121results about How to "Improve imaging depth" patented technology

The invention relates to an electronic display that combines the optical function of the display and part of the electronic function of the display into an array of individual fibers. The individual fibers contain a lens or optical function and at least one set of electrodes. Containing the lens function and the address electrode in the same fiber assures alignment of each pixel with its representative lens system and allows for the fabrication of very large three-dimensional, direct view displays. The electronic part of the displays can function as a plasma display (PDP), plasma addressed liquid crystal (PALC) display, field emission display (FED), cathode ray tube (CRT), electroluminescent (EL) display or any similar type of display.

The invention discloses a three-dimensional high-definition mammary gland imager in the biological medical imaging domain, which is characterized by the following: judging pathologic position and degree of mammary gland tissue according to optical parameter according to grey, tissue structure and size semi-quantitatively or qualitatively; adopting infrared (600-900nm) as light source; modulating space of irradiated light through coded aperture without harmful detecting; forming digital infrared optical scanning hologram; utilizing digital decoded technique to extract the information of galactophore tissue; displaying three-dimensional high-definition image.

The invention discloses an extra-large imaging depth three-dimensional display method based on an optical 4f system, belongs to the technical field of helmet-based three-dimensional display and mainly solves the problems that the three-dimensional display image has small depth and cannot be displayed in a monocular helmet in a three-dimensional mode and people are easily fatigue after watching the image for a long time. The method comprises the following steps: segmenting a three-dimensional image to be displayed into a series of two-dimensional images with different imaging depths, respectively modulating the two-dimensional images with different imaging depths by employing stripe images in different directions to acquire a coded image; putting the coded image on an input panel of the optical 4f system, putting a phase template on the spectrum surface, wherein the phase template is formed by splicing a series of lenses with different focal distances, redisplaying the coded two-dimensional images at the imaging positions of different corresponding depths on the output panel of the optical 4f system, and realizing extra-large imaging depth three-dimensional display.

The invention provides a tissue optical clearing agent and an application thereof. The tissue optical clearing agent comprises 30-70 wt% of fructose, 2-10 wt% of octyl phenoxy poly ethoxy, 5-25 wt% of N,N,N',N'-tetra (2-hydroxypropyl) ethylene diamine and the balance of water. The tissue optical clearing agent does not damage the spatial structure of collagen in tissue, a secondary harmonic signal diagram or a fluorescent signal diagram of collagen of the tissue can be obtained, and the tissue optical clearing agent can greatly increase an imaging depth.

The invention discloses a footpad skin tissue optical clearing agent, which includes fructose, ethanol, water, and at least three of the following substances: polyethylene glycol-400, sorbitol, glucose, propylene glycol, glycerol, sodium dodecyl benzene sulfonate, dimethyl sulfoxide, butylbenisothiazolene, azone, peppermint oil and petroleum jelly, wherein the mass percentage of fructose in the footpad skin tissue optical clearing agent is 20%-75%, the mass percentage of ethanol in the footpad skin tissue optical clearing agent is 0.8%-1.5%, the mass percentage of water in the footpad skin tissue optical clearing agent is 7%-30%, and the balance of other substances. The present invention acts on the footpad skin tissue, so that the refractive index in the tissue can be quickly uniform, the scattering in the tissue is reduced, the tissue becomes transparent to light, imaging depth is improved, and a new method is provided to obtain information of skin blood vessels and blood flows.

The invention discloses a super-resolution imaging system comprising a femtosecond laser, a picosecond laser, a first data acquisition card, a second data acquisition card and a spatial light modulator. The dissipation light generated by the femtosecond laser and the exciting light generated by the picosecond laser are emitted to a sample and then a fluorescence signal is generated. The second data acquisition card converts the fluorescence signal into voltage information. The voltage information acts as the fitness value of a genetic algorithm. The voltage information is calculated accordingto the genetic algorithm and a maximum voltage absolute value is obtained, and a phase diagram corresponding to the maximum voltage absolute value acts as a phase compensation diagram. The phase compensation diagram is superposed on the dissipation light to perform aberration correction on the dissipation light so that the aberration introduced in the STED imaging process can be overcome, the imaging depth and the spatial resolution of the STED super-resolution imaging system can be enhanced and thus the super-resolution imaging system can be widely applied in physical medicine and other fields.

