The invention relates to the technical field of enzymolysis, in particular to an enzymolysis process which comprises the following steps:
cutting dry
fish skin into pieces, and adding water to obtain rehydrated
fish skin; performing multi-stage enzymolysis and
enzyme inactivation treatment on the rehydrated
fish skin to obtain enzymatic
hydrolysate; adding absolute ethyl
alcohol, a
demulsifier and
sodium chloride into the enzymatic
hydrolysate, stirring for dissolving, and standing for layering to obtain an
ethanol phase and a polypeptide water phase; adding a composite adsorbent into the polypeptide water phase, stirring and adsorbing, then transferring into a plate-and-frame
filter press, and collecting filtrate; the filtrate is pumped into an
ultrafiltration membrane unit, permeate is collected, then the permeate enters a
nanofiltration membrane unit to be desalted and concentrated, a concentrated solution is obtained, the concentrated solution is transferred into a
spray dryer, and the
collagen peptide is obtained. A chelating agent and
citric acid are added in the rehydration stage, on one hand,
metal ions causing fishy smell amplification in the fish
skin are chelated, and peculiar smell substances generated by catalyzing
fat oxidation and
protein degradation are avoided; on the other hand, the acidity of the
system is adjusted,
mucus and impurities on the surface of the fish
skin can be partially dissolved, and peculiar smell sources are further reduced.