4 results about "Heparan sulphate" patented technology

This invention belongs to the field of heparin sodium extraction technology, specifically involving a method for graded extraction of chondroitin sulfate B, heparan sulfate and heparin sodium. It consists of the following steps: (1) dissolving crude heparin in deionized water, centrifuging to remove insoluble matter from the heparin solution, and taking the supernatant to obtain crude heparin solution; (2) adjusting the pH of the crude heparin solution from step (1) to 8-9, then adding A98 resin, and adsorbing at 60-62℃; (3) using NaCl solution to elute the resin adsorbed in step (2) twice, then adding anhydrous ethanol three times for precipitation, collecting the precipitate, adjusting the pH to 9-10, adding potassium permanganate solution at 75-80℃ for oxidization for 30-40 min, filtering with diatomaceous earth, taking the filtrate, adding anhydrous ethanol to the filtrate for precipitation, and drying to obtain heparan sulfate; this invention has a high extraction rate and good extraction effect, and can also extract heparan sulfate and heparin sodium during the extraction of chondroitin sulfate B.

The application belongs to the technical field of bioengineering technology, and particularly relates to a heparanase and a preparation method and application thereof. The amino acid sequence of the heparanase (HPSE) is shown as SEQ ID NO. 2. The heparanase (WJS HPSE) provided in the application can specifically degrade HS glycan substrates, which are white jade snail polysaccharide (WJS PS) containing IdoA2S-GlcNAc disaccharide repeating units, heparin or HS. In addition, the heparanase (WJS HPSE) provided in the application has high thermal stability, has optimal enzyme activity at 57-67 DEG C and pH=4.0, significantly improves the stability and reuse efficiency in a catalytic process, and thus improves the application value of the HPSE.

The invention belongs to the technical field of biomedical materials, and discloses a bone-imitating organic matrix composite fiber material for bone defect repair as well as a preparation method and application of the bone-imitating organic matrix composite fiber material. The bone-imitating organic matrix composite fiber material is a composite fiber membrane composed of core-shell structure composite fiber filaments prepared through coaxial electrostatic spinning. The core of the core-shell structure composite cellosilk is obtained by compounding polycaprolactone, mesoporous bioactive glass and multi-component glycosaminoglycan, and the shell of the core-shell structure composite cellosilk is obtained by compounding polycaprolactone, silk fibroin and nerve growth factors; the multi-component glycosaminoglycan is prepared from the following components in parts by mass: 6 to 27 parts of hyaluronic acid, 28 to 39 parts of chondroitin sulfate, 4 to 24 parts of dermatan sulfate, 1 to 29 parts of heparin, 14 to 42 parts of heparan sulfate and 0.16 to 0.41 part of keratin sulfate. The bone-imitating organic matrix composite fiber material has a good application prospect in preparation of medicines or medical instruments for promoting bone defect repair.