96 results about "Vasculogenesis" patented technology

The invention belongs to the technical field of artificial prosthesis materials, and particularly relates to a medical implant with a lithium disilicate coating and a preparation method thereof, and the preparation method comprises the following steps: (1) placing a prefabricated matrix in a high-temperature environment for staged sintering, and then carrying out surface polishing, cleaning and drying treatment; (2) mixing lithium ethoxide and tetraethoxysilane at room temperature, and reacting for 2-4 hours while stirring to obtain lithium disilicate sol; the preparation method comprises the following steps: preparing a prefabricated substrate, soaking the prefabricated substrate in lithium disilicate sol for 3-7 minutes, performing sol permeation for 3-10 minutes under the negative pressure of more than 30 mm Hg, adding an ethanol water solution to initiate gelation reaction, taking out the prefabricated substrate after the reaction is completed, drying and sintering to obtain the medical implant with the lithium disilicate coating. According to the method, the uniform and continuous lithium disilicate coating which is not easy to fall off can be formed on the surface of the prefabricated matrix of the implant, so that the surface performance of the implant is improved, and the osteogenesis promoting performance and the angiogenesis promoting performance of the implant are optimized.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The invention discloses a titanium-magnesium hollow screw system capable of promoting fracture healing and a manufacturing method. The titanium-magnesium hollow screw system comprises a titanium-based supporting structure and a magnesium filling layer. The titanium-based supporting structure comprises a main body part, the surface of the main body part is provided with an imitated bone trabecula part, and a plurality of bone gaps are formed in the imitated bone trabecula part; wherein bone gaps are filled with the magnesium filling layer, and the magnesium filling layer is combined with the titanium-based supporting structure in a physical embedding mode; the titanium-based supporting structure bears and maintains initial fixation and long-term bearing, so that the strength and the stability of the screw are ensured; the surface imitated bone trabecula and the communicated bone gaps increase the surface area and provide a physical channel for vascularization and bone tissue ingrowth, so that synostosis is promoted; the magnesium filling layer releases magnesium ions after being locally controllably degraded, so that osteogenesis and angiogenesis are favorably stimulated, the local biological environment is improved, instant mechanical support after an operation is ensured, bone healing is promoted through local biological activity release and structural orientation, the healing time is favorably shortened, and the healing quality is favorably improved.

The invention discloses application of a tripterygium wilfordii exosome in preparation of an anti-cervical cancer medicine and a tumor angiogenesis inhibiting medicine, and relates to the field of biological medicine. The medicinal plant exosome derived from tripterygium wilfordii is successfully separated, and it is proved that the medicinal plant exosome can regulate related pathways through delivery of functional nucleic acid molecules to inhibit proliferation and migration of cervical cancer cells and promote active oxygen related apoptosis so as to be used for tumor treatment. The tripterygium wilfordii exosome can further act on vascular endothelial cells in a tumor microenvironment, the migration function of Hela cells is inhibited, and then the effect of resisting cell proliferation in the tumor microenvironment is achieved. The tripterygium wilfordii exosome has the advantages of being simple in preparation method, remarkable in tumor growth resistance and tumor cell migration inhibition effect, good in biocompatibility, high in delivery efficiency and the like, and can be used as a novel nano-drug for tumor treatment.

The invention discloses a preparation method of a micron-sized cell-matrix co-polymer biological material. The preparation method comprises the following steps: firstly, inducing SHED cells in an ultra-low adsorption plate by using an alpha-MEM culture medium containing serum, GlutaMAXI, vitamin C and the like to form micron-sized spheres (SHED spheres); secondly, taking human dentin, and carrying out mechanical crushing, EDTA (Ethylene Diamine Tetraacetic Acid) demineralization, grinding and sterilization to prepare 1-100 microns of dentin matrix particles (DDMPs); and finally, by taking the GelMA hydrogel as a carrier, entrapping SHED spphere and DDMPs, and carrying out 405nm photo-crosslinking for 30 seconds to form the hydrogel. The co-polymer can significantly promote angiogenesis and osteogenesis coupling regeneration in a bone defect model, and is suitable for oral cavity and cranio-maxillofacial bone repair.

