365 results about "Vasculogenesis" patented technology

The invention provides a preparation method of a mixed pilatory of active peptide and stem cell exosome, and belongs to the technical field of pilatory preparation. The preparation method comprises the following steps: extracting the stem cell exosome; extracting an active extract; preparing an exosome entrapped active extract; preparing the liposome loaded active peptide; preparing the liposome loaded active peptide modified by the gallic acid modified chitosan; and preparing the mixed hair restorer. The prepared mixed hair restorer can provide a suitable growth environment for hair follicles and promote hair growth, the synergistic effect of the active peptide and the exosome can provide abundant signal molecules and enhance the stimulation effect on hair follicle cells, and the active peptide and the exosome can promote angiogenesis and increase the blood flow volume of scalp, so that the hair follicle can be effectively promoted. More nutrition and oxygen are provided for hair follicles, and the growth of hair is effectively promoted.

The invention belongs to the technical field of artificial prosthesis materials, and particularly relates to a medical implant with a lithium disilicate coating and a preparation method thereof, and the preparation method comprises the following steps: (1) placing a prefabricated matrix in a high-temperature environment for staged sintering, and then carrying out surface polishing, cleaning and drying treatment; (2) mixing lithium ethoxide and tetraethoxysilane at room temperature, and reacting for 2-4 hours while stirring to obtain lithium disilicate sol; the preparation method comprises the following steps: preparing a prefabricated substrate, soaking the prefabricated substrate in lithium disilicate sol for 3-7 minutes, performing sol permeation for 3-10 minutes under the negative pressure of more than 30 mm Hg, adding an ethanol water solution to initiate gelation reaction, taking out the prefabricated substrate after the reaction is completed, drying and sintering to obtain the medical implant with the lithium disilicate coating. According to the method, the uniform and continuous lithium disilicate coating which is not easy to fall off can be formed on the surface of the prefabricated matrix of the implant, so that the surface performance of the implant is improved, and the osteogenesis promoting performance and the angiogenesis promoting performance of the implant are optimized.

The invention discloses functional injection type bionic hydrogel for promoting bone healing and a preparation method thereof, and relates to the technical field of medical instruments. The hydrogel has good functional characteristics, is a biomimetic material prepared on the basis of methacrylated gelatin, and aims to regulate and control the degradation rate, the drug release mechanism and diversified synergistic treatment and promote bone repair and regeneration; according to the hydrogel, matching of the degradation rate and the bone regeneration rate is achieved through reasonable matching, stem cell osteogenic differentiation and angiogenesis are promoted, ultrasonic controlled-release drug release and synergistic controlled-release of active factors are achieved, inflammation is reduced, and the bone repair process is promoted; the calcium phosphate bone cement provides biocompatibility and bone conductivity, and the nano clay releases active ions to improve the microenvironment and promote bone repair; in addition, the magnetic nanoparticles and a static magnetic field have synergistic interaction, the bone healing process is further accelerated, and treatment effect monitoring is enhanced through magnetic resonance imaging.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention discloses a filling and repairing agent for promoting angiogenesis and a preparation method thereof, the filling and repairing agent is composed of a vascular endothelial cell supernatant, acellular matrix particles, a suspending aid and a protective agent, the vascular endothelial cell supernatant is prepared by inducing vascular endothelial cells into a tube under a low-oxygen three-dimensional condition, collecting a cell culture solution, centrifuging and concentrating; the acellular matrix particles are obtained by acellular tissue matrix, shearing, freeze-drying, liquid nitrogen grinding and collecting; according to the filling repairing agent for promoting angiogenesis, the vascular endothelial cell supernate and the acellular matrix particles are combined, the filling repairing agent contains high-concentration angiogenesis-related factors and a stable three-dimensional bionic scaffold structure, function complementation is achieved through the synergistic effect of the bioactive factors and the bionic scaffold, and the filling repairing agent for promoting angiogenesis is obtained. Therefore, the filling repair agent which can promote angiogenesis, construct a physical support framework and promote tissue repair and regeneration capacity is innovatively obtained.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant human III-type collagen for promoting angiogenesis as well as a preparation method and application of the recombinant human III-type collagen. The recombinant human III-type collagen for promoting angiogenesis has an amino acid sequence as shown in SEQ ID NO. 1. Nine different sites of the full-length natural III-type collagen are replaced by GFOGER sequences which are from the I-type collagen and are specifically combined with integrin sites, the angiogenesis promoting function of the collagen is enhanced under the condition that the total length of natural full-length amino acid is not changed, and meanwhile, four natural integrin sites with low adhesion rate are also replaced; the recombinant III-type collagen has high adhesive force, so that angiogenesis is further promoted. By means of the transformation mode, the angiogenesis rate is increased by 37% or above, the molecular weight of the translated protein is moderate, and the translated protein is easy to prepare.

