27 results about "Tyramine" patented technology

The present application relates to the field of bioengineering, and particularly to a mutant of an enzyme, a mutant of a glycosyltransferase and application thereof in preparing rhodiolin. The present application provides a mutant of an enzyme, which has the following amino acid site mutations on the basis of a wild-type glycosyltransferase: the 308th site is mutated from I to Q, the 333rd site is mutated from V to R, the 363rd site is mutated from W to S and the 386th site is mutated from S to G, and the amino acid sequence of the wild-type glycosyltransferase is shown as SEQ ID NO: 1. The present application obtains a glycosyltransferase and a sucrose synthase with improved thermal stability through structural rational design and deep learning modification, and can glycosylate tyramine to generate rhodiolin in the form of regenerating ADP-glucose, and the price of ADP is only about 20% of UDP, which has a cost advantage.

The invention provides a phenylpyruvate decarboxylase mutant with multi-site mutation and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The problem of low yield of tyrosol is solved. The phenylpyruvate decarboxylase mutant comprises a phenylpyruvate decarboxylase mutant, and the phenylpyruvate decarboxylase mutant is obtained by mutating any two or three sites of the 93rd site, the 106th site, the 153rd site, the 220th site, the 242nd site, the 273rd site and the 285th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The method is mainly used for increasing the yield of tyrosol.

The invention relates to the field of microorganisms, and provides a plant lactobacillus FVPGZP2101 and application thereof, the plant lactobacillus FVPGZP2101 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32871. The invention also provides a preparation method of the plant lactobacillus FVPGZP2101. The new strain of the plant lactobacillus obtained by screening has the advantages of strong growth ability, high acid production efficiency, strong acid resistance, capability of degrading nitrite, tryptamine, tyramine, spermidine and spermine, no generation of biogenic amine, no degradation of pectin, antibacterial activity on pathogenic bacteria, oxidation resistance of extracellular metabolites, and no pollution to the environment. According to the present invention, the new strain has advantages of high yield, key volatile substance production and non-volatile substance production, and the fermented food prepared by using the new strain has characteristics of low salt content, rich lactic acid bacteria content, nutrition, health and unique flavor; a food fermentation process developed by utilizing the new strain disclosed by the invention is high in fermentation efficiency, short in production period and high in application value.

The invention discloses a salt-tolerant Bacillus velezensis strain capable of efficiently degrading protein and biogenic amine and an application of the salt-tolerant Bacillus velezensis strain. The bacillus velezensis is a strain FJNU-SIO1, is classified and named as bacillus velezensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is September 26, 2025, the preservation number is CGMCC No.36086, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The strain FJNU-SIO1 is wide in growth adaptation range, can tolerate the salt concentration of 1%-10%, and can still efficiently degrade protein and nine common biogenic amines including putrescine, cadaverine, histamine and tyramine under the high salt concentration of 10%, the degradation rate of the protein can reach 47.68%, and the degradation rate of the total biogenic amines can reach 32.77%. The strain FJNU-SIO1 provided by the invention has the outstanding advantages of simplicity and convenience in operation, low cost, wide applicability and the like when being applied to degradation treatment of proteins and biogenic amines, and has an important value for guaranteeing safe production of fermented foods.

The invention discloses a composite hydrogel capable of delaying retention of plasma active substances and a preparation method and application thereof, and the preparation method comprises the following steps: inducing a hyaluronic acid-tyramine solution through CAP to form hydrogel, so that active oxygen and active nitrogen are preserved in the hydrogel, a nano material is added to preserve more active substances, and the stability is improved. The prepared hydrogel does not lose efficacy within one month, the actual action time of CAP is prolonged to a great extent, traumatic surgery is overcome and can be replaced, and skin squamous cell carcinoma is treated through minimally invasive injection.

