47 results about "Cellular respiration" patented technology

The invention relates to the technical field of agricultural product preservation, and particularly discloses an ethylene-gas-based accurate mango ripening method which specifically comprises the following steps: cleaning and sorting, ultrasonic degumming, bacteriostasis and preservation, packaging, ripening treatment and delivery from a warehouse. Wherein the cleanliness of the surfaces of the mangoes is improved by adopting a degumming agent in cooperation with ultrasonic waves, and gummosis is prevented from damaging the mangoes while the gummosis is more sensitive to ethylene gas for subsequent ripening; the plasma citric acid solution is used for treating mango to activate cells in vivo, so that the respiration in the mango ripening process is enhanced, and the ethylene peak is advanced to promote the ripening process; the terahertz electromagnetic wave technology can enable cells to form an ordered state, so that the consistency of cells in different parts of the fruits in response to ethylene ripening acceleration is improved, and uniform ripening of mangoes is promoted; according to the method, gas ethylene is used for ripening treatment, the food safety risk of traditional ethephon residues can be reduced, and the ripening commodity rate is increased.

The invention relates to the technical field of sewage treatment, in particular to a sewage treatment device for treating COD and nitro nitrogen and a sewage treatment method.The sewage treatment device comprises a treatment part making contact with sewage, and a carbon material modification layer is arranged on the surface, making contact with the sewage, of the treatment part; sewage treatment microorganisms screened by low-voltage current are inoculated on the carbon material modified layer; the carbon material modified layer comprises a carbon material and a binder; the carbon material comprises a net-shaped or long-strip-shaped carbon material and a granular carbon material. According to the sewage treatment method, COD and NO3 <->-N in sewage are degraded simply and efficiently through the conductive microorganisms, so that the effect of removing pollutants in a water body is achieved, and degradation of the pollutants can be accelerated through the respiration effect of the conductive microorganisms in the sewage treatment method. As oxygen does not need to participate in the degradation process of the conductive microorganisms, the device does not need strict aerobic and anaerobic treatment, and the method is simple and convenient in process and low in cost.

The invention discloses a cell culture device for gene detection, and belongs to the field of gene detection. A cell culture device for gene detection comprises a detection box and a microscope, the detection end of the microscope penetrates into the detection box, the bottom of an inner cavity of the detection box is fixedly connected with a low-speed motor, the top end of the output end of the low-speed motor is fixedly connected with a rotating disc, and three sets of clamping bases are distributed at the top of the rotating disc at equal intervals; a culture dish is inserted into the clamping seat; through rotation of the rotating disc, an upper contact ball and a lower contact ball are in contact extrusion, so that gas in a gas collection box is pushed to repeatedly enter a spring telescopic rod, a clamping seat is pushed to slide back and forth at the top of the rotating disc, and a culture solution in a culture dish and cells are slightly shaken; and nutrient substances are promoted to be uniformly distributed in the culture dish, cells are effectively prevented from settling to the bottom of the culture dish, the respiration of the cells is facilitated, the culture speed of the cells is ensured, and the gene detection efficiency is improved.

The invention belongs to the technical field of carbon capture and sewage treatment, and discloses a method for enhancing microalgae biofilm carbon sequestration and water purification based on a metal organic framework material, which comprises the following steps: adding MOF materials with different types and concentrations into a microalgae biofilm culture system; and carrying out microalgae biological membrane culture and sewage treatment under natural illumination or artificial light supplement. By utilizing the unique property that the MOF material can adsorb CO2, the enrichment of CO2 in a gas phase environment to a microalgae biological membrane system is accelerated; meanwhile, CO2 generated by respiration of microalgae and bacteria in the biological membrane is captured, a microalgae biological membrane growth condition with sufficient inorganic carbon supply is formed, and a micro-pore channel formed in the biological membrane due to addition of the MOF material can also improve the mass transfer condition in the biological membrane. The method is simple and easy and convenient to operate, a large amount of CO2 dissolved in water can be fixed, and pollution reduction and carbon reduction synergistic interaction is achieved while a large amount of microalgae biomass is obtained.

