40 results about "Holothuria scabra" patented technology

The present invention discloses a process of extraction, purification and characterization of a non-polar fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye, said dye is a natural, cell permeant, nontoxic and environmentally friendly non-polar fluorescent dye.

The invention provides a holothuria scabra artificial seeding breeding method. The method comprises the steps such as parent holothurian cultivating, induced spawning, spawn washing and hatching, larva cultivating, seedling collecting and childish holothurian cultivating, wherein spirulina powder, marine yeast, aquatic yeast and bacillus serve as bait, not only are bait sources convenient, but also the investment of single cell culture facilities is saved, meanwhile, manpower needed by single cell algae bait culture is saved, the holothurian seedling cost can be greatly saved, and the popularization of the holothuria scabra seedling breeding technology is facilitated. The substitutive bait is adopted to replace living single cell algae, invested funds of seeding facilities can be saved by about 25 percents, the human input of seeding can be saved by about 30 percents, and the ratio of the seeding input to the seeding output 1:3. Induced spawning is performed on the two days before or after the first day and the fifteenth day of a lunar month, the good effect of two times of spawn sampling in one month is achieved, the induced spawning success rate is 83.3 percents, and the technical problem that the induced spawning difficulty of holothuria scabra parent holothurian is high is effectively solved.

The invention relates to the technical field of a feed, and particularly relates to a compound feed for enhancing an immune function of a laying hen. The feed is prepared from the following raw materials by weight percent: 12-15 percent of sorghum bran, 65-80 percent of sweet corn, 15-18 percent of second heads, 15-18 percent of rice bran cake, 10-12 percent of barley malt roots, 15-20 percent of bean pulp, 10-12 percent of peanut bran, 2-3 percent of mountain flour, 2-3 percent of margarya melanoide dry powder, 2-3 percent of caterpillar powder, 1-2 percent of duck blood powder, 1-2 percent of whey powder, 0.2-0.5 percent of prepared rehmannia root, 0.2-0.3 percent of common yam rhizome, 0.5-1 percent of holothuria scabra jaeger, 0.5-1 percent of oldenlandia diffusa, 0.5-1 percent of platycodon grandiflorum, 2-3 percent of melon seeds, 1-2 percent of bitter melon seeds, 4-5 percent of capsella bursa-pastoris, 2-3 percent of potato peel, a proper amount of salt and 0.2-0.5 percent of phagostimulant. The feed for the laying hen provided by the invention is abundant in raw materials, and sufficient in nutrition; the especially added traditional Chinese medicine ingredients can be used for effectively enhancing the immunity of the laying hen; development and repair of immune cells are facilitated; the morbidity of common diseases of the laying hen is effectively reduced.

The invention discloses a method for maturing wild Holothuria scabra by manual intensive culture in an indoor cement pond. The method is carried out by the following steps of: collecting grown-up sea cucumbers of wild Holothuria scabra->fostering and screening the grown-up sea cucumbers->washing and disinfecting a breeding pond->modifying the breeding pond->feeding water into the breeding pond->disinfecting the grown-up sea cucumbers->putting the grown-up sea cucumbers into the pond->carrying out daily management measures. In the invention, by utilizing comprehensive technical measures combining the modification of environment conditions and intensification of inner nutrition, conditions including water quality, bottom materials, light illumination and the like which are close to wild habitats of the wild Holothuria scabra are created in the indoor cement pond; meanwhile, artificial compound feed with comprehensively-balanced nutrition is fed, scientific daily management is carried out to enable the wild Holothuria scabra from the natural sea to be better adapted to the indoor cultivation environment, and the survival rate reaches more than 95%; meanwhile, the wild Holothuria scabra can rapidly grow and the weight is increased from 100-200 g to 300 g in a short period (30-40 days); the growth of sexual glands of the wild Holothuria scabra are mature so that the wild Holothuria scabra can be used as seed sea cucumbers and the ratio of the group mature seed sea cucumber reaches 20%.

The invention relates to an intermediate culture method for Holothuria scabra Jaeger, and belongs to the technical field of aquatic product culture. The method comprises the two aspects of indoor cement pond intermediate culture and outdoor net cage pond intermediate culture. According to the aspect of indoor cement pond intermediate culture, the steps such as water quality control, intermediate culture density control, bait casting, water changing and pouring into a pond, inflation, disease control, daily management and intermediate culture time control are included. According to the aspect of outdoor net cage pond intermediate culture, the steps such as preparation before seedling release, seedling feeding, bait feeding, water changing and seedling thinning are included. The method facilitates management and is low in labor intensity, the quality and survival rate of the whole young Holothuria scabra Jaeger can be improved, economic benefits can be improved, and the method is suitable for application and popularization in the aspect of aquaculture.

