162 results about "Disaccharide" patented technology

The invention belongs to the technical field of bioengineering technologies, and particularly relates to heparanase sulfate as well as a preparation method and application thereof. The amino acid sequence of the heparanase sulfate (HPSE) is as shown in SEQ ID NO. 2. The HS carbohydrate chain substrate capable of being specifically degraded by the heparan sulfate enzyme (WJS HPSE) provided by the invention is white jade snail polysaccharide (WJS PS) containing an IdoA2S-GlcNAc disaccharide repeating unit, heparin or HS. In addition, the heparan sulfate enzyme (WJS HPSE) provided by the invention is high in thermal stability and has optimal enzyme activity at the temperature of 57-67 DEG C and the pH value of 4.0, and the stability and reutilization efficiency of the heparan sulfate enzyme in a catalytic process are remarkably improved, so that the application value of the HPSE is improved.

The invention relates to a particulate composition comprising, or substantially consisting of, urea, an inhibitor, preferably a urease inhibitor and / or a nitrification inhibitor, and an additive, wherein the additive comprises components (i), (ii), and optionally (iii): (i) one or more polyhydric alcohols; preferably one or more carbohydrates, preferably selected from monosaccharides, disaccharides, and oligosaccharides; (ii) a first polymer, preferably an amine-containing polymer; more preferably a polyvinylamine or a polyalkyleneimine; and (ii) optionally, a second polymer, preferably a polar polymer; more preferably a water-soluble polar polymer. The invention further relates to a process for producing the particulate composition, an additive for a particulate composition, and the use of the particulate composition as a fertilizer.

The invention discloses a freeze-drying protective agent composition, a freeze-drying protective agent, application of the freeze-drying protective agent, a probiotic freeze-dried product and a preparation method of the probiotic freeze-dried product. The freeze-drying protective agent composition comprises the following components in parts by weight: 0.5-4 parts of tremella polysaccharide; the relative molecular weight of the tremella polysaccharide is 1 KDa to 10 KDa; 0.5 to 4 parts of disaccharide; and 0.5-2 parts of sugar alcohol. The freeze-drying protective agent composition and water are uniformly mixed and then sterilized to obtain the freeze-drying protective agent, and the freeze-drying protective agent is used for preparing probiotic freeze-dried products and can effectively ensure the activity and stability of probiotics.

The invention discloses a sugarcane polyphenol and mulberry leaf extract compound raw material and application thereof.The sugarcane polyphenol and mulberry leaf extract compound raw material comprises sugarcane polyphenol and mulberry leaf extract, the polyphenol content of the sugarcane polyphenol is larger than or equal to 20%, the DNJ content of the mulberry leaf extract is larger than or equal to 10%, and the concentration of the polyphenol and the DNJ is 0.1-2% after the sugarcane polyphenol and the mulberry leaf extract are compounded; by compounding the sugarcane polyphenol and the mulberry leaf extract, the active and passive transfer effects of glucose in small intestines are effectively blocked, so that the rate of the glucose entering blood is controlled, the blood glucose reaction after carbohydrate food is eaten is reduced, the decomposition and absorption of the intestinal tract to carbohydrates such as starch and disaccharide are delayed, the postprandial blood sugar is stabilized, and the blood sugar content is reduced. By reasonably adjusting the concentration of polyphenols and DNJ in the compound extract to be 0.1-2%, the combination of blocking and dredging can be realized, a synergistic effect is generated, more comprehensive and more stable management of blood sugar is realized, and meanwhile, natural crops are adopted, so that the product quality safety is ensured.

