6052results about "Apparatus sterilization" patented technology

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Described herein are systems and methods for automated cell transduction within a cell processing system. A system for cell processing may include a cell processing cartridge having a transduction system. The transduction system may include a fluidic manifold, one or more modules for performing a cell processing protocol, and a tube having a surface area to volume ration of between about 1,260 mm2 / mL and about 5,080 mm2 / mL. A method for cell processing may first include flowing cells through a tube of a flow cell of a cell processing cartridge for a first time period to achieve a transduction efficiency of at least 50%. Second, the method may include expanding the cells within a bioreactor module of the cell processing cartridge for a second time period.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

This disclosure relates to a biological tissue forming method includes the following steps: providing a biological tissue forming package with a base, a membrane, a sliding assembly and a sealing film that seals a chamber of the base used for receiving the membrane and the sliding assembly with one of the base and the sealing film being light-transmitting; passing a biological tissue fluid through the sealing film and the sliding assembly so as to be dispensed on the membrane; moving the sliding assembly away from a covering position used for covering the membrane via at least one passive magnetic element so as to expose the biological tissue fluid on the membrane; and emitting the biological tissue fluid exposed on the membrane by a curing light transmitting through the one of the base and the sealing film so as to cure the biological tissue fluid into a biological tissue.

There has been demand for an additional method for producing antibodies. The present invention provides: a polymer-coated crosslinked alginate gel fiber in which a core layer containing a crosslinked alginate gel and either antibody-producing cells (e.g., antibody-producing CHO cells) or bioactive-substance-producing cells (e.g., MIN6 cells derived from pancreatic β cells) is coated with a cationic polymer; and a method for producing antibodies, a bioactive substance, etc., using the fiber.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

Provided is a novel cooling tower for xylanase preparation, comprising a water tank platform, a tower body, and support columns, wherein a circular ring block is rotatably connected to the inner wall at the bottom of the water tank platform, an annular cavity is formed along the inner wall of the circular ring block, a plurality of straight rods are evenly distributed in, penetrate through, and are slidably connected to the inner wall of the annular cavity, a scraper is fixedly connected to one end of each straight rod, and the scrapers slidably abut against the inner wall at the bottom of the water tank platform; and a power mechanism for driving the scrapers to clean impurities inside the water tank platform is arranged inside the annular cavity. An annular rack, a plurality of gears, the straight rods, and a scraper structure are provided, the output end of a motor drives rotating shafts fixedly connected thereto to rotate by a certain angle, and by means of the gears, the annular rack, and a toothed belt structure, the rotating shafts drive the straight rods and the scrapers to slide a certain distance, so that the scrapers scrape some impurities remaining on the inner wall at the bottom of the water tank platform, until these impurities are scraped and accumulated in a circular platform hole, and then discharged to the outside for collection.

A reinforcing and scaling construction for a bioprinted tissue model, which comprises a casing and sealing bioink, the casing comprising an inner module comprising a casing base (1) and a perforated cover (2), and an outer module comprising a container (3) comprising technological valves (5), an outer cover (4) and plugs (6) for technological valves (5). The invention also relates to a method for assembling the reinforcing and sealing construction.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

A disposable kit for a bioprocessing system includes a tray having a spine having a first window and a second window, at least one bioprocessing vessel received within the tray, a pinch valve manifold removably received by the spine within the first window, a first tubing segment removably received by the spine within the second window, and a second tubing segment removably received by the spine within the second window.

The invention discloses a bacteriophage reagent production filtering device facilitating impurity cleaning, and relates to the technical field of filtering devices.The bacteriophage reagent production filtering device comprises an impurity cleaning mechanism and a collecting frame installed at the bottom of the impurity cleaning mechanism, a mixing mechanism is arranged at the top of the impurity cleaning mechanism, and a feeding pipe is arranged at the top of the mixing mechanism; the impurity cleaning mechanism comprises a filter box, a centrifugal motor is fixedly installed on one side of the middle of the filter box, the output end of the centrifugal motor is fixedly connected with a rotating block, a first rotating support is fixedly installed on one side of the rotating block, and rotating discs are symmetrically installed at the two ends of the first rotating support; a plurality of connecting sliding grooves are formed in the rotating discs, a plurality of first filter screen plates are connected between the two rotating discs in a sliding mode, full filtration of a phage reagent can be achieved by arranging the multiple sets of first filter screen plates, and impurities subjected to preliminary filtration can be filtered for the second time through the centrifugal effect of the multiple sets of first filter screen plates.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

A device for modelling physiological and pathophysiological states of the brain is provided. The device comprises a hydrogel with micropattern channels having a length, width and depth. The micropattern channels comprise cells selected from the group consisting of neurons, astrocytes, microglia, oligodendrocytes, neuronal organoids, cancer cells, cancer spheroids, brain tumoral cells and combinations thereof.

