40 results about "Optical sectioning" patented technology

A multifocal scanning microscopy (MSM) for super-resolution imaging was developed with multicolor acquisition and minimal instrumental complexity. MSM implements a stationary, interposed multi-focal multicolor excitation by exploiting the motion of the specimens, realizing super-resolution microscopy through a general epi-fluorescence platform without compromising the image-scanning mechanism or inducing complex instrument alignment. The system is demonstrated with various phantom and biological specimens, and the results present effective resolution doubling, optical sectioning, and contrast enhancement. MSM, as a highly accessible and compatible super-resolution technique, may offer a promising methodological pathway for broad cell biological discoveries.

A surface shape measuring device measures the three-dimensional shape of a measurement surface in an object to be measured shaped as a rotating body or an approximate rotating body. The surface shape measuring device includes: a rotary table that rotates the object to be measured; an encoder that sequentially outputs signals according to the rotation angle of the rotary table; an optical sectioning sensor that irradiates light onto the measurement surface and acquires a plurality of optical section line image data by sequentially capturing optical section lines generated by the irradiated light, which move across the measurement surface as the rotary table rotates, triggered by a signal output from the encoder; and an image processing unit that generates an image showing the surface shape of the measurement surface by sequentially arranging the respective optical section line image data according to the corresponding rotation angle.

The invention belongs to the technical field of combination of biomedical engineering and intelligent monitoring, and discloses a vibration cutting tool wear state monitoring method suitable for biological soft tissue, which comprises the following steps: S1, executing vibration cutting on the biological soft tissue, and obtaining a three-channel cutting force original signal; s2, inputting the three-channel cutting force original signal into a one-dimensional residual network, and expanding the three-channel cutting force original signal into a multi-feature channel; s3, a channel attention mechanism is applied, and first enhancement is achieved for some feature channels with wear sensitivity; s4, continuing to apply the time attention mechanism, and focusing for the key time interval; s5, regression is carried out to obtain a corresponding cutter flank abrasion loss prediction value; and S6, the real-time state of the cutter is judged. Compared with the prior art, the method has the advantages that the monitoring precision and robustness in a soft tissue cutting application scene can be remarkably improved, and the high quality of subsequent microscopic optical section tomography combined with cutting, the continuity of three-dimensional modeling and the data reliability are correspondingly and effectively guaranteed.

A method for detecting an AFB1 content using fluorescence analysis based on optical sectioning is provided, which relates to the field of biological detection technologies. The method includes steps for detecting the AFB1 content by using a fluorescence probe combination, which includes fluorescence probes aptamer and PC-strand. The nucleotide sequence of the aptamer is shown as SEQ ID NO: 1, and a FAM group is labeled at a 3′ end of the nucleotide sequence shown as SEQ ID NO: 1. The nucleotide sequence of the PC-strand is shown as SEQ ID NO: 2, a TAMRA group is labeled at a 5′ end of the nucleotide sequence shown as SEQ ID NO: 2, and a PC group is labeled between 10th bp and 11th bp of the nucleotide sequence shown as SEQ ID NO: 2. The method can achieve photosensitive detection of AFB1, with high detection sensitivity and strong specificity.

An ophthalmic device and an ophthalmic device control program are provided that are capable of appropriately acquiring three-dimensional information about the anterior segment of the eye while stabilizing the fixation of the subject's eye. [Solution] The fixation target presenting optical system presents a fixation target to the subject's eye. The cross-sectional imaging optical system captures cross-sectional images of the anterior segment by receiving return light from the anterior segment that has been optically sectioned by the imaging light. The moving unit moves an optical unit including the fixation target presenting optical system and the cross-sectional imaging optical system relative to the subject's eye. The control unit can perform a three-dimensional information acquisition process to acquire three-dimensional information about the anterior segment by capturing cross-sectional images of multiple locations on the anterior segment while moving the optical unit and scanning the imaging light. When performing the three-dimensional information acquisition process, the control unit positions the fixation target within a limited area centered on the focal position of the entire lens system of the fixation target presenting optical system, regardless of the refractive power of the subject's eye.

