An immunization grouping analysis and detection method based on
quantum point belongs to the nano-material field, comprises the following steps: (1) dispersing
quantum points into
borate buffer solution, adding sulfurated
diimine and second
antibody, mixing, reacting, centrifugating, washing, and obtaining second
antibody compound with
quantum point marks; wherein, the
mole ratio of the quantum points, the sulfurated
diimine and the second
antibody is: 60: 15: 1; or (2) dispersing the quantum points into
phosphoric acid buffer solution containing 1.25 % of
glutaraldehyde by
volume fraction, dispersing, reacting, adding second antibody, reacting, centrifugating, washing, and obtaining second antibody compound with quantum point marks; wherein, the
mole ratio of the quantum points, the
glutaraldehyde and the second antibody is: 60: 15: 1; then performing immunization reaction and observation according to the standard immunization grouping detection method. The method in the invention performs observation and judgement directly by
fluorescence microscope, is simple and easy, can increase the
fluorescence stability, and improve analysis accuracy and detection sensitivity.