Reflective differential and confocal CARS micro-spectrum testing method and device

A differential confocal and spectral testing technology, applied in Raman scattering, material excitation analysis, etc., can solve problems such as restricting applications and limiting the ability of the CARS microscopic system to detect micro-region spectra, so as to improve the lateral resolution of the system, Improve the signal-to-noise ratio and improve the effect of spectral signal-to-noise ratio
CN107037031APending Publication Date: 2017-08-11BEIJING INSTITUTE OF TECHNOLOGYGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING INSTITUTE OF TECHNOLOGYGY
Publication Date
2017-08-11

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Abstract

The invention belongs to the technical field of micro-spectrum imaging detection and relates to a reflective differential and confocal CARS micro-spectrum testing method and device. The core thought of the invention is that dual laser devices serve as light sources to excite Rayleigh light and CARS light having spectral characteristics of a tested sample, a dichroic beam splitting system is utilized to conduct nondestructive separation on the Rayleigh light and the CARS light, geometrical detection and positioning are conducted on the Rayleigh light, and spectral detection is conducted on the CARS light. The characteristic that a differential and confocal curve zero crossing point accurately corresponds to a focal position is utilized to form the method and device capable of achieving high-spatial-resolution spectrum detection of in a sample micro-area. By combining with a CARS microtechnique, the time for exciting Raman scattering light carrying sample information is shorter than that for a traditional Raman effect, and nondestructive sample detection can be rapidly performed. The method and device have the advantages of being accurate in positioning, high in spatial resolution, capable of achieving nondestructive detection, high in spectrum detection detectivity and the like, and a new way is provided for micro-area spectrum detection and geometrical measurement.
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Description

technical field

[0001] The invention belongs to the technical field of microspectral imaging, and relates to a reflective differential confocal CARS microspectral testing method and device, which can be used to quickly detect micro-area anti-Stokes scattering (CARS) spectra of various samples, and can Realize high spatial resolution imaging and detection.

[0002] technical background

[0003] Optical microscopes are widely used in the fields of biomedicine and material science. With the rapid development of modern science, the requirements for microscopic imaging have shifted from structural imaging to functional imaging. In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographic imaging characteristics of confocal microscopy, and has the ability of ...

Claims

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