7 results about "Bioluminescence" patented technology

This invention relates to a recombinant construct comprising: one or more TMV Ω enhancers; and a group of fluorescent factors configured to enable an organism or its tissues or cells to emit bioluminescence; the recombinant construct is configured to increase the biosynthetic content of caffeic acid in an organism or its tissues or cells, or to increase the bioluminescence intensity of an organism or its tissues or cells. This application, by introducing TMV Ω enhancers, achieves precise regulation of these two key genes, significantly improving bioluminescence intensity and solving the problems of poor targeting and limited synergistic effects of enhancer applications in existing technologies.

This invention belongs to the field of immunoassay detection and discloses a ZrMPNs-NlucmAb bioluminescent signal probe and its application. The probe consists of a ZrMPNs signal carrier and its electrostatically adsorbed bioluminescent enzyme-labeled monoclonal antibody NlucmAb. This invention utilizes the ZrMPNs-NlucmAb probe to construct a bioluminescent test strip (BLFA), which requires no external light source and achieves a high luminescent signal in a furazine (FZ)-Nluc system, thus enabling sensitive detection of OTA. The limit of detection (LOD) of BLFA is 0.02 ng / mL, which is 9 times more sensitive than traditional gold nanoparticle-based LFAs. The cross-reactivity rates of this BLFA to OTB and OTC are 122.2% and 111.1%, respectively, and it does not recognize other common fungal toxins, exhibiting good specificity. Its application in the detection of OTA in cereals shows great promise.

A nucleic acid comprising a continuous nucleotide sequence, containing: (i) a gene encoding LuxA, (ii) a gene encoding LuxB, (iii) a gene encoding LuxC, (iv) a gene encoding LuxD, (v) a gene encoding LuxE, wherein each of the genes is under the control of a promoter heterologous to the respective gene, and wherein all of the genes together with the promoter are contained in a single nucleotide sequence in a row. The vectors and host cells comprise the nucleic acid. Methods of producing a host cell and methods and uses for detecting an environmental effect and a kit therefor.

The present invention concerns bioluminescent plants and methods for producing them. Among said methods, the invention relates, in particular, to a method of introducing luciferase genes and luciferin biosynthesis genes into the chloroplasts of a plant. In addition, the invention concerns a method of producing light comprising adding chemical compounds to a culture medium of the plant according to the invention.

A method for correcting the amount of light emitted from living tissue that produces a light emission phenomenon is provided. [Solution] A method for correcting the amount of light emitted by a biological tissue causing a luminescence phenomenon inside a living organism by applying an AC magnetic field to a magnetic fluid injected into the biological tissue causing a luminescence phenomenon to heat the biological tissue causing the luminescence phenomenon, and correcting the measured amount of light emitted by the biological tissue causing the luminescence phenomenon before and after heating includes the steps of determining, outside the living organism, the change in the amount of light emitted relative to the amount of magnetic fluid; heating the biological tissue causing the luminescence phenomenon and calculating the amount of magnetic fluid contained in the biological tissue causing the luminescence phenomenon based on a time constant related to the temperature change of the biological tissue causing the luminescence phenomenon; measuring the amount of light emitted by the biological tissue causing the luminescence phenomenon into which magnetic fluid has been injected; and calculating a correction value for the measured amount of light emitted based on the calculated amount of magnetic fluid.