142 results about "Tyramine" patented technology

The invention relates to a nanotechnology-based trace protein detection method, which combines enzyme-linked immunosorbent assay technology, tyramine signal amplification technology and the aggregation phenomenon of gold nanoparticles modified by different biological molecules, so an experimental method used for detecting trace proteins such as prostate specific antigen (PSA) and the like is established. The method comprises the following steps of: fixing an antibody aiming at the protein to be detected (such as the PSA) on the surface of a substrate; incubating another antibody with horseradish peroxidase (HRP) activity of the protein to be detected (such as the PSA) after capturing the protein to be detected in a sample, wherein the HRP catalyzes biotin-tyramide to generate biotin deposition under certain conditions; further amplifying a signal by using the aggregation phenomenon of the gold nanoparticles modified by biotin-labeled DNA and the gold nanoparticles modified by streptavidin; performing silver staining; and performing data analysis on an experimental result by using software. The method has the advantages of extremely low detection limit, wider detection range, capacity of detecting the antigen in a rabbit serum with complex compositions, and important application prospect.

The invention discloses a silk fibroin-gelatin double crosslinked hydrogel and a preparation method thereof. The method comprises the following steps: (1) preparing tyramine grafting modified gelatin;(2) preparing a silk fibroin solution; (3) preparing thermoreversible silk fibroin/tyramine modified gelatin hydrogel; and (4) preparing silk fibroin-gelatin double crosslinked hydrogel. The method of the invention has simple process, and the prepared double crosslinked silk fibroin-gelatin composite hydrogel has stronger mechanical properties than the gelatin or silk fibroin hydrogel alone, hasthe advantages of gelatin or silk fibroin hydrogel, and makes up for the defects of gelatin or silk fibroin hydrogel. The hydrogel with excellent biocompatibility and mechanical properties has a broadapplication prospect in the field of tissue engineering vascularization.

The invention relates to novel molecule detection signal amplification technique, which is characterized in that the molecule detection signal amplification technique is a biomolecule detection method with high sensitivity which is formed by streptavidin, an antigen, an antibody, polypeptide and the like which are marked by quantum dots through tyramine signal amplification and silver strengthening dyeing signal amplification. The detection method comprises the following steps: the biomolecule to be detected is combined with nucleic acid, the antibody, the antigen, the polypeptide and the like which are fixed on a solid phase material, the quantum dots are introduced to molecule to be detected through biomolecule specificity combination, silver strengthening dyeing is carried out on the quantum dots by using silver dyeing reagents, and signals are amplified. Obtained signals can be scanned through common optical scanners, analyzed through common imaging instruments or observed through naked eyes. The detection method achieves high sensitivity detection of the biomolecule on one hand, and reduces application cost of biomolecule detection on the other hand.

The invention discloses an adenosine detecting method based on a micro-fluidic chip and a nucleic adapter technology. Biotin-modified adenosine nucleotide adapter and biotin-modified casein are fixed on the surface of an avidin-modified microsphere to form a functional microsphere through a biotin-avidin combining method; capture probes modified with horseradish peroxidase and sulfydryl are modified on nanometer particles as signal marks; functional nanometer particles flow to a micro-fluidic microsphere array to detect a detection area of a chip and hybrid with a microsphere array; after elution, an adenosine solution flows to be incubated with hybridized microspheres; a peroxidase substrate solution flows in; biotinylated tyramine is combined with the casein on the surface of the microsphere; the avidin-marked quantum dot flows in after elution; qualification and quantification are carried out on the adenosine by calculating a ratio of a fluorescence amount of the microsphere surface after adenosine inflows and a fluorescence amount of the microsphere surface before the adenosine inflows. The adenosine detecting method provided by the invention can be used for detecting and analyzing a target at high sensitivity, so that the specific and accurate quantitative analysis of the adenosine in a serum sample is realized.

