117 results about "Confocal microscopy" patented technology

The invention relates to the technical field of quantum sensing and optical instruments, and discloses an integrated scanning confocal microscope for diamond NV color center quantum sensing, which comprises a control module, an integrated circuit module, an integrated laser module and an integrated light path module, the control module is used for exciting a diamond NV color center, the integrated light path module realizes two-dimensional scanning of a laser beam on a sample focal plane through a galvanometer, an objective lens is used for collecting NV color center fluorescence, meanwhile, a single-photon counter and a camera are arranged, confocal detection and wide-field microscopic imaging are realized, and the integrated circuit module receives an instruction of the control module. The microscope can realize confocal scanning fluorescence imaging, optical detection magnetic resonance spectrum measurement and NV color center spin coherent control experiments, such as Rabian oscillation, Rahm, Haln echo and spin relaxation experiments, and provides comprehensive experimental ability for diamond NV color center quantum sensing.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

This invention belongs to the field of fluorescence microscopy imaging technology, specifically a calculation method for high-density fluorescence fluctuation super-resolution microscopy imaging. This method dynamically controls the activation-quenching cycle of reversible fluorescent molecules through alternating sequential irradiation with activation and excitation light, effectively reducing the density of fluorescent molecules by combining activation light control and image differential processing techniques. Specifically, it includes: optimizing light control parameters such as frame rate, activation light power and exposure time, and excitation light power and exposure time; using an inter-frame difference algorithm to reduce the effective fluorescence density; and employing the SOFI algorithm for high-order cumulant calculation to achieve super-resolution reconstruction. Compared to existing technologies, this invention eliminates the need for complex multicolor labeling, significantly improves imaging resolution through a light control-differential synergistic strategy, and is compatible with various microscopy systems such as wide-field and confocal microscopy, providing an efficient and reliable technical solution for live-cell super-resolution observation.

This invention discloses a detection method, system, and medium based on line spectral confocalization. The method includes: determining the number of peaks; if the number of peaks is one, adjusting the light intensity value corresponding to the peak wavelength to be within the light intensity working range; if the number of peaks is not less than two, extracting the light intensity value corresponding to the peak wavelength, analyzing the peak morphology, and identifying the peak wavelength associated with each reflection region; analyzing the offset of the height of the specular reflection region, correcting the detection height of the specular reflection region to obtain the height difference between the reflection regions covered within the width of the line spot at any position on the scanning line; and adjusting the distance of the sensor relative to the surface of the object under test according to the height difference, so that there is one and only one valid peak in the spectral image at any position on the scanning line. This invention can accurately calculate the height difference between the specular reflection region and the diffuse reflection region covered within the width of the line spot, improving the signal-to-noise ratio of the spectral image and expanding the application scenarios.

The invention belongs to the technical field of in-situ observation sample preparation, and particularly relates to a method for in-situ observation of a Ti alloy sample through a high-temperature laser confocal microscopy, and the method comprises the following steps: S1, grinding, polishing and ultrasonic cleaning treatment of Ti alloy; s2, performing plasma spraying treatment on the alumina crucible, and spraying a layer of yttrium oxide powder on the surface of the alumina crucible; s3, hanging a pure titanium sheet in a hearth of the high-temperature laser confocal microscope; and S4, the Ti alloy sample pretreated in the step S1 is put into a crucible, and in-situ observation is conducted through a high-temperature laser confocal microscope. The method is simple to operate, solves the problems that the Ti alloy is easy to oxidize at high temperature and is not provided with an adaptive observation crucible, and can be used for observing the microstructure evolution of the Ti alloy in situ and observing the microstructure evolution process of the Ti alloy in real time under the high-temperature laser confocal microscope so as to facilitate the subsequent research on growth mechanism analysis and growth kinetics quantification.

