second immiscible fluid (2) within a set of channels, the second fluid surrounding the first fluid and filling the channels to form a composite jet. The composite jet passes through an entrance channel (4) into a wider cavity (3), where the first fluid breaks into droplets (5), the resulting composite of droplets of the first fluid within the second fluid passing through an exit channel (6). The cross sectional area of the exit channel perpendicular to the flow is smaller than the cross sectional area of the cavity and the passage of a droplet of the first fluid out of the cavity via the exit perturbs the composite flow field within the cavity such that the incoming jet of the first fluid is perturbed.

The invention concerns a method and apparatus (100) for producing mist of a liquid phase having very fine and mono-dispersed droplets. The method is realized by an apparatus (100) including a partition (101), defined by a first side surface (107) and a second side surface (113). The first side surface (107) is wetted by a liquid phase to form a film thereon, while the second side surface (113) is substantially dry. A gas stream is directed through the partition (101) from the dry side (113) to the wetted side (107) thereby forming a mist having droplets of less than 1 micron in size and a concentration of at least 1,000,000,000,000 per cubic cm.

The invention relates to a micro-fluid chip for decelerating fluid like droplets, cells and the like on the basis of the dielectrophoresis effect, and belongs to the technical field of micro-fluid chips. The micro-fluid chip comprises a substrate and a body structure arranged on and bonded with the substrate. The body structure comprises a micro-fluid pipeline for fluid circulating. An electrode I and an electrode II which are oppositely arranged are arranged outside the micro-fluid pipeline and on the substrate. The sides, close to the micro-fluid pipeline, of the electrode I and the electrode II are each in a square wave shape, and the other sides are each in a continuous belt shape. The micro-fluid chip has the advantages that the electrodes with special effects are added outside an existing micro-fluid conveying pipeline, due to the design of the electrodes, the aim of controlling the movements of droplets, cells and the like is achieved, more importantly, the electrodes are arranged on the two sides of a micro-fluid channel and do not extend into the micro-fluid channel, and through an active control method, damage-free control over single-dispersed droplets, biomolecules and molecules is achieved.

The invention provides a phase change energy storage fiber with isolation cavities. The matrix of the fiber is compact boric acid crosslinked polyvinyl alcohol. The matrix is provided with a plurality of isolation cavities which are isolated from each other, the cavities are distributed along the length of the fiber, and each cavity contains an oil soluble phase change material. The preparation method comprises the following steps: (1) preparing middle phase fluid, external phase fluid and receiving liquid; (2) by taking the oil phase change material as an internal phase fluid, respectively injecting the internal phase fluid, the middle phase fluid and the external phase fluid into an injection pipe, a transition pipe and a collecting pipe, enabling the internal phase fluid to enter the transition pipe by virtue of the injection pipe, shearing the internal phase fluid in the transition pipe into mono-dispersed drops by the middle phase fluid, enabling the middle phase fluid which carries the mono-dispersed drops to enter the collecting pipe through the transition pipe, and in the collecting pipe, carrying out crosslink curing on the components in the middle phase fluid and dehydrating to form fibers which are collected by using a receiving container for holding the receiving liquid; and (3) after the fibers in the receiving liquid are fully cured, taking out the fibers, and washing and drying to the fibers.

A method of introducing a sample into an atomic spectrometer utilizes a spray head including a vibratable mesh. A liquid sample is conducted to one face of the mesh and the mesh is vibrated to expel sample droplets from the other face of the mesh into the proximal end of a flow passage axially spaced from the mesh. Also, a low pressure gas as flowed into the proximal end of the flow passage to mix with the droplets to form an aerosol in the flow passage. The vibrating of the mesh is controlled to provide in the aerosol a selected total volume of monodisperse droplets while the flow of the carrier gas is independently controlled to provide a selected rate of flow of the aerosol along the flow passage thereby to optimize consumption of the sample. Apparatus for practicing the method is also disclosed.