The application discloses a salt-tolerant flavor-enhancing biological amine degradation strain Staphylococcus carnosus WQ-4 and application thereof, and belongs to the technical field of biological fermentation. The Staphylococcus carnosus WQ-4 has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251802. The strain has good salt tolerance and biological amine degradation capacity, has obvious degradation effect on histamine and tyramine which are common biological amines in fermented food, the degradation rates are 55.12% and 81.47% respectively, and has good stability, safety and flavor-enhancing effect in a soy sauce fermentation environment, and can be used for soy sauce brewing.

The application belongs to the technical field of crop planting, and discloses application of N-cis feruloyl tyramine in improving alkali stress tolerance of rice. ‑8 ~10 ‑4 g / L of the working solution, and spraying the leaves at the one-leaf-one-heart stage of the rice seedlings. The application can significantly improve the chlorophyll content of the rice under alkali stress environment, alleviate chlorophyll degradation, promote the growth of the aboveground part, and effectively enhance the alkali stress tolerance of the rice at the seedling stage through simple foliar spraying operation. The method is simple in operation, low in cost, high in environmental protection and efficiency, and has important agricultural application value for stable yield and yield increase of rice in saline-alkali land and utilization of marginal land resources.

The invention discloses a method for reducing the content of biogenic amines in meat products through composite vacuum rapid pickling, and belongs to the technical field of food processing. According to the method disclosed by the invention, the pork tenderloin, the chicken breast and the beef tendon are pickled by adopting the pickling liquid prepared by compounding the anthocyanin and the lipopeptide and assisting ultrasonic and vacuum modes, so that the pickling time of the meat product is shortened, the quality of the meat product is improved, the generation of biogenic amine is also inhibited, histamine and tyramine are not detected in the pork tenderloin pickled by the method, and the pork tenderloin is free of toxic and side effects. The content of cadaverine and spermidine is remarkably reduced, and the product is uniform in meat quality, excellent in flavor and high in safety. The method provides a theoretical basis for controlling the safety of meat products and optimizing the processing technology while improving the meat pickling efficiency, and has a key guiding significance for developing low-salt and low-biogenic-amine meat products.

The invention is suitable for the technical field of bio-based high polymer materials, and provides a flame-retardant bio-based epoxy resin monomer, bio-based epoxy resin and a preparation method thereof. The flame-retardant bio-based epoxy resin monomer is prepared by taking vanillin and tyramine as raw materials, introducing a DOPO flame retardant through a condensation reaction to generate a bisphenol monomer, and then performing epoxidation and ring-closure reaction. And mixing the epoxy resin with an amino-containing curing agent, and carrying out first curing and second curing to prepare the bio-based epoxy resin. The flame-retardant bio-based epoxy resin monomer provided by the invention has a rigid benzene ring and a phosphorus-containing structure, so that the thermal stability and the mechanical property of epoxy resin can be improved, the flame-retardant bio-based epoxy resin monomer and nitrogen element can be synergistically flame-retardant, a compact and continuous expanded carbon layer is formed during combustion, and the flame-retardant bio-based epoxy resin monomer shows excellent flame retardance. The monomer is prepared from renewable bio-based raw materials vanillin and tyramine, and the process steps are simple, green and environment-friendly. The prepared bio-based epoxy resin is excellent in comprehensive performance, can reduce dependence on petroleum resources, and meets the requirement of green development.

The application belongs to the technical field of bio-based polymer materials, and provides a flame-retardant bio-based epoxy resin monomer, a bio-based epoxy resin and a preparation method thereof.The flame-retardant bio-based epoxy resin monomer is prepared from vanillin and tyramine as raw materials, through a condensation reaction to introduce a DOPO flame retardant to generate a bisphenol monomer, and then through epoxidation and ring closure reaction.The bio-based epoxy resin can be prepared by mixing the monomer with an amino-containing curing agent and then performing first curing and second curing.The flame-retardant bio-based epoxy resin monomer provided by the application has a rigid benzene ring and a phosphorus-containing structure, which can not only improve the thermal stability and mechanical properties of the epoxy resin, but also synergistically flame-retard with nitrogen elements to form a dense and continuous expanded carbon layer during combustion, thereby exhibiting excellent flame retardancy.The monomer is prepared from renewable bio-based raw materials vanillin and tyramine, and the process steps are simple, green and environmentally friendly.The bio-based epoxy resin prepared has excellent comprehensive performance, can reduce the dependence on petroleum resources, and meets the demand for green development.

