63results about How to "Promote division and proliferation" patented technology

The present invention provides a liquid for preserving cornea for medium period and a preparing method thereof, and relates to a liquid for cornea material. The invention provides the liquid for preserving cornea for medium period and the preparing method thereof, wherein the liquid has the advantages of longer storing time, higher quality, no pollution and relatively lower cost compared with theprior liquid for preserving cornea for medium period. The liquid for preserving cornea for medium period contains culture solution, thickening agent, bovine serum, amino acid, antibiotic, acid-alkalimodifying agent and cell nutrition components. According to the formula of liquid for preserving cornea for medium period, the thickening agent, the bovine serum, the amino acid, the antibiotic and cell nutrition components are added into the culture solution for mixing to uniform. The pH value is adjusted to 7.2-7.4 with the acid-alkali modifying agent. The osmotic pressure is adjusted to 350-380mOsm/L with an osmotic pressure buffering agent. The liquid for preserving cornea for medium period is obtained after membrane filtering for sterilizing.

The invention discloses a larimichthys crocea liver cell line and an establishment method thereof. The larimichthys crocea liver cell line is preserved in the China center for type culture collection (CCTCC) On October 31, 2013 and has a preservation number of CCTCC NO: C2013157. The larimichthys crocea liver cell line can realize serial passage, can provide a large amount of larimichthys crocea liver cell lines, can be used for pathogenic characteristic research, vaccine development, functional gene research and breeding research, has excellent properties, comprises fibroblast-like cells and mainly comprises cells having parapodiums stretching out and irregular shapes. The larimichthys crocea liver cell line has the advantages of exuberant division, short passage time, easy digestion, good adherence rate and hunger resistance. The establishment method has good repeatability. The larimichthys crocea liver cell line has good stability.

The dynamic 3D input tissue reactor includes one culture case for inoculating cell onto biological rack with central channel; at least one canal stretched into the central channel of the biological rack, and one circular infusion device communicated with the canal. The culture case has one inlet port and one outlet port, and via the inlet port the canal is communicated with the circular infusion device. Via the closed circular infusion device, suspended cell liquid is fed directly to the central position of biological rack material inside the culture case, and after the cell attached to wall of the biological rack, micro cell culturing liquid is fed continuously with micro circulating pump to provide cell with enough nutrients for cell division and proliferation to form new tissue.

The invention relates to an application of a medicinal salt or a derivative of heparin and low molecular heparin for preparing an oral medicament composition, belonging to the field of medicines. The oral medicament composition enables a diabetic to recover after long-time treatment of diabetes, can prevent a patient who has reduced sugar tolerance from becoming a diabetic and can improve the vitality of islet cell beta. The medicinal application of the medicinal salt or the derivative of the heparin and the low molecular heparin has significant treatment effect and is quite suitable for clinical application.

The invention discloses a larimichthys crocea testis tissue cell line and application thereof. The larimichthys crocea testis tissue cell line is a larimichthys crocea testis tissue cell line LYCTs, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO:C202118. The larimichthys crocea testis tissue cell line disclosed by the invention is excellent in cell character, fuller in cell morphology and in a long fiber shape, the constructed cell line is good in stability, the blank of research on the larimichthys crocea testis tissue cell line is filled, and a new research tool is provided for research on a gonad development mechanism of larimichthys crocea.

The invention provides a Takifugu bimaculatus ovarian tissue cell line and application thereof, and belongs to the technical field of biology. According to the method, ovaries of the takifugu bimaculatus are subjected to isolated culture to obtain primary cells of the ovaries, and continuous subculture is performed to finally establish the stable ovarian cell line of the takifugu bimaculatus. The cell line is excellent in cell character, mainly comprises long fibrous cells, and is vigorous in division, short in passage time, easy to digest, good in adherence rate and resistant to hunger. The takifugu bimaculatus ovary cell line provides a new in-vitro model for research on a gonad development mechanism of takifugu bimaculatus.

The invention discloses a large yellow croaker ovarian tissue cell line and application thereof. The large yellow croaker ovarian tissue cell line is a large yellow croaker ovarian tissue cell line LYCO and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202117. The large yellow croaker ovarian tissue cell line is excellent in cell character, mainly composed of long fibrous cells, easy to digest, good in adherence rate and resistant to hunger, and the constructed cell line is good in stability. The blank of research on the large yellow croaker ovarian tissue cell line is filled, and a new research tool is provided for research on the gonad development mechanism of the large yellow croaker.