The invention provides traditional Chinese medicine fermented disaccharide encapsulated nano anti-dandruff, hair-growing and oil-controlling shampoo and a preparation method, and relates to the field of daily chemical products, and the shampoo comprises the following components: a compound fermentation product, lactobacillus crispatus, lactobacillus plantarum, a disaccharide activity protection system, a basic component and a nano oil-controlling component; the compound leavening is prepared from cacumen biotae, radix polygoni multiflori, radix ginseng, fructus cnidii and orchid; the disaccharide activity protection system comprises maltotetraose and trehalose; the disaccharide activity protection system wraps a fermented material formed by fermenting the compound fermentation product, the lactobacillus crispatus and the lactobacillus plantarum to form a disaccharide glassy state layer; the nano oil control component is prepared from calamine powder and chitosan-sodium alginate; the basic component is prepared from tea bran, radix sophorae flavescentis, herba ecliptae, radix notoginseng, fructus ligustri lucidi, tea saponin microcapsules and tremella polysaccharide. According to the invention, through fermentation conversion of the orchid, disaccharide glassy state protection and slow release of the nano calamine, activity enhancement, stable retention and accurate release are realized, and the effects of removing dandruff, relieving itching, controlling oil and growing hair are achieved.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles by disaccharide and compound enzyme. According to the method, an enzymolysis-disaccharide solution extraction coupling method is created for the first time to extract the plant extracellular vesicles, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 40-300 nm. Compared with PBS (Phosphate Buffer Solution) extraction treatment, the total protein extraction amounts of lactose, cane sugar and maltose solutions are respectively increased by 20.04% + / -4.50%, 35.00% + / -5.74% and 36.45% + / -2.68%. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The invention belongs to the field of biomolecule detection, and discloses a polysaccharide high-throughput detection method and application. The method can be used for detecting the heparin precursor, a sample does not need to be purified, the heparin precursor in a complex sample can be directly recognized and degraded by enzyme specificity to generate disaccharide, and then the content of the disaccharide of the heparin precursor is detected through high-throughput EchoMS, so that the heparin precursor is subjected to rapid, high-throughput, qualitative and quantitative analysis; the method is used for screening and evaluating elements influencing heparin precursor synthesis, screening and evaluating heparin precursor synthesis strains, optimizing the heparin precursor fermentation process and the like. The method can also be used for high-throughput detection of glycosaminoglycans such as hyaluronic acid, heparin and chondroitin sulfate and other polysaccharides such as pullulan, curdlan, xanthan gum and glucan, and can be used for structural analysis of polysaccharides.

A dry stable probiotic composition is provided. The composition comprises one or more viable probiotic microorganisms, one or more hydrolyzed proteins, one or more disaccharides, one or more oligosaccharides, and one or more polysaccharides, but not trehalose. The composition has viability of at least 1×1010 CFU / g, and a viability loss of less than 1 log unit / g after one month at a temperature of 40° C. and a relative humidity of 33%. Also provided are methods for preparing the dry stable probiotic composition.

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

Intumescent composition comprising an acid donor (A), a carbon donor (B), a binder polymer (C) and a blowing agent (D). The acid donor (A) is selected from the group consisting of ammonium polyphosphate, ammonium dihydrogen phosphate, ethylenediamine phosphate, ammonium pentaborate, guanylurea phosphate, diguanidine phosphate and mixtures thereof. The carbon donor (B) is selected from the group consisting of glucose, arabinose and other monosaccharides, lactose, maltose and other disaccharides, starch, cellulose, dextrin and other polysaccharides, sorbitol, erythritol, pentaerythritol, dipentaerythritol, mannitol and other polyhydric alcohols and mixtures thereof. The blowing agent is provided in the form of microsphere particles comprising a thermoplastic polymer shell encapsulating a propellant, which microsphere particles are expandable microspheres.

The present invention relates to a metal nitrate hydrate catalyst and a method for preparing 5-hydroxymethylfurfural, using same and, more specifically, may provide: a catalyst which comprises a metal nitrate hydrate and is to prepare 5-hydroxymethylfurfural (HMF) from at least one selected from the group consisting of monosaccharides and disaccharides; and a method for preparing 5-hydroxymethylfurfural (HMF), the method comprising (a) a step of preparing 5-HMF by reaction in the presence of the catalyst from a sugar comprising at least one selected from the group consisting of monosaccharides and disaccharides. The present invention provides high 5-HMF yield and selectivity, biological and process stability, and economic efficiency by using inexpensive monosaccharides and / or disaccharides.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

Provided herein are methods for preparing a product including predominantly prebiotic oligosaccharides by non-enzymatic methods of glycosylation of monosaccharides, disaccharides, and / or polysaccharides. The methods may include mixing one or more types of monosaccharides, disaccharides, and / or polysaccharides with an effective amount of a dehydrating acid at a temperature sufficient to form a product including predominantly prebiotic oligosaccharides.

