162 results about "Vasculogenesis" patented technology

The invention belongs to the technical field of artificial prosthesis materials, and particularly relates to a medical implant with a lithium disilicate coating and a preparation method thereof, and the preparation method comprises the following steps: (1) placing a prefabricated matrix in a high-temperature environment for staged sintering, and then carrying out surface polishing, cleaning and drying treatment; (2) mixing lithium ethoxide and tetraethoxysilane at room temperature, and reacting for 2-4 hours while stirring to obtain lithium disilicate sol; the preparation method comprises the following steps: preparing a prefabricated substrate, soaking the prefabricated substrate in lithium disilicate sol for 3-7 minutes, performing sol permeation for 3-10 minutes under the negative pressure of more than 30 mm Hg, adding an ethanol water solution to initiate gelation reaction, taking out the prefabricated substrate after the reaction is completed, drying and sintering to obtain the medical implant with the lithium disilicate coating. According to the method, the uniform and continuous lithium disilicate coating which is not easy to fall off can be formed on the surface of the prefabricated matrix of the implant, so that the surface performance of the implant is improved, and the osteogenesis promoting performance and the angiogenesis promoting performance of the implant are optimized.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The invention discloses a chiral supramolecular fiber structure design based on glutamic acid derivative and pyrazine co-assembly, a preparation method and application in bone regeneration and vascular regeneration. The chiral supramolecular assembly disclosed by the invention is formed by co-assembling a glutamic acid derivative and pyrazine, is an all-organic three-dimensional spiral fiber material with an accurate and controllable three-dimensional topological configuration, and can realize coupling regeneration of bones and blood vessels by cooperatively regulating and controlling dual action mechanisms of osteogenic differentiation and angiogenesis; the compound can be used for efficiently treating complex bone defects and related bone diseases. In addition, raw materials and reagents are simple and easy to obtain, reaction conditions are mild, the preparation method is simple, and good application prospects are achieved.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.

The invention discloses a titanium-magnesium hollow screw system capable of promoting fracture healing and a manufacturing method. The titanium-magnesium hollow screw system comprises a titanium-based supporting structure and a magnesium filling layer. The titanium-based supporting structure comprises a main body part, the surface of the main body part is provided with an imitated bone trabecula part, and a plurality of bone gaps are formed in the imitated bone trabecula part; wherein bone gaps are filled with the magnesium filling layer, and the magnesium filling layer is combined with the titanium-based supporting structure in a physical embedding mode; the titanium-based supporting structure bears and maintains initial fixation and long-term bearing, so that the strength and the stability of the screw are ensured; the surface imitated bone trabecula and the communicated bone gaps increase the surface area and provide a physical channel for vascularization and bone tissue ingrowth, so that synostosis is promoted; the magnesium filling layer releases magnesium ions after being locally controllably degraded, so that osteogenesis and angiogenesis are favorably stimulated, the local biological environment is improved, instant mechanical support after an operation is ensured, bone healing is promoted through local biological activity release and structural orientation, the healing time is favorably shortened, and the healing quality is favorably improved.

The invention discloses a black phosphorus hydrogel microneedle patch loaded with trained mesenchymal stem cells as well as a preparation method and application of the black phosphorus hydrogel microneedle patch, and belongs to the technical field of biological medicines. The micro-needle patch comprises a hydrogel substrate layer loaded with black phosphorus nanosheets and a hydrogel needle tip part for packaging the stem cells. The mesenchymal stem cells are subjected to combined domestication with low oxygen and high active oxygen, so that the anti-oxidative stress, anti-inflammatory and angiogenesis promoting capabilities of the mesenchymal stem cells are remarkably enhanced. After the patch is subcutaneously delivered, the black phosphorus component can efficiently remove bacteria and reduce drug resistance; after being domesticated, the stem cells can adapt to the immune microenvironment of the wound surface, and the immune regulation, inflammation inhibition and tissue regeneration promotion are synergistically realized by continuously releasing anti-inflammatory and angiogenesis promoting factors, so that the refractory wound surface caused by immune disorders such as systemic lupus erythematosus (SLE) and the like is effectively treated. The invention provides an innovative treatment strategy for chronic inflammatory wound healing.

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.

The invention discloses a preparation method of a micron-sized cell-matrix co-polymer biological material. The preparation method comprises the following steps: firstly, inducing SHED cells in an ultra-low adsorption plate by using an alpha-MEM culture medium containing serum, GlutaMAXI, vitamin C and the like to form micron-sized spheres (SHED spheres); secondly, taking human dentin, and carrying out mechanical crushing, EDTA (Ethylene Diamine Tetraacetic Acid) demineralization, grinding and sterilization to prepare 1-100 microns of dentin matrix particles (DDMPs); and finally, by taking the GelMA hydrogel as a carrier, entrapping SHED spphere and DDMPs, and carrying out 405nm photo-crosslinking for 30 seconds to form the hydrogel. The co-polymer can significantly promote angiogenesis and osteogenesis coupling regeneration in a bone defect model, and is suitable for oral cavity and cranio-maxillofacial bone repair.

