Fluorescent micro-spectrum imaging system with optical sectioning strength

Abstract

The invention discloses a fluorescent micro-spectrum imaging system with optical sectioning strength. The fluorescent micro-spectrum imaging system comprises a laser emitter, a beam-expanding light path component, a micro-reflector, a dichroscope, a fluorescent micro-spectrum imaging assembly and a spectrograph, wherein the laser emitter is used for emitting a laser beam to the beam-expanding light path component, and the beam-expanding light path component is used for carrying out speckle suppression to obtain an illuminating beam; the illuminating beam generates a structure light field through the micro-reflector; the structure light field is reflected to the fluorescent micro-spectrum imaging assembly through the dichroscope; the fluorescent micro-spectrum imaging assembly comprises an objective lens, an objective lens drive device and an objective table; the objective lens is used for receiving the structure light field and focusing on a sample on the objective table, and exciting a fluorescent beam; the objective lens drive device drives the objective lens to move along the optical axis, so that the objective lens carries out the optical axis scanning; and the spectrograph analyzes and separates the fluorescent beam to obtain spectrum and image information. By virtue of the fluorescent micro-spectrum imaging system with the optical sectioning strength, a three-dimensional image and corresponding one-dimensional spectrum information of the sample structure can be obtained.

Description

technical field [0001] The invention relates to an optical system, in particular to a fluorescence microspectral imaging system with optical tomography capability. Background technique [0002] Since its invention, the optical microscope has been one of the important tools for human beings to understand the microscopic world, and it plays a very important role in clinical medicine, biology, materials science and other disciplines. However, traditional optical microscopy can only obtain morphological information of objects such as cells, and cannot further analyze the structure and composition of objects. [0003] Spectral imaging technology is a new multi-dimensional information acquisition technology that combines imaging technology with spectral technology. The difference in the structure and composition of objects will have significant differences in the absorption spectrum. Imaging spectroscopy has become an extensive and effective means of detection in macroscopic spa...

AI Technical Summary

Problems solved by technology

[0005] At present, most fluorescence microspectral imaging systems based on traditional fluorescence microscopes are wide-field imaging methods with surface illumination. Although a complete image can be obtained in a short integration time, due to the interference of the out-of-focus part of the object , the image does not have the capability of three-dimensional tomography, and can only obtain two-dimensional image information and one-dimensional spectral information

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