3614results about "Apparatus sterilization" patented technology

The invention relates to the technical field of microorganism culture, and discloses a microorganism culture tank device which comprises a culture tank, a concave observation area is arranged in the center of the top of the culture tank, a central culture area surrounding the observation area is arranged in the culture tank, an oil path temperature control area wraps the culture tank, and a double-helix heat conduction oil path is integrated in the oil path temperature control area. Through the cooperation of the uniform distributor and the distributed simulated liquid conveying unit, the gas-liquid proportion and fluid distribution can be accurately regulated and controlled, the physical and chemical conditions in the deep sea or extreme environment are simulated, the constant-temperature precision of + / -0.005 DEG C is realized through the double-helix heat conduction oil way, the low-temperature environment is stably maintained, and the real thermodynamic conditions of deep-sea sediments are simulated; the device meets the sensitive requirements of different microorganisms on temperature, and has the characteristics of high practicability and capability of simulating a dynamic fluid environment of deep sea.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

The invention discloses a portable nucleic acid detection micro-fluidic chip, a nucleic acid detection device and a nucleic acid detection method, and belongs to the field of micro-fluidic chips, a detection module comprises a quantitative chamber, a micro-channel, a reaction chamber, a first connecting channel and a color developing module; the reaction chamber comprises an RPA reaction chamber in which an RPA amplification reagent is pre-embedded, a porous filter membrane and a CRISPR reaction chamber in which a CRISPR detection component is pre-embedded, and the RPA reaction chamber, the porous filter membrane and the CRISPR reaction chamber are sequentially positioned at the same position of the three adjacent chip layers; the aperture of the porous filter membrane is larger than the particle size of an RPA amplification product and smaller than the particle size of a core enzyme in a CRISPR detection component; two ends of the micro-channel are respectively communicated with the quantitative chamber and the RPA reaction chamber; two ends of the first connecting channel are respectively communicated with the CRISPR reaction chamber and the color developing module; the flow resistance of the first connecting channel is smaller than that of the micro-channel. According to the invention, the sequential reaction of RPA amplification and CRISPR cleavage can be completed without depending on artificial transfer of amplification products.

The invention discloses a micro-fluidic chip, extraction equipment and an exosome extraction method, which can be used for an enzymatic detection device. Wherein the micro-fluidic chip comprises a chip base body with a rotation center, the chip base body is provided with a sample introduction unit, a reagent unit, a filtering unit, a capturing unit, a waste liquid bin, a collecting unit and a switching structure, and the filtering unit is arranged on the downstream of the sample introduction unit in the centrifugal direction, communicates with the sample introduction unit and is used for filtering impurities in a sample; the capturing unit is arranged on the downstream of the filtering unit in the centrifugal direction and communicated with the filtering unit, the capturing unit is communicated with the reagent unit and located on the downstream of the reagent unit, and the capturing unit is used for capturing or releasing the extracted target object; the waste liquid bin and the collecting unit are arranged on the downstream of the capturing unit in the centrifugal direction, and the waste liquid bin communicates with the capturing unit through a communicating structure; the switching structure is arranged in the communication structure, the switching structure can selectively block the communication structure, and the switching structure can enable the capturing unit to be communicated with the collecting unit.

The invention discloses a soil microbial fermentation device and a fermentation method, and relates to the field of microbial fermentation. A soil microorganism fermentation device comprises a fermentation tank and further comprises a cover plate detachably connected to the upper end of the fermentation tank; the material conveying barrel is positioned in the fermentation tank and is rotationally connected to the cover plate; the rotating sleeve is connected to the material conveying barrel in a sleeving mode, and a shifting rod is fixedly connected to the rotating sleeve; the motor drives the bevel gear for transmission, the linkage conveying barrel and the rotating shaft to rotate, and the electric telescopic rod is matched to drive the rotating sleeve to reciprocate, so that the three-dimensional stirring of the driving lever and the circulating conveying of the spiral blade are realized, a multi-dimensional stirring mode is formed, the material mixing uniformity is improved, the full contact of microorganisms and nutrient substances is promoted, and the fermentation efficiency and quality are improved; the elastic deflector rod and the counterweight ball can adaptively adjust the stirring range along with the rotating speed, eliminate stirring dead angles, accelerate the fermentation process, shorten the period, reduce the cost, and further optimize the fermentation effect and the product quality.

