56 results about "Fluorescent staining" patented technology

The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.

The present invention pertains to a cancer cell identification method and a cancer cell identification system capable of identifying cancer cells via CTC detection by using an unstained image, and simply and highly accurately identifying and classifying cancer cells. The present invention comprises a CGAN 12 that performs image generation learning for generating a fluorescence estimation image 18 obtained by learning, in pairs, a fluorescence-stained image 8, which is obtained by fluorescently staining an inspection cell 2 via antibodies, and an unstained image 16 thereof, and then estimating the fluorescence-stained image 8 from the unstained image 16. The present invention also comprises a CNN 14 that performs image identification learning for receiving input of the unstained image 16 and the fluorescence estimation image 18 corresponding thereto as one set of cell information, performing machine-learning in advance as to whether the unstained image 16 is a cancer cell by using the fluorescence-stained image 8 of the inspection cell 2, and identifying whether the input unstained image 16 is a cancer cell. The CGAN 12 generates the fluorescence estimation image 18 from the unstained image 16, and the CNN 14 identifies whether the unstained image 16 obtained by imaging an inspected cell in blood is a cancer cell from the fluorescence estimation image 18 on the basis of the image identification learning, and then classifies the cell.

The invention provides application of a rice OsABI3 gene in reduction of rice cadmium accumulation, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The application comprises the following steps: directionally editing the OsABI3 gene by using a CRISPR / Cas9 technology, constructing a recombinant vector, transforming the recombinant vector into a rice callus by using an agrobacterium-mediated method, and screening to obtain a mutant plant. The rice OsABI3 gene is cloned and analyzed for the first time, the nucleotide sequence of the gene is disclosed, a new perspective is provided for illuminating an unknown molecular mechanism of rice for regulating and controlling Cd transportation, and the invention also proves that the OsABI3 gene has the capability of regulating and controlling Cd accumulation of rice; and tissue anatomy and fluorescent staining experiments prove that the OsABI3 has the capability of limiting radial transportation of Cd ions in a root system.

The invention belongs to the technical field of environmental analysis instruments and intelligent detection, and discloses a water sample micro-plastic detection device based on fluorescent staining and an AI algorithm, an integrated micro-fluidic chip is adopted to replace a traditional pretreatment process, and online digestion and fluorescence labeling of a water sample are automatically completed through a sequential injection analysis flow path; the configured high-sensitivity fluorescence imaging module can capture feature images of the micro-plastics; a built-in lightweight multi-scale feature fusion network model can identify and count micro-plastic targets, output quantity, particle size distribution and morphological parameters in real time. According to the invention, full-process automation from sample input to result output is realized, the problems of tedious pretreatment, high manual dependency and difficulty in on-site rapid quantification in the prior art are solved, and an efficient technical means is provided for water environment micro-plastic pollution monitoring.

The application discloses a method for constructing a cell membrane fusion model induced by a novel coronavirus-like particle, and belongs to the technical field of biology. The method comprises the following steps: constructing a virus-like particle containing a SARS-CoV-2 spike protein gene and an RNA packaging sequence PS9, using HEK-293T cells as packaging cells, co-expressing structural proteins M, E, N and S protein, and realizing assembly of VLPs without replication ability. Subsequently, the VLPs are used to infect immortalized type II alveolar epithelial cells, S protein expression is induced and cell-cell membrane fusion is mediated without the need of BSL-3 laboratory conditions. The formation of fused giant cells can be directly observed through ZO-1 protein immunofluorescence staining, the model is stable, has high biological safety, and is repeatable in vitro. The application overcomes the safety risk of live virus infection and the authenticity problem of a transient transfection model, can be used for research on a SARS-CoV-2 virus membrane fusion mechanism, and has a good application prospect.

The invention relates to the field of microbiological detection, and discloses a sample culture-free efficient identification kit and a detection process, the sample culture-free efficient identification kit comprises a staining reagent, a scattering calibration and inhibition reagent, a quality control reagent and consumables, the staining reagent is a multi-target fluorescent chromosome system, and the scattering calibration and inhibition reagent is a scattering calibration and inhibition reagent. The scattering calibration and inhibition reagent is used for double-scattering signal calibration and scattering noise inhibition, the quality control reagent is used for validity verification of a detection result, and the consumable is used for adaptive imaging detection. Through cooperative adaptation of a staining reagent, a scattering calibration and inhibition reagent, a quality control reagent and consumables, microorganism multi-target specific marking can be realized by means of a multi-target fluorescent chromosome system without depending on microorganism culture, and scattering signal interference is eliminated by combining the scattering calibration and inhibition reagent so as to guarantee the accuracy of detection signals. The validity of a detection result is verified through a quality control reagent, so that rapid identification and characterization of microorganisms in a complex sample matrix are realized.

