979 results about "Small animal" patented technology

The invention belongs to the technical field of transformer substation small animal driving, and particularly discloses a comprehensive small animal driving device and method.The comprehensive small animal driving device comprises a device shell, a driving module, a power supply module, a detection module and a control module, and the detection module is arranged on the device shell and used for judging whether small animals invade or not based on infrared detection; the detection module is arranged on the device shell and sends a detection signal to the control module, and the driving module is arranged on the device shell, is connected with the control module and is used for driving invading small animals from multiple aspects of vision, hearing, smell and touch; the power supply module is arranged on the device shell, connected with the driving module, the detection module and the control module and used for supplying power to all the modules. According to the comprehensive driving device and method for the small animals, multiple driving modes are adopted for cooperative operation, the small animals are driven from multiple angles and multiple aspects, and therefore the reliability of driving operation is guaranteed.

The invention provides a water outlet control method and device, a mineralization faucet and a storage medium, and the method comprises the steps: carrying out the human body recognition of a target object in a current space, and obtaining a target recognition result; when determining that the target object is a non-human animal object based on the target identification result, determining that the water outlet control strategy is a water outlet prohibition strategy; when it is determined that the target object is the human body object of the preset age group based on the target recognition result, the water outlet control strategies are determined as a low-temperature secondary unlocking water outlet strategy and a high-temperature multi-time unlocking water outlet strategy; and when it is determined that the target object is a human body object of a non-preset age group based on the target recognition result, determining that the water outlet control strategies are a low-temperature direct water outlet strategy and a high-temperature secondary unlocking water outlet strategy. Through accurate human body recognition and water outlet control strategies, small animals are effectively prevented from mistakenly triggering water outlet, different water outlet control modes are provided for users of different age groups, and the water outlet safety is improved; through real-time monitoring and visual display of water quality information, a user can obtain instant water quality feedback, the convenience and accuracy of drinking water quality monitoring are improved, and thus the user experience is improved.

The invention relates to the technical field of cable branch boxes, and discloses a cable branch box which comprises a box body and a box door, the box door is rotatably connected to the box body, a placement groove is formed in the box body, a through hole communicated with the placement groove is formed in the box body, and an elastic plate used for completely blocking the through hole is arranged on the through hole. According to the device, the through hole is blocked through the elastic plate, so that small animals are difficult to directly pass through the through hole to enter the placement groove; when a worker cuts the elastic plate, the cable enters the placement groove through the elastic plate, and the elastic plate abuts against the outer surface of the cable, so that the elastic plate can limit the distance between the cable and the through hole, thereby reducing the possibility that a small animal climbs into the placement groove along the cable, and further reducing the damage of the small animal to the electrical component.

The invention discloses a wearable small animal electrical stimulation device, and belongs to the technical field of medical instruments, the purpose of connection is achieved by plugging a wiring terminal into an electric plugging seat, a pressing ring extrudes a piston cylinder after being pressed, gas is guided into a gas cushion ring through a gas pipe, and the gas cushion ring is connected with the wiring terminal. The air cushion ring expands and drives a plurality of sealing rings to be embedded into a sealing groove outside the power connection socket, the purpose of sealing connection between the fixing sleeve and the power connection socket is achieved, the situation that water seepage and dust accumulation are caused by a gap between the fixing sleeve and the power connection socket is prevented, and the end of a clamping strip in the locking assembly can retract into a sliding way after being pressed, so that the sealing effect is improved. When the clamping strip moves to the clamping groove, the clamping strip can be clamped into the clamping groove through elastic support of the second spring, so that the purpose of locking the position of the electric socket is achieved, and the situation that the electric socket and the wiring terminal are loosened due to the fact that a small animal is subjected to stress reaction when being electrically stimulated and a wire is pulled is prevented; and the use stability and the experiment precision of the electrical stimulation device are ensured.

