2182results about "Apparatus sterilization" patented technology

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

A device and method for assembling objects by generating an axial acoustic radiation force in order to position the objects on pressure nodes and / or antinodes and generating a transverse acoustic radiation force in order to move the thus positioned objects on a porous membrane contact structure.

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention relates to the technical field of fishery breeding tail water treatment, and discloses circulating fishery tail water purification equipment based on photosynthetic microorganisms, the circulating fishery tail water purification equipment comprises a photosynthetic reaction treatment box and a biomass collection box, an LED light source module and a lamp tube surface cleaning assembly are arranged in the photosynthetic reaction treatment box, and a waste gas collection and circulation assembly is arranged at the top of the photosynthetic reaction treatment box; an inclined plate type grating plate and a grating plate impurity scraping assembly are arranged in the biomass collecting box, and an inner circulation and outer discharge assembly of photosynthetic microorganisms is arranged at the bottom of the biomass collecting box. The lamp tube surface cleaning assembly conducts multi-dimensional cleaning on the surface of a light source through linear and rotary composite motion, and the illumination efficiency is guaranteed; carbon-rich waste gas is recycled through the waste gas collecting and circulating assembly, dehumidified and then re-injected into the water body, and carbon source circulation is achieved; the grating plate impurity scraping component and the internal circulation component are used for regularly cleaning the blockage of the grating and reflowing high-activity strains, so that the stable operation of the system is maintained, and the purification efficiency is improved.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

Systems and methods of clarifying a liquid suspension at high speeds contained in a flexible bag is provided. The method includes nesting at least one flexible bag containing the liquid suspension within at least one bag adapter with the at least one inlet line and / or outlet line extending from the flexible bag to an exterior of the bag adapter. The method further includes at least partially enclosing the at least one inlet line and / or outlet line within a crown cavity defined by a crown assembly. The method further includes centrifuging the at least one bag adapter and the at least one flexible bag containing the liquid suspension in a centrifuge to produce a super-natant fluid and a pellet in the at least one flexible bag.

The invention discloses a culture device for improving polycystic ovarian syndrome lactobacillus reuteri, and belongs to the technical field of lactobacillus reuteri culture.The culture device comprises a working frame, the inner side of the working frame is fixedly connected with a feeding mechanism used for conveying, one side of the feeding mechanism is fixedly connected with a culture mechanism, and the other side of the feeding mechanism is fixedly connected with a conveying mechanism; a liquid feeding mechanism is fixedly connected to the rear side of the feeding mechanism, a supporting mechanism is fixedly connected to the position, located between the feeding mechanism and the culture mechanism, of the inner side of the working frame, an auxiliary ladder is fixedly connected to the surface of one side of the working frame, and a protection frame is fixedly connected to the surface of the auxiliary ladder. On the basis of realizing auxiliary culture of lactobacillus reuteri, strain functions and metabolites can be accurately regulated and controlled, and the delivery problem in PCOS treatment can be solved.

The invention belongs to the technical field of waste resource utilization, and particularly relates to a composite carbon source as well as a preparation method and a special device thereof. The special device for preparing the composite carbon source comprises a crushing device; a production device; a filtering device; a transportation device; and a discharging tank. The method for preparing the composite carbon source by using the special device for preparing the composite carbon source comprises the steps of feeding, production and separation in a starting stage and the steps of feeding, production and separation in a production stage. The production and solid-liquid separation are enhanced by assisting differential ultrasound, so that the production efficiency and the solid-liquid separation effect of the composite carbon source are improved, the material and energy consumption is reduced, the operation is simple, the cost is low, and the corrosivity is small.

The present invention relates to vessels for use as bioreactor, and reactor systems or apparatuses including such vessels. The vessels comprise a tank comprising a plastic or first cured resin material which is biocompatible and which can withstand steam sterilization.

The invention relates to the technical field of fermentation equipment, and discloses hyaluronic acid fermentation production synthesis fermentation equipment and a fermentation technology.The hyaluronic acid fermentation production synthesis fermentation equipment comprises a base plate, and a base is fixedly connected to the middle of the top end of the base plate; a control table is fixedly connected to one side of the front end of the top of the base plate, and a PLC display controller is arranged in the middle of the top end of the front side of the control table. The adaptive stirring mechanism is arranged in the middle of the top end of the base plate and is used for performing corresponding stirring treatment according to different fermentation processes of hyaluronic acid. By adding and arranging the dynamic material supplementing mechanism, in the synthetic fermentation process of hyaluronic acid, the mechanism can detect the precursor concentration in real time, accurately match precursor requirements of different stages of hyaluronic acid fermentation, preferentially supply a carbon source to support thallus growth in the early stage, dynamically adjust the carbon-nitrogen ratio in the middle stage and maintain balance of two precursors, and can be used for preparing the hyaluronic acid. The material supplementing rate is finely adjusted in advance according to the consumption trend in the later period, and synthesis interruption caused by precursor unbalance is avoided.