The invention discloses an optical system for high-speed reduction of a dynamic deformation process of a cornea, which realizes high-precision analysis of physical characteristics of the cornea through a high-speed imaging technology, performs error compensation on the physical characteristics and realizes high-precision calculation of intraocular pressure. According to the technical scheme, the optical system comprises a slit lamp light source used for forming a cornea optical slice and ensuring the definition and the contrast ratio of a cornea slice image; the Samm lens is used for shooting a cornea optical section, inclined imaging light path design is adopted, and the depth of field meets the requirement that the cornea section keeps clear imaging in the dynamic process; and the high-speed camera is positioned behind the Chemma lens and is used for receiving the cornea optical slice image imaged by the Chemma lens so as to record the dynamic deformation process of the cornea.

A method for detecting an AFB1 content using fluorescence analysis based on optical sectioning is provided, which relates to the field of biological detection technologies. The method includes steps for detecting the AFB1 content by using a fluorescence probe combination, which includes fluorescence probes aptamer and PC-strand. The nucleotide sequence of the aptamer is shown as SEQ ID NO: 1, and a FAM group is labeled at a 3′ end of the nucleotide sequence shown as SEQ ID NO: 1. The nucleotide sequence of the PC-strand is shown as SEQ ID NO: 2, a TAMRA group is labeled at a 5′ end of the nucleotide sequence shown as SEQ ID NO: 2, and a PC group is labeled between 10th bp and 11th bp of the nucleotide sequence shown as SEQ ID NO: 2. The method can achieve photosensitive detection of AFB1, with high detection sensitivity and strong specificity.

An imaging system for imaging a biological sample or another sample containing fluorescent molecules may include an optical system with a light source emitting light, wherein the light is directed by the optical system to the sample via at least one plane being conjugate to the image plane. The optical source may have an extended radiation pattern, in other words, the radiation beam may have an extent in the x- and y- planes, rather than a point source. The extent of the illumination region in x and y may be based on and matched to the detector area onto which the radiation may be imaged, preferably a TDI detector. This novel system may include genomics, proteomics and transcriptomics work flows.

To enable quick and accurate measurement of the surface shape of an object to be measured of a so-called rotating body or an approximate rotating body. A surface shape measuring device according to the present invention measures the three-dimensional shape of a measurement surface in an object to be measured in the shape of a rotating body or an approximate rotating body by optical sectioning method. The surface shape measuring device comprises: a rotary table that rotates the placed object to be measured in a circumferential direction; an encoder that sequentially outputs signals according to the rotation angle of the rotary table; an optical sectioning sensor that irradiates a band of light or a line of light onto the measurement surface and acquires a plurality of optical section line image data by sequentially capturing optical section lines generated by the band of light or the line of light, which move across the measurement surface as the rotary table rotates, triggered by a signal output from the encoder; and an image processing unit that generates an image showing the surface shape of the measurement surface by sequentially arranging the respective optical section line image data according to the corresponding rotation angle.

The invention relates to the technical field of oral cavity imaging, in particular to an intelligent electronic tongue depressor and a high-precision three-dimensional oral cavity imaging method. According to the structural features of different frames of optical slice images, the local deformation possibility of each matching feature point in each frame of optical slice image is obtained, and matching error feature points forming a deformation target area are screened out; for any frame of optical slice image, according to the local deformation possibility of different matching feature points in the deformation target area, the positions of the different matching feature points in the three-dimensional point cloud model and the change trend of a corresponding moving path when the tongue depressor intersects with the deformation target area, the matching error consistency of the deformation target area on each frame of optical slice image is obtained; and in combination with the point cloud coordinates of the matching error feature points in the deformation target area and the position distribution of the deformation gravity center, obtaining point cloud correction coordinates of the matching error feature points. According to the method, the point cloud correction coordinates of each image deformation area are accurately obtained, and the accuracy of oral cavity three-dimensional model construction is improved.

