20 results about "Cellular respiration" patented technology

The present invention relates to an in vitro method for predicting the response to immunotherapy treatment of hepatocellular carcinoma in a subject, comprising the following steps: 1) A step of determining the energy metabolic state of a liver tissue sample from a subject, wherein the sample comprises pathological tissue and optionally non-pathological tissue, 2) A step of detecting the presence or absence of energy conversion resulting from a reduction in mitochondrial respiration in the pathological tissue by comparing it with a liver tissue sample of the subject or a non-pathological tissue from a standard non-pathological liver sample, Here: - If mitochondrial respiration is reduced in pathological tissue, the subject is less likely to respond to immunotherapy treatment, and - If mitochondrial respiration is not reduced in the pathological tissue, the subject is highly likely to respond to immunotherapy treatment.

The utility model discloses integrated buried transfer station wastewater treatment equipment, relates to the field of wastewater treatment, and aims to solve the problems that in the prior art, when water in an anoxic bin is stirred, bubbles can be formed and gathered together to form foams due to the existence of a surfactant or other substances in the water, the respiration of microorganisms is influenced, and the sewage treatment effect is affected. In order to solve the problems of reduction of wastewater treatment efficiency, overflow of wastewater, need of additional treatment and cleaning and increase of treatment cost in the prior art, the utility model provides the following scheme: the device comprises a grating pool, one side of the grating pool is fixedly provided with an adjusting pool bin, an anoxic bin, an aerobic bin, a membrane pool, a clear water bin and an equipment room in sequence, and a basket grating is arranged in the grating pool; and a water passing hole is formed between the grating tank and the adjusting tank bin. According to the device, generated bubbles can be eliminated while the wastewater is stirred, so that the situation that the wastewater overflows, additional treatment and cleaning are needed, and the treatment cost is increased is avoided.

The present invention relates to a non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising an active complex: extracts of Melaleuca alternifolia, extracts of Lavandula hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis.

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

The present invention addresses the problem of developing a drug for inhibiting the DNA damage restoration mechanism in a cancer which does not have a mutation in BRCA 1 / 2 and in which homologous recombination repair functions normally.　The inventors of the present invention have clarified that cell death can be induced by inhibiting PARP functions and mitochondrial respiration functions in cells, and indicated that the problem can be solved. In other words, the present invention provides: a method for inducing cell death by inhibiting PARP functions and mitochondrial respiration functions in cells; and a pharmaceutical composition for treating cancer, the pharmaceutical composition containing a PARP inhibitor and a mitochondrial respiration inhibitor.

The invention discloses preparation of a low-background-leakage microarray detection system for catalyzing a hairpin self-assembly reaction in combination with time-resolved resonance energy transfer and application of the microarray detection system in esophageal cancer diagnosis, and belongs to the technical field of biomedical detection. The probe combination provided by the invention comprises a probe pair for detecting miRNA-21, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.1 and SEQ ID NO.2; the nucleotide sequences of the probe pair for detecting the miRNA-25 are respectively SEQ ID NO.3 and SEQ ID NO.4; and / or a probe pair for detecting the miRNA-93, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.5 and SEQ ID NO.6. TMGA is added in a reaction system, the thermal stability of the probe H1 / H2 is enhanced through combination of positive ions of TMGA and DNA small grooves, so that background leakage caused by respiration is inhibited, a time-resolved fluorescence technology is adopted, and serum short-life autofluorescence is avoided by delaying measurement time.

The invention discloses an air control pressurization type cell culture instrument in the technical field of cell culture instruments. The air control pressurization type cell culture instrument comprises a culture cabin, a cabin cover and a pressure detection unit, the cabin cover is provided with a dynamic pressure simulation module which is used for generating periodic dynamic pressure for simulating the respiratory rhythm of a human body in the culture cabin; in the process of culturing the cells in the human body, the driving mechanism is used for driving the moving plate, the elastic element and the piston to reciprocate, the pressure in the culture cabin fluctuates within a certain range, and the periodic change of the in-vivo pressure intensity when the human body breathes is simulated, so that the cells grow in a state closer to the in-vivo state; a more reliable in-vitro cell model is provided for subsequent research for revealing physiological and pathological mechanisms of a human body; and in the culture process, the detection mechanism and the compensation mechanism are used for supplementing or discharging gas into the culture cabin, so that the air pressure change caused by cell respiration in the culture cabin can be automatically adjusted.