A composition is provided comprising a cell population and pharmaceuticals derived from human gingival fibroblasts, present in proportions not found in natural gingival tissue, but having a cellular phenotype preferentially selected to express proteins favorable for angiogenesis and anti-inflammatory effects, while reducing the formation of cell populations that promote tumorigenesis and / or metalloproteinases that inhibit tissue regeneration. A method for producing such a composition that increases proliferation several times over compared to previously known methods is provided, and a method for using such a composition in a wide range of human cell therapies is also provided.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

The invention provides a broad-spectrum combined medicine for treating colorectal cancer. The broad-spectrum combined medicine is prepared from the following components in parts by weight: 100 parts of purified water, 38 to 90 parts of tripterygium wilfordii alkaloid, 38 to 90 parts of tripterygium hypoglaucum alkaloid, 28 to 85 parts of chamaejasmine, 28 to 85 parts of gelsmium elegans alkaloid and 28 to 85 parts of xanthoxylin. The medicine is prepared from the following raw materials in parts by weight: 16 to 80 parts of ganoderan, 16 to 80 parts of pinellia ternate sitosterol, 16 to 80 parts of radix rehmanniae praeparata, 16 to 80 parts of astragaloside, 16 to 80 parts of oldenlandia diffusa alkaloid, 16 to 80 parts of sculellaria barbata alkaloid, 16 to 80 parts of tanshinone, 16 to 80 parts of carthamin, 16 to 80 parts of honeysuckle chlorogenic acid and 16 to 80 parts of pericarpium citri reticulatae flavone. By integrating anti-tumor components with multiple action mechanisms, tripterygium wilfordii alkaloid, tripterygium hypoglaucum alkaloid and the like, tumor cell proliferation can be directly inhibited, and apoptosis can be induced; tanshinone, emodin and the like can inhibit tumor angiogenesis; ganoderma lucidum polysaccharides, astragaloside and the like can activate the immune system of the body, enhance the activity of T cells and NK cells, and form double strikes of direct killing and immune surveillance.

The invention provides a sequential slow-release hydrogen sulfide nerve repair conduit and a preparation method thereof, and belongs to the technical field of biological materials. The sequential slow-release hydrogen sulfide nerve repair conduit comprises degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel and a pH response hydrogen sulfide donor, wherein the degradable polymer conduit is filled with the degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, and the pH response hydrogen sulfide donor is loaded on the hydrogel. In the application, the pH response donor can respond to an acidic microenvironment of degradation of polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, release hydrogen sulfide and relieve the acidic environment generated by degradation of the hydrogel at the same time, and form a dual supply mechanism with cysteine (endogenous hydrogen sulfide precursor) obtained by degradation of the hydrogel; nerve regeneration and angiogenesis are synergistically promoted, and oxidative stress is regulated; and the hydrogel is accelerated to degrade during excessive oxidative stress, so that hydrogen sulfide is released as required, and the core problems of insufficient blood supply, unbalanced acidic microenvironment and slow nerve regeneration are synchronously solved.

The invention discloses bioactive porous high-density polyethylene foam and a preparation method thereof, and belongs to the technical field of medical implant materials. The preparation method comprises the following steps: (1) mixing high-density polyethylene powder and template particles, carrying out hot press molding, ultrasonically removing the template particles in a solvent, and finally washing and drying to obtain porous high-density polyethylene foam; and (2) sequentially soaking and modifying the porous high-density polyethylene foam in a hydrophilic improver, a biological active agent and an antibacterial agent, and drying to obtain the porous high-density polyethylene foam. The preparation method disclosed by the invention is simple, and the obtained bioactive high-density polyethylene foam with the porous structure has good biological activity, hydrophilicity and excellent mechanical properties, is beneficial to cell adhesion, material exchange, fibroblast ingrowth and angiogenesis, can be used as a craniomaxillofacial non-bearing area bone defect repair material, and has wide application prospects. And the method has a huge practical application prospect.

Provided is the use of RAP2C in the breeding of Blcattle black cattle, belonging to the technical field of molecular biological breeding. RAP2C can be used for the breeding of Blcattle black cattle, and individuals having a high RAP2C content are screened as high-quality calves and alternative breeding cattle. In the present disclosure, it is proved by means of experiments that RAP2C can promote the differentiation of vascular endothelial progenitor cells to regulate placental angiogenesis, enhance placental blood flow and increase placental nutrient transport efficiency in Blcattle black cattle, thereby regulating calf birth weight. The present disclosure provides a new direction for the breeding of high-quality beef cattle of Blcattle black cattle.

