430 results about "High protein" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

According to the brittleness baking control process based on the three-section variable-temperature hot air, the hot air flow field and material moisture migration are accurately regulated and controlled, and integrated control over brittleness structure shaping and energy-saving baking of products such as pet chews and jerky is achieved. A hardware architecture surrounds a'sensing-decision-execution 'closed-loop design, each part is tightly coupled with a central controller through an industrial bus, a dual-objective optimization system of brittleness formation and energy consumption control is constructed according to the high-protein and multi-fiber material characteristics of pet food, and the energy consumption of the pet food is controlled on the premise of ensuring that the brittleness index C of the pet food is greater than or equal to 65. Through a heat recovery unit, intermittent countercurrent air supply and LSTM feedforward control, the comprehensive energy consumption is reduced compared with the traditional process, and the unit brittleness energy consumption is reduced.

The invention relates to the technical field of artificial intelligence, and provides a protein function prediction method and device based on multi-modal protein data, and the method comprises the steps: obtaining protein multi-source data, carrying out the feature extraction of a protein sequence in the protein multi-source data, and obtaining a protein sequence feature; constructing a heterogeneous graph based on the protein multi-source data; performing feature coding on the heterogeneous graph by adopting a graph attention mechanism to obtain protein graph features; performing multi-modal fusion on the protein sequence features and the protein map features by adopting a gating fusion mechanism to obtain fusion features; and performing multi-label classification prediction based on the fusion features to obtain a protein function annotation result. The accuracy and robustness of protein function prediction can be improved, and the problems that in the prior art, multi-source protein data cannot be effectively integrated, and the method is sensitive to data noise are solved.

The invention discloses a carbon quantum dot-polyvinyl alcohol composite film and a preparation method thereof, and belongs to the technical field of food packaging fresh-keeping materials. The composite film is prepared by taking apocynum venetum carbon quantum dots and polyvinyl alcohol as raw materials under the action of a plasticizer, wherein the apocynum venetum carbon quantum dots are prepared from apocynum venetum fiber powder, dithiosalicylic acid and urea through a hydrothermal method. The composite film provided by the invention has excellent ultraviolet shielding, pH-responsive fluorescence discoloration, antibacterial and antioxidant properties, and can realize visual monitoring of the freshness of high-protein food. The preparation method disclosed by the invention is simple in process, environment-friendly, suitable for the field of food active packaging and wide in application prospect.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention discloses a high-industrial-property yarrowia lipolytica strain and application thereof, and belongs to the technical field of microbial engineering and fermentation. The mutant is derived from a natural bacterial strain of fermented vegetables, is obtained through high-throughput cell screening, has the advantages and characteristics of high salt resistance, low pH resistance, efficient utilization of an acetic acid substrate, high protein content, no need of vitamins for growth and the like, and is suitable for industrial production of microorganisms; the method can support efficient production of bio-manufactured products under strict conditions of acetic acid carbon source, no sterilization, no seawater, no vitamin and the like.

The invention relates to a protein solubility prediction method and system based on GCN and an improved attention network. The method comprises the following steps: constructing a protein sequence data set; the method comprises the following steps: acquiring a protein three-dimensional structure file through AlphaFold3, and constructing a node feature vector; extracting position embedding, bidirectional projection direction features, a cross-node atom pair distance and a rotation relation, and constructing an edge feature vector; extracting global physicochemical property feature vectors of the protein; and constructing protein graph structure data, inputting the data to the graph convolutional network module and the improved graph attention network module, and performing training to obtain a graph convolutional network and an improved attention network model to perform protein solubility prediction. By extracting node and edge features and protein global physicochemical property features, subsequent model training is facilitated; and the two-way projection and the rotation relation enable the model to accurately sense the spatial orientation between residues, so that the model has more quantitative understanding on the protein folding and gathering driving force, and the accuracy of protein solubility prediction is improved.

The present disclosure relates to a process for obtaining sunflower protein isolates which encompasses the steps of shelling, cold pre-pressing, oil extraction, protein extraction and isolate purification. The process optimizes protein yield while employing mild processing conditions to maintain functionality. The obtained isolates exhibit high protein content, desirable emulsification capacity and water / oil retention, and neutral color characteristics, making them suitable for a variety of food applications. Thus, a sunflower globulin protein isolate, a sunflower albumin protein isolate and the use of the sunflower globulin protein isolate or sunflower albumin protein isolate in food products are also disclosed.