The present application relates to the field of image processing, in particular to a kind of dynamic evaluation method and system of endothelial barrier integrity in organ chip.The method obtains the dual-channel fluorescence image of cell nucleus and intercellular junction protein by confocal microscope, and extracts four key feature parameters based on image processing technology: cell coverage, cell nucleus distribution uniformity, intercellular junction protein intensity and junction protein integrity.Through the fusion of these parameters, a comprehensive evaluation index is obtained, and compared with the preset threshold value, to determine whether the endothelial barrier is complete.The present application avoids the interference of traditional permeability detection method, realizes the multidimensional, quantitative and high reliability evaluation of barrier structure, and is suitable for various organ chip models.

1. The name of the design product: air floating platform. 2. The use of the design product: vibration isolation for high-precision measurement and experimental equipment, confocal microscope, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 2.

The utility model relates to a culture dish, in particular to a culture dish for a laser confocal microscope, which comprises a dish body and a cover body, the dish body is a cylinder with a shooting groove in the top surface; the cover body comprises an outer cylinder and an inner cylinder; the inner column body is arranged in the outer column body, and the top surface of the inner column body is not lower than the top surface of the outer column body; the outer column body is provided with an annular groove surrounding the inner column body, and the inner column body is provided with a culture groove; the cover body is detachably connected with the dish body; and when the cover body covers the opening in the top surface of the dish body, the culture groove and the shooting groove are oppositely arranged. Compared with the prior art, the culture dish for the laser confocal microscope solves the problems that a conventional culture dish in the prior art does not meet the requirements of the laser confocal microscope, and an existing culture dish for the laser confocal microscope is complex to use and tedious to operate. According to the scheme, the culture dish for the laser confocal microscope, which can be repeatedly used, has relatively small limitation on samples and can be directly used for experiments such as invasion and the like, is realized.

A radiotherapy system for treatment of skin includes a confocal microscopy imaging component and a photoacoustic microscopy imaging component to capture images of the skin of a patient at more than one depth. A processor combines images to produce a fused model for the region of interest, generates a plan for radiotherapy treatment based on the fused model, and controls the radiotherapy component for carrying out the radiotherapy treatment.

The application discloses a monitoring method and system for three-dimensional phenotype parameters of stoma cells of living plants, and comprises the following steps: S1, a plant confocal three-dimensional microscopic imaging and photosynthetic parameter measuring device is used, a self-made photosynthetic leaf chamber is used to realize cooperative monitoring of a down-positioned confocal microscope and a portable photosynthetic instrument, morphological changes of the same stoma under different environments are monitored, and three-dimensional Z-stack images and physiological parameters of a stoma complex of the living plants are synchronously obtained; and S2, a fluorescence image is automatically segmented and three-dimensional surface reconstruction is performed by using a granularity-based active contour model, so that three-dimensional phenotype parameters and pore characteristics of the stoma complex are obtained.

This utility model relates to a sample holder for a high-temperature laser confocal microscope, comprising a drive shaft connecting part, a transition extension part, and a sample holding part connected in sequence. The drive shaft connecting part is connected to the drive shaft of the high-temperature laser confocal microscope. One end of the drive shaft is a screw structure, and the other end is fixedly connected to the transition extension part. The end of the sample holding part is provided with a pin hole, and the sample holding part is connected to the sample through the pin hole and a pin. The end of the sample holding part has a clamping slot. The advantages are: simple structure, convenient operation, suitable for VL2000DX high-temperature laser confocal microscope, enabling observation, measurement, and analysis of material microstructure / phase transformation processes, crack occurrence, direction, and high-temperature mechanical properties under stress in high-temperature environments. This utility model ensures that the sample is under uniaxial tensile stress, greatly reducing the difficulty of sample installation, and does not rotate during load application, facilitating in-situ observation.

The utility model relates to material high temperature analysis test field especially, it is a kind of temperature measuring device for high temperature confocal microscope, including insulating core (1), sample support assembly (2) is equipped on the insulating core (1), temperature sensor is equipped below the sample support assembly (2), the even silk (5) of temperature sensor is arranged in the inside of the insulating core (1).The utility model through novel structure design makes temperature sensor can directly detect the real temperature of sample crucible, avoids the measurement error while greatly improves the detection efficiency and stability.Meanwhile, the application has low use cost, is easy to process, has greater market promotion value.

