5results about How to "Achieve qualitative" patented technology

本发明涉及生物检测领域，具体涉及一种稻黄单胞菌抗体检测试剂盒及其应用，本申请提供了一种稻黄单胞菌Aoutermembrane protein XadA抗原的单克隆抗体2A5，其包含如SEQ ID NO：2所示的重链可变区和如SEQ ID NO：6所示的轻链可变区，采用包含单克隆抗体2A5的检测试剂盒可实现稻黄单胞菌的定性检测以及对稻黄单胞菌胞外膜蛋白XadA抗原的定量检测，具有较高的特异性和灵敏度。

The application belongs to the technical field of biological medicine, and provides a mouse anti-human PRCC monoclonal paired antibody, a hybridoma cell strain and application thereof. The application provides hybridoma cell strains 5F9C2 (CCTCC NO: C2025312) and 15F7B5 (CCTCC NO: C2025341) which respectively secrete antibodies with unique heavy chain / light chain variable regions; the antibodies can be used for immunohistochemical and immunoblotting detection of PRCC alone, and can also be paired to establish a sandwich method system through an enzyme-linked immunosorbent assay. The antibodies provided by the application are high in specificity and sensitivity, and can be used for accurate qualitative and quantitative analysis of PRCC.

This invention discloses a method and application for colorimetric analysis of antibiotics using a paper-based detection chip. The method includes: preparing a paper-based detection unit; immersing the paper-based detection unit in a NiCo@C HNs solution modified with nucleic acid aptamers; drying to obtain a paper-based detection chip; adding samples containing different concentrations of antibiotics for reaction; then adding a solution containing TMB and H2O2 for colorimetric reaction; using a mobile phone to identify color intensity to obtain RGB or grayscale values; selecting the linear curve with the highest fitting degree between antibiotic concentration and RGB or grayscale values ​​as the antibiotic detection standard curve; and then quantitatively detecting the antibiotic content based on the RGB or grayscale values ​​of the measured sample. Antibiotics can also be qualitatively detected based on colorimetric analysis. This method has advantages such as low cost, simple operation, fast response, strong anti-interference ability, and on-site detection capability, and can be applied to the detection of antibiotic residues in the environment and food.

The application discloses a kind of equipment simulation model comprehensive credibility test and evaluation method, comprising: S1, multiple index system initial configuration is preset;S2, general test case set and the characteristic test case set for equipment model are constructed;S3, multiple credibility evaluation calculation method is preset and equipment model credibility index is calculated based on the multiple credibility evaluation calculation method;S4, multiple computing programs are preset and equipment model credibility evaluation result is calculated and output based on the multiple computing programs.It is also disclosed that system, device, electronic equipment and computer readable storage medium.

The application discloses a diesel engine under-charge real-time monitoring and fault diagnosis method, which measures the parameters such as cylinder pressure and crank angle signals of the diesel engine by comprehensively applying various sensors, firstly reads or calculates the cylinder pressure at the top dead center, the maximum explosion pressure, the effective power feature and the average effective pressure, respectively, calculates the difference value of the above parameters and the threshold value of the corresponding normal working condition, respectively, and preliminarily diagnoses whether the diesel engine is under-charge; secondly, the fault degree of each parameter is judged by calculating the deviation rate of each parameter; finally, the overall fault degree is comprehensively analyzed according to the above fault degree; according to the fusion of the fault characteristic information, the typical fault characteristics are compared, so that the composite fault diagnosis of the diesel engine is realized, various characteristic information is fully utilized, the diagnosis speed and accuracy are improved, qualitative and quantitative analysis is realized, and the application of the diesel engine fault diagnosis level will be improved.