57 results about "Auto fluorescence" patented technology

The invention provides a construction method of a plant tissue space transcriptome sequencing library, which comprises the following steps: fixing and embedding plant tissues to obtain embedded blocks; performing autofluorescence detection and tissue permeabilization treatment on part of the embedded blocks, and determining an autofluorescence threshold value and target tissue permeabilization time; slicing, pasting and fixing the remaining embedding blocks, and performing microscope fluorescence scanning detection and tissue permeabilization treatment on the tissue-containing chip according to an autofluorescence threshold value and target tissue permeabilization time to obtain a permeabilized tissue chip; and carrying out reverse transcription, tissue removal, cDNA release, recovery and amplification on the permeabilized tissue chip to obtain a cDNA amplification product. The plant tissue transcript constructed by the construction method is not easy to diffuse and high in capture rate, high-quality in-situ capture time-space group data can be obtained, the accuracy and the credibility are high, the sequencing accuracy is ensured, and the application value is high.

Devices, systems, and methods are provided for imaging cholesteatoma, e.g., using an endoscope including a light source configured to deliver visible light at one or more narrowband wavelengths distally from the endoscope selected to cause autofluorescence in target tissue, a camera coupled to the imaging element to acquire image signals of locations beyond the distal end, and one or more filters configured to remove wavelengths of light in the image signals acquired by the camera including the one or more narrowband wavelengths of visible light.

This invention discloses a near-infrared long-afterglow nanocomposite, its preparation, and its application in uric acid detection, belonging to the technical field of testing or analyzing materials by measuring their chemical or physical properties. The nanocomposite comprises a near-infrared long-afterglow luminescent center and an outer layer of organosilicon-doped mesoporous silicon containing tetrasulfide bonds. Under near-infrared light excitation, this nanocomposite exhibits excellent afterglow luminescence enhancement response to hydrogen peroxide. Since the product of uric acid decomposition by uricase is hydrogen peroxide, this composite enables highly sensitive and rapid detection of uric acid in blood. The composite provided by this invention has uniform particle size and high stability, effectively eliminating autofluorescence interference from biological components and improving detection accuracy.

The application provides a chemical luminescence substrate with high chemical luminescence intensity, long wavelength and good stability, and a preparation method and application thereof. The chemical luminescence substrate has a structure shown in formula I. In addition, the application provides spontaneous fluorescence wavelength and intensity of the chemical luminescence probe in a physiological environment, and application of the chemical luminescence probe in in-vivo imaging. Test results show that the chemical luminescence probe provided in the application has long-wavelength spontaneous fluorescence (600 nm), can effectively penetrate skin tissue, has high spontaneous fluorescence intensity (> 10 7 p / s / cm 2 / sr), excellent thermal stability (without 1,2-dioxetane structure) and diversified detection groups (suitable for different detection models).

The present invention provides an integrated platform for exciting a molecular fluorescence signal, a detection chip, and an optical packaging structure. The integrated platform for exciting the molecular fluorescence signal comprises, arranged in sequence: an input optical coupling structure (1), a first filtering structure (2), an optical beam-splitter tree structure (3), a filtering structure array (4), a fluorescence excitation structure array (5), and an optical coupling-out structure array (6). The detection chip is used for detecting molecular fluorescence and comprises, arranged from bottom to top in sequence: a fluorescence detection layer (103'), a fluorescence collection layer (102'), and a fluorescence excitation layer (101'). The fluorescence excitation layer (101') comprises, arranged in sequence: an input optical coupling structure (1011'), an optical beam-splitter tree structure (1013'), a fluorescence excitation structure array (1014'), and an optical coupling-out structure array (1015'). The fluorescence excitation layer is further provided with an optical feedback structure (1012'), wherein an input end of the optical feedback structure (1012') is connected to a first output end of one branch of the optical beam-splitter tree structure (1013'). The optical packaging structure comprises an optical fiber module (2') and the detection chip (1'). The integrated platform in the present invention incorporates on-chip filters, providing an integrated fluorescence excitation solution capable of suppressing broadband background light arising from spontaneous fluorescence of lasers or waveguides, thereby improving a signal-to-noise ratio. The detection chip and the optical fiber module in the optical packaging structure in the present invention employ a passive optical coupling scheme, thereby facilitating stability of fluorescence signals and reducing complexity of external device optical paths.