Devices and methods for dividing droplets are generally described. In some embodiments, an article may comprise a fluidic channel comprising an array of obstructions. In certain embodiments, the arrangement of obstructions in the array may affect the flow path of fluid in the channel. For example, the array of obstructions may be used to convert a polydisperse population of droplets into a relatively monodisperse population of droplets. Passing a polydisperse population of droplets through the array may result in the division of droplets such that the population of droplets exiting the array has a narrower distribution in the characteristic dimensions of the droplets. The arrangement of obstructions in the array may allow for high-throughput production of a substantially monodisperse population of droplets in some cases. In some embodiments, the population of droplets exiting the array may be converted into particles.

The invention belongs to the technical field of fluid micro-dispersion and particularly relates to a micro-structural device for preparing mono-dispersed liquid drops and bubbles and a use method of the micro-structural device. The micro-structural device comprises a substrate, a cover plate and a capillary tube, wherein a step-shaped groove is formed in the substrate; the capillary tube is embedded into the upper half part of the groove, and dispersed phase liquid / air enters a main channel through the capillary tube; a continuous phase is in contact with a dispersed phase at a reducing position of the step structure, and the dispersed phase is sheared into the mono-dispersed bubbles and liquid drops. The micro-structural device is simple in structure, convenient to produce, less prone to blockage and large in operation range, and is not influenced by wettability of the channel; by setting parameters such as two-phase flow, the mono-dispersed liquid drops and bubbles of required sizes can be rapidly and accurately acquired; the micro-structural device is particularly applicable to the preparation of the mono-dispersed liquid drops and bubbles of 1-1000 microns and has good application prospects in fundamental research and practical industry.

The present invention provides a biological scaffold and a preparation method and an application thereof. The biological scaffold comprises a biological matrix and long-chain DNA entangled in the biological matrix. The biological scaffold is of a pore structure. The biological scaffold of the present invention adopts the biological matrix as the main scaffold material, and the long-chain DNA entangled in the biological matrix as a mechanical lock for locking biological matrix fiber. Sol containing a large amount of monodisperse droplets is rapidly gelated under the action of a shear force, soas to shorten gelation time of natural biological matrix and increase gelation speed by 10-100 times. The pore structure promotes the exchange of substances in the biological matrix, provides oxygen and nutrients to cells, discharges metabolic wastes, and improves survival rate of cell transplantation.

A method of introducing a sample into an atomic spectrometer utilizes a spray head including a vibratable mesh. A liquid sample is conducted to one face of the mesh and the mesh is vibrated to expel sample droplets from the other face of the mesh into the proximal end of a flow passage axially spaced from the mesh. Also, a low pressure gas as flowed into the proximal end of the flow passage to mix with the droplets to form an aerosol in the flow passage. The vibrating of the mesh is controlled to provide in the aerosol a selected total volume of monodisperse droplets while the flow of the carrier gas is independently controlled to provide a selected rate of flow of the aerosol along the flow passage thereby to optimize consumption of the sample. Apparatus for practicing the method is also disclosed.

The wet treatment of wafer-shaped articles is improved by utilizing a droplet generator designed to produce a spray of monodisperse droplets. The droplet generator is mounted above a spin chuck, and is moved across a major surface of the wafer-shaped article in a linear or arcuate path. The droplet generator includes a transducer acoustically coupled to its body such that sonic energy reaches a region of the body surrounding the discharge orifices. Each orifice has a width w of at least 1 μm and at most 200 μm and a height h such that a ratio of h to w is not greater than 1.