The invention provides a non-treatment-purpose confocal detection method for chemical efficacy based on a zebra fish model and an application of the non-treatment-purpose confocal detection method. According to the application, the KRT4 transgenic red fluorescent line zebrafish is utilized, and UVA damage and image acquisition are carried out through a confocal microscope. The sunscreen effect is detected, the bottom of a culture dish is coated with a tested chemical to form a film, and the sunscreen effect is quantitatively evaluated by comparing the skin fluorescence intensity weakening degree of a sample group and a model group after UVA irradiation; and detecting the repair effect, incubating the damaged zebrafish by using a tested chemical solution, and quantitatively evaluating the repair effect by comparing the epidermis fluorescence intensity recovery degrees of the incubated sample group and the model group. According to the method, the living body biological model is combined with the confocal microscope UVA damage and imaging technology, direct, quantitative, visual and high-throughput evaluation on the efficacy of the chemicals is achieved, and the defects that a traditional in-vitro method is not close to living bodies, and human body tests are high in cost and long in period are overcome.

A scanning confocal photon-reassignment microscope is configured to operate in a coherent (reflectance or transmission) imaging mode and having a confocal pinhole the diameter of which is between 2 and 4 Airy units. The microscope can be used to observe viral particles in suspension.

This invention provides an electrically controlled microsphere-enhanced liquid crystal layer, a differential confocal microscopy imaging method and system. The electrically controlled microsphere-enhanced liquid crystal layer includes an electrically controlled liquid crystal layer and a microsphere array disposed on the electrically controlled liquid crystal layer. The method includes the following steps: converting a single-wavelength parallel beam into circularly polarized light, focusing it through an objective lens and an objective lens driver, and scanning the sample through the electrically controlled microsphere-enhanced liquid crystal layer; applying a voltage to the electrically controlled microsphere-enhanced liquid crystal layer; collecting the reflected or scattered return beam from the sample, splitting it into two probe beam paths, and generating corresponding first and second images; calculating the image quality evaluation value of the two-dimensional lateral image based on this, and using this as a feedback signal to apply a voltage to the electrically controlled microsphere-enhanced liquid crystal layer. This invention introduces an electrically controlled microsphere-enhanced liquid crystal layer into the imaging path of a confocal microscope, achieving a significant enhancement in lateral resolution. By controlling the liquid crystal with an applied voltage, the equivalent refractive index can be dynamically adjusted.

1. Name of the product in this design: In-vivo reflective confocal microscope imaging head (RCT-9503). 2. Purpose of this design: In medical institutions, it is used to collect, store, retrieve, display, and convert images of unstained epithelial cells and tissues on the body surface to assist doctors in clinical diagnosis. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This application relates to the technical field of optical precision measurement and discloses a dark-field confocal microscopy measurement apparatus and method based on aperture scanning of vortex illumination. The apparatus includes a vortex illumination generation module, a vortex illumination aperture scanning module, a sample scanning module, and a dark-field lock-in detection module. On one hand, the vortex illumination aperture scanning module enables the light spot focused on the sample to scan a small range of the sample, generating oscillating signals that highlight the scattering signal of nano-scale defects on the sample. On the other hand, highly-sensitive oscillation signal detection is achieved using the dark-field lock-in detection module. In the present disclosure, the small-range aperture scanning of vortex illumination is combined with the dark-field lock-in detection to realize highly-sensitive detection of defects smaller than 50 nm.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The application discloses a kind of identification and evaluation method for monitoring demulsification on-line by laser confocal microscope, and relates to the identification and evaluation technical field of demulsification process, the method is loaded emulsion by special on-line observation sample cell, and time reference is initialized synchronously by accurately injecting demulsifier by micro-injection pump, Z axis scanning is carried out according to time sequence using laser confocal microscope, and three-dimensional image data stack is obtained by automatically adjusting acquisition frequency.Extracting key kinetic parameters such as droplet coalescence rate and phase separation speed by dynamic three-dimensional reconstruction and quantitative analysis, the objective quantitative evaluation of demulsifier efficiency is realized by combining parameter variation trend, and dynamic three-dimensional rendering video and parameter curve can also be generated.The method has short experimental period and repeatable results, and is suitable for crude oil dehydration, sewage treatment and other fields, to provide scientific data support for demulsifier screening and process optimization.