A medical image processing device 9 includes an image processing unit 92 for processing pixel signals acquired from pixels of an imaging element 513. The pixel signal includes: a first pixel signal having an unnecessary light component containing autofluorescence generated by light from a member forming an optical path for observation when light emitted from the light source device propagates through the optical path for observation, and an observation target fluorescence component containing fluorescence generated by the member forming the optical path for observation when light emitted from the light source device propagates through the optical path for observation; the observation object fluorescence component is emitted from a substance contained in the observation object excited by light; and a second pixel signal having at least an unnecessary light component. The image processing unit 92 includes a signal correction unit 923 that generates a corrected pixel signal on the basis of the first and second pixel signals and a correction coefficient set on the basis of spectral characteristics of fluorescence to be observed.

The application discloses a micro wearable blood glucose monitoring device. The device comprises a first part and a second part which are connected in series to form a ring; the first part comprises an integrated Raman probe, the integrated Raman probe comprises two light sources for generating a line spot and a slit; the two light sources are arranged on the two sides of the slit respectively, and the slit emits excitation light to the skin tissue to generate a Raman signal, the slit is provided with a long-pass filter film for filtering Rayleigh scattering, other stray light and spontaneous fluorescence; the device further comprises a miniature spectrometer for performing light splitting and detection of the Raman signal; the second part comprises an information processing unit for driving control of the light source and the miniature spectrometer and micro blood glucose online analysis, a touch display screen for reading and displaying blood glucose values, and a battery module for supplying power to the integrated Raman probe, the information processing unit and the touch display screen; the watchband is provided with a data line for transmitting data and a power line for power supply.

Translational rapid ultraviolet-excited sectioning tomography (TRUST) applies ultraviolet (UV) excitation to a sample and images fluorescence and autofluorescence emission for tomographically imaging the sample. Deep-learning neural networks are used to achieve higher imaging speed and imaging resolution. In one use, fluorescence images acquired with relatively low imaging resolution can be transformed into high-resolution images through the first conditional generative adversarial network (cGAN), a super-resolution neural network (e.g., ESRGAN), which is also helpful for reducing the image scanning time. In another use, the second cGAN, such as Pix2Pix, is used to realize virtual optical sectioning to enhance the axial resolution of the imaging system. Compared to the conventional pattern illumination methods (e.g., HiLo microscopy), which need at least two shots for each field of view, the imaging speed is also times higher because only one shot under the uniform-illumination condition of UV irradiation is required.

An optical imaging system according to an embodiment disclosed in the present document may include: a laser module configured to radiate an ultrashort pulse laser beam; a microscope module configured to transmit the radiated ultrashort pulse laser beam to an eye of a subject and detect reflected light from the eye or (auto)fluorescence in the eye using an image sensor; a control module configured to generate a tau-related image in which a modification of tau proteins is detectable from the reflected light detected through the image sensor; and an output device configured to display the tau-related image.

A device for detecting bacteria in a sample comprises an optical block (4) arranged to produce multispectral image sets of portions of a sample, obtained by illumination eliciting an autofluorescence response and by illumination eliciting a reflectance response. These multispectral image sets are provided to an analyzer (6) comprising a classifier that produces an image in which each pixel is derived from an identical pixel in the images of the multispectral image set and is associated with an element identifier indicating whether that pixel is associated with a bacterium or another element. This image is then used by a computer to determine bacteria detection data. Fig. 1

The invention relates to a device for detecting bacteria in a sample, the device comprising an optical unit (4) arranged to produce sets of multispectral images of portions of a sample, these being obtained by means of illumination eliciting an autofluorescence response and by means of illumination eliciting a reflectance response. These sets of multispectral images are fed to an analyser (6) comprising a classifier that produces an image, each pixel of which is drawn from an identical pixel in the images of the set of multispectral images and is associated with an element identifier indicating whether this pixel is associated with a bacterium or with another element. This image is then used by a computer to determine bacteria detection data.