The invention discloses a multi-modal imaging system and method for a small animal living body. The system comprises a multi-modal fusion imaging module which is responsible for multi-modal image acquisition and primary processing; the real-time dynamic monitoring module is used for capturing bioluminescence / fluorescence signals in real time through a photon counting detector, receiving optical, nuclear medicine and magnetic resonance data, correcting motion artifacts based on a multi-scale space-time registration engine and adjusting scanning parameters through a real-time pharmacokinetic-physiological feedback mechanism; the low-radiation and biological compatible module is used for automatically optimizing the dosage of a tracer agent according to the weight of the animal, the scanning part and historical data by using a dosage prediction model; and the multi-modal data fusion module is used for performing non-rigid registration of multi-modal images based on a Transform cross-modal registration network, constructing a multi-species pharmacokinetic knowledge graph by utilizing multi-species metabolism chip data, integrating mouse, dog, primate and humanized liver chip data, and mining a cross-species metabolism rule through a graph neural network.

The invention provides an animal behavior training device based on an air floating ball before magnetic resonance imaging, and particularly relates to the technical field of experimental equipment.The animal behavior training device based on the air floating ball before magnetic resonance imaging comprises a supporting mechanism and a floating ball mechanism, an air floating ball base clamping groove is formed in the supporting platform; the floating ball mechanism comprises an air floating ball base arranged in the air floating ball base clamping groove, a semi-circular groove is formed in the air floating ball base, an air floating hole is formed in the bottom of the semi-circular groove, an air floating ball is loaded in the semi-circular groove, a plurality of air inlet pipe openings communicated with the air floating hole are formed in the bottom of the air floating ball base, and the air inlet pipe openings are communicated with the air floating hole. An external air blower is utilized to ventilate air into the air floating holes through the air inlet pipes and drive the air floating balls to suspend, and the four limbs of the small animal can move on the air floating balls in a multi-degree-of-freedom mode and are not limited.

The utility model relates to a high-pressure device suitable for long-time living of small animals, which comprises an in-cabin animal bearing mechanism and an in-cabin pressure sensor which are positioned in a high-pressure cabin body, the outside-cabin air purification mechanism, the outside-cabin dirt flushing and collecting mechanism, the outside-cabin food supply mechanism, the outside-cabin water supply mechanism, the outside-cabin high-pressure air supply mechanism and the outside-cabin controller are located outside the high-pressure cabin body, and the outside-cabin high-pressure air supply mechanism is connected and communicated with the high-pressure cabin body, the outside-cabin dirt flushing and collecting mechanism, the outside-cabin food supply mechanism and the outside-cabin water supply mechanism. The cabin exterior air purification mechanism is connected and communicated with the hyperbaric cabin body, the cabin exterior dirt flushing and collecting mechanism is located under the cabin interior animal bearing mechanism and is connected and communicated with the cabin interior animal bearing mechanism, and the cabin exterior feeding mechanism is located over the cabin interior animal bearing mechanism and is connected and communicated with the cabin interior animal bearing mechanism; the out-cabin water supply mechanism is located over the in-cabin animal bearing mechanism and communicated with the in-cabin animal bearing mechanism.

The invention relates to the technical field of transformer substation small animal monitoring and early warning, and discloses a transformer substation small animal intrusion monitoring and early warning method and system, and the system comprises an image preprocessing module considering micrometeorological factors, an intrusion target detection module based on image recognition and target tracking, and a track prediction module without a monitoring area. According to the method, an improved YOLOv5s algorithm is adopted as a reference detection model, an improved EIOU loss function is adopted for replacement for the defect of a loss function in the model, meanwhile, the defect that irregular target features are difficult to extract in common convolution is overcome through a mode of replacing deformable convolution, the recognition accuracy of the model is improved, and the recognition efficiency of the model is improved. Therefore, the target in the transformer substation is detected, whether the target is an invading small animal or not is judged, the recognition time is effectively shortened, meanwhile, the accuracy is improved, and the increasing intelligent requirement is met.

