4586results about "Apparatus sterilization" patented technology

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

Described herein are systems and methods for automated cell transduction within a cell processing system. A system for cell processing may include a cell processing cartridge having a transduction system. The transduction system may include a fluidic manifold, one or more modules for performing a cell processing protocol, and a tube having a surface area to volume ration of between about 1,260 mm2 / mL and about 5,080 mm2 / mL. A method for cell processing may first include flowing cells through a tube of a flow cell of a cell processing cartridge for a first time period to achieve a transduction efficiency of at least 50%. Second, the method may include expanding the cells within a bioreactor module of the cell processing cartridge for a second time period.

The present disclosure provides systems and methods for processing cells. The systems of the present disclosure may include a compartment, a three-dimensional (3D) matrix, a fluid flow path, an analysis unit, a preservation unit, or any combination thereof. The methods of the present disclosure may process cells via, for example, expansion, culturing, gene delivery, activation, or any combination thereof. The systems and methods described herein may be useful for producing cell products including, for example, cell therapy products.

Provided is a novel cooling tower for xylanase preparation, comprising a water tank platform, a tower body, and support columns, wherein a circular ring block is rotatably connected to the inner wall at the bottom of the water tank platform, an annular cavity is formed along the inner wall of the circular ring block, a plurality of straight rods are evenly distributed in, penetrate through, and are slidably connected to the inner wall of the annular cavity, a scraper is fixedly connected to one end of each straight rod, and the scrapers slidably abut against the inner wall at the bottom of the water tank platform; and a power mechanism for driving the scrapers to clean impurities inside the water tank platform is arranged inside the annular cavity. An annular rack, a plurality of gears, the straight rods, and a scraper structure are provided, the output end of a motor drives rotating shafts fixedly connected thereto to rotate by a certain angle, and by means of the gears, the annular rack, and a toothed belt structure, the rotating shafts drive the straight rods and the scrapers to slide a certain distance, so that the scrapers scrape some impurities remaining on the inner wall at the bottom of the water tank platform, until these impurities are scraped and accumulated in a circular platform hole, and then discharged to the outside for collection.

A disposable kit for a bioprocessing system includes a tray having a spine having a first window and a second window, at least one bioprocessing vessel received within the tray, a pinch valve manifold removably received by the spine within the first window, a first tubing segment removably received by the spine within the second window, and a second tubing segment removably received by the spine within the second window.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

A device for modelling physiological and pathophysiological states of the brain is provided. The device comprises a hydrogel with micropattern channels having a length, width and depth. The micropattern channels comprise cells selected from the group consisting of neurons, astrocytes, microglia, oligodendrocytes, neuronal organoids, cancer cells, cancer spheroids, brain tumoral cells and combinations thereof.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture tank device which comprises a culture tank, a concave observation area is arranged in the center of the top of the culture tank, a central culture area surrounding the observation area is arranged in the culture tank, an oil path temperature control area wraps the culture tank, and a double-helix heat conduction oil path is integrated in the oil path temperature control area. Through the cooperation of the uniform distributor and the distributed simulated liquid conveying unit, the gas-liquid proportion and fluid distribution can be accurately regulated and controlled, the physical and chemical conditions in the deep sea or extreme environment are simulated, the constant-temperature precision of + / -0.005 DEG C is realized through the double-helix heat conduction oil way, the low-temperature environment is stably maintained, and the real thermodynamic conditions of deep-sea sediments are simulated; the device meets the sensitive requirements of different microorganisms on temperature, and has the characteristics of high practicability and capability of simulating a dynamic fluid environment of deep sea.