The invention discloses a double-flow monodisperse droplet flow generation method which comprises the following steps that: an injection pump pushes an injector to continuously inject liquid in a liquid storage bottle into a droplet flow generator, and the liquid is injected into air from two micropores of the droplet flow generator to form two jets; and a piezoelectric actuator sends voltage disturbance waves to a piezoelectric ceramic piece in the droplet flow generator, a droplet flow generator is driven to generate vibration with a certain frequency, and disturbance waves are formed on thetwo jets and broken into two beams of monodisperse droplet flows. The invention also discloses a double-flow monodisperse droplet flow generation device. The device comprises a droplet flow generator, an injector, an injection pump, a piezoelectric actuator and a liquid storage bottle, the droplet flow generator comprises a piezoelectric ceramic piece, and the piezoelectric actuator is connectedwith the piezoelectric ceramic piece. The double-flow monodisperse droplet flow generation method and device provided by the invention can solve the control problem of multi-beam droplet jet flow, andrealize droplet flow generation of different droplet particle sizes, droplet intervals and flow beam intervals.

The invention belongs to the technical field of multi-phase fluid micro-dispersion, and particularly relates to a microstructure device adopting series amplification. The microstructure device comprises a micro-channel substrate, a main channel and bypass channels, wherein the main channel is arranged on the micro-channel substrate, and the bypass channels are arranged on one or two sides of the main channel and are perpendicular to the main channel; capillary tubes are embedded in the bypass channels and throats are formed at junctions of the capillary tubes and the main channel; a continuous-phase fluid inlet tube is arranged at an upstream inlet of the main channel and a two-phase fluid outlet tube is arranged at a downstream outlet. The formed microstructure device adopting series amplification realizes scale amplification of the droplet and bubble breaking process under the jetting flow pattern and is simple in structure, convenient to manufacture and not easy to block, the mono-dispersed droplet and bubble generation frequency is substantially increased, and the microstructure device can be well applied to gas-liquid and liquid-liquid systems.

The invention includes a method and apparatus (100) for producing mist of a liquid phase having very fine and mono-dispersed droplets. The method is realized by an apparatus (100) including a partition (101), defined by a first side surface (107) and a second side surface (113). The first side surface (107) is wetted by a liquid phase to form a film thereon, while the second side surface (113) is substantially dry. A gas stream is directed through the partition (101) from the dry side (113) to the wetted side (107) thereby forming a mist having droplets of less than i micron in size and a concentration of at least 1,000,000,000,000 per cubic cm.

The invention belongs to the field of fluid mechanics, and particularly discloses a piezoelectric monodisperse continuous liquid drop generator. The piezoelectric monodisperse continuous liquid drop generator comprises a liquid storage tank, a spray head, piezoelectric ceramic, an electrifying ring and a deflection electric field, wherein the liquid storage tank is used for supplying liquid to the spray head; the piezoelectric ceramic is arranged above the spray head and is used for disturbing liquid in the spray head, so that the spray head generates uniform monodisperse liquid drops; the electrifying ring is arranged below the spray head and is used for electrifying part of liquid drops flowing out of the spray head; and the deflection electric field is arranged below the electrifying ring and is used for deflecting the charged liquid drops. According to the piezoelectric monodisperse continuous liquid drop generator, external disturbance is applied to liquid through the piezoelectric ceramic, so that liquid jet is regularly split to form uniform monodisperse liquid drop flow, meanwhile, the electrifying ring and the deflection electric field are arranged in a matched manner so that the liquid drops can be partially electrified and deflected, the number density of the liquid drops in a liquid drop string is reduced, and the distance between the liquid drops is adjusted.

The wet treatment of wafer-shaped articles is improved by utilizing a droplet generator designed to produce a spray of monodisperse droplets. The droplet generator is mounted above a spin chuck, and is moved across a major surface of the wafer-shaped article in a linear or arcuate path. The droplet generator includes a transducer acoustically coupled to its body such that sonic energy reaches a region of the body surrounding the discharge orifices. Each orifice has a width w of at least 1 μm and at most 200 μm and a height h such that a ratio of h to w is not greater than 1.