The invention relates to a 60KW energy storage equipment energy-saving ventilation and noise reduction structure capable of being efficiently and rapidly assembled. The 60KW energy storage equipment energy-saving ventilation and noise reduction structure comprises a ventilation static pressure structure, an air inlet noise elimination structure, an air exhaust noise elimination structure, arc-shaped noise elimination blades, an insertion type noise elimination module, an anti-bird net and a rapidly-assembled hanging type hinge structure. The ventilation static pressure structure homogenizes airflow through the static pressure cavity, reduces resistance and improves ventilation efficiency; according to the air inlet and exhaust noise elimination structure, noise is effectively absorbed through trapezoidal noise elimination pieces and arc-shaped noise elimination blades, and comprehensive noise reduction of low-frequency and medium-high-frequency noise is achieved by combining inclined design of the noise elimination pieces with optimization of the punching rate. The plug-in noise elimination module adopts a modular design, so that quick disassembly, assembly and maintenance are facilitated; the anti-bird net enhances the outdoor adaptability of the equipment and prevents small animals from invading. By optimizing the structural design, the device has remarkable advantages in the aspects of reducing equipment noise, improving ventilation efficiency and facilitating maintenance, and is suitable for various scenes such as industrial, commercial energy storage and civil energy storage equipment.

A small animal imaging and radiotherapy (RT) irradiator device uses a circular array x-ray source to scan an anatomy of an animal, delivers conventional and ultra-high dose rate of radiation to the treatment target. The multipixel x-ray source includes a ring of focal spots capable of irradiating x-ray beams from around fifty beam angles. The x-ray beams are collimated with a collimator designed in treatment planning.

The utility model discloses a small animal sleep deprivation instrument for bioengineering, which belongs to the technical field of bioengineering and comprises a base, a bottom plate is arranged at the upper end of the base, a rotating shaft is arranged in the center of the bottom plate, and the lower end of the rotating shaft is connected with a driving motor in the base; a disturbance rod is arranged on the rotating shaft; a cylinder is mounted on the base, a top cover is arranged at the top end of the cylinder, and vent holes are formed in the top cover; an illuminating lamp is mounted on the bottom surface of the top cover or the side wall of the barrel; a water feeder and a feeder are arranged on the outer wall of the barrel; a touch screen is arranged on the front face of the base. The technical problem to be solved by the utility model is mainly how to improve the automation degree of small animal sleep deprivation experiments and the accuracy of data collection, reduce the pressure on animals, improve animal welfare and reduce the consumption of labor resources in the field of biomedical engineering.

The invention discloses a power distribution cabinet with a cabinet door having automatic closing and locking functions, and the power distribution cabinet comprises a cabinet body unit which comprises a cabinet body assembly, a transmission assembly disposed at the top of the cabinet body assembly, a door closing assembly disposed in the cabinet body assembly, a locking assembly disposed in the cabinet body assembly, and an unlocking assembly disposed in the cabinet body assembly. The limiting assembly is arranged in the cabinet body assembly; a maintainer can conveniently maintain the interior of the power distribution cabinet, after maintenance is completed, the maintainer only needs to lightly push the cabinet door and relieve limiting of the limiting assembly on the cabinet door, under the action of the door closing assembly, the door closing assembly drives the transmission assembly to rotate, the transmission assembly drives the cabinet door to rotate, and the door closing effect is achieved; meanwhile, after the cabinet door is closed, the cabinet door is locked under the action of the locking assembly, so that the cabinet door can be prevented from being opened for a long time, small animals can be prevented from entering the power distribution cabinet to damage the interior of the power distribution cabinet, and normal operation of the power distribution cabinet is guaranteed.

A specimen support system for supporting a small animal during pre-clinical imaging. The specimen support system comprises an animal bed for supporting the small animal, and a heating system linked to the animal bed. The heating system comprises a heat pad for influencing the temperature of the small animal when supported on the animal bed, the heat pad comprising a layer of graphite. The heating system is adapted for controlling an energy supplied to the layer of graphite thus influencing the temperature of the small animal.