The present application belongs to the technical field of plant tissue culture, and particularly relates to an active ventilation tissue culture device and a use method. The tissue culture device comprises: a gas filling pump connected with a controller to control start and stop; a gas washing bottle with sterile water inside; an air inlet pipe of the gas washing bottle connected with the gas filling pump through an air guide pipe, and the other end of the air inlet pipe extends below the liquid level of the sterile water in the bottle; a plurality of tissue culture bottles connected in series by the air guide pipes; a tissue culture bottle at the end of the series connected by the air guide pipe for air exhaust; and air filters arranged on the air guide pipes between the gas washing bottle and the gas filling pump, on the air guide pipes between the tissue culture bottles, and on the air guide pipes for air exhaust. The serially connected passages make the air in the tissue culture bottles flow, reduce the accumulation of chemical substances, regulate and control the coordination between photosynthesis and respiration of the plants, and promote the normal and healthy growth of the plants.

The invention discloses a high-voltage electrostatic field keep-alive treatment device, and relates to the technical field of ice cream refrigeration, the high-voltage electrostatic field keep-alive treatment device comprises a pretreatment area, a main treatment area and a storage area which are sequentially connected in a sealed manner, the main treatment area is provided with a high-voltage electrostatic field keep-alive device, and the pretreatment area, the main treatment area and the storage area jointly form a tunnel type structure; a central processing unit is arranged in the tunnel type structure, the input end of the central processing unit is in communication connection with a multi-mode environment sensing module, and the output end of the central processing unit is in communication connection with an equipment cooperative control module. According to the application, the non-thermal biological effect of the high-voltage alternating electric field directly acts on functional raw material cells in the product, so that the cell membrane potential and the enzyme activity can be adjusted, and the abnormally vigorous respiration of the functional raw material cells in a cold storage environment is remarkably inhibited, so that the metabolism rate is slowed down; degradation and inactivation of active ingredients caused by metabolism acceleration are effectively prevented, and the nutritional function and biological activity of the active ingredients are reserved to the maximum extent.

The present invention relates to a non-therapeutic cosmetic composition for increasing mitochondrial cellular respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising a complex of active ingredients: extracts of Melaleuca Alternifolia, extracts of Lavandula Hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition to increase mitochondrial cellular respiration of keratinocytes underlying the stratum corneum of the epidermis.

The invention discloses a marine container nuclear track membrane fresh-keeping method which comprises the following steps: S1, respiration characteristic analysis and parameter determination: respiration characteristic parameters of goods to be transported are obtained, and the respiration characteristic parameters at least comprise the respiration rate of the goods under the aerobic condition, the respiration rate of the goods under the anaerobic condition and the ethylene release rate; and determining a target gas regulation and control range and a nuclear track membrane aperture parameter matched with the cargo type based on the breathing characteristic parameter and in combination with a basic fresh-keeping temperature of the cargo. And through the collaborative design of breathing characteristics, aperture parameters and installation layout, the precision of gas regulation and control is realized. The aperture of the nuclear track membrane is customized on the basis of parameters such as the cargo breathing rate and the ethylene release amount, the high selectivity of the monodisperse aperture of the nuclear track membrane is combined, O2 in the container can be stably controlled to be 2%-8%, CO2 can be controlled to be 3%-12%, ethylene is smaller than or equal to 0.1 ppm, the gas fluctuation range of the container is narrowed by 60% compared with that of a traditional controlled atmosphere container, and the breathing effect and the ethylene ripening effect are remarkably reduced.

The utility model relates to the technical field of preservation, in particular to a beet ventilation preservation device which comprises a main air pipe, a branch air pipe and an air blower, the main air pipe comprises a plurality of first splicing pipes, and the adjacent first splicing pipes are communicated and connected through first splicing pieces; a first splicing pipe at one end of the main air pipe is communicated with the air outlet end of the air blower; each branch air pipe comprises a plurality of second splicing pipes, a plurality of air guide holes are uniformly formed in the second splicing pipes at intervals, the adjacent second splicing pipes are communicated and connected through second splicing pieces, a mounting frame is erected between every two adjacent branch air pipes, the mounting frame is provided with a mounting pipe, and the mounting pipe is provided with a temperature sensor; the temperature sensor is electrically connected with the air blower and the electric air door through the controller. According to the beet ventilation preservation device, the temperature in a beet pile can be reduced, so that the respiration of beets is reduced, the reproduction of related microorganisms is controlled, and the sugar loss of the beets is reduced in the preservation period.