The invention discloses an efficient denitrification method and system for landfill leachate, and the method comprises the following steps: diluting waste molasses, and then adding a compound enzyme preparation containing cellulase, pectinase and alpha-amylase for enzymolysis pretreatment to obtain a pretreatment solution; inoculating the pretreatment liquid with a compound flora containing clostridium butyricum and succinic acid-producing anaerobic spirillum, carrying out anaerobic fermentation, and separating to obtain a liquid carbon source containing acetic acid and propionic acid and fermentation residues; mixing the fermentation residues with waste sugar residues, and performing pyrolysis to prepare biochar; and synchronously adding the liquid carbon source and the biochar into a denitrification reactor, and carrying out denitrification on the landfill leachate. The total nitrogen removal load of the landfill leachate denitrification system is increased by 60% compared with that of a single carbon source process, and the sugar manufacturing solid waste recycling rate gt is achieved; 95%. According to the method disclosed by the invention, the dual goals of efficient denitrification and solid waste recycling are achieved through cooperative treatment of the waste molasses and the waste sugar residues in the sugar manufacturing process.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A fully automated chemiluminescence immunoassay analyzer, including a sample and reagent receiving device for receiving a sample and a reagent, a dispensing device for aspirating and discharging the sample and the reagent, a mixing device for mixing the sample and the reagent in a reaction vessel, an incubation and luminescence detection device for incubation and luminescence detection, a magnetic separation cleaning device for separation cleaning an analyte and impurities in the reaction vessel, a reaction vessel grasping device for transferring the reaction vessel, and a liquid path device.

The automatic feed adjusting type vacuum rotary drum comprises symmetrically-arranged supporting seats, a material storage groove, a rotary drum structure and a spraying structure are arranged between the symmetrically-arranged supporting seats, a vacuum pump and a transmission structure are arranged on the two sides of the rotary drum structure respectively, and an adjusting material scraping structure, a material supplying and pressing structure and a material guiding structure are arranged on the material storage groove. And a plurality of groups of camera structures are arranged on the wall body close to one side of the adjusting scraping structure. By the adoption of the structure, the whole-process automatic monitoring and adjusting of the material treatment process are achieved, the efficiency and accuracy of screening, cleaning and finish machining are remarkably improved, it is ensured that machining parameters are adjusted in time, material accumulation or loss is avoided, and therefore the continuity and stability of the machining process are kept; due to the design of a multi-layer filtering structure, impurities are removed in a layered mode according to material characteristics, especially when high-humidity materials are treated, the screen mesh can be effectively prevented from being blocked, and the high quality of the treated materials is guaranteed.

An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

The invention discloses a portable nucleic acid detection micro-fluidic chip, a nucleic acid detection device and a nucleic acid detection method, and belongs to the field of micro-fluidic chips, a detection module comprises a quantitative chamber, a micro-channel, a reaction chamber, a first connecting channel and a color developing module; the reaction chamber comprises an RPA reaction chamber in which an RPA amplification reagent is pre-embedded, a porous filter membrane and a CRISPR reaction chamber in which a CRISPR detection component is pre-embedded, and the RPA reaction chamber, the porous filter membrane and the CRISPR reaction chamber are sequentially positioned at the same position of the three adjacent chip layers; the aperture of the porous filter membrane is larger than the particle size of an RPA amplification product and smaller than the particle size of a core enzyme in a CRISPR detection component; two ends of the micro-channel are respectively communicated with the quantitative chamber and the RPA reaction chamber; two ends of the first connecting channel are respectively communicated with the CRISPR reaction chamber and the color developing module; the flow resistance of the first connecting channel is smaller than that of the micro-channel. According to the invention, the sequential reaction of RPA amplification and CRISPR cleavage can be completed without depending on artificial transfer of amplification products.

The invention discloses a micro-fluidic chip, extraction equipment and an exosome extraction method, which can be used for an enzymatic detection device. Wherein the micro-fluidic chip comprises a chip base body with a rotation center, the chip base body is provided with a sample introduction unit, a reagent unit, a filtering unit, a capturing unit, a waste liquid bin, a collecting unit and a switching structure, and the filtering unit is arranged on the downstream of the sample introduction unit in the centrifugal direction, communicates with the sample introduction unit and is used for filtering impurities in a sample; the capturing unit is arranged on the downstream of the filtering unit in the centrifugal direction and communicated with the filtering unit, the capturing unit is communicated with the reagent unit and located on the downstream of the reagent unit, and the capturing unit is used for capturing or releasing the extracted target object; the waste liquid bin and the collecting unit are arranged on the downstream of the capturing unit in the centrifugal direction, and the waste liquid bin communicates with the capturing unit through a communicating structure; the switching structure is arranged in the communication structure, the switching structure can selectively block the communication structure, and the switching structure can enable the capturing unit to be communicated with the collecting unit.