The present invention discloses a method for observing the reaction sites of crosslinked starch, including the steps of preparation of crosslinked starch, silver nitrate staining, centrifugation, washing of the dye, centrifugation, UV reduction, preparation of a suspension of stained starch granules, and observation with a laser confocal microscope. Specifically, STMP crosslinked starch is prepared by a semi-dry method or POCl3 crosslinked starch is prepared by a wet method; silver nitrate is used to convert the anions of starch granules into silver salts; the silver ions of the silver-exchanged starch are reduced to silver atoms (Ag + →Ag ° ) by ultraviolet light; the sample is observed by optical sectioning with a laser confocal microscope, and the distribution of reaction sites in the starch granules is obtained by excitation and in-situ scanning imaging. The present invention provides a simple, effective, and direct method for observing the distribution of reaction sites of crosslinked starch.

The application discloses a multispectral imaging system with deep ultraviolet oblique optical slicing capability, comprising a core lens assembly, a coaxial reflector and a guide ring-shaped illumination system; the core lens assembly comprises one or more lens elements; the coaxial reflector is located at the top of the core lens assembly and is arranged around the core lens assembly; the implementation scheme of the guide ring-shaped illumination system comprises a light source and a hollow reflecting surface, a ring-shaped light source and a ring-shaped light guide, the position of the light guide is adapted to the position of the core lens assembly, the position between the light source and the light guide is adapted, oblique illumination of the illumination light including deep ultraviolet is realized by adding the coaxial reflecting surface around the core lens for close-range focusing, and thus oblique illumination is realized, and the coaxial oblique illumination design overcomes the physical limitation of external off-axis light sources and is compatible with high-NA lenses.

Translational rapid ultraviolet-excited sectioning tomography (TRUST) applies ultraviolet (UV) excitation to a sample and images fluorescence and autofluorescence emission for tomographically imaging the sample. Deep-learning neural networks are used to achieve higher imaging speed and imaging resolution. In one use, fluorescence images acquired with relatively low imaging resolution can be transformed into high-resolution images through the first conditional generative adversarial network (cGAN), a super-resolution neural network (e.g., ESRGAN), which is also helpful for reducing the image scanning time. In another use, the second cGAN, such as Pix2Pix, is used to realize virtual optical sectioning to enhance the axial resolution of the imaging system. Compared to the conventional pattern illumination methods (e.g., HiLo microscopy), which need at least two shots for each field of view, the imaging speed is also times higher because only one shot under the uniform-illumination condition of UV irradiation is required.

The invention discloses a three-dimensional compressed sensing reconstruction method based on reset scanning and joint prior. Comprising the following steps: sequentially performing forward scanning and reset scanning by using a compressed sensing three-dimensional imaging system to obtain a compressed frame image stack and an additional frame image stack of a sample, respectively performing pre-reconstruction by using an alternating multiplier method to obtain a compressed frame pre-reconstruction image stack and an additional frame pre-reconstruction image stack, and processing by using a cross-correlation method to obtain a dislocation amount of a reset process; and establishing a joint reconstruction enhanced compressed sensing three-dimensional reconstruction model according to the dislocation amount in the resetting process, and inputting a compressed frame and an additional frame image stack to obtain a reconstructed three-dimensional image. The method disclosed by the invention has good low cost, practicability and acquisition efficiency, the availability of the compressed sensing algorithm on a three-dimensional tissue imaging system such as a light sheet microscope is improved, and a new scheme is provided for low-cost and high-efficiency three-dimensional optical tomography microscopic imaging.

