40 results about "Holothuria scabra" patented technology

The invention discloses a method for ecological breeding of holothuria scabra in a halophila oralis transplanting sea grass bed. The method comprises the following technical steps of pond condtion setting, processing before transplanting, halophila oralis investigation, halophila oralis collection, halophila oralis transplanting, holothuria scabra breeding and pond water supplementing. The grass planting time and holothuria scabra specification are reasonably selected, the holothuria scabra is bred in the halophila oralis transplanting sea grass bed, and the ecological functions of the halophila oralis transplanting sea grass bed are successively utilized and embodied. No water change nor oxygen increase is needed in the breeding process, no drug is used, the cost is low, a breeding effect is good, and the method can serve as an efficient and ecological holothuria scabra breeding method. References are provided for sea grass resource recovery and holothuria scabra breeding in diversified modes. The weight of each holothuria scabra bred by adopting the method is up to 42.7-43.5 g, and the breeding time is 60 days.

The invention discloses a DNA (Deoxyribonucleic Acid) barcode molecular identification method of two types of sea cucumbers (holothuria fuscopunctata and holothuria scabra) and a CO I sequence of the sea cucumbers. The method is used for carrying out PCR (Polymerase Chain Reaction) amplification to obtain a CO I gene of the sea cucumbers; a PCR product is identified through agarose gel and then is sequenced; a sequencing result is compared with a public sequence; if the homology between the sequencing result and a gene sequence SEQ ID NO.1 is 99 percent or above, the method can judge that a tissue to be detected is a source of the holothuria fuscopunctata; if the homology between the sequencing result and a gene sequence SEQ ID NO.2 is 99 percent or above, the method can judge that the tissue to be detected is the holothuria scabra. According to the DNA barcode molecular identification method, a reliable DNA technology is adopted, and holothuria fuscopunctata and holothuria scabra varieties are rapidly and accurately distinguished; the accuracy and reliability are enhanced, and the safety of taking the sea cucumbers as a medicinal material is ensured.

The invention discloses a method for identifying female ginseng individuals in the ovary development stage of rough sea cucumbers. The body cavity fluid of the rough sea cucumber was extracted, and then the yolk protein was separated by centrifugation, and then subjected to SDS-PAGE electrophoresis and staining, and the electrophoresis results were used to distinguish the female ginseng in the ovarian development stage, the male ginseng and the rough sea cucumber with immature gonads. The individual samples of female ginseng at the stage of ovarian development showed a characteristic band of 200‑250kD, while the individual samples of gonadal anaplastic individual and male ginseng did not appear this characteristic band. The invention can identify female ginseng individuals in the ovarian development stage by extracting body cavity fluid samples without damaging the rough sea cucumber, and has application value for the selection of parent ginseng during artificial seedling cultivation.

The invention discloses a method for maturing wild Holothuria scabra by manual intensive culture in an indoor cement pond. The method is carried out by the following steps of: collecting grown-up sea cucumbers of wild Holothuria scabra->fostering and screening the grown-up sea cucumbers->washing and disinfecting a breeding pond->modifying the breeding pond->feeding water into the breeding pond->disinfecting the grown-up sea cucumbers->putting the grown-up sea cucumbers into the pond->carrying out daily management measures. In the invention, by utilizing comprehensive technical measures combining the modification of environment conditions and intensification of inner nutrition, conditions including water quality, bottom materials, light illumination and the like which are close to wild habitats of the wild Holothuria scabra are created in the indoor cement pond; meanwhile, artificial compound feed with comprehensively-balanced nutrition is fed, scientific daily management is carried out to enable the wild Holothuria scabra from the natural sea to be better adapted to the indoor cultivation environment, and the survival rate reaches more than 95%; meanwhile, the wild Holothuria scabra can rapidly grow and the weight is increased from 100-200 g to 300 g in a short period (30-40 days); the growth of sexual glands of the wild Holothuria scabra are mature so that the wild Holothuria scabra can be used as seed sea cucumbers and the ratio of the group mature seed sea cucumber reaches 20%.