The invention discloses a soil microbial fermentation device and a fermentation method, and relates to the field of microbial fermentation. A soil microorganism fermentation device comprises a fermentation tank and further comprises a cover plate detachably connected to the upper end of the fermentation tank; the material conveying barrel is positioned in the fermentation tank and is rotationally connected to the cover plate; the rotating sleeve is connected to the material conveying barrel in a sleeving mode, and a shifting rod is fixedly connected to the rotating sleeve; the motor drives the bevel gear for transmission, the linkage conveying barrel and the rotating shaft to rotate, and the electric telescopic rod is matched to drive the rotating sleeve to reciprocate, so that the three-dimensional stirring of the driving lever and the circulating conveying of the spiral blade are realized, a multi-dimensional stirring mode is formed, the material mixing uniformity is improved, the full contact of microorganisms and nutrient substances is promoted, and the fermentation efficiency and quality are improved; the elastic deflector rod and the counterweight ball can adaptively adjust the stirring range along with the rotating speed, eliminate stirring dead angles, accelerate the fermentation process, shorten the period, reduce the cost, and further optimize the fermentation effect and the product quality.

The invention belongs to the technical field of biological sewage treatment, and relates to a bacteria-algae particle preparation method and system for promoting bacteria-algae system granulation based on pressure response. The preparation method comprises the following steps: co-culturing the mixed microalgae cultured to a logarithmic phase and aerobic activated sludge in a photobioreactor, in the culture process, jointly regulating and controlling aeration, a carbon source and inlet and outlet water to promote granulation of a bacteria-algae system according to the principle of'pressure-induced stress response ', and controlling dissolved oxygen levels in different periods according to the principle of'unbalanced air supply' during aeration regulation and control; the carbon source regulation follows the principle of unbalanced nutrition to control the COD concentration in different time periods; the water inlet and outlet regulation follows the principle of reducing the settling time, and the water change settling time is gradually shortened in the culture period. The bacteria-algae granular sludge which is good in settling performance, high in dirt-removing power and compact in structure is prepared, the prepared bacteria-algae granular sludge is used for treating sewage and wastewater, pollution of carbon, nitrogen, phosphorus and the like can be efficiently solved, the effluent quality is effectively improved, and carbon emission reduction in the sewage treatment field is promoted.

The invention relates to the technical field of biological medicine preparation equipment, and discloses an intelligent CAR-T cell culture system, which comprises an intelligent temperature control and cleaning box body, a double sterilization system and a full-automatic preparation process, and the intelligent temperature control and cleaning box body adopts temperature and humidity control and cleanliness control. The double sterilization system comprises hydrogen peroxide fumigation and ultraviolet circulation disinfection, and the full-automatic preparation process comprises a precise pipeline, a modular reaction unit and an automatic control platform. According to the intelligent CAR-T cell culture system, full-process automatic culture of full-automatic CAR-T cells is realized in a C-level environment, a high-requirement clean environment is not needed, the cost can be greatly reduced, the economic benefit is remarkable, a full-closed pipeline system better meets the requirements of laws and regulations, and the system has greater advantages in the supervision level; the full-automatic operation reduces the intervention of personnel, avoids the potential pollution risk in the process, the full-process automatic cultivation can greatly save the cost, and the cost is saved by 40% compared with the traditional process through comprehensive calculation.

A bionic organ device includes an organ chip and a power module. The organ chip includes a first body, a second body, a porous film and a piezoelectric element. The porous film is disposed between the first body and the second body and forms a flow channel system with the first body and the second body. The flow channel system includes a first passage and a second passage. The first passage is located between the first body and the porous film, and the second passage is located between the second body and the porous film. The piezoelectric element is disposed on one side of the porous film and is connected to the porous film and is electrically connected to the power module. The power module drives the piezoelectric element to deform, and the deformation of the at least one piezoelectric element drives the size of the porous film to change.