The present invention belongs to the field of micro-nano optical technology, and relates to a wavelength multiplexing metasurface and design method for snapshot HiLo optical sectioning microscopic imaging, comprising the following steps: Step 1: Determine the relationship curves between the transmission phase and the transmission phase difference under the two working wavelengths of uniform illumination light and structured illumination light and the structural parameters of dielectric nanocolumns, and establish a basic unit structure phase library; Step 2: Determine the target phase difference between the uniform illumination light and the structured illumination light; Step 3: Screen the dielectric nanocolumns with equal target phase difference under the two working wavelengths in the basic structure unit library; Arrange the two screened dielectric nanocolumns according to the designed phase distribution equation to obtain a wavelength multiplexing metasurface structure. Compared with conventional HiLo microscopic imaging technology, the present invention effectively improves the imaging speed and imaging stability, reduces the complexity of the imaging and reconstruction process, and provides a new idea for snapshot HiLo microscopic imaging.

Describes systems and methods for providing quantitative measurements of the distribution of a contrast agent in a tissue sample by identifying the cellular distribution of the contrast agent using a combination of high-resolution optical sectioning imaging modalities. In some cases, the systems and methods can be used in an intraoperative setting to guide biopsies or surgeries.

This invention belongs to the technical field of surface morphology measurement, and discloses a method and apparatus for measuring morphology based on pixel-by-pixel high dynamic structured illumination. The method includes: S1: calibrating the DMD and camera to obtain the grayscale response relationship and coordinate mapping relationship between the DMD and the camera; S2: correcting the phase-shifting fringes generated by the DMD using the grayscale response relationship, coordinate mapping relationship, and optical transfer function; S3: projecting the corrected phase-shifting fringes onto the sample surface and acquiring a phase-shifted structured illumination image by the camera; S4: performing slicing imaging on the phase-shifted structured illumination image to extract the three-dimensional morphology of the sample surface. This application can improve the imaging quality of the camera pixel by pixel, and then obtain the three-dimensional morphology of the sample surface by optical slicing multiple high-quality structured illumination images.

The invention relates to the technical field of optical imaging, and provides a wide-field optical section imaging method based on virtual illumination modulation, and the method comprises the steps: obtaining an initial wide-field illumination image and a modulation stripe image of the initial wide-field illumination image, and carrying out the virtual illumination modulation according to the modulation stripe image and the initial wide-field illumination image, obtaining a structural illumination image corresponding to the initial wide-field illumination image, obtaining high-frequency information and low-frequency information corresponding to the initial wide-field illumination image according to the initial wide-field illumination image and the structural illumination image, and obtaining the wide-field illumination image based on weighted fusion of the high-frequency information and the low-frequency information. And obtaining a wide-field optical section tomographic image corresponding to the initial wide-field illumination image. By means of the mode, use of an optical modulation device is avoided, the wide-field optical section tomography effect is achieved only through one initial wide-field illumination image, the wide-field optical section imaging efficiency is improved, the problem that a traditional optical section tomography technology is affected by tissue scattering is solved, and the wide-field optical section imaging quality is improved.

The invention discloses a method for simultaneously observing the distribution of starch granule-bound proteins and lipids, including the steps of starch protein staining, starch lipid staining, centrifugation, cleaning, preparation of a double-stained starch granule suspension, and laser confocal microscopy observation, wherein the method is characterized in that: starch granule proteins and lipids are stained respectively with fluorescamine and Pro-Q Diamond, thereby fluorescently labeling proteins and lipids in starch granules at the same time; optically sectioning the sample with a laser confocal microscope, and obtaining the distribution of proteins and lipids in starch granules by dual-channel excitation and in-situ scanning imaging. The invention solves the problem that the distribution of starch granule-bound proteins and lipids cannot be observed simultaneously.

Histology and histopathology relate to the study of cells and tissues under microscopy to diagnose and monitor diseases, such as cancer. Differential staining and imaging of various (10) structures in tissue can provide detailed information at cellular and subcellular levels, which is essential in tissue analysis, including diagnostic and prognostic evaluations. The present invention relates to systems and methods for tissue treatment and imaging, including simultaneous dehydration and staining with a fluorescent nuclear dye and a fluorescent protein dye followed by optical slice microscopy.