The invention discloses a soft tissue thick slice preparation and high-resolution multicolor fluorescent three-dimensional imaging method. The method comprises the following steps: step 1, carrying out fixation and freezing protection treatment on a sample; step 2, putting the sample treated in the step 1 into an embedding medium, incubating and embedding to form an embedded block, and freezing and slicing the embedded block; the embedding medium comprises gelatin with a mass concentration of 8%, polyvinylpyrrolidone with a mass concentration of 2%, sucrose with a mass concentration of 20% and the balance of a phosphate buffer solution; step 3, unfreezing the slices in the step 2 in a room temperature environment, then carrying out pretreatment, antibody incubation and immunofluorescence staining, and carrying out pretreatment, antibody incubation and cell nucleus labeling in a dark environment; 4, performing three-dimensional imaging on the sample sliced in the step 3 by adopting a turntable confocal microscope to obtain three-dimensional data of tissue fluorescence distribution; however, the polyvinylpyrrolidone inhibits the formation and growth of ice crystals in the freezing process of the embedded block, and ensures the accuracy of the dyeing effect.

The utility model relates to a coupling module and a confocal microscope-optical tweezers integrated system, and relates to the technical field of optical instruments, and the coupling module comprises a housing which is provided with an installation space which longitudinally penetrates through the housing; a light through hole is formed in the side wall of the mounting space; the dichroscope frame is horizontally and linearly connected in the mounting space in a sliding manner and is aligned with the light through hole; the optical filter frame is located in the installation space and installed on the dichroscope frame. A dichroscope frame and an optical filter frame in the coupling module can slide, so that the optical tweezers module optical path and the confocal microscope optical path can be quickly coupled and separated.

The invention provides an early-stage focus multi-mode imaging observation endoscope system, and belongs to the technical field of medical apparatuses and instruments. An integrated electronic endoscope; the integrated electronic endoscope internally comprises an image processing device, an ultrasonic processor, a display, a natural light source device and a composite optical fiber, the system can quickly switch a white light mode and a narrow-band light mode, and tiny blood vessel abnormalities on the mucous membrane surface layer can be clearly shown; when a suspicious area is found, ultrasonic and opto-acoustic synchronous imaging can be started instantly, and the deep infiltration condition and metabolic activity information of a focus are obtained noninvasively; when pathology-level confirmation is needed, a multi-mode cooperative working mechanism of confocal microscopic imaging, in-vivo optical biopsy and direct observation of cellular morphology and glandular canal structures can be started, so that focuses, such as early cancerization and precancerous lesions, which are easy to be missed in diagnosis by a traditional endoscope can be found and identified earlier and more accurately, and the missed diagnosis rate and the misdiagnosis rate are remarkably reduced.

This invention discloses the promoter of the rice Os04g0650800 gene and its applications, belonging to the field of plant biotechnology. By cloning the promoter sequence of the Os04g0650800 gene, a recombinant expression vector pPGDH-YFP driving the yellow fluorescent protein gene was successfully constructed. Positive rice plants were screened by infecting Nipponbare rice with Agrobacterium. The T0 generation of positive rice seedlings were inoculated with Xanthomonas oryzae pv. oryzae. Xoo Using a confocal microscope, it was discovered that... Xoo Infection of positive rice plants can induce YFP expression. Genetic engineering of rice using this promoter, such as regulating the efficient expression of target genes through this promoter, will yield positive results. Xoo Infected rice plants; if this promoter is used to drive the expression of disease resistance-related genes in rice, rice plants resistant to bacterial blight will be obtained.

