47 results about "Monodisperse droplets" patented technology

The invention discloses a single / multiple-component droplet preparation device based on integrated micro-channels and a control method of the single / multiple-component droplet preparation device. The device comprises a micro-channel plastic film, a dispersed phase inlet joint, a dispersed phase outlet joint, a continuous phase container, an injection pump and the like, wherein one end of the micro-channel plastic film is connected with the dispersed phase inlet joint, the other end is connected with the dispersed phase outlet joint, the dispersed phase inlet joint is connected with the injection pump, and the dispersed phase outlet joint is immersed in the container filled with a continuous phase. A dispersed phase is pushed by the injection pump to flow and the flow is controlled, so that dispersed phase droplets can be generated in the continuous phase container. Monodispersed droplets can be generated rapidly with a large flux, multiple-component droplets can be prepared, and the device is expected to be applied to the fields of drug preparation, chemical synthesis, biomedicine and the like.

A method of creating substantially monodisperse droplets comprising supplying a first fluid (1) and a second immiscible fluid (2) within a set of channels, the second fluid surrounding the first fluid and filling the channels to form a composite jet. The composite jet passes through an entrance channel (4) into a wider cavity (3), where the first fluid breaks into droplets (5), the resulting composite of droplets of the first fluid within the second fluid passing through an exit channel (6). The cross sectional area of the exit channel perpendicular to the flow is smaller than the cross sectional area of the cavity and the passage of a droplet of the first fluid out of the cavity via the exit perturbs the composite flow field within the cavity such that the incoming jet of the first fluid is perturbed.

The methods described herein, referred to as particle-templated emulsification (PTE), provide an improved approach for generating a monodisperse emulsion that encapsulates target particles of interest without requiring the use of a microfluidic device. Monodisperse droplets may be effectively obtained by using monodisperse particles to template the formation of droplets, which can include, e.g., monodisperse single-emulsion droplets, multiple-emulsion droplets, or Giant Unilamellar Vesicles (GUV), without destroying the integrity of the droplets.

A method of creating substantially monodisperse droplets comprising supplying a first fluid (1) and a second immiscible fluid (2) within a set of channels, the second fluid surrounding the first fluid and filling the channels to form a composite jet. The composite jet passes through an entrance channel (4) into a wider cavity (3), where the first fluid breaks into droplets (5), the resulting composite of droplets of the first fluid within the second fluid passing through an exit channel (6). The cross sectional area of the exit channel perpendicular to the flow is smaller than the cross sectional area of the cavity and the passage of a droplet of the first fluid out of the cavity via the exit perturbs the composite flow field within the cavity such that the incoming jet of the first fluid is perturbed.

The invention concerns a method and apparatus (100) for producing mist of a liquid phase having very fine and mono-dispersed droplets. The method is realized by an apparatus (100) including a partition (101), defined by a first side surface (107) and a second side surface (113). The first side surface (107) is wetted by a liquid phase to form a film thereon, while the second side surface (113) is substantially dry. A gas stream is directed through the partition (101) from the dry side (113) to the wetted side (107) thereby forming a mist having droplets of less than 1 micron in size and a concentration of at least 1,000,000,000,000 per cubic cm.

A method of introducing a sample into an atomic spectrometer utilizes a spray head including a vibratable mesh. A liquid sample is conducted to one face of the mesh and the mesh is vibrated to expel sample droplets from the other face of the mesh into the proximal end of a flow passage axially spaced from the mesh. Also, a low pressure gas as flowed into the proximal end of the flow passage to mix with the droplets to form an aerosol in the flow passage. The vibrating of the mesh is controlled to provide in the aerosol a selected total volume of monodisperse droplets while the flow of the carrier gas is independently controlled to provide a selected rate of flow of the aerosol along the flow passage thereby to optimize consumption of the sample. Apparatus for practicing the method is also disclosed.

The present invention provides a biological scaffold and a preparation method and an application thereof. The biological scaffold comprises a biological matrix and long-chain DNA entangled in the biological matrix. The biological scaffold is of a pore structure. The biological scaffold of the present invention adopts the biological matrix as the main scaffold material, and the long-chain DNA entangled in the biological matrix as a mechanical lock for locking biological matrix fiber. Sol containing a large amount of monodisperse droplets is rapidly gelated under the action of a shear force, soas to shorten gelation time of natural biological matrix and increase gelation speed by 10-100 times. The pore structure promotes the exchange of substances in the biological matrix, provides oxygen and nutrients to cells, discharges metabolic wastes, and improves survival rate of cell transplantation.