A 3D single-molecule super-resolution imaging technique is disclosed herein. In particular, the techniques described herein combine an optical platform including a tilted single-objective dithered light sheet illumination, a microfluidic system, and point spread function engineering to achieve precise 3D single-molecule super-resolution imaging of subcellular structures (e.g., a sample (110)) with easy control of the extracellular environment. In certain embodiments, the single-objective dithered light sheet (102) of the optical setup (100) includes a plurality of galvanometric mirrors (116, 118, 124) and a tunable lens (122) that may steer a light sheet (102) for optimized optical sectioning of the sample (110). In some embodiments, the sample (110) is positioned in the microfluidic device (160) during imaging. In this way, traditional challenges (e.g., high out-of-focus background fluorescence and lack of environmental control) generally facing single-molecule localization microscopy may be overcome.

The invention relates to a single viewpoint imaging system for optically sectioning an object from a single viewpoint, comprising: (a) a TPNDBs (tilted pseudo-nondiffracting beams)-type coded phase mask (CPM) configured to receive a light beam passed through the object or reflected therefrom, and to produce TPNDBs directed towards a sensing array; (b) said sensing array configured to record an image formed by said TPNDBs impinged thereon; (c) a processor configured to: (c1) separately cross-correlating said recorded image with at least one point-spread function (PSF) previously acquired or calculated at the same system with the same CPM, each said PSF reflects a point object positioned at one specific longitudinal distance, respectively, from said array; (c2) storing the results of said separate cross-correlations, each such cross-correlation result relates to an image of another section, respectively, of the object; and (c3) uniting all said cross-correlation results to reconstruct a final image of the object.

A nondestructive positioning and imaging method based on a double-helix point spread function (DH-PSF) comprises: utilizing the DH-PSF to phase modulate a target beam transmitted through a scattering medium; performing scanning detection by varying the target's axial defocus, thereby acquiring a plurality of modulated images; evaluating the DH-PSF's positioning capability in a scattering environment using a signal-to-background ratio; calculating the target's three-dimensional position based on the series of modulated images; and performing a deconvolution optical sectioning algorithm on the series of modulated images using a system focal plane (DH-PSF) to obtain a clear structure of the target and the scattering medium, thereby reconstructing the target's three-dimensional distribution within the scattering medium. This method utilizes the DH-PSF's positioning capability in scattering environments and designs a corresponding algorithm based on its high recognition capability to reconstruct a three-dimensional image of the target within the scattering medium, achieving high resolution and a large penetration depth in situ and even within living tissue.

According to one aspect, the present description relates to a device for analysing a wavefront, configured to be connected to a fluorescence microscopy imaging system with optical sectioning, equipped with a microscope objective comprising a pupil in a pupil plane, the analysis device comprising a two-dimensional detector comprising a detection plane; a two-dimensional arrangement of microlenses, arranged in an analysis plane, each microlens being configured to form, on the detection plane, when the analysis device is connected to the microscopic imaging system, an image of an object situated in a focal plane of the microscope objective, with a given analysis field; an optical relay system configured to optically conjugate the analysis plane and the pupil plane; a field diaphragm positioned in a plane optically conjugated with the plane of detection, and configured to define said analysis field; a processing unit configured to determine, based on the set of images formed by the microlenses, a two-dimensional map of a characteristic parameter of the wavefront in said analysis plane.

The invention relates to the technical field of image reconstruction, and discloses a cross-modal image reconstruction method and system based on wide field to total internal reflection fluorescence, and a medium. The method comprises the following steps: acquiring an EPI image and a corresponding TIRF image which are registered in the same field of view, and constructing a training data set; a convolutional neural network model with double-branch output is constructed, the wide-field fluorescence image is used as input, features are extracted through a convolutional layer, and then a foreground image and a background image with defocused backgrounds removed are reconstructed and output; carrying out convolution on the foreground image and a predefined point spread function to generate a virtual TIRF image; training the convolutional neural network model to obtain a wide-field fluorescence image reconstruction model; and performing EPI image reconstruction by using the wide-field fluorescence image reconstruction model. According to the method, the optical slice effect and the axial super-resolution capability which are close to those of a TIRF microscope can be obtained only by depending on a single EPI image for the first time, the problems of axial blurring and background of a wide-field image are solved, and the signal-to-noise ratio and the contrast ratio of the image are remarkably improved.