The invention relates to medicine technical field, in particular to two saponin antimycotics compounds as scabraside A and scabraside B, which molecular formulas are represented as C54H85O26SNa and C54H85O27SNa, separated from sea cucumber. The chemical structure and spatial configuration of the compounds are assured by kinds of modern spectrographic analysis, particularly by kinds of advanced two-dimension nuclear magnetic resonance spectroscopy. The compounds can effectively restrain various bacterial strains as candida albicans and aspergillus fumigatus by external antimycotics test. The inventive compounds can be used to prepare antimycotics drug. The invention provides lead compound for preparing new antimycotics drug, with significant value for developing and utilizing sea medical biological resource.

The present invention provides a novel compound multiple fluorescent natural dye from a marine organism Holothuria Scabra having a chemical structure of of Si—O—R type wherein silica matrix is an integral part of the compound, taking part in the metabolism of the animal, wherein R being an organic moiety and also provides a composition containing the compound having various applications; also a process of extraction, purification and characterization of the novel multiple fluorescent natural dye compound from a marine organism (Holothuria scabra).

The invention discloses a wintering method for parent holothuria scabra. The wintering method for the parent holothuria scabra comprises the steps of selecting and treating the parent holothuria scabra for wintering, building a wintering system for the parent holothuria scabra, putting the parent holothuria scabra into the parent holothuria scabra wintering system, managing the wintering environment, managing feeding, regulating and controlling water quality, preventing and treating disease, and the like. The wintering system for the parent holothuria scabra comprises a wintering pool, a heat preservation shed and a water feeding and discharging system. As the wintering method for the parent holothuria scabra meets various requirements of wintering of the parent holothuria scabra, the parent holothuria scabra can winter safely. According to the wintering method, the wintering survival rate of the parent holothuria scabra can be substantially increased, the survival rate can reach more than 90 percent, the growth rate of body mass can reach more than 23 percent, and gonadal development quality is good. According to the wintering method and system, the current wintering problem for the parent holothuria scabra can be effectively solved, the robust parent holothuria scabra can be provided for artificial breeding of the holothuria scabra, and therefore farming benefits can be effectively achieved.

The invention discloses a DNA (Deoxyribonucleic Acid) barcode molecular identification method of two types of sea cucumbers (holothuria fuscopunctata and holothuria scabra) and a CO I sequence of the sea cucumbers. The method is used for carrying out PCR (Polymerase Chain Reaction) amplification to obtain a CO I gene of the sea cucumbers; a PCR product is identified through agarose gel and then is sequenced; a sequencing result is compared with a public sequence; if the homology between the sequencing result and a gene sequence SEQ ID NO.1 is 99 percent or above, the method can judge that a tissue to be detected is a source of the holothuria fuscopunctata; if the homology between the sequencing result and a gene sequence SEQ ID NO.2 is 99 percent or above, the method can judge that the tissue to be detected is the holothuria scabra. According to the DNA barcode molecular identification method, a reliable DNA technology is adopted, and holothuria fuscopunctata and holothuria scabra varieties are rapidly and accurately distinguished; the accuracy and reliability are enhanced, and the safety of taking the sea cucumbers as a medicinal material is ensured.

The invention discloses a method for mixed culture of Holothuria scabra and Litopenaeus vannamei in an indoor pond. Technical measures including concrete pond conditions, culture pretreatment, larva stocking, bait casting, water quality control and disease control, Litopenaeus vannamei aftertreatment, Holothuria scabra aftertreatment and sediment treatment after culture are adopted according to biological characteristics of the Holothuria scabra and the Litopenaeus vannamei, and reasonable arrangement of larva specifications and larva casting time is combined with culture technologies of the Holothuria scabra and the Litopenaeus vannamei, so that ecologically complementary and mutually beneficial mixed culture of the Holothuria scabra and the Litopenaeus vannamei is achieved. The Holothuria scabra ingests organic matters and scraps decomposed by residual baits, metabolites of the Litopenaeus vannamei, microorganisms and bacteria, so that survival rates and growth speeds of the Holothuria scabra and the Litopenaeus vannamei are increased, and the Litopenaeus vannamei has a remarkable ecological effect on improvement in the water quality and sediments. The method for mixed culture of the Holothuria scabra and the Litopenaeus vannamei in the indoor pond is simple, convenient, efficient, highly operable and capable of improving the culture water quality and the sediments so as to achieve indoor efficient ecological culture of the Holothuria scabra and the Litopenaeus vannamei.

The present invention discloses the process of extraction, purification and characterization of a fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye.