The present disclosure relates to systems, devices, and methods for automated fluid transfer. In an embodiment, the present disclosure relates to a system for fluid transfer between a fluid device and a cartridge for cell processing. The system comprises a first portion comprising an instrument head coupled to a gantry, the instrument head comprising a pneumatic actuator, a peristaltic pump, and a gripping feature for receiving the fluid device. The peristaltic pump may be configured to engage compressible fluidic tubing of a fluid pump module of the fluid device to transfer the fluid between the fluid device and the cartridge. The system further comprises a second portion comprising a docking station for receiving the cartridge. In some variations, movement of the pneumatic actuator relative to the fluid pump module causes occlusion of the compressible fluidic tubing of the fluid pump module by the peristaltic pump.

A device and method for assembling objects by generating an axial acoustic radiation force in order to position the objects on pressure nodes and / or antinodes and generating a transverse acoustic radiation force in order to move the thus positioned objects on a porous membrane contact structure.

The present invention relates to an integrated system for automating cell culture processes. The system combines artificial intelligence (AI) with a robotic apparatus to execute tasks typically performed manually in cell culture laboratories. The AI module employs machine learning algorithms trained on extensive datasets, enabling it to make informed decisions regarding cell culture conditions and protocols for a variety of cell types. An accompanying robotic system performs liquid handling tasks such as media changes and cell passaging with precision. The system is compatible with multiple types of cell culture vessels, facilitating bulk processing. Additionally, an enclosed sterile environment is maintained to prevent contamination. A user interface allows for the customization of protocols and remote monitoring, enhancing operational efficiency. This invention streamlines cell culture workflows, reduces manual labor, and increases the reproducibility and scalability of cell culture.

The invention discloses an air microorganism aerosol alarm sampling system and method and an unmanned aerial vehicle system. A main controller receives a current position positioned by a GPS positioning module, and when the current position is in a to-be-detected area, an air pump and an alarm sampling optical module are started to pump air into the alarm sampling optical module; the alarm sampling optical module samples microbial particles in the air to form a fluorescence signal, the main controller analyzes the aerosol concentration according to the fluorescence signal and identifies whether the aerosol is a biological aerosol, when the aerosol concentration exceeds a set threshold value or the aerosol is identified as the biological aerosol, the alarm is controlled, and meanwhile, the sampling module is controlled to be started; the main controller controls the liquid supplementing peristaltic pump to start, the sampling liquid in the sampling liquid bottle is pumped into the sampler, then the motor driver is controlled to drive the fan to start to enable air to enter the sampler for sampling, only the sampling peristaltic pump is controlled to start after sampling is completed, and the sample liquid in the sampler is conveyed into the sample bottle through the three-way pipe. The collection of the microbial aerosol particles is completed.

The invention relates to the technical field of egg inoculation, in particular to an egg inoculation machine capable of achieving safe inoculation and an operation method.The egg inoculation machine comprises a conveying unit, an optical detection unit, a mechanical arm assembly and an inoculation assembly; the conveying unit is used for conveying eggs, the position of an air chamber is recognized through the optical detection unit, an injection point is determined, the inoculation assembly is installed on the mechanical arm assembly, and the mechanical arm assembly drives the inoculation assembly to conduct inoculation along the injection point; according to the egg inoculation machine, after punching is completed, the injection needle tube is directly inserted into an egg along a punched hole for safe inoculation, the requirement for visual positioning of the hole can be reduced, a mechanical arm assembly low in precision can be used, and the device cost is reduced.

