245 results about "Confocal microscopy" patented technology

The invention discloses a quality detection method and system for vehicle safety belt production and a storage medium, and relates to the field of manufacturing quality control of automobile passive safety parts. The method aims at solving the problems that an existing detection means is weak in tiny defect recognition capacity, insufficient in defect evolution modeling and lack of intelligent feedback regulation and control. According to the method, multiple detection means such as medium-wave infrared thermal imaging, laser confocal microscopy, structured light three-dimensional reconstruction and capacitance sensing are fused, and multi-mode defect sensing of key parts such as a braid, an insertion area and an adhesive layer is achieved; deducing a defect evolution process and generating a high-credibility pseudo label by constructing a thermal-stress-rheology coupling finite element model; the graph neural network and the AI model are combined to complete defect type identification, risk quantification and grading, and automatic optimization of process parameters and production line control response are realized based on the quality grade, so that the detection precision, the response efficiency and the product yield are remarkably improved.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention discloses a tomato LACS2 gene promoter and application thereof, and belongs to the technical field of biology. On one hand, the invention provides the tomato LACS2 gene promoter, and on the other hand, the invention provides application of the tomato LACS2 gene promoter in promoting target gene expression in plants. According to the invention, a tomato LACS2 gene promoter fragment (788bp) is constructed to drive an EGFP-GUS (Enhanced Green Fluorescent Protein-Glucuronidase) gene fusion expression vector. GUS histochemical staining and laser confocal microscopy observation prove that the promoter fragment can guide normal expression of the reporter gene in the tomato epidermis. Therefore, the LACS2 promoter can be used for researching development, quality improvement and postharvest preservation of tomato peels and specific expression of exogenous genes in the tomato peels.

The invention relates to a microscope system, in particular to a confocal microscope system of a short-wave infrared band. The system comprises a bright / dark field microscopy subsystem, a photon emission microscopy subsystem, a short wave infrared laser scanning confocal microscopy subsystem, a fluorescence confocal microscopy subsystem and a control module. According to the system provided by the invention, the short-wave infrared fluorescence lifetime curve can be obtained through the electro-luminescence emission and / or photoluminescence emission of the semiconductor device in the integrated circuit, so that the defect type of the semiconductor can be judged. Besides, the system adopts a multi-subsystem optical path multiplexing structure in structural design, short-wave infrared fluorescence generated by exciting a defect or a failure point is collected while observation is facilitated, high integration level is achieved, and observation, positioning and failure analysis of the defect or the failure point can be achieved only by using a single system provided by the invention.

The present disclosure describes a turntable parallel scanning confocal microscope illumination light path, the illumination light path comprising a sleeve assembly having a light source and a field diaphragm, a lens barrel assembly having a collector lens, a beam splitting chamber having a turntable, a tube lens and a microscope objective, the light source being configured to form a first parallel beam entering the lens barrel assembly via the field diaphragm; the light source is imaged on the focal plane of the light collecting mirror; the turntable is configured to receive a second parallel light beam emitted from the tube lens, the second parallel light beam is configured to form a preset image on the turntable, the turntable is configured to receive a first parallel light beam emitted into the light splitting chamber through the lens barrel assembly, and the first parallel light beam is configured to form a light spot covering a small hole surface of the turntable on the turntable; the microscope objective is configured to be installed at the position of an image of the light source which is formed by the light beam emitted from the tube mirror and meets the preset requirement. According to the invention, the illumination light path of the turntable parallel scanning confocal microscope, which is simple and convenient to operate and can be conveniently and accurately assembled and adjusted, can be provided.