The invention discloses an MT (melatonin) and SNP (single nucleotide polymorphism) compound preservative as well as preparation and application thereof, and relates to the field of compound preservative films. A nitric oxide donor sodium nitroprusside (SNP); tween-80 (Tween-80) is added into auxiliary components to serve as an emulsifying agent, MT and SNP are dissolved in a proper amount of water and evenly stirred, then Tween-80 is added, and the MT and SNP compound preservative is prepared, according to the MT and SNP compound preservative and preparation and application thereof, MT and SNP are natural or quasi-natural components, compared with chemical preservatives, the MT and SNP have smaller potential harm to the environment and the human body, MT and SNP are used in a compound mode, and therefore the MT and SNP compound preservative has the beneficial effects that the MT and the SNP are more environmentally friendly and more environmentally friendly. MT and SNP cooperate with each other to improve the fresh-keeping effect, the antioxidant effect of MT can reduce oxidative damage in the fruits, SNP promotes endogenous NO generation, can regulate signal transduction in the fruits and further inhibits respiration and ethylene synthesis of the fruits, maturing and aging of the fruits are delayed from different angles through cooperation of MT and SNP, and the fresh-keeping effect of the fruits is improved. Meanwhile, the MT and the NO can cooperatively regulate and control signal transduction in the plant body, and the action time of the NO in the fruit body is prolonged.

The present invention relates to an in vitro method for predicting the response to immunotherapy treatment of hepatocellular carcinoma in a subject, comprising the following steps: 1) A step of determining the energy metabolic state of a liver tissue sample from a subject, wherein the sample comprises pathological tissue and optionally non-pathological tissue, 2) A step of detecting the presence or absence of energy conversion resulting from a reduction in mitochondrial respiration in the pathological tissue by comparing it with a liver tissue sample of the subject or a non-pathological tissue from a standard non-pathological liver sample, Here: - If mitochondrial respiration is reduced in pathological tissue, the subject is less likely to respond to immunotherapy treatment, and - If mitochondrial respiration is not reduced in the pathological tissue, the subject is highly likely to respond to immunotherapy treatment.

The utility model discloses integrated buried transfer station wastewater treatment equipment, relates to the field of wastewater treatment, and aims to solve the problems that in the prior art, when water in an anoxic bin is stirred, bubbles can be formed and gathered together to form foams due to the existence of a surfactant or other substances in the water, the respiration of microorganisms is influenced, and the sewage treatment effect is affected. In order to solve the problems of reduction of wastewater treatment efficiency, overflow of wastewater, need of additional treatment and cleaning and increase of treatment cost in the prior art, the utility model provides the following scheme: the device comprises a grating pool, one side of the grating pool is fixedly provided with an adjusting pool bin, an anoxic bin, an aerobic bin, a membrane pool, a clear water bin and an equipment room in sequence, and a basket grating is arranged in the grating pool; and a water passing hole is formed between the grating tank and the adjusting tank bin. According to the device, generated bubbles can be eliminated while the wastewater is stirred, so that the situation that the wastewater overflows, additional treatment and cleaning are needed, and the treatment cost is increased is avoided.

The present invention relates to a non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising an active complex: extracts of Melaleuca alternifolia, extracts of Lavandula hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis.

The invention belongs to the technical field of food processing, and discloses intelligent food fresh-keeping packaging equipment which comprises an equipment shell, a full-process automatic control system, a packaging structure and a conveying device. Through four-stage treatment of the air purification module, three-stage treatment of the water purification module and RO reverse osmosis treatment, multi-dimensional interception, adsorption, filtration and sterilization, the number of air colonies is smaller than or equal to 5 CFU / m < 3 >, the water conductivity is smaller than or equal to 1 microsecond / cm, microorganisms and pollutants are removed from the source, and a safe foundation is built for food processing. Low-temperature plasma and ultrahigh-pressure synergistic sterilization greatly improves the escherichia coli killing rate, greatly shortens the ultrahigh-pressure sterilization time, is efficient, does not damage the food quality, and greatly improves the food hygiene level, and the intelligent controlled atmosphere fresh-keeping system customizes a gas environment for different foods through two-stage gas separation, precise humidity regulation and control and three-layer composite film packaging. The ethylene concentration is reduced from 10 ppm to 2 ppm or below within 24 hours, and food respiration and microorganism breeding are inhibited; the intelligent sensing closed loop optimizes fresh-keeping parameters in real time, the freshness and shelf life of food are remarkably prolonged, deterioration loss is reduced, an automatic conveying and control system achieves full-process automation of material conveying, carrying and processing, a servo motor and a six-axis mechanical arm are matched, the positioning precision is high, the processing speed is high, manual intervention is reduced, and actual application and operation are facilitated.