The invention relates to a bio-based benzoxazine monomer as well as a preparation method and application thereof, and the bio-based benzoxazine monomer is obtained by reaction of 5, 5 '-methylene difurfuryl amine, vanillyl alcohol and paraformaldehyde. The content ratio of the monomer biomass is high, and the resin obtained by melting and curing the monomer biomass is good in heat resistance, high in strength and good in toughness. More importantly, the difference value between the melting temperature and the curing temperature of the monomer is large, the monomer can be copolymerized with a Schiff base phenolic monomer obtained by reacting vanillin with tyramine to prepare the degradable benzoxazine copolymer resin with high biomass content, and the copolymer has outstanding heat resistance, flame retardance, mechanical properties and the like, and is suitable for industrial production. The problems of low biomass content and low heat resistance of degradable biomass benzoxazine resin in the prior art are solved, and the degradable biomass benzoxazine resin has good application prospects in the fields of adhesives, coatings and the like.

The invention relates to the field of pickled Chinese cabbage fermentation, and discloses a secondary fermentation method for improving the flavor of pickled Chinese cabbages, and the method comprises the following steps: salting leaf mustard, adding water, sealing, and carrying out primary segmented fermentation; taking out the pickled Chinese cabbages, desalting and cutting the pickled Chinese cabbages into broken sections; mixing and sealing the crushed pickled Chinese cabbages, a flavor stabilizer and a complex microbial inoculant prepared from lactobacillus plantarum, fused Weissella and ksajas yeast, then carrying out secondary segmented fermentation, and carrying out post-treatment to obtain the pickled Chinese cabbage product. Through the synergistic effect of the lactobacillus plantarum and the ksajas yeast, the contents of key flavor substances such as ethyl acetate and ethyl butyrate in the pickled Chinese cabbages are remarkably increased; nitrites are degraded through fusion of Weissella in secondary segmented fermentation, and the nitrite content of the pickled Chinese cabbages is greatly reduced; meanwhile, the complex microbial inoculant can effectively inhibit growth of spoilage microorganisms, so that harmful ingredients such as histamine and tyramine are remarkably reduced, and the quality and safety of the pickled Chinese cabbages are further improved.

The invention discloses biomass epoxy resin capable of being recycled in a closed loop mode and a preparation method and application thereof. The preparation method comprises the following steps: reacting a biomass material tyramine with protocatechualdehyde to obtain an intermediate; carrying out heating reaction on the intermediate and epoxy chloropropane to obtain a biomass epoxy compound; a biomass epoxy compound and biomass maleic anhydride are mixed and cured to obtain the biomass epoxy resin capable of being recycled in a closed loop mode, and the biomass carbon content of the biomass epoxy resin is 100%. The biomass epoxy resin prepared by the invention not only has excellent heat resistance, but also is easy to recycle, and can be completely degraded in ethylene glycol and realize closed-loop recovery. The problems that biomass epoxy resin is low in biomass carbon content, low in heat resistance, difficult to recycle and the like are solved, a new thought and a new method are provided for promoting green and sustainable development of the heat-resistant biomass epoxy resin, and the method can be applied to the high-performance fields such as aerospace and the like.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing tyrosol as well as a construction method and application of the recombinant corynebacterium glutamicum. The construction method of the recombinant corynebacterium glutamicum for producing the tyrosol comprises the following steps: knocking out a pheA gene of a competitive metabolic pathway, simultaneously carrying out heterologous expression on ARO10 and ADH6 genes, breaking through a tyrosol synthesis pathway, and introducing promoters PNcgl0824, Psod and Peftu with different intensities to enhance the expression of the genes (ARO10 and ADH6), so as to obtain the recombinant corynebacterium glutamicum with increased tyrosol yield. The tyrosol yield of the recombinant corynebacterium glutamicum can be up to 4.41 g / L.