The present invention discloses an axial super-resolution double-photon fluorescence microscopic device and method, relates to the field of laser spot scanning microscopy, and realizes double-photon axial super-resolution imaging of a sample. The method emits an ultrashort pulse laser through a femtosecond laser to achieve two-photon excitation of the fluorescent sample; and performs phase modulation to the excitation light through a spatial light modulator, sequentially scans the sample with an axial solid spot and an axial hollow spot, and performs weight difference to two scanned two-photonimages obtained to finally obtain an axial super-resolution image. Compared with other two-photon axial super-resolution imaging microscopes, the device has a simple structure and a large imaging depth, and provides a good research method for life science and nanotechnology.

The invention relates to design synthesis and application of a near-infrared two-window fluorescence probe based on Aza-BODIPY, and belongs to the field of organic fluorescence probes. A near-infraredtwo-area dyestuff (NIR-II, 1000-1700nm) has a long emission wavelength and less light scattering and tissue autofluorescence interference so as to obtain better resolution ratio and imaging depth inbioimaging. An Aza-BODIPY fluorescent dye has a longer absorption emission wavelength, a narrower peak width at half height and a larger molar extinction coefficient, and is widely applied to the fields such as photodynamic therapy. The structural formula of the near-infrared two-window fluorescence probe designed and synthesized by the invention is as shown in the figure (I). According to the probe, electron-donating group julolidine is introduced onto the classic Aza-BODIPY structure, and the molecular rigidity is improved. The probe has excellent light stability and a capacity of resistingdisturbance. The probe successfully realizes near-infrared two-window imaging during the mice imaging experiment.

System for producing a simulated 3D image from a 2D image having a fresnel lens with two planes of curvature transverse to each other so as to create a substantially convex. The fresnel lens may be flexible and positioned within a mount configured with adjustable tensioning members so as to tune the optical characteristics of the fresnel lens so as to optimise production of the simulated 3D image.

The invention discloses a microscopical hyperspectral chromatography three-dimensional imaging device, which comprises a light source unit, a three-dimensional scanning platform, a front microscopical light path unit, a beam-splitting imaging unit, a signal pretreatment unit, a signal acquisition unit and a control unit. The device integrates a hyperspectral imaging technology, a microtechnique, a fluorescence molecular tomography and a three-dimensional imaging technology. The device can obtain the two-dimensional hyperspectral chromatography images under conditions of multiple wavelengths and angles of an object and can obtain the light spectrum under wavelength corresponding to each pixel; meanwhile, based on a three-dimensional image reconstruction technology, the device can obtain the three-dimensional microscopical hyperspectral image of the object; and combining with the microtechnique, the device can obtain the micro three-dimensional hyperspectral chromatography images of the surface and internal structures of micro objects. Compared with other spectrographs, the device has the advantages of more information, higher precision, more powerful functions and higher stability. The device is suitable for biomedical test, food safety test and ecological environment monitoring.

The invention provides an optical coherence tomography method. The method comprises the following steps: transversely scanning a sample point by point by using a converged object light beam, synchronously recording an interference signal after interference between wide-field backscattered light of each scanning point on the sample and reference light through an imaging device to form a spectral interference image of each scanning point, performing fast Fourier transform on the spectral interference image of each scanning point to obtain optical field complex amplitude distribution corresponding to each scanning point, combining the optical field complex amplitude distribution corresponding to the scanning points in a scanning order into a back transmission matrix of the scattering medium,carrying out singular value decomposition on the back transmission matrix, extracting principal component signals from matrix signals, removing multiple scattering noise signals and calculating a three-dimensional tomographic image at the object focal plane according to the extracted principal component signal. According to the method, tissue deep imaging can be realized and the imaging depth andthe imaging speed are improved.