The farmland drainage system comprises a hard drainage channel and an ecological purification section, the ecological purification section comprises a frame protection unit and an internal filling unit, a bottom liner layer and a partition layer are arranged in the hard drainage channel to form a filling lattice, and the filling lattice is filled with a first filling matrix and a second filling matrix. The first filling matrix can filter and purify the farmland drainage, intercept pollutants in the farmland drainage, slow down the drainage flow rate and prolong the hydraulic retention time; the second filling matrix is used for planting aquatic plants, inhabitation and reproduction spaces are provided for small animals such as frogs and other microorganisms and planktons, the ecological benefits of an original hard drainage channel are improved, and meanwhile the planted aquatic plants can absorb pollutants in water through root systems and cooperate with the microorganisms to purify water. The invention further provides a farmland hard drainage channel transformation method.

The invention provides a transformer substation cable trench small animal invasion identification method, which comprises the following steps of: installing a multi-gas sensing module in a cable trench to detect odor and generate gas data, uploading the gas data to a data processing terminal by using a data transmission module, receiving the gas data by the data processing terminal to perform preprocessing and feature extraction, and transmitting the gas data to a server; and then whether small animals exist in the cable trench is judged through the intelligent identification system according to the processed gas data, and a corresponding identification result is output. According to the intelligent identification system, a deep learning algorithm is adopted, network training is carried out through a large amount of sample data, and the identification accuracy and stability are improved. Meanwhile, operation and maintenance personnel can be reminded to take corresponding protection measures according to the identification result, intelligent safety protection of the cable trench is achieved, and the safety of the cable trench of the transformer substation can be improved.

The utility model relates to a pipeline water outlet structure, which belongs to the technical field of pipeline water outlets and comprises a round pipe and a water guide plate, the water guide plate is fixedly mounted at one end of the round pipe, a baffle is rotatably mounted at the lower end of the water guide plate, a spiral connecting plate is fixedly mounted on the inner side of the round pipe, a fixing plate is fixedly mounted on the outer side of the round pipe, and round holes are formed in two sides of one end of the fixing plate. Impact force generated by resetting of the baffle is buffered through the spring and the rubber buffer pad, the baffle is prevented from being damaged, the service life of the baffle is prolonged, small animals such as mice and squirrels on the ground can be blocked by arranging the baffle, and when the drainage pipe does not drain water, the small animals can be prevented from entering the drainage pipe along the pipe drainage port, and the drainage pipe is protected. And the rotating round pipe is connected with the telescopic pipe in a clamped mode through the spiral connecting plate, connection is convenient and fast, and installation is convenient.

The invention provides an animal invasion alarm method and device, electronic equipment and a readable storage medium. The method comprises the following steps: acquiring monitoring data of a target area; under the condition that it is determined that an animal exists in at least one of the first target area and the second target area according to the monitoring data, the hazard risk level of the animal is determined, and the hazard risk level is used for representing the possibility of causing harm to the target area after the animal invades; and determining a corresponding area alarm strategy according to the hazard risk level, and executing an alarm operation according to the area alarm strategy. According to the application, the target area can be comprehensively monitored, a monitoring blind area is avoided, small animal invasion can be found in the first time, measures can be taken in time, and the operation stability and safety of the target area are improved.