The invention discloses preparation and application of oxygen-triggered gelation spray for diabetes wound treatment, and belongs to the technical field of functional materials. The invention provides an oxygen-triggered gelation hydrogel system containing hirudin and a preparation method of the oxygen-triggered gelation hydrogel system. The hydrogel is prepared from tyramine modified hyaluronic acid (HA-Tyr), glucose oxidase (GOD), horse radish peroxidase (HRP) and glucose (Glu). The components of the hydrogel are kept in a solution state in the spray bottle (after oxygen is removed). After the solution is sprayed, glucose oxidase reacts with glucose in the presence of oxygen to generate hydrogen peroxide (H2O2). The generated H2O2 triggers horse radish peroxidase to catalyze HA-Tyr crosslinking, and the hydrogel is formed. The hydrogel is applied through spraying, is more tightly attached to the surface of a wound, can better play the effects of resisting bacteria, improving microcirculation and reducing the inflammation level, and has remarkable potential in the aspect of treating the chronic diabetes mellitus wound.

The invention relates to the technical field of biology, in particular to a phenylpyruvate decarboxylase ARO10 protein mutant and application of the phenylpyruvate decarboxylase ARO10 protein mutant in preparation of tyrosol. The invention discloses an ARO10 mutant. The phenylpyruvate decarboxylase mutant is obtained by mutating one or more of the 335th site of phenylpyruvate decarboxylase ARO10 (an amino acid sequence shown as SEQ ID NO1) from yeast. Experiments prove that the mutated phenylpyruvate decarboxylase protein can improve the specific reaction on 4-hydroxyphenylpyruvic acid, so that the tyrosol generation capability of saccharomyces cerevisiae is improved. Glycosyl transferase ARO10 mutant protein is expressed in microorganisms, the capacity of the microorganisms for converting tyrosol is obviously enhanced, and the industrial application prospect is good.

The invention discloses a method for fermenting smelly mandarin fish by using potassium chloride to replace salt and inoculate bacteria, and belongs to the technical field of food fermentation processing. Potassium chloride is used for replacing part of sodium chloride, and the content of sodium chloride in the smelly mandarin fish can be remarkably reduced. Potassium chloride is added to replace sodium chloride, the metabolic activity of lactic acid bacteria in the inoculation fermentation process is effectively adjusted, the growth activity of microorganisms such as putrefying bacteria is inhibited, and the number of enterococcus, pseudomonas and hydrogen sulfide producing bacteria in each gram of fish meat in the fermentation process is obviously reduced. Potassium chloride is added to replace sodium chloride, accumulation of volatile basic nitrogen and biogenic amine in the fermentation process is inhibited, the volatile basic nitrogen is as low as 28.87 mg / 100g, putrescine is 137.45 mg / Kg, and tyramine is 18.34 mg / Kg.

The invention discloses a phenylpyruvate decarboxylase mutant and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The purpose of the present invention is to increase the yield of tyrosol. The phenylpyruvate decarboxylase mutant provided by the invention is obtained by mutating any one of the 56th site, the 92th site, the 93th site, the 106th site, the 115th site, the 153th site, the 178th site, the 188th site, the 214th site, the 220th site, the 234th site, the 242th site, the 273th site, the 285th site, the 371th site, the 416th site, the 581th site and the 621th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The phenylpyruvate decarboxylase is mainly used for high-efficiency phenylpyruvate decarboxylase and application thereof in de novo synthesis of tyrosol.