The invention provides a dyeing method for removing autofluorescence of a single cell sorting micro-fluidic chip and a chip without autofluorescence. The dyeing method comprises the following steps: completely soaking the single-cell sorting micro-fluidic chip in a dye solution for dyeing until the top layer of the chip is dyed into a non-transparent black top layer, thereby obtaining the single-cell sorting micro-fluidic chip without autofluorescence, the dye in the dye solution is black dye; the single cell sorting micro-fluidic chip at least comprises a photosensitive dry film serving as a runner layer. The staining method provided by the technical scheme of the invention can effectively solve the problem of autofluorescence of a single cell sorting micro-fluidic chip prepared from a traditional photosensitive dry film, so that when the stained chip is applied to fluorescence activated cell sorting, recognition of automatically screened fluorescent cells is facilitated, the operation difficulty of fluorescence activated cell sorting is reduced, and the working efficiency is improved. And accuracy and reliability are improved.

A method and system for assessing a histopathological cause of kidney graft dysfunction in a subject following kidney transplantation is disclosed. The method comprises the steps of: a) generating one or more images of the native fluorescence emission from one or more exfoliated kidney cells obtained from a urine sample from the subject in a plurality of distinctive spectral channels, b) calculating, for each urinary exfoliated kidney cell, quantitative features of the autofluorescence signals in said one or more images, c) applying a feature selection methodology to the quantitative features to generate a set of optimised quantitative features; and d) applying a classification method to the optimised quantitative features to produce a multispectral profile for differentiating the histopathological cause of kidney graft dysfunction in the subject from a plurality of histopathological causes of kidney graft dysfunction.

The invention belongs to the field of fluorescent probes, and discloses a selenium cyano fluorescent probe for high-selectivity recognition of H2S as well as a preparation method and application of the selenium cyano fluorescent probe. The probe adopts dicyanoisophorone as a fluorophore skeleton and-SeCN group as a novel recognition site, and the structural formula is shown in the specification. The dicyanoisophorone has relatively large Stokes shift during release, so that the interference of autofluorescence on signal detection is reduced, and the dicyanoisophorone is connected with a-SeCN group through a condensation reaction. On the basis of a double nucleophilic substitution strategy, H2S induces conversion of-SeCN into-Se-S, and near-infrared fluorophores are released through intramolecular self-cyclization. Researches show that the probe has high sensitivity, selectivity, good water solubility and thiol consumption resistance, the detection limit is as low as 20 nM, and the probe has good ratio signal and brain targeting ability.

This invention discloses a short-wave infrared fluorescence endoscopic imaging device and method. Based on a pre-made near-infrared endoscope, the device achieves dual-path multiplexing of the original imaging and illumination channels. Specifically, the imaging channel performs white light imaging and excitation light scanning, while the illumination channel performs white light illumination and short-wave infrared fluorescence collection. The excitation light source module is connected to the scanning module, and the scanning and white light imaging modules are connected to the endoscope via a dichroic mirror. The short-wave infrared detection module and white light illumination module are connected to the endoscope via a multi-integrated fiber bundle. The control and data acquisition module is electrically connected to the scanning module, short-wave infrared detection module, and white light imaging module, respectively. This invention eliminates the need to modify the endoscope's structure, overcomes the technical bottleneck of pre-made endoscopes being unable to perform short-wave infrared fluorescence imaging, and achieves deep fusion of white light and short-wave infrared fluorescence dual-modal imaging. This effectively reduces scattering and autofluorescence interference, and improves the tissue penetration depth and signal-to-background ratio of endoscopic imaging.

An information processing device according to an aspect of the present disclosure includes a simulated image generation unit (131a) that generates a simulated image by superimposing a non-stained image including an autofluorescence component and a dye tile image in which a reference spectrum of a first fluorescent dye and imaging noise of each of pixels of the non-stained image are associated, a fluorescence separation unit (131b) that separates a component of the first fluorescent dye and the autofluorescence component on the basis of the simulated image and generates a separated image, and an evaluation unit (131c) that evaluates a degree of separation of the separated image.

The application belongs to the technical field of nano singlet oxygen fluorescent probe, and particularly relates to a near-infrared ratio type nano singlet oxygen fluorescent probe based on 2-pyridone and a preparation method and application thereof. The application upgrades the fluorophore to perylene monimide with a larger conjugated system and stronger push-pull electron ability, successfully red-shifts the emission spectrum to the near-infrared region / red light region (625 nm blue shift to 550 nm), perfectly avoids the interference of spontaneous fluorescence of biological tissues, and greatly improves the light penetration depth. The strong hydrophobic PMI-t-PY small molecule is nano-wrapped and self-assembled by using the amphiphilic liposome material (mPEG-DSPE) innovatively, directly solving the defects of poor water solubility and easy aggregation and fluorescence quenching in water of the traditional small molecule probe. The probe has high optical stability and specific selectivity in a wide pH range, and can realize high-fidelity, quantitative and dynamic visualization monitoring of endogenous singlet oxygen in living cells and photodynamic therapy.

