7 results about "Benthic diatom" patented technology

The invention discloses a preparation method and application of an anti-fouling coating system capable of preventing biological adhesion, the anti-fouling coating system capable of preventing biological adhesion comprises a primer and a topcoat, and the primer comprises a first component and a second component. According to the preparation method and application of the anti-fouling coating system capable of preventing biological adhesion, the anti-traditional thinking is used for developing an anti-fouling coating, the surface coating is used for developing a nano composite particle coating, a high-surface-energy nano material is firstly used as a basis, a dark catalytic electrolysis technology is matched for preparation, the characteristic of high surface energy of the nano material is adopted, and the anti-fouling coating system capable of preventing biological adhesion is prepared. Energy is absorbed, stored, converted and released through the composite nano-material, the nano-material breaks walls of marine microorganisms and a generated electric field effectively prevents generation of a biological membrane, then growth of marine bacteria, shells and benthic diatom is effectively inhibited, meanwhile, two-dimensional nano-graphene is adopted to replace traditional graphene oxide for modification, the overall engineering is simple, and the cost is low. More atoms are distributed on the surface, and the coating surface has higher energy.

The invention relates to the technical field of microbial culture, in particular to a heterotrophic culture method of benthic diatom, which comprises the following steps: S1, adding benthic diatom into an improved SE culture medium, and culturing for 5-7 days until OD540 is 0.8-1.2 to obtain a benthic diatom seed solution; according to the method disclosed by the invention, a nutrition system is constructed by taking waste glycerol as a carbon source and sodium alginate coated urea as a slow-release nitrogen source, and the benthic diatom, nitrogen-fixing bacteria and phosphate solubilizing bacteria are co-cultured on a porous ceramic carrier to be cultured under a dark condition, so that compared with the prior art, the method disclosed by the invention thoroughly gets rid of the dependence on illumination, and the yield is increased. The rapid, high-yield and stable heterotrophic culture of the benthic diatom is realized, meanwhile, the raw material and energy consumption cost is greatly reduced, and the resource recycling rate is improved; the obtained diatom biomass can be used for aquatic bait, biological energy, environmental restoration and development of high-added-value products, and has a wide industrial application prospect.

A method for improving the mating rate of Penaeus vannamei, comprising the following steps: step one, cultivating diatoms in a parent culture pond: scraping the naturally propagated benthic diatoms from the inner wall of the water inlet pipeline in the breeding field or near the middle tide line of the beach to obtain the benthic diatom liquid; culturing the benthic diatoms in still water: continuously adding filtered seawater to culture the benthic diatoms by flowing water; and culturing in a pond: moving female and male parent shrimps into the parent culture pond in which the benthic diatoms are cultivated until the female shrimps mature; step two, moving the female shrimps into the male shrimp culture pond for mating: selecting the female shrimps with mature gonads and moving them into the male shrimp culture pond for mating; step three, moving the female shrimps that have successfully mated into an egg laying pond for laying eggs, and taking out the female shrimps after laying eggs and returning them to the original culture pond. The method stabilizes the water quality by managing the benthic diatoms in the culture pond, provides a stable mating environment, and improves the mating rate; the operation is simple, the parent shrimps are less damaged, the cost is lower, the success rate is high, and the mating rate is as high as 90%.

The present invention discloses a method for improving the peeling specifications of sea urchin seedlings, comprising the following steps: (1) inoculating Oscillatoria onto the front of a bait plate, and inoculating Oscillatoria onto the back of the bait plate the next day, and allowing the Oscillatoria to stand and cultivate; (2) inoculating benthic diatoms onto the front of the bait plate that has been inoculated with Oscillatoria, and inoculating benthic diatoms onto the back of the bait plate that has been inoculated with Oscillatoria the next day. The present invention first inoculates Oscillatoria on the bait plate, and then inoculates benthic diatoms, so that Oscillatoria can grow together with benthic diatoms on the bait plate. After the benthic diatoms are eaten to a certain extent by the sea urchin seedlings, suitable water temperature, light and nutritional conditions are provided to allow the Oscillatoria to grow rapidly, thereby meeting the bait needs of the sea urchin seedlings when they lack benthic diatoms, achieving a larger peeling specification, and even reaching a shell diameter of 4-5 mm, thereby ensuring the survival rate of the sea urchin seedlings after peeling.

The application discloses a method for improving survival of Trochus conus seedlings. A plastic film or corrugated plate is placed in a water pool, an algal nutrient solution is added, water flow is suspended, and aeration culture is carried out for 15-30 days. A black sunshade net is installed above the water pool, so that the light intensity is lower than 5000 Lux. The temperature of the culture water body is 20-30 DEG C, and the salinity is 30-32 ppt. After the benthic diatoms grow fully, the water in the water pool is completely discharged, the sunshade net is lifted, the benthic diatoms are exposed to sunlight, and the exposure is continued until part of the benthic diatoms die. Then, water is added to the water pool for aeration, the sunshade net is laid again, and the Trochus conus fertilized eggs are moved into the diatom pool. Water flow is suspended, and aeration culture is carried out. The sunlight exposure method is used to kill about 30%-70% of the benthic diatoms, and part of the fresh benthic diatoms are reserved, so that the attachment metamorphosis and survival of the Trochus conus larvae are obviously improved, and technical support is provided for large-scale production of the Trochus conus.

The application discloses a method for reducing large benthic diatom adhesion on Undaria pinnatifida gametophyte seedling curtain, which comprises the following steps: (1) placing several transparent thin plates on the upper surface of the seedling curtain after Undaria pinnatifida gametophyte is poured into a pool, and the transparent thin plates are uniformly distributed on the seedling curtain; (2) replacing the transparent thin plates when water flows for 48 hours after the gametophyte is poured into the pool, and then replacing the transparent thin plates once a day; (3) removing the transparent thin plates when the Undaria pinnatifida gametophyte seedling emergence rate reaches more than 60%, and then entering normal management until the seedling is delivered out of the warehouse. The transparent thin plates are placed on the upper surface of the seedling curtain after the Undaria pinnatifida gametophyte is poured into the pool, so that the large benthic diatom pollution is prevented. The method is simple in operation, and only needs to place the transparent thin plates on the seedling curtain; the cost is low, and the effect of preventing the large benthic diatom can be achieved by placing the transparent thin plates in the Undaria pinnatifida gametophyte seedling breeding pool.

This invention discloses a foamed concrete material for artificial reefs and its preparation method, belonging to the technical field of marine engineering building materials. It uses silicate cement, slag powder, fly ash, and metakaolin as the cementing system, compounded with a grafted dynamic alkalinity regulator, an amphiphilic polymerizable precursor, and ecologically cascaded inducing microcapsules. The precursor forms an organic-inorganic interpenetrating network in situ at the gas-liquid interface to reinforce the pore walls; the alkalinity regulator stabilizes the pore fluid pH for a long time through a two-site buffer; and the microcapsules achieve a gradual release of nutrients through a multi-level gated structure. This material exhibits a compressive strength of 5.4-5.8 MPa at high porosity, low water absorption, a stable pore fluid alkalinity reduction to the weakly alkaline range, slow nutrient release without sudden release, and high benthic diatom attachment. It combines good mechanical durability with ecological compatibility and can be used in marine ranching artificial reef engineering.