11 results about "Deep tissue imaging" patented technology

The present application relates to a kind of electron donor (alpha-trithiophene) modified rhodamine dye, its synthesis method and application.The dye has strong absorption of 500-600 nm, can emit efficiently in 550-700 nm band, suitable for deep tissue imaging.The structure endows mitochondria targeting ability, can be used as biomarker probe to realize subcellular organelle accurate positioning;Meanwhile, under light, it can efficiently produce reactive oxygen species through type I mechanism, suitable for the photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes.In addition, the dye can also be used as organic photocatalyst for environmental or energy-related photocatalytic reaction.The present application provides a new choice for multi-scene optoelectronic functional materials.

A multiplexing adaptive optics method for deep-tissue imaging is provided, comprising: providing a first optical beam and a group of second optical beams, each with lower intensity and distinct modulation frequencies; combining the beams with temporal overlap to form a composite excitation beam; focusing the composite beam into a sample such that the second optical beams are laterally separated in the focal plane; detecting a signal excited by the composite beam; demodulating the signal with reference frequencies to compute phase and amplitude data at each modulation frequency and obtain a second signal; performing spatial measurements of the first beam relative to the second beams to derive down-sampled electric-field point spread functions; and merging the functions to reconstruct a full-sampled point spread function for identifying and correcting optical aberrations within the sample.

The invention relates to the technical field of chemiluminescent probes, and discloses a laser-activated near-infrared two-region chemiluminescent molecule and a preparation method thereof, and the molecular structural formula of the laser-activated near-infrared two-region chemiluminescent molecule is shown in the specification. The near-infrared two-region chemiluminescent molecule provided by the invention not only has a high signal-to-noise ratio (SBR) of near-infrared two-region chemiluminescence, but also responds to beta-galactase in a tumor microenvironment, subsequently realizes photoacoustic activated deep tissue imaging through laser irradiation, and selectively opens a signal in a specific tumor focus to achieve a treatment effect on tumors.

The invention belongs to the technical field of fluorescent probes, and particularly discloses an N-CDs fluorescent probe as well as a preparation method and application thereof, and the fluorescent probe is prepared from p-phenylenediamine and L-tartaric acid by adopting a one-step hydrothermal method. The invention relates to a carbon quantum dot probe which has a two-photon absorption characteristic and can specifically target a cell nucleus and a lipid droplet organelle in a living cell at the same time so as to realize double-color and double-target fluorescence imaging. The probe has important application value in the aspects of long-time dynamic observation of living cells, multi-organelle interaction research and deep tissue imaging.

The present application relates to a kind of side chain modified pentamethine cyanine dye and its synthesis method and application.The dye has general formula CY5-R, the dye has strong absorption and emission characteristics above 650nm, suitable for red light excitation, mitochondrial localization and type I photodynamic therapy.Experiments show that the dye and mitochondrial co-localization coefficient reaches 0.860, exhibit excellent deep tissue penetration ability and low dark toxicity, after illumination IC 50 0.15 μM, can efficiently generate superoxide anion free radical, significantly kill cancer cells, realize diagnosis and treatment integration.The synthesis method of the present application includes quaternary ammonium salt synthesis, condensation reaction and side chain modification and the like, with the characteristics of cheap raw material, high yield, simple operation.The dye has wide application in photocatalysis, photodynamic therapy, biomarker and deep tissue imaging and the like, especially suitable for deep tumor fluorescence imaging and photodynamic therapy.

The invention belongs to the technical field of bioengineering and molecular imaging, and particularly relates to a novel multi-excitation fluorescent protein and application thereof, the nucleotide sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.1, and the amino acid sequence of the novel multi-excitation fluorescent protein is shown as SEQ ID NO.2. The multi-excitation fluorescent protein disclosed by the invention effectively overcomes the limitation that the protein or a light source needs to be frequently replaced due to narrow excitation spectrum in multiple imaging of the traditional fluorescent protein, so that multi-mode and multi-parameter imaging by using the same protein in a single experiment becomes possible. According to the invention, the experimental process is greatly simplified, the experimental cost is reduced, the application range of the method in the fields of dynamic tracking of living cells, simultaneous detection of multiple components, deep tissue imaging and the like is remarkably widened, and a more powerful and flexible tool is provided for life science research and medical diagnosis.

