8 results about "Cellular respiration" patented technology

The present invention relates to an in vitro method for predicting the response to immunotherapy treatment of hepatocellular carcinoma in a subject, comprising the following steps: 1) A step of determining the energy metabolic state of a liver tissue sample from a subject, wherein the sample comprises pathological tissue and optionally non-pathological tissue, 2) A step of detecting the presence or absence of energy conversion resulting from a reduction in mitochondrial respiration in the pathological tissue by comparing it with a liver tissue sample of the subject or a non-pathological tissue from a standard non-pathological liver sample, Here: - If mitochondrial respiration is reduced in pathological tissue, the subject is less likely to respond to immunotherapy treatment, and - If mitochondrial respiration is not reduced in the pathological tissue, the subject is highly likely to respond to immunotherapy treatment.

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

The present invention addresses the problem of developing a drug for inhibiting the DNA damage restoration mechanism in a cancer which does not have a mutation in BRCA 1 / 2 and in which homologous recombination repair functions normally.　The inventors of the present invention have clarified that cell death can be induced by inhibiting PARP functions and mitochondrial respiration functions in cells, and indicated that the problem can be solved. In other words, the present invention provides: a method for inducing cell death by inhibiting PARP functions and mitochondrial respiration functions in cells; and a pharmaceutical composition for treating cancer, the pharmaceutical composition containing a PARP inhibitor and a mitochondrial respiration inhibitor.

The present application belongs to the technical field of plant tissue culture, and particularly relates to an active ventilation tissue culture device and a use method. The tissue culture device comprises: a gas filling pump connected with a controller to control start and stop; a gas washing bottle with sterile water inside; an air inlet pipe of the gas washing bottle connected with the gas filling pump through an air guide pipe, and the other end of the air inlet pipe extends below the liquid level of the sterile water in the bottle; a plurality of tissue culture bottles connected in series by the air guide pipes; a tissue culture bottle at the end of the series connected by the air guide pipe for air exhaust; and air filters arranged on the air guide pipes between the gas washing bottle and the gas filling pump, on the air guide pipes between the tissue culture bottles, and on the air guide pipes for air exhaust. The serially connected passages make the air in the tissue culture bottles flow, reduce the accumulation of chemical substances, regulate and control the coordination between photosynthesis and respiration of the plants, and promote the normal and healthy growth of the plants.

The invention discloses a marine container nuclear track membrane fresh-keeping method which comprises the following steps: S1, respiration characteristic analysis and parameter determination: respiration characteristic parameters of goods to be transported are obtained, and the respiration characteristic parameters at least comprise the respiration rate of the goods under the aerobic condition, the respiration rate of the goods under the anaerobic condition and the ethylene release rate; and determining a target gas regulation and control range and a nuclear track membrane aperture parameter matched with the cargo type based on the breathing characteristic parameter and in combination with a basic fresh-keeping temperature of the cargo. And through the collaborative design of breathing characteristics, aperture parameters and installation layout, the precision of gas regulation and control is realized. The aperture of the nuclear track membrane is customized on the basis of parameters such as the cargo breathing rate and the ethylene release amount, the high selectivity of the monodisperse aperture of the nuclear track membrane is combined, O2 in the container can be stably controlled to be 2%-8%, CO2 can be controlled to be 3%-12%, ethylene is smaller than or equal to 0.1 ppm, the gas fluctuation range of the container is narrowed by 60% compared with that of a traditional controlled atmosphere container, and the breathing effect and the ethylene ripening effect are remarkably reduced.

Aging progresses at different rates among individuals, underscoring the need for better indicators of functional decline. The inventors showed that the T cell energy metabolism in the elderly reveals that high mitochondrial oxidative phosphorylation, especially in CD4 T regulatory cells, is associated with a reduced probability of being frail / prefrail, independent of age, sex, or comorbidities. When mitochondrial respiration drops below a critical threshold, it adversely affects intrinsic capacity. Furthermore, a low level of oxidative phosphorylation in CD4 T helper cells, particularly in central and effector memory subsets, was significantly associated with a faster decline in intrinsic capacity over time, suggesting that T cell mitochondrial bioenergetics represents a key biomarker for managing functional decline in older adults. Accordingly, the present invention relates to a method for determining the risk of age-related functional decline in a subject comprising the step of measuring the mitochondrial dependency in a population of T cells wherein the mitochondrial dependency (MitoDep) correlates with the age-related functional decline.

A system and method for preserving tissues, organs, and fluids by controlling pressure and temperature to extend viability. It utilizes a pressure vessel to prevent ice crystal formation, thereby avoiding cell wall rupture and death. Cellular respiration is suspended, enhancing storage time. The design includes both mobile and stationary units, allowing for flexible application. The mobile unit operates with a 12-volt battery, while the stationary unit can hold multiple specimens. This innovation aims to maintain the viability of biological materials for medical use, significantly increasing their storage duration and utility.