The invention belongs to the field of
hypertension treatment drug related gene detection, and particularly relates to a high-
throughput sequencing method for
hypertension treatment drug related genesand an application of the high-
throughput sequencing method. The invention discloses the high-
throughput sequencing method for
hypertension treatment drug related genes and the application of the high-throughput sequencing method. The method comprises the following steps: determining hypertension treatment drug related genes, designing a hypertension treatment drug
related gene amplification primer combination, extracting
genome DNA and detecting concentration, performing multiple a PCR amplification reaction and product purification on the hypertension treatment drug
related gene, performingjoint sequence PCR reaction and product purification, performing
library quality inspection, performing high-throughput sequencing and the like. The method can realize
typing detection of 39 SNP lociof 33 hypertension treatment drug related genes at one time, 27 common hypertension treatment medicines are covered, more detection sites, higher flux, higher
data accuracy and higher economy are realized, guidance can be provided for individualized medication of patients, the
medicine treatment efficiency is improved, and the risk of toxic and side effects is reduced.