310 results about "Confocal microscopy" patented technology

The invention discloses a quality detection method and system for vehicle safety belt production and a storage medium, and relates to the field of manufacturing quality control of automobile passive safety parts. The method aims at solving the problems that an existing detection means is weak in tiny defect recognition capacity, insufficient in defect evolution modeling and lack of intelligent feedback regulation and control. According to the method, multiple detection means such as medium-wave infrared thermal imaging, laser confocal microscopy, structured light three-dimensional reconstruction and capacitance sensing are fused, and multi-mode defect sensing of key parts such as a braid, an insertion area and an adhesive layer is achieved; deducing a defect evolution process and generating a high-credibility pseudo label by constructing a thermal-stress-rheology coupling finite element model; the graph neural network and the AI model are combined to complete defect type identification, risk quantification and grading, and automatic optimization of process parameters and production line control response are realized based on the quality grade, so that the detection precision, the response efficiency and the product yield are remarkably improved.

The invention discloses a confocal microscope camera calibration method based on high-precision motion control. The confocal microscope camera calibration method mainly comprises the steps of establishing four coordinate systems, placing a checkerboard calibration plate on a high-precision platform and adjusting the checkerboard calibration plate to the center of the view of a camera, and shooting and recording image coordinates of a calibration point. And based on the imaging relationship between the optical center of the camera and the object point, determining the mapping relationship between the coordinate systems so as to establish a preliminary imaging model. Then, nonlinear correction is performed on the imaging model, lens distortion is eliminated, and corrected image coordinates are converted into pixel coordinates. The non-linear correction parameters are optimized through the multi-view data, the mapping relation between the image plane and the platform physical plane is further calculated, and the mapping relation is optimized through the least square method so as to improve the calibration precision. Finally, pixel coordinates in the image are converted into physical coordinates of a platform through the optimized matrix, and support is provided for the automatic focusing process of the confocal microscope.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention discloses a confocal microscopic image distortion correction method based on a two-axis MEMS (Micro Electro Mechanical System) scanning galvanometer. The method comprises the following steps: acquiring an original image by using a confocal microscope of a two-axis MEMS scanning galvanometer; calculating to obtain a galvanometer normal vector; calculating to obtain an incident light unit vector; calculating to obtain a reflected light unit vector; according to the method, distortion introduced by the MEMS galvanometer can be removed, distortion introduced by the MEMS galvanometer can be corrected under the condition that the posture of the scanned end face is unknown, and then a distortionless confocal image is obtained.

The invention discloses a tomato LACS2 gene promoter and application thereof, and belongs to the technical field of biology. On one hand, the invention provides the tomato LACS2 gene promoter, and on the other hand, the invention provides application of the tomato LACS2 gene promoter in promoting target gene expression in plants. According to the invention, a tomato LACS2 gene promoter fragment (788bp) is constructed to drive an EGFP-GUS (Enhanced Green Fluorescent Protein-Glucuronidase) gene fusion expression vector. GUS histochemical staining and laser confocal microscopy observation prove that the promoter fragment can guide normal expression of the reporter gene in the tomato epidermis. Therefore, the LACS2 promoter can be used for researching development, quality improvement and postharvest preservation of tomato peels and specific expression of exogenous genes in the tomato peels.

According to embodiments of the present invention, a parallel scanning confocal microscope is provided. The microscope includes an optical circulator including a first port, a second port and a third port; a light source optically coupled to the first port; an optical arrangement in optical communication with the second port; a focusing lens; and a photodetector optically coupled to the third port. The light source may be configured to emit light towards the optical arrangement along an illumination path. The light may be directed as multiple optical beams towards the focusing lens, which may be configured to focus the beams to confocally and simultaneously illuminate a sample along the illumination path; and to collect light reflected from the sample confocally along a reflection path. The reflected light may be directed towards the photodetector along the reflection path. According to further embodiments, a parallel scanning confocal microscopy method is also provided.

