62 results about "Microtechnique" patented technology

The invention relates to a confocal imaging system in fields of optical microimaging, it is a confocal microscopic system which uses virtual confocal pinhole to make the system to obtain the longitudinal chromatography capability. It's widely used in the fluorescence microscopy, optical microscopy and so on which possess the microtechnique of three-dimensional imaging capability. The invention includes: light source, collimation lens, beam splitter, microscope objective, objective table, collective lens and CCD. It characterized in that the photosensitive surface of CCD is directly located at the focal plane of the collimation lens, the computer sets the virtual pinhole at the corresponding position of two-dimensional digital image collected by CCD based on the position of the focal point of collimation lens, the signal values of the pixels in the pinhole are accumulated as the signal intensity of current scanning point to eliminate the effect of stray light in non-focal plane to the image quality. The function of virtual confocal pinhole is significant with the physical confocal pinhole and the position, size can be controlled and adjusted by computer, and it possesses the advantages of convenient gauging adjustment.

The invention relates to a high-speed WFOV (wide field of view) CARS (coherent anti-stokes raman scattering) microscope system and a high-speed WFOV CARS microscope method. In the invention, pumping laser and Stokes light laser which are totally coincident in the aspects of space and time are subjected to weak convergence, so that a sample generates a CARS signal; and the CARS signal enters a CCD(Charge Coupled Device) camera through an optical filter and a cylindrical lens so as to obtain a clear CARS image. The invention utilizes the CARS signal to image and relates to an imaging technology based on the vibration characteristic of energy level inside molecules. The high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method can be used for detecting chemical compositions of the sample and can be used for carrying out imaging on a single cell, even a single organelle. The requirements of most of biological experiments are totally met. The technical problemsof low imaging speed, series photic damage to living biological tissues and the like of the existing CARS microtechnique are solved. Compared with the common fluorescence microscopy, the high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method have the advantages that an external fluorescent probe does not need to be used, and influence on the molecular structure of the sample cannot be caused.

The invention is applied to the fields of optics, biology, chemistry and the like and provides a super resolution fluorescence lifetime imaging method. The method comprises the following steps of: sparsely activating an optical switch dye molecule marked in a sample; exciting the activated optical switch dye molecule in the sample; collecting photons transmitted by the activated optical switch dye molecule and recording a fluorescent image of the optical switch dye molecule; carrying out the centroid positioning on the optical switch dye molecule in the fluorescent image; counting the photons received at the centroid positioning site and determining a fluorescence lifetime of the activated optical switch dye molecule; and constructing the super resolution fluorescence lifetime image by combining the centroid positioning result with the fluorescence lifetime of the obtained optical switch dye molecule. By combining the super resolution fluorescence microtechnique based on unimolecule positioning with the fluorescence lifetime imaging based on time relevant single photon counting, the invention realizes the super resolution fluorescence lifetime imaging, breaks through the traditional optical diffraction limit and has higher scientific significance and application value.

The invention discloses a detecting device and a detecting method for dark field cofocal subsurface based on two coaxial conical lenses. Light emitted by a point light source passes through a collimating lens to form parallel light; the parallel light sequentially passes through a beam expander, a conical lens I and a conical lens II to form ring light; the ring light is converged to a to-be-tested sample by a beam splitting prism and an objective lens; reflected light and scattered light which are emitted by the to-be-tested sample pass through the objective lens and the beam splitting prismsequentially and enter a detection complementary diaphragm; the reflected light is shielded by the detection complementary aperture; the scattered light passes through the detection complementary diaphragm, a collecting lens and a detection pinhole sequentially and enters a photoelectric detector. The ring light illumination with adjustable duty factor is realized by adopting a beam expander and one group of symmetrically-putted coaxial conical lenses; dark field cofocus is realized by in combination with the detection complementary diaphragm; the problems of low signal-noise ratio, non-adjustable duty ratio of a shield type ring optical generator and great energy loss in the detection of the subsurface by an ordinary cofocal microtechnique are solved; the detecting device and the detecting method are suitable for subsurface nondestructive testing.

The invention discloses an electrochemical atomic force microscope probe carriage-electrolytic cell device and belongs to the field of scanning probe microtechnique. Common glass is replaced with transparent conductive glass for a window of an atomic force microscope probe carriage, the transparent conductive glass is used as a counter electrode, the counter electrode is parallel to an operating electrode, the counter electrode is larger than the operating electrode in area, the problem of nonuniform electric field distribution is solved, and layout and installation of the electrodes in the electrolytic cell are simplified. Using the transparent conductive glass as the window of the atomic force microscope is nearly impervious to laser adjustment and detection of the atomic force microscope.

