41 results about "Animal imaging" patented technology

An apparatus for imaging an animal includes a first mounting surface, a bed sized to support the animal and releasably secured to or integral with the first mounting surface. The apparatus also includes a plurality of straps, each having a first end in a fixed position relative to the bed and a second end for tightening around a limb of the animal. A method for in-vivo imaging of an animal includes providing an animal that has limbs, providing a first mounting surface, and providing a bed removably secured to or integral with the mounting surface and sized to support the animal as well as being coupled to a plurality of straps. The method also includes placing the animal on the bed between the plurality of straps and tightening at least two of the plurality of straps around at least two of the limbs such that the animal is substantially secured in place relative to the bed.

The invention discloses a living small animal imaging system in a miniature CT and fluorescent tomography dual mode and an imaging method thereof. The living small animal imaging system in the miniature CT and the fluorescent tomography dual mode comprises a main control computer, an X-ray source and an X-ray detection device, an excitation source and an excitation light / fluorescent light detection device, and a rotary scanning device, wherein the X-ray source and the X-ray detection device, the excitation source and the excitation light / fluorescent light detection device, and the rotary scanning device are controlled by the main control computer. The invention can simultaneously acquire structure information by carrying out the miniature CT imaging on a small animal and fluorescent-labeled molecular information by carrying out the fluorescent tomography on small animals to enable the molecular information acquired by the fluorescent tomography to be accurately positioned in the smallanimal, thereby being beneficial to improving the diagnosis accuracy.

The invention relates to an amphiphilic illuminant with aggregation induced emission characteristics and applications thereof. The illuminant is prepared by connecting a hydrophilia unit on a classical hydrophobicity unit of the classical aggregation induced emission characteristics (AIE), and can be applied to a fluorescent light chemical sensor and is used for preparing fluorescent light coloring agent which is used for coloring living cells and animal imaging fluorescent light. The amphiphilic coloring agent is specifically suitable for the fluorescent light mark on biopolymer, and can be used as a biocompatibility probe for AIE activation so that the amphiphilic coloring agent can be applied to clinic cancer imaging, diagnose and treatment.

The invention discloses a collimator device for small animal imaging, which comprises a scanning bed, a motion control platform, a collimator and an imaging detector, wherein the scanning bed is used for supporting and fixing an object to be detected; the motion control platform is used for controlling the scanning bed to move in a predetermined scanning track; the collimator is used for limiting the angles of radioactive rays emitted by each fault of the object to be detected; and the imaging detector is used for receiving the radioactive rays emitted by the object to be detected and limited with the angles through the collimator, forming an inverse amplified projection image of the object to be detected thereon, acquiring projection data of each fault of the object to be detected and performing fault reconstruction to acquire a three-dimensional fault image of the object to be detected. The collimator device has high flexibility by selecting different pinhole inserts and collimator plates and adjusting the distance between the collimator and the imaging detector, can realize multi-mode imaging of high-resolution imaging, high detection efficiency imaging, large-view small animal imaging and the like, and has imaging high resolution and low cost.

An apparatus for imaging an animal includes a first mounting surface, a bed sized to support the animal and releasably secured to or integral with the first mounting surface. The apparatus also includes a plurality of straps, each having a first end in a fixed position relative to the bed and a second end for tightening around a limb of the animal. A method for in-vivo imaging of an animal includes providing an animal that has limbs, providing a first mounting surface, and providing a bed removably secured to or integral with the mounting surface and sized to support the animal as well as being coupled to a plurality of straps. The method also includes placing the animal on the bed between the plurality of straps and tightening at least two of the plurality of straps around at least two of the limbs such that the animal is substantially secured in place relative to the bed.

The invention facilitates transport of an immobilized, anesthetized small animal across multiple single-modality or multiple-modality imaging workstations at the same or different physical locations without loss of subject positional information. The animal holder is compatible with preclinical animal imaging stations such as micro-CT, micro-MR, micro-PET, micro-SPECT, and FMT. The animal holder is configured to be accommodated by (for example, fit within) individual imaging chambers of such instruments and is fabricated from materials that are compliant with all of the imaging modalities used. In certain embodiments, an integrated set of fiducial marker wells accommodates the dispensing of markers that are picked up by several modalities simultaneously in multiple planes. The fiducial markers then are aligned in standard image processing or image analysis software with simple image translation and rotation operations, without the need for more advanced scaling, distortion or other operations.