The utility model discloses a small animal operation fixing device, which belongs to the technical field of small animal treatment, and comprises a fixer, the fixer comprises a lower air bag cushion and an upper air bag cushion which are oppositely arranged up and down, and a plurality of communicated telescopic hoses are communicated between the lower air bag cushion and the upper air bag cushion. An inflation tube is installed on the upper air bag cushion in a penetrating mode, a monitoring sensor is installed on the surface of the upper air bag cushion, and a plurality of elastic connecting bands are fixedly connected to one side of the lower air bag cushion and one side of the upper air bag cushion; a lower air bag cushion and an upper air bag cushion are arranged to be communicated with a telescopic hose in a matched mode for use, limbs of a mouse penetrate through limb openings, and the lower air bag cushion and the upper air bag cushion are attached to and tightly wrap the chest and the back of the mouse respectively, so that the mouse can be fixed in the shape of a Chinese character'da ', the fixing effect is good, it can be guaranteed that surgery operation is conducted in the good position, and the operation convenience is improved; and the thoracic-abdominal opening and the back opening are utilized to facilitate acupuncture and massage treatment of the thoracic-abdominal part and the back part.

The present application discloses a novel substation anti-small animal thorn device, which is applied to the substation, comprising an intermediate steel plate, upper triangular teeth and lower triangular teeth: the intermediate steel plate is installed on the outer surface of the substation wall near the upper end; the upper triangular teeth are in several groups, one end of the upper triangular teeth is detachably connected to the side of the intermediate steel plate; the lower triangular teeth are in several groups, one end of the lower triangular teeth is detachably connected to the other side of the intermediate steel plate; wherein the upper triangular teeth and the lower triangular teeth are arranged at equal intervals; the technical key points are that the novel substation anti-small animal thorn device of the present invention can form two "thorny" walls, one long and one short, which can effectively prevent small animals from climbing the wall to enter the substation, and can prevent people with ulterior motives from entering the substation and posing a threat to the power grid, with good protection effect and good use prospects.

The invention discloses a method for constructing an atrial fibrillation small animal model through succinic acid induction and application, and the method comprises the following steps: in an animal model construction period, enabling a small animal to ingest an aqueous solution containing succinic acid or all pharmaceutically acceptable salts of succinic acid every day, and finishing the modeling period to obtain the animal model. Compared with a traditional angiotensin AngII administration method, the atrial fibrillation small animal model has the advantages that the cost of a medicine for constructing the animal model is lower, the economic benefit is higher, and the atrial fibrillation induction effect is better; the model represents the most common atrial fibrillation type in clinic, and compared with an acute transient atrial fibrillation model, the research and application range is wider; compared with tail vein injection, intraperitoneal injection, subcutaneous pump burying and other modes, the non-creative model has the advantages that damage to small animals is smaller, and the requirement for the operation technology is low.

The invention relates to a small animal detection device based on a holder double-camera technology and a detection method thereof. The device comprises a holder, a 3D camera, a zoom camera and an edge calculation controller, the holder at least has two rotational degrees of freedom; the 3D camera and the zoom camera are installed on the holder side by side. The edge calculation controller is respectively connected with the holder, the 3D camera and the zoom camera, so that the rotation angle of the holder can be controlled, and whether a small animal exists or not can be judged according to information acquired by the 3D camera and the zoom camera; according to the invention, rough scanning is carried out through the 3D camera, whether abnormity exists is rapidly judged according to the morphology difference value, and the abnormal position is amplified and captured through the zoom camera, so that the to-be-detected target has a large proportion in the picture, interference of light and shadow changes and the like on a common visible light camera is avoided, misjudgment is reduced, identification is facilitated, and the detection accuracy is improved. The system has the advantages of high efficiency, clear snapshot, high yield and the like, and meanwhile, the pan-tilt expands the scanning range and can cover a large range.

The invention discloses a laboratory animal behavior analysis method and system, and belongs to the technical field of animal behavior analys.The laboratory animal behavior analysis method includes the steps that A00, not less than three small animals are placed in a standard animal test box, and each small animal is dyed and distinguished; a01, the AI camera identifies and marks animal actions and postures and transmits marked video segments to the AI server; a02, the AI server carries out video reconstruction on the marked video segments to obtain 3D pictures, and repeated marks are removed; a03, the AI server counts the number of times of actions and postures of each animal. The AI camera carries out real-time edge processing on a shot video, animal postures and behaviors are recognized through an AI model library, and if it is judged that the postures and behaviors conform to actions and postures needing to be counted, relevant marks are synchronously marked in video stream signals; transmitting the marked video to an AI server; and the AI server performs screening statistics on the identification videos and gives a statistical result, so that the contrast experiment efficiency is improved.