The invention relates to a microscope system, in particular to a confocal microscope system of a short-wave infrared band. The system comprises a bright / dark field microscopy subsystem, a photon emission microscopy subsystem, a short wave infrared laser scanning confocal microscopy subsystem, a fluorescence confocal microscopy subsystem and a control module. According to the system provided by the invention, the short-wave infrared fluorescence lifetime curve can be obtained through the electro-luminescence emission and / or photoluminescence emission of the semiconductor device in the integrated circuit, so that the defect type of the semiconductor can be judged. Besides, the system adopts a multi-subsystem optical path multiplexing structure in structural design, short-wave infrared fluorescence generated by exciting a defect or a failure point is collected while observation is facilitated, high integration level is achieved, and observation, positioning and failure analysis of the defect or the failure point can be achieved only by using a single system provided by the invention.

The invention belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV (at) HP based on hyaluronic acid as well as a preparation method and application of the temperature-sensitive hydrogel SV (at) HP. The temperature-sensitive hydrogel SV (at) HP entraps CS-siRNA nanoparticles and VEGF (at) PLGA microspheres, and is used for realizing microenvironment responsive drug delivery to treat intrauterine adhesion. Chlorogenic acid and TGF beta1 siRNA are self-assembled through disulfide bonds to form CS-siRNA nano-particles, the CS-siRNA nano-particles are used for treating intrauterine adhesion through gene and chemical therapy, and cell and animal experiments prove that the CS-siRNA nano-particles have a good effect of improving intrauterine adhesion. Through evaluation of a real-time fluorescence quantification method, a western blot method, a cell scratch experiment, laser confocal microscope observation, inverted fluorescence microscope observation, an in-vivo experiment and the like, the SV-coated HP gel has the effects of resisting inflammation, improving endometrial fibrosis, promoting IUA rat endometrial angiogenesis and repair, inhibiting intrauterine adhesion and improving the intrauterine environment.

The present disclosure describes a turntable parallel scanning confocal microscope illumination light path, the illumination light path comprising a sleeve assembly having a light source and a field diaphragm, a lens barrel assembly having a collector lens, a beam splitting chamber having a turntable, a tube lens and a microscope objective, the light source being configured to form a first parallel beam entering the lens barrel assembly via the field diaphragm; the light source is imaged on the focal plane of the light collecting mirror; the turntable is configured to receive a second parallel light beam emitted from the tube lens, the second parallel light beam is configured to form a preset image on the turntable, the turntable is configured to receive a first parallel light beam emitted into the light splitting chamber through the lens barrel assembly, and the first parallel light beam is configured to form a light spot covering a small hole surface of the turntable on the turntable; the microscope objective is configured to be installed at the position of an image of the light source which is formed by the light beam emitted from the tube mirror and meets the preset requirement. According to the invention, the illumination light path of the turntable parallel scanning confocal microscope, which is simple and convenient to operate and can be conveniently and accurately assembled and adjusted, can be provided.

Embodiments are for a confocal microscope unit attached to a connection port of a microscope including: light sources which output irradiation light to a sample to be observed; photodetectors which detect observation light generated from a sample in response to the irradiation light; a scan mirror which scans the irradiation light on the sample and guides the observation light generated from the sample to the photodetectors; a scan lens which guides the irradiation light scanned by the scan mirror to the microscope optical system and guides the observation light focused by the microscope optical system to the scan mirror; a lens barrel to which the scan lens is fixed; an attachment portion which attaches the lens barrel to the connection port ; and a movable portion which supports the lens barrel so that an angle of the lens barrel with respect to the attachment portion is changeable.