The invention relates to the technical field of medical image processing, and discloses an adversarial generative network model for three-dimensional reconstruction of a brain cell-level vascular network. The model comprises a three-dimensional blood vessel feature extraction module, a dynamic attention generator, a multi-scale discriminator and the like. Characteristics are obtained through multi-modal medical image data (magnetic resonance angiography and confocal microscope scanning data), and high-precision vascular network three-dimensional reconstruction is realized by utilizing cooperative work of all the modules. The dynamic attention generator calculates the topological connection probability of the vascular branches, and the multi-scale discriminator globally and locally evaluates the reconstruction result. Meanwhile, model training is optimized through an adversarial training controller, and the blood vessel network is perfected through a capillary network completion module. According to the method, the advantages of multi-modal data are effectively fused, the reconstruction precision is improved, and powerful support is provided for research and diagnosis of brain vascular diseases.

The invention discloses a method for segmenting different strata series of stratified shale and identifying oiliness, and belongs to the field of oil-gas exploration. The invention discloses a strata division method based on XRF (X-Ray Fluorescence) mineral scanning and single polarization-fluorescence-laser confocal optical lens combined observation, which realizes accurate identification and segmentation of micron-sized strata boundaries of a layered rock core sample from different scales, and performs group component extraction on segmented independent strata. And carrying out chromatography-mass spectrometry analysis and FT-ICR MS analysis on the extracted saturated, aromatic, non-aromatic, leached and other family components. The TSF spectrum, light and heavy components, biomarker compounds, non-hydrocarbon compounds and other data obtained through the experiments are comprehensively analyzed, layering scale oiliness difference quantification and oil source comparison are achieved, and the shale oil micro-migration phenomenon is determined. The influence of the heterogeneity of the layered rock core sample on a result obtained by a traditional analysis method is overcome. According to the technology, direct evidences are provided for accurate prediction of shale oil desserts, exploration risks are reduced, and economic benefits are improved.

The invention relates to the technical field of fluorescence immunoassay, and discloses a quantum resonance fluorescence immunoassay medical detection method, which comprises the following steps: 1, carrying out fixation and permeation treatment on cells, respectively incubating the cells by using labeled different quantum dot-antibody compounds, specifically binding antibodies with corresponding target proteins in the cells, and detecting the target proteins in the cells; a labeled cell sample is obtained; 2, under a confocal microscope, laser with a specific wavelength is adopted to excite the marked cell sample, and fluorescence lifetime image data of different quantum dot emission wavelengths are collected; according to the method, the fluorescence lifetime image data of different quantum dot emission wavelengths are acquired through the confocal microscope, the position and content information of different target proteins can be clearly distinguished, simultaneous analysis and accurate positioning of multiple target proteins are realized, and an efficient means is provided for multi-protein research in a complex biological system.

This disclosure provides microscope-based systems and methods that significantly improve image contrast and have better vertical / horizontal resolution. This disclosure uses confocal microscopy in replacement of conventional fluorescent microscopy, for example, adding a spinning disk or a confocal microscopy scanning unit on the prior invention. Using a spinning disk vastly improves the speed of image acquisition (allowing for imaging of fast dynamic processes and live specimens), and considerably reduces photo damage. Disclosed herein are some embodiments to demonstrate how to set up the spinning disk microscopy system for image-guided illumination and photolabeling.

The invention provides a confocal microscope objective lens with high optical flux and long working distance, the confocal microscope objective lens comprises a first lens group and a second lens group which are sequentially arranged along the light transmission direction, the first lens group comprises a first lens, a second lens, a third lens and a fourth lens which are sequentially arranged along the light transmission direction, the first lens is a negative lens, and the second lens is a positive lens. The working distance is prolonged; the combination of the second lens, the third lens and the fourth lens has positive focal power and is used for focusing light, so that the objective lens obtains a larger numerical aperture; the second lens group comprises a sixth lens and a seventh lens which are sequentially arranged in the light transmission direction, and the combination of the sixth lens and the seventh lens has negative focal power and is used for enlarging the aperture of the light beam and reducing the angle of the incident light beam at the same time. According to the scheme, a view field of 12 mm is achieved, the numerical aperture reaches 0.5, the working distance reaches 17.7 mm, and excellent imaging performance is achieved while the large view field, the high resolution and the long working distance are considered.

