18 results about "Deep tissue imaging" patented technology

The present application relates to a kind of electron donor (alpha-trithiophene) modified rhodamine dye, its synthesis method and application.The dye has strong absorption of 500-600 nm, can emit efficiently in 550-700 nm band, suitable for deep tissue imaging.The structure endows mitochondria targeting ability, can be used as biomarker probe to realize subcellular organelle accurate positioning;Meanwhile, under light, it can efficiently produce reactive oxygen species through type I mechanism, suitable for the photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes.In addition, the dye can also be used as organic photocatalyst for environmental or energy-related photocatalytic reaction.The present application provides a new choice for multi-scene optoelectronic functional materials.

The application relates to a kind of self-adapting scattering deep penetration microscopic endoscopic imaging method, device and medium, method comprises the following steps: S1, the input-output mapping relationship of optical fiber under specified penetration depth is measured;S2, when the same specified penetration depth is set with biological sample, effective pixel is traversed;S3, when traversing each effective pixel, the traversal range of the phase constant of each pixel is adjusted, and the phase constant corresponding to each pixel is obtained, so that the light intensity is maximum;S4, data integration is carried out, and a group of self-adapting scattering matrix corresponding to each pixel is obtained;S5, calling self-adapting scattering matrix as phase parameter, scanning is carried out pixel by pixel, and the light intensity corresponding to pixel is obtained, and the light intensity is combined into a frame gray scale image.Compared with prior art, the application has the advantages of clearly imaging deep tissue, effectively improving imaging quality and the like.

The invention discloses a rare earth homologous dual-emission high-resolution deep imaging method based on deep learning enhancement. A plurality of long-life intermediate states of lanthanide upconversion nanoparticles (UCNPs) are utilized, a single 980nm pumping source is used for exciting Tm < 3 + > / Yb < 3 + > co-doped UCNPs, and two-photon fluorescence with higher penetrability and four-photon fluorescence with higher resolution are induced at the same time. By combining a dual-mechanism CycleGAN artificial neural network based on antagonism training and cyclic consistency constraint, cross-domain mapping between two fluorescence signals is established, and the advantages of two fluorescence imaging are cooperatively utilized, so that deep penetration and high-resolution imaging are realized. According to the invention, in a self-established wide-field microscopic imaging system, the resolution of 209nm of a single particle in a 808nm fluorescence channel can be realized, and the signal-to-background ratio is doubled. According to the invention, the technical bottleneck that the resolution and the penetration depth of a traditional multi-photon microscope in deep tissue imaging are mutually restricted is effectively solved.

A multiplexing adaptive optics method for deep-tissue imaging is provided, comprising: providing a first optical beam and a group of second optical beams, each with lower intensity and distinct modulation frequencies; combining the beams with temporal overlap to form a composite excitation beam; focusing the composite beam into a sample such that the second optical beams are laterally separated in the focal plane; detecting a signal excited by the composite beam; demodulating the signal with reference frequencies to compute phase and amplitude data at each modulation frequency and obtain a second signal; performing spatial measurements of the first beam relative to the second beams to derive down-sampled electric-field point spread functions; and merging the functions to reconstruct a full-sampled point spread function for identifying and correcting optical aberrations within the sample.

The invention provides a bimodal OCT-FBG optical fiber probe and a temperature measurement and imaging system and method.The bimodal OCT-FBG optical fiber probe comprises an integrated optical fiber probe and a protective shell, and the integrated optical fiber probe is arranged in the protective shell; the integrated optical fiber probe comprises a single-mode optical fiber, a graded-index optical fiber and a coreless optical fiber which are welded from the tail part to the end part in sequence; a Bragg grating is written into the core layer of the single-mode fiber. According to the dual-mode OCT-FBG optical fiber probe, the problem that the temperature of an existing probe is combined with OCT imaging is solved, the temperature measuring function and the OCT imaging function are combined in one optical fiber probe, and temperature data can be obtained while OCT imaging information is obtained. The device has the possibility of being applied to various occasions, for example, the ablation effect is monitored in the laser ablation process, and overheating is prevented; and deep tissue imaging, temperature measurement and the like.