A method of introducing a sample into an atomic spectrometer utilizes a spray head including a vibratable mesh. A liquid sample is conducted to one face of the mesh and the mesh is vibrated to expel sample droplets from the other face of the mesh into the proximal end of a flow passage axially spaced from the mesh. Also, a low pressure gas as flowed into the proximal end of the flow passage to mix with the droplets to form an aerosol in the flow passage. The vibrating of the mesh is controlled to provide in the aerosol a selected total volume of monodisperse droplets while the flow of the carrier gas is independently controlled to provide a selected rate of flow of the aerosol along the flow passage thereby to optimize consumption of the sample. Apparatus for practicing the method is also disclosed.

The wet treatment of wafer-shaped articles is improved by utilizing a droplet generator designed to produce a spray of monodisperse droplets. The droplet generator is mounted above a spin chuck, and is moved across a major surface of the wafer-shaped article in a linear or arcuate path. The droplet generator includes a transducer acoustically coupled to its body such that sonic energy reaches a region of the body surrounding the discharge orifices. Each orifice has a width w of at least 1 μm and at most 200 μm and a height h such that a ratio of h to w is not greater than 1.

The invention discloses a double-flow monodisperse droplet flow generation method which comprises the following steps that: an injection pump pushes an injector to continuously inject liquid in a liquid storage bottle into a droplet flow generator, and the liquid is injected into air from two micropores of the droplet flow generator to form two jets; and a piezoelectric actuator sends voltage disturbance waves to a piezoelectric ceramic piece in the droplet flow generator, a droplet flow generator is driven to generate vibration with a certain frequency, and disturbance waves are formed on thetwo jets and broken into two beams of monodisperse droplet flows. The invention also discloses a double-flow monodisperse droplet flow generation device. The device comprises a droplet flow generator, an injector, an injection pump, a piezoelectric actuator and a liquid storage bottle, the droplet flow generator comprises a piezoelectric ceramic piece, and the piezoelectric actuator is connectedwith the piezoelectric ceramic piece. The double-flow monodisperse droplet flow generation method and device provided by the invention can solve the control problem of multi-beam droplet jet flow, andrealize droplet flow generation of different droplet particle sizes, droplet intervals and flow beam intervals.

The invention belongs to the field of fluid mechanics, and particularly discloses a piezoelectric monodisperse continuous liquid drop generator. The piezoelectric monodisperse continuous liquid drop generator comprises a liquid storage tank, a spray head, piezoelectric ceramic, an electrifying ring and a deflection electric field, wherein the liquid storage tank is used for supplying liquid to the spray head; the piezoelectric ceramic is arranged above the spray head and is used for disturbing liquid in the spray head, so that the spray head generates uniform monodisperse liquid drops; the electrifying ring is arranged below the spray head and is used for electrifying part of liquid drops flowing out of the spray head; and the deflection electric field is arranged below the electrifying ring and is used for deflecting the charged liquid drops. According to the piezoelectric monodisperse continuous liquid drop generator, external disturbance is applied to liquid through the piezoelectric ceramic, so that liquid jet is regularly split to form uniform monodisperse liquid drop flow, meanwhile, the electrifying ring and the deflection electric field are arranged in a matched manner so that the liquid drops can be partially electrified and deflected, the number density of the liquid drops in a liquid drop string is reduced, and the distance between the liquid drops is adjusted.

The invention discloses a single / multiple-component droplet preparation device based on integrated micro-channels and a control method of the single / multiple-component droplet preparation device. The device comprises a micro-channel plastic film, a dispersed phase inlet joint, a dispersed phase outlet joint, a continuous phase container, an injection pump and the like, wherein one end of the micro-channel plastic film is connected with the dispersed phase inlet joint, the other end is connected with the dispersed phase outlet joint, the dispersed phase inlet joint is connected with the injection pump, and the dispersed phase outlet joint is immersed in the container filled with a continuous phase. A dispersed phase is pushed by the injection pump to flow and the flow is controlled, so that dispersed phase droplets can be generated in the continuous phase container. Monodispersed droplets can be generated rapidly with a large flux, multiple-component droplets can be prepared, and the device is expected to be applied to the fields of drug preparation, chemical synthesis, biomedicine and the like.