The invention relates to a spectrum detection module and a confocal microscope. The spectrum detection module comprises a dispersion element and a first spectrum splitting device located on a light emitting surface of the dispersion element, the first spectrum splitting device specifically comprises a first imaging lens group, a first slit assembly, a first converging lens group and a first photoelectric detector which are sequentially arranged along a first optical axis from the light emitting surface of the dispersion element; the first slit assembly comprises a pair of first slit halves arranged at intervals in the direction perpendicular to the first optical axis and a first driving piece in driving connection with a first slit plate, and the first slit plate is used for moving in the arrangement direction under the action of the first driving piece. A first variable slit with variable width and / or position is formed on the exit pupil plane of the first imaging lens group, the entrance pupil plane of the first converging lens group coincides with the first variable slit, the first photoelectric detector is located on the exit pupil plane of the first converging lens group, and it can be understood that the arrangement direction is the same as the spectrum expansion direction.

The invention discloses a method for characterizing the degree of fusion of new and old asphalt based on fluorescence microscopy, and the process is as follows: using a confocal microscope to observe the surface of a wood simulated aggregate coated with homogenous SBS modified asphalt, obtaining fluorescence images at different depths, calculating an average fluorescence intensity curve, and fitting a fluorescence intensity attenuation correction function; observing the mixed asphalt on the surface of the wood simulated aggregate under the confocal microscope; scanning from the outermost layer to the innermost layer of the wood simulated aggregate, and outputting a plurality of fluorescence images of the new and old asphalt in the asphalt film thickness layer; performing noise reduction processing on the fluorescence image, obtaining a concentration change curve of the new asphalt and correcting it using an attenuation correction function; calculating a concentration attenuation change curve of the new asphalt, judging the influence of noise and the asphalt fusion layer, retaining the layer position with a fitting coefficient less than or equal to 95%, and calculating the concentration attenuation change curve after removing the influence of noise and fluorescence signal attenuation; and defining the degree of fusion of the mixed asphalt by an area method.

Low-cost, portable in-vivo confocal ophthalmoscopes (PICOs) and associated methods are described that are capable of producing microscopic images of the anterior segment of the eye and the external eye structures enabling visualization of cellular and some sub-cellular structures. Some of the features of the described ophthalmoscopes include the use of a non-contact objective lens in conjunction with a grating-based, scan-less confocal microscopy approach, integration of the capability of imaging the external eye and anterior segment en face, integration of a display as part of the microscope, increased signal level and imaging speed, reduction of detected specular reflection, and attachment methods onto a slit lamp biomicroscope or for hand-held use.

The invention relates to the technical field of quantum sensing and optical instruments, and discloses an integrated scanning confocal microscope for diamond NV color center quantum sensing, which comprises a control module, an integrated circuit module, an integrated laser module and an integrated light path module, the control module is used for exciting a diamond NV color center, the integrated light path module realizes two-dimensional scanning of a laser beam on a sample focal plane through a galvanometer, an objective lens is used for collecting NV color center fluorescence, meanwhile, a single-photon counter and a camera are arranged, confocal detection and wide-field microscopic imaging are realized, and the integrated circuit module receives an instruction of the control module. The microscope can realize confocal scanning fluorescence imaging, optical detection magnetic resonance spectrum measurement and NV color center spin coherent control experiments, such as Rabian oscillation, Rahm, Haln echo and spin relaxation experiments, and provides comprehensive experimental ability for diamond NV color center quantum sensing.

