8732results about "Apparatus sterilization" patented technology

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A serum-free medium mesenchymal stem cell inoculation device, comprising a base, a rotating assembly, a center rod, a supporting rod, a clamping assembly, a test tube, a connecting tube, an arc-shaped air chamber, a fixing rack, a cooling tube and a collecting assembly, wherein the center rod is arranged at the top of the base through the rotating assembly, the supporting rod is annularly equally spaced on an outer wall of the top of the center rod along an axis of the center rod, the test tube is arranged at an end of the supporting rod away from the center rod through the clamping assembly, the connecting tube is arranged on the test tube, the fixing rack is arranged on an outer wall of the bottom of the center rod, the arc-shaped air chamber is in communication with the connecting tube through the cooling tube, and the collecting assembly is arranged on a side of the base. According to the inoculation device, by means of mutual cooperation of the rotating assembly, a moving assembly, the clamping assembly and the collecting assembly, operation errors caused by confusion of diluted test tubes and undiluted test tubes in a liquid adding and pipetting process of a worker are avoided, thereby improving the working efficiency of the worker.

Described herein are systems and methods for automated cell transduction within a cell processing system. A system for cell processing may include a cell processing cartridge having a transduction system. The transduction system may include a fluidic manifold, one or more modules for performing a cell processing protocol, and a tube having a surface area to volume ration of between about 1,260 mm2 / mL and about 5,080 mm2 / mL. A method for cell processing may first include flowing cells through a tube of a flow cell of a cell processing cartridge for a first time period to achieve a transduction efficiency of at least 50%. Second, the method may include expanding the cells within a bioreactor module of the cell processing cartridge for a second time period.

One or more methods for treating a tissue site comprises collecting a tissue sample that has keratinoytes and placing the tissue sample in a cartridge that has a well plate, positioning the cartridge within a base unit that has a tissue disaggregator, activating the base unit to rotate the well plate to align wells in the well plate with the tissue sample and the tissue disaggregator, operate the tissue disaggregator to separate the tissue sample to form a regenerative epidermal suspension, and provide the regenerative epidermal suspension to a tissue site to enhance healing of the tissue site.

Mammalian cell production systems are disclosed herein wherein a living mammalian cell culture including a mammalian growth medium operative is co-cultured with a living algae cell culture including an algae growth medium operative to feed nutrients and carbon dioxide to a plurality of algae cells and to transport an algae waste from the plurality of algae cells. The living algae cell culture produces an algae waste with one or more nutrients and oxygen operative to feed the mammalian cell culture, while the living mammalian cell culture produces a mammalian waste with one or more nutrients and carbon dioxide operative to feed the algae cell culture. The systems are capable of a continuous operation at normal mammalian temperature and pH, thereby removing a need to cool down the algae growth medium and / or heat up the algae growth medium for a treatment of algae or for a replenishment of algae.

The invention discloses a stem cell culture device and method, and belongs to the field of stem cell culture. A stem cell culture device comprises a culture box and a germicidal lamp fixed to the top of an inner cavity of the culture box, the middle of the inner cavity of the culture box is rotationally connected with a rotating disc, the bottom of the rotating disc is fixedly connected with a U-shaped piece, the other end of the U-shaped piece is fixedly connected with a culture disc, the culture disc is attached to the inner wall of the culture box in a sliding mode, and a culture dish is installed at the top of the culture disc. An adsorption assembly for fixing a culture dish is arranged at the bottom of the culture tray; according to the device, a spraying pipe drives a spraying disc to rotate, so that a nutrient solution is sprayed out in a rotating manner along atomizing nozzles at all positions, and a culture disc can drive culture dishes at all positions to rotate in cooperation with the fitting friction effect between a friction wheel and a friction ring, so that the comprehensive and uniform spraying effect is achieved; the nutrient solution adding efficiency is improved, meanwhile, it is guaranteed that the quality of the nutrient solutions added into the culture dishes is the same, and therefore the culture effect is improved.