This disclosure relates to large-scale bioreactor systems comprising a vessel comprising internal reaction chamber having a volumetric and cell-sustaining capacity significantly above that of currently available bioreactor systems.

A biological indicator includes: a BI housing; a germinant container inside the BI housing and housing a germinant composition; a germinant releaser configured to release the germinant composition from the germinant container; a germinant releaser support supporting the germinant releaser and configured to bring the germinant releaser into contact with the germinant container upon application of a force to the germinant releaser support or the germinant container; a first spore carrier inside the BI housing, the first spore carrier having a plurality of spores deposited at a first surface thereof; and an imaging window at a first surface of the BI housing. A BI reader is configured to detect and quantify the presence of live spores in the BI, and includes an excitation source, a camera for capturing images of the spores over time, and a processor for analyzing the images to determine the presence of live spores.

Disclosed herein are systems and devices for wound therapy and related methods of use. The systems and devices for wound therapy and related methods of use disclosed herein may comprise one or more cartridges for preparation of a regenerative epidermal suspension. One or more cartridges for preparation of a regenerative epidermal suspension may comprise a cover with a raised processing opening, a cup that can be received within the raised processing opening and has a screen, and a well plate that is positioned under the cover, has a well, and rotates relative to the cover such that the well aligns with the raised processing opening. One or more cartridges for preparation of a regenerative epidermal suspension comprises a cover with an opening that can receive a tissue sample.

The invention discloses a portable field screening device for environmental remediation microbial strains, and relates to the field of environmental remediation. The invention relates to a portable field screening device for environmental remediation microbial strains, which comprises a tank body which is a high-strength and light-weight aluminum alloy tank, and further comprises a filter cartridge arranged in the tank body, a filter element arranged in the tank body, and a filter element arranged in the tank body, the driving motor is fixedly connected to the upper end of the tank body; the driving shaft is fixedly connected to the output end of the driving motor; solid-liquid separation of the soil turbid liquid is efficiently completed through a centrifugal and ultrafiltration graded filtration mode, compared with a traditional method, the device has the advantages of being high in portability, high in separation efficiency and good in effect, impurities can be accurately intercepted, microbial losses are reduced, microbial strains with specific functions are rapidly screened for an environment restoration site, a high-quality bacterium-containing liquid sample is provided, and the method is suitable for popularization and application. The efficiency and accuracy of screening of microbial strains in environment restoration work are improved, and efficient development of the environment restoration work is promoted.

The application provides a multi-gas control device, a use method and an application, and relates to the technical field of biological gas fermentation. The device comprises a gas supply module, the gas supply module comprises multiple groups of gas sources and connecting pipelines, each gas source is connected to an air inlet pipeline through a respective connecting pipeline, a secondary pressure reducing valve, a T-shaped filter, a mass flow meter, a stop valve, a regulating valve and a check valve are sequentially arranged on the connecting pipeline, the air inlet pipeline is connected to a liquid circulation pipeline, gas is discharged from a spraying mechanism connected to the liquid circulation pipeline, the spraying mechanism is arranged in a fermentation tank, the spraying mechanism is connected to a circulating pump through the liquid circulation pipeline, the circulating pump is connected to the bottom of the fermentation tank through a pipeline, the top of the fermentation tank is connected to a detection pipeline, and a precision filter, a vacuum pump, a drying device, a pre-analyzer and a gas analyzer are sequentially arranged on the detection pipeline. The application realizes efficient absorption and utilization of multiple gases, and simultaneously obtains high yield and high quality single-cell protein.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

Disclosed is a method and a device for biological production of sulfuric acid. The disclosure allows the use of high concentrations of sulfur as a feed for microbiological oxidation, resulting in high conversion of sulfur to sulfuric acid and, consequently, high sulfuric acid yields, which are obtained in an environmentally friendly way.