The invention relates to the field of biotechnology and dead / living cell detection, in particular to a method for rapidly determining the activity of biological membrane cells in immobilized fermentation. According to the method, a methylene blue and trisodium citrate synergistic chromosome system is optimized, a biofilm EPS matrix is synergistically penetrated by virtue of the dispersion effect of trisodium citrate and the reduction characteristic of methylene blue, and a spectrophotometric method is combined to detect the OD value of a staining solution instead of stained cells, so that the efficient quantitative analysis of the ratio of dead cells to living cells of the immobilized biofilm is realized. The method can complete detection within 200 s, is simple and convenient to operate and low in cost, does not need complex equipment, and is remarkably superior to a traditional fluorescent staining method.

The invention discloses a device for observing migration of micro-nano iron particles in a porous medium, and belongs to the field of groundwater pollution remediation. The device comprises a liquid storage tank, a microfluidic system, an optical monitoring system, a receiving tank and a detection system. The liquid storage tank is used for storing a to-be-detected micro-nano iron particle material and carrying out fluorescent dyeing. The micro-fluidic system comprises an injection pump, a flow meter and a micro-fluidic chip, the micro-fluidic chip is made of a polydimethylsiloxane (PDMS) material and comprises a plurality of direct flow channels with liquid inlets and liquid outlets, and bionic porous network structures are designed in the channels. The optical monitoring system integrates an inverted fluorescence microscope and an sCMOS camera and controls monitoring records through computer software, and the receiving pool is used for collecting the nano-iron particle material flowing out of the micro-fluidic chip. The detection system is used for quantitatively testing and analyzing the content of the micro-nano iron in the receiving tank. The device can simply, quickly and accurately monitor the migration process of the micro-nano iron particles under different pore conditions.

The application discloses a fluorescent dye tracer method for tracking hydraulic fracturing cracks and application, and the method comprises the following steps: S1, configuring a fluorescent dye solution: using a water-soluble fluorescent dye rhodamine B to prepare a mass-volume ratio aqueous solution with a concentration of 0.01% to 0.05%; S2, pre-treating and cleaning the cracks of a sample: using a hydrochloric acid solution with a concentration of 1% to 3% to acid-wash the cracks of the sample, and then rinsing the sample with clean water; S3, pre-saturating the crack channel: pre-saturating the crack network of the sample; S4, injecting the fluorescent dye solution under low pressure pulse, so that the crack channel is filled with the fluorescent dye solution; S5, soaking and permeating: after the crack channel is filled with the fluorescent dye solution, stopping the injection and placing the sample, and soaking the sample to make the fluorescent dye solution penetrate into fine cracks and pores; S6, observing the dyeing: after the surface of the sample is dried, cutting the sample along the main crack direction to observe the internal crack direction, and performing quantitative analysis by using image processing software.

The invention relates to a blood cell analyzer and a blood cell analysis method. Sucking a to-be-detected blood sample; mixing a part of the blood sample to be detected, a hemolytic agent and a first fluorescent staining agent to prepare a first measurement sample, and detecting first optical information of particles in the first measurement sample; mixing the other part of the blood sample to be measured, the diluent and a second fluorescent staining agent to prepare a second measurement sample, and detecting second optical information of particles in the second measurement sample; acquiring first leukocyte classification information based on the first optical information; when it is judged that classification abnormity exists in the first leukocyte classification scatter diagram, second leukocyte classification information is obtained based on the second optical information, and the second leukocyte classification information is used for correcting the first leukocyte classification information. According to the invention, an accurate leukocyte classification result can be obtained.

