179 results about "Confocal microscopy" patented technology

The invention discloses a quality detection method and system for vehicle safety belt production and a storage medium, and relates to the field of manufacturing quality control of automobile passive safety parts. The method aims at solving the problems that an existing detection means is weak in tiny defect recognition capacity, insufficient in defect evolution modeling and lack of intelligent feedback regulation and control. According to the method, multiple detection means such as medium-wave infrared thermal imaging, laser confocal microscopy, structured light three-dimensional reconstruction and capacitance sensing are fused, and multi-mode defect sensing of key parts such as a braid, an insertion area and an adhesive layer is achieved; deducing a defect evolution process and generating a high-credibility pseudo label by constructing a thermal-stress-rheology coupling finite element model; the graph neural network and the AI model are combined to complete defect type identification, risk quantification and grading, and automatic optimization of process parameters and production line control response are realized based on the quality grade, so that the detection precision, the response efficiency and the product yield are remarkably improved.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention relates to the technical field of medical image processing, and discloses an adversarial generative network model for three-dimensional reconstruction of a brain cell-level vascular network. The model comprises a three-dimensional blood vessel feature extraction module, a dynamic attention generator, a multi-scale discriminator and the like. Characteristics are obtained through multi-modal medical image data (magnetic resonance angiography and confocal microscope scanning data), and high-precision vascular network three-dimensional reconstruction is realized by utilizing cooperative work of all the modules. The dynamic attention generator calculates the topological connection probability of the vascular branches, and the multi-scale discriminator globally and locally evaluates the reconstruction result. Meanwhile, model training is optimized through an adversarial training controller, and the blood vessel network is perfected through a capillary network completion module. According to the method, the advantages of multi-modal data are effectively fused, the reconstruction precision is improved, and powerful support is provided for research and diagnosis of brain vascular diseases.

The invention relates to the technical field of quantum sensing and optical instruments, and discloses an integrated scanning confocal microscope for diamond NV color center quantum sensing, which comprises a control module, an integrated circuit module, an integrated laser module and an integrated light path module, the control module is used for exciting a diamond NV color center, the integrated light path module realizes two-dimensional scanning of a laser beam on a sample focal plane through a galvanometer, an objective lens is used for collecting NV color center fluorescence, meanwhile, a single-photon counter and a camera are arranged, confocal detection and wide-field microscopic imaging are realized, and the integrated circuit module receives an instruction of the control module. The microscope can realize confocal scanning fluorescence imaging, optical detection magnetic resonance spectrum measurement and NV color center spin coherent control experiments, such as Rabian oscillation, Rahm, Haln echo and spin relaxation experiments, and provides comprehensive experimental ability for diamond NV color center quantum sensing.

The invention provides a single-photon anti-bunching measurement method and system based on an ADC acquisition card. The single-photon anti-bunching measurement method and system are mainly used for acquiring and analyzing a second-order correlation function of a single-photon optical signal. Compared with a traditional single photon detection method, the high-speed ADC acquisition card in an intermediate trigger mode is adopted, redundant data are reduced, and meanwhile the data acquisition efficiency is improved. The system comprises a data acquisition unit, a data reading module, a data processing center and an algorithm execution module, and the data acquisition unit acquires a single photon signal through a confocal microscope system and converts an optical signal into an electric signal by using an SPAD detector. The ADC acquisition card adopts an intermediate trigger mode, so that sampling can be accurately triggered in a set effective sampling interval, and a large amount of redundant data existing in a traditional sampling mode is avoided; and the completeness of the time sequence is better ensured while the data is accurately acquired.