The invention relates to the technical field of medical image processing, and discloses an adversarial generative network model for three-dimensional reconstruction of a brain cell-level vascular network. The model comprises a three-dimensional blood vessel feature extraction module, a dynamic attention generator, a multi-scale discriminator and the like. Characteristics are obtained through multi-modal medical image data (magnetic resonance angiography and confocal microscope scanning data), and high-precision vascular network three-dimensional reconstruction is realized by utilizing cooperative work of all the modules. The dynamic attention generator calculates the topological connection probability of the vascular branches, and the multi-scale discriminator globally and locally evaluates the reconstruction result. Meanwhile, model training is optimized through an adversarial training controller, and the blood vessel network is perfected through a capillary network completion module. According to the method, the advantages of multi-modal data are effectively fused, the reconstruction precision is improved, and powerful support is provided for research and diagnosis of brain vascular diseases.

The invention discloses a high-precision fluorescence modulation super-resolution microscopic imaging method which is applied to the technical field of optical microscopic imaging. According to the novel high-precision fluorescence modulation super-resolution imaging technology, first-order Bessel annular light with a smaller point spread function is used for replacing common annular light generated by modulating Gaussian light through a vortex phase plate in the traditional technology, and the problem that the resolution ratio is insufficient in an existing fluorescence subtraction microscopy technology can be solved. The problem of image distortion caused by a negative side lobe is solved, the visibility and authenticity of a fine structure in an image are enhanced, and when any type of laser scanning confocal microscopy system and a fluorescence subtraction super-resolution imaging technology are oriented, only a wavefront control light path comprising a spatial light modulator and a Fourier lens needs to be added in an optical system, and the optical system can be used for controlling the optical system. Compared with the traditional super-resolution technology, the technology has the advantages that the complexity and cost of the system are reduced, the harm of photobleaching is reduced, the technology can be combined with other imaging technologies for use, and the imaging precision and the imaging quality are improved.

The invention provides a method for machining a cutting edge through composite laser based on dynamic parameter adjustment and real-time detection, and relates to the technical field of cutter machining. COMSOL is used for conducting simulation of the composite laser reinforced cutting edge, a COMSOL simulation database is established to match preset parameters, and the preset parameters are matched with the COMSOL simulation database; the heat affected zone of the cutter material strengthened by the composite laser and the corrosion amount are directly displayed in a simulation result, so that the efficiency of strengthening the cutting edge of the cutter by the laser is greatly improved; an XZ two-dimensional high-precision micro-motion platform is used, the stability and the machining precision of a laser are guaranteed, meanwhile, a two-dimensional confocal microscope is used for observing and collecting data, the data are fed back to a COMSOL database in real time by adjusting an evaluation model P, machining parameters are continuously corrected, and the machining precision is improved. And meanwhile, the preparation precision of the cutting edge of the cutter can be accurately controlled by dynamically adjusting the threshold value Q, a laser machining parameter feedback mechanism and optimizing current laser machining parameter setting, so that an ideal cutting edge of the cutter is obtained.

The invention discloses a method for segmenting different strata series of stratified shale and identifying oiliness, and belongs to the field of oil-gas exploration. The invention discloses a strata division method based on XRF (X-Ray Fluorescence) mineral scanning and single polarization-fluorescence-laser confocal optical lens combined observation, which realizes accurate identification and segmentation of micron-sized strata boundaries of a layered rock core sample from different scales, and performs group component extraction on segmented independent strata. And carrying out chromatography-mass spectrometry analysis and FT-ICR MS analysis on the extracted saturated, aromatic, non-aromatic, leached and other family components. The TSF spectrum, light and heavy components, biomarker compounds, non-hydrocarbon compounds and other data obtained through the experiments are comprehensively analyzed, layering scale oiliness difference quantification and oil source comparison are achieved, and the shale oil micro-migration phenomenon is determined. The influence of the heterogeneity of the layered rock core sample on a result obtained by a traditional analysis method is overcome. According to the technology, direct evidences are provided for accurate prediction of shale oil desserts, exploration risks are reduced, and economic benefits are improved.