The invention discloses a phase conjugate reflection bi-pass lighting confocal microscopic device, belonging to an optics measuring microtechnique. A collimation beam expander and a spectroscope are configured on a laser direct light path in sequence; a focusing objective lens and a three-dimensional micrometric displacement objective table are configured on a spectroscope reflecting light path; a collecting objective lens is configured on a spectroscope transmission light path; a collecting objective lens converging light is conducted to a photoelectric detector through conduction optical fibers; an ellipsoid reflecting lens is also configured on the spectroscope reflecting light path; a near focus of the ellipsoid reflecting lens is located on a surface of a sample put on the three-dimensional micrometric displacement objective table; and a phase conjugate reflecting lens is configured at a remote focus of the ellipsoid reflecting lens. According to the device, bi-pass lighting to a large curvature convex surface is carried out by adopting a characteristic that a reflection ray as a phase conjugate light wave of an incidence light of the phase conjugate reflecting lens can go back along an original way in combination with a characteristic that the ellipsoid reflecting lens is provided with a pair of isoplanatic image formation conjugate focuses, so that the device has a measurement ability of the large curvature convex surface and a characteristic of a high axial resolution force.

The invention discloses an ellipsoid-based reflecting lighting confocal measuring device, belonging to an optics microtechnique. A collimation beam expander, a large numerical aperture focusing objective lens, a pinhole, a three-dimensional micrometric displacement objective table, a focusing objective lens, a detecting pinhole and a detector are arranged on a laser direct light path in sequence; an ellipsoid reflecting lens is configured on the laser direct light path and at a position between the focusing objective lens and the detecting pinhole; a remote focus of the ellipsoid reflecting lens is located on the pinhole; a near focus of the ellipsoid reflecting lens is located on a surface of a sample arranged on the three-dimensional micrometric displacement objective table. With the adoption of the ellipsoid-based reflecting lighting confocal measuring device, a defect that an axial resolution force in a traditional confocal measuring technology is limited by an objective lens numerical aperture is overcome, lighting to large numerical apertures is realized, so that a transverse resolution force and the axial resolution force of a system are greatly improved.

The invention provides a method for representing a nano film micro-area deformation area by virtue of combination of a photetching technique and a transmitted electron microtechnique and belongs to the field of film nanoindentation representation. The invention discloses a method for representing a 10-100 nano film microarea deformation area by virtue of combination of a photetching technique and a transmitted electron microtechnique. According to the method, an indentated film is directly transferred into a transmission electron microscope so as to be observed by virtue of a film transfer technology; the microdefect formation, interaction and evolutionary process during a nano film elastoplasticity transition process in a nanoindentation acting process is directly disclosed from the aspect of atom scale; the direct relation between microstructure and macromechanic performance is disclosed; and the method belongs to a film nanoindentation representation method.

The invention discloses a post-processing method to enhance the damage threshold of a fused quartz optical element. First, a fluorescence confocal microtechnique is employed to detect the distribution scope and scale of subsurface defects of the fused quartz optical element; and multi-frequency ultrasonic alternating multiplexing assisted chemical etching technique through KHz and MHz frequencies is used for etching different depths according to subsurface defects in different depth distribution; different frequencies are used for different scales of subsurface defects to peel off the subsurface defects layer by layer, in order to enhance the damage threshold. The post-processing method has global processing capability on optical element; hydrofluoric acid corrosion treatment completely removes a polishing deposited layer to expose the scratches on the subsurface damage layer, and sharp new look of the scratches are well passivated; the introduction of multi-frequency ultrasonic / megasonic assist can act on scratch passivation at different scales to prevent re-deposition of the etching reaction byproducts and improve the process stability. The method can stably and greatly enhance the damage threshold of the fused quartz optical element.

The invention belongs to the technical field of biological detection, and specifically relates to a compound and a method for in-situ detecting o-sulfhydryl protein in an organism. A kind of micromolecular fluorescent probes capable of specifically detecting o-sulfhydryl of protein are designed and synthesized, and a method for in-situ fluorescence labeling and detection of endogenous o-sulfhydryl protein in the organism is built by combining fluorescence polarization technology, protein fluorescent electrophoresis and confocal fluorescence microtechnique.

