The application belongs to the field of
biochemistry, and particularly relates to a preparation method of a
sodium hyaluronate
oligosaccharide composition, and further discloses the prepared composition and application. The preparation method of the
sodium hyaluronate
oligosaccharide comprises the following steps: taking macromolecular
sodium hyaluronate as
raw material, preparing an acidic
aqueous solution, adding
ethanol to perform a
precipitation reaction, separating supernatant, removing impurities through an
ultrafiltration membrane, performing a second
ethanol precipitation reaction, separating precipitate, purifying the precipitate through a reverse-phase ODS column, and obtaining the
sodium hyaluronate oligosaccharide with required performance. The preparation method has the advantages of simple and easy-to-operate preparation process, suitable
reaction conditions, simple and smooth production process, greatly reduced production period and cost, and a product yield of more than 96%. The
sodium hyaluronate oligosaccharide composition prepared by the method comprises
disaccharide,
tetrasaccharide, hexasaccharide, octasaccharide, decasaccharide and dodecasaccharide, has better product stability, excellent water
solubility, good transdermal permeability, good moisturizing property, good oral absorption and other advantages.