The invention discloses a microscopic fluorescence image deblurring method based on iterative optimization and Gaussian filtering, and relates to the technical field of image processing, and the method comprises the steps: collecting a to-be-processed microscopic fluorescence image, obtaining the image information, and generating a Gaussian filtering convolution kernel through a two-dimensional Gaussian function; performing convolution operation on the to-be-processed microscopic fluorescence image by using a Gaussian filtering kernel to generate an initial smooth image, the initial smooth image being preliminary estimation of image defocus information; the error weight is dynamically adjusted through an iterative optimization algorithm, the initial smooth image is updated until the number of iterations or convergence conditions are met, an optimized smooth image is obtained, and the optimized smooth image is accurate estimation of image defocus information; based on the optimized smooth image, performing deblurring processing through difference operation to obtain a deblurred image; and carrying out normalization processing on the deblurred image, and quantizing the deblurred image into a uint16 format for output. According to the invention, the dependence on high-precision hardware and complex optical elements is overcome, and a high-resolution optical slice imaging effect is realized.

The application discloses a kind of high-efficiency optical sectioning microscopic fluorescence imaging system and method.It includes: multi-wavelength beam combining laser module and single-mode optical fiber, collimating lens, first mirror, light splitting module, first optical path, second optical path, speckle generation module, fast switching module, second mirror, beam expander, third mirror, focusing lens, fluorescence module, tube lens, camera, objective lens, motorized displacement stage, condenser, illumination source and the like composition.For the current HiLo microscopic imaging speckle homogenization complex, uniform illumination and speckle illumination high-speed switching difficult, low light energy utilization efficiency and other technical problems;The application obtains the 2 images required for imaging by fast switching module and camera linkage efficiently;Compact structure design, effective utilization of system incident excitation light.The optical sectioning microscopic fluorescence imaging system of the application can realize the optical tomography of target sample efficiently and conveniently, and well expands the practical application range of HiLo in high-speed imaging.

The invention relates to the technical field of information optics, in particular to a near-infrared two-zone line scanning optical tomography microscopic imaging system and method based on a virtual digital slit, and the system comprises a near-infrared two-zone line scanning optical tomography microscopic imaging module. And the virtual digital slit image reconstruction module is used for reconstructing an image acquired by the near-infrared two-zone line scanning optical tomography microscopic imaging module. A digital micromirror device and a digital line array are introduced, exciting light is modulated by means of the high-speed signal modulation capacity of a DMD, the digital line array is used for replacing a physical slit in a traditional line scanning system, generation and rapid scanning of a parallel line array exciting light field are achieved, and the flexibility and imaging speed of the system are greatly improved; a virtual digital slit image reconstruction module is introduced to filter background noise, so that optical slicing is realized; a near-infrared two-area window is introduced into a line scanning optical tomography microscopic imaging system, so that the imaging depth, the resolution ratio and the signal-to-noise ratio of the system are further improved.

This ophthalmic device according to an exemplary embodiment comprises an illumination system and a plurality of imaging systems. The illumination system is configured to project illumination light onto an anterior segment of an eye under examination. Each imaging system is configured to satisfy the Scheimpflug condition. The plurality of imaging systems is configured to generate a plurality of items of data by capturing images in a state in which the respective imaging systems are focused on a plurality of different regions in an optical section formed by the illumination light.

An imaging system for imaging a biological sample or another sample containing fluorescent molecules may include an optical system with a light source emitting light, wherein the light is directed by the optical system to the sample via at least one plane being conjugate to the image plane. The optical source may have an extended radiation pattern, in other words, the radiation beam may have an extent in the x- and y-planes, rather than a point source. The extent of the illumination region in x and y may be based on and matched to the detector area onto which the radiation may be imaged, preferably a TDI detector. This novel system may include genomics, proteomics and transcriptomics work flows.