The invention relates to the technical field of fluorescence immunoassay, and discloses a quantum resonance fluorescence immunoassay medical detection method, which comprises the following steps: 1, carrying out fixation and permeation treatment on cells, respectively incubating the cells by using labeled different quantum dot-antibody compounds, specifically binding antibodies with corresponding target proteins in the cells, and detecting the target proteins in the cells; a labeled cell sample is obtained; 2, under a confocal microscope, laser with a specific wavelength is adopted to excite the marked cell sample, and fluorescence lifetime image data of different quantum dot emission wavelengths are collected; according to the method, the fluorescence lifetime image data of different quantum dot emission wavelengths are acquired through the confocal microscope, the position and content information of different target proteins can be clearly distinguished, simultaneous analysis and accurate positioning of multiple target proteins are realized, and an efficient means is provided for multi-protein research in a complex biological system.

This application provides a vortex dichroism dark-field confocal microscopy measurement apparatus based on spiral transformation. An opposite-order vortex beam generation module is configured to generate a mixed vortex beam, a sample scanning module is configured to irradiate a scanning position of a to-be-measured sample by using the mixed vortex beam, to obtain a sample reflection beam. A spiral transformation module is configured to spatially separate the sample reflection beam to obtain spatially separated beams. A multi-order detection module is configured to detect the spatially separated beams to obtain a vortex dichroism signal at the scanning position. When the scanning position of the to-be-measured sample is defect-free, the vortex dichroism signal is zero. Conversely, when defects are present at the scanning position, the vortex dichroism signal is non-zero. The positive and negative the vortex dichroism signal respectively correspond to the left-handed and right-handed chirality of the defects.

System for performing chemical spectroscopy on samples from the scale of nanometers to millimeters or more with a multifunctional platform combining analytical and imaging techniques including dual beam photo-thermal spectroscopy with confocal microscopy, Raman spectroscopy, fluorescence detection, various vacuum analytical techniques and / or mass spectrometry. In embodiments described herein, the light beams of a dual-beam system are used for heating and sensing.

The invention relates to the technical field of microscopes, in particular to a reflective confocal microscope based on a perovskite detector. The reflective confocal microscope comprises a laser, a polarizer, a polarization splitting prism, a galvanometer, a lens unit, a reflector, a sample display unit, a focusing lens and a perovskite detector. The laser, the polarizer, the polarization splitting prism, the galvanometer, the lens unit, the reflector and the sample display unit are sequentially connected to form an incident light channel; the sample display unit, the reflector, the lens unit, the galvanometer, the polarization splitting prism, the focusing lens and the perovskite detector are sequentially connected to form a reflected light channel. Compared with a traditional confocal microscope, the confocal microscope has the advantages of being high in detection sensitivity, low in cost and high in response speed.

This disclosure provides microscope-based systems and methods that significantly improve image contrast and have better vertical / horizontal resolution. This disclosure uses confocal microscopy in replacement of conventional fluorescent microscopy, for example, adding a spinning disk or a confocal microscopy scanning unit on the prior invention. Using a spinning disk vastly improves the speed of image acquisition (allowing for imaging of fast dynamic processes and live specimens), and considerably reduces photo damage. Disclosed herein are some embodiments to demonstrate how to set up the spinning disk microscopy system for image-guided illumination and photolabeling.

The invention provides a confocal microscope objective lens with high optical flux and long working distance, the confocal microscope objective lens comprises a first lens group and a second lens group which are sequentially arranged along the light transmission direction, the first lens group comprises a first lens, a second lens, a third lens and a fourth lens which are sequentially arranged along the light transmission direction, the first lens is a negative lens, and the second lens is a positive lens. The working distance is prolonged; the combination of the second lens, the third lens and the fourth lens has positive focal power and is used for focusing light, so that the objective lens obtains a larger numerical aperture; the second lens group comprises a sixth lens and a seventh lens which are sequentially arranged in the light transmission direction, and the combination of the sixth lens and the seventh lens has negative focal power and is used for enlarging the aperture of the light beam and reducing the angle of the incident light beam at the same time. According to the scheme, a view field of 12 mm is achieved, the numerical aperture reaches 0.5, the working distance reaches 17.7 mm, and excellent imaging performance is achieved while the large view field, the high resolution and the long working distance are considered.