This invention belongs to the field of analytical chemistry and relates to the preparation and sensing applications of highly efficient electrochemiluminescent gold nanoclusters protected by co-reactive ligands. This invention synthesizes a class of gold nanoclusters protected by co-reactive ligands, whose ligand molecular backbone includes terminal thiol groups as anchoring groups and a tertiary amine structure. Using co-reactive ligands as stabilizers, a one-pot method is employed to synthesize highly efficient electrochemiluminescent gold nanoclusters. This method not only stabilizes the gold nanoclusters, preventing them from agglomerating and losing their optical properties, but also utilizes the tertiary amine structure to function as a co-reactant. This allows the synthesized gold nanoclusters to generate highly efficient anodic near-infrared electrochemiluminescence without the participation of exogenous co-reactants, providing an opportunity for the preparation of highly sensitive and convenient sensors, high-brightness organic light-emitting diodes, and deep tissue imaging research. Simultaneously, it enables highly sensitive, highly specific, and convenient detection of carboxylesterases, providing a new method for the early clinical diagnosis of hepatocellular carcinoma.

The invention relates to the technical field of chemiluminescent probes, and discloses a laser-activated near-infrared two-region chemiluminescent molecule and a preparation method thereof, and the molecular structural formula of the laser-activated near-infrared two-region chemiluminescent molecule is shown in the specification. The near-infrared two-region chemiluminescent molecule provided by the invention not only has a high signal-to-noise ratio (SBR) of near-infrared two-region chemiluminescence, but also responds to beta-galactase in a tumor microenvironment, subsequently realizes photoacoustic activated deep tissue imaging through laser irradiation, and selectively opens a signal in a specific tumor focus to achieve a treatment effect on tumors.

The invention belongs to the technical field of fluorescent probes, and particularly discloses an N-CDs fluorescent probe as well as a preparation method and application thereof, and the fluorescent probe is prepared from p-phenylenediamine and L-tartaric acid by adopting a one-step hydrothermal method. The invention relates to a carbon quantum dot probe which has a two-photon absorption characteristic and can specifically target a cell nucleus and a lipid droplet organelle in a living cell at the same time so as to realize double-color and double-target fluorescence imaging. The probe has important application value in the aspects of long-time dynamic observation of living cells, multi-organelle interaction research and deep tissue imaging.

The present application relates to a kind of side chain modified pentamethine cyanine dye and its synthesis method and application.The dye has general formula CY5-R, the dye has strong absorption and emission characteristics above 650nm, suitable for red light excitation, mitochondrial localization and type I photodynamic therapy.Experiments show that the dye and mitochondrial co-localization coefficient reaches 0.860, exhibit excellent deep tissue penetration ability and low dark toxicity, after illumination IC 50 0.15 μM, can efficiently generate superoxide anion free radical, significantly kill cancer cells, realize diagnosis and treatment integration.The synthesis method of the present application includes quaternary ammonium salt synthesis, condensation reaction and side chain modification and the like, with the characteristics of cheap raw material, high yield, simple operation.The dye has wide application in photocatalysis, photodynamic therapy, biomarker and deep tissue imaging and the like, especially suitable for deep tumor fluorescence imaging and photodynamic therapy.

The invention belongs to the technical field of bioengineering and molecular imaging, and particularly relates to a novel multi-excitation fluorescent protein and application thereof, the nucleotide sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.1, and the amino acid sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.2. The multi-excitation fluorescent protein disclosed by the invention effectively overcomes the limitation that the protein or a light source needs to be frequently replaced due to narrow excitation spectrum in multiple imaging of the traditional fluorescent protein, so that multi-mode and multi-parameter imaging by using the same protein in a single experiment becomes possible. According to the invention, the experimental process is greatly simplified, the experimental cost is reduced, the application range of the method in the fields of dynamic tracking of living cells, simultaneous detection of multiple components, deep tissue imaging and the like is remarkably widened, and a more powerful and flexible tool is provided for life science research and medical diagnosis.