This invention discloses a monoclonal antibody against Oit3 protein in mouse hepatic sinusoidal endothelial cells or its antigen-binding fragment and its applications, belonging to the fields of biotechnology and medical immunology. The monoclonal antibody or its antigen-binding fragment comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region contains HCDR1, HCDR2, and HCDR3 with amino acid sequences as shown in SEQ ID NO. 3~SEQ ID NO. 5; the light chain variable region contains LCDR1, LCDR2, and LCDR3 with amino acid sequences as shown in SEQ ID NO. 8~SEQ ID NO. 10. This monoclonal antibody and the recombinant fluorescent antibody exhibit high affinity and high specificity, and can be effectively applied to enzyme-linked immunosorbent assay (ELISA), Western blotting, and immunofluorescence detection. They can serve as important antibody tools for basic research on mouse hepatic sinusoidal endothelial cells and for exploring the mechanisms of liver diseases. Based on its variable region sequence, a recombinant fluorescent antibody, Oit3-scFv-GFP, was also constructed. This recombinant fluorescent antibody enables one-step direct immunofluorescence staining, providing a convenient tool for in situ visualization of mouse hepatic sinusoidal endothelial cells.

This invention discloses a method for detecting Candida albicans based on compound fluorescent staining and deep learning, belonging to the field of microbial detection technology. The method first synthesizes a novel NHC-triazolium salt fluorescent molecule, which is then compounded with calcium fluorescent white and DAPI to form a specific staining solution. After treating the sample with this staining solution, multi-channel fluorescence imaging is performed and images are acquired. Simultaneously, ITS sequencing is performed on the samples. Based on the sequencing results, the images are classified and bacterial cells are manually cropped to construct a labeled dataset. A convolutional neural network model is trained using this dataset to learn to recognize the unique "multi-fluorescent fingerprint" presented by Candida albicans stained with the compound staining solution. In application, the same staining and imaging are performed on the test samples, and the trained model automatically analyzes the images to achieve detection. This invention significantly improves the specificity of detection through the synergy of chemical staining and artificial intelligence. Experiments show that compared with traditional single staining or unstained methods, this method improves the specificity of Candida albicans recognition by 37% and 52%, respectively, and has the advantages of being fast, accurate, and highly automated.

This disclosure provides an image detection method, apparatus, electronic device, and storage medium. The image detection method includes: acquiring a multiplex immunofluorescence image to be tested, wherein the multiplex immunofluorescence image is imaging data after multiplex immunofluorescence staining of a tissue sample to be tested; performing tissue segmentation based on the multiplex immunofluorescence image to obtain a tissue region image; and performing image sharpness detection on the tissue region image to obtain a detection result. The image detection method provided by this application can effectively eliminate background noise interference in multiplex immunofluorescence images caused by staining at different locations in the image, greatly reducing the proportion of background in the input image for subsequent image detection, and improving the accuracy and reliability of the image detection results.

The invention provides a composition and a kit for oral squamous cell carcinoma typing and application of the composition and the kit, and particularly discloses the composition which comprises a fluorescent staining marker for CBX3 protein and a fluorescent staining marker for IL1RN protein. The invention also discloses a kit containing the composition. The kit and the composition can be used for oral squamous cell carcinoma typing, and prognosis and treatment sensitivity of a patient can be predicted according to a typing structure. The composition and the kit provided by the invention adopt a small amount of genes as classification and prediction indexes, so that the detection cost and the experiment complexity are greatly reduced. Through the established extremely simple classification rule, the expression quantity ratio of CBX3 to IL1RN only needs to be detected through the immunofluorescence technology, sample typing can be rapidly completed according to the condition that CBX3: IL1RN is larger than 1 or smaller than 1, complex biological information analysis is not needed, the detection process is visual and efficient, and the clinical diagnosis efficiency and accuracy can be effectively improved.

The utility model discloses a fluorescence generating device for a 3D printing wire rod, belongs to the technical field of 3D printing equipment, and aims to solve the problems that dust is easily attached to the outer surface of the printing wire rod to influence the fluorescence dyeing effect and the fluorescence generating device is difficult to uniformly dye the printing wire rod. A dyeing pipeline fixedly communicates with the outer wall of one side of the mounting box, a plurality of nozzles distributed circumferentially are arranged on the outer surface of the dyeing pipeline, a cleaning mechanism is arranged in the mounting box, and a dyeing assembly is arranged in the dyeing pipeline; the outer surface of the printing wire rod is cleaned through the cleaning brush, dust on the outer surface of the printing wire rod is reduced, fluorescent agent spraying is conducted on the outer surface of the printing wire rod through the spraying head, the coloring agent on the outer surface of the printing wire rod is evenly smeared through the smearing plate, and the situation that the outer surface of the printing wire rod is prone to being attached with dust to affect the fluorescent dyeing effect is effectively avoided; and a fluorescence generation device is not easy to uniformly dye the printing wire rod.