To provide konjak-like food with high protein content, while utilizing the advantages of konjac, which has a unique texture and a low caloric value.SOLUTION: A liquid mixture contains 0.3 w / w% or more and 2.5 w / w% or less of glucomannan, 0.2 w / w% or more and 2.5 w / w% or less of alginic acid and / or a salt thereof, and 3.0 w / w% or more and 12.0 w / w% or less of vegetable protein, optionally containing 0.1 w / w% or more and 1.5 w / w% or less of xanthan gum, and substantially free of gluten. The liquid mixture is discharged into an aqueous solution containing divalent cations to produce a gel-like product.SELECTED DRAWING: None

The utility model relates to cottonseed high protein production and processing technical field, especially a kind of cottonseed high protein production shell in cotton kernel recycling device. The device contains bending shell (1), bending shell (1) top is air port connecting flange (8), bending shell (1) top contains a rectangular feed slot (7) of inclined arrangement;The mouth on rectangular feed slot (7) can enter material;Air port connecting flange (8) can connect suction force providing structure. Advantageous effects: by the above technical scheme, the utility model can effectively reduce the content of small cottonseed and cotton kernel in cotton shell, improve the quality and yield of cottonseed white egg, reduce resource waste and improve enterprise economic efficiency.

The present disclosure provides methods and compositions for producing, detecting, and selecting soybean plants and seeds comprising at least one high protein CCT (CONSTANS, CO-like and TOC1) domain containing variant allele and introgressing the high protein CCT variant allele into soybean plants. The present disclosure also provides methods and compositions for producing, detecting, and selecting soybean plants producing seeds having a high protein content including breeding methods for introgressing high protein alleles into soybean plants using marker assisted selection using markers linked to or associated with high protein CCT in soybean.

The application discloses application of a corn gene in improving corn kernel protein content, and relates to the technical field of biology. ZmAAP16 A new gene capable of improving corn kernel content is mined by the application. ZmAAP16 Through transgenic method, function of the gene is analyzed and identified in depth, it is found that the gene can significantly improve the corn kernel protein content by overexpression in plants, and the role of the gene in improving the corn kernel protein content is determined. The application has important significance for cultivation of high-protein ensiled corn.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

The invention discloses a method for synergistically improving the bleeding problem in the storage process of pea starch-quinoa protein gel through proteolysis and gellan gum addition. The invention provides a method for preparing gel. The method comprises the following steps: (1) mixing enzymolysis quinoa protein, pea starch, gellan gum and water to obtain a mixed suspension; and (2) heating and gelatinizing the mixed suspension, and cooling and molding to obtain the gel. The gel prepared by the method has the beneficial effects that the water binding capacity is high, and the bleeding rate is low; microscopic structure observation shows that a highly compact and uniform network is formed, and no obvious bubbles exist; low-field nuclear magnetic resonance analysis shows that the bound water proportion is obviously increased; a dynamic rheological test proves that the gel has excellent gel stability; and the process is simple and safe and easy for industrial production. The invention provides a reliable technical scheme for the development of high-protein nutritional soft food for old people.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

The present disclosure relates to a method for manufacturing a molded article in which the molding ability of a protein is improved. Also provided is a composition for molding consisting of at least a mixture of a protein and a pyrolytic water discharge substance is compressed to produce a molded article.

A method and system for increasing the purity of protein from a system that produces protein, such as a high protein meal, from dry milling of grains including, for example, corn and wheat. The purity of the high protein meal, for example, can be increased by back end enzyme treatment of yeast, which has been separated along with protein from whole stillage. The enzyme(s) can breakdown the yeast into its yeast components that can be removed from the protein to provide for an increased purity of a resulting high protein product.

The objective of the present invention is to provide a method for manufacturing powdered cultured meat that can increase cell differentiation efficiency and protein content with economic feasibility, and to provide powdered cultured meat with high physicochemical stability and transportability, as well as a food composition containing the same. The powdered cultured meat has a flavor similar to real meat and, as a high-protein food, has excellent utility in various food compositions or processed foods.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The utility model discloses baking equipment for processing coarse cereal high-protein baked products, which comprises a device body, a baking box is arranged in the device body, an operating table is arranged on the baking box, a mounting groove is formed in the baking box, an adjusting knob, a switch knob, a temperature display screen and an operating button are sequentially arranged on the operating table, and the operating button is arranged in the mounting groove. A controller and a mounting frame are arranged in the baking box, the controller is connected with a heating pipe, a placing plate is arranged on the inner wall of the mounting frame, a first baking tray, a second baking tray, a third baking tray, a fourth baking tray and a collecting box are sequentially arranged on the placing plate, a lead screw is arranged in a mounting groove, a lantern ring is connected to the lead screw in a threaded mode, and one end of the lead screw is connected with a motor. A user can conveniently place products needing to be baked, the situation that the hands stretch into a high-temperature area to be scalded is avoided, the user can conveniently clean the baking tray in the later period, dead corners are reduced, the baking quality of the products is guaranteed, and the flexibility of the device is improved.