The invention relates to an electron donor (alpha-trithiophene) modified rhodamine dye as well as a synthesis method and application thereof. The dye has strong absorption of 500-600 nm, can efficiently emit in a wave band of 550-700 nm, and is suitable for deep tissue imaging. The structure endows mitochondria with targeting ability, and the probe can be used as a biomarker probe to realize precise positioning of subcellular organelles; meanwhile, active oxygen is efficiently generated through an I-type mechanism under illumination, and the compound is suitable for photodynamic regulation and biosensing for non-disease diagnosis and treatment purposes. In addition, the dye can also be used as an organic photocatalyst for environment or energy related photocatalytic reaction. The invention provides a new choice for a multi-scene photoelectric functional material.

The invention discloses an Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material as well as a preparation method and application thereof, and relates to the technical field of luminescent materials. The chemical formula of the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material is Zn < 2-x > Er < x > Ti0. 5Sn0. 5O4: yCr < 3 + >, x is greater than or equal to 0.005 and less than or equal to 0.1, and y is greater than or equal to 0.005 and less than or equal to 0.1; the specific preparation method comprises the following steps: weighing raw materials of SnO2, ZnO, TiO2, Cr2O3 and Er2O3 according to the molar ratio of the chemical formula, mixing, adding an organic solvent, and grinding to obtain homogeneous powder; and calcining the homogeneous powder to obtain the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder. According to the Er < 3 + > / Cr < 3 + > co-doped Zn2Ti0. 5Sn0. 5O4 near-infrared fluorescent powder material prepared by the preparation method disclosed by the invention, Er < 3 + > and Cr < 3 + > are co-doped into a Zn2Ti0. 5Sn0. 5O4 matrix to realize a synergistic effect under the excitation of 465nm blue light, broadband near-infrared emission taking 800nm as a center is enhanced, the coverage range of an emission spectrum is wider, and the intensity is higher; the blue light excited NIR pc-LED device is suitable for constructing blue light excited NIR pc-LED devices, shows high penetrability and signal-to-noise ratio in night vision imaging and biomedical deep tissue imaging, and can be applied to near infrared imaging.

This invention discloses a computationally adaptive aberration correction imaging method for deep tissue based on upconversion nanoparticle guide stars. A near-infrared fluorescence imaging system is constructed using rare-earth upconversion nanoparticles as guide stars. Fluorescence distortion images of deep tissue samples are acquired through near-infrared excitation, and imaging aberrations are parameterized based on Zernike polynomials. Aberration coefficients are reconstructed using a deep learning model incorporating physical prior constraints. Digital wavefront compensation is performed based on the reconstructed aberration information to achieve deep tissue aberration correction and obtain high-resolution imaging results. This invention eliminates the need for complex optical hardware such as wavefront sensors or deformable mirrors, and can stably achieve aberration estimation and compensation even in 360 μm deep tissue conditions. Compared with traditional aberration correction methods, the correction effect is improved by more than 30%, enabling rapid image correction and high-precision positioning. It solves the problems of weak guide star signals, low aberration correction efficiency, and high system cost in deep tissue imaging, and is applicable to deep tissue optical imaging in fields such as clinical diagnosis, biological cell observation, and drug development.

The application discloses a ratio type near-infrared fluorescent probe based on an ICT-FRET mechanism and a preparation method and application thereof. The ratio type near-infrared fluorescent probe based on the ICT-FRET mechanism emits in a near-infrared region, has an ultra-large stokes shift, can effectively avoid excitation light interference and self-absorption, reduces biological background fluorescence, and is suitable for deep tissue imaging. The probe can realize double-emission ratio response (I 660 / I 540 ) based on the FRET mechanism, has a significant signal change, has inherent self-calibration capability, shows extremely high selectivity to Cys, has almost no response to Hcy and GSH similar in structure, is strong in anti-environmental interference, and is more accurate in quantification. The TPP targeting group of the probe enables the probe to be specifically positioned in mitochondria of living cells, realizes dynamic ratio imaging of endogenous and exogenous Cys in mitochondria, and provides a powerful tool for studying Cys metabolism at a subcellular level.

This invention relates to the field of biodetection technology, specifically to a NO-responsive near-infrared II fluorescent probe, its preparation method, and its application. The structure of the fluorescent probe is shown below. The fluorescent probe TD-OT-BNH2 of this invention exhibits high sensitivity to NO and a low detection limit, meeting the needs for trace NO detection. This fluorescent probe demonstrates good selectivity and anti-interference ability against common metal ions, anions, biothiols, and reactive oxygen species. After reacting with NO, both the absorption and emission spectra of the fluorescent probe undergo a significant redshift, generating a clear fluorescence signal within the NIR-II imaging window, suitable for deep tissue imaging. The fluorescent probe possesses aggregation-induced emission properties, which enhances its fluorescence imaging performance in complex biological environments. This fluorescent probe exhibits low cytotoxicity and good biocompatibility, and can be used for visual detection of nitric oxide at the cellular and in vivo levels.