Reflective differential and confocal CARS micro-spectrum testing method and device

A differential confocal and spectral testing technology, applied in Raman scattering, material excitation analysis, etc., can solve problems such as restricting applications and limiting the ability of the CARS microscopic system to detect micro-region spectra, so as to improve the lateral resolution of the system, Improve the signal-to-noise ratio and improve the effect of spectral signal-to-noise ratio

Pending Publication Date: 2017-08-11
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

[0007] The above reasons limit the ability of the CARS microscope system to detect micro-region s

Method used

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  • Reflective differential and confocal CARS micro-spectrum testing method and device
  • Reflective differential and confocal CARS micro-spectrum testing method and device
  • Reflective differential and confocal CARS micro-spectrum testing method and device

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Embodiment

[0055] In this embodiment, a picosecond laser with a wavelength of 532nm is used as the pump light source and a probe light source, and a supercontinuum picosecond laser with the same repetition rate is used as the Stokes light source with a bandpass filter of 550-650nm , mixed and emitted under the conditions of spatial coincidence and time consistency, the structured beam is obtained through the pupil filter and then tightly focused on the sample through the high-power microscope objective lens. At this time, the phase matching condition is met, and the excitation wavelength range is 450-515nm. Anti-Stokes light (CARS) and Rayleigh light with a wavelength of 532nm.

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Abstract

The invention belongs to the technical field of micro-spectrum imaging detection and relates to a reflective differential and confocal CARS micro-spectrum testing method and device. The core thought of the invention is that dual laser devices serve as light sources to excite Rayleigh light and CARS light having spectral characteristics of a tested sample, a dichroic beam splitting system is utilized to conduct nondestructive separation on the Rayleigh light and the CARS light, geometrical detection and positioning are conducted on the Rayleigh light, and spectral detection is conducted on the CARS light. The characteristic that a differential and confocal curve zero crossing point accurately corresponds to a focal position is utilized to form the method and device capable of achieving high-spatial-resolution spectrum detection of in a sample micro-area. By combining with a CARS microtechnique, the time for exciting Raman scattering light carrying sample information is shorter than that for a traditional Raman effect, and nondestructive sample detection can be rapidly performed. The method and device have the advantages of being accurate in positioning, high in spatial resolution, capable of achieving nondestructive detection, high in spectrum detection detectivity and the like, and a new way is provided for micro-area spectrum detection and geometrical measurement.

Description

technical field [0001] The invention belongs to the technical field of microspectral imaging, and relates to a reflective differential confocal CARS microspectral testing method and device, which can be used to quickly detect micro-area anti-Stokes scattering (CARS) spectra of various samples, and can Realize high spatial resolution imaging and detection. [0002] technical background [0003] Optical microscopes are widely used in the fields of biomedicine and material science. With the rapid development of modern science, the requirements for microscopic imaging have shifted from structural imaging to functional imaging. In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographic imaging characteristics of confocal microscopy, and has the ability of ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 赵维谦黄伟华邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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