The invention discloses a chitosan hydrogel. The structural unit of the chitosan hydrogel is shown in the specification; and in the structural unit, m is a natural number in a range of 400-700, and n is a natural number in a range of 30-100. The invention also discloses a preparation method of the chitosan hydrogel, and an application of the chitosan hydrogel. The method comprises the following steps: polyethylene glycol and tyramine molecules are grafted to a chitosan molecular chain in order to obtain a highly-water-soluble chitosan derivative, and the derivative is processed under the action of a crosslinking reagent to obtain the chitosan hydrogel. The process of the preparation method is simple, and the prepared chitosan hydrogel has the advantages of good physical, chemical and biological performances, good histocompatibility, environmental protection, and non-toxicity, and can be safely and effectively applied in restoration of wound tissues.

The invention relates to composition comprising a dextran-tyramine conjugate and a conjugate selected from the group consisting of chondroitin sulphate-tyramine, collagen-tyramine, chitosan-tyramine, chitosan-phloretic acid, gelatine-tyramine, heparan sulphate-tyramine, keratin sulphate-tyramine, hyaluronic acid-tyramine and heparin-tyramine.

The invention discloses a lactobacillus plantarum and its application in the high-acid yellow wine production for acid modulation, belonging to the field of rice wine brewing technology. The lactobacillus plantarum CGMCC No.12757, isolated from rice wine fermented mash. The lactobacillus plantarum comprises of total acid of rice wine produced of more than 17.5g/L (in lactic acid), lactic acid content of 70% or more accounting for the total organic acid, 14% (v / v) or more of alcohol content, 52mg / L biogenic amine content (the total amount of putrescine, histamine and tyramine), far lower than the rancid rice wine in the storage process, which can produce high-acid rice wine for acid modulation, greatly facilitating the production of low-grade rice wine.

The invention discloses a formula of multifunctional polymethylmethacrylate. The formula comprises polymethyl methacrylate and additives, wherein the additives include a fire retardant, an antistatic agent, a dispersing agent, a colorant and a coupling agent; the fire retardant is triphenyl phosphate; the antistatic agent is ethyoxyl lauro tyramine; the dispersing agent is triethylhexyl phosphoric acid; the colorant is palm oil acid diester; and the coupling agent is ammonia propyltriethoxysilane. Prepared polymethylmethacrylate has the characteristics of flame retardance, static electricity resistance, good internal dispersity, diversified colors, low production cost and the like.

Ethamine compound is obtained by condensing starting materials of 6-bromoisovanillin and tyramine under catalysis of NaBH4 in carbinol. In formylation of ethamine compound, aminic acid and formaldehyde of same amount are used to produce formyl compound, with temperature between 90 DEG C. and 110 DEG C., 8h. Racemic bromonarwedine is obtain by oxidation and cyclization of the formyl compound. Racemic narwedine is obtained by reduction reaction under catalysis of NaCO2H, PPh3, Pd(OAc)2. Racemic narwedine is transformed into (-)-narwedine by a process of crystal inoculation. (-)-narwedine is reduced into unsaturated ketone by (-)-N-methyl ephedrine as chiral reagent, whereby optically active alcohol is obtained, and (-)-galanthamine is also obtained.

The invention discloses enzymatic catalysis crosslinking reduction-responsive hyaluronic acid microgel and a preparation method thereof. The hyaluronic acid microgel is prepared by taking sulfhydrylation hyaluronic acid as a raw material, taking horseradish peroxidase as a catalyst, taking tyramine hydrochloride as an enzymatic catalysis reaction substrate and combining the method that horseradish peroxidase is catalytically crosslinked with sulfydryl to generate a disulfide bond through an inverse emulsion method. The disulfide bond crosslinking structure in the microgel can be broken in the presence of reductive substances such as dithiothreitol, reductive glutathione hormone and L-cysteine, and then the good reduction responsiveness is given to the microgel. The hyaluronic acid microgel can achieve controlled release on the reduction responsiveness of loaded doxorubicin hydrochloride and can be applied to controlled release of tumor-targeted drugs. According to the preparation method, a crosslinking agent does not need to be additionally added into an inverse emulsion system to cure the microgel, the toxicity influence of the crosslinking agent is avoided, the biocompatibility of the microgel is guaranteed, the reaction conditions are mild, and the technological processes are simple.