A composition is provided comprising a cell population and pharmaceuticals derived from human gingival fibroblasts, present in proportions not found in natural gingival tissue, but having a cellular phenotype preferentially selected to express proteins favorable for angiogenesis and anti-inflammatory effects, while reducing the formation of cell populations that promote tumorigenesis and / or metalloproteinases that inhibit tissue regeneration. A method for producing such a composition that increases proliferation several times over compared to previously known methods is provided, and a method for using such a composition in a wide range of human cell therapies is also provided.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

The invention provides a sequential slow-release hydrogen sulfide nerve repair conduit and a preparation method thereof, and belongs to the technical field of biological materials. The sequential slow-release hydrogen sulfide nerve repair conduit comprises degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel and a pH response hydrogen sulfide donor, wherein the degradable polymer conduit is filled with the degradable temperature-sensitive polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, and the pH response hydrogen sulfide donor is loaded on the hydrogel. In the application, the pH response donor can respond to an acidic microenvironment of degradation of polyethylene glycol monomethyl ether-poly (cysteine-glycolic acid) hydrogel, release hydrogen sulfide and relieve the acidic environment generated by degradation of the hydrogel at the same time, and form a dual supply mechanism with cysteine (endogenous hydrogen sulfide precursor) obtained by degradation of the hydrogel; nerve regeneration and angiogenesis are synergistically promoted, and oxidative stress is regulated; and the hydrogel is accelerated to degrade during excessive oxidative stress, so that hydrogen sulfide is released as required, and the core problems of insufficient blood supply, unbalanced acidic microenvironment and slow nerve regeneration are synchronously solved.

Various methods for isolating cell populations from adipocyte tissue and / or blood and quantifying levels of vascular endothelial growth factor receptors (VEGFR) on the isolated cell populations are provided. Also provided are methods of evaluating or modifying angiogenic therapy in a subject.

The invention belongs to the technical field of biomedicine and nano crossing, and particularly relates to a biomineralization-based nucleic acid aptamer functionalized DNA nano-structure compound as well as a preparation method and application thereof. The compound has a tetrahedral structure and comprises a functional DNA tetrahedral framework and a calcium phosphate coating coated on the surface of the functional DNA tetrahedral framework, the functionalized DNA tetrahedral framework is formed by self-assembly of four DNA single chains; wherein the 5'end of one DNA single chain is modified with a nucleic acid aptamer. The bionic calcium coating is used as a protective shell, so that the in-vivo stability and the angiogenesis promoting activity of the Apt02 are remarkably improved. Meanwhile, by virtue of the specific spatial configuration of the tetrahedral DNA nanostructure, the compound can release calcium ions after being taken by cells, so that osteogenic differentiation is further promoted, and a new strategy for synergistic promotion is provided for vascularized bone regeneration.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The application discloses a wound dressing and a preparation method and application thereof; the wound dressing comprises polymer flexible fibers and cobalt-iron layered double hydroxide nanosheets dispersed in the polymer flexible fibers; the cobalt-iron layered double hydroxide nanosheets contain a cobalt vacancy structure in a crystal lattice. The wound dressing has a strong "electron capture effect" due to the cobalt vacancy. The wound dressing can physically capture electrons on a respiratory transmission chain of a pathogenic bacterial cell membrane, interferes with transmembrane proton transfer and ATP synthesis, directly kills the bacteria through "metabolic starvation", and further has a broad-spectrum physical bactericidal function independent of antibiotics. The wound dressing has excellent superoxide dismutase-like and catalase-like activities, can long-acting capture and remove active oxygen such as superoxide anions and hydrogen peroxide in an inflammatory microenvironment of a wound, promotes macrophages to polarize to a M2 phenotype for promoting repair, and has anti-inflammatory and pro-angiogenic functions.

The invention discloses hydrogel based on umbilical cord tissue and chorion tissue, which is prepared by a method comprising the following steps: (1) taking a placenta, shearing the umbilical cord and separating the chorion; (2) respectively carrying out tissue crushing on the umbilical cord and the chorion, and centrifugally collecting precipitates; mixing, washing, centrifuging and collecting precipitates; (3) freezing with liquid nitrogen, and then melting; repeatedly freezing and thawing; adding a buffer solution containing triton-X-100, and carrying out soaking treatment; rinsing, centrifuging and collecting precipitates; (4) adding urea, oscillating, centrifuging and collecting precipitate; freezing and drying to obtain freeze-dried powder; (5) dissolving the freeze-dried powder in hydrochloric acid, adding pepsin, and reacting; and adjusting the pH value to 7-7.5 to obtain the hydrogel. The hydrogel is applied to culture of cells, tissues or organoids, and is applied to preparation of a culture medium with the effect of promoting angiogenesis. The method is of great significance to development in the fields of regenerative medicine, disease modeling, medicine development and the like.