This invention pertains to the purification of microbial fermentation broth, specifically a method for purifying fermentation broth containing β-1,3 / α-1,3-glucan. The method involves ethanol extraction and filtration to extract β-1,3 / α-1,3-glucan, including fermentation broth pretreatment, pH adjustment of the clarified broth after filtration, at least two ethanol extractions and filtrations, and drying and pulverizing the resulting fibrous material to obtain β-1,3 / α-1,3-glucan. This invention solves the problems of low extraction rate and low product purity in existing technologies, offering advantages such as high extraction rate and product yield, low protein and impurity content, and low production cost.

The invention discloses a method and a device for stimulating growth of oat seeds by steam jet negative corona discharge, after steam passes through an electric field, the oat seeds are stimulated by the steam jet negative corona discharge, the discharge intensity is controlled by selecting different discharge time, the oat seeds are treated, and the treated seeds are planted. The germination, growth and harvesting processes of the oat seeds are studied, and changes of components, structures and functional characteristics of mature oat powder are discussed; results show that the low-intensity (5 min) treatment significantly improves the crude protein content of the oats, reduces the total starch content, promotes the photosynthesis and plant growth of oat plants, increases the number of grains, increases the ordering degree and stability of the oat powder, and is beneficial to forming a colloid system which is rich in water and stable in structure. The invention proves that an oat product with high protein and low starch content can be obtained through proper steam jet negative corona discharge treatment, the microstructure is more ordered, and the gelatinization performance of the oat can be obviously improved.

According to the method for making the walnut meal noodles, the protein content of the noodles is increased by adding the walnut meal, meanwhile, the problem of processing performance degradation is solved by utilizing the synergistic effect of curdlan, edible salt and water, the nutritional ingredients in the walnut meal are reserved through a low-temperature drying technology, and balance of nutrition and quality is achieved. According to the method, the nutritional quality of the noodles can be remarkably improved, the protein content of the prepared walnut meal noodles reaches 13.34%, the high-protein dietary standard (larger than or equal to 13%) is met, the cooking loss rate is smaller than or equal to 7.5%, the elasticity is larger than or equal to 0.95 mm, the cost can be reduced, walnut meal waste recycling is achieved, the technology compatibility is high, and the practical popularization value is achieved.

The present invention provides a high-protein bio-fermented aquatic feed and a preparation method thereof. The aquatic feed comprises the following raw materials in parts by weight: 20-30 parts of imported fish meal, 10-15 parts of compound minerals, 5-10 parts of bioactive peptides, 5-10 parts of lysine, 5-10 parts of compound trace elements, 3-8 parts of compound vitamins, 3-6 parts of herbal extracts, 2-5 parts of compound probiotics, 2-4 parts of immune polysaccharides, and 1-3 parts of compound enzymes; the herbal extracts are prepared by fermenting astragalus membranaceus, isatis root, liquorice root, calotropis gigantea, ventilago leiocarpa benth, clove, rhizoma chuanxiong, and galangal officinale. By organically integrating imported fish meal, astaxanthin-peptide chelate, herbal extracts, compound immune polysaccharides, compound probiotics and various nutrients, the present invention realizes the synergistic effect of "promoting feeding - enhancing digestion - strengthening liver and gall - enhancing immunity - resisting stress - inhibiting bacteria - stabilizing water quality", significantly improves the growth rate, survival rate and comprehensive breeding benefit of crustacean aquatic animals, and has good economic value and application prospect.

A high-purity yeast protein and a preparation method therefor, wherein the method comprises the following steps: inoculating an activated yeast strain into a liquid seed culture medium, culturing same in a shaker, then inoculating same into a fermentation culture medium for culturing, and centrifuging and washing same to obtain a yeast strain; preparing the yeast strain into a suspension solution, and performing a heat treatment to collect a precipitate; preparing the precipitate into a suspension, adding 5-50 U / mL of dextranase and 5-50 U / mL of cellulase at a pH of 5.0-7.0 and 30-60°C, and performing enzymatic hydrolysis to obtain a first enzymatic hydrolysate; adding a non-ionic solubilizer to the first enzymatic hydrolysate at a certain pH, and incubating the resulting mixure at a certain temperature to obtain a second enzymatic hydrolysate; and subjecting the second enzymatic hydrolysate to solid-liquid separation, washing and drying to obtain a high-purity yeast protein. The method reduces the possibility of protein denaturation. The final yeast protein product has a low dietary fiber content, a high branched-chain amino acid content and a high protein content, and exhibits good nutritional value and processing characteristics.