A main object of the present invention is to provide a novel technique for activating T cell immune responses.SOLUTION: Solution to Problem The present inventors have found that the above problems can be solved by a compound represented by the general formula (I): wherein Sugar represents any monosaccharide, disaccharide, or trisaccharide; and R represents a hydrogen atom or an acyl group having 2 to 5 carbon atoms, or a salt thereof.SELECTED DRAWING: None

The invention discloses a disaccharide monosubstituted derivative of Lamellarin D and N. The structure of the disaccharide monosubstituted derivative is as follows: R1 and R2 are respectively and independently selected from one of H, methyl, benzyl, acetyl and disaccharide, R3 is selected from H, benzyl or disaccharide, R4 is selected from H, methylsulfonyl or disaccharide, and at least one of R1-R4 is disaccharide; the disaccharide group is one of cellobiose group, acetylated cellobiose group, lactosyl group, acetylated lactosyl group, maltose group and acetylated maltose group. According to the present invention, at least one disaccharide group is introduced into R1-R4 to obtain the disaccharide mono-substituted derivative of Lamellarin D and N, the proliferation inhibition activity of tumor cells is improved with the compound and the monosaccharide substituted derivative, and the inhibition effect on non-solid tumor cell line Jurkat is significantly improved by about 5 times;

To provide a tablet that is easy to swallow and also has reduced production cost, and a method of producing the same.SOLUTION: A tablet has a naked tablet containing a gelator, and at least one selected from sugar alcohol, monosaccharide, disaccharide, oligosaccharide, cellulose, cellulose derivative, starch, starch derivative and starch decomposition product. Preferably, the gelator is at least one selected from xanthan gum, locust bean gum, guar gum, mannan, glucomannan, hyaluronan, agar, alginic acid, tamarind gum, psyllium seed gum, tara gum, carrageenan, acacia gum, gum arabic, ghatti gum, tragacanth gum, karaya gum, cassia gum, rhamsan gum, welan gum, macrophomopsis gum, curdlan, pullulan, gellan gum, pectin, and soybean polysaccharide, gelatin, collagen, polyglutamic acid, polylactic acid, polyglutamic acid and the like.SELECTED DRAWING: None

To provide a crack prevention agent capable of effectively preventing cracking or chipping of dried food materials during a manufacturing process and transportation, the dried food materials having volume and weight significantly reduced as a result of removal of moisture, and being brittle and easily cracked.SOLUTION: In a method for producing dried food materials using a dry food material crack prevention agent, the dried food material crack prevention agent comprises, as an active ingredient, a hydrogenated saccharide composition containing a total of 40 wt.% or more of hydrogenated monosaccharides and hydrogenated disaccharides.SELECTED DRAWING: None

A stable dry composition is provided. This composition comprises one or more viable microorganisms, at least 50% by weight of one or more hydrolyzed proteins, and less than 10% by weight of one or more sugars selected from the group consisting of monosaccharides, disaccharides, and combinations thereof, each weight percentage based on the total weight of the dry composition. Preferably, the composition contains no monosaccharides or disaccharides. The composition may further comprise one or more oligosaccharides, one or more polysaccharides, one or more carboxylic acid salts, or a combination thereof. The composition may have a viability of at least 1 x 10¹⁰ CFU / g and a loss of viability of less than 1 log unit / g after 84 days at 40°C and 33% relative humidity. Methods for preparing the stable dry composition are also provided.

A Bifidobacterium longum subsp longum produced by a method of growing the Bifidobacterium longum subsp longum in a culture medium, the culture medium containing a fructose disaccharide, FOS or combination thereof. A method for treatment or prevention of inflammatory bowel disease, celiac disease, non-celiac gluten sensitivity, gluten ataxia, dermatitis herpetiformis or wheat allergy in an individual, the method including administering a combination of (i) Bifidobacterium longum subsp longum and (ii) a fructose disaccharide, FOS or a combination thereof to the individual.

Use of a fructose disaccharide or fructooligosaccharide, for increasing protein production in Bifidobacterium longum subsp longum.

The objective of this invention is to provide a means for controlling the proliferation of *Streptobacter* spp. A composition for controlling the proliferation of *Streptobacter* spp. is provided, comprising any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar. In one embodiment, the composition comprises galactosylkohlbiose as any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar.

According to the invention, there is provided a solid pharmaceutical composition formulation for nasal delivery of an opioid antagonist, comprising a pharmacologically-effective amount of an opioid antagonist and a pharmaceutically-acceptable carrier. The compositions are preferably in the form of a powder produced by spray-drying, which are subsequently loaded into single use nasal applicators. Preferred pharmaceutically-acceptable carriers in this regard include disaccharides (e.g. lactose or trehalose) and dextrins (e.g. cyclodextrins or maltodextrins), preferably spray-dried together in combination. Compositions may further comprise an alkyl saccharide, preferably a sucrose ester, such as sucrose monolaurate. The compositions and applicators may be employed in the treatment of opioid overdose in subjects.