The invention relates to the technical field of crystal detection, in particular to a comprehensive crystal surface crack analysis method and system based on multiple characterization technologies, and the method comprises the following steps: obtaining a three-dimensional morphology graph of a crystal surface crack by using a laser confocal microscopy technology, and measuring crystal surface stress by using a Raman spectrum technology; a curvature tracking and edge detection algorithm is adopted to extract a crack main path, a Bezier curve is combined to fit the geometric morphology of the crack, the curvature, the trend and the branch characteristics of the crack are calculated, and geometric modeling of the crack path is completed; predicting a crack initiation area by using a graph structure neural network, and dividing a crystal surface risk area; predicting a crack expansion trend by combining a phase field evolution model and a machine learning method; and fusing the risk area and the expansion trend to generate a crack risk joint heat map. According to the method, the crack initiation area and the expansion trend can be accurately identified, and a scientific basis is provided for crystal cutting process optimization and crack control.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

This invention belongs to the field of fluorescence microscopy imaging technology, specifically a calculation method for high-density fluorescence fluctuation super-resolution microscopy imaging. This method dynamically controls the activation-quenching cycle of reversible fluorescent molecules through alternating sequential irradiation with activation and excitation light, effectively reducing the density of fluorescent molecules by combining activation light control and image differential processing techniques. Specifically, it includes: optimizing light control parameters such as frame rate, activation light power and exposure time, and excitation light power and exposure time; using an inter-frame difference algorithm to reduce the effective fluorescence density; and employing the SOFI algorithm for high-order cumulant calculation to achieve super-resolution reconstruction. Compared to existing technologies, this invention eliminates the need for complex multicolor labeling, significantly improves imaging resolution through a light control-differential synergistic strategy, and is compatible with various microscopy systems such as wide-field and confocal microscopy, providing an efficient and reliable technical solution for live-cell super-resolution observation.

This invention discloses a detection method, system, and medium based on line spectral confocalization. The method includes: determining the number of peaks; if the number of peaks is one, adjusting the light intensity value corresponding to the peak wavelength to be within the light intensity working range; if the number of peaks is not less than two, extracting the light intensity value corresponding to the peak wavelength, analyzing the peak morphology, and identifying the peak wavelength associated with each reflection region; analyzing the offset of the height of the specular reflection region, correcting the detection height of the specular reflection region to obtain the height difference between the reflection regions covered within the width of the line spot at any position on the scanning line; and adjusting the distance of the sensor relative to the surface of the object under test according to the height difference, so that there is one and only one valid peak in the spectral image at any position on the scanning line. This invention can accurately calculate the height difference between the specular reflection region and the diffuse reflection region covered within the width of the line spot, improving the signal-to-noise ratio of the spectral image and expanding the application scenarios.

The invention belongs to the technical field of in-situ observation sample preparation, and particularly relates to a method for in-situ observation of a Ti alloy sample through a high-temperature laser confocal microscopy, and the method comprises the following steps: S1, grinding, polishing and ultrasonic cleaning treatment of Ti alloy; s2, performing plasma spraying treatment on the alumina crucible, and spraying a layer of yttrium oxide powder on the surface of the alumina crucible; s3, hanging a pure titanium sheet in a hearth of the high-temperature laser confocal microscope; and S4, the Ti alloy sample pretreated in the step S1 is put into a crucible, and in-situ observation is conducted through a high-temperature laser confocal microscope. The method is simple to operate, solves the problems that the Ti alloy is easy to oxidize at high temperature and is not provided with an adaptive observation crucible, and can be used for observing the microstructure evolution of the Ti alloy in situ and observing the microstructure evolution process of the Ti alloy in real time under the high-temperature laser confocal microscope so as to facilitate the subsequent research on growth mechanism analysis and growth kinetics quantification.

The present application provides a method for preparing a Micro-LED device. The method improves the problem of sidewall damage caused by the use of dry etching to prepare Micro-LED grain structures. It is proposed to use a combination of tetramethylammonium hydroxide chemical corrosion and sidewall passivation to repair the sidewalls, which can completely etch the amorphous area of the Micro-LED sidewalls along the longitudinal direction, and has less destructive effect on the Micro-LED structure along the transverse direction. The sidewall lattice of the sample after etching is complete and the sidewalls are vertical. After the Micro-LED grains are formed, an optical evaluation method combining laser confocal microscopy and photoluminescence spectroscopy is used to evaluate their early performance. After packaging, the electrical performance of the Micro-LED device is tested, and the electrical performance test results are compared with the early luminescence performance evaluation. The present application can effectively process the Micro-LED sidewall damage layer, verticalize the Micro-LED sidewalls, and solve the problem of inconsistent areas of the upper and lower regions of the quantum well caused by the tilt of the sidewalls after dry etching.