The invention belongs to the technical field of biology, and particularly discloses a mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium comprises a basal culture medium and additives, wherein the additives comprise non-essential amino acids, a pH regulator, antibiotics, organic substance components for promoting cell membrane synthesis, cell matrix components, cell growth factors, vitamins, trace elements and an antioxidant. The mesenchymal stem cell culture medium can provide nutrient substances required by cell growth for stem cell growth, maintain the cell growth environment and the cell activity, promote stem cell division and proliferation, increase the stem cell proliferation speed and shorten the stem cell culture time; meanwhile, stem cell differentiation can be inhibited, but the differentiation potential of stem cells is not influenced; after the culture medium is used for carrying out passage on the stem cells for multiple times, the stem cells can still keep the original biological characteristics and multidirectional differentiation potential.

The invention discloses a medicinal composition comprising albiflorin and arctiin, wherein the weight ratio of the albiflorin to the arctiin is (0.05 to 0.5):1. The medicinal composition disclosed by the invention can be used for synergically treating the diabetes mellitus by oral administration, particularly can enable diabetics to be recovered and gets rid of the defect that the diabetics need to take medicine for life in the treating process.

The invention relates to a compound recipe kidney-strengthening tablet for enlivening the spleen and strengthening kidney in the traditional technique field. The preparing method comprises the following steps: disintegrating rhubarb horsetails into the powder; collating after immersing; collecting the collation liquid; disintegrating radix salvia miltiorrhiza, astragalus root, extractum glycyrrhizae liquidum and hirudo into the powder; extracting with alcohol reflux; filtering; merging the filtering liquid and the collation liquid; mixing uniformly; concentrating into the dense plaster; getting the extract of five tastes medicine; adding fermenting Cordyceps sinensis bacterial powder, crystalline cellulose, sodium carboxymethylstarch, starch and dolomol; mixing uniformly; getting the product by pelleting. The invention can reduce BUN and SCr level, which improves plasma protein content, improves the nutrient state, accelerates the renovation and the reactivation of nephric tubule epithelial cell, corrects the metabolic disorder of amino acid, protein and lipid, adjusts the cell immunity, improves the immunity, protects and recovers the kidney function, and prevents secondary infection.

The invention discloses an inflammation-diminishing and skin-reproducing oil gauze for treating diabetic feet and a preparation method of the oil gauze. The oil gauze is prepared from the traditional Chinese medicines (coptidis rhizome, cortex phellodendri, scutellaria baicalensis, sanguisorba officinalis and angelica sinensis), which are relatively effective on the diabetic feet in combination. An experiment proves that the inflammation-diminishing and skin-reproducing oil gauze is good in curative effect, free of drug tolerance, free of adverse reaction, economical, practical, convenient to use, and easy to popularize; the compliance and the curative effect on treatment of the diabetic feet can be improved; and the problems of high investment, expensive consumable items, high technical requirements, large economic burden on patients, and poor compliance in application of expensive dressing and closed negative pressure treatment are avoided.

The invention discloses a nutrient solution for bryophytes and a rapid propagation method of the bryophytes. The nutrient solution comprises 1-5 parts by mass of a macroelement fertilizer, 0.1-0.5 part by mass of plant hormone and 1000 parts by mass of water, wherein the macroelement fertilizer is used for providing macroelements for bryophytes. The nutrient solution can break dormancy, accelerateproliferation and shorten the breeding period, so that the purpose of rapid propagation is achieved. The rapid propagation method comprises the following steps: evenly spreading propagules of a bryophyte in a seedling breeding device, laying a cultivation medium in the seedling breeding device, carrying out thorough watering and spraying the nutrient solution; when the propagules grow to seedlings, spraying the nutrient solution and applying illumination; and when the seedlings grow to adult seedlings, spraying the nutrient solution once every 15-30 days, and applying illumination, wherein the nutrient solution is the nutrient solution for the bryophytes. The method is simple in steps and short in carpet formation time, and after a carpet is formed, the structure is stable and the crusting performance is strong, so that the waste of bryophyte in an application is avoided.