The application relates to the technical field of plastic materials, in particular to a high-permeability puncture-resistant film material, a preparation method and application in rice planting. The film material is prepared by taking PBAT as a base material, and the PBAT base film material has the characteristics of good biodegradability and high water vapor permeability; a toughening material is introduced into the PBAT, which can effectively provide the toughness of the film material, and further improve the puncture resistance of the film material; the toughening material includes organic toughening material and inorganic toughening material, the organic toughening material and the inorganic toughening material are connected to polylactic acid molecules through stable chemical bonds, and the toughening effect is good; the inorganic toughening material, i.e. silicon-based mesoporous material, has a porous structure, small permeation resistance to gas, is beneficial to the diffusion and transmission of gas, and can improve the permeability of the film material. When the film material is applied to rice planting, not only can the temperature be increased and the water be preserved, but also the nutrient exchange, respiration and photosynthesis of rice seedlings can be efficiently carried out.

Aging progresses at different rates among individuals, underscoring the need for better indicators of functional decline. The inventors showed that the T cell energy metabolism in the elderly reveals that high mitochondrial oxidative phosphorylation, especially in CD4 T regulatory cells, is associated with a reduced probability of being frail / prefrail, independent of age, sex, or comorbidities. When mitochondrial respiration drops below a critical threshold, it adversely affects intrinsic capacity. Furthermore, a low level of oxidative phosphorylation in CD4 T helper cells, particularly in central and effector memory subsets, was significantly associated with a faster decline in intrinsic capacity over time, suggesting that T cell mitochondrial bioenergetics represents a key biomarker for managing functional decline in older adults. Accordingly, the present invention relates to a method for determining the risk of age-related functional decline in a subject comprising the step of measuring the mitochondrial dependency in a population of T cells wherein the mitochondrial dependency (MitoDep) correlates with the age-related functional decline.

The present invention relates to a composition and the like for preventing, ameliorating or treating mitochondrial complex 1 abnormality diseases, the composition comprising a hydranth (Hydra NDi1-carrying recombinant adeno-associated virus vector. The inventors have confirmed that scAAV-hydranth NDi1 according to the present invention has an effect of restoring reduced respiration of cells caused by rotenone and an effect of inhibiting apoptosis of LHON patient-derived cells caused by rotenone, and thus it is expected to be used in the prevention, amelioration or treatment of LHON and diseases caused by mitochondrial complex 1 abnormalities.

A system and method for preserving tissues, organs, and fluids by controlling pressure and temperature to extend viability. It utilizes a pressure vessel to prevent ice crystal formation, thereby avoiding cell wall rupture and death. Cellular respiration is suspended, enhancing storage time. The design includes both mobile and stationary units, allowing for flexible application. The mobile unit operates with a 12-volt battery, while the stationary unit can hold multiple specimens. This innovation aims to maintain the viability of biological materials for medical use, significantly increasing their storage duration and utility.

A method of increasing mitochondrial respiration in a cell makes use of a compound having the structure of formula (I) or formula (II).

Methods for rapid removal of carbon monoxide (CO) from CO-bound hemoglobin, myoglobin, and cytochrome c oxidase in a subject poisoned with CO are described. The disclosed therapy involves the use of a rationally designed, modified CO metabolic regulator (RcoM) protein and pharmaceutical compositions thereof that clear carbon monoxide from the poisoned tissue. Recombinant RcoM compositions are infused into the blood, where they rapidly sequester carbon monoxide and limit the toxic effects of carbon monoxide on cellular respiration, oxygen delivery, and oxygen utilization.

This disclosure relates to methods of non-invasively measuring the degree of cellular energy metabolism. The methods disclosed herein comprise applying monitoring devices to a bodily surface or solution containing cells (e.g., a dermal surface of a subject), generating a radio frequency, detecting operating values of the circuitry of the device, maintaining contact between the monitoring device and the bodily surface or solution containing cells for a desired length of time, and collecting data that corresponds to the degree of cellular energy metabolism. Methods of this disclosure can be used to correlate the degree of cellular energy metabolism to other physiological or cellular phenomena (e.g., cellular respiration, cell viability).