This invention discloses a TSA multiplex fluorescent staining kit, belonging to the field of staining kit technology. It includes a box body, with a placement plate fixedly connected to the inner wall of the box body. A placement cylinder is fixedly connected to the placement plate, a displacement block is slidably connected to the outer wall of the placement cylinder, a connecting plate is fixedly connected to the side wall of the displacement block, and a synchronization plate is fixedly connected to the top of the outer wall of the connecting plate. This invention, through the installation of the displacement block, clamping claws, and synchronization plate, achieves standardized liquid aspiration depth. The height of the clamping claws is synchronously adjusted with the height of the reagent bottle, solving the problem of inconsistent liquid aspiration depth caused by differences in reagent bottle height. This avoids the problem of drawing impurities or residue from the bottom of the bottle due to excessive aspiration, and the problem of drawing air bubbles or empty space due to insufficient aspiration, ensuring that clear and effective liquid is aspirated each time, thus improving the accuracy and repeatability of the experiment.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

This invention relates to the field of biodetection technology, specifically disclosing a multiplex fluorescent staining kit for tissue samples. The kit includes an antibody dissociation buffer, a signal amplification deposition solution, a fluorescent labeling reagent, a tissue-protective imaging medium, and an autofluorescence background inhibitor. This invention utilizes a non-destructive elution technique achieved through the synergistic effect of a low-pH buffer and a directional electric field. It leverages the positively charged nature of antibodies under acidic conditions to drive them away from the tissue and towards the negative electrode, achieving gentle and efficient antibody removal. Simultaneously, it employs a two-step cascade amplification strategy of small molecule deposition and copper-free click chemical labeling, balancing deep penetration with ultra-high sensitivity. This invention can stably support 6-12 cycles of staining, exhibits high elution efficiency, preserves tissue morphology, and can detect low-abundance targets. It is suitable for spatial analysis of the tumor microenvironment, monitoring of transplant immune rejection, and pathological evaluation of neurodegenerative diseases, demonstrating promising clinical application prospects.

This invention discloses a phosphate-containing compound, a phosphate-containing iridium complex, its preparation method, and its applications. The complex incorporates two phosphate groups through ligand modification, yielding a class of phosphate-containing cyclic metallized iridium complexes. These iridium complexes exhibit high water solubility and structural stability, and possess aggregation-induced emission properties. Their fluorescence emission is weak in the water-soluble state but significantly enhanced in the aggregated state. The introduction of phosphate groups gives these iridium complexes a high affinity for hydroxyapatite. This invention combines the fluorescence properties of iridium complexes with the affinity of phosphate groups for bone tissue, forming a product that can bind to the calcium matrix on the bone tissue surface, generating aggregation-induced emission, and can be used for fluorescent staining of bone tissue.

The invention relates to the technical field of virus detection, and discloses a multi-target fluorescent staining rapid sample preparation virus detection method, which comprises the following steps: S1, sample pretreatment; s2, multi-target fluorescent dyeing: mixing the pretreated sample in S1 with a multi-target fluorescent dye combination to obtain a dyed sample; s3, purification after dyeing; s4, optical imaging acquisition; s5, image analysis and judgment: inputting the fluorescence image in S4 into an image analysis system, extracting fluorescence characteristic parameters of each target spot, and comparing the fluorescence characteristic parameters with a preset threshold value; and S6, outputting a result. By adopting the technical scheme of combining multi-target fluorescent staining and multi-target co-localization judgment, the technical effects of accurately identifying target viruses and avoiding non-specific interference are achieved, and compared with the technical scheme of single-target fluorescent staining or single-signal judgment in the prior art, the technical scheme of the invention has the advantages that the accuracy is high; the problems that the specificity is insufficient, and false positive and false negative occur due to interference of environmental impurities or similar pathogens are solved.