The present invention discloses a rainwater collection and treatment device for a sponge city, comprising a top plate, a protective cover arranged on the top of the top plate, and a fixed frame fixedly connected to the bottom of the top plate, and the present invention relates to the field of urban rainwater treatment technology. The rainwater collection and treatment device for a sponge city adopts an integrated sinking ground drainage device, and the whole adopts a removable manner, so that the internal components can be easily replaced or impurities can be cleaned. When underground drainage is carried out on rainy days, the treatment boxes on both sides of the inside of the top plate are raised for use. When it is sunny and not raining, the top of the treatment box is flush with the top of the top plate to prevent mice or other small animals from entering the inside of the treatment box through the through groove, affecting the subsequent use of the treatment box. When it is raised, it is integrated with the scraper structure on the top of the top plate. The movement of the scraper drives the structure inside the treatment box to move, and the scraper pushes the debris on the top of the top plate to the through groove on the treatment box.

The invention relates to the technical field of magnetic resonance, in particular to a multi-nuclear magnetic resonance coil device for small animals and a magnetic resonance system.The multi-nuclear magnetic resonance coil device comprises a first coil assembly, a second coil assembly, a third coil assembly and a fourth coil assembly, the resonant frequencies of the first saddle-shaped coil and the first solenoid coil correspond to a first atomic nucleus; the second coil group comprises a second saddle-shaped coil and a second solenoid coil which extend on a second cylindrical surface, and the resonant frequencies of the second saddle-shaped coil and the second solenoid coil correspond to second atomic nucleuses different from the first atomic nucleuses; the second cylindrical surface is coaxially positioned on the inner circumferential side of the first cylindrical surface; and the examination cavity is configured on the inner peripheral side of the second cylindrical surface and is used for accommodating the small animal.

The invention discloses a small animal in-vivo multi-parameter dynamic monitoring method based on photoacoustic imaging, and relates to the technical field of biomedical imaging, and the method comprises the steps: obtaining a photoacoustic signal generated by a small animal under the excitation of pulse laser output by an optical excitation module through an acoustic detection module; reconstructing a photoacoustic image of the photoacoustic signal through a filtering back projection algorithm; calculating the similarity between adjacent images in the photoacoustic image, identifying the image of which the similarity is lower than a set frame threshold value as a cross section slice polluted by respiratory motion artifacts and removing the cross section slice, and replacing the removed image frame by using the interpolation of an adjacent artifact-free image so as to obtain an updated photoacoustic image; selecting a region of interest in the photoacoustic image, and carrying out oxygenation kinetics monitoring, pharmacokinetics monitoring, perfusion kinetics monitoring or nanoparticle in-vivo kinetics monitoring; according to the monitoring method, motion artifacts caused by breathing of the small animals are effectively inhibited, and in-vivo multi-parameter dynamics monitoring of the small animals is achieved.

The utility model provides an animal ear tag, relates to the technical field of positioning tags, and aims to solve the problems that an existing RFID tag suitable for a small animal is implanted under the skin of the animal in an intrusive injection mode, operation is inconvenient, and use limitation is large. Comprising an ear tag body and an induction box, the ear tag body comprises a first bent edge and a second bent edge which are arranged in pairs, and the first bent edge and the second bent edge both extend towards the middle line direction of the ear tag body; the induction box is provided with a first connecting hole and a second connecting hole which are arranged in pairs, the first connecting hole and the second connecting hole are located in the two opposite side faces of the induction box respectively, the positions of the first connecting hole and the second connecting hole correspond to the positions of the first bent edge and the second bent edge, the first bent edge is inserted into the first connecting hole, and the second bent edge is inserted into the second connecting hole. The second bent edge is inserted into the second connecting hole; the ear tag body is provided with an identification area. The device is convenient to operate, and has both naked eye readability and machine readability.