The invention provides a single-photon anti-bunching measurement method and system based on an ADC acquisition card. The single-photon anti-bunching measurement method and system are mainly used for acquiring and analyzing a second-order correlation function of a single-photon optical signal. Compared with a traditional single photon detection method, the high-speed ADC acquisition card in an intermediate trigger mode is adopted, redundant data are reduced, and meanwhile the data acquisition efficiency is improved. The system comprises a data acquisition unit, a data reading module, a data processing center and an algorithm execution module, and the data acquisition unit acquires a single photon signal through a confocal microscope system and converts an optical signal into an electric signal by using an SPAD detector. The ADC acquisition card adopts an intermediate trigger mode, so that sampling can be accurately triggered in a set effective sampling interval, and a large amount of redundant data existing in a traditional sampling mode is avoided; and the completeness of the time sequence is better ensured while the data is accurately acquired.

The invention discloses an online optical clear solution for three-dimensional structure imaging of a large-volume biological sample, which is characterized in that the solution comprises iohexol, urea, 1, 2-propylene glycol, dimethyl sulfoxide, 3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid inner salt and water. The invention further discloses a three-dimensional imaging method for the large-volume biological sample. The method comprises the following steps: carrying out transparentizing treatment on the biological sample by using the online optical transparentizing solution, and carrying out three-dimensional imaging on the transparentized biological sample by using a fluorescence microscope. The fluorescence microscope comprises a confocal microscope and / or a light sheet microscope. When the online optical transparent solution is used for imaging, the transparent depth of a surface sample is large, the single imaging depth can be increased, and the cutting thickness can be improved, so that the cutting frequency is reduced, and finally the data acquisition efficiency of a large-volume sample is improved.

The invention discloses a deep learning feature-based automatic identification method for a firing pin trace, which is used for realizing accurate and automatic identification of the firing pin trace. The method comprises the following steps of: acquiring firing pin trace sample data by using a three-dimensional confocal microscope; filtering the acquired firing pin trace sample by using a spline filter to obtain firing pin trace surface roughness texture features; in consideration of an initial phase between the samples, keeping the reference sample unchanged, and rotating the to-be-detected sample in a range of [-180 degrees, 180 degrees] by taking 3 degrees as a step length; the reference sample and all the to-be-tested samples obtained through rotation are subjected to matching through a Detector-Free Local Feature Matching with Transformers (LoFTR) algorithm, and a combination with the largest number of matched feature points is selected as a preliminary matching result; in order to further improve the matching precision, the Locality Preserving Matching (LPM) technology is introduced on the basis to carry out wrong matching filtering on the preliminary matching result, so that a more reliable matching result is obtained. According to the automatic pin firing trace recognition method based on the deep learning features, whether two bullet shells are from the same gun or not can be judged according to the number of the matched feature points, and then auxiliary evidence is provided for detection of gun-related cases.

This application relates to the technical field of confocal microscopy measurement, and provides a dark-field confocal microscopy measurement apparatus and method based on multi-fractional angular momentum demodulation. The apparatus includes a modulated illumination module and a signal collection and demodulation module. The modulated illumination module obtains vortex light with different fractional orders through modulation using vortex phase patterns with different fractional orders, so as to scan a to-be-measured sample. The vortex light with different fractional orders irradiates the to-be-measured sample and is reflected out. The signal collection and demodulation module collects the reflected light and generates dark-field images, and finally performs cross-correlation processing on the dark-field images generated under the vortex light with different fractional orders, to obtain high SNR data.

The invention relates to the technical field of crystal detection, in particular to a comprehensive crystal surface crack analysis method and system based on multiple characterization technologies, and the method comprises the following steps: obtaining a three-dimensional morphology graph of a crystal surface crack by using a laser confocal microscopy technology, and measuring crystal surface stress by using a Raman spectrum technology; a curvature tracking and edge detection algorithm is adopted to extract a crack main path, a Bezier curve is combined to fit the geometric morphology of the crack, the curvature, the trend and the branch characteristics of the crack are calculated, and geometric modeling of the crack path is completed; predicting a crack initiation area by using a graph structure neural network, and dividing a crystal surface risk area; predicting a crack expansion trend by combining a phase field evolution model and a machine learning method; and fusing the risk area and the expansion trend to generate a crack risk joint heat map. According to the method, the crack initiation area and the expansion trend can be accurately identified, and a scientific basis is provided for crystal cutting process optimization and crack control.