The invention discloses a culture medium for obtaining mesenchymal stem cells and exosomes thereof and a preparation method of the culture medium. DMEM/F12 is used as a basic culture medium, and the following components are added: 10-22 g/mL of a serum substitute, 20-55 ng/mL of a growth promoting factor, 5-10 ng/mL of a muscle cell repair factor HCGF, 5-16 mM of an anti-aging factor, a buffer agent, 0.5-3 mg/L of an antioxidant, 1-20 mg/L of vitamins, antibiotics, 5-65 mg/L of trace elements and 1-16 [mu] g/mL of a protective agent. The mesenchymal stem cell culture medium is definite in component, free of serum components, controllable in quality and capable of being produced on a large scale, stem cells can normally grow when the mesenchymal stem cell culture medium is used for in-vitro culture of the stem cells, moreover, the mesenchymal stem cell culture medium is beneficial to division and proliferation of the stem cells, the stem cell amplification rate is greatly increased, and the mesenchymal stem cell culture medium is suitable for large-scale production. Compared with the prior art, the stem cell culture medium has the advantages that stem cell culture time is shortened, and stem cells still can keep original biological characteristics and multidirectional differentiation potential after being subjected to multiple passage times by the aid of the stem cell culture medium.

The invention provides moisturizing and skin tendering essence. The moisturizing and skin tendering essence comprises, by weight, 1-100 parts of recombinant fibroin, 1-100 parts of a radix paeoniae alba extract, 1-100 parts of a rhizoma bletillae extract, 1-100 parts of a herba portulacae extract, 1-100 parts of a honeysuckle flower extract, 1-100 parts of a tangerine fruit extract, 1-100 parts of a red clover extract, 1-100 parts of a papaya fruit extract, 1-100 parts of a royal jelly extract and 1-100 parts of a wheat germ extract. The moisturizing and skin tendering essence has excellent moisturizing, moistening, skin tendering, anti-wrinkle, whitening and anti-allergic effects, and can prevent external irritation to the skin and consolidate the curative effect. The invention also provides a preparation method of the moisturizing and skin tendering essence. The moisturizing and skin tendering essence can play a drug effect to the greatest extent, and the moisturizing, skin-tendering and anti-aging curative effects are improved.

The present ivnention relates to the method of promoting haemtococcus pluvialis multiplication to produce pure strain in a controllable all-weather closed large-scale photobiological reactor system. The system has main reactor made of transparent material, outside controllable lighting facility, speed regualting vane wheel stirrer, water rolling wheel, bacteria-free ventilator, oxygen system, salty and fresh water nutritious liquid compounding and temperature controlling system, cleaning and sterilizing system. It can speed up the multiplication of haemococcus pluvialis, shorten the culture period and obtain pure strain.

The invention provides a method for improving the human in-vitro fertilization embryo development rate. The method is characterized in that an in-vitro fertilization culture solution is prepared by adding a traditional Chinese medicine monomer in a human oocyte conventional culture solution to improve the human in-vitro fertilization embryo development rate, wherein the traditional Chinese medicine monomer is any of baicalin, icariin and ligustrazine, and the mass ratio (or volume ratio) range of the traditional Chinese medicine monomer and the human oocyte conventional culture solution is 6.0ug/mL, 2.0 ug/mL and 3.0 ug/mL. The method provided by the invention has the benefits that studies show that the fertilization culture solution prepared by adding the baicalin as the traditional Chinese medicine monomer in the conventional culture solution has a good promoting effect on human in-vitro fertilization embryo development and is better than a control group without a traditional Chinese medicine ingredient in aspects of cleavage rate, blastocyst development rate and the like.

The invention discloses a serum-free adipose-derived stem cell culture medium and a culture method of adipose-derived stem cells (ADSCs). The culture medium comprises a basic culture medium body and additives, the basic culture base body is a DMEM (dulbecco's modified eagle medium)/F12, the additives include transferrin, fibroblasts growth factors, C-algocyan, acanthopanax senticosus saponins B, scabiolide-3-O-glucosides and beta-ecdysterone. By the arrangement, the growth and proliferation of the ADSCs can be promoted, the damage of proliferation activity of the ADSCs during culture can be reduced, and thus, the in-vitro amplification efficiency of the ADSCs is greatly improved.