The invention belongs to the technical field of sugarcane germ detection, and particularly relates to a dual-mode detection method for sugarcane smut fungi. According to the dual-mode detection method for the sugarcane smut fungi, a fluorescence-naked eye reading dual-mode lateral chromatography platform for detecting the sugarcane smut fungi genes is developed by utilizing a composite material RuPd (at) vCDs and based on a rolling circle amplification and Cas12a active RCA-Cas platform. The method depends on a target chain as a primer, triggers an RCA process to generate a DNA product, and then activates the trans-cleavage activity of Cas12a, so that DNAC is catalytically cleaved. CDs is used as a luminescent probe for detecting a fluorescence signal, and RuPd bimetallic can catalytically decompose hydrogen peroxide by using own catalase-like activity, so that an alginate-tyramine conjugate forms gel for measuring a flow distance. The dual-mode detection realizes ultra-sensitive and rapid detection of the smut smut of sugarcane, and shows a wide application prospect.

The present application relates to the field of agricultural cultivation technology, and more particularly to a method for improving the low-temperature stress tolerance of cherry tomato seedlings by exogenous tyramine. The method selects cherry tomato seedlings at the 4-leaf 1-heart stage, and sprays the cherry tomato seedlings in the experimental group with a tyramine water solution at a concentration of 1-2.5 mg / L at 5 pm every day. The spraying is performed according to the standard of covering the leaves with mist droplets and no dripping. The cherry tomato seedlings in the normal temperature and low temperature control groups are sprayed with distilled water. The influence of tyramine on cherry tomatoes under low-temperature stress is observed. The test results prove that exogenous tyramine significantly alleviates the growth of cherry tomatoes under low-temperature stress, reduces the MDA and H2O2 contents in the leaves of cherry tomatoes, increases the proline and soluble sugar contents, and enhances the activity of antioxidant enzymes, thereby effectively reducing the damage of low-temperature stress to cherry tomato plants.

This invention discloses a strain of *Lactobacillus sarcodactylis* with stable biogenic amine degradation ability and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of *Lactobacillus sarcodactylis* L18 from fermented soybean paste, capable of degrading biogenic amines. This strain can simultaneously degrade eight common biogenic amines: tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine. The *Lactobacillus sarcodactylis* L18 screened in this invention exhibits rapid growth, strong environmental adaptability, and stable amine-reducing performance. It can continuously exert its biogenic amine degradation effect under low to medium-high salt, acidic to weakly alkaline conditions, and common fermentation temperature conditions, making it particularly suitable for the effective control of biogenic amines such as tyramine in fermented foods.

The invention relates to the field of biological agents, particularly discloses an immune color developing reagent for detecting malignant tumors in urine and application of the immune color developing reagent, and is used for solving the problems that in bladder cancer urine exfoliated cell detection, an acridine orange single staining color developing image is poor in bladder tumor specific epitope recognition, heavy in background interference and high in spectrum overlapping and manual interpretation subjectivity. The color developing reagent comprises a reagent A, a reagent B, a reagent C and a reagent D. The reagent A is provided with an antibody complex and acridine orange aiming at tumor cell target antigens of bladder parts in urine shedding, and the reagent B comprises a fluorescently-labeled secondary antibody composition and an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent A, and the reagent D is provided with an enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the enzyme-labeled secondary antibody-tyramine deposition amplification composition corresponding to the reagent D. In the application, AI imaging analysis is adopted, and layered identification labels and acridine orange dyeing negative and positive labels are calibrated; through multi-target immune color development, two sets of color separation amplification schemes and AI imaging analysis, the bladder tumor specific epitope recognition capability, the image quality and the image analysis accuracy of urine cast-off cell detection are improved.

The invention discloses a psychrobacter capable of efficiently degrading biogenic amine and application of the psychrobacter. The strain is a strain FJNU-SIO36, the classification name of the strain is Psychrobacter sp., the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is January 6, 2025, the preservation number is CGMCC No.33316, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The strain FJNU-SIO36 does not generate biogenic amines, can degrade nine biogenic amines such as putrescine, cadaverine, histamine and tyramine at the same time, and especially has a degradation rate of 100% for putrescine and cadaverine, which is of great significance to degradation of biogenic amines in fermented food. The strain FJNU-SIO36 provided by the invention can be used for degrading biogenic amines, and is wide in application range, low in cost and simple to operate.