The invention belongs to the field of nanocellulose, and particularly relates to a fluorescent cellulose nanocrystal based on a fluorescent fabric and a manufacturing process of the fluorescent cellulose nanocrystal. The manufacturing process comprises the following steps: S1, cutting the fluorescent fabric into fluorescent cloth pieces; s2, acid and the fluorescent fabric are mixed; and S3, after the reaction is finished, removing acid to obtain the fluorescent cellulose nanocrystal. According to the method, the fluorescent dyeing textile waste can be effectively recycled under the conditions that the structure of the covalent binding fluorescent dye is completely reserved and dissolution loss of the covalent binding fluorescent dye is avoided. Fluorescent components contained in the fluorescent fabric are effectively reserved in CNC, fiber resource utilization of the waste fluorescent fabric is achieved, pollution to the environment is reduced, meanwhile, the preparation cost of fluorescent CNC raw materials is reduced, and a new feasible way is provided for high-value-added resource utilization of the waste fluorescent fabric.

The invention relates to the technical field of fluorescence detection, in particular to a cell nucleus targeting yellow fluorescent probe and a preparation method and application thereof.The preparation method comprises the steps that meso-chlorine-substituted thio-piro red fluorescent dye is dissolved in a solvent and then reacts with alkyl primary amine for 6 hours at the room temperature, then the solvent is removed through rotary evaporation, separation and purification are conducted, and a target product is obtained; the yellow fluorescent probe capable of realizing specific high-contrast fluorescent staining on the cell nucleus is obtained. The method can be applied to real-time observation of changes of cell nucleus morphologies in different cell division cycles and rapid detection of cell nucleus morphological abnormalities of pathological cells in biological samples.

The present application relates to the technical field of high polymer materials, and discloses a near-infrared light-driven hydrogel actuator as well as a preparation method and application thereof.The near-infrared light-driven hydrogel actuator comprises a first hydrogel layer and a second hydrogel layer; the first hydrogel layer comprises the following raw materials: a polyacrylamide precursor solution, a poly(N-isopropyl acrylamide) precursor solution, an acrylic acid monomer and a fluorescent dye; and the second hydrogel layer comprises the following raw materials: a poly(N-isopropyl acrylamide) precursor solution, an acrylic acid monomer and a carbon nanomaterial.The near-infrared light-driven hydrogel actuator provided by the present application has an asymmetric bilayer structure constructed by poly(N-isopropyl acrylamide) and polyacrylamide, and the hydrogel can realize bending deformation under a thermal environment, so as to drive the hydrogel to move in water; and the hydrogel has fluorescent responsiveness and near-infrared light responsiveness, and can be used for heavy metal ion detection.

This utility model discloses a microbial and cell fluorescence staining detection kit, belonging to the technical field of reagent kits. It includes a box body with symmetrically arranged placement blocks inside. The box body also contains a storage component, and a fixing mechanism is located on the outer side of the box body. The storage component and the fixing mechanism work together. The storage component includes a storage cavity for fungal fluorescence staining solution bottles, which is located on one side of two placement blocks close to each other. T-shaped strips are fixedly connected to the inner wall of the box body in a rectangular array, and T-shaped grooves are formed in a rectangular array on the outer sides of the two placement blocks. This microbial and cell fluorescence staining detection kit, through its storage component and fixing mechanism, ensures correct use of the kit while preventing it from opening during transportation and movement, ensuring the kit's closure stability and transportation safety. It has a simple structure and strong practicality.

The invention relates to a blood cell analyzer and a blood cell analysis method. The method comprises the following steps: mixing a part of a sucked blood sample to be detected, a diluent and a fluorescent staining agent in a first reaction tank to prepare a first determination sample, and obtaining first optical information generated after particles in the first determination sample are irradiated by light; mixing the other part of the blood sample to be detected with a hemolytic agent in a second reaction tank to prepare a second determination sample, and obtaining colorimetric optical information of the second determination sample; obtaining a reticulocyte detection result, a platelet detection result, an erythrocyte detection result and a first leukocyte detection result of the to-be-detected blood sample based on the first optical information, wherein the first leukocyte detection result at least comprises a lymphocyte percentage, a mononuclear cell percentage and a granulocyte percentage; and determining the hemoglobin concentration of the to-be-detected blood sample based on the colorimetric method optical information. According to the application, the reagent cost required for obtaining the detection result of the to-be-detected blood sample can be reduced.