The invention discloses a multi-photon exited near-infrared II fluorescence scanning micro-imaging system. In the invention, a 1220 nm femtosecond laser in the invention is introduced into an Olympusupright scanning microscope, and focused on a sample through a near-infrared anti-reflection objective lens; a near-infrared II fluorescence signal is collected by a large-diameter fiber collimator and coupled into a near-infrared anti-reflection large-diameter optical fiber, and finally detected and converted into an electrical signal by a photomultiplier tube responded by near-infrared II; and the electrical signal is amplified by a signal amplifier and input to a high speed data acquisition card for acquisition, and processed by a computer to obtain a near-infrared II fluorescence intensityimage of the sample. The invention has the characteristics of simplicity and practicality, good stability, tunable excitation wavelength and flexible use mode.

The invention discloses a two-photon fluorescence microscopy device based on photon recombination. The device comprises a femtosecond laser device, an optical parameter oscillator, a first half wave plate, a polarization beam splitter, a second half wave plate, a first quarter wave plate, an objective lens, an array detector and a computer, wherein the femtosecond laser device is used for sending ultra-short pulse laser to perform two-photon excitation on samples; the first half wave plate is used for regulating the proportion of light s and light p of emergent light of the optical parameter oscillator; the polarization beam splitter is used for regulating the light intensity; the second half wave plate is used for regulating the polarization direction of the line polarization; the first quarter wave plate is used for converting the line polarization light into round polarization light; the objective lens is used for focusing the round polarization light into solid round spots for illuminating a sample and exciting the fluorescence; the array detector consists of a plurality of detector units, and is used for collecting fluorescence signals; the computer processes the fluorescence signals obtained by each detector unit to obtain an image corresponding to one object point. The invention also discloses a two-photon fluorescence microscopy method based on photon recombination. The detector array is used for replacing the original single needle hole detector, so that the obtained image has a higher signal-to-noise ratio.

The invention provides a super-continuum spectrum light source based multicolor fluorescence imaging system. The system comprises a super-continuum spectrum laser generating device, a laser wavelength selecting device and an imaging device which are coupled in turn, wherein the super-continuum spectrum laser generating device is used for emitting super-continuum spectrum laser beams to the laser wavelength selecting device; the laser wavelength selecting device is used for successively selecting the single-wavelength laser beams in different wavelengths from the acquired super-continuum spectrum laser beams according to a preset rule; and the imaging device is used for acquiring a plurality of monochrome fluorescent light emitted by a to-be-detected object after being motivated by a plurality of single-wavelength laser beams in turn, generating a plurality of monochrome fluorescent images according to the acquired monochrome fluorescent light and stacking the monochrome fluorescent images, thereby acquiring a multicolor fluorescent image of the to-be-detected object. The irradiated frequency and time of different fluorogens in the to-be-detected object can be reduced and photo-thermal damage and light bleaching can be effectively reduced.

The invention provides a compressed sensing multi-photon body imaging device and method and an optical system. The optical system comprises a photomultiplier, a first convergent lens, a filter, a first beam expander, a dichroscope, a second beam expander, an objective lens, a first galvanometer, a first collimating lens, a second galvanometer, a second convergent lens, a second collimating lens, athird convergent lens and a femtosecond laser. An exciting light path in the light path comprises the third convergent lens, the second collimating lens, the second convergent lens, the second galvanometer, the first collimating lens, the first galvanometer, the second beam expander, the second dichroscope and the objective lens which sequentially pass through the femtosecond laser to reach a sample; a detection light path in the light path sequentially passes through the objective lens, the second dichroscope, the first beam expander, the filter and the first convergent lens from the sampleto the photomultiplier.

The invention discloses an optical coherence tomography system with a reference arm capable of synchronously scanning three-view imaging. The system comprises an OCT core component, a sample arm and the reference arm, optical fibers of the sample end of the OCT core component are connected with a first optical fiber collimator in the sample arm, and optical fibers of the reference end of the OCT core component are connected with a second optical fiber collimator in the reference arm; a first beam scanning galvanometer, a first focusing lens, a first focal length offset wave plate and a sample are arranged in the sample arm in sequence from top to bottom. According to the optical coherence tomography system with the reference arm capable of synchronously scanning three-view imaging, the number of imaging views of the optical coherence tomography system aiming at a tubular structure is effectively increased, and imaging is conducted on the sample in upper, left and right directions, so that the imaging depth of the system is improved through an image mosaic mode, and a good technical solving scheme is provided for comprehensive clear imaging of tubular structures like blood vessels.