The invention discloses continuous fermentation equipment for compound fermented sauce, and particularly relates to the technical field of food fermention.The continuous fermentation equipment comprises a support, a hydrolysis tank, a fermentation tank and an after-ripening tank are installed on the support from top to bottom in a stepped mode, and the hydrolysis tank and the fermentation tank are communicated through a first spiral pump; the fermentation tank is communicated with the after-ripening tank through a second screw pump. According to the continuous fermentation equipment for the compound fermented sauce, the dynamic extraction circulating device is arranged, flavor precursor substances can be actively extracted, subjected to ultrasonic wall breaking and subjected to membrane filtration and concentration from main fermented mash and then directly conveyed to the after-ripening tank, high-concentration flavor essence is provided for the after-ripening stage, after-ripening reactions such as esterification are greatly promoted, and the quality of the compound fermented sauce is improved. According to the method, the flavor level of the final product is richer, inhibition on main fermentation is relieved by actively removing small molecule byproducts, flavor precursors are forcibly conveyed to an after-ripening environment, the flavor ripening process is accelerated, and the whole fermentation period is shortened.

The utility model discloses an organic wastewater treatment device capable of recovering biogas, which relates to the technical field of wastewater treatment and comprises a bottom plate, a reservoir is mounted at the top of the bottom plate, a guide frame is fixed on the inner wall of the reservoir, a grating for filtering suspended solids in sewage is slidably connected to the inner wall of the guide frame, and a water outlet is formed in the bottom plate. A cleaning assembly used for dredging the grating is arranged in the water storage tank. The device has the beneficial effects that the filter holes in the grid are dredged through the push block, the grid is prevented from being blocked, the filtering effect is ensured, the pull rope is pulled to drive the collection box to slide on the outer side of the limiting rod, the scraper is driven to scrape and clean the surface of the grid, the floating cover floats up and down along with the biogas generation amount in the sealing tank, and the biogas is collected; the sealing performance between the floating cover and the inner wall of the sealing tank is ensured through the sealing gasket, biogas leakage is prevented, and the collected biogas is recycled through the connecting hose.

The invention relates to the technical field of bioengineering, in particular to an exosome separation and concentration method with marine animal source tissue as a raw material. Comprising the following steps: freeze-drying and crushing a marine animal source tissue sample; performing sample enzymolysis; and removing impurities and concentrating. According to the method disclosed by the invention, the exosome in the cells is fully dissociated through freeze-dried powder crushing and composite enzymolysis processes, and the filtering speed of the sample is accelerated by virtue of ultrasonic oscillation, so that the rapid and high-purity separation and concentration of the exosome in the marine animal source tissue are realized.

The present invention relates to a method for decomposing peracetic acid using a metal compound and a method for culturing microorganisms using the decomposition method. The cultivation of microorganisms using the method of the present invention allows an effective removal of the peracetic acid used in a culture medium for sterilization.

The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.

A three-dimensional (3D) microelectrode array device for in vitro electrophysiological applications includes a substrate and micro vias extending from the bottom face to the top face of the substrate. A microneedle at each micro via extends from the bottom face upward beyond the top face and forms a hypodermic microneedle array on the top face. Metallic traces on the bottom face interconnect the hypodermic microneedles to form the 3D microelectrode array. A microheater is positioned on the bottom face of the substrate. Microfluidic ports may be formed at the substrate. Interdigitated electrodes may be formed at the substrate.

A cell culture device configured to cultivate cells, the culture device includes a plate with at least one well configured to receive at least one cell to be cultivated, a permeable support sheet, a fixation layer, configured to connect the plate to the permeable support sheet, the fixation layer presenting a predefined shape including at least one wall extending between the permeable support sheet and the plate. The fixation layer is designed in accordance with a pre-defined growing path, in order to model and shape the growth of the cultivated cells outside the well in a controlled and predetermined fashion.

The application discloses a process for converting broken hair into methane and relates to the technical field of energy conversion, and comprises the following steps: hair pretreatment, hair crushing and screening, raw material mixing and catalyst adding, anaerobic fermentation reaction, and methane separation and purification. Compared with the prior art, the process has the advantages of rich raw materials, environmental protection, high conversion efficiency, high product quality and strong controllability.