The subject invention pertains to a novel method for treating Peripheral Arterial Disease (PAD) by leveraging the angiogenic potential oftype 2 cytokines IL-4 and IL-13 to enhance the efficacy of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) and induced endothelial cells derived from fibroblasts (iECs). This present invention aims at enhancing the muscle regeneration and revascularization for obese and diabetes individuals.

Various methods for isolating cell populations from adipocyte tissue and / or blood and quantifying levels of vascular endothelial growth factor receptors (VEGFR) on the isolated cell populations are provided. Also provided are methods of evaluating or modifying angiogenic therapy in a subject.

The invention belongs to the technical field of biomedicine and nano crossing, and particularly relates to a biomineralization-based nucleic acid aptamer functionalized DNA nano-structure compound as well as a preparation method and application thereof. The compound has a tetrahedral structure and comprises a functional DNA tetrahedral framework and a calcium phosphate coating coated on the surface of the functional DNA tetrahedral framework, the functionalized DNA tetrahedral framework is formed by self-assembly of four DNA single chains; wherein the 5'end of one DNA single chain is modified with a nucleic acid aptamer. The bionic calcium coating is used as a protective shell, so that the in-vivo stability and the angiogenesis promoting activity of the Apt02 are remarkably improved. Meanwhile, by virtue of the specific spatial configuration of the tetrahedral DNA nanostructure, the compound can release calcium ions after being taken by cells, so that osteogenic differentiation is further promoted, and a new strategy for synergistic promotion is provided for vascularized bone regeneration.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

The application discloses a wound dressing and a preparation method and application thereof; the wound dressing comprises polymer flexible fibers and cobalt-iron layered double hydroxide nanosheets dispersed in the polymer flexible fibers; the cobalt-iron layered double hydroxide nanosheets contain a cobalt vacancy structure in a crystal lattice. The wound dressing has a strong "electron capture effect" due to the cobalt vacancy. The wound dressing can physically capture electrons on a respiratory transmission chain of a pathogenic bacterial cell membrane, interferes with transmembrane proton transfer and ATP synthesis, directly kills the bacteria through "metabolic starvation", and further has a broad-spectrum physical bactericidal function independent of antibiotics. The wound dressing has excellent superoxide dismutase-like and catalase-like activities, can long-acting capture and remove active oxygen such as superoxide anions and hydrogen peroxide in an inflammatory microenvironment of a wound, promotes macrophages to polarize to a M2 phenotype for promoting repair, and has anti-inflammatory and pro-angiogenic functions.

The present invention provides a composition of matter comprising a population of cells and an agent, which is derived from human gingival fibroblasts and has a cell phenotype present in a proportion that is not found in the natural gingival tissue, the cell phenotype being preferentially selected to express a protein beneficial to angiogenesis and anti-inflammatory action, meanwhile, cell populations of metalloproteinase formation that promotes tumorigenicity and / or inhibits tissue regeneration are reduced. The present invention provides methods of producing the compositions that can achieve multiple proliferation improvements compared to previously known methods, and methods of using the compositions in a wide range of human cell therapies.

The invention discloses hydrogel based on umbilical cord tissue and chorion tissue, which is prepared by a method comprising the following steps: (1) taking a placenta, shearing the umbilical cord and separating the chorion; (2) respectively carrying out tissue crushing on the umbilical cord and the chorion, and centrifugally collecting precipitates; mixing, washing, centrifuging and collecting precipitates; (3) freezing with liquid nitrogen, and then melting; repeatedly freezing and thawing; adding a buffer solution containing triton-X-100, and carrying out soaking treatment; rinsing, centrifuging and collecting precipitates; (4) adding urea, oscillating, centrifuging and collecting precipitate; freezing and drying to obtain freeze-dried powder; (5) dissolving the freeze-dried powder in hydrochloric acid, adding pepsin, and reacting; and adjusting the pH value to 7-7.5 to obtain the hydrogel. The hydrogel is applied to culture of cells, tissues or organoids, and is applied to preparation of a culture medium with the effect of promoting angiogenesis. The method is of great significance to development in the fields of regenerative medicine, disease modeling, medicine development and the like.

