Construction method of acute lymphocytic leukemia mouse model

Abstract

The invention discloses a construction method of an acute lymphocytic leukemia mouse model. The construction method comprises the following steps of preparing leukemia cell line suspension with high expression of ERG, knocking down ERG protein expression of the cell line by using shERG packaged by lentivirus, transfecting Luciferase into a wild type and shERG cell line by lentivirus, inoculating the cell line into an immunodeficient mouse, evaluating the tumor process by using a small animal imaging system, and evaluating the treatment effect of a specific drug on the acute lymphocytic leukemia with high expression of ERG by using the small animal imaging system and a flow analysis method, and the like. The model evaluates the efficacy of a specific drug on a subtype of acute lymphocytic leukemia. According to the disease model construction method provided by the invention, the tumor development process of the model animal can be directly and noninvasively evaluated, and the success rate is very high. In addition, the disease model established according to the method provided by the invention has very high clinical correlation.

Description

technical field [0001] The invention relates to the field of leukemia disease models and construction methods thereof, in particular to a construction method of acute lymphocytic leukemia mouse models. Background technique [0002] Acute Lymphoblastic Leukemia (ALL) is a common and life-threatening malignant tumor in children. In children less than 10 years old, the incidence rate is about 3 / 100000-4 / 100000). According to the different cell types, it can be divided into B-cell type (B-ALL) and T-cell type (T-ALL). The current treatment options for ALL include risk-adapted treatment options, including combined chemotherapy, targeted therapy, and hematopoietic stem cell transplantation. Combination chemotherapy mainly uses vinblastine, cytarabine, methotrexate and so on. Due to the continuous improvement of treatment techniques, the prognosis of ALL has been greatly improved, and the 5-year survival rate of ALL patients has reached 80%. However, there are still 20% ALL pat...

AI Technical Summary

Problems solved by technology

[0003] Mice are ideal animal models commonly used in leukemia research, and corresponding mouse models can be obtained for malignant tumors such as myeloid and lymphatic systems in the human blood system, but now Some humanized mouse models still have defects such as low construction success rate and low clinical relevance. It is extremely important to construct leukemia models with high specificity and high anthropomorphism for leukemia research

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