The invention relates to a resolution improving device and method based on three-camera differential confocal, the device comprises a light source, a light path intermediate, an objective lens, an objective table, an imaging module and an industrial personal computer, the imaging module comprises a first camera placed before focus, a second camera placed on a focal plane and a third camera placed after focus; the first camera is arranged at a specified distance d in front of the focus through the first adapter ring, the second camera is arranged at the focal plane through the second adapter ring, and the third camera is arranged at a specified distance d behind the focus; light emitted by the light source is projected to a to-be-tested sample on the objective table through the light path intermediate and the objective lens, and light signals reflected on the to-be-tested sample are respectively transmitted to the first camera, the second camera and the third camera through three light paths; the industrial personal computer can control the objective lens and the objective table to move at least in the Z-axis direction. The acquisition precision of the PSF is improved by improving the positioning precision in the Z-axis direction, so that the resolution of the image acquired in the deconvolution process is improved, and the restoration effect of the three-dimensional microscopic image is improved.

Methods and apparatus for obtaining 3D imaging of tissue involve scanning a focused laser beam in xz planes to obtain a set of xz plane images spaced apart in a y direction. The xz plane images are processed to correct distortions and motions and combined to provide 3D image data. Surface flatting is optionally performed. Imaging may be performed using a femtosecond (fs) laser beam. Different components of light returning from the tissue may be detected and processed to yield plural co-registered images using different imaging modalities, for example, reflective confocal microscopy (RCM), two photon fluorescence (TPF) and second harmonic generation (SHG).

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

This invention belongs to the field of fluorescence microscopy imaging technology, specifically a calculation method for high-density fluorescence fluctuation super-resolution microscopy imaging. This method dynamically controls the activation-quenching cycle of reversible fluorescent molecules through alternating sequential irradiation with activation and excitation light, effectively reducing the density of fluorescent molecules by combining activation light control and image differential processing techniques. Specifically, it includes: optimizing light control parameters such as frame rate, activation light power and exposure time, and excitation light power and exposure time; using an inter-frame difference algorithm to reduce the effective fluorescence density; and employing the SOFI algorithm for high-order cumulant calculation to achieve super-resolution reconstruction. Compared to existing technologies, this invention eliminates the need for complex multicolor labeling, significantly improves imaging resolution through a light control-differential synergistic strategy, and is compatible with various microscopy systems such as wide-field and confocal microscopy, providing an efficient and reliable technical solution for live-cell super-resolution observation.

This invention discloses a detection method, system, and medium based on line spectral confocalization. The method includes: determining the number of peaks; if the number of peaks is one, adjusting the light intensity value corresponding to the peak wavelength to be within the light intensity working range; if the number of peaks is not less than two, extracting the light intensity value corresponding to the peak wavelength, analyzing the peak morphology, and identifying the peak wavelength associated with each reflection region; analyzing the offset of the height of the specular reflection region, correcting the detection height of the specular reflection region to obtain the height difference between the reflection regions covered within the width of the line spot at any position on the scanning line; and adjusting the distance of the sensor relative to the surface of the object under test according to the height difference, so that there is one and only one valid peak in the spectral image at any position on the scanning line. This invention can accurately calculate the height difference between the specular reflection region and the diffuse reflection region covered within the width of the line spot, improving the signal-to-noise ratio of the spectral image and expanding the application scenarios.

