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Quantum dot fluorescence coded microsphere and preparation method thereof

A technology of fluorescent coding and quantum dots, which is applied in the field of quantum dot fluorescent coding microspheres and its preparation, can solve the problems of reducing the sensitivity of fluorescent coding microspheres and unstable fluorescence intensity of coding microspheres, achieve uniform emission wavelengths, and improve fluorescence stability resistance, reducing the effect of self-absorption

Active Publication Date: 2020-11-17
广州珈源新材料有限公司
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  • Description
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Problems solved by technology

[0004] The problem to be solved by the present invention is that the quantum dot fluorescent coded microspheres prepared based on swelling method and covalent binding method in the prior art have unstable fluorescence intensity of coded microspheres, and the reduction due to strong non-specific adsorption of quantum dot microspheres Defects such as reduced sensitivity of fluorescently encoded microspheres caused by non-specific adsorption treatment

Method used

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  • Quantum dot fluorescence coded microsphere and preparation method thereof
  • Quantum dot fluorescence coded microsphere and preparation method thereof
  • Quantum dot fluorescence coded microsphere and preparation method thereof

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preparation example Construction

[0033] see figure 1 As shown, the embodiment of the present invention provides a method for preparing quantum dot fluorescence-encoded microspheres, including:

[0034] S1. Dissolve N kinds of quantum dots of different colors in the mixed solution formed by oil-soluble monomers and crosslinking agents respectively, to obtain N kinds of oil phase mixtures, wherein N≥2;

[0035] S2. Disperse the N oil phase mixtures in the same emulsifier solution in order of the wavelengths of the quantum dots dissolved therein from long to short to form quantum dot micelles;

[0036] S3, adding quantum dot micelles and initiators to water, and performing polymerization under nitrogen protection to obtain quantum dot seed microspheres;

[0037] S4. Adding functional monomers into the polymerization reaction system for grafting reaction, and performing purification treatment after the reaction to obtain quantum dot fluorescence-encoded microspheres.

[0038] It should be noted that although th...

Embodiment 1

[0072] This embodiment provides a preparation method of quantum dot fluorescent microspheres, the specific steps are as follows:

[0073] 1) Mix styrene and ethylene glycol diacrylate at a mass ratio of 5:20 to form a mixed solution, then dissolve ZnCdSe / ZnS quantum dots with emission wavelengths of 625nm and 525nm respectively in the mixed solution, and configure to obtain the mass concentration of quantum dots 20% red quantum dots-styrene / ethylene glycol diacrylate solution (i.e. red oil phase mixture) and 20% green quantum dots-styrene / ethylene glycol diacrylate solution (i.e. green oil phase mixture);

[0074] 2) Weigh 0.2g of sodium dodecylsulfonate, dissolve it in 100mL of water to form emulsion micelles, and then add 4mL of 20% red quantum dot-styrene / ethylene glycol diacrylate solution, which is configured as an emulsifier The mass concentration of the solution is 0.2%, stirred at 20°C for 10min, the red oil phase mixture is uniformly dispersed in the emulsion micelle...

Embodiment 2

[0078] The difference between this embodiment and embodiment 1 is:

[0079] 1), the mass ratio of styrene to ethylene glycol diacrylate in the mixed solution is 1:20, and the mass concentration of quantum dots in the oil phase mixture prepared is 5%;

[0080] 2), the mass concentration of emulsifier is 0.2%, stirring at 0°C for 20min;

[0081] 3), the temperature in the polymerization reaction is 60° C., and the reaction time is 16 hours.

[0082] All the other steps and parameters are the same as in Example 1.

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Abstract

The invention provides a quantum dot fluorescence coded microsphere and a preparation method thereof. The preparation method comprises the following steps: respectively dissolving N kinds of quantum dots with different colors in a mixed solution formed by an oil-soluble monomer and a cross-linking agent to obtain N kinds of oil-phase mixtures, wherein N is greater than or equal to 2; sequentiallydispersing the N oil-phase mixtures into the same emulsifier solution according to the sequence that the wavelengths of the quantum dots dissolved in the N oil-phase mixtures are decreasing so as to form quantum dot micelles; adding the quantum dot micelles and an initiator into water, and carrying out a polymerization reaction under the protection of nitrogen to obtain quantum dot seed microspheres; adding a functional monomer into a polymerization reaction system, carrying out a grafting reaction on the quantum dot seed microspheres and the functional monomer, and carrying out purification treatment after the reaction is finished so as to obtain the quantum dot fluorescence coded microsphere.

Description

technical field [0001] The invention relates to the technical field of nano-biological materials, in particular to a quantum dot fluorescence-encoded microsphere and a preparation method thereof. Background technique [0002] Compared with traditional fluorescent dyes, quantum dots have the excellent properties of wider excitation wavelength range, narrower emission peak and resistance to photobleaching, and are a more ideal fluorescent coding material. However, the shortcomings of quantum dots, such as small particle size, high surface energy, and oxygen resistance, limit their applications. Quantum dot fluorescent microspheres obtained by encapsulating quantum dots in polymer microspheres can significantly improve the optical stability, colloidal stability and biocompatibility of quantum dots, and can also amplify the detected fluorescent signal, significantly improving biological detection. sensitivity. At the same time, quantum dots of different colors can be embedded ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/16C08J7/12C09K11/88C09K11/02C08F222/14C08F212/08C08K3/30
CPCC08J7/16C08J7/12C09K11/883C09K11/025C08F222/102C08K3/30C08K2003/3009C08J2335/02C08F212/08
Inventor 庞代文林乐平朱小波郭三维朱东亮董博然徐越
Owner 广州珈源新材料有限公司
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