The invention relates to a protein composite fiber after-finishing method and application thereof. The preparation method comprises the following steps: firstly, carrying out pretreatment such as cleaning and drying on protein composite fibers, and then mixing the protein composite fibers with a tannin-containing cross-linking solution to carry out primary cross-linking so as to construct a stable cross-linked network; soaking the protein composite fiber in a metal ion solution for secondary coordination crosslinking, and sequentially performing oiling, high-temperature setting and the like to finally obtain a protein composite fiber finished product with excellent heat resistance, mechanical property and high protein retention rate. The problems that fibrous protein components are prone to loss, traditional aldehyde or metal phosphorus cross-linking agents are toxic and harmful and the like are solved, the comprehensive performance of the protein composite fiber is remarkably improved through a specific cross-linking reaction between polyphenol structures in tannin of different sources and active groups of the fiber and a chelation coordination reaction of metal ions, and the protein composite fiber has the advantages of being high in practicability and wide in application prospect. The method can be applied to multiple fields such as high-end spinning and medical hygienic materials.

The invention discloses a strong cation exchange chromatography medium as well as a preparation method and application thereof, and belongs to the technical field of biological separation and purification. The structural formula of the strong cation exchange chromatography medium is shown in the specification, the preparation method comprises the following steps: (1) synthesizing epoxy porous polymer microspheres; (2) carrying out an epoxy-sulfydryl click reaction with sulfydryl sulfonate; and (3) hydrolyzing the residual epoxy group to obtain the epoxy resin. The strong cation exchange chromatography medium has the advantages of high protein loading capacity, low non-specific adsorption, good mechanical property and the like, the preparation process is simple, special hydrophilic modification is not needed, and the process is simplified, the industrial production period is shortened and the production cost is reduced under the condition of meeting the conventional protein elution condition. And meanwhile, the high efficiency of the click reaction can meet the characteristics of high loading capacity, good biocompatibility and the like of the chromatography medium.

The present invention provides a universal protein renaturation method and an application thereof, and relates to the technical field of protein renaturation. The method includes the following steps: (1) add 2mercaptoethanol to urea solution to obtain denaturing solution; (2) dissolve a sample including to-be-renatured protein by using the denaturing solution and then freeze the sample, to obtain a frozen body; (3) thaw the frozen body at room temperature, and extract the thawed liquid as soon as the liquid is thawed; (4) dilute the extracted liquid with water, to terminate renaturation and obtain supernatant and precipitate through centrifugation, where the supernatant includes the protein that has been successfully renatured. According to the present invention, protein renaturation recovery efficiency can be improved, and a production batch is stable and reproducible. Operations in the present invention are simple, almost no waste liquid is produced, and no protein is lost. According to the present invention, nine allergen proteins are renatured, to significantly improve the protein renaturation yield. In addition, compared with that in the conventional technology, the protein activity according to the present invention is increased by about 3 times or more.

The invention relates to the technical field of packaging materials, in particular to an intelligent packaging film based on a multilayer packaging nanocomposite and a preparation method of the intelligent packaging film. On the basis of the anthocyanin CD-MOFs (core) with a physical adsorption function, an ovalbumin-sodium alginate (OVA-SA) nano-composite is innovatively introduced to construct a core-shell structure, and an OVA-SA shell and a CD-MOFs pore channel form a double barrier, so that the stability of the anthocyanin is remarkably improved; the OVA-SA shell is used as an interfacial compatibilizer, and the high chemical affinity of the OVA-SA shell and a corn starch / PVA matrix is utilized, so that nano-scale uniform dispersion of MOF particles in the matrix is realized, and agglomeration is effectively avoided; meanwhile, an active pH response gated release mechanism is formed, high stability is kept in the shelf life, shell response is triggered only when a decay product induces pH rise, anthocyanin is released, color development is carried out, and sensitive monitoring on the freshness of the high-protein food is achieved.

The invention relates to a magnetic bead mediated protein purification device and a high-purity extraction method, and belongs to the technical field of protein purification. The magnetic bead mediated protein purification device comprises a bottom seat, the upper end of the bottom seat is fixedly connected with a circular shell, and the inner bottom of the circular shell is fixedly connected with a bottom magnet; the purification mechanism is mounted on the inner bottom wall of the circular shell; wherein the purification mechanism comprises a plurality of magnetization assemblies mounted on the inner wall of the bottom seat; according to the magnetic bead mediated protein purification device and the high-purity extraction method, the device drives the liquid and the magnetic beads to be mixed through the arranged magnetizing assembly, and after mixing is completed, relatively uniform magnetic attraction can be formed on the liquid storage pipe through the magnetizing assembly, so that the magnetic beads can be uniformly distributed on the inner wall of the liquid storage pipe, and the magnetic beads are uniformly separated from the liquid storage pipe; the occurrence of magnetic bead breakage is reduced, and the extraction effect of protein purification is improved.