The present application relates to the field of image processing, in particular to a kind of dynamic evaluation method and system of endothelial barrier integrity in organ chip.The method obtains the dual-channel fluorescence image of cell nucleus and intercellular junction protein by confocal microscope, and extracts four key feature parameters based on image processing technology: cell coverage, cell nucleus distribution uniformity, intercellular junction protein intensity and junction protein integrity.Through the fusion of these parameters, a comprehensive evaluation index is obtained, and compared with the preset threshold value, to determine whether the endothelial barrier is complete.The present application avoids the interference of traditional permeability detection method, realizes the multidimensional, quantitative and high reliability evaluation of barrier structure, and is suitable for various organ chip models.

The application discloses a lithium acetate-polyethylene glycol mediated protoplast lipid nanoparticle delivery method and belongs to the technical field of biology. 2+ )concentration to improve the PEG formula, successfully delivers the lipid nanoparticle (LNP) to the protoplast of oil palm, coconut and other palm crops, the mRNA of the nanoparticle is wrapped with enhanced green fluorescent protein (eGFP), and the fluorescent protein can be directly expressed in the protoplast with the mRNA as a template without a promoter. After the LNP is delivered to the protoplast of the palm crops by the method, green fluorescent signals are detected by laser confocal microscopy. The method can be used for directly delivering target gene mRNA to the protoplast of the palm crops, and can also deliver CRISPR / Cas system protein and the like for gene editing technology research.

1. The name of the design product: air floating platform. 2. The use of the design product: vibration isolation for high-precision measurement and experimental equipment, confocal microscope, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 2.

The present invention relates to the field of molecular biology technology, and in particular to a method for analyzing the interaction of 8:2 fluoromodulin alcohol-target protein based on confocal fluorescence imaging. The present invention utilizes Fmoc-Glu (OtBu) OH as a linker reagent and a linear alkane pollutant 8:2FTOH to produce an 8:2FTOH-Glu (OtBu)-FITC complex with strong green fluorescence, and then observes and analyzes the strength of the interaction between 8:2FTOH and a target protein (AHR) through confocal microscopy. The present invention is simple and intuitive, highly economical and easy to operate; compared with previous molecule-protein interaction technologies, the experimental conditions are simple and no additional instruments and equipment are required. At the same time, the interaction analysis method provided by the present invention can be used for the analysis of 8:2FTOH mediating multi-organ inflammation and damage by activating AHR.

The present invention discloses a high-precision spectral confocal microscopy imaging system, comprising a light modulation module, a spectrum splitting module, a scanning imaging module, an image detection device and a computer. The light beam emitted by the light modulation module passes through the scanning imaging module and is incident on a sample to excite a fluorescence signal. The fluorescence signal passes through the spectrum splitting module and is received by the image detection device. The image detection device converts the current signal into a digital signal and transmits it to the computer to complete imaging. The system is characterized in that the spectrum splitting module consists of a first converging lens, a first pinhole, a first linear long-wave pass filter, a second converging lens, a first linear short-wave pass filter and a third converging lens arranged in sequence. The system has the advantage of realizing high-precision spectral confocal imaging by combining lenses and linear filters on the basis of the traditional confocal microscopy imaging principle, solving the problem of spectral crosstalk in confocal imaging, and achieving a spectral resolution of less than 3.0 nm.