The invention discloses a sperm acrosome reaction detection method based on phase imaging, which comprises the following steps: preparing two sperm smears, obtaining a phase diagram SP of sperms without acrosome reaction, and obtaining a phase diagram SPacrosome of sperms with acrosome reaction; obtaining a sperm nucleus average phase value SPt, a sperm acrosome average phase value SPd, a sperm nucleus average phase value SPtarosome and a sperm acrosome average phase value SPdacrosome; calculating a first difference value between the SPt and the SPd and a second difference value between the SPtasrosome and the SPdarosome, and calculating a second difference value between the SPtasrosome and the SPdarosome; calculating the phase ratio R of the sperm acrosome to the sperm nucleus and the phase ratio Racrosome of the sperm acrosome to the sperm nucleus; and through comparative analysis of the SPt, the SPd, the R, the SPtarosome, the SPdacrosome and the Racrosome, a distinguishing and judging rule of the sperms without the acrosome reaction and the sperms with the acrosome reaction is obtained. The sperm acrosome reaction detection method based on phase imaging has the advantages that vacuole detection can be carried out without fluorescence staining on sperms, unmarked quantitative measurement on the sperms is realized, the detection efficiency and accuracy are high, and the like.

The subject of the invention is a microscope for high-resolution and specific analysis of biological substances.The microscope according to the invention is characterized by a power supply unit capable of generating constant current attached to the microscope housing; a set of mirrors and illuminating optics; a collimator placed between a monochromatic light source emitting light in a very narrow range of wavelengths and the collector lens; a dark-field condenser and objective including an in-between space to accommodate the stained biological specimen; and a surveillance camera or eyepiece, and a removable color filter placed between the objective and the surveillance camera or the eyepiece.The invention also covers the method to analyze biological specimens with fluorescent staining, wherein the biological contents of the specimen are analyzed in a growing medium in liquid state after the addition of antibodies marked with fluorescent stain, using the microscope according to the invention.The microscope according to the invention enables up to one order of magnitude higher resolution than conventional light microscopes and 10-30% higher resolution compared to currently used dark-field microscopes and provides a remarkably sharper camera image.

The utility model belongs to the technical field of kits, and particularly relates to a fungal D-glucan detection fluorescent staining kit device which comprises a kit body, the top of the box body is rotationally connected with a first cover plate; the middle part of the box body is fixedly connected with a bearing plate; a plurality of placement holes are formed in the middle of the bearing plate; a plurality of bearing cylinders are fixedly connected to the bottom of the bearing plate; a plurality of clamping plates are slidably connected to the middle of the bearing cylinder. An elastic film sleeve is fixedly connected to the middle parts of the plurality of clamping plates; a plurality of through holes are formed in the middle of the bearing cylinder; a placement groove is formed in the side wall of the box body; the top of the box body is rotationally connected with a second cover plate; a plurality of first through grooves are formed in the side wall of the placement groove; by means of the structure, after the fungal D-glucan detection fluorescent staining reagent bottle is put into the box, the reagent bottle can be extruded and fixed, the situation that the reagent bottle is loosened and shaken due to the small diameter is reduced, and the reagent bottle is more stable in the box.

The invention discloses an immunofluorescence staining method and device for biological tissues. The biological tissue immunofluorescence staining method comprises the steps of tissue fixation, tissue degreasing and immunostaining. The tissue degreasing comprises the step of degreasing the tissue by adopting a small-micelle zwitterionic surfactant; the immunostaining comprises the step of carrying out immunostaining under a low-voltage condition of 10-100V. According to the immunofluorescence staining method, the small-micelle zwitterionic surfactant is combined with low-voltage current for immunostaining, rapid and uniform staining of large-volume tissues such as mouse brains can be realized on the premise of not causing obvious tissue damage or expansion, and a fine structure is clearly displayed.

Proposed is a method for estimating the mass of microplastics by using fluorescent staining. The method may include staining microplastic samples by using a Nile red solution, and capturing a fluorescent image of each of the stained microplastic samples after classifying the stained microplastic samples by mass. The method may also include analyzing the captured fluorescent image to quantify a fluorescence intensity thereof to build a database (DB) about a correlation between mass and fluorescence intensity of microplastics. The method may further include estimating a mass value of an unknown microplastic sample by comparing a fluorescence intensity of a fluorescent image of the unknown microplastic sample obtained by performing the staining and capturing of the unknown microplastic sample with data stored in the database.