The invention relates to a spectrum detection module and a confocal microscope. The spectrum detection module comprises a dispersion element and a first spectrum splitting device located on a light emitting surface of the dispersion element, the first spectrum splitting device specifically comprises a first imaging lens group, a first slit assembly, a first converging lens group and a first photoelectric detector which are sequentially arranged along a first optical axis from the light emitting surface of the dispersion element; the first slit assembly comprises a pair of first slit halves arranged at intervals in the direction perpendicular to the first optical axis and a first driving piece in driving connection with a first slit plate, and the first slit plate is used for moving in the arrangement direction under the action of the first driving piece. A first variable slit with variable width and / or position is formed on the exit pupil plane of the first imaging lens group, the entrance pupil plane of the first converging lens group coincides with the first variable slit, the first photoelectric detector is located on the exit pupil plane of the first converging lens group, and it can be understood that the arrangement direction is the same as the spectrum expansion direction.

The utility model relates to the technical field of microscopes, in particular to confocal microscope imaging equipment, which comprises a base and a confocal microscope body arranged on the upper surface of the base, a mounting plate is fixedly arranged on the side surface of the base, and a protection mechanism for protecting the confocal microscope body is arranged on the side surface of the mounting plate; the protection mechanism comprises a transparent protection cover slidably arranged on the side face of the mounting plate, a rectangular groove is formed in the side face of the mounting plate, and threaded columns are rotationally arranged on the inner top wall and the inner bottom wall of the rectangular groove. According to the confocal microscope imaging equipment, through the arrangement of the protection mechanism, after the confocal microscope body of the confocal microscope imaging equipment is used, the transparent protection cover can be driven to move downwards through the rotation of the driving motor, so that the confocal microscope body is effectively protected; therefore, the defect that the used confocal scanning microscope cannot be effectively protected in the prior art is effectively overcome.

The invention relates to a large depth-of-field photoacoustic fusion confocal microscopy system and method based on light field regulation, the system comprises an illumination system, a detection system and an imaging system, the illumination system comprises a phase modulation optical assembly and an illumination beam power measurement assembly, the phase modulation optical assembly comprises a first lens, a half-wave plate, a first polarization splitting prism PBS, a second lens, a first quarter-wave plate and an optical module, and the optical module is used for modulating a diffusion function of an illumination point to generate a self-acceleration light beam; the self-accelerating beam is generated based on a cubic phase or an exponential phase or a transformed exponential phase. Compared with the prior art, the method has the advantages that the problem that high depth resolution and high resolution cannot be simultaneously achieved in chip defect detection is solved, and the like.

The invention discloses a method for characterizing the degree of fusion of new and old asphalt based on fluorescence microscopy, and the process is as follows: using a confocal microscope to observe the surface of a wood simulated aggregate coated with homogenous SBS modified asphalt, obtaining fluorescence images at different depths, calculating an average fluorescence intensity curve, and fitting a fluorescence intensity attenuation correction function; observing the mixed asphalt on the surface of the wood simulated aggregate under the confocal microscope; scanning from the outermost layer to the innermost layer of the wood simulated aggregate, and outputting a plurality of fluorescence images of the new and old asphalt in the asphalt film thickness layer; performing noise reduction processing on the fluorescence image, obtaining a concentration change curve of the new asphalt and correcting it using an attenuation correction function; calculating a concentration attenuation change curve of the new asphalt, judging the influence of noise and the asphalt fusion layer, retaining the layer position with a fitting coefficient less than or equal to 95%, and calculating the concentration attenuation change curve after removing the influence of noise and fluorescence signal attenuation; and defining the degree of fusion of the mixed asphalt by an area method.

Low-cost, portable in-vivo confocal ophthalmoscopes (PICOs) and associated methods are described that are capable of producing microscopic images of the anterior segment of the eye and the external eye structures enabling visualization of cellular and some sub-cellular structures. Some of the features of the described ophthalmoscopes include the use of a non-contact objective lens in conjunction with a grating-based, scan-less confocal microscopy approach, integration of the capability of imaging the external eye and anterior segment en face, integration of a display as part of the microscope, increased signal level and imaging speed, reduction of detected specular reflection, and attachment methods onto a slit lamp biomicroscope or for hand-held use.