The invention provides a fruit juice beverage acetic acid fermentation device, and relates to the technical field of fermentation devices.The fruit juice beverage acetic acid fermentation device comprises a fermentation tank, a heat preservation box is arranged on the peripheral side of the fermentation tank, a fermentation cavity and a storage cavity are formed in the fermentation tank, a driving structure is arranged on the upper side of the fermentation tank, a first stirring structure is arranged in the storage cavity, and a second stirring structure is arranged in the fermentation cavity; a connecting and discharging structure is arranged between the first stirring structure and the second stirring structure, a filtering structure is arranged in the storage cavity, and the filtering structure corresponds to the first stirring structure. According to the invention, the first stirring structure is mounted in the storage cavity, the second stirring structure is mounted in the fermentation cavity, the discharging structure is mounted and connected between the first stirring structure and the second stirring structure, and the spraying structure is mounted on the second stirring structure, so that fruit juice can be stirred through the first stirring structure and the second stirring structure; vortex is prevented through the baffle, the stirring effect is guaranteed, and therefore it can be guaranteed that fruit juice and acetic bacteria can be fully mixed.

The utility model relates to the technical field of mineralizing bacteria equipment, in particular to an oil reservoir CO2 mineralizing bacteria activity continuous monitoring device which comprises an incubator filled with a culture medium for culturing mineralizing bacteria. The air outlet end of the air supply assembly is located on the lower side in the incubator; the carbon dioxide concentration monitoring assembly is mounted at the upper end part of the incubator; the strain concentration monitoring assembly is mounted on the side wall of the incubator and is communicated with the incubator; the control assembly is electrically connected with the air supply assembly, the carbon dioxide concentration monitoring assembly and the strain concentration monitoring assembly. The activity of mineralized bacteria can be rapidly and continuously monitored, and the monitoring efficiency is improved.

The invention relates to a fermentation device and a preparation method of medicinal and edible food and medicated diet formula food, and belongs to the technical field of food fermention.The fermentation device comprises a supporting seat, a main fermentation tank is arranged at the top of the supporting seat, an enzymolysis tank is fixedly connected to the top of the main fermentation tank, and a pretreatment tank is fixedly connected to the top of the enzymolysis tank. By arranging connecting fan blades, when raw materials are pre-crushed through a crushing cutter set, in order to improve the multifunctional conversion and utilization effect of mechanical energy generated by rotation of the crushing cutter set and prevent the raw materials from being adsorbed on the inner wall of a pretreatment tank to affect the full crushing effect of the raw materials, rotation of a first bevel gear set can improve the crushing effect of the raw materials; through rotation of blowing fan blades, gas is conveyed into an annular pipe through a gas conveying pipe and conveyed into a connecting nozzle through a fixing pipe, meanwhile, gas is input into the pretreatment tank under the condition that gas spraying holes are formed, and the raising and smashing effect of raw materials in the pretreatment tank is improved.

The invention discloses a modified microfiltration membrane for enriching biological particles and a preparation method and application thereof, and belongs to the technical field of biological separation.The preparation method of the modified microfiltration membrane comprises the following steps that a mixed cellulose ester microfiltration membrane is provided and subjected to alkali liquor activating treatment; placing the activated membrane in a weakly alkaline buffer solution for balancing; the preparation method comprises the following steps: in a weakly alkaline buffer solution, firstly contacting the surface of the membrane with a tannic acid solution to carry out a first reaction, and then directly adding a polyethyleneimine solution to carry out a second reaction under the condition of not replacing a system, so as to form a phenol-amine cross-linked network on the surface of the membrane in situ; sodium cyanoborohydride is continuously added for reduction stabilization treatment, and the modified microporous filter membrane is obtained. The modified microporous filter membrane prepared by modifying the microporous filter membrane with tannic acid and polyethyleneimine can realize one-step synchronous enrichment of all negatively charged targets, and the problems of target loss, efficiency loss and the like caused by a multi-step process are avoided from the source.