The utility model relates to a culture dish, in particular to a culture dish for a laser confocal microscope, which comprises a dish body and a cover body, the dish body is a cylinder with a shooting groove in the top surface; the cover body comprises an outer cylinder and an inner cylinder; the inner column body is arranged in the outer column body, and the top surface of the inner column body is not lower than the top surface of the outer column body; the outer column body is provided with an annular groove surrounding the inner column body, and the inner column body is provided with a culture groove; the cover body is detachably connected with the dish body; and when the cover body covers the opening in the top surface of the dish body, the culture groove and the shooting groove are oppositely arranged. Compared with the prior art, the culture dish for the laser confocal microscope solves the problems that a conventional culture dish in the prior art does not meet the requirements of the laser confocal microscope, and an existing culture dish for the laser confocal microscope is complex to use and tedious to operate. According to the scheme, the culture dish for the laser confocal microscope, which can be repeatedly used, has relatively small limitation on samples and can be directly used for experiments such as invasion and the like, is realized.

A radiotherapy system for treatment of skin includes a confocal microscopy imaging component and a photoacoustic microscopy imaging component to capture images of the skin of a patient at more than one depth. A processor combines images to produce a fused model for the region of interest, generates a plan for radiotherapy treatment based on the fused model, and controls the radiotherapy component for carrying out the radiotherapy treatment.

The application relates to the technical field of microscopes, in particular to a reflection type confocal microscope based on a perovskite detector. The reflection type confocal microscope comprises a laser, a polarizer, a polarization beam splitter prism, a galvanometer, a lens unit, a mirror, a sample display unit, a focusing lens and a perovskite detector; the laser, the polarizer, the polarization beam splitter prism, the galvanometer, the lens unit, the mirror and the sample display unit are sequentially connected to form an incident light channel; the sample display unit, the mirror, the lens unit, the galvanometer, the polarization beam splitter prism, the focusing lens and the perovskite detector are sequentially connected to form a reflected light channel. Compared with a traditional confocal microscope, the confocal microscope has the effects of high detection sensitivity, low cost and fast response speed.

The application discloses a monitoring method and system for three-dimensional phenotype parameters of stoma cells of living plants, and comprises the following steps: S1, a plant confocal three-dimensional microscopic imaging and photosynthetic parameter measuring device is used, a self-made photosynthetic leaf chamber is used to realize cooperative monitoring of a down-positioned confocal microscope and a portable photosynthetic instrument, morphological changes of the same stoma under different environments are monitored, and three-dimensional Z-stack images and physiological parameters of a stoma complex of the living plants are synchronously obtained; and S2, a fluorescence image is automatically segmented and three-dimensional surface reconstruction is performed by using a granularity-based active contour model, so that three-dimensional phenotype parameters and pore characteristics of the stoma complex are obtained.

This utility model relates to a sample holder for a high-temperature laser confocal microscope, comprising a drive shaft connecting part, a transition extension part, and a sample holding part connected in sequence. The drive shaft connecting part is connected to the drive shaft of the high-temperature laser confocal microscope. One end of the drive shaft is a screw structure, and the other end is fixedly connected to the transition extension part. The end of the sample holding part is provided with a pin hole, and the sample holding part is connected to the sample through the pin hole and a pin. The end of the sample holding part has a clamping slot. The advantages are: simple structure, convenient operation, suitable for VL2000DX high-temperature laser confocal microscope, enabling observation, measurement, and analysis of material microstructure / phase transformation processes, crack occurrence, direction, and high-temperature mechanical properties under stress in high-temperature environments. This utility model ensures that the sample is under uniaxial tensile stress, greatly reducing the difficulty of sample installation, and does not rotate during load application, facilitating in-situ observation.