A method for forming monodisperse lipoplex assemblies includes providing a microfluidic device having a main microfluidic channel coupled to first and second reactant inlet channels. The first inlet channel is used to deliver a cationic lipid to the main channel while the second channel is used to deliver a nucleic acid. A droplet generation zone is provided in the main channel at the intersection of first and second carrier channels that contain a hydrophobic fluid. The cationic lipid, nucleic acid, and the hydrophobic fluid are then pumped through the device. In the droplet generation zone, shear force from the hydrophobic fluid pinches off droplets. The cationic lipid and nucleic acid are mixed in the generated droplets as the droplets move within a mixing region. A plurality of splitting channels may be coupled to the outlet of the device to produce smaller, monodisperse droplets having picoliter volumes.

The invention discloses a continuous crystallization micro-nano chemical chip and application thereof. The micro-nano chemical chip comprises a substrate and a cover plate covering the substrate. The substrate is provided with a micro-mixing part for forming a supersaturated mixed stock solution; a micro-dispersion part which is communicated with the micro-mixing part, and is internally filled with a flowing continuous phase, wherein the continuous phase and the supersaturated mixed stock solution are converged at the micro-dispersion part, and the continuous phase generates acting force on the supersaturated mixed stock solution, so that the supersaturated mixed stock solution is sheared into monodispersion liquid drops; and a continuous crystallization part which has a preset length, and is used for providing a place for continuous crystallization of monodisperse liquid drops to form monodisperse liquid drops wrapping crystals, wherein a liquid film exists between the monodisperse liquid drops and the inner wall of the continuous crystallization part, and the liquid drops are not infiltrated with the inner wall of the continuous crystallization part. According to the micro-nano chemical chip, continuous crystallization can be realized, uniform and stable crystallization conditions are provided, and the situation that crystals are attached and deposited on the wall surfaces of the channels to block the channels is avoided.

The invention belongs to the field of droplet fusion, and particularly relates to a method for initiating different monodisperse droplet fusion by utilizing liquid wettability. The method comprises the processing steps of: (1) preparing disperse water phase, medium oil phase and continuous phase liquid; (2) injecting each disperse phase liquid into a micro-fluidic device to form water-in-oil emulsion droplets and oil-pocket water-in-oil emulsion droplets, enabling the formed water-in-oil emulsion droplets and the oil-pocket water-in-oil emulsion droplets to enter a collecting tube along with the continuous phase fluid; paving oil layers at the surfaces of the oil-pocket water-in-oil emulsion droplets at the surfaces of the water-in-oil emulsion droplets when the water-in-oil emulsion droplets contact the oil-pocket water-in-oil emulsion droplets in an expanded chamber of the collecting tube; and discharging the oil layers between water drops in the oil-pocket water-in-oil emulsion droplets and the water drops in the water-in-oil emulsion droplets, wherein the water drops in the two droplets are fused to achieve micro-mixing or micro-reaction; and (3) collecting the fused droplets.

The invention belongs to the technical field of fluid micro-dispersion and particularly relates to a micro-structural device for preparing mono-dispersed liquid drops and bubbles and a use method of the micro-structural device. The micro-structural device comprises a substrate, a cover plate and a capillary tube, wherein a step-shaped groove is formed in the substrate; the capillary tube is embedded into the upper half part of the groove, and dispersed phase liquid / air enters a main channel through the capillary tube; a continuous phase is in contact with a dispersed phase at a reducing position of the step structure, and the dispersed phase is sheared into the mono-dispersed bubbles and liquid drops. The micro-structural device is simple in structure, convenient to produce, less prone to blockage and large in operation range, and is not influenced by wettability of the channel; by setting parameters such as two-phase flow, the mono-dispersed liquid drops and bubbles of required sizes can be rapidly and accurately acquired; the micro-structural device is particularly applicable to the preparation of the mono-dispersed liquid drops and bubbles of 1-1000 microns and has good application prospects in fundamental research and practical industry.

The invention relates to a micro-fluid chip for decelerating fluid like droplets, cells and the like on the basis of the dielectrophoresis effect, and belongs to the technical field of micro-fluid chips. The micro-fluid chip comprises a substrate and a body structure arranged on and bonded with the substrate. The body structure comprises a micro-fluid pipeline for fluid circulating. An electrode I and an electrode II which are oppositely arranged are arranged outside the micro-fluid pipeline and on the substrate. The sides, close to the micro-fluid pipeline, of the electrode I and the electrode II are each in a square wave shape, and the other sides are each in a continuous belt shape. The micro-fluid chip has the advantages that the electrodes with special effects are added outside an existing micro-fluid conveying pipeline, due to the design of the electrodes, the aim of controlling the movements of droplets, cells and the like is achieved, more importantly, the electrodes are arranged on the two sides of a micro-fluid channel and do not extend into the micro-fluid channel, and through an active control method, damage-free control over single-dispersed droplets, biomolecules and molecules is achieved.