The invention relates to a magnetic resonance imaging superconducting magnet for animal imaging. The superconducting magnet is composed of superconducting main coils (2), superconducting axial shielding coils (3) and a superconducting radial shielding coil (4), wherein all the coils are axisymmetric solenoid coils using a symmetric axis (8) as a center axis. The superconducting main coils (2) are three pairs of solenoid coils with forward current, and the superconducting axial shielding coils (3) are a pair of solenoid coils with reverse current. The superconducting main coils (2) and the superconducting axial shielding coils (3) are positively symmetrically arranged about a symmetry plane (9). The superconducting radial shielding coil (4) is a solenoid coil with the reverse current, and the middle plane of the superconducting radial shielding coil is overlapped with the symmetry plane (9). The superconducting magnet generates highly-uniform magnetic field distribution at three concentric spherical areas (5, 6 and 7) with the diameters respectively being 80mm, 150mm and 200mm, and five Gaussian stray fields are respectively limited within the range of 1.5m and 2.2m ellipsoids in a radial direction and an axial direction.

A multi-axis imaging system and methods for imaging a human or animal using a multi-axis imaging system are provided.

The invention discloses an animal PET / CT (positron emission tomography / computed tomography) imaging quality detection phantom. The quality detection phantom comprises a phantom main body in the form of an animal, wherein at least one detachable substitute module is arranged in the phantom main body; and the detachable substitute module is selected from one or more of the following modules: an in-vivo tumor simulation module, a PET / CT image fusion degree test module, a PET / CT attenuation correction test module, a PET performance parameter test module and a CT performance parameter test module. The phantom provided by the invention is a quality detection phantom having morphological features; the phantom can simulate appearances, structures and focuses of real animals; the phantom is applicable to quality control of an animal PET / CT imaging instrument; and in addition, the phantom can be used for searching optimum scanning parameters of the PET / CT imaging instrument and is applicable to such aspects as teaching demonstration, tracer agent screening and the like; therefore, the phantom has a broad application scope.

An encapsulated chelate dendritic polymer and an encapsulated ligand dendritic polymer are disclosed which have unique properties. These encapsulated chelate dendritic polymers may have associated with its dendritic polymer surface target directors, proteins, DNA, RNA (including single strands) or any other moieties that will assist in diagnosis, therapy or delivery of this encapsulated chelate dendritic polymer. These encapsulated dendritic polymers are suitable as contrast agents for use in imaging in an animal, for other imaging techniques, for EPR, and as scavenger agents for chelant therapy. Formulations for these uses are also included within the scope of this invention.

The invention relates to the field of magnetic resonance imaging, and particularly relates to a magnetic resonance imaging system. The magnetic resonance imaging system comprises a bed board and a support, wherein the bed board comprises a groove, the support is arranged in the groove, a slide groove is arranged at one side, facing the bottom of the groove, of the support, and the magnetic resonance imaging system also comprises a head coil which is at least partially arranged in the groove, and the head coil can slide in the slide groove; and / or a spine coil which is at least partially arranged in the groove, and the spine coil can slide in the slide groove; and / or an abdomen coil which is detachably connected with any one of the bed board, the support and the spine coil. When in animal imaging, animals do not need to be moved, and the imaging can be achieved only by moving the coil, so that convenience in imaging can be realized, and the whole-body imaging can be realized.

Belonging to the fields of pharmacy and clinical pharmacy, the invention relates to a folic acid modified and vincristine encapsulated active targeting liposome drug delivery system, a preparation method and application in multidrug resistant tumor targeted drug delivery. The invention discloses a preparation method of a folic acid modified and vincristine encapsulated liposome. Cell specific uptaking and living animal imaging tests show that the drug delivery system has good in vitro and in vivo tumor cell targeting. 3-(4, 5-dimethylthiazole)-3, 5-diphenyltetrazolium bromide (MTT) analysis indicates that the drug delivery system has a good effect for inhibiting the in vitro growth of tumor cells. Pharmacodynamic results show that the drug delivery system has a good effect for inhibiting the in vivo growth of multidrug resistant tumors. The drug delivery system can enter the whole body blood circulation through intravenous injection dosing, then can be targeted to multidrug resistant tumor sites through tumor EPR effect and folic acid mediation and enter the tumor cells.