The invention relates to the technical field of quantum sensing and optical instruments, and discloses an integrated scanning confocal microscope for diamond NV color center quantum sensing, which comprises a control module, an integrated circuit module, an integrated laser module and an integrated light path module, the control module is used for exciting a diamond NV color center, the integrated light path module realizes two-dimensional scanning of a laser beam on a sample focal plane through a galvanometer, an objective lens is used for collecting NV color center fluorescence, meanwhile, a single-photon counter and a camera are arranged, confocal detection and wide-field microscopic imaging are realized, and the integrated circuit module receives an instruction of the control module. The microscope can realize confocal scanning fluorescence imaging, optical detection magnetic resonance spectrum measurement and NV color center spin coherent control experiments, such as Rabian oscillation, Rahm, Haln echo and spin relaxation experiments, and provides comprehensive experimental ability for diamond NV color center quantum sensing.

The invention provides a single-photon anti-bunching measurement method and system based on an ADC acquisition card. The single-photon anti-bunching measurement method and system are mainly used for acquiring and analyzing a second-order correlation function of a single-photon optical signal. Compared with a traditional single photon detection method, the high-speed ADC acquisition card in an intermediate trigger mode is adopted, redundant data are reduced, and meanwhile the data acquisition efficiency is improved. The system comprises a data acquisition unit, a data reading module, a data processing center and an algorithm execution module, and the data acquisition unit acquires a single photon signal through a confocal microscope system and converts an optical signal into an electric signal by using an SPAD detector. The ADC acquisition card adopts an intermediate trigger mode, so that sampling can be accurately triggered in a set effective sampling interval, and a large amount of redundant data existing in a traditional sampling mode is avoided; and the completeness of the time sequence is better ensured while the data is accurately acquired.

The invention discloses an online optical clear solution for three-dimensional structure imaging of a large-volume biological sample, which is characterized in that the solution comprises iohexol, urea, 1, 2-propylene glycol, dimethyl sulfoxide, 3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid inner salt and water. The invention further discloses a three-dimensional imaging method for the large-volume biological sample. The method comprises the following steps: carrying out transparentizing treatment on the biological sample by using the online optical transparentizing solution, and carrying out three-dimensional imaging on the transparentized biological sample by using a fluorescence microscope. The fluorescence microscope comprises a confocal microscope and / or a light sheet microscope. When the online optical transparent solution is used for imaging, the transparent depth of a surface sample is large, the single imaging depth can be increased, and the cutting thickness can be improved, so that the cutting frequency is reduced, and finally the data acquisition efficiency of a large-volume sample is improved.

The invention discloses a deep learning feature-based automatic identification method for a firing pin trace, which is used for realizing accurate and automatic identification of the firing pin trace. The method comprises the following steps of: acquiring firing pin trace sample data by using a three-dimensional confocal microscope; filtering the acquired firing pin trace sample by using a spline filter to obtain firing pin trace surface roughness texture features; in consideration of an initial phase between the samples, keeping the reference sample unchanged, and rotating the to-be-detected sample in a range of [-180 degrees, 180 degrees] by taking 3 degrees as a step length; the reference sample and all the to-be-tested samples obtained through rotation are subjected to matching through a Detector-Free Local Feature Matching with Transformers (LoFTR) algorithm, and a combination with the largest number of matched feature points is selected as a preliminary matching result; in order to further improve the matching precision, the Locality Preserving Matching (LPM) technology is introduced on the basis to carry out wrong matching filtering on the preliminary matching result, so that a more reliable matching result is obtained. According to the automatic pin firing trace recognition method based on the deep learning features, whether two bullet shells are from the same gun or not can be judged according to the number of the matched feature points, and then auxiliary evidence is provided for detection of gun-related cases.