The invention discloses a method for detecting depth of a subsurface crack of an optical material. The method comprises the following steps: polishing one small-angle inclined plane on a sample surface subjected to abrasive machining by adopting an angle method, and corroding the sample surface and the inclined plane by utilizing a specific etching liquid, thereby guaranteeing that a surface polishing deposited layer is removed; carrying out three-dimensional scanning on a sample subjected to angle polishing by utilizing a laser confocal microscopy, and obtaining a three-dimensional chromatography structure of a crack layer of the sample; and converting the three-dimensional structure of the crack layer into a two-dimensional graph, carrying out data processing, and finally obtaining the depth of the subsurface crack of the to-be-detected material. The method disclosed by the invention overcomes the defects of an existing angle polishing method used for detecting the depth of the subsurface crack by utilizing a laser confocal microtechnique and is efficient, high in precision and portable when being used for detecting the depth of the subsurface crack of the optical material.

The invention discloses a fluorescence labeling method for free calcium ions of a cotton pollen tube, and belongs to the technical field of biology. The fluorescence labeling method comprises the following steps: perforating glass slide to make a small chamber for culturing the pollen tube; culturing pollen in the small chamber, and sprouting the pollen tube; loading Fluo-3AM into the cultured pollen tube by a low temperature incubation method; and observing dynamic variation of Ca2+ in the pollen tube under a confocal laser scanning microscopy. The invention establishes a method for researching the concentration change of the Ca2+ in the cotton pollen tube by using the confocal laser microtechnique, has simple operation and good labeling effect, plays an important role in the growth of the cotton pollen tube and fertilization reproduction research, and has important practical application value.

The invention belongs to the technical field of biochemical separation and analysis, and particularly relates to a micro-fluidic chip, a system and a method for sorting and enriching cells in cerebrospinal fluid. The chip can avoid pretreatment operations such as sample pretreatment and cell labeling and realize fast and efficient sorting and enriching functions for a small quantity of cells in the cerebrospinal fluid and further can realize in-situ counting and fast distinguishing of the small quantity of cells in the cerebrospinal fluid in combination with an in-situ microtechnique. The system integrates fast and efficient separation and enrichment of the small quantity of cells in the cerebrospinal fluid and in-situ microscopic observation, can realize sorting, enrichment, counting, observation and detection for the small quantity of cells in the cerebrospinal fluid, is high in functionality and high in efficiency, and can be used for clinical fast and efficient detection for cerebrospinal fluid cells.

The invention belongs to the technical field of micro-spectrum imaging detection, and relates to a transmittance type differential confocal CARS (Coherent Anti-Stokes Raman Scattering) micro-spectrum testing method and a device. The key point of the method is that a CARS spectrum detection technique is combined with a differential confocal microtechnique and applied to spectrum detection and geometric measurement on transparent substances, the differential confocal microtechnique is adopted to achieve precise focus fixation and geometric measurement, and after precise focus fixation, an optimal spectrum signal of a transparent sample in a focal position of an objective lens is rapidly detected by using the CARS spectrum detection technique, then spectrum imaging of high spatial resolution is achieved, and a device for geometric morphology measurement, spectrum information measurement and atlas and spectrum integration measurement on micro transparent objects is formed. According to the method, by using the CARS spectrum detection technique, CARS spectrum information with information of a tested transparent sample can be excited within a short time (dozens of milliseconds), possibility is provided for rapidly detecting components of transparent samples, and thus the method has wide application prospects in fields such as biological medicines and material detection.

The invention belongs to the technical field of micro-spectrum imaging detection and relates to a reflective differential and confocal CARS micro-spectrum testing method and device. The core thought of the invention is that dual laser devices serve as light sources to excite Rayleigh light and CARS light having spectral characteristics of a tested sample, a dichroic beam splitting system is utilized to conduct nondestructive separation on the Rayleigh light and the CARS light, geometrical detection and positioning are conducted on the Rayleigh light, and spectral detection is conducted on the CARS light. The characteristic that a differential and confocal curve zero crossing point accurately corresponds to a focal position is utilized to form the method and device capable of achieving high-spatial-resolution spectrum detection of in a sample micro-area. By combining with a CARS microtechnique, the time for exciting Raman scattering light carrying sample information is shorter than that for a traditional Raman effect, and nondestructive sample detection can be rapidly performed. The method and device have the advantages of being accurate in positioning, high in spatial resolution, capable of achieving nondestructive detection, high in spectrum detection detectivity and the like, and a new way is provided for micro-area spectrum detection and geometrical measurement.