The invention discloses a preparation method and application of a response type NO release anticoagulant hydrogel material. The preparation method comprises the following steps: providing methylacryloylated recombinant collagen; providing methacrylated hyaluronic acid; providing caffeic acid arginine amide modified hyaluronic acid; the response type NO release anticoagulant hydrogel material is obtained by taking methylacryloylated recombinant collagen, methylacryloylated hyaluronic acid and caffeic acid arginine amide modified hyaluronic acid as raw materials. The prepared hydrogel material has the advantages of resisting bacteria, promoting proliferation of endothelial cells, inhibiting proliferation of smooth muscle cells, resisting inflammation, promoting angiogenesis, inhibiting thrombosis and the like, arginine can serve as an endogenous NO donor in blood to release NO molecules, and therefore the effect of promoting vascular endothelial repair is achieved, and the hydrogel material has the advantages of being capable of resisting bacteria, promoting proliferation of endothelial cells, inhibiting proliferation of smooth muscle cells, resisting inflammation, promoting angiogenesis, inhibiting thrombosis and the like. The introduced NO molecules enable the hydrogel material to have good anticoagulant performance, and the hydrogel material is expected to be used for preparing an artificial blood vessel anticoagulant coating.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention provides an endometrial mesenchymal stem cell-derived exosome and application thereof in preparation of a medicine for treating diabetic wounds, and belongs to the technical field of biological medicines. The endometrial mesenchymal stem cell-derived exosome provided by the invention can effectively promote HUVEC tube forming ability, promote wound angiogenesis of diabetic model mice, increase collagen deposition and reduce wound area, finally achieves the purpose of promoting healing of diabetic wounds, and solves the problem of limited treatment effect of exosomes in the prior art. The endometrial mesenchymal stem cell-derived exosome provided by the invention can be used as a medicine for promoting angiogenesis and wound healing to be applied to diabetes wound treatment, and also provides a new strategy for treatment of other chronic wounds including burn.

The invention discloses a method for promoting diabetic fracture healing by using bone marrow mesenchymal stem cell exosomes, which comprises the following steps: separating MSCs (mesenchymal stem cells) from the bone marrow of a diabetic patient, culturing and extracting the exosomes; the method comprises the following steps: extracting an exosome from a bone fracture part, performing quantitative analysis on the extracted exosome, injecting the exosome into the bone fracture part in a manner of intravenous injection, local injection or minimally invasive injection, continuously treating, promoting proliferation and differentiation of osteoblasts by miRNA or mRNA in the exosome through targeting cells, enhancing angiogenesis, and further accelerating fracture healing. According to the exosome, proliferation and differentiation of osteoblasts are promoted through miRNA or mRNA in the exosome, the synthesis capacity of bone matrixes of the osteoblasts is enhanced, so that fracture healing is accelerated, meanwhile, the exosome provides necessary blood supply for fracture healing by adjusting vascular endothelial cells and promoting angiogenesis, and the repair capacity of fracture parts is effectively improved.

The invention discloses a nano-enzyme microneedle system for remodeling a diabetes wound microenvironment to promote healing and a preparation method of the nano-enzyme microneedle system. The nano-enzyme microneedle system comprises nano-enzyme and a microneedle carrier, the nano-enzyme is prepared by a self-assembly method and is loaded in the microneedle carrier; the microneedle carrier is composed of a hydrogel matrix and is molded into a microneedle array through 3D printing; according to the system, the nano-enzyme can be delivered to the corium layer by penetrating the cuticle of the skin through the microneedle. The traditional Chinese medicine composition has the advantages that ROS (reactive oxygen species) is efficiently removed, oxidative stress injury induced by high glucose (HG) is relieved, immune cell polarization is regulated, inflammatory factor release is regulated and controlled, the chronic inflammation state of wound surfaces is relieved, cell proliferation and migration are promoted, neovascularization is accelerated, the healing speed of the diabetic wound surfaces is obviously increased, inflammation infiltration is reduced, granulation tissue formation and collagen deposition are obviously improved, and the effect of treating diabetes mellitus is achieved. And no systemic toxicity is observed. Through the synergistic effect of resisting oxidation, resisting inflammation and promoting angiogenesis, the pathological microenvironment of the diabetic wound is effectively improved.