A method and system to make a modular microfluidic device in a miniaturized form for cell culture, co-culture, and organ-on-a-microfluidic device as in-vitro model of human organs within compartmentalized 3D structures is described. The modular microfluidic device enables co-culturing of heterogeneous cell assemblies that are adjacent or sequentially connected to promote enhanced cell-cell interaction. In vivo-like flow into and / or from one cell growth chamber to adjacent cell growth chamber is maintained by micro-engineered porous barriers i.e., porous walls and membranes. These porous barriers provide a tool for mimicking the paracrine exchange between cells in the human body. The system comprises of a set of microelectrodes for real-time and long-term monitoring and quantitative measurement of the impact of stimuli on the epithelial tissue permeability. The modular microfluidic system provides a platform technology for bio-fabrication of variety of miniaturized in-vitro models for mimicking the structure and function of single and multi-organ models.

This disclosure relates to a biological tissue forming method includes the following steps: providing a biological tissue forming package with a base, a membrane, a sliding assembly and a sealing film that seals a chamber of the base used for receiving the membrane and the sliding assembly with one of the base and the sealing film being light-transmitting; passing a biological tissue fluid through the sealing film and the sliding assembly so as to be dispensed on the membrane; moving the sliding assembly away from a covering position used for covering the membrane via at least one passive magnetic element so as to expose the biological tissue fluid on the membrane; and emitting the biological tissue fluid exposed on the membrane by a curing light transmitting through the one of the base and the sealing film so as to cure the biological tissue fluid into a biological tissue.

A vegetable anti-floating submerged fermentation tank comprises a tank body, and a sealing cover is arranged at the upper end of the tank body. The lower end of the tank body is communicated with a connecting pipe, the other end of the connecting pipe is connected with a lower three-way valve, one port of the lower three-way valve is connected with a drainage pipe, the other port of the lower three-way valve is connected with a circulating water inlet pipe, the other end of the circulating connecting pipe is connected with a circulating pump, and the circulating pump is connected with a circulating water outlet pipe; the other end of the circulating water outlet pipe is connected with an upper three-way valve, one port of the upper three-way valve is connected with a water inlet pipe, the other port of the upper three-way valve is connected with a water inlet main pipe, the other end of the water inlet main pipe is connected to the upper end of the tank body, and a plurality of floating plates are arranged in the tank body in the vertical direction; according to the invention, the fermentation environment of large-batch vegetable fermentation is improved, and during fermentation, adverse effects of external impurities, infectious microbes, oxygen and the like on fermentation can be avoided.

There has been demand for an additional method for producing antibodies. The present invention provides: a polymer-coated crosslinked alginate gel fiber in which a core layer containing a crosslinked alginate gel and either antibody-producing cells (e.g., antibody-producing CHO cells) or bioactive-substance-producing cells (e.g., MIN6 cells derived from pancreatic β cells) is coated with a cationic polymer; and a method for producing antibodies, a bioactive substance, etc., using the fiber.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

The invention belongs to the technical field of microorganisms, and particularly relates to a microorganism detection culture device and method.The system comprises an intelligent environment control system, and a sensor monitors the temperature, humidity and gas concentration of a culture environment in real time to ensure that environment parameters needed by microorganism growth are kept in the optimal state; the automatic sample processing and operating system avoids pollution or errors caused by manual intervention through a mechanical arm or an automatic transmission device; the real-time image monitoring and analyzing system is used for monitoring the growth state of the microorganisms in the culture dish; the camera can continuously shoot images in the culture process; the rapid detection module rapidly obtains activity information of microorganisms, rapid detection is achieved, and time needed by traditional culture is shortened. According to the invention, accurate control, automatic operation and rapid detection are realized, and an efficient, safe and intelligent solution is provided for microbial culture and detection.

The invention relates to the technical field of microorganism sampling equipment, in particular to an ore-containing salt lake brine microorganism sampling device and method.The ore-containing salt lake brine microorganism sampling device comprises a balance weight floating platform, and through cooperation of a laser ranging mechanism, a PLC, a first electromagnetic valve, a second electromagnetic valve and an air pump, the sampling depth can be adjusted according to the actual sampling depth requirement; sampling distance parameters are flexibly set through the PLC according to actual sampling requirements, the winding mechanism is controlled to work through the PLC, the winding mechanism drives the sampling head to be lowered or pulled back, the distance between the sampling head and the bottom plate is detected through the laser ranging mechanism, and when the actual distance detected by the laser ranging mechanism reaches a set value, the sampling head is driven to move back and forth. The first electromagnetic valve and the second electromagnetic valve on the to-be-sampled barrel are started through the PLC, the air pump is started, a sample enters from the sampling head through an air pumping mode of the air pump, the operation is simple, the use is convenient and fast, and meanwhile, the sampling flexibility of samples with different depths is improved.