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

The present invention relates to a composition for the prevention, alleviation, or treatment of mitochondrial complex 1 disorders, including a recombinant adeno-associated virus (AAV) vector loaded with Hydra NDi1. The inventors have confirmed that the scAAV-Hydra NDi1 of the present invention has an effect of restoring cellular respiration reduction caused by rotenone and an effect of inhibiting the apoptosis of LHON patient-derived cells by rotenone. Thus, the ScAAV-Hydra NDi1 is expected to be useful not only for LHON but also for preventing, alleviating, or treating diseases caused by abnormalities in mitochondrial complex 1.

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

The present invention addresses the problem of developing a drug for inhibiting the DNA damage restoration mechanism in a cancer which does not have a mutation in BRCA 1 / 2 and in which homologous recombination repair functions normally.　The inventors of the present invention have clarified that cell death can be induced by inhibiting PARP functions and mitochondrial respiration functions in cells, and indicated that the problem can be solved. In other words, the present invention provides: a method for inducing cell death by inhibiting PARP functions and mitochondrial respiration functions in cells; and a pharmaceutical composition for treating cancer, the pharmaceutical composition containing a PARP inhibitor and a mitochondrial respiration inhibitor.

The invention discloses preparation of a low-background-leakage microarray detection system for catalyzing a hairpin self-assembly reaction in combination with time-resolved resonance energy transfer and application of the microarray detection system in esophageal cancer diagnosis, and belongs to the technical field of biomedical detection. The probe combination provided by the invention comprises a probe pair for detecting miRNA-21, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.1 and SEQ ID NO.2; the nucleotide sequences of the probe pair for detecting the miRNA-25 are respectively SEQ ID NO.3 and SEQ ID NO.4; and / or a probe pair for detecting the miRNA-93, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.5 and SEQ ID NO.6. TMGA is added in a reaction system, the thermal stability of the probe H1 / H2 is enhanced through combination of positive ions of TMGA and DNA small grooves, so that background leakage caused by respiration is inhibited, a time-resolved fluorescence technology is adopted, and serum short-life autofluorescence is avoided by delaying measurement time.

The invention discloses an air control pressurization type cell culture instrument in the technical field of cell culture instruments. The air control pressurization type cell culture instrument comprises a culture cabin, a cabin cover and a pressure detection unit, the cabin cover is provided with a dynamic pressure simulation module which is used for generating periodic dynamic pressure for simulating the respiratory rhythm of a human body in the culture cabin; in the process of culturing the cells in the human body, the driving mechanism is used for driving the moving plate, the elastic element and the piston to reciprocate, the pressure in the culture cabin fluctuates within a certain range, and the periodic change of the in-vivo pressure intensity when the human body breathes is simulated, so that the cells grow in a state closer to the in-vivo state; a more reliable in-vitro cell model is provided for subsequent research for revealing physiological and pathological mechanisms of a human body; and in the culture process, the detection mechanism and the compensation mechanism are used for supplementing or discharging gas into the culture cabin, so that the air pressure change caused by cell respiration in the culture cabin can be automatically adjusted.

The present application belongs to the technical field of plant tissue culture, and particularly relates to an active ventilation tissue culture device and a use method. The tissue culture device comprises: a gas filling pump connected with a controller to control start and stop; a gas washing bottle with sterile water inside; an air inlet pipe of the gas washing bottle connected with the gas filling pump through an air guide pipe, and the other end of the air inlet pipe extends below the liquid level of the sterile water in the bottle; a plurality of tissue culture bottles connected in series by the air guide pipes; a tissue culture bottle at the end of the series connected by the air guide pipe for air exhaust; and air filters arranged on the air guide pipes between the gas washing bottle and the gas filling pump, on the air guide pipes between the tissue culture bottles, and on the air guide pipes for air exhaust. The serially connected passages make the air in the tissue culture bottles flow, reduce the accumulation of chemical substances, regulate and control the coordination between photosynthesis and respiration of the plants, and promote the normal and healthy growth of the plants.