The invention discloses a fermentation device for traditional Chinese medicine decoction piece production, and belongs to the field of traditional Chinese medicine fermentation. A fermentation device for producing traditional Chinese medicine decoction pieces comprises a fermentation tank and further comprises a controller fixedly arranged at the bottom of the fermentation tank; the sealing cover is in threaded connection with the top of the fermentation tank; the rotating shaft is rotationally arranged on the sealing cover; the motor is fixedly arranged at the top of the fermentation tank, and the output end of the motor is fixedly connected with the rotating shaft; the hemispherical head is fixedly arranged at the bottom of the rotating shaft; the limiting groove is formed in the hemispherical head; the rotating ball is rotationally arranged in the hemispherical head, and a limiting block matched with the limiting groove is fixedly connected to the rotating ball; according to the device, the mutual contact efficiency of raw materials is improved, the situation that the temperature of the raw materials on the inner wall of the fermentation tank is high after long-time heating is effectively prevented, meanwhile, the heating uniformity of the raw materials in the fermentation tank is effectively improved, and then the fermentation efficiency of traditional Chinese medicine is effectively improved.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture tank device which comprises a culture tank, a concave observation area is arranged in the center of the top of the culture tank, a central culture area surrounding the observation area is arranged in the culture tank, an oil path temperature control area wraps the culture tank, and a double-helix heat conduction oil path is integrated in the oil path temperature control area. Through the cooperation of the uniform distributor and the distributed simulated liquid conveying unit, the gas-liquid proportion and fluid distribution can be accurately regulated and controlled, the physical and chemical conditions in the deep sea or extreme environment are simulated, the constant-temperature precision of + / -0.005 DEG C is realized through the double-helix heat conduction oil way, the low-temperature environment is stably maintained, and the real thermodynamic conditions of deep-sea sediments are simulated; the device meets the sensitive requirements of different microorganisms on temperature, and has the characteristics of high practicability and capability of simulating a dynamic fluid environment of deep sea.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

The organ-like 3D printer temperature control system comprises a first liquid guide assembly, a first driving assembly, a pore plate and a first temperature control assembly. The first liquid guiding assembly is provided with a first liquid guiding end used for guiding out biological ink. The first driving assembly is connected to the first liquid guiding assembly and used for driving the first liquid guiding assembly to move. The pore plate is arranged below the first liquid guide assembly, the pore plate comprises an upper surface wall and a lower surface wall, the upper surface wall is concavely provided with a plurality of accommodating grooves, and each accommodating groove is used for accommodating the biological ink guided out by the first liquid guide end. The first temperature control assembly is attached to the lower surface wall and used for adjusting the temperature value of the pore plate to a first preset range. According to the invention, the accommodating grooves are integrated on the pore plate, and the temperature of the solution in each accommodating groove can be mutually conducted through the pore plate, so that the temperature of each accommodating groove can tend to be average, and operations such as large-batch printing and culturing of the organoids can be more efficiently and accurately carried out.

