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Method for improving haematococcus pluvialis multiplication to produce pure strain in photo-biological reactor

A photobioreactor, Haematococcus pluvialis technology, applied in the direction of photobioreactor, stress-stimulated microbial growth, support/immobilization of microorganisms, etc., can solve the problem of low population density, low yield per unit area, biological Pollution and other problems, to achieve the effect of accelerating growth and proliferation and shortening the expansion time

Inactive Publication Date: 2005-11-09
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main factors hindering the large-scale production of Haematococcus pluvialis are the high cost caused by the low population density of Haematococcus pluvialis culture process and the low yield per unit area.
Haematococcus pluvialis is cultivated in small-scale bioreactors or airlift bioreactors composed of pipeline photobioreactors or small immobilized systems. Decreased light transmittance and difficult to clean. Although different types of paddle wheels have been used to stir and mix to minimize the shading effect between algae cells and improve the utilization of light, it is only suitable for small-scale experiments in the laboratory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017]Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) is 500×150×65cm in length, width and depth. The bottom and surroundings are made of 2cm thick colorless and transparent tempered glass, and the cover part is made of 0.1mm thick 26 100W solar lamps with adjustable brightness and 18 40W energy-saving spotlights are installed around the main reactor and up and down; The rotation speed of the scroll wheel is 50r / m, and the brackish and fresh water nutrient solution circulates at a low rate of 0.04m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with 3 liquid storage tanks with a capacity of 4000L, and the agitator rotates at a speed of 80r / m. The composition of brackish water nutrient solution: 65% clear water, 35% seawater, 0.21g / L potassium nitrate, 0.52g / L sodium acetate, 0.04g / L magnesium sulfate, 0.04g / L potassium dihydrogen phosphate, 0.1g / L urea; Clear wa...

Embodiment 2

[0019] Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) has a length, width, and depth of 220×110×55cm. The bottom and surrounding areas are made of colorless and transparent tempered glass with a thickness of 1.5cm. The thickness of the cover is 0.06 mm transparent film and sealed firmly with adhesive tape, 20 40W fluorescent lamps with adjustable brightness are installed around and up and down the main reactor; the rotational speed of the speed-regulating paddle impeller in the reactor is 20r / m; the rotational speed of the water roll is 30r / m m, the brackish and fresh water nutrient solution circulates at a low rate of 0.02m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with 3 liquid storage tanks with a capacity of 800L, and the agitator speed is 60r / m. The composition of brackish water nutrient solution: 40% clear water, 60% seawater, 0.2g / L potassium nitrate...

Embodiment 3

[0021] Establish an all-weather closed large-scale operation system photobioreactor. The main reactor (water tank) is 200×100×65cm in length, width and depth. The bottom and surrounding areas are made of 1cm thick colorless and transparent tempered glass, and the cover part is made of 0.2mm thick The transparent film is sealed with an adhesive, and the power of adjustable brightness is installed around the main reactor and up and down. The power is 7 40W fluorescent lamps and 5 100W solar lamps; The rotation speed is 10r / m, and the brackish and fresh water nutrient solution circulates at a low rate of 0.03m / s; the brackish and fresh water nutrient solution deployment and temperature control system is equipped with 2 liquid storage tanks with a capacity of 800L, and the agitator rotates at a speed of 20r / m. Fresh water nutrient solution composition: 25% clear water, 75% seawater, 0.4g / L potassium nitrate, 0.4g / L sodium acetate, 0.3g / L urea, 0.04g / L magnesium sulfate; clean water...

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Abstract

The present ivnention relates to the method of promoting haemtococcus pluvialis multiplication to produce pure strain in a controllable all-weather closed large-scale photobiological reactor system. The system has main reactor made of transparent material, outside controllable lighting facility, speed regualting vane wheel stirrer, water rolling wheel, bacteria-free ventilator, oxygen system, salty and fresh water nutritious liquid compounding and temperature controlling system, cleaning and sterilizing system. It can speed up the multiplication of haemococcus pluvialis, shorten the culture period and obtain pure strain.

Description

technical field [0001] The invention relates to a method for using a bioreactor to promote cell division and proliferation of Haematococcus pluvialis to increase the number of algae and obtain pure algae species. Background technique [0002] Haematococcus pluvialis is a single-celled green algae with oval or oval cells, ranging in size from about 5 microns to 20-30 microns, sometimes even larger. There are two equal-length flagella, and the swing of the flagella pushes the cells to swim freely; there is a gap between the cell wall and the protoplast, which is filled with gelatinous substances, and there is a cytoplasmic filament connection between the cell wall and the protoplast, and the vegetative reproduction is cell division, forming 2, 4 , 8, 16 or even more daughter cells, asexual reproduction forms glue groups or thick-walled spores when the environment is unfavorable. Haematococcus pluvialis cells in the vigorous growth stage are gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12M1/42C12N1/12
CPCC12M31/02C12M21/02C12M41/12C12M39/00C12M27/02C12M37/00
Inventor 刘昕袁建平王江海黄晓霓
Owner SUN YAT SEN UNIV
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