The invention discloses a total alkaloids extractive of piper hancei and application thereof, belongs to the technical field of medicine, and in particular relates to a total alkaloids extractive of traditional Chinese medicine piper hancei and application thereof. The extractive is obtained from the traditional Chinese medicine piper hancei, and comprises the following alkaloid components: sinoacutine amide, hairy grama piperine, long pepper amide A, pepper a-amanitine, Guinea pepper, piperine, piperettine, piperovatine, N-p-tonka-bean acyl tyramine, N-trans- asafetida acyl tyramine, birthwort lactam AIIIa, birthwort lactam II, and 2E, 4E-N- isobutyl-7-3,4-methinedioxy phenyl-2, 4-diene heptamide, long pepper nerinine and a derivate thereof, wherein the total content of the total alkaloids components is counted by 5 to 100 percent by weight.

The invention discloses enzyme for degrading biogenic amine in soy sauce, and belongs to the technical field of fermentation. A sequence of multi-copper oxidase crude enzyme provided by the inventionis as shown in SEQ ID NO.1. The enzyme disclosed by the invention has different degrees of degradation functions on biogenic amine of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine and spermidine in the soy sauce, wherein the concentration of the tyramine of which the content in the soy sauce is the most is 540.71 mg/L. After adding the multi-copper oxidase crude enzymeprovided by the invention, the content of the biogenic amines is reduced by 100 mg/L within 24 hours; compared with an existing technology for reducing the content of the biogenic amine by 58.57 mg/Lby adding pure enzyme in a biogenic amine mixed solution for 24 hours, the degradation time is short, and the effect is good.

Combinations comprising vanadium (IV)/(V) compounds and pharmaceutically acceptable amines selected from the group of semicarbazide-sensitive amine oxidase (SSAO) substrates are insulin mimickers. Preferred vanadium (IV)/(V) compounds are vanadyl salts, vanadyl complexes and vanadates (e.g. sodium orthovanadate). Preferred amines are tyramine and benzylamine. A here-discovered synergism between the vanadium compound and the amine makes the effective concentration of vanadate in the combination one order of magnitude lower than the corresponding of vanadate alone. Consequently the combination has much lower toxicity than the known vanadium compound alone, which is a crucial advantage of the former for its use in the treatment and/or prevention of Diabetes mellitus.

The invention relates to a detection method with a gene chip, in particular to a visualized detection method which can improve the sensitivity of gold label silver stain of a gene chip to a great extent. The detection method mainly comprises the following steps that: gene marker biotin molecule of a sample to be tested is used to hybridize with the gene chip. At first, TSA treatment is performed on the hybridized chip; vast Tyramine is precipitated around a reaction site under the catalysis of enzymes; gold nanoparticles with streptavidin are added and nanoscale gold particles are connected to target molecules; silver stain reagent is used to color the hybridized gene chips to observe and analyze signals. A TSA procedure is added in the method provided by the invention based on a single gold label silver stain detection, the Biotin-Tyramine reagent used for TAS is simple to prepare, avoids purification and is easy to preserve and stable in performance. Compared with the single gold label silver stain detection method, the detecting sensitivity of the gene chips can be improved by ten to one hundred times.

The invention discloses a method for artificially synthesizing galanthamine, which uses isovanillin and bromine as the raw materials. After the raw materials are subjected to substitution reaction, the reaction and industrial chemicla tyramine are subjected to amination and formylation to obtain formamide, the formamide is subjected to reaction to obtain a derivative of racemic narwedine, the derivative of racemic narwedine is reduced into racemic narwedine, N-methylephedrine is used to reduce unsaturated ketone and tartaric acid is used to carry out resolution to obtain levogyrate galanthamine. The method not only can conveniently and rapidly prepare the galanthamine, but also adopts artificial preparation so as to have little limination in the preparation process.