Provided is a novel cooling tower for xylanase preparation, comprising a water tank platform, a tower body, and support columns, wherein a circular ring block is rotatably connected to the inner wall at the bottom of the water tank platform, an annular cavity is formed along the inner wall of the circular ring block, a plurality of straight rods are evenly distributed in, penetrate through, and are slidably connected to the inner wall of the annular cavity, a scraper is fixedly connected to one end of each straight rod, and the scrapers slidably abut against the inner wall at the bottom of the water tank platform; and a power mechanism for driving the scrapers to clean impurities inside the water tank platform is arranged inside the annular cavity. An annular rack, a plurality of gears, the straight rods, and a scraper structure are provided, the output end of a motor drives rotating shafts fixedly connected thereto to rotate by a certain angle, and by means of the gears, the annular rack, and a toothed belt structure, the rotating shafts drive the straight rods and the scrapers to slide a certain distance, so that the scrapers scrape some impurities remaining on the inner wall at the bottom of the water tank platform, until these impurities are scraped and accumulated in a circular platform hole, and then discharged to the outside for collection.

A reinforcing and scaling construction for a bioprinted tissue model, which comprises a casing and sealing bioink, the casing comprising an inner module comprising a casing base (1) and a perforated cover (2), and an outer module comprising a container (3) comprising technological valves (5), an outer cover (4) and plugs (6) for technological valves (5). The invention also relates to a method for assembling the reinforcing and sealing construction.

The invention relates to the technical field of biological tissue engineering, and discloses a nerve-blood brain barrier co-culture model, which comprises a culture chip, and nerves and blood brain barriers respectively constructed in upper culture micropores and lower culture micropores of the culture chip. Wherein a semi-permeable membrane is arranged between the lower-layer culture micropores and the upper-layer culture micropores of the culture chip; the nerves are obtained by inoculating neurons / brain organs obtained by stem cell differentiation into the upper culture micropores for co-culture, and the blood brain barrier is obtained by inoculating brain microvascular endothelial cells obtained by stem cell differentiation into one side of the semipermeable membrane in the lower culture micropores for co-culture. The co-culture model is higher in biomimetic property, and can better simulate the structure and function of the blood brain barrier, for example, the co-culture model has a barrier function closer to the physiological level, the selective permeability of a small molecule drug is matched with the in-vivo permeation condition, and the co-culture model has the function of a small molecule related transporter. The invention further discloses a construction method and application.

The invention discloses a needle mushroom bacterial liquid culture reaction kettle, and relates to the technical field of bacterial liquid culture preparation equipment.The needle mushroom bacterial liquid culture reaction kettle comprises a rotating assembly, the rotating assembly is fixedly connected with a motor, the outer side of the rotating assembly is fixedly connected with a round pipe, square holes are formed in the outer side of the round pipe, and the square holes are evenly distributed with the round pipe as the center; a fixing ring is rotatably connected to the end, away from the rotating assembly, of the circular pipe, a rotating plate is fixedly connected to one end of the fixing ring, a supporting rod is fixedly connected to the end, close to the rotating plate, of the fixing ring, a reset spring is fixedly connected to the end, close to the circular pipe, of the supporting rod, and a baffle is slidably connected to the outer side of the supporting rod. According to the flammulina velutipes bacterial liquid culture reaction kettle, a culture solution and strains in the tank body are stirred in multiple degrees of freedom and different directions through rotation of the round pipe and the rotating plate, so that the strains and the culture solution are uniformly mixed, and the quality of culture production of the strains is ensured.