The invention relates to the technical field of fishery breeding tail water treatment, and discloses circulating fishery tail water purification equipment based on photosynthetic microorganisms, the circulating fishery tail water purification equipment comprises a photosynthetic reaction treatment box and a biomass collection box, an LED light source module and a lamp tube surface cleaning assembly are arranged in the photosynthetic reaction treatment box, and a waste gas collection and circulation assembly is arranged at the top of the photosynthetic reaction treatment box; an inclined plate type grating plate and a grating plate impurity scraping assembly are arranged in the biomass collecting box, and an inner circulation and outer discharge assembly of photosynthetic microorganisms is arranged at the bottom of the biomass collecting box. The lamp tube surface cleaning assembly conducts multi-dimensional cleaning on the surface of a light source through linear and rotary composite motion, and the illumination efficiency is guaranteed; carbon-rich waste gas is recycled through the waste gas collecting and circulating assembly, dehumidified and then re-injected into the water body, and carbon source circulation is achieved; the grating plate impurity scraping component and the internal circulation component are used for regularly cleaning the blockage of the grating and reflowing high-activity strains, so that the stable operation of the system is maintained, and the purification efficiency is improved.

The utility model provides a homogenizing device for food inspection. The homogenizing device for food inspection comprises a device body, the same opening is formed in the two adjacent sides of the device body, splash-proof observation glass is arranged on one side of the device body, a hinge is rotationally installed between the splash-proof observation glass and the device body, a base is arranged in the device body, and the splash-proof observation glass is connected with the base. The device comprises a device body, the top of the device body is fixedly provided with a base, the top of the base is fixedly provided with a homogenate barrel, the homogenate barrel and the base are internally and fixedly provided with a filter plate and a collecting barrel respectively, the top of the device body is fixedly provided with a hydraulic cylinder, and the device body is internally provided with a lifting seat. The homogenizing device for food inspection has the advantages that the use is convenient, the inner wall of the homogenizing cylinder can be cleaned, and the operation is simple.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The invention relates to the technical field of giant salamander peptide separation and purification, in particular to a giant salamander peptide extraction and separation and purification method and system.The method comprises the steps that healthy giant salamanders and giant salamander processing equipment are determined, giant salamander processing by-products are obtained, the giant salamander processing by-products are pretreated, to-be-processed by-products are obtained, the to-be-processed by-products are degreased and deodorized, and the giant salamander peptide is obtained. Preparing giant salamander protein dry powder, preparing giant salamander crude peptide liquid, obtaining a target peptide fragment, finely separating the target peptide fragment to obtain target active peptide distillate, freeze-drying the target active peptide distillate to obtain high-purity giant salamander peptide powder, and storing the molecular weight, amino acid sequence and activity verification parameters of the peptide fragment. And extracting, separating and purifying the giant salamander peptide based on the optimized storage database and the giant salamander grease. The extraction efficiency and the separation and purification efficiency of the giant salamander peptide can be improved.

The invention relates to the technical field of medical equipment, in particular to a carbon dioxide gas supply device for cell culture, which comprises a shell, the shell is provided with a mixing device, gas enters the mixing device for mixing through a gas inlet pipe, and the mixed gas enters a heating module through a gas outlet pipe. The heating module heats the mixed gas and then enters the cell culture box; spiral assemblies are symmetrically arranged in the mixing device and comprise fixed spiral bodies and movable spiral bodies which are distributed in a staggered mode, the fixed spiral bodies are fixedly connected with the mixing device, and the movable spiral bodies are rotationally connected with the fixed spiral bodies; the compression assembly is arranged below the spiral assembly and is in transmission connection with the movable spiral body, the mixing degree and the compression degree of the compression assembly are finely regulated and controlled according to cell requirements, flexible switching between static mixing and dynamic mixing is realized, and the mixing uniformity is guaranteed so as to adapt to the gas supply requirements of different cells.