The invention discloses a rotary imaging system, a plant imager, an animal imager and an animal and plant imager, and belongs to the technical field of living body sample imaging. In the invention, the plant imager, the animal imager and the animal and plant imager respectively comprise a rotary imaging system, and each rotary imaging system comprises a sample stage unit, a camera unit and a rotating unit; the sample table unit is used for bearing samples; the camera unit is used for imaging the sample; and the rotating unit comprises a containing cavity containing the sample table unit, and the rotating unit is used for driving the camera unit to rotate relative to the sample table unit and controlling the camera unit to be static relative to the sample table unit. Through the rotary imaging system, powerful data support is provided for subsequent image reconstruction and image fusion. The number of cameras required for imaging different positions of the sample is also reduced.

An imaging system that allows for unhindered access to a sample to be imaged. A housing structure is configured with a drawer having a platform. The platform may be configured to hold a sample, such as an animal, and / or the platform may be configured to mate with a sample holding member, such as a removable carrying tray for holding the sample. The drawer presents the platform to an imaging device or system internal to the housing when in a closed, or imaging, state. When in an extended state, the drawer presents the platform external to the housing to allow unobstructed manipulation of a sample on the drawer platform (or on the sample holding member on the platform). When used, a sample holding member such as a removable carrying tray may be docked with the drawer platform, and various interconnects, such as gas ports and electrical connectors, on the sample holding member engage with corresponding elements on the platform when docked. The tray may be undocked and moved to a remote location having a compatible docking station to allow for preparation or processing of a sample elsewhere. The tray may be docked with a docking station located at a sample preparation station. In the case of a live animal sample, the preparation station may include a sterile hood or other laboratory location.

The invention discloses a rodent small animal imaging device for an ultrahigh-field magnetic resonance imaging system. The rodent small animal imaging device comprises a cylindrical coil supporting shell, a hydrogen nucleus transmitting and receiving coil and a non-hydrogen nucleus transmitting and receiving coil, wherein the hydrogen nucleus transmitting and receiving coil and the non-hydrogen nucleus transmitting and receiving coil are arranged in a shell wall interlayer of the coil supporting shell; each of the hydrogen nucleus transmitting and receiving coil and the non-hydrogen nucleus transmitting and receiving coil comprises a front coil unit and a rear coil unit which are both of an annular structure and are arranged at intervals, and a plurality of straight wires which are connected with the front coil unit and the rear coil unit and are arranged at intervals in the circumferential direction. The hydrogen nucleus transmitting and receiving coil and the non-hydrogen nucleus transmitting and receiving coil are arranged in a sleeving mode, and each straight wire of the hydrogen nucleus transmitting and receiving coil is arranged between two corresponding adjacent straight wires on the non-hydrogen nucleus transmitting and receiving coil. The device provided by the invention can obtain a magnetic resonance image with higher quality and more comprehensive information.

The invention discloses a transgenic construct and application thereof in preparation of a p53 fixed point knock-out and bifluorescence report gene tissue specific expression gene editing mouse. All mTrp53 gene segments on a chromosome 11 of the mouse are knocked out at fixed points, and meanwhile, gene segments with the same length are knocked into the sites, so that the expression of a firefly luciferase gene is regulated by an endogenous p53 gene promoter; the expression of mCherry depends on the tissue specific expression of Cre recombinase. A gene fixed point knock-out / knock-in molecularimage mouse model is prepared for researching a p53 gene transcriptional regulation mechanism; the influence on mouse heredity, development and growth, and tumour occurrence and metastasis caused by p53 gene knockout is monitored in real time noninvasively by utilizing a small animal imaging system, so that the organ selective molecular mechanism of the tumour occurrence is researched, the organ targeting ability of a tumour treatment medicament is observed, and an important model for evaluating tumour medicament treatment pharmacodynamics is established; therefore, the transgenic construct has the application value.