The present disclosure provides stable inoculant compositions and methods for enhancing the survival and / or stability of microorganisms in an inoculant composition. In some embodiments, the microorganisms in an inoculant compositions are stabilized by the presence of one or more sugar alcohols, humic acids and / or fulvic acids and one or more maltodextrins, monosaccharides, disaccharides, oxidation control components and / or UV protectants.

The application belongs to the field of biochemistry, and particularly relates to a preparation method of a sodium hyaluronate oligosaccharide composition, and further discloses the prepared composition and application. The preparation method of the sodium hyaluronate oligosaccharide comprises the following steps: taking macromolecular sodium hyaluronate as raw material, preparing an acidic aqueous solution, adding ethanol to perform a precipitation reaction, separating supernatant, removing impurities through an ultrafiltration membrane, performing a second ethanol precipitation reaction, separating precipitate, purifying the precipitate through a reverse-phase ODS column, and obtaining the sodium hyaluronate oligosaccharide with required performance. The preparation method has the advantages of simple and easy-to-operate preparation process, suitable reaction conditions, simple and smooth production process, greatly reduced production period and cost, and a product yield of more than 96%. The sodium hyaluronate oligosaccharide composition prepared by the method comprises disaccharide, tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide and dodecasaccharide, has better product stability, excellent water solubility, good transdermal permeability, good moisturizing property, good oral absorption and other advantages.

This invention provides a novel technology that allows for a more pronounced citrus fruit flavor in citrus fruit juice-flavored alcoholic beverages containing distilled spirits, where the total concentration of monosaccharides and disaccharides (excluding sugar alcohols) is less than 0.5 g / 100 mL based on the volume of the beverage. [Solution] A citrus fruit juice flavored alcoholic beverage containing distilled spirits as a raw material, wherein the total concentration of monosaccharides and disaccharides excluding sugar alcohols is less than 0.5 g / 100 mL based on the volume of the beverage, and the citrus fruit juice flavored alcoholic beverage contains linalool, nonanal, and neryl acetate.

The present invention concerns sugar beet pulp which is capable of absorbing and / or holding an amount of water that is at least 14 times the dry weight of the sugar beet pulp.The present invention further concerns a method for improving the water absorption capacity and / or water holding capacity of sugar beet pulp, comprising the steps of:(a) providing sugar beet material;(b) optionally extracting mono- and disaccharides from the sugar beet material provided in step (a) at a temperature below 75° C. to provide spent sugar beet pulp;(c) subjecting the sugar beet material provided in step (a) or the spent sugar beet pulp provided in step (b) to a heating step at a temperature of at least 85° C. to obtain sugar beet pulp with improved water absorption capacity and / or water holding capacity;(d) optionally removing mono- and disaccharides from the sugar beet pulp obtained in step (c).

The present invention relates to a method for producing disaccharides using beta-glucosidase and its cofactors, and a composition for inducing enzyme production in a Trichoderma strain, which contains the produced disaccharides. Specifically, the method comprises inducing beta-glucosidase and a divalent metal ion, manganese ion (Mn ion), as a cofactor, on a glucose substrate. 2+ ), magnesium ion (Mg 2+ ), zinc ion (Zn 2+ ) or copper ions (Cu 2+ ), the disaccharide production rate can be increased and disaccharides can be obtained at a high concentration. In addition, since the composition with a high content of disaccharides produced by the production method of the present invention has an excellent effect of inducing enzyme production in Trichoderma strains, it is possible to improve the enzyme productivity of Trichoderma by applying a fermentation process that utilizes the produced disaccharides at a high concentration.

This invention provides a means for controlling butyrate production in the intestine. The objective of this invention is to provide a means for controlling the proliferation of butyrate-producing bacteria, represented by the genus *Faecalibacterium*. This invention provides a composition for controlling the proliferation of butyrate-producing bacteria, preferably *Faecalibacterium*, comprising any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar. In one embodiment, the composition comprises galactosylkohlbiose as any one selected from kohlbiose and oligosaccharides with kohlbiose as a constituent sugar.