The invention belongs to the technical field of in-situ observation sample preparation, and particularly relates to a method for in-situ observation of a Ti alloy sample through a high-temperature laser confocal microscopy, and the method comprises the following steps: S1, grinding, polishing and ultrasonic cleaning treatment of Ti alloy; s2, performing plasma spraying treatment on the alumina crucible, and spraying a layer of yttrium oxide powder on the surface of the alumina crucible; s3, hanging a pure titanium sheet in a hearth of the high-temperature laser confocal microscope; and S4, the Ti alloy sample pretreated in the step S1 is put into a crucible, and in-situ observation is conducted through a high-temperature laser confocal microscope. The method is simple to operate, solves the problems that the Ti alloy is easy to oxidize at high temperature and is not provided with an adaptive observation crucible, and can be used for observing the microstructure evolution of the Ti alloy in situ and observing the microstructure evolution process of the Ti alloy in real time under the high-temperature laser confocal microscope so as to facilitate the subsequent research on growth mechanism analysis and growth kinetics quantification.

The present invention provides a method for constructing the light field distribution of the exit pupil plane of a confocal microscope system. By using geometric optics and reasonable mathematical approximation to analyze the information of the confocal microscope system parameters facing any two-dimensional tilt angle, the light field distribution of the exit pupil plane of the objective lens of the confocal microscope system is constructed, which not only avoids the complexity of the construction method based on diffraction theory and the huge amount of calculation when simulating the distribution, but also avoids the large error caused by approximating the reflected light as a circle in the construction method based on geometric optics, which cannot truly reflect the actual distribution. The method for constructing the light field distribution of the exit pupil plane of the confocal microscope system provided by the present invention takes into account the physical phenomena under actual conditions, and constructs the light field distribution of the exit pupil plane of the objective lens of the confocal microscope system that is more in line with the actual situation.

Provided are a dark-field confocal microscopic measurement apparatus and method based on frequency mismatch demodulation, relating to a dark-field confocal microscopic measurement apparatus and method. The problems that lateral and axial resolutions are affected and the stability of a microscopic system is reduced because the traditional nondestructive testing technology of subsurface defects in dark-field confocal microscopic measurement relies on a complex beam shaping mechanism are solved. The apparatus includes a dual-channel waveform generator, a modulated illumination module, an optical scanning module, a mismatch demodulation module, and an axial displacement table. A sample is placed on the axial displacement table, and one channel of the dual-channel waveform generator is connected to the modulated illumination module. Laser emitted by the modulated illumination module irradiates the sample through the optical scanning module, and returned light of the sample is collected by the mismatch demodulation module.

The present application provides a method for preparing a Micro-LED device. The method improves the problem of sidewall damage caused by the use of dry etching to prepare Micro-LED grain structures. It is proposed to use a combination of tetramethylammonium hydroxide chemical corrosion and sidewall passivation to repair the sidewalls, which can completely etch the amorphous area of the Micro-LED sidewalls along the longitudinal direction, and has less destructive effect on the Micro-LED structure along the transverse direction. The sidewall lattice of the sample after etching is complete and the sidewalls are vertical. After the Micro-LED grains are formed, an optical evaluation method combining laser confocal microscopy and photoluminescence spectroscopy is used to evaluate their early performance. After packaging, the electrical performance of the Micro-LED device is tested, and the electrical performance test results are compared with the early luminescence performance evaluation. The present application can effectively process the Micro-LED sidewall damage layer, verticalize the Micro-LED sidewalls, and solve the problem of inconsistent areas of the upper and lower regions of the quantum well caused by the tilt of the sidewalls after dry etching.

The present application relates to the field of image processing, in particular to a kind of dynamic evaluation method and system of endothelial barrier integrity in organ chip.The method obtains the dual-channel fluorescence image of cell nucleus and intercellular junction protein by confocal microscope, and extracts four key feature parameters based on image processing technology: cell coverage, cell nucleus distribution uniformity, intercellular junction protein intensity and junction protein integrity.Through the fusion of these parameters, a comprehensive evaluation index is obtained, and compared with the preset threshold value, to determine whether the endothelial barrier is complete.The present application avoids the interference of traditional permeability detection method, realizes the multidimensional, quantitative and high reliability evaluation of barrier structure, and is suitable for various organ chip models.