5 results about "Monodisperse droplets" patented technology

This invention discloses a "cross"-shaped four-way parallel microfluidic emulsification system and its application, addressing the problems of difficult maintenance and poor product consistency during parallel scale-up of existing microfluidic emulsification technologies. The system includes a fluid drive and supply module, a fluid distribution module, and an emulsification module composed of multiple independent, detachable "cross"-shaped four-way microfluidic emulsification units connected in parallel. The key feature of each emulsification unit is that the microchannel diameters at the dispersed phase inlet and emulsion outlet are equal (dD=dE), and both are smaller than the microchannel diameter at the continuous phase inlet (dC). This design, combining modular structure and hierarchical flow distribution, significantly improves unit processing consistency, system maintainability, and flow uniformity during parallel scale-up, making it suitable for high-throughput preparation of monodisperse droplets and functional microspheres.

The present application belongs to the technical field of aerosol and fluid dynamics, and relates to an instrument performance quantitative calibration method based on monodisperse fluorescent tracer microdroplets, comprising the following steps: using monodisperse droplets labeled with a fluorescent tracer; collecting and obtaining the total amount of labeled monodisperse droplets input into a to-be-calibrated collection device; obtaining extraction liquid containing the fluorescent tracer corresponding to each specific surface of the collection device; and determining the collection efficiency and inter-stage loss rate of each stage of the to-be-calibrated collection device. The present application provides a standardized and strict performance quantitative calibration method for the collection device, especially a multi-stage fluid collection and separation device, by using monodisperse fluorescent tracer microdroplets in combination with a standardized calibration process, effectively guarantees the accuracy and consistency of core indicators such as device capture efficiency and particle size separation precision, avoids the lack of unified calibration standards for performance evaluation of the collection device, and can also output real and accurate measured data including the collection efficiency and inter-stage loss rate of each stage.

This invention discloses a microcarrier for delivering active mitochondria, its preparation method, and its applications. The microcarrier comprises porous hydrogel microspheres, engineered mitochondria encapsulated within the microspheres, and a polydopamine coating on its surface. The engineered mitochondria are obtained by surface modification of active mitochondria with a polyamide-amine dendritic polymer modified with phenylboronic acid. The preparation method involves mixing the engineered mitochondria with a hydrogel prepolymer as the inner phase, preparing water-in-oil monodisperse droplets using microfluidic technology, curing them under ultraviolet light to obtain hydrogel microspheres, and then mixing them with a dopamine solution to form a polydopamine coating. This microcarrier effectively maintains mitochondrial activity and promotes cellular uptake, repairs damaged mitochondrial function, resists oxidative stress, and promotes cell proliferation, migration, and angiogenesis. Animal experiments show that this microcarrier significantly accelerates the healing of photodamaged skin wounds. This invention can be used to prepare drugs for treating skin wounds and has promising clinical applications.

The invention provides devices that generate monodisperse droplets from a bulk liquid. The devices include a shearing mechanism, a holder for a vessel containing a liquid, and an optical system that transmit light to, and detects light from, liquid in the vessel. The invention also provides methods of using such devices to produce monodisperse droplets.

This invention relates to a microfluidic monodisperse droplet generation device, method, and amplification reaction system, comprising a droplet generation component and a liquid storage tube. The droplet generation component has a liquid interface connected to the bottom end of the vertically positioned liquid storage tube. The liquid interface is connected to one end of a main liquid channel. The main liquid channel has multiple branch channels with a gradually expanding structure, the width of which gradually increases along the liquid flow direction. One end of each branch channel is connected to a liquid storage chamber, and the other end of the liquid storage chamber has a discharge channel. The height of the internal space of the liquid storage chamber is higher than the height of the branch channels, and the branch channels are connected to the lower part of the end of the liquid storage channel, so that a stepped structure is formed between the branch channels and the internal space of the liquid storage chamber. The generation device of this invention has a simple structure, low cost, and high portability.