The invention discloses application of honeycomb-shaped GelMA microspheres in construction of a tumor model. The honeycomb-shaped GelMA microspheres take an isopropyl myristate solution as a continuous phase and a GelMA solution containing 1% of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methyl propiophenone as a dispersed phase, and a honeycomb-shaped structure is formed by freeze-dried GelMA microspheres through preparing monodisperse liquid drops with the same size in batches by adopting a microfluidic technology under a constant speed difference, exposing the liquid drops to 6.9mW / cm<2> ultraviolet light for photo-crosslinking, and carrying out cleaning. According to the invention, honeycomb-like porous GelMA hydrogel microspheres are formed by the microfluidic technology and are applied to three-dimensional culture of osteosarcoma cells. In-vivo and in-vitro experiments show that three-dimensional culture based on the honeycomb-shaped porous microspheres can maintain dryness of the osteosarcoma cells, biological characteristics of the osteosarcoma cells are better maintained, and a real in-vivo environment of the tumor cells can be better simulated.

The invention relates to a parthenocissus tricuspidata-imitated adhesion microcarrier and a preparation method thereof. Monodisperse droplets prepared by a microfluidic device are self-assembled in arapid solution extractant to form micron particles with parthenocissus tricuspidata morphology, the micron particles are used as a template, functional hydrogel with good biocompatibility is used forcopying the structure of the micron particles, and the template is removed to obtain the parthenocissus tricuspidata-imitated adhesion microcarrier. The microcarrier has a bionic macroscopic morphology, the contact area is increased, the adhesion effect is enhanced, the porous microstructure provides a large area for drug fixation, and a good carrier is provided for prolonging the drug release time through long-time retention of the drug in a complex environment through adhesion. The preparation method provided by the invention is simple and easy to implement, low in cost and convenient for large-scale production, and the prepared microcarrier is non-toxic and has excellent biocompatibility, biological functionality and biodegradability. And a new carrier is provided for safe and efficientdrug delivery.

The invention discloses a continuous crystallization micro-nano chemical chip and application thereof. The micro-nano chemical chip comprises a substrate and a cover plate covering the substrate. The substrate is provided with a micro-mixing part for forming a supersaturated mixed stock solution; a micro-dispersion part which is communicated with the micro-mixing part, and is internally filled with a flowing continuous phase, wherein the continuous phase and the supersaturated mixed stock solution are converged at the micro-dispersion part, and the continuous phase generates acting force on the supersaturated mixed stock solution, so that the supersaturated mixed stock solution is sheared into monodispersion liquid drops; and a continuous crystallization part which has a preset length, and is used for providing a place for continuous crystallization of monodisperse liquid drops to form monodisperse liquid drops wrapping crystals, wherein a liquid film exists between the monodisperse liquid drops and the inner wall of the continuous crystallization part, and the liquid drops are not infiltrated with the inner wall of the continuous crystallization part. According to the micro-nano chemical chip, continuous crystallization can be realized, uniform and stable crystallization conditions are provided, and the situation that crystals are attached and deposited on the wall surfaces of the channels to block the channels is avoided.

The invention belongs to the field of droplet fusion, and particularly relates to a method for initiating different monodisperse droplet fusion by utilizing liquid wettability. The method comprises the processing steps of: (1) preparing disperse water phase, medium oil phase and continuous phase liquid; (2) injecting each disperse phase liquid into a micro-fluidic device to form water-in-oil emulsion droplets and oil-pocket water-in-oil emulsion droplets, enabling the formed water-in-oil emulsion droplets and the oil-pocket water-in-oil emulsion droplets to enter a collecting tube along with the continuous phase fluid; paving oil layers at the surfaces of the oil-pocket water-in-oil emulsion droplets at the surfaces of the water-in-oil emulsion droplets when the water-in-oil emulsion droplets contact the oil-pocket water-in-oil emulsion droplets in an expanded chamber of the collecting tube; and discharging the oil layers between water drops in the oil-pocket water-in-oil emulsion droplets and the water drops in the water-in-oil emulsion droplets, wherein the water drops in the two droplets are fused to achieve micro-mixing or micro-reaction; and (3) collecting the fused droplets.