The invention belongs to the technical field of micro-spectrum imaging detection, and relates to a reflection-type confocal CARS micro-spectrum test method and a device. According to the reflection-type confocal CARS micro-spectrum test method, fusion of laser confocal microtechnique and CARS spectrum detection technology is realized, a binary beam splitting system is adopted for nondestructive separation of Reyleigh scattering light and CARS light, wherein CARS light is used for spectrum detection, and Reyleigh scattering light is used for geometric positioning. Accurate capturing and positioning of exciting light focal point position is realized based on the corresponding performance of confocal curve top point to focal point, high precision geometric detection and high spatial resolution spectrum detection are realized, and the method and the device capable of realizing sample microdmain high spatial resolution spectrum detection are constructed. The intensity of sample information-loaded Raman scattering light excited via combination of CARS microtechnique is much higher than that of conventional spontaneous Raman light, excitation time is short, and possibility is provided for rapid detection of biological samples and chemical materials. The reflection-type confocal CARS micro-spectrum test method possesses advantages such as accurate positioning, high spatial resolution, high spectrum detection sensitivity, and controllable measuring focusing spot size; and application prospect in the fields such as biomedicine and material detection is promising.

The invention discloses a microscopical hyperspectral chromatography three-dimensional imaging device, which comprises a light source unit, a three-dimensional scanning platform, a front microscopical light path unit, a beam-splitting imaging unit, a signal pretreatment unit, a signal acquisition unit and a control unit. The device integrates a hyperspectral imaging technology, a microtechnique, a fluorescence molecular tomography and a three-dimensional imaging technology. The device can obtain the two-dimensional hyperspectral chromatography images under conditions of multiple wavelengths and angles of an object and can obtain the light spectrum under wavelength corresponding to each pixel; meanwhile, based on a three-dimensional image reconstruction technology, the device can obtain the three-dimensional microscopical hyperspectral image of the object; and combining with the microtechnique, the device can obtain the micro three-dimensional hyperspectral chromatography images of the surface and internal structures of micro objects. Compared with other spectrographs, the device has the advantages of more information, higher precision, more powerful functions and higher stability. The device is suitable for biomedical test, food safety test and ecological environment monitoring.

The invention belongs to the technical field of micro-spectrum imaging detection, and relates to a transmittance type confocal CARS (Coherent Anti-Stokes Raman Scattering) micro-spectrum testing method and a device. The key point of the method is that a laser confocal microtechnique is combined with a CARS spectrum detection technique, a bidirectional light splitting unit is added to a transmittance type confocal microstructure to implement nondestructive separation on Reyleigh scattering light and CARS light, the CRAS is adopted for spectrum detection, and the Reyleigh scattering light is adopted for geometrtic positioning. By virtue of the characteristic that the top point of a confocal curve precisely corresponds to a focus position, the position of an emitted spot focus is precisely captured and positioned, high-precision geometric detection and spectrum detection of high spatial resolution are achieved, and a method and a device for high spatial resolution spectrum detection on microcells of samples are formed. By using the CARS spectrum detection technique, excited Raman scattering light with information of a transparent sample is far stronger than conventional excited Raman light and is short in excitation time, so that possibility is provided for rapidly detecting biological samples and transparent materials. The method is accurate in positioning, high in spatial resolution, high in spectrum detection sensitivity, controllable in focus spot size measurement and wide in application prospect in fields such as biological medicines and transparent material detection.

The invention provides a fluorescent probe HM, and a preparation method and application thereof. The fluorescent probe is 7-(diethylamino)-3-[3-(2-hydroxy-5-methoxyphenyl)-3-oxo-1-propenyl]coumarin. The preparation method comprises the following steps: dissolving 7-(diethylamino)coumarin-3-formaldehyde and 1-(2-hydroxy-5-methoxyphenyl)ethanone in acetonitrile according to an equal molar ratio, dropwise adding piperidine, carrying out heating reflux for 20 hours, cooling to room temperature, carrying out reduced pressure suction filtration, collecting filter residues, and carrying out vacuum drying to obtain a product which is a red solid. The fluorescent probe is enhanced in detection of ClO<->, shows high sensitivity and good selectivity and stability, and has the advantages of simple and rapid detection process, accurate detection result and the like. In addition, by combining a laser confocal scanning microtechnique and a biological living body imaging technique, the novel fluorescent probe is successfully applied to detection of ClO<-> in cells and liver cancer mouse models.

