A method and apparatus for identifying a difficult-to-differentiate bacterial species, a device, and a medium, relating to the technical field of microbial identification, and comprising: acquiring a
mass spectrometry identification result sent by a source
mass spectrometer, determining whether a difficult-to-differentiate bacterial species is present in the
mass spectrometry identification result, and if the difficult-to-differentiate bacterial species is present in the
mass spectrometry identification result, determining whether there is a fine-grained
differential analysis model corresponding to a type of the difficult-to-differentiate bacterial species in a model
library; if there is a fine-grained
differential analysis model corresponding to the type of the difficult-to-differentiate bacterial species in the model
library, invoking the fine-grained
differential analysis model to analyze and identify the difficult-to-differentiate bacterial species so as to obtain a model analysis and identification result; if there is no fine-grained differential analysis model corresponding to the type of the difficult-to-differentiate bacterial species in the model
library, using a dynamic matching
algorithm to perform dynamic computational identification on the difficult-to-differentiate bacterial species, or using the dynamic matching
algorithm and invoking the fine-grained differential analysis model to perform dynamic computational identification on the difficult-to-differentiate bacterial species to obtain a dynamic computational identification result, thereby improving matrix-assisted
laser desorption time-of-flight mass
spectrometer accuracy and efficiency with respect to identifying similar microorganisms.