Methods and systems described herein detect autofluorescence of a sample. These methods and systems obviate the need for addition of fluorophores to samples to create IR absorption. An IR source and an excitation source are used to generate emission spectra of the chosen sample, which are then analyzed to determine various data about the sample.

A method of analyzing a tissue is provided that includes: administering a fluorescent agent to a tissue; producing a first excitation light that is configured to produce a fluorescence emission from the fluorescent agent administered to the tissue; producing a second excitation light that is configured to produce an autofluorescence emission from a biomolecule of interest present within the tissue; using a photodetector to detect the fluorescence emissions and produce first signals representative thereof, and produce second signals representative of the autofluorescence emission; producing an image using the first and second signals. The image includes a first, second and third portions representative of tissue types present within the tissue; and analyzing the tissue to identify diseased tissue and to distinguish it from the first and second tissue types.

This invention relates to the field of biodetection technology, specifically disclosing a multiplex fluorescent staining kit for tissue samples. The kit includes an antibody dissociation buffer, a signal amplification deposition solution, a fluorescent labeling reagent, a tissue-protective imaging medium, and an autofluorescence background inhibitor. This invention utilizes a non-destructive elution technique achieved through the synergistic effect of a low-pH buffer and a directional electric field. It leverages the positively charged nature of antibodies under acidic conditions to drive them away from the tissue and towards the negative electrode, achieving gentle and efficient antibody removal. Simultaneously, it employs a two-step cascade amplification strategy of small molecule deposition and copper-free click chemical labeling, balancing deep penetration with ultra-high sensitivity. This invention can stably support 6-12 cycles of staining, exhibits high elution efficiency, preserves tissue morphology, and can detect low-abundance targets. It is suitable for spatial analysis of the tumor microenvironment, monitoring of transplant immune rejection, and pathological evaluation of neurodegenerative diseases, demonstrating promising clinical application prospects.

A device for measuring intrinsic autofluorescence of molecules present in a biological sample wherein the device includes: a) a light-sheet, virtual light-sheet or light line delivery pathway module including a laser generator capable of generating ultrashort pulses with a duration between 1 microsecond and 1 attosecond, and optical elements shaping the laser light into a light sheet, virtual light-sheet or light-line; b) an imaging chamber including an imaging dish inside of which the biological sample is placed, a translational stage, an illumination objective and a collection objective both immersed in an immersion liquid in a sealed immersion chamber, wherein both objectives are positioned below the imaging dish, wherein the immersion chamber is placed inside an incubation chamber, allowing environmental control; and c) a fluorescence detection pathway including optical elements, light-splitting elements such as diffractive elements or dichroic mirrors, an array detector, and a device control and signal processing unit.

The invention discloses a tissue sample processing method for three-dimensional pathological imaging, and belongs to the technical field of tissue sample processing. The tissue sample treatment method comprises the following steps: step a, decolorizing a tissue sample; b, tissue transparentizing based on the swelling hydrogel; in the step a, the discoloring of the tissue sample adopts a mode of matching physical discoloring with chemical discoloring; wherein the physical decolorization is realized by continuously irradiating a tissue sample under blue-violet light. The problem that autofluorescence of endogenous pigment and residual blood in a tissue sample interferes with biomarker observation is solved in a mode of matching physical decoloration and chemical decoloration, meanwhile, tissue transparentization is realized by utilizing expanded hydrogel, and the tissue form is kept stable, so that a good foundation is laid for subsequently realizing high-efficiency elution and multi-round marking of an antibody; therefore, in-situ multiple marking and high-resolution three-dimensional imaging of tissue samples, especially thick slices such as brain tumor tissues and the like can be realized.