The invention relates to the technical field of deep ground experiments, in particular to underground respiration-based sample lifting equipment which comprises a flow storage tank, the top of the flow storage tank is fixedly connected with an adjusting assembly, the top of the adjusting assembly is fixedly connected with a ground photovoltaic panel, the bottom of the flow storage tank is fixedly connected with a stratum control pipe, and the bottom of the stratum control pipe is fixedly connected with the ground photovoltaic panel. The bottom of the formation control pipe is fixedly connected with a fluid interaction pipe column, the outer wall of the formation control pipe is fixedly connected with a packing element, the fluid interaction pipe column is arranged and is of a cylindrical porous structure, and fluid is accurately screened by the pipe wall according to the hydrophilic and hydrophobic characteristics and the hydrophilic and hydrophobic air characteristics. When fluid is injected into the fluid storage tank, internal pressure of the fluid storage tank increases progressively to drive formation fluid in a drilling well to diffuse orderly to a shaft wall and a stratum with a specified depth through a porous pipe wall, pressure drop promotes the formation fluid to flow back to a pipe column when the fluid is recycled by the fluid storage tank, formation-shaft fluid interaction circulation is triggered, and the interaction process is completed.

The utility model provides a modularized rural small house type sewage treatment device with a porous microbial medium, which comprises the following structures: a plurality of front settling tanks, the water inlet sides of the front settling tanks are fixedly provided with water inlet pipes, and the opposite sides of two adjacent front settling tanks are respectively communicated with a water inlet and a water outlet of a water vapor exchange pipe; the water inlet end of the biochemical box is fixedly provided with an unpowered water spraying module, and the inner side of the biochemical box is fixedly provided with a plurality of porous microbial medium filler layers, so that the treatment efficiency is greatly improved; pollutants in domestic wastewater can be effectively decomposed, the cost is low, and the porous microbial medium filler can accelerate attachment and growth of microorganisms and maintain the activity of aerobic microorganisms under the unpowered condition.

The invention relates to the technical field of rice compositions, and discloses a composition for controlling rice sheath blight disease, which is prepared from the following raw materials in parts by weight: 5 to 15 parts of thifluzamide, 3 to 10 parts of epoxiconazole, 0.01 to 0.1 part of pimacol, 1.5 to 5 parts of suspending aid, 2 to 5 parts of dispersing aid, 8 to 15 parts of nutrition carrier and settling aid, 10 to 20 parts of filling agent and 0.1 to 0.5 part of anti-blocking agent. Two bactericides including thifluzamide and epoxiconazole are compounded with a sodium naphthalene acetate plant growth regulator, the thifluzamide prevents growth and reproduction of hyphae by inhibiting respiratory action of germs, and the epoxiconazole destroys cell membrane structures of the germs to kill the germs, has systemic property and is also effective for the germs invading into tissues; meanwhile, the sodium naphthalene acetate promotes the growth of rice roots, so that the rice sheath blight disease can be effectively controlled, the growth of the rice can be promoted on the whole, the stress resistance of the rice is improved, and the dual effects of preventing and treating diseases and promoting growth are achieved.

The invention discloses an inhibitor for inhibiting development of ampullaria gigas eggs and application of the inhibitor. Through medicament screening, a compound mixture of salad oil and acetic acid provided by the invention can effectively inhibit the development of ampullaria gigas egg masses, the optimal ratio of salad oil to acetic acid is 10: 1, and 0.07 g of medicament is applied to 1 g of eggs. Laboratory and field tests show that the inhibition rate of the mixture to ampullaria gigas egg masses reaches 100%. A scanning electron microscope is used for observing the processed ampullaria gigas ovum structure to show that more pores are formed on the surfaces of the ovum masses after the mixture is processed, so that the mixture can enter an embryo easily. Further respiratory action experiments show that the respiratory action of the egg masses treated by the mixture is obviously reduced within 24 hours, which is speculated that the mixture permeates into the egg masses, the respiratory action of the egg masses is inhibited, and the development of the eggs is further inhibited. The invention provides an environment-friendly, efficient and cheap technical means for prevention and treatment of ampullaria gigas.