The invention relates to the technical field of biochemical engineering and food processing, in particular to a fish collagen extraction process which comprises the following steps: S100, raw material pretreatment; s200, carrying out crushing and enzymolysis pretreatment; s300, carrying out an enzymatic hydrolysis reaction; s400, carrying out solid-liquid separation; s500, carrying out concentration treatment; and S600, drying treatment is carried out. The advanced spectrum analyzer is used for accurately determining the freshness of the fish body, the automatic cutting and ultrasonic cleaning technologies are combined, the quality of raw materials is effectively guaranteed, the enzymolysis reaction conditions are strictly controlled through the high-precision metering pump and the heat exchange system, the enzymolysis efficiency and the product quality are improved, and the production cost is reduced. By adopting a multi-layer composite membrane structure and a specific concentration technology, the efficiency and purity of solid-liquid separation and concentration treatment are remarkably improved, the production process is optimized, the energy consumption and the cost are reduced, pollutants in the production process are effectively removed through application of a plasma cleaning and air purification system, and the product quality is improved. And reliable guarantee is provided for production of high-quality fish collagen products.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The invention relates to the technical field of fruit and vegetable fermentation polyphenol extraction, and discloses a fruit and vegetable fermentation polyphenol extraction device and method.The fruit and vegetable fermentation polyphenol extraction device comprises a reaction tank, the reaction tank is used for fruit and vegetable fermentation polyphenol extraction, and a fermentation extraction control mechanism is rotationally arranged in the middle of the reaction tank; the device comprises a reaction tank, the top end of the reaction tank is fixedly provided with a positioning shaft, one side of the reaction tank is fixedly provided with a fermentation extraction lifting control mechanism, the bottom end of the fermentation extraction lifting control mechanism is fixedly provided with a rotation control mechanism, and the output end of a first motor fixed to the top end of the reaction tank drives the positioning shaft to rotate rapidly in the forward direction; a rotary aeration effect is achieved at the bottom end of the fruit and vegetable liquid in cooperation with rotation of the communicating frame, so that fruit and vegetable fermentation liquid and air are fully mixed, it is ensured that microorganisms in the fruit and vegetable liquid can obtain enough oxygen to conduct normal metabolic activity, and an extracting solution enters the inner wall of the reaction tank through an extracting solution feeding pipe by adopting the same method; the extracting solution and the fruit and vegetable liquid are fully mixed by alternately pumping the extracting solution and the gas.

The invention generally relates to devices for manufacture of tissue products, and, more particularly, to material layering assemblies for processing tissue layers to produce a multi-layered tissue product. The invention provides a material layering system to support and / or hold various layers of material to form a multi-layered product.

A fully automated chemiluminescence immunoassay analyzer, including a sample and reagent receiving device for receiving a sample and a reagent, a dispensing device for aspirating and discharging the sample and the reagent, a mixing device for mixing the sample and the reagent in a reaction vessel, an incubation and luminescence detection device for incubation and luminescence detection, a magnetic separation cleaning device for separation cleaning an analyte and impurities in the reaction vessel, a reaction vessel grasping device for transferring the reaction vessel, and a liquid path device.

A microfluidic device, a microfluidic system comprising said microfluidic device, and to a method of culturing biological material.

The automatic feed adjusting type vacuum rotary drum comprises symmetrically-arranged supporting seats, a material storage groove, a rotary drum structure and a spraying structure are arranged between the symmetrically-arranged supporting seats, a vacuum pump and a transmission structure are arranged on the two sides of the rotary drum structure respectively, and an adjusting material scraping structure, a material supplying and pressing structure and a material guiding structure are arranged on the material storage groove. And a plurality of groups of camera structures are arranged on the wall body close to one side of the adjusting scraping structure. By the adoption of the structure, the whole-process automatic monitoring and adjusting of the material treatment process are achieved, the efficiency and accuracy of screening, cleaning and finish machining are remarkably improved, it is ensured that machining parameters are adjusted in time, material accumulation or loss is avoided, and therefore the continuity and stability of the machining process are kept; due to the design of a multi-layer filtering structure, impurities are removed in a layered mode according to material characteristics, especially when high-humidity materials are treated, the screen mesh can be effectively prevented from being blocked, and the high quality of the treated materials is guaranteed.

An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

The invention relates to the technical field of stem cell culture, and one embodiment of the invention provides a stem cell differentiation culture device which comprises a culture box and further comprises an air inlet pipe, a main fan, a fixed box, a conversion cylinder, a filter cylinder, an auxiliary fan, a discharge box and a cleaning mechanism, the culture box is communicated with a temperature adjusting system through the air inlet pipe, the fixed box is communicated with the air inlet pipe, and the air inlet pipe is communicated with the main fan. The conversion cylinder is rotatably arranged in the fixed box, the interior of the conversion cylinder is divided into two conversion cavities, the filter cylinder is fixedly connected to the interior of the conversion cavities and communicates with the air inlet, the auxiliary fan is installed in the filter cylinder, the discharge box communicates with the fixed box through the discharge port, and the discharge box communicates with a discharge pipe. The cleaning mechanism is used for being matched with the auxiliary fan to clean the inner wall of the filter cartridge. By means of the technical scheme, the technical problem that dust accumulates on a filter screen in a ventilation system in the prior art, and regular disassembly and cleaning are needed is solved.