The invention relates to the technical field of animal detection, in particular to a fixing device for living
small animal detection, which comprises a base, a negative
pressure control host arranged on the base, a pedal and a buffer
bottle arranged on two sides of the negative
pressure control host, and a hose for connecting the pedal, the buffer
bottle and the negative
pressure control host, a detection mechanism is arranged on the mounting table, the detection mechanism comprises a detection mounting seat arranged on the mounting table, an object placing table and a rotary supporting ring which are arranged on the detection mounting seat, and a probe adapter mounted on the rotary supporting ring, one end of the probe adapter is connected with a buffer
bottle through a hose, and the other end of the probe adapter is connected with an air cylinder; through cooperation of the transverse moving
assembly and the lifting lead screw, two-degree-of-freedom adjustment of the probe adapter in the horizontal direction and the vertical direction is achieved, the imaging stability is ensured, and the problem of
motion artifacts caused by focal plane variation of a traditional device is effectively solved.