The invention discloses bio-based benzoxazine resin as well as a preparation method and application thereof, and belongs to the technical field of resin materials, salicylaldehyde and tyramine are taken as raw materials and subjected to condensation reaction in an ethanol solvent to obtain Schiff base, double bonds in the Schiff base are reduced by sodium borohydride, a secondary amine structure is formed, and a phenolic compound intermediate is obtained. Taking the phenolic compound intermediate as a matrix, introducing alkynyl or cyano into the molecular structure of the matrix to obtain a bio-based benzoxazine monomer, and heating and curing to obtain the bio-based benzoxazine resin. Due to the action of the flexible chain segment ethylidene, the bio-based benzoxazine monomer prepared by the invention has excellent processability. The bio-based benzoxazine resin prepared by the preparation method disclosed by the invention has good heat resistance, thermal stability, flame retardance and dielectric property due to the synergistic effect between the flexible chain segment ethylidene and the rigid group, the ring opening polymerization of the bifunctional benzoxazine monomer and extra crosslinking provided by alkynyl or cyano group, and the bio-based benzoxazine resin prepared by the preparation method disclosed by the invention has good heat resistance, thermal stability, flame retardance and dielectric property.

The invention relates to the technical field of biological medicine, and provides a pharmaceutical composition containing alzvudine and a VEGFR-TKI inhibitor and application of the pharmaceutical composition in resisting liver cancer. In-vivo and in-vitro experiments prove that the alzvudine and the VEGFR-TKI have an obvious synergistic effect in liver cancer treatment, and the pharmaceutical composition provided by the invention can still ensure and improve the anti-tumor curative effect on the basis of obviously reducing the clinical use dosage of the core targeted drug VEGFR-TKI, and has a good application prospect. The pharmaceutical composition provided by the invention can effectively reduce the incidence rate of core targeted drug-related adverse reactions, has good safety, has significant advantages in the aspects of improving the treatment compliance and life quality of patients, and provides a new strategy for solving the clinical problem that the curative effect and toxicity in targeted treatment of liver cancer are difficult to consider at the same time.

The invention discloses an amido bond synthetase mutant with improved stability and application of the amido bond synthetase mutant. The amino acid sequences of the amido bond synthetase mutant are SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11. Compared with wild type zymoprotein, the half-life period of the mutant constructed by the invention can be prolonged to 1.54 times of the original half-life period under the condition of the optimal reaction temperature. The mutant constructed by the invention uses ferulic acid as a substrate to catalytically produce feruloyl tyramine, the highest conversion rate can reach 100%, and the production cost can be greatly reduced.

This invention discloses a visual detection method and application of biogenic amines based on a carbon dot / quantum dot ratio fluorescent probe, belonging to the field of food safety detection technology. Addressing the problems of existing biogenic amine detection methods, such as complex operation, difficulty in rapid on-site screening, and susceptibility to interference from single fluorescent signals, this invention prepares a ratio fluorescent probe based on graphene carbon dots (G-CDs) and near-infrared quantum dots (QD700). This probe requires no chemical modification and achieves a synergistic response of G-CDs fluorescence enhancement and QD700 fluorescence quenching through the Spe-induced aggregation effect, achieving a detection limit of 0.0097 μM and a response time faster than 2 seconds. Combined with the DEIM intelligent model, it can accurately distinguish spermine, putrescine, cadaverine, histamine, and tyramine. Integrated into a paper-based smartphone platform, it enables visual identification of shrimp freshness (red for fresh, green for spoiled), showing a high correlation with the TVB-N standard method (R²=0.95), suitable for rapid on-site monitoring.