The invention belongs to the technical field of cosmetics, discloses a moisturizing composition containing a Chinese herbal medicine compound, and particularly discloses a traditional Chinese medicine extract compound which comprises the following components: a radix ophiopogonis extract, a radix scrophulariae extract and a chlorella extract. The invention provides a traditional Chinese medicine extract compound prepared from radix ophiopogonis extract, radix scrophulariae extract and chlorella extract, and the traditional Chinese medicine extract compound not only can promote mitochondrial respiration, but also can promote generation of H2O in epidermal keratinocytes and dermal fibroblasts, so that generation of epidermal endogenous moisturizing factors and hyaluronic acid is promoted, and the skin moisturizing effect is improved. And the generation of hyaluronic acid in the dermis is increased, so that the comprehensive moisturizing function of the epidermis and the dermis can be maintained at the same time, and finally, the double-layer multi-moisturizing purpose of surface moisturizing and deep moisturizing is achieved.

The invention provides a litchi fresh-keeping and color-protecting preparation and a litchi fresh-keeping method, and belongs to the technical field of fruit fresh-keeping. The litchi fresh-keeping and color-protecting preparation comprises the following components in percentage by mass: 2%-8% of trehalose, 0.5%-2.5% of sodium bicarbonate and 0.2%-0.5% of a color-protecting preservative, the color-protecting preservative comprises the following components in parts by mass: 1-2 parts of ascorbic acid, 1-2 parts of D-sodium erythorbate and 1-2 parts of sodium tripolyphosphate. According to the method, after the litchi fresh-keeping and color-protecting preparation is adopted for pretreatment, a chitosan solution is adopted for film covering, so that the respiration, metabolism and enzyme activity of fresh fruits are inhibited, and water loss and shell browning are reduced; and after vacuum packaging, quickly freezing by using a liquid nitrogen quick freezing cabinet or a living cell partial freezing machine, storing, and unfreezing in different ways to obtain the litchis with relatively small color change, reduced water loss rate and high hardness.

Described herein is a new antidote for the rapid elimination of carbon monoxide from hemoglobin, including brain, heart, and red cell hemoglobin. The disclosed therapy involves the use of modified human globins, particularly neuroglobins modified at residue 64 and cytoglobins modified at residue 81, which bind carbon monoxide with extremely high affinity. The monomeric mutant globins are infused into blood, where they rapidly and irreversibly sequester carbon monoxide, and thus limit toxic effects of carbon monoxide on cellular respiration and oxygen transport and utilization.

The invention discloses a high-voltage electrostatic field keep-alive treatment device, and relates to the technical field of ice cream refrigeration, the high-voltage electrostatic field keep-alive treatment device comprises a pretreatment area, a main treatment area and a storage area which are sequentially connected in a sealed manner, the main treatment area is provided with a high-voltage electrostatic field keep-alive device, and the pretreatment area, the main treatment area and the storage area jointly form a tunnel type structure; a central processing unit is arranged in the tunnel type structure, the input end of the central processing unit is in communication connection with a multi-mode environment sensing module, and the output end of the central processing unit is in communication connection with an equipment cooperative control module. According to the application, the non-thermal biological effect of the high-voltage alternating electric field directly acts on functional raw material cells in the product, so that the cell membrane potential and the enzyme activity can be adjusted, and the abnormally vigorous respiration of the functional raw material cells in a cold storage environment is remarkably inhibited, so that the metabolism rate is slowed down; degradation and inactivation of active ingredients caused by metabolism acceleration are effectively prevented, and the nutritional function and biological activity of the active ingredients are reserved to the maximum extent.

To provide RcoM protein-based carbon monoxide scavengers and preparations for treating carbon monoxide poisoning.SOLUTION: Disclosed herein is a method for rapid elimination of carbon monoxide (CO) from CO-bound hemoglobin, myoglobin and cytochrome C oxidase in a subject with CO poisoning. The disclosed therapeutic method comprises use of a rationally designed and modified regulatory factor of CO metabolism (RcoM) protein and pharmaceutical compositions thereof, which scavenge carbon monoxide from poisoned tissue. The recombinant RcoM composition is infused into blood to rapidly sequester carbon monoxide and limit the toxic effects of carbon monoxide on cellular respiration, oxygen transport and oxygen utilization.SELECTED DRAWING: None

The present invention relates to a non-therapeutic cosmetic composition for increasing mitochondrial cellular respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising a complex of active ingredients: extracts of Melaleuca Alternifolia, extracts of Lavandula Hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition to increase mitochondrial cellular respiration of keratinocytes underlying the stratum corneum of the epidermis.