The invention provides a coupling permeation efficiency optimization system of a near field scanning optical microscope (NSOM) and an optimization method thereof, relating to the field of scanning probe microtechnique. The optimization system comprises a computer, a detector, a collection mirror, a probe, a focusing mirror, a spatial light modulation instrument and a driver, wherein the computer,the detector, the spatial light modulation instrument and the driver form a closed loop circuit. In the system, the spatial light modulation instrument is introduced to modulate the light field distribution of an incident light field, so that the modulated light field is coupled with the probe, thereby having highest coupling permeation efficiency, and being incapable of reducing other performances of a microscope system.

The invention discloses an improved scanning method for a scanning probe microscope. In the method, in a forward scanning process which requires signal collection by the scanning probe microscope, interacting information of a probe to a sample is collected, and in a return scanning process which does not require signal collection, an improved return blank scanning control method is employed so that interaction between the probe and the sample is weakened or the probe and the sample are completely separated from each other. In the return scanning process, which does not require signal collection, of the scanning probe microscope, the interaction between the probe and the sample is weakened or the probe and the sample are completely separated from each other, so that invalid abrasion or consumption is effectively reduced or eliminated during the return scanning process. The method increases service life of a probe and also reduces use cost of a microscopy. The method can be widely used in the technical field of microscopy.

The invention discloses an optical-fiber endoscopic coal combustion ultralow emission fly ash concentration measuring device, which is characterized by comprises an image acquisition part, an image processing part and an anti-dust device. The image acquisition part comprises a combined shooting unit (1), a transmission fiber optic bundle (3), a metal sleeve (4), an endoscope ocular lens (6), a coupling part (7) and a high-speed camera (8). The transmission fiber optic bundle (3) is arranged in the metal sleeve (4) with a hollow structure, and is respectively connected to the combined shooting unit (1) and the endoscope ocular lens (6). The endoscope ocular lens (6) is connected to the high-speed camera (8) through the coupling part (7). The image processing part comprises an optical cable (9), an acquisition card (10) and a computer (11). By integrating a high-speed shooting technology, an optical-fiber endoscopic technology, a microtechnique and an image processing technology, on-line measurement of concentration of ultralow-concentration fine particles in a stationary pollution source exhaust gas from a coal-fired power plant can be realized.

An electrically reversible molecular electronic device based on scanning tunnel microtechnique is composed of substrate with an electrically conductive surface having molecular-class smoothness, an organic molecular layer which is a vertically orientated single molecular layer, and the probe which is the needle tip in scanning tunnel microscope. At the action of positive and negative pulses, its states can be reverisibly switched.

The invention discloses a UV (Ultraviolet) straight printing method of a 3D (Three-Dimensional) picture and relates to a 3D picture printing method. The printing method comprises the following steps: cutting grating material billet into a corresponding size according to the size of the picture to obtain a to-be-printed grating material plate; preparing a UV ink printing device; accurately measuring the size on the bearing platform of a flat straight printer; printing aligning indicating lines of the to-be-printed grating material plate in both longitudinal and transverse directions of the bearing platform; placing the cut grating material plate on the bearing platform of the flat straight printer and roughly aligning the grating material plate with the indicating lines; displaying the grating material plate amplified by an optic microscope by virtue of a computer microtechnique; carrying out accurate visual fine-tuning aligning according to the aligning indicating lines, so as to ensure the grating material plate is completely overlapped with the aligning indicating lines; configuring a print starting position on the computer according to the position of the grating material plate on the bearing platform; starting print procedures and obtaining a 3D picture UV straight print production when the printing is finished.

The invention discloses an ultrahigh-resolution non-linear optical microscopy system, and relates to the field of laser detection. According to the system, a non-linear optical technique and a stimulated emission depletion microtechnique are combined, a beam of short pulse infrared laser is used as a multi-photon stimulated light source, another beam of super-short laser pulse of a wide tuning range, which is generated from a synchronous short pulse laser pumping frequency doubling pumping light parameter oscillator, is expanded to be 200ps in pulse width by virtue of a single-mode optical fiber; as a stimulated emission depletion light source, the stimulated light is used for stimulating fluorescent molecules; the fluorescence at the periphery of a stimulated light spot focus is quenched by virtue of the stimulated emission depletion light, and then the non-linear optical microscopy system can break through the light wave diffraction limit. According to the system, in the non-linear optics, infrared laser with long wavelength is used as the multi-photon stimulated light source with high penetration depth because of small scattering influence, and ultrahigh resolution tomography imaging of a living organism tissue is facilitated.