The invention relates to the technical field of animal experiments, and provides a small animal experiment operation platform which comprises a platform body, the platform body comprises an operation area used for placing small animals for experiment operation, vertical rods are evenly arranged on the bottom side of the operation area in a penetrating mode, and each vertical rod can independently ascend and descend. The operation platform further comprises a plurality of pressing pieces used for being arranged on the vertical rods in a sleeving mode to press and fix the small animals. After a small animal to be experimented is placed in the operation area, a fixing part is determined according to the actual size of the small animal body and the experimental design, then the vertical rods near the fixing part are sequentially selected and moved upwards by a certain distance, and then the vertical rods moving upwards are sequentially sleeved with pressing pieces matched with the fixing part. The vertical rods which are uniformly distributed in a dot-matrix manner are used as fixing anchor points, and then the pressing sheet is sleeved on the fixing anchor points for pressing and fixing, so that the fixing requirements of different body sizes and custom fixing parts are met, and the flexibility and the accuracy of small animal experiment operation are greatly improved.

A reusable snake trap assembly is an apparatus that can be used to effectively trap snakes and other unwanted critters for removal. The apparatus is designed to be continuously used to trap snakes and other critters in a safe and efficient manner. The apparatus includes a trap housing and an adhesive tray. The trap housing is a durable structure designed to attract snakes and critters by providing shelter from the weather. The trap housing is also designed to safely retain the adhesive tray to trap the snakes and critters that enter the trap housing. The adhesive tray is a disposable structure designed to trap the snakes and critters that enter the trap housing. This enables the apparatus to be used repeatedly by replacing the adhesive tray. The adhesive tray is also designed to be safely handled by the user while holding the trapped snakes and critters.

The invention belongs to the technical field of animal model construction, and particularly relates to a non-alcoholic fatty liver disease mouse model based on humanized PNPLA3 I148M transgene as well as a construction method and application thereof, the construction method comprises the following steps: step i: designing CDS of a human gene and a key 3 'UTR element for patent medicine of a small nucleic acid drug in gene plasmid DNA of the constructed model; step ii, using Luc2 or iRFP to report gene expression in the gene plasmid DNA for constructing the model; and (iii) a disease model of the fatty liver disease induced by combining plasmid DNA transient transfer with high-fat diet. The mouse model constructed by the invention has the advantages that a specific exogenous gene expression signal of the liver is detected in real time through small animal living imaging, a human PNPLA3 protein is detected and overexpressed through serum ELISA, PNPLA3 I148M mRNA is detected and overexpressed through liver RT-qPCR, the phenotype of the non-alcoholic fatty liver disease of the liver is determined through 15-week-old pathology, and the whole process of modeling for 8 weeks does not need to be operated in a biosafety secondary laboratory.

The invention belongs to the technical field of sampling and screening, and discloses a sampling and screening device for benthonic animals in a fresh water body, which comprises a working shell, one side of the working shell is communicated with a rectangular shell, the top of the working shell is fixedly connected with a traction rope, one side of the outer wall of the rectangular shell is fixedly connected with a flexible filter screen, and the sampling and screening device further comprises a sampling and screening mechanism, the sampling and screening mechanism comprises a square shell fixedly connected to the two ends of one side of the outer wall of the working shell, one side of the inner wall of the square shell is fixedly connected with a motor, the output end of the motor is fixedly connected with a rotating shaft, and the working shell also drives the rectangular shell and the flexible filter screen to move together. And on the flexible filter screen, small animals at the water bottom can be screened by a screen opening of the flexible filter screen and enter the working shell, so that the sampling of the animals is completed. After entering the working shell, the animals further enter the working shell through the sampling shell. Small animals and silt can be filtered and discharged through the filtering holes in the inner wall of the sampling shell, so that the collected objects are effectively screened.