The invention provides a preparation method of a chitosan molecular beacon nanocomposite. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite is synthesized by a self-assembly method. The chitosan miR-155 (microRNA-155) molecular beacon nanocomposite can dynamically monitor the occurrence and development processes of lung cancer according to the fluorescence intensity. At the same time, the expression of miR-155 can be further inhibited by combination with the miR-155 highly expressed in cancer cells, so as to achieve the purpose of inhibiting tumor; the nanocomposite can be applied in living animal imaging and tumor therapy of lung cancer cells and lung cancer mice with tumor, has the characteristics of high transfection efficiency, high specificity, good stability, low background and high inhibition rate, can provide new ideas, new methods and new technologies for early-stage diagnosis and treatment of lung cancer.

The invention discloses a method for constructing a mouse model capable of specifically knocking out an IKKalpha gene in a hippocampus region, a targeting vector and a kit. The IKKalpha gene is knocked out from specific mouse tissues through utilizing a Cre-LoxP recombination system, so that a transgenic mouse with the knocked-out IKKalpha gene is established, and an NF-omicronB signal channel and an acting mechanism on diseases, particularly tumors, are researched on the whole level of animals. Meanwhile, in order to determine and monitor whether specific knockout of the IKKalpha gene in the hippocampus region causes influences on the behavioral science of the mouse, red luciferase RFP720 is connected into a transgenic carrier. A gene targeting technology is adopted, and expression of the endogenous IKKalpha gene in the hippocampus region of the mouse is reduced based on iRFP720 near-infrared fluorescent protein and a Cre-LoxP gene knockout system, so that living body imaging of the transgenic mouse is monitored through an animal imaging system.

The invention discloses a lymphatic choriomeningitis virus for expressing a luciferase gene as well as a construction method and application of the lymphatic choriomeningitis virus, and relates to the technical field of biology. The luciferase gene is inserted between the 5 'UTR of the LCMV virus genome and the N end of the NP protein sequence to construct the virus genome marked by the luciferase reporter gene, the result does not affect protein processing modification after virus translation, and the generated luciferase protein does not affect assembly of filial generation virus particles. A sequence containing a recombinant virus genome is inserted into a vector and transfected with a host cell, so that a recombinant virus capable of efficiently and stably expressing luciferase can be saved, and the luciferase recombinant virus strain can be used for establishing a rapid and high-throughput antiviral drug screening technology or platform by virtue of a mouse bioluminescence in-vivo imaging system. Wide application values are provided for living animal imaging, drug screening, drug action mechanism, vaccine evaluation and the like.

The invention discloses a small-sized desktop type magnetic resonance quality control and comprehensive test phantom which comprises a shell, wherein a test liquid and an independent modular test module are arranged in the shell; and the test module comprises a three-dimensional test module, a layer thickness test module, a relaxation test module, a contrast resolution test module, a location test module and a geometric distortion test module. The small-sized desktop type magnetic resonance quality control and comprehensive test phantom is characterized by further comprising a chemical shift test module, a movement test module and a coil probe uniformity and linearity test module. The phantom is applicable to the test of small-sized desktop type animal imaging magnetic resonance apparatuses and magnetic resonance imaging systems, and has the advantages of multiple parameters, high precision, combination type and flexible pore diameter design.

The invention provides improved red fluorescin and an application thereof and belongs to the field of protein engineering. The improved red fluorescin RFPSpark provided by the invention is obtained through reforming an amino acid sequence eqFP578 of red fluorescin derived from Entacmaea quadricolor and has an amino acid sequence shown in SEQ ID No. 7. Compared with wild type protein, the fluorescin has obviously different excitation and emission spectra and has the characteristics of higher fluorescence intensity, higher mature speed, better stability and longer wavelength; and compared with another modified protein FP650 of eqFP578, the fluorescent brightness of the red fluorescin provided by the invention is 3 times higher than that of the FP650 under equivalent test conditions. The invention further provides the application of the improved red fluorescin. The improved red fluorescin is applicable to analysis of cell marking, animal deep tissue and living animal imaging, multicolor application and the like and has a broad application prospect.