Autofluorescence is the inherent emission of light from cells or tissues that occurs when biological samples are exposed to different wavelengths of excitation light. Tissue autofluorescence is usually ascribed to biological components such as flavins, porphyrins, chlorophyll (in plants), collagen, elastin, red blood cells (RBCs), and lipofuscin. Tissue autofluorescence often presents a significant problem in microscopy, pathology, and surgical applications. Autofluorescence interferes and complicates signal detection in a variety of fluorescence microscopy methods. Tissue autofluorescence reduces the signal detection sensitivity, and in some cases may cause failure in the detection of fluorescent dye signals. Accordingly, there is a need for further improved methods to decrease the autofluorescence intensity from microscopy samples both in research and clinical venues. Described herein is a photobleaching device utilizing a light source tuned to the full spectrum of light to quench autofluorescence of a biological sample.

The invention discloses a noninvasive AGEs concentration monitoring method and system, a storage medium and a watch. The method comprises the steps that excitation light sources with different wavelengths are adopted to excite fluorescence in skin tissue; obtaining the light intensity of autofluorescence under different wavelengths; the AGEs concentration is obtained according to discrete three-dimensional fluorescence spectrums established according to different excitation wavelengths. By means of the non-invasive characteristic, the comfort level and convenience of monitoring are remarkably improved, and a user can continuously monitor the health condition without interference in daily activities. The combination of an ultra-sensitive optical sensor and an intelligent algorithm not only ensures the accuracy and real-time performance of data, but also provides a reliable basis for early diagnosis and timely intervention.

The invention discloses a large-volume sample fluorescence labeling method based on tissue fixation. The fluorescence labeling method comprises the following steps: a sample fixing step: fixing a biological tissue sample by adopting paraformaldehyde; and a fluorescence labeling step: placing the biological tissue sample subjected to fixation treatment in a stationary liquid containing glyoxal, and realizing fluorescence labeling of the biological tissue sample by utilizing autofluorescence generated by browning due to fixation of glyoxal. According to the fluorescent labeling method, fluorescent dye and antibody labeling are not needed, labeling of cell bodies and fiber bundles can be achieved in a non-degreasing state, and meanwhile blood vessel information can be obtained through a contrast means; moreover, the method is easy to process, the sample can be rapidly and uniformly permeated, the sample processing time is greatly shortened, and a new way is opened up for obtaining three-dimensional structure information of different large sample tissues and organs at the submicron scale.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

Techniques for fluorescence imaging are provided. A tissue region comprising a target fluorophore is illuminated with an excitation light in a wavelength range of 380-490 nm. First image data of the tissue is captured at an image sensor, wherein the first image comprises an autofluorescence contribution from the tissue and a fluorescence emission contribution from the target fluorophore, wherein the first image data comprises red channel data and green channel data. A corrected fluorescence image is generated, based on the first image data, wherein generating the fluorescence image comprises subtracting a second component based on one of the green channel data or the red channel data from a first component based on the other of the green channel data or the red channel data.

The application belongs to the field of biomedical materials, and provides preparation and application of a near-infrared two-region luminescent material with AIE property, and the structure is: wherein Ar is a group with AIE property such as triphenylamine and carbazole derivative, X is hydrogen element, fluorine element and chlorine element, etc. The near-infrared luminescent material has AIE property, is simple to synthesize, has high near-infrared luminescent efficiency, adjustable absorption and emission spectrum, good light stability, and can overcome the interference of biological tissue self-absorption and spontaneous fluorescence and the like.

Techniques for fluorescence imaging are provided. A tissue region comprising a target fluorophore is illuminated with an excitation light in a wavelength range of 380-490 nm. First image data of the tissue is captured at an image sensor, wherein the first image comprises an autofluorescence contribution from the tissue and a fluorescence emission contribution from the target fluorophore, wherein the first image data comprises red channel data and green channel data. A corrected fluorescence image is generated, based on the first image data, wherein generating the fluorescence image comprises subtracting a second component based on one of the green channel data or the red channel data from a first component based on the other of the green channel data or the red channel data.

Examples of assessing susceptibility of a target pathogen based on autofluorescence, are described. In an example, a sample containing a target pathogen and a predefined concentration of a target drug may be obtained. Thereafter, autofluorescence features based on autofluorescence exhibited by the sample in response to the sample being subjected to excitation radiation, may be determined. Once the autofluorescence features are determined, they may be analyzed based on a susceptibility-detection model to determine susceptibility of the target pathogen with respect to the target drug. In an example, the susceptibility-detection model is trained based on a training autofluorescence features correlated with reference data, in which the reference data includes information pertaining to susceptibility or resistance of a reference pathogen with respect to a reference drug.