The invention discloses a method for ecological breeding of holothuria scabra in a halophila oralis transplanting sea grass bed. The method comprises the following technical steps of pond condtion setting, processing before transplanting, halophila oralis investigation, halophila oralis collection, halophila oralis transplanting, holothuria scabra breeding and pond water supplementing. The grass planting time and holothuria scabra specification are reasonably selected, the holothuria scabra is bred in the halophila oralis transplanting sea grass bed, and the ecological functions of the halophila oralis transplanting sea grass bed are successively utilized and embodied. No water change nor oxygen increase is needed in the breeding process, no drug is used, the cost is low, a breeding effect is good, and the method can serve as an efficient and ecological holothuria scabra breeding method. References are provided for sea grass resource recovery and holothuria scabra breeding in diversified modes. The weight of each holothuria scabra bred by adopting the method is up to 42.7-43.5 g, and the breeding time is 60 days.

The invention relates to a Chinese herbal medicine composition for treating leukemia, belonging to the technical field of Chinese medicines. The composition consists of two prescriptions which complement with each other and consolidate effects mutually. Major components of the special effect Chinese herbal medicine composition include garter snake, panax pseudo-ginseng, parslane herb, dried alum, crude arisaema consanguineum schott, holothuria scabra jaeger, depositum urinae hominis, puffball, interior eardamom, Chinese actinidia root, blackberry lily, white mustard seed, mole cricket and eupolyphaga sinensis walker. The Chinese medicinal composition has good effects on clinical common anemia, fever, infection, hemorrhage and liver, speech and lymphadenectasis; and according to a second prescription, neoplastic hyperplasia of leukemia cells in marrow or other hemopoietic tissues can be eliminated, and the physique of a patient can be enhanced simultaneously.

The invention relates to a simple and convenient feeding device applicable to indoor culture of holothuria scabra. The feeding device comprises a plastic container for containing feed, and a support for fixing the plastic container. A hopper is arranged at the bottom of the plastic container and connected with a feed conveying guide pipe. An adjusting switch for adjusting the flow speed of feed is arranged on the feed conveying guide pipe. The feed conveying guide pipe is connected with a feed feeding guide pipe. The feed feeding guide pipe is flatly laid at the bottom of a culture pond. Feed feeding holes are formed in the feed feeding guide pipe every 20-50 cm. The hole diameters of the feed feeding holes are gradually increased from 1-6 cm from the end connected with the feed conveying guide pipe to the far end, and a lead weight for fixing the guide pipe is arranged on the feed feeding guide pipe. The feeding device is simple in structure, low in manufacturing cost and easy to prepare, raw materials are easy to obtain, the amount of feed is saved after the feeding device is in use, substrate and water pollutions are reduced, and holothuria scabra disease is lowered.

The invention relates to a method for pond culture of Holothuria scabra Jaeger, and belongs to the technical field of aquaculture. The method specifically comprises the four steps of environment and condition development, seedling selection, pond cleaning and pest removing and development management. According to the growth and culture characteristics of the Holothuria scabra Jaeger, seedlings which are in intermediate culture for about 30 days are used as offspring seedlings for developing pond culture production. According to the method for the pond culture of the Holothuria scabra Jaeger, the conditions of the nature are fully utilized for adjusting the culture environment, the bionic effect is good, no bait needs to be cast, water can be changed along with tides, the amount of bait is saved, and the water purification cost and the labor cost are reduced.

The invention relates to application of Vc-2-triphosphate in preparation of medicine for preventing and treating the holothuria scabra skin ulcer syndrome. Vc-2-triphosphate is an immunopotentiator, can participate in a redox reaction in holothuria scabra bodies and synthesis of active protease, can enhance the anti-stress capacity of the organism immunity and disease resistance of holothuria scabra, and further has ascorbic activity and good stability. In addition, under participation of Vc-2-triphosphate, povidone iodine disinfectant is promoted to continuously release free iodine to damage metabolism of thalli, bacteria and other microorganisms are inactivated, and a good killing effect is achieved on bacteria, viruses and fungi. The preventing and treating effect of Vc-2-triphosphate applied to the holothuria scabra skin ulcer syndrome is obvious through the chemical features of the two substances.

The invention belongs to the technical field of aquaculture, and particularly relates to a method for testing the fecundity of holothuria scabra. The method comprises the implementation steps of holothuria scabra treating, weighing and dissecting, sampling, wall breaking and counting; fecundity calculating, wherein the absolute fecundity and relative fecundity of holothuria scabra are calculated according to the following formulas respectively: absolute fecundity=1/2*We*(N1/W1+N2/W2), and relative fecundity=1/2*We/Wt*(N1/W1+N2/W2). The simple method is utilized, a small branch tube wrapping eggs is broken through a dissecting needle, most of the eggs are released out of a tube cavity, and therefore the eggs can be counted under a microscope, and the fecundity of holothuria scabra is tested. The method is easy to operate, simple and feasible, serves development of artificial reproduction and production of holothuria scabra and can provide reference for testing the fecundity of sea cucumbers of other types.