The invention discloses an antibacterial dressing for promoting wound repair and regeneration and a preparation method thereof, and the preparation method comprises the following steps: adding hydrogen peroxide into a silk fibroin aqueous solution to prepare a silk fibroin aqueous solution premixed with hydrogen peroxide; horse radish peroxidase is added into the dopamine modified sodium alginate solution, and the dopamine modified sodium alginate solution premixed with horse radish peroxidase is prepared. Preparing a chondroitin sulfate composite solution; uniformly mixing the silk fibroin aqueous solution premixed with hydrogen peroxide, the dopamine modified sodium alginate solution premixed with horse radish peroxidase and the chondroitin sulfate composite solution. Hydrogel formed by cross-linking silk fibroin and chondroitin sulfate is used as a main body material of the dressing, and meanwhile, dopamine modified sodium alginate is introduced, so that the dressing has the photo-thermal antibacterial characteristic; magnesium oxide microspheres are introduced as a pore-foaming agent, micropores are formed in the dressing, and the liquid absorption property and the air permeability of the dressing are improved; the umbilical cord mesenchymal stem cell exosome is introduced, so that angiogenesis at the wound is promoted, and the wound healing promoting capability of the dressing is improved.

The invention discloses a stem cell preparation for treating pulmonary fibrosis. The stem cell preparation is mesenchymal stem cell surface modified nanoparticles. The nanoparticles are ROS (reactive oxygen species) response type lipid polymer hybrid nanoparticles loaded with an anti-fibrosis drug. According to the invention, a hydrophobic drug and a hydrophilic anti-fibrosis drug can be simultaneously loaded through the reductive hybrid nanoparticles, and can be delivered to the lung by utilizing the inflammation targeting capability of stem cells. The polymer in the nanoparticles reacts with active oxygen in the microenvironment to promote drug release, and then the effect of protecting stem cells is achieved. The released medicine can induce dedifferentiation of myofibroblasts and inhibit apoptosis of vascular endothelial cells and endothelial mesenchymal transformation, and the mesenchymal stem cells release growth factors at the same time to promote angiogenesis, reconstruct capillary structures deleted in fibrosis areas, promote lung tissue regeneration and recover lung functions to achieve the effect of treating pulmonary fibrosis.

The invention belongs to the field of biological medicine, and discloses a preparation method and application of a mesenchymal stem cell-derived exosome entrapped valproic acid compound. According to the invention, the mesenchymal stem cell source exosome is used as a carrier, valproic acid is entrapped, and the compound of VPA entrapped by the exosome of MSCs is prepared by an ultrasonic method, so that the VPA-EXO prepared by the invention is proved to be capable of promoting faster repair of the wound surface by inhibiting inflammation, promoting collagen formation, accelerating angiogenesis and reepithelization of the wound surface. The valproic acid-loaded human umbilical cord mesenchymal stem cell exosome prepared by the invention is VPA-EXO prepared by an ultrasonic method, has no obvious toxicity to cells, and has obvious advantages.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The present invention provides ophthalmic formulations having high concentrations of vascular endothelial growth factor (VEGF) receptor fusion protein and high stability during storage. Methods for treating angiogenic eye disorders using the high concentration formulations are also provided.

According to the multifunctional conductive injection hydrogel OQG provided by the invention, through dynamic Schiff base crosslinking between oxidized dextran (Dextran) and quaternary ammonium salt chitosan (QCS), enhancement by using polydopamine modified reduced graphene oxide (rGO (at) PDA) and loading of polydopamine encapsulated asiaticoside (AC (at) PDA), the obtained hydrogel has injectability, is suitable for irregular wounds, can be quickly self-healed, and has the advantages of good biocompatibility and good biocompatibility. And the wound adhesive force is relatively good. In addition, the composite hydrogel also has antioxidant and antibacterial activity, and can relieve inflammation and prevent infection. The incorporation of conductive reduced graphene oxide further promotes the delivery of endogenous electrical signals, stimulates cell migration and tissue regeneration, while also maintaining the release of AC to modulate collagen deposition and angiogenesis. In-vitro evaluation proves that the hydrogel disclosed by the invention has cell compatibility for promoting cell migration. Rat wound assessment results show that the multifunctional hydrogel can accelerate wound healing, reduce inflammation and reduce scars.

The invention discloses