The invention relates to a system for storing or cultivating organ or tissue models and its use. The system is designed for verifying the functionality of the harvested organ or tissue models and allowing them to be processed. The system can be used for cultivating organ and / or tissue models harvested from donors and / or prepared using other techniques, such as using 3D and / or 4D bioprinting or electrospinning techniques, and comprises a chamber (2) for the organ or tissue models, which is equipped with means for measuring and adjusting the temperature of the perfusion liquid in the chamber, a perfusion liquid container (1), which is equipped with means for measuring and adjusting the temperature of the perfusion liquid therein, means (4) for measuring the glucose concentration in the perfusion liquid and adding glucose to the perfusion liquid in a metered amount, means (3) for measuring the pH of the perfusion liquid and adding a substance for adjusting the pH of the perfusion liquid in a metered amount, means for non-contact mixing of the perfusion liquid, and means for measuring and controlling the flow parameters of the perfusion liquid.

The utility model discloses a spore analyzer, which belongs to the technical field of spore analyzers, and comprises an analyzer body, the upper surface of the analyzer body is fixedly connected with four connecting columns, the upper surfaces of the four connecting columns are fixedly connected with a protective cover, the upper surface of the analyzer body is fixedly connected with a filter cartridge, and the filter cartridge is fixedly connected with a filter screen. The filter cartridge is sleeved with a cleaning device, and a blowing device is fixedly connected into the protective cover. According to the utility model, the cleaning operation of the filter cartridge is realized through the synergistic effect of the handheld groove and the push plate, when an operator puts the hand in the handheld groove to apply thrust, the push plate drives the lantern ring and the scraper to move along the axial direction, and the scraper keeps close contact with the outer surface of the filter cartridge, so that particulate matters accumulated on the surface are effectively stripped in the relative movement process; and the specially designed inclined scraper structure can guide the stripped pollutants to the upper surface of the analyzer body to realize directional removal, so that the permeability of the filter cartridge is maintained.

The application relates to the technical field of dairy product processing, and discloses an expansion culture system for dairy product strains, a sterile online mixing device and an expansion culture tank for strain expansion culture; a feeding line is arranged between the sterile online mixing device and the expansion culture tank, an expansion culture tank feeding valve is installed on the feeding line; a heat preservation layer is arranged on the periphery of the expansion culture tank, and an ice water inlet valve is arranged on the heat preservation layer to cool the expansion culture stock solution in the expansion culture tank; a discharging line is arranged at the other end of the expansion culture tank, and a material quantitative discharging valve and a flow controller are arranged on the discharging line. The application has the effects of reducing strain waste in the production process and reducing production cost.

The present invention relates to a mould (1), the mould comprising a receptacle (10) and a lid (20) for forming a tissue graft, such as an autologous tissue graft for transplant and suturing. The mould is adapted to contain layers of biological material, providing pressure to the biological material and drain out excess fluid, thus providing a fast and effective means for building a tissue graft. The mould may comprise one or more resilient members, and at least one or more of the resilient members (RM) may be suitable to provide a specified force to apply the desired pressure to the tissue graft. The invention further relates to a method of using the mould.

This invention belongs to the field of microbial detection, specifically a rapid detection device for pharmaceutical microorganisms. It includes a detection chamber with a base fixedly installed at its bottom. A servo motor is fixedly installed in the middle of the base, and a rotating rod is fixedly installed at the output end of the servo motor. A turntable is fixedly installed at the top of the rotating rod, and placement slots are formed around the turntable. Multiple sets of steel balls are rotatably installed inside these slots, and protective rings are rotatably installed on the outer walls of the slots. This invention uses clamps to fix the culture dishes, preventing them from shifting off-center during rotation. It allows for the detection of multiple culture dishes simultaneously, facilitating control experiments and achieving a convenient and rapid result. It also avoids the problem of multiple handling of individual tests introducing more bacteria into the instrument. Furthermore, sterilization by ultraviolet light before detection greatly reduces the impact of bacteria on the detection process.

A lung cancer cell culture tank with an ozone sterilization function belongs to the field of biotechnology and cell culture equipment, and is characterized in that a sealing door (2) is arranged at the front part of a tank body (1), a 8W UVC ultraviolet lamp tube (3) is mounted at the top in the tank, and a nano titanium dioxide photocatalyst coating is sprayed on the inner wall of the tank body to form an ultraviolet sterilization and ozone management module; the tank bottom wall (18) is provided with a semiconductor chilling plate (5) and a temperature sensor (6); an air filter (7) with a 0.22 mu m hydrophobic filter membrane is arranged at the upper part of the rear wall (11) of the tank body; the semiconductor chilling plate (5), the temperature sensor (6) and the air filter (7) jointly form a cell culture environment maintaining system; a glove hole (4) is formed in the center of each tank body side wall (12) and is connected with an operation glove (17) through a telescopic sleeve (16); the control panel (8) is located on the front face of the tank body. The invention has the advantages of simple manufacture, convenient use, low cost and obvious effect.