The utility model provides a multi-point adsorption type scanning head and a confocal microscope, and belongs to the technical field of skin confocal microscope detection. According to the technical scheme, the device comprises a movable cover, a nasal tube and an objective lens, the movable cover is fixedly connected with the nasal tube, the objective lens is located in the nasal tube, a fixing ring is arranged on the outer side of the nasal tube, a plurality of extension rods are evenly arranged on the fixing ring at equal intervals, and elastic mechanisms are arranged at the ends, away from the fixing ring, of the extension rods; a rubber sucker is fixedly arranged at the open end of the mounting cover and communicated with the interior of the mounting cover, a three-way piece is arranged on the elastic mechanism, and an inlet of the three-way piece is communicated with the interior of the mounting cover through a hose. The beneficial effects of the utility model are that: the utility model discloses have little influence on the skin surface to be detected, not only can realize fast location, and can closely fit the skin, adopt the purely mechanical mode, reduce the electric equipment, and the adaptability to the skin is stronger.

The utility model relates to material high temperature analysis test field especially, it is a kind of temperature measuring device for high temperature confocal microscope, including insulating core (1), sample support assembly (2) is equipped on the insulating core (1), temperature sensor is equipped below the sample support assembly (2), the even silk (5) of temperature sensor is arranged in the inside of the insulating core (1).The utility model through novel structure design makes temperature sensor can directly detect the real temperature of sample crucible, avoids the measurement error while greatly improves the detection efficiency and stability.Meanwhile, the application has low use cost, is easy to process, has greater market promotion value.

The invention provides a non-treatment-purpose confocal detection method for chemical efficacy based on a zebra fish model and an application of the non-treatment-purpose confocal detection method. According to the application, the KRT4 transgenic red fluorescent line zebrafish is utilized, and UVA damage and image acquisition are carried out through a confocal microscope. The sunscreen effect is detected, the bottom of a culture dish is coated with a tested chemical to form a film, and the sunscreen effect is quantitatively evaluated by comparing the skin fluorescence intensity weakening degree of a sample group and a model group after UVA irradiation; and detecting the repair effect, incubating the damaged zebrafish by using a tested chemical solution, and quantitatively evaluating the repair effect by comparing the epidermis fluorescence intensity recovery degrees of the incubated sample group and the model group. According to the method, the living body biological model is combined with the confocal microscope UVA damage and imaging technology, direct, quantitative, visual and high-throughput evaluation on the efficacy of the chemicals is achieved, and the defects that a traditional in-vitro method is not close to living bodies, and human body tests are high in cost and long in period are overcome.

The invention discloses a method for high-resolution visual observation of dynamic interaction of proteins in depression-related brain region neurons. According to the method, virus delivery and PLA proximity ligation detection are combined; and a confocal microscope is used for shooting a fluorescent layer scanning picture, Image J is used for carrying out PLA signal point density statistics, and the dynamic interaction process of the proteins in the cerebral region neurons related to depression is observed. According to the invention, AAV virus delivery and PLA proximity ligation detection are combined for the first time, so that the problem of insufficient expression of target protein in brain tissues can be solved; the method is expected to break through the limitation of the prior art, a brand new tool is provided for accurate diagnosis and treatment of depression, and a new method is provided for visual observation of protein interaction of multiple brain regions and multiple types of cells in the brain region.

The present application discloses a dark-field confocal microscopy measurement device and method based on time-varying fractional-order vortex demodulation, relating to the field of confocal microscopy measurement technology. The device comprises a time-varying modulated illumination module, an optical scanning module, a signal acquisition and demodulation module, a function generator and a sample stage. The function generator is respectively connected to the time-varying modulated illumination module and the signal acquisition and demodulation module to provide reference signals to the time-varying modulated illumination module and the signal acquisition and demodulation module respectively. The sample stage is used to place a sample to be measured. The time-varying modulated illumination module is used to emit fractional-order vortex light to the optical scanning module. The optical scanning module is used to transmit the fractional-order vortex light to the sample to be measured in the sample stage and transmit the return light signal to the signal acquisition and demodulation module. The signal acquisition and demodulation module is used to collect the return light signal and perform dark-field confocal detection on the return light signal according to the reference signal to obtain measurement information of the sample to be measured. The present application can improve the sensitivity and accuracy of the measuring device.