The invention belongs to the technical field of cell culture, and particularly relates to an intelligent chimeric cell culture cabin which is characterized by comprising an outer cabin body and a culture mechanism mounted above the outer cabin body, the fan rotates to suck air outside the device into the culture cabin through the air inlet, and in the process of blowing the air into the culture cabin, the heating pipe can heat the sucked air to increase the temperature of the air, so that the temperature of the air sucked into the device adapts to the temperature required by cell culture, and the situation that cell culture is affected by improper temperature is avoided; the sucked air is discharged out of the device through the air outlet after being circulated in the culture cabin body, so that the air in the culture cabin body can be circulated, meanwhile, waste gas generated by culturing cells in the culture cabin body is taken away, and angle openings of the air dissipation openings are staggered, so that the air discharged from the air dissipation openings can face different angles, and the air dissipation effect is improved. And the situation that the blown airflow faces the same angle and is too intense is avoided.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The utility model provides a cell filter and a cell filtering device, and relates to the field of medical treatment. Wherein the cell filter comprises a filter pipe, a mounting seat, a filter membrane and a pressure release valve, the filter pipe is provided with a mounting position and a liquid discharge hole, the mounting seat is arranged in the mounting position and is in sealing connection with the filter pipe, the mounting seat is provided with a filter hole and a pressure release hole, the filter membrane is laid on the filter hole, the pressure release valve is arranged in the pressure release hole, and the filter hole is communicated with the liquid discharge hole. Or the pressure relief hole is communicated with the liquid discharge hole; the filtering function of the cell filter is achieved by communicating the filtering hole with the liquid discharging hole, when the filtering membrane is not smooth in filtering or blocked, the effect of discharging blocked liquid is achieved by communicating the pressure relief hole with the liquid discharging hole, the situation of not smooth filtering or blocking is avoided, and the success rate of CTCs detection is increased.

The invention belongs to the technical field of bacterial culture, and discloses a high-throughput automatic monoclonal screening and picking workstation, which comprises a protective box for protection and isolation, and a workbench is arranged in the protective box; the plate stack module comprises a rotating plate stack arranged at the bottom end of the workbench, and a plurality of columns of culture dishes are arranged on the rotating plate stack in a lifting manner; the transfer module is arranged on the workbench and used for transferring culture dishes; the pipetting module comprises a pipetting assembly and a material storage assembly, and the pipetting assembly is used for transferring strains in the culture dish into the material storage assembly; the identification module is used for identifying and positioning the strains in the culture dish and then controlling the pipetting assembly to accurately transfer the strains. The device is simple in structure and flexible and convenient to use, high-throughput screening and picking of strains can be fully automatically carried out in a clean closed environment, manual intervention is not needed, the screening efficiency is high, strain pollution caused by manual intervention is avoided, the screening precision is high, the screening cost is reduced, and experiments are greatly facilitated.

A disposable kit for a bioprocessing system includes a tray having a spine having a first window and a second window, at least one bioprocessing vessel received within the tray, a pinch valve manifold removably received by the spine within the first window, a first tubing segment removably received by the spine within the second window, and a second tubing segment removably received by the spine within the second window.

The invention discloses a bacteriophage reagent production filtering device facilitating impurity cleaning, and relates to the technical field of filtering devices.The bacteriophage reagent production filtering device comprises an impurity cleaning mechanism and a collecting frame installed at the bottom of the impurity cleaning mechanism, a mixing mechanism is arranged at the top of the impurity cleaning mechanism, and a feeding pipe is arranged at the top of the mixing mechanism; the impurity cleaning mechanism comprises a filter box, a centrifugal motor is fixedly installed on one side of the middle of the filter box, the output end of the centrifugal motor is fixedly connected with a rotating block, a first rotating support is fixedly installed on one side of the rotating block, and rotating discs are symmetrically installed at the two ends of the first rotating support; a plurality of connecting sliding grooves are formed in the rotating discs, a plurality of first filter screen plates are connected between the two rotating discs in a sliding mode, full filtration of a phage reagent can be achieved by arranging the multiple sets of first filter screen plates, and impurities subjected to preliminary filtration can be filtered for the second time through the centrifugal effect of the multiple sets of first filter screen plates.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

A bioreactor systems and processes for the production of biomass are provided. The systems comprise: at least one bioreactor unit which comprises at least one liquid-containable compartment. The liquid- containable compartment comprises, (i) a first wall, wherein the first wall comprises a composite membrane that is permissible to transfer of gases therethrough, wherein the composite membrane comprises at least one barrier layer and at least one reinforcement layer; and (ii) a second wall, wherein the second wall comprises material that is optically transmissible to visible light and that is substantially less gas permeable than the first wall, wherein the first and second walls cooperate to define the liquid-containable compartment within the bioreactor unit.