The present invention relates to a probiotic activity detection method. The probiotic activity detection method comprises: culturing a probiotic sample in a container, extracting gas in the container, detecting the gas using gas chromatography, calculating the CO2 concentration in the gas according to a standard curve, and calculating the probiotic activity in the sample using the CO2 concentration according to formula (1). The present invention designs a new probiotic activity detection method, deeply analyzes the respiration of probiotics, combines gas chromatography detection, constructs the relationship between the CO2 concentration in the culture gas and the bacterial activity, and realizes that the probiotic bacterial activity can be accurately evaluated by detecting the CO2 concentration in the culture gas, which is simple to operate and low in cost.

The invention provides a golden pomfret preservation method, which comprises: (1) preparing a preservation solution: adding tragacanth and chitosan into water, uniformly stirring and mixing, then adding a clausena lansium seed extract, a hypericum japonicum extract and a fig leaf extract, uniformly stirring and mixing, and finally adding glycerin, and uniformly stirring and mixing to obtain the preservation solution; and (2) performing fresh-keeping treatment on the golden pomfret, namely removing fish scales, gills and viscera from the fresh golden pomfret, washing the golden pomfret with clear water, soaking the golden pomfret in a fresh-keeping solution, draining the golden pomfret, and sub-packaging the golden pomfret subjected to fresh-keeping into food sealing bags for preservation. The fresh-keeping liquid is prepared from the clausena lansium seed extract, the herba hyperici japonici extract, the fig leaf extract and other raw materials, can keep good antibacterial performance, can also form a film to obstruct gas and reduce the respiratory action of fish cells, can also reduce the digestion degree of nutrient substances of fish and reduce the metabolic process, and has the effects of improving the immunity of the fish and improving the quality of the fish. And formation of active oxygen and peroxidation of membrane lipid are avoided, so that the fresh-keeping effect is improved, and the refrigeration shelf life is effectively prolonged.

The invention discloses a marine container nuclear track membrane fresh-keeping method which comprises the following steps: S1, respiration characteristic analysis and parameter determination: respiration characteristic parameters of goods to be transported are obtained, and the respiration characteristic parameters at least comprise the respiration rate of the goods under the aerobic condition, the respiration rate of the goods under the anaerobic condition and the ethylene release rate; and determining a target gas regulation and control range and a nuclear track membrane aperture parameter matched with the cargo type based on the breathing characteristic parameter and in combination with a basic fresh-keeping temperature of the cargo. And through the collaborative design of breathing characteristics, aperture parameters and installation layout, the precision of gas regulation and control is realized. The aperture of the nuclear track membrane is customized on the basis of parameters such as the cargo breathing rate and the ethylene release amount, the high selectivity of the monodisperse aperture of the nuclear track membrane is combined, O2 in the container can be stably controlled to be 2%-8%, CO2 can be controlled to be 3%-12%, ethylene is smaller than or equal to 0.1 ppm, the gas fluctuation range of the container is narrowed by 60% compared with that of a traditional controlled atmosphere container, and the breathing effect and the ethylene ripening effect are remarkably reduced.

The invention belongs to the technical field of gene engineering, and provides a LeMAN2 gene and an effect of the LeMAN2 gene in improvement of salt-tolerant breeding of tomatoes. The cDNA (complementary deoxyribonucleic acid) sequence of the LeMAN2 is as shown in SEQ ID NO. 1. The invention discovers that the tolerance of tomatoes under salt stress is improved by overexpression of the LeMAN2 gene. The cell damage degree of the LeMAN2 overexpression plant under the stress treatment condition of 100mmol / L NaCl is lower than that of the wild type (WT), and the emphasized expression is that the respiratory action intensity, the chlorophyll content and the activity of antioxidant enzymes (SOD, POD and CAT) are higher than those of the wild type plant; on the contrary, knockout of LeMAN2 reduces salt resistance of tomato plants. The results show that the overexpression of the LeMAN2 is beneficial to enhancing the salt adaptability of tomato plants, and the LeMAN2 is an important gene resource which can be utilized for developing salt-resistant biological breeding of tomatoes.