Non-contact type optical sectioning imaging method

Abstract

The invention discloses a non-contact type optical sectioning imaging method, and solves the problem of the non-contact type optical sectioning imaging which can not be realized in the prior art under high-aperture lenses. The method comprises the following realization steps: firstly, using a multimode optical molecule imaging system to collect multi-angle optical images, organism surface three-dimensional shapes and anatomical structure information, then, reconstructing organism surface three-dimensional energy, including: (1) preprocessing optical images and conversing from gray value to energy value, (2) registering the optical images and organism surface three-dimensional shape information, (3) confirming virtual detecting surface position and pixel size, and mapping energy of CCD cameras to the virtual detecting surface, (4) computing visible factors, (5) reconstructing surface three-dimensional energy distribution, finally, inverting the internal light source, and displaying three-dimensionally and solidly. The method has the advantages of realizing surface three-dimensional energy reconstruction under high-aperture lenses and non-contact type sectioning imaging under high-aperture lenses, and can be used in the field of non-contact type optical molecule imaging.

Description

technical field [0001] The invention belongs to the technical field of optical molecular imaging, and relates to a non-contact optical tomographic imaging technology, in particular to an imaging method for indirect information from a multi-angle two-dimensional optical image measured by a non-contact CCD camera to an internal light source of a living body. Background technique [0002] In recent years, optical molecular imaging technology has been widely used in small animal research due to its high sensitivity, low price and easy operation. In order to obtain an intuitive three-dimensional visualization imaging effect, the newly developed optical tomography imaging technology of optical molecular imaging technology has become a new application research hotspot. In vivo tissue lesion information. In optical tomography, excited fluorescence tomography and bioluminescence tomography are two main imaging modalities. [0003] Optical tomography technology reconstructs the ligh...

AI Technical Summary

Problems solved by technology

However, this method is only applicable to the case of small-aperture lenses, and is not suitable for slightly larger-aperture lenses or large-aperture lenses, so it is impossible to realize the problem of non-contact optical tomography in the case of large-aperture lenses

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