The invention discloses a nano composite material for emitting light and measuring temperature in a near-infrared NIR-IIb region as well as a preparation method and application of the nano composite material. Raw materials of the composite material comprise rare earth nanoparticles and distearoyl phosphatidyl ethanolamine-polyethylene glycol, the rare earth nanoparticles comprise a core layer and a shell layer coating the core layer, the structural general formula of the core layer is NaEr1-ATmAF4, the structural general formula of the shell layer is NaLuF4, and A is larger than or equal to 0 and smaller than or equal to 0.1. The nano composite material disclosed by the invention is high in temperature measurement repeatability and good in temperature measurement stability, a luminescence emission peak is positioned in an optimal imaging region (NIR-IIb), and the imaging resolution of deep tissues can be remarkably enhanced. Therefore, the composite material disclosed by the invention becomes an ideal material for promoting the development of a living body deep tissue high-precision temperature measurement imaging technology based on the unique luminescence characteristic and stable temperature measurement performance, and has huge application potential in the fields of biomedical imaging and temperature measurement.

The invention relates to an electron donor (alpha-trithiophene) modified rhodamine dye as well as a synthesis method and application thereof. The dye has strong absorption of 500-600 nm, can efficiently emit in a wave band of 550-700 nm, and is suitable for deep tissue imaging. The structure endows mitochondria with targeting ability, and the probe can be used as a biomarker probe to realize precise positioning of subcellular organelles; meanwhile, active oxygen is efficiently generated through an I-type mechanism under illumination, and the compound is suitable for photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes. In addition, the dye can also be used as an organic photocatalyst for environment or energy related photocatalytic reaction. The invention provides a new choice for a multi-scene photoelectric functional material.

The invention discloses an Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material as well as a preparation method and application thereof, and relates to the technical field of luminescent materials. The chemical formula of the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material is Zn < 2-x > Er < x > Ti0. 5Sn0. 5O4: yCr < 3 + >, x is greater than or equal to 0.005 and less than or equal to 0.1, and y is greater than or equal to 0.005 and less than or equal to 0.1; the specific preparation method comprises the following steps: weighing raw materials of SnO2, ZnO, TiO2, Cr2O3 and Er2O3 according to the molar ratio of the chemical formula, mixing, adding an organic solvent, and grinding to obtain homogeneous powder; and calcining the homogeneous powder to obtain the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder. According to the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material prepared by the preparation method disclosed by the invention, Er < 3 + > and Cr < 3 + > are co-doped into a Zn2Ti0. 5Sn0. 5O4 matrix to realize a synergistic effect under the excitation of 465nm blue light, broadband near-infrared emission taking 800nm as a center is enhanced, the coverage range of an emission spectrum is wider, and the intensity is higher; the blue light excited NIR pc-LED device is suitable for constructing blue light excited NIR pc-LED devices, shows high penetrability and signal-to-noise ratio in night vision imaging and biomedical deep tissue imaging, and can be applied to near infrared imaging.

The invention belongs to the technical field of fluorescent small molecular dyes, and particularly relates to a near-infrared fluorescent dye, a preparation method and application thereof, and construction and application of a parent nucleus structure of a high-performance near-infrared fluorescent dye. The near-infrared fluorescent dye has a fluorescent dye mother nucleus with large Stokes shift, has long absorption and emission wavelength, has the Stokes shift exceeding 100 nm, has good light stability and chemical stability, can be used for modularization synthesis of dyes, and has modifiability. The fluorescent dye molecule can target and locate mitochondria, is used for cell imaging, has good cell light stability, and can be further used for organism deep tissue imaging.

