12 results about "Tyramine" patented technology

The present application relates to the field of bioengineering, and particularly to a mutant of an enzyme, a mutant of a glycosyltransferase and application thereof in preparing rhodiolin. The present application provides a mutant of an enzyme, which has the following amino acid site mutations on the basis of a wild-type glycosyltransferase: the 308th site is mutated from I to Q, the 333rd site is mutated from V to R, the 363rd site is mutated from W to S and the 386th site is mutated from S to G, and the amino acid sequence of the wild-type glycosyltransferase is shown as SEQ ID NO: 1. The present application obtains a glycosyltransferase and a sucrose synthase with improved thermal stability through structural rational design and deep learning modification, and can glycosylate tyramine to generate rhodiolin in the form of regenerating ADP-glucose, and the price of ADP is only about 20% of UDP, which has a cost advantage.

The invention provides a phenylpyruvate decarboxylase mutant with multi-site mutation and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The problem of low yield of tyrosol is solved. The phenylpyruvate decarboxylase mutant comprises a phenylpyruvate decarboxylase mutant, and the phenylpyruvate decarboxylase mutant is obtained by mutating any two or three sites of the 93rd site, the 106th site, the 153rd site, the 220th site, the 242nd site, the 273rd site and the 285th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The method is mainly used for increasing the yield of tyrosol.

The invention discloses a composite hydrogel capable of delaying retention of plasma active substances and a preparation method and application thereof, and the preparation method comprises the following steps: inducing a hyaluronic acid-tyramine solution through CAP to form hydrogel, so that active oxygen and active nitrogen are preserved in the hydrogel, a nano material is added to preserve more active substances, and the stability is improved. The prepared hydrogel does not lose efficacy within one month, the actual action time of CAP is prolonged to a great extent, traumatic surgery is overcome and can be replaced, and skin squamous cell carcinoma is treated through minimally invasive injection.

The application discloses a salt-tolerant flavor-enhancing biological amine degradation strain Staphylococcus carnosus WQ-4 and application thereof, and belongs to the technical field of biological fermentation. The Staphylococcus carnosus WQ-4 has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251802. The strain has good salt tolerance and biological amine degradation capacity, has obvious degradation effect on histamine and tyramine which are common biological amines in fermented food, the degradation rates are 55.12% and 81.47% respectively, and has good stability, safety and flavor-enhancing effect in a soy sauce fermentation environment, and can be used for soy sauce brewing.

The invention discloses a method for reducing the content of biogenic amines in meat products through composite vacuum rapid pickling, and belongs to the technical field of food processing. According to the method disclosed by the invention, the pork tenderloin, the chicken breast and the beef tendon are pickled by adopting the pickling liquid prepared by compounding the anthocyanin and the lipopeptide and assisting ultrasonic and vacuum modes, so that the pickling time of the meat product is shortened, the quality of the meat product is improved, the generation of biogenic amine is also inhibited, histamine and tyramine are not detected in the pork tenderloin pickled by the method, and the pork tenderloin is free of toxic and side effects. The content of cadaverine and spermidine is remarkably reduced, and the product is uniform in meat quality, excellent in flavor and high in safety. The method provides a theoretical basis for controlling the safety of meat products and optimizing the processing technology while improving the meat pickling efficiency, and has a key guiding significance for developing low-salt and low-biogenic-amine meat products.

The invention discloses a phenylpyruvate decarboxylase mutant and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The purpose of the present invention is to increase the yield of tyrosol. The phenylpyruvate decarboxylase mutant provided by the invention is obtained by mutating any one of the 56th site, the 92th site, the 93th site, the 106th site, the 115th site, the 153th site, the 178th site, the 188th site, the 214th site, the 220th site, the 234th site, the 242th site, the 273th site, the 285th site, the 371th site, the 416th site, the 581th site and the 621th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The phenylpyruvate decarboxylase is mainly used for high-efficiency phenylpyruvate decarboxylase and application thereof in de novo synthesis of tyrosol.

The present application relates to the field of agricultural cultivation technology, and more particularly to a method for improving the low-temperature stress tolerance of cherry tomato seedlings by exogenous tyramine. The method selects cherry tomato seedlings at the 4-leaf 1-heart stage, and sprays the cherry tomato seedlings in the experimental group with a tyramine water solution at a concentration of 1-2.5 mg / L at 5 pm every day. The spraying is performed according to the standard of covering the leaves with mist droplets and no dripping. The cherry tomato seedlings in the normal temperature and low temperature control groups are sprayed with distilled water. The influence of tyramine on cherry tomatoes under low-temperature stress is observed. The test results prove that exogenous tyramine significantly alleviates the growth of cherry tomatoes under low-temperature stress, reduces the MDA and H2O2 contents in the leaves of cherry tomatoes, increases the proline and soluble sugar contents, and enhances the activity of antioxidant enzymes, thereby effectively reducing the damage of low-temperature stress to cherry tomato plants.