The present disclosure provides for one or more embodiments for treating retinal degenerative disorders in a subject by administering to the subject an effective amount of allogeneic mesenchymal stem cells to the subject's suprachoroidal area. Allogeneic mesenchymal stem cells, when used in the disclosed treatment regimen, may exhibit anti-inflammatory, immunomodulatory, anti-fibrotic, pro-vascular, and / or pro-regenerative processes. Thus, allogeneic mesenchymal stem cells may be understood to have the potential to target and treat multiple pathological targets implicated in retinal degenerative disorders, including retinal disorders characterized by drusen accumulation, some of which have inherited forms.

The present invention provides decidual placental mesenchymal stem cells and a method for culturing the same, which comprises introducing an induction step into a conventional method for culturing mesenchymal stem cells to highly express decoy receptor 3 (DcR3) in the decidual placental mesenchymal stem cells.Furthermore, the present invention provides a use of the decidual placental mesenchymal stem cells in the manufacture of a drug for promoting angiogenesis.

The invention discloses a method for preparing a vascular organ chip from human umbilical artery stem cells, and belongs to the field of biomedical engineering and tissue engineering. The method comprises the following steps: separating and culturing human umbilical artery stem cells to the third generation; preparing a PDMS micro-fluidic chip containing a liquid inlet channel, a liquid outlet channel, a growth channel and a central culture chamber; a blood vessel precursor aggregate is obtained through cell digestion, aggregate construction, mesoderm induction and blood vessel pedigree induction, the blood vessel precursor aggregate is inoculated into a Coll-1-Matrigel gel modified chip through a two-step method, and dynamic perfusion culture is conducted in combination with a culture medium containing VEGF-A and FGF-2. And finally, the structure and the function of the vascular organ are verified through immunofluorescent staining and fluorescent microbead perfusion. The problems that in the prior art, cell sources are limited, culture conditions are single and the like are solved, the high angiogenesis potential of the human umbilical artery stem cells and a dynamic culture system are utilized to construct a high-bionic-degree vascular network, and the method can be applied to drug screening, vascular pathological model construction and regenerative medicine research.

The invention discloses a space-time responsive multifunctional 3D printing bone repair scaffold as well as a preparation method and application thereof. PDGF-BB is loaded on a quick-release carrier, MgP is loaded on a slow-release carrier, and the multifunctional 3D printing bone repair scaffold with MgP loaded on the inner layer and PDGF-BB loaded on the outer layer is prepared through 3D printing. Wherein the PDGF-BB is used as an immunomodulator, the MgP is used as a regeneration inducer, and after the carrier is implanted into a body, according to different carrier degradation rates, the PDGF-BB is degraded and released in a space-time responsive manner to realize early immunoregulation and endothelial cell recruitment, and then magnesium ions and phosphate ions are degraded and released to synergistically promote vascularization and osteogenesis in middle and later periods with the PDGF-BB. In-vitro and in-vivo experiments prove that the 3D printing bone repair scaffold provided by the invention has immunoregulation, angiogenesis promotion and osteogenesis properties, can be used for repairing inflammatory bone injury, and is particularly suitable for repairing inflammatory bone injury caused by diabetes.

This invention, entitled "A Method and Product for Pretreatment of Mesenchymal Stem Cells and Its Application in Stroke Drugs," belongs to the field of biotechnology. The technical problem to be solved is to improve the function and therapeutic effect of mesenchymal stem cells. The key technical solution is a method for pretreatment of mesenchymal stem cells, including: culturing cells using an activation medium, culturing cells using a directional medium, and culturing cells under hypoxic conditions using an adaptation medium; the activation medium contains complete medium, IFN-γ, TNF-α, and IL-1β; the directional medium contains complete medium, sodium valproate, 5-azacytidine, and all-trans retinoic acid; the adaptation medium is a complete medium containing basal medium and free of animal serum. The pretreatment method of this invention enhances the anti-inflammatory, neuroprotective, and angiogenic capabilities of mesenchymal stem cells, showing significant therapeutic effects on stroke, and since it does not use animal serum, it meets clinical requirements.

A film for integrating with tissue and promoting at least one of endothelial cell adhesion, migration, proliferation, ingrowth, infiltration, angiogenesis, sprouting angiogenesis, neovascularization, and vascularization includes a plurality of fibers arranged in an overlapping configuration to form a porous, thin film with an average pore size of at least 0.01 μm2 and less than 60 μm2. The plurality of fibers has an average diameter of at least 10 nm and less than 1,000 nm. Each of the plurality of fibers have an aspect ratio of length to width of at least 10, and each of the plurality of fibers is a biocompatible material.