The invention relates to an enterococcus faecalis with the collection number of CGMCC No.3164. A bacterial colony is cultured on an MRS agar culture medium at the temperature of 37 DEG C for 24 hours to be gathered; the bacterial colony is white, thallus is ovate, extends along chain direction, is in pair or in short chain, is Gram-negative, generates lactic acid when fermenting glucose and is elliptic under a common microscope. When being applied, the bacterial colony is inoculated into a test tube filled with culture medium A to stand at the temperature of 37 DEG C to be cultured for 24 hours; after being repeatedly activated five times, the bacteria solution is inoculated into a culture medium B to stand at the temperature of 37 DEG C to be cultured for four days; the cultivated bacteria solution is decentralized at 1000 rpm for 10 minutes; and supernate is extracted to obtain high concentration tyramine solution. The culture medium A is an MRS liquid culture medium of 0.1% of tyrosine, and the culture medium B is an MRS liquid culture medium of 0.005% of pyridoxal phosphate and 0.1% of tyrosine.

The invention discloses a non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on a peptide sensor. According to a sensing interface building method, carbon nanohorns, cysteamine functional golden cone and polyglycine are used as substrates; corn zeranol antibodies (Ab) are further immobilized; tyramine functionalized nanometer rutile type TiO2 mesoscopic crystals are used for immobilizing peptide chains with the specific sequences as a photoelectric probe; the peptide chain has the effect of simulating the zearalenone, the zearalenone can compete with the markedphotoelectric probe to combine the corn zeranol antibodies immobilized on the sensing interface. The photoelectric probe combined onto the sensing interface can further combine with tyramine-Rutile TiO2 mesoscopic crystal compounds under the catalysis effect of horseradish peroxidase; optoelectronic signals can be further amplified. On the basis, the non-toxic photoelectrochemistry competition immunoassay method on the corn zeranol can be built.

The invention provides a method for rapidly detecting multiple biogenic amines in a dairy product by virtue of combination of high performance thin layer chromatography and adjustable surface enhanced raman spectroscopy, and belongs to the technical field of food detection. The method comprises the steps of preparation of nano silver colloid, preparation of samples, separation based on high performance thin layer chromatography, HPTLC-SERS detection and the like. The HPTLC-SERS detection method for identifying structures of the multiple biogenic amines (tyramine, putrescine and cadaverine) is established, and has the advantages of economical efficiency, rapidness, simplicity and convenience; furthermore, by virtue of the selectivity enhancement function of a new synergist, the application range of HPTLC-SERS detection is expanded, and a new visual field is opened for directly and rapidly detecting a chromatoplate for chemical derivatization.

The invention discloses a rapid analysis method of biogenic amine in fish. The common biogenic amine (nine types of biogenic amine comprising agmatine, histamine, tyramine, spermine, spermidine, 2-phenylethylamine, tryptamine, cadaverine and putrescine) is analyzed in a targeted manner by taking 5-sulfosalicylic acid as an extraction solvent, adopting bead agitating and grinding as an extraction method, taking benzoyl chloride as a derivatization reagent and adopting an LC (liquid chromatogram)-triple tandem quadrupole mass spectrometry. The biogenic amine is detected in a multi-reactions monitoring manner by taking ion pairs with optimum response, which are obtained by optimization, as quantitative ion pairs and taking ion pairs with following response as auxiliary qualitative ion pairs.According to the method, the sample extraction time and the biogenic amine derivation time can be greatly shortened, so that the analysis time of the whole analysis process is greatly shortened. The analysis method has the advantages of being simple in sample pretreatment, rapid in derivation, high in detection sensitivity, high in repeatability and the like.