The invention relates to the technical field of medical treatment, in particular to a preparation method of injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel for superficial tumor treatment, which comprises the following steps: pretreating raw materials, preparing BiOCl nanosheets by a solvothermal method, washing and drying for later use; according to the manufacturing method of the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel for superficial tumor treatment, the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel is embedded into injectable, conductive and thermosensitive hydrogel composed of PluronicF127 (F127) and reduced graphene oxide (rGO), the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel is used for combined treatment of breast cancer, remarkable ROS outbreak and effective breast cancer cell ablation are induced through in-vitro and synergistic therapy, toxicity to normal cells is minimum, and the injectable BiOCl (at) CuOF127 (at) rGO thermosensitive hydrogel can be applied to in-vivo and in-vivo treatment of breast cancer. In-situ breast tumors treated with US + NIR show obvious fading, proliferation and angiogenesis reduction, apoptosis and immunogen cell death pathway activation and good biocompatibility, an accurate and minimally invasive strategy is established, piezoelectric catalysis and photo-thermal conversion are combined, and the in-situ breast tumors are used for effective breast cancer treatment.

The present disclosure provides for one or more embodiments for treating retinal degenerative disorders in a subject by administering to the subject an effective amount of allogeneic mesenchymal stem cells to the subject's suprachoroidal area. Allogeneic mesenchymal stem cells, when used in the disclosed treatment regimen, may exhibit anti-inflammatory, immunomodulatory, anti-fibrotic, pro-vascular, and / or pro-regenerative processes. Thus, allogeneic mesenchymal stem cells may be understood to have the potential to target and treat multiple pathological targets implicated in retinal degenerative disorders, including retinal disorders characterized by drusen accumulation, some of which have inherited forms.

The present invention provides decidual placental mesenchymal stem cells and a method for culturing the same, which comprises introducing an induction step into a conventional method for culturing mesenchymal stem cells to highly express decoy receptor 3 (DcR3) in the decidual placental mesenchymal stem cells.Furthermore, the present invention provides a use of the decidual placental mesenchymal stem cells in the manufacture of a drug for promoting angiogenesis.

The invention discloses a method for preparing a vascular organ chip from human umbilical artery stem cells, and belongs to the field of biomedical engineering and tissue engineering. The method comprises the following steps: separating and culturing human umbilical artery stem cells to the third generation; preparing a PDMS micro-fluidic chip containing a liquid inlet channel, a liquid outlet channel, a growth channel and a central culture chamber; a blood vessel precursor aggregate is obtained through cell digestion, aggregate construction, mesoderm induction and blood vessel pedigree induction, the blood vessel precursor aggregate is inoculated into a Coll-1-Matrigel gel modified chip through a two-step method, and dynamic perfusion culture is conducted in combination with a culture medium containing VEGF-A and FGF-2. And finally, the structure and the function of the vascular organ are verified through immunofluorescent staining and fluorescent microbead perfusion. The problems that in the prior art, cell sources are limited, culture conditions are single and the like are solved, the high angiogenesis potential of the human umbilical artery stem cells and a dynamic culture system are utilized to construct a high-bionic-degree vascular network, and the method can be applied to drug screening, vascular pathological model construction and regenerative medicine research.

The invention discloses a space-time responsive multifunctional 3D printing bone repair scaffold as well as a preparation method and application thereof. PDGF-BB is loaded on a quick-release carrier, MgP is loaded on a slow-release carrier, and the multifunctional 3D printing bone repair scaffold with MgP loaded on the inner layer and PDGF-BB loaded on the outer layer is prepared through 3D printing. Wherein the PDGF-BB is used as an immunomodulator, the MgP is used as a regeneration inducer, and after the carrier is implanted into a body, according to different carrier degradation rates, the PDGF-BB is degraded and released in a space-time responsive manner to realize early immunoregulation and endothelial cell recruitment, and then magnesium ions and phosphate ions are degraded and released to synergistically promote vascularization and osteogenesis in middle and later periods with the PDGF-BB. In-vitro and in-vivo experiments prove that the 3D printing bone repair scaffold provided by the invention has immunoregulation, angiogenesis promotion and osteogenesis properties, can be used for repairing inflammatory bone injury, and is particularly suitable for repairing inflammatory bone injury caused by diabetes.

This invention, entitled "A Method and Product for Pretreatment of Mesenchymal Stem Cells and Its Application in Stroke Drugs," belongs to the field of biotechnology. The technical problem to be solved is to improve the function and therapeutic effect of mesenchymal stem cells. The key technical solution is a method for pretreatment of mesenchymal stem cells, including: culturing cells using an activation medium, culturing cells using a directional medium, and culturing cells under hypoxic conditions using an adaptation medium; the activation medium contains complete medium, IFN-γ, TNF-α, and IL-1β; the directional medium contains complete medium, sodium valproate, 5-azacytidine, and all-trans retinoic acid; the adaptation medium is a complete medium containing basal medium and free of animal serum. The pretreatment method of this invention enhances the anti-inflammatory, neuroprotective, and angiogenic capabilities of mesenchymal stem cells, showing significant therapeutic effects on stroke, and since it does not use animal serum, it meets clinical requirements.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.