This invention relates to the field of medical materials technology, providing a hydrogel containing antler mesenchymal stem cell exosomes, its preparation method, and applications. The method includes the following steps: preparation of antler mesenchymal stem cell exosomes; preparation of PVP-HA composite hydrogel; uniformly mixing AMSC-Exos with a hydrogel precursor solution, and forming a hydrogel containing antler mesenchymal stem cell exosomes through physical embedding and non-covalent interactions. This invention utilizes the three-dimensional porous network structure of the hydrogel to achieve long-term sustained release of exosomes, protecting them from rapid degradation; it leverages the unique regenerative advantages of antler-derived exosomes to synergistically promote angiogenesis, inhibit inflammatory responses, and regulate macrophage polarization; it promotes angiogenesis through the miR-486-5p / FGF9 axis, achieving efficient repair of chronic diabetic wounds; and it provides a novel, efficient, safe, and clinically translational strategy for the treatment of chronic diabetic skin lesions.

Disclosed is a method for producing a stem cell with enhanced efficacy, a stem cell produced thereby, and a method for treating a disease using the same.According to the present invention, sizes of stem cells may be reduced and proliferation ability, differentiation potency, migration ability, and angiogenic potential of stem cells may be significantly enhanced by applying a culturing method provided herein. Thus the present invention may be usefully applied to development of therapeutic agents using stem cells.

This invention discloses an interpenetrating network antibacterial hydrogel with angiogenesis function, its preparation method, and its applications. The hydrogel is formed by in-situ photocrosslinking of a precursor solution to create a three-dimensional interpenetrating network structure. The precursor solution comprises the following components by mass fraction: 0.5%–1.5% gallic acid-modified low-viscosity chitosan, 0.5%–1.5% decellularized liver matrix, 8%–12% methacrylic acid gelatin, 0.5%–1.0% photoinitiator, and the balance being phosphate-buffered saline. This invention uses low-viscosity chitosan as the modifying unit, combined with a strategy of precise acid adjustment before blending. This not only effectively avoids the risks of polymer phase separation and flocculation that are easily caused during multi-component blending, but also fundamentally solves the problem of the influence of the acidic microenvironment on the interpenetrating crosslinking of polymers. The precursor solution of this hydrogel has excellent fluidity, can precisely adapt to irregular defects, and can solidify rapidly in situ. It integrates antibacterial properties and induces angiogenesis, showing broad application prospects in the repair of complex skin tissues such as chronic diabetic wounds.

The invention discloses hydrogel for osteoporotic bone defect repair and a preparation method of the hydrogel. The hydrogel comprises basic hydrogel and mineralized photosynthetic microorganisms, the mineralized photosynthetic microorganism comprises a photosynthetic microorganism, a mineralized shell layer arranged on the surface of the photosynthetic microorganism, and rare earth up-conversion nano-particles loaded in cells of the photosynthetic microorganism, and the rare earth up-conversion nano-particles can convert near-infrared light into visible light. According to the present invention, the oxygen production type mineralized microalgae composite hydrogel with characteristics of deep tissue activation, controllable oxygen production and simultaneous realization of four-effect synergy of acid neutralization, continuous oxygen supply, angiogenesis promotion and osteogenesis mineralization can be provided, such that osteoblast activity can be promoted, osteoclast activity can be inhibited, in-vivo osteogenesis can be promoted, and the technical support can be provided for osteoporotic bone defect repair.

The invention relates to the technical field of biomedical materials and hemostatic preparations, in particular to a convertible composite powder material and a preparation method and application thereof. The preparation method of the composite powder material comprises the following step: compounding the coenzyme-based polymer PLA / Na and the antibacterial and / or hemostatic material. The material is stable in a dry state, can quickly absorb water within 10 seconds after being in contact with body fluid or blood, and is self-crosslinked to form tough adhesive hydrogel, so that immediate physical sealing of wet and irregular wounds is realized; the material has multiple functions of rapid self-gelling, excellent tissue adhesion, spontaneous wound penetration promotion, efficient hemostasis, broad-spectrum antibacterial, antioxidant, anti-inflammatory, active angiogenesis and tissue regeneration promotion and the like; the material is simple in preparation process, high in biocompatibility and convenient to store and use, and has a wide clinical application prospect in the fields of emergency hemostasis and wound repair.