The invention relates to an amphiphilic illuminant with aggregation induced emission characteristics and applications thereof. The illuminant is prepared by connecting a hydrophilia unit on a classical hydrophobicity unit of the classical aggregation induced emission characteristics (AIE), and can be applied to a fluorescent light chemical sensor and is used for preparing fluorescent light coloring agent which is used for coloring living cells and animal imaging fluorescent light. The amphiphilic coloring agent is specifically suitable for the fluorescent light mark on biopolymer, and can be used as a biocompatibility probe for AIE activation so that the amphiphilic coloring agent can be applied to clinic cancer imaging, diagnose and treatment.

The invention discloses a construction method of an acute lymphocytic leukemia mouse model. The construction method comprises the following steps of preparing leukemia cell line suspension with high expression of ERG, knocking down ERG protein expression of the cell line by using shERG packaged by lentivirus, transfecting Luciferase into a wild type and shERG cell line by lentivirus, inoculating the cell line into an immunodeficient mouse, evaluating the tumor process by using a small animal imaging system, and evaluating the treatment effect of a specific drug on the acute lymphocytic leukemia with high expression of ERG by using the small animal imaging system and a flow analysis method, and the like. The model evaluates the efficacy of a specific drug on a subtype of acute lymphocytic leukemia. According to the disease model construction method provided by the invention, the tumor development process of the model animal can be directly and noninvasively evaluated, and the success rate is very high. In addition, the disease model established according to the method provided by the invention has very high clinical correlation.

The device for screening for bioactive materials using the visual recognition of animals according to the present invention includes: an accommodating member (100) in which animals administered with a candidate bioactive material are accommodated; an imaging member (200) which captures images of the animals; and a detecting member (300) which reads the images in order to detect the visual recognition reactions of the animals. According to the method of the present invention for screening for bioactive materials using visual recognition of animals, the animals are administered with the candidate bioactive material, and the visual recognition reactions of the animals are detected.

The invention relates to a body temperature disorder detection system based on infrared induction, and the system comprises a room temperature identification mechanism which is disposed in a cold blood animal living hall and is used for detecting the air temperature of the environment where the room is located, and outputting the air temperature as the current air temperature in the living hall; an infrared camera shooting mechanism which is used for executing infrared camera shooting operation on an internal scene of the cold blood animal living hall; and numerical analysis equipment which is used for sending out a body temperature disorder instruction when the deviation difference value between the animal surface temperature corresponding to a certain animal imaging pattern and the current in-museum air temperature is greater than or equal to a preset difference value threshold value in the received tail end mapping picture. The body temperature disorder detection system based on infrared induction is synchronously operated, and is simple and convenient. The intelligent analysis mode can be adopted to detect whether the body surface temperature of various different types of cold blood animals living in the cold blood animal living library is out of control or not in real time, so that the labor cost of cold blood animal nursing is saved.

The invention discloses a smart human body induction device applied to a toilet and an application method thereof. The smart human body induction device comprises a general switch, a millimeter wave detection module, a comparison module, a control module, a circuit protection module, a time delay module, an exhaust fan, a photosensitive induction module and a lighting lamp. The general switch canswitch on and switch off operation of the smart human body induction device. The millimeter wave detection module is used for sensing humans or animals in the toilet and performing imaging. The comparison module determines whether there is a human according to an imaging frame of an accessing animal detected by the millimeter wave detection module. The control module is used for controlling poweron and power off of the circuit protection module. The circuit protection module is used for protecting the normal operation of a circuit. The time delay module is used for timing and enabling time-delayed power off of an exhaust fan circuit. The photosensitive induction module is used for sensing the intensity of external light and controlling the switching on and off of the lighting lamp. The exhaust fan is used for exhausting air and circulating air. The lighting lamp is used for lighting. The smart human body induction device is convenient and practical, does not require manual operation,is energy-saving and environmentally-friendly, and reduces unnecessary loss.