This invention discloses a strain of *Lactobacillus sarcodactylis* with stable biogenic amine degradation ability and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of *Lactobacillus sarcodactylis* L18 from fermented soybean paste, capable of degrading biogenic amines. This strain can simultaneously degrade eight common biogenic amines: tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine. The *Lactobacillus sarcodactylis* L18 screened in this invention exhibits rapid growth, strong environmental adaptability, and stable amine-reducing performance. It can continuously exert its biogenic amine degradation effect under low to medium-high salt, acidic to weakly alkaline conditions, and common fermentation temperature conditions, making it particularly suitable for the effective control of biogenic amines such as tyramine in fermented foods.

The invention relates to the field of biological agents, particularly discloses an immune color developing reagent for detecting malignant tumors in urine and application of the immune color developing reagent, and is used for solving the problems that in bladder cancer urine exfoliated cell detection, an acridine orange single staining color developing image is poor in bladder tumor specific epitope recognition, heavy in background interference and high in spectrum overlapping and manual interpretation subjectivity. The color developing reagent comprises a reagent A, a reagent B, a reagent C and a reagent D. The reagent A is provided with an antibody complex and acridine orange aiming at tumor cell target antigens of bladder parts in urine shedding, and the reagent B comprises a fluorescently-labeled secondary antibody composition and an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent A, and the reagent D is provided with an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent D. In the application, AI imaging analysis is adopted, and layered identification labels and acridine orange dyeing negative and positive labels are calibrated; through multi-target immune color development, two sets of color separation amplification schemes and AI imaging analysis, the bladder tumor specific epitope recognition capability, the image quality and the image analysis accuracy of urine cast-off cell detection are improved.

The invention relates to the technical field of biological medicine, and provides a pharmaceutical composition containing alzvudine and a VEGFR-TKI inhibitor and application of the pharmaceutical composition in resisting liver cancer. In-vivo and in-vitro experiments prove that the alzvudine and the VEGFR-TKI have an obvious synergistic effect in liver cancer treatment, and the pharmaceutical composition provided by the invention can still ensure and improve the anti-tumor curative effect on the basis of obviously reducing the clinical use dosage of the core targeted drug VEGFR-TKI, and has a good application prospect. The pharmaceutical composition provided by the invention can effectively reduce the incidence rate of core targeted drug-related adverse reactions, has good safety, has significant advantages in the aspects of improving the treatment compliance and life quality of patients, and provides a new strategy for solving the clinical problem that the curative effect and toxicity in targeted treatment of liver cancer are difficult to consider at the same time.

The invention discloses an amido bond synthetase mutant with improved stability and application of the amido bond synthetase mutant. The amino acid sequences of the amido bond synthetase mutant are SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11. Compared with wild type zymoprotein, the half-life period of the mutant constructed by the invention can be prolonged to 1.54 times of the original half-life period under the condition of the optimal reaction temperature. The mutant constructed by the invention uses ferulic acid as a substrate to catalytically produce feruloyl tyramine, the highest conversion rate can reach 100%, and the production cost can be greatly reduced.

This invention discloses a visual detection method and application of biogenic amines based on a carbon dot / quantum dot ratio fluorescent probe, belonging to the field of food safety detection technology. Addressing the problems of existing biogenic amine detection methods, such as complex operation, difficulty in rapid on-site screening, and susceptibility to interference from single fluorescent signals, this invention prepares a ratio fluorescent probe based on graphene carbon dots (G-CDs) and near-infrared quantum dots (QD700). This probe requires no chemical modification and achieves a synergistic response of G-CDs fluorescence enhancement and QD700 fluorescence quenching through the Spe-induced aggregation effect, achieving a detection limit of 0.0097 μM and a response time faster than 2 seconds. Combined with the DEIM intelligent model, it can accurately distinguish spermine, putrescine, cadaverine, histamine, and tyramine. Integrated into a paper-based smartphone platform, it enables visual identification of shrimp freshness (red for fresh, green for spoiled), showing a high correlation with the TVB-N standard method (R²=0.95), suitable for rapid on-site monitoring.