4 results about "Benthic diatom" patented technology

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

A method for improving the mating rate of Penaeus vannamei, comprising the following steps: step one, cultivating diatoms in a parent culture pond: scraping the naturally propagated benthic diatoms from the inner wall of the water inlet pipeline in the breeding field or near the middle tide line of the beach to obtain the benthic diatom liquid; culturing the benthic diatoms in still water: continuously adding filtered seawater to culture the benthic diatoms by flowing water; and culturing in a pond: moving female and male parent shrimps into the parent culture pond in which the benthic diatoms are cultivated until the female shrimps mature; step two, moving the female shrimps into the male shrimp culture pond for mating: selecting the female shrimps with mature gonads and moving them into the male shrimp culture pond for mating; step three, moving the female shrimps that have successfully mated into an egg laying pond for laying eggs, and taking out the female shrimps after laying eggs and returning them to the original culture pond. The method stabilizes the water quality by managing the benthic diatoms in the culture pond, provides a stable mating environment, and improves the mating rate; the operation is simple, the parent shrimps are less damaged, the cost is lower, the success rate is high, and the mating rate is as high as 90%.

The application discloses a method for improving survival of Trochus conus seedlings. A plastic film or corrugated plate is placed in a water pool, an algal nutrient solution is added, water flow is suspended, and aeration culture is carried out for 15-30 days. A black sunshade net is installed above the water pool, so that the light intensity is lower than 5000 Lux. The temperature of the culture water body is 20-30 DEG C, and the salinity is 30-32 ppt. After the benthic diatoms grow fully, the water in the water pool is completely discharged, the sunshade net is lifted, the benthic diatoms are exposed to sunlight, and the exposure is continued until part of the benthic diatoms die. Then, water is added to the water pool for aeration, the sunshade net is laid again, and the Trochus conus fertilized eggs are moved into the diatom pool. Water flow is suspended, and aeration culture is carried out. The sunlight exposure method is used to kill about 30%-70% of the benthic diatoms, and part of the fresh benthic diatoms are reserved, so that the attachment metamorphosis and survival of the Trochus conus larvae are obviously improved, and technical support is provided for large-scale production of the Trochus conus.

This invention discloses a foamed concrete material for artificial reefs and its preparation method, belonging to the technical field of marine engineering building materials. It uses silicate cement, slag powder, fly ash, and metakaolin as the cementing system, compounded with a grafted dynamic alkalinity regulator, an amphiphilic polymerizable precursor, and ecologically cascaded inducing microcapsules. The precursor forms an organic-inorganic interpenetrating network in situ at the gas-liquid interface to reinforce the pore walls; the alkalinity regulator stabilizes the pore fluid pH for a long time through a two-site buffer; and the microcapsules achieve a gradual release of nutrients through a multi-level gated structure. This material exhibits a compressive strength of 5.4-5.8 MPa at high porosity, low water absorption, a stable pore fluid alkalinity reduction to the weakly alkaline range, slow nutrient release without sudden release, and high benthic diatom attachment. It combines good mechanical durability with ecological compatibility and can be used in marine ranching artificial reef engineering.