The invention relates to the technical field of strain extraction, in particular to a microbial fermentation strain extraction and detection device for a fermented beverage. Comprising a tank body, and a tank cover is fixedly mounted at the upper end of the tank body; a feeding hole communicated with the tank body is formed in the upper end of the tank cover; by arranging the partition plate, the interior of the tank body is divided into two independent cavities by the partition plate, so that a sampler firstly enters the cavity above the partition plate, and infectious microbes in external air are sterilized when entering the cavity above the partition plate through the sampling port; then the sterilized sampler samples the strains cultured in the chamber below the partition plate through the U-shaped pipe, so that infectious microbes suspended in the air above are prevented from directly falling into the chamber below the partition plate, and the risk that the strains in the chamber below are contaminated is reduced; the safety of strain extraction is guaranteed, the extracted strains are prevented from being polluted by infectious microbes in the air, the fluctuation range of the detection result is reduced, and the detection data are more accurate.

The invention belongs to the technical field of exosome preparation, and discloses an efficient preparation device for exosome production, the efficient preparation device comprises an assembly plate, a cultivation cylinder, a filter part and a peristaltic conveying pump, a temporary storage cylinder, an enrichment collection assembly, a tangential ultrafiltration mechanism and a return pipe are arranged above the assembly plate; and the temporary storage cylinder, the enriching and collecting assembly, the tangential ultrafiltration mechanism and the return pipe are communicated in sequence. Through the elastic reset effect of the first spring, shaking of the ultrafiltration membrane installed in the assembly ring is achieved, it is guaranteed that the ultrafiltration membrane is in a dynamic state in the exosome ultrafiltration interception process, it is avoided that a large number of exosomes are accumulated in the local area of the front face of the ultrafiltration membrane, hydraulic pressure is prevented from acting on the exosomes accumulated in the ultrafiltration membrane fixing area, and the effect of the hydraulic pressure on the exosomes is avoided. According to the present invention, with the dynamic ultrafiltration process, the exosome accumulation in the fixed area is reduced, the hydraulic force and the exosome filtration interception acting force are dispersed at all positions of the ultrafiltration membrane, the stress uniformity in the ultrafiltration process is provided, and the service life is prolonged.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

The invention relates to the technical field of biological fermentation devices, and particularly discloses an efficient fermentation device for beef cattle feed processing, the efficient fermentation device comprises a fermentation tank, the upper end of the fermentation tank is fixedly connected with a feeding pipe, the upper end of the fermentation tank is provided with a pressure stabilizing assembly for exhausting, and the inner surface of the fermentation tank is fixedly connected with a guide seat; the pressure stabilizing assembly comprises a fixing cover fixedly connected with the outer surface of the upper end of the fermentation tank, notches are symmetrically formed in the outer surface of the fixing cover, and a sealing plate is slidably connected to the inner surface of the fixing cover. By arranging the pressure stabilizing assembly, the gas in the fermentation tank can be discharged in time according to the pressure change of the gas in the fermentation tank, so that the pressure in the fermentation tank can be maintained in a proper range, the risk that the fermentation tank is burst by pressure can be reduced, and meanwhile, the stability of the fermentation tank in the working process can be improved; furthermore, the fermentation quality of the cattle feed can be ensured, and the workload of manual exhaust of workers can be reduced.

The invention discloses a method for separating, sorting and concentrating rare cells. The method comprises the following steps: pretreating a to-be-separated sample, adding the pretreated sample into a sample accommodating assembly in the cell separation, sorting and concentration device, and controlling the sample accommodating assembly to separate, sort and enrich the to-be-separated sample through a filtering and sorting mechanism to obtain rare cells. The specific cell separating, sorting and concentrating device is designed, and the method for separating, sorting and concentrating the rare cells is developed based on the device, so that the recovery rate and concentration rate of the rare cells are improved, the operation is simplified, the smear operation process is further developed, the separation, sorting, enrichment and smear processes of the cells can be realized, and the production efficiency is improved. The cell loss is prevented, the smear quality is improved, and the smear time is shortened.

A device and method for assembling objects by generating an axial acoustic radiation force in order to position the objects on pressure nodes and / or antinodes and generating a transverse acoustic radiation force in order to move the thus positioned objects on a porous membrane contact structure.