The invention belongs to the industry of aquaculture and particularly relates to a benthic diatom culture method used for culturing breeding feed. The method is characterized by comprising the following steps: inoculating navicula tenera obtained by separation and purification and nitzchia palea to a liquid F culture medium in certain cell density, putting a circulating pump in the liquid F culture medium, and performing intermittent circulation, namely performing circulation for 10 minutes at intervals of every 2 hours, wherein the formula of the improved F culture medium comprises 149.6mg ofNaNO3, 8.8mg of NaH2PO4, 3.9mg of FeC6H5O7.5H2O, 1mL of trace element solution mother liquor, 1mL of vitamin solution mother liquor, 1000mL of pure water, vitamin mother liquor, 100mg of vitamin B1,0.5mg of vitamin B12 and 500mL of pure water; culturing benthic diatom in a 5000mL conical flask; and normally boiling seawater by adopting the improved F culture medium, wherein the salinity is 31, the illumination intensity is 50mu mol E.m<-2>s<-1>, the illumination period is 16L:8D, and the temperature is 20 DEG C. The benthic diatom culture method is simple, convenient and fast in operation, low in cost and suitable for popularization and application.