9 results about "Heparan sulphate" patented technology

The application provides a fermented vegetable oil and a preparation method thereof. The preparation method comprises the following steps: crushing a vegetable oil raw material and mixing the same with water to obtain a raw material solution; adding a biological enzyme to the raw material solution to perform enzymolysis, and after enzyme inactivation, an enzymolysis product is obtained; then, a fermentation bacteria is added to perform fermentation, sterilization and centrifugation to obtain an oily liquid; the oily liquid, 1,2-dilauroylphosphatidyl ethanolamine, a self-assembled structure regulator and propylene glycol are uniformly mixed, and then added into water to obtain a liposome suspension of the fermented vegetable oil; 3-thiophene malonic acid and / or flower acid are first added into the liposome suspension of the fermented vegetable oil, and then heparan sulfate and calcium chloride are added, and after reaction, the fermented vegetable oil encapsulated in a carrier is obtained. The preparation method of the fermented vegetable oil has good long-term stability, and can also improve the poor compatibility of the fermented vegetable oil with a water-based system.

The present invention relates to a method of screening for osteoarthritis (OA) and rheumatoid arthritis (RA) in a subject suffering from arthritis or suspected of suffering from arthritis and a method of discriminating between OA and rheumatoid arthritis RA comprising determining the level of one or more glycosaminoglycan (GAG) properties according to the invention in a sample of bodily fluid. The invention also provides methods for monitoring OA or RA progression in a subject suffering from arthritis.

This invention belongs to the field of heparin sodium extraction technology, specifically involving a method for graded extraction of chondroitin sulfate B, heparan sulfate and heparin sodium. It consists of the following steps: (1) dissolving crude heparin in deionized water, centrifuging to remove insoluble matter from the heparin solution, and taking the supernatant to obtain crude heparin solution; (2) adjusting the pH of the crude heparin solution from step (1) to 8-9, then adding A98 resin, and adsorbing at 60-62℃; (3) using NaCl solution to elute the resin adsorbed in step (2) twice, then adding anhydrous ethanol three times for precipitation, collecting the precipitate, adjusting the pH to 9-10, adding potassium permanganate solution at 75-80℃ for oxidization for 30-40 min, filtering with diatomaceous earth, taking the filtrate, adding anhydrous ethanol to the filtrate for precipitation, and drying to obtain heparan sulfate; this invention has a high extraction rate and good extraction effect, and can also extract heparan sulfate and heparin sodium during the extraction of chondroitin sulfate B.

The application belongs to the technical field of bioengineering technology, and particularly relates to a heparanase and a preparation method and application thereof. The amino acid sequence of the heparanase (HPSE) is shown as SEQ ID NO. 2. The heparanase (WJS HPSE) provided in the application can specifically degrade HS glycan substrates, which are white jade snail polysaccharide (WJS PS) containing IdoA2S-GlcNAc disaccharide repeating units, heparin or HS. In addition, the heparanase (WJS HPSE) provided in the application has high thermal stability, has optimal enzyme activity at 57-67 DEG C and pH=4.0, significantly improves the stability and reuse efficiency in a catalytic process, and thus improves the application value of the HPSE.

The invention provides fermented vegetable oil and a preparation method thereof.The preparation method of the fermented vegetable oil comprises the following steps that vegetable oil raw materials are smashed and mixed with water, and a raw material solution is obtained; adding biological enzyme into the raw material liquid, performing enzymolysis and enzyme deactivation to obtain an enzymolysis product, then adding zymophyte, and performing fermentation, sterilization and centrifugation to obtain oily liquid; uniformly mixing the oily liquid, 1, 2-dilauroyl phosphatidyl ethanolamine, a self-assembly structure regulator and propylene glycol, adding the mixture into water, and uniformly mixing to obtain a lipidosome suspension of the fermented vegetable oil; 3-thiophenemalonic acid and / or chamalonic acid are / is added into the lipidosome suspension of the fermented vegetable oil, heparan sulfate and calcium chloride are added into the lipidosome suspension, and the fermented vegetable oil encapsulated in the carrier is obtained after reaction. According to the preparation method of the fermented vegetable oil, the obtained fermented vegetable oil is good in long-term stability, and the problem that the fermented vegetable oil is poor in compatibility with a water-based system can be solved.

The invention belongs to the technical field of biomedical materials, and discloses a bone-imitating organic matrix composite fiber material for bone defect repair as well as a preparation method and application of the bone-imitating organic matrix composite fiber material. The bone-imitating organic matrix composite fiber material is a composite fiber membrane composed of core-shell structure composite fiber filaments prepared through coaxial electrostatic spinning. The core of the core-shell structure composite cellosilk is obtained by compounding polycaprolactone, mesoporous bioactive glass and multi-component glycosaminoglycan, and the shell of the core-shell structure composite cellosilk is obtained by compounding polycaprolactone, silk fibroin and nerve growth factors; the multi-component glycosaminoglycan is prepared from the following components in parts by mass: 6 to 27 parts of hyaluronic acid, 28 to 39 parts of chondroitin sulfate, 4 to 24 parts of dermatan sulfate, 1 to 29 parts of heparin, 14 to 42 parts of heparan sulfate and 0.16 to 0.41 part of keratin sulfate. The bone-imitating organic matrix composite fiber material has a good application prospect in preparation of medicines or medical instruments for promoting bone defect repair.

The application relates to the field of filter materials, in particular to a melt-blown PBT non-woven fabric composite filter material and a preparation method and application thereof. The melt-blown PBT non-woven fabric composite filter material comprises a care net layer, a filter paper layer and a bottom layer PBT melt-blown fabric, and the raw material of the bottom layer PBT melt-blown fabric comprises heparinized TPU and PBT in a weight ratio of 1: (3-5). The preparation method of the heparinized TPU is as follows: 1) TPU is dissolved to obtain a TPU solution, and the temperature is lowered to 38-42 DEG C; 2) heparin sulfate is dissolved to obtain a heparin sulfate solution; 3) the heparin sulfate solution is added dropwise into the TPU solution while stirring, and shear homogenization is carried out; and 4) drying is carried out to obtain heparinized TPU. The filter material is prepared by compounding TPU modified by heparin sulfate and PBT, the thrombosis risk in the blood filtration process is significantly reduced, and the platelet reduction rate in the blood filtration is as low as 25.4-14.9%.

Methods for treating hepatic ischemia-reperfusion (I / R) injury in a subject are disclosed. In some embodiments, the methods include providing a subject suffering from or at risk of suffering from hepatic I / R injury; and administering to the subject one or more heparan sulfate (HS) compounds. In some embodiments, the one or more HS compounds comprise about 5 to about 18 sugar units, optionally about 12 to about 18 sugar units. In some embodiments, the one or more HS compounds comprise about 12 sugar units.