The invention discloses a preparation method of lignin microspheres prepared via mercapto group functionalization. The preparation method comprises following steps: 1, a dispersion phase solution is prepared; 2, a continuous phase solution is prepared; 3, water-in-oil lignin droplets are prepared; and 4, obtained lignin monodisperse droplets are heated so as to obtain the lignin microspheres via synthesis, wherein the discharge end of a focused-flow microchannel reactor is connected with a collection vessel, the lignin thiamine compound mixed droplets obtained in step 3 are collected, curing reaction is carried out at 55 to 95 DEG C for 2 to 12h so as to obtain the lignin microspheres prepared via mercapto group functionalization. Adsorbing capacity of the lignin microspheres prepared via mercapto group functionalization on cadmium ions in waste water is as high as 70.3mg / g, and is 8 times of that of alkali lignin; compared with common lignin adsorbents, the lignin microspheres prepared via mercapto group functionalization possess following advantages: particle size is uniform, morphology is controllable, mechanical properties are excellent, and the lignin microspheres are capable of tolerating water impact.

The invention relates to a method and an apparatus for generating substantially monodisperse droplets. The invention involves flowing a continuous phase fluid in a microfluidic passageway which extends along a longitudinal length direction and has a substantially constant cross section along the length direction. A dispersed phase fluid is introduced into the microfluidic passageway along a traverse direction through inlet orifices to generate droplets of the dispersed phase fluid in the continuous phase fluid. The inlet orifices have a substantially uniform diameter, and any adjacent two of the inlet orifices are arranged to be offset from each other in the length direction. The dispersed phase fluid is broken up by the shear stress generated by the continuous phase fluid to produce monodisperse droplets. By the offsetting arrangement of the inlet orifices, droplets formed by adjacent inlet orifices will not interfere with each other, thereby ensuring the uniformity of the droplets.

The invention belongs to the technical field of fluid micro-dispersion and particularly relates to a micro-structural device for preparing mono-dispersed liquid drops and bubbles and a use method of the micro-structural device. The micro-structural device comprises a substrate, a cover plate and a capillary tube, wherein a step-shaped groove is formed in the substrate; the capillary tube is embedded into the upper half part of the groove, and dispersed phase liquid / air enters a main channel through the capillary tube; a continuous phase is in contact with a dispersed phase at a reducing position of the step structure, and the dispersed phase is sheared into the mono-dispersed bubbles and liquid drops. The micro-structural device is simple in structure, convenient to produce, less prone to blockage and large in operation range, and is not influenced by wettability of the channel; by setting parameters such as two-phase flow, the mono-dispersed liquid drops and bubbles of required sizes can be rapidly and accurately acquired; the micro-structural device is particularly applicable to the preparation of the mono-dispersed liquid drops and bubbles of 1-1000 microns and has good application prospects in fundamental research and practical industry.

The invention relates to a micro-fluid chip for decelerating fluid like droplets, cells and the like on the basis of the dielectrophoresis effect, and belongs to the technical field of micro-fluid chips. The micro-fluid chip comprises a substrate and a body structure arranged on and bonded with the substrate. The body structure comprises a micro-fluid pipeline for fluid circulating. An electrode I and an electrode II which are oppositely arranged are arranged outside the micro-fluid pipeline and on the substrate. The sides, close to the micro-fluid pipeline, of the electrode I and the electrode II are each in a square wave shape, and the other sides are each in a continuous belt shape. The micro-fluid chip has the advantages that the electrodes with special effects are added outside an existing micro-fluid conveying pipeline, due to the design of the electrodes, the aim of controlling the movements of droplets, cells and the like is achieved, more importantly, the electrodes are arranged on the two sides of a micro-fluid channel and do not extend into the micro-fluid channel, and through an active control method, damage-free control over single-dispersed droplets, biomolecules and molecules is achieved.