The invention belongs to the technical field of material detection, and particularly relates to a high-temperature tensile in-situ analysis method for 316H austenitic stainless steel, which adopts a high-temperature laser confocal microscope to monitor the formation and expansion process of cracks in real time. The method specifically comprises the following steps: 1) mounting a 316H austenitic stainless steel sample on a clamp of a transmission shaft in a tension-compression furnace, and adjusting the angle of the clamp to ensure that a clamping seam is in a horizontal state; (2) starting a tension and compression controller of the tension and compression furnace, and eliminating loading stress generated when the sample is mounted; according to the method, on the premise that the original state of the material is not damaged, dynamic monitoring of crack propagation and observation, measurement and analysis of the high-temperature mechanical property of the material can be achieved, and more visual and accurate experimental data are provided for researchers.

The invention discloses a temperature gradient optimization method for a high-temperature confocal microscope sample, and belongs to the technical field of metallurgical materials. The invention provides an improved method capable of accurately measuring, quantitatively evaluating and effectively reducing the temperature gradient aiming at the temperature gradient problem of a high-temperature laser confocal microscope sample in the heating process. The method comprises the following specific steps: processing a sample into a cylinder, polishing, welding a calibration thermocouple on the upper surface of the sample, and placing in a crucible; high-purity argon is filled for protection in a three-extraction and three-discharge mode; heating according to a set heat treatment system, collecting temperature data of a thermocouple welded on the upper surface of the sample and a temperature control thermocouple in the furnace at the same time in a heat preservation stage, and calculating a temperature difference value and a temperature gradient; adjusting the height of the sample, repeating the test, establishing a corresponding relation between the height and the temperature gradient, and selecting the height of the sample with the minimum temperature gradient as an optimized size. Through a simple mode of adjusting the height of the sample, the temperature gradient of the upper surface and the lower surface of the sample is effectively reduced, it is ensured that the sample is in a uniform temperature state in the heating and cooling process, the oxidation problem caused by traditional slow heating or long-time heat preservation is avoided, and reliable test data are provided for in-situ research of the material microstructure.

The application discloses a liver organoid culture chip, a liver organoid model and a preparation method and application thereof. The preparation method of the chip comprises the following steps: obtaining a film with multiple perforations; coating a cell low-adhesion material on the bottom of a cell culture plate, then covering the film on the bottom of the cell culture plate to obtain a patterned substrate; adding a cell-specific adhesion material to the patterned substrate for coating to obtain the chip; inoculating foregut cells in the liver organoid culture chip, removing the film with multiple perforations after the cells adhere, aspirating the culture medium in the liver organoid culture chip, and adding a first culture medium for maintaining culture for 3-5 days after washing; then, a second culture medium and a third culture medium are used in sequence to maintain culture, and the liver organoid model is obtained. The liver organoid model has high uniformity and can be imaged in situ, and is suitable for optical imaging instruments such as a confocal microscope.

This invention discloses a device, method, and system for determining the effective measurement area of ​​differential confocal axial measurement, relating to the field of confocal microscopy. The method includes: acquiring a surface image of the sample under test and a preset axial measurement range; extracting a standard measurement grayscale interval of the differential image according to the preset axial measurement range, and determining a first measurable grayscale interval of the pre-focus image and a second measurable grayscale interval of the post-focus image; removing image regions outside the standard measurement grayscale intervals in the differential image; performing an intersection operation on the first and second measurable grayscale intervals; filtering out invalid measurement regions in the removed differential image based on the intersection grayscale intervals, and using the effective measurement area in the removed differential image as the effective measurement area of ​​the differential confocal axial measurement method. The method of this invention can predict the effective measurement area of ​​the differential confocal axial measurement method, improving the data accuracy of the differential confocal axial measurement method in actual measurement processes.