The present invention discloses a glass cutting method, a two-dimensional code is set on a glass substrate before glass cutting, two-dimensional code information is read before the glass cutting, and inputted into a control system, the control system interprets the two-dimensional code information and generates a cutting path according to interpretation information, and a cutting device cuts the glass substrate according to the cutting path. According to the method, different sizes and different shapes of glass to be cut can be optimized and arranged, the utilization rate of the glass substrate can be improved, the workload of manual input of parameters can be reduced, the relative position of the two-dimensional code on the cut glass is more precision, production efficiency of the glass subsequent processing can be improved, mixed production of different specifications of glass can be achieved, positional deviation increase, large glass size scattering difference and other defects caused by a plurality of times of repeated positioning can be avoided, and flexible secondary processing of the glass can be facilitated. The present invention also discloses the glass cutting device for cutting the glass substrate by use of the method.