The invention relates to a compound cantilever beam needle point used for a micro-nano microtechnique and a manufacturing method thereof. The compound cantilever beam needle point comprises a base, a cantilever beam and a needle point and is characterized in that the base, the cantilever beam and the needle point are respectively provided with an insulating dielectric layer which is distributed with four pressure-sensitive resistors with same resistance value, wherein two pressure-sensitive resistors are positioned on the base, and the other two pressure-sensitive resistors are positioned on the cantilever beam and distributed uniformly with the direction being parallel to the length direction of the cantilever beam; and the four pressure-sensitive resistors are connected into a Wheatstone bridge by wires. The compound cantilever beam needle point does not use laser positioning to measure small strain generated by the cantilever beam needle point of a scanning probe microscope, can couple the pressure-sensitive resistors capable of measuring the small strain accurately with the cantilever beam needle point so as to measure dynamical signals when the cantilever beam and a sample act, as well as information of magnitude of electrostatic force and distribution of magnetic field and the like. The invention belongs to the technical field of micro-mechanical sensors.

The invention discloses an ultrahigh-resolution nonlinear fluorescence excitation microscopic system based on Bragg diffraction crystals, and relates to the field of laser detection. The system is combined with a nonlinear optical technology and a stimulated emission depletion microtechnique, infrared lasers with long wave lengths are adopted as a multiphoton excitation light source, a continuous laser beam is adopted as a depletion light source, the excitation light source is used for exciting fluorescent molecules, stimulated emission depletion light quenches fluorescence on the periphery of an excitation light spot focus, and the nonlinear optical microscopic system can break through the light wave diffraction limit. The Bragg diffraction crystals are introduced, the repetition frequency of femtosecond lasers is reduced below 4 MHz, and the fluorescence quantum yield of the fluorescent molecules is obviously improved. Meanwhile, the continuous stimulated emission depletion light is modulated into synchronization pulse lasers with the repetition frequency equal to that of the femtosecond lasers by the Bragg diffraction crystals, and on the basis of simplifying the device, the energy of incident light is reduced, and photobleaching on samples is reduced.

The invention provides an aluminum alloy surface wire drawing quality detecting and evaluating method. The confocal microtechnique is utilized, data collection is conducted on determined detecting points on a work test piece, the three-dimensional data is calculated and evaluated according to different formulas, and accordingly the wire drawing quality is determined. The aluminum alloy surface wire drawing quality detecting and evaluating method is high in image resolution and detail resolution, and the evaluating algorithm is simple and effective.

The invention discloses an intelligent cell identification method based on the laser confocal microscopy technology. The method comprises the following steps: (1) carrying out culture and fluorescentstaining treatment on cells; (2) adopting a laser confocal microscope to obtain a layer-cut image of a cell multi-fluorescence channel; (3) sequentially carrying out graying, denoising processing andthreshold segmentation on the layer-cut image, picking out a cell region, and storing gray data of the same fluorescence channel into a three-dimensional matrix; (4) determining each cell region and labeling by adopting a region random growth model; (5) sequentially carrying out interpolation three-dimensional reconstruction on the labeled cells, and calculating three-dimensional morphological parameters of each cell; (6) repeating the processes (1) to (5) by using different cells, and learning the three-dimensional morphological characteristics of the cells by using a machine learning methodto finally realize the identification of various cells. According to the identification method provided by the invention, various cells can be rapidly and effectively identified on the basis of the three-dimensional morphological characteristics of the cells obtained by the laser confocal microscopy technology.

A symmetrical out-focus type axial high-resolution confocal microimaging device belongs to the fields of optical accurate measurement. The symmetrical out-focus type axial high-resolution confocal microimaging device comprises a lighting light path and a detecting light path. The lighting light path is that a light beam emitted by a laser can be aligned by an alignment lens set to parallelly penetrate through a polarizing beam splitter and a 1 / 4 lambda water plate to be converged by a focus microscope objective on an article to be tested. The detecting light path is that the light beam reflected by the article to be tested passes through the focus microscope objective and the 1 / 4 lambda wave plate to be reflected by the polarized beam splitter on a 1:1 ordinary beam splitter. A reflecting light path and an incident light path of the 1:1 ordinary beam splitter are both provided with detecting light paths to achieve detection of two light beams. The symmetrical out-focus type axial high-resolution confocal microimaging device is equal to a differential confocal microtechnique. Furthermore, the out-focus amount can be set accurately to enable the systematical axial resolution capability to be optimal, and meanwhile, complex pinhole symmetrical out-focus adjustment is removed.