The invention provides a mesenchymal stem cell exosome for overexpressing WNT3a protein as well as a preparation method and application of the mesenchymal stem cell exosome. The exosome provided by the embodiment of the invention is secreted by the engineered mesenchymal stem cells overexpressing the WNT3a protein, the WNT3a protein exists in the exosome and / or on the membrane, and the WNT3a protein can be efficiently and stably conveyed to the hair papilla cells in a targeted manner by utilizing the natural delivery function of the exosome, so that the effect of treating the hair papilla cells is achieved. Cooperative regulation of the WNT3a protein on multiple factors such as hair papilla cell proliferation, related signal channel activation, angiogenesis and local microinflammation relieving environment is achieved, so that hair follicle regeneration and hair growth are effectively promoted, and a brand-new, efficient and safe strategy is provided for preparing the medicine for treating or preventing alopecia.

This invention discloses a barrier membrane for guiding bone regeneration and its preparation method, relating to the field of materials technology for guiding bone regeneration. The barrier membrane comprises a stacked nanowire functional layer and a barrier layer, wherein the nanowire functional layer includes a Cu-loaded surface. 2+ Sr 2+ The barrier membrane, doped with hydroxyapatite nanowires, comprises polylactic acid as the barrier layer. This invention provides a barrier membrane that combines sequential ion release, directional ion release, immune regulation, angiogenesis promotion, osteoproliferation, and antibacterial adhesion functions, adapting to the stage-specific needs of bone repair and guiding bone regeneration. This invention effectively solves the problems of existing barrier membranes, which only provide physical barrier function, lack biological activity, are difficult to actively promote bone regeneration, have a single mode of active metal ion release, are difficult to achieve sequential regulation, have a high risk of postoperative infection, exhibit disordered diffusion of active components, and have limited functionality.

The invention discloses a PINK1 inducible expression cell line of a porcine umbilical vein endothelial cell and a construction method and application thereof, a stable cell line with controllable PINK1 expression level is constructed by introducing a porcine PINK1 gene into the porcine umbilical vein endothelial cell and combining with an inducible expression system taking doxycycline as an inducer. The cell line is in a low-expression or non-expression state under the condition that an inducer is not added, and dose-dependent induced expression can be realized after doxycycline is added. Experimental results show that the induced expression of PINK1 can regulate and control the expression of angiogenesis related genes, activate mitochondrial autophagy related pathways under oxidative stress conditions, and influence the expression of cell proliferation and apoptosis related proteins at the same time. The cell line is clear in construction method and good in stability, can be used as an in-vitro model for researching angiogenesis, oxidative stress and related regulation and control mechanisms of porcine vascular endothelial cells, and has good practical value.

This invention relates to a mildly photothermal-releasing, injectable, self-healing hydrogel dressing for wound healing after oral cancer surgery. The hydrogel dressing is prepared by loading NO donor-loaded nanoparticles into a thermosensitive ABA triblock copolymer. This hydrogel dressing possesses thermosensitivity, injectability, self-healing properties, mild photothermal antibacterial activity, controlled NO release, blood compatibility, and biocompatibility. It can promote tissue regeneration, angiogenesis, and cell migration in postoperative wounds after oral cancer surgery, making it an ideal material for postoperative wound management in oral cancer patients.

The present invention relates to a hyaluronic acid-based cell spheroid containing iron oxide and a manufacturing method therefor. By introducing hyaluronic acid particles containing magnetic iron oxide and inducing physical stimulation thereto, vascularization and osteogenic differentiation ability of the cell spheroid can be improved.

The invention provides a bionic liposome targeting intervertebral disc nucleus pulposus cells as well as a drug delivery system, a preparation method and application of the bionic liposome, and belongs to the technical field of biological medicine preparation. The invention provides a bionic liposome targeting an intervertebral disc nucleus pulposus cell. The bionic liposome comprises a drug-loaded liposome and a nucleus pulposus cell membrane coated on the surface of the drug-loaded liposome. The drug delivery system is constructed on the basis of the bionic liposome, a Hippo pathway inhibitor or an SNAI1 inhibitor can be accurately and effectively delivered to intervertebral disc nucleus pulposus, in-vivo and in-vitro experiments show that the feasibility of treating intervertebral disc degeneration and pathological vascularization by targeting a YAP-SNAI1-angiogenesis axis is achieved, and a new strategy is provided for clinical treatment.