This invention discloses a computationally adaptive aberration correction imaging method for deep tissue based on upconversion nanoparticle guide stars. A near-infrared fluorescence imaging system is constructed using rare-earth upconversion nanoparticles as guide stars. Fluorescence distortion images of deep tissue samples are acquired through near-infrared excitation, and imaging aberrations are parameterized based on Zernike polynomials. Aberration coefficients are reconstructed using a deep learning model incorporating physical prior constraints. Digital wavefront compensation is performed based on the reconstructed aberration information to achieve deep tissue aberration correction and obtain high-resolution imaging results. This invention eliminates the need for complex optical hardware such as wavefront sensors or deformable mirrors, and can stably achieve aberration estimation and compensation even in 360 μm deep tissue conditions. Compared with traditional aberration correction methods, the correction effect is improved by more than 30%, enabling rapid image correction and high-precision positioning. It solves the problems of weak guide star signals, low aberration correction efficiency, and high system cost in deep tissue imaging, and is applicable to deep tissue optical imaging in fields such as clinical diagnosis, biological cell observation, and drug development.

The invention provides a bladder volume measuring probe and system based on a two-dimensional ultrasonic array, and relates to the field of medical ultrasonic noninvasive detection. An ultrasonic module is arranged in the bladder volume measuring probe and comprises N * M ultrasonic transducer array elements forming a two-dimensional ultrasonic area array or N + M ultrasonic transducer array elements forming a row and column addressing ultrasonic array; the distance between the adjacent ultrasonic transducer array elements is (0.2-0.7) lambda mm, and lambda is the wavelength corresponding to the center frequency of the ultrasonic transducer array elements. An ultrasonic area array formed by N * M ultrasonic transducer array elements or a row-column addressing array formed by N + M ultrasonic transducer array elements is creatively adopted, and meanwhile, a dynamic focusing technology is configured, so that the delay control precision is superior to 10ns, the imaging resolution of bladder walls with different depths is effectively improved, and the imaging precision of bladder walls with different depths is improved. The technical problem that imaging quality of a traditional ultrasonic probe in shallow and deep tissues is difficult to consider at the same time is solved, and portability, accuracy and non-AI dependence are met at the same time.

The application discloses a ratio type near-infrared fluorescent probe based on an ICT-FRET mechanism and a preparation method and application thereof. The ratio type near-infrared fluorescent probe based on the ICT-FRET mechanism emits in a near-infrared region, has an ultra-large stokes shift, can effectively avoid excitation light interference and self-absorption, reduces biological background fluorescence, and is suitable for deep tissue imaging. The probe can realize double-emission ratio response (I 660 / I 540 ) based on the FRET mechanism, has a significant signal change, has inherent self-calibration capability, shows extremely high selectivity to Cys, has almost no response to Hcy and GSH similar in structure, is strong in anti-environmental interference, and is more accurate in quantification. The TPP targeting group of the probe enables the probe to be specifically positioned in mitochondria of living cells, realizes dynamic ratio imaging of endogenous and exogenous Cys in mitochondria, and provides a powerful tool for studying Cys metabolism at a subcellular level.

This invention relates to the field of biodetection technology, specifically to a NO-responsive near-infrared II fluorescent probe, its preparation method, and its application. The structure of the fluorescent probe is shown below. The fluorescent probe TD-OT-BNH2 of this invention exhibits high sensitivity to NO and a low detection limit, meeting the needs for trace NO detection. This fluorescent probe demonstrates good selectivity and anti-interference ability against common metal ions, anions, biothiols, and reactive oxygen species. After reacting with NO, both the absorption and emission spectra of the fluorescent probe undergo a significant redshift, generating a clear fluorescence signal within the NIR-II imaging window, suitable for deep tissue imaging. The fluorescent probe possesses aggregation-induced emission properties, which enhances its fluorescence imaging performance in complex biological environments. This fluorescent probe exhibits low cytotoxicity and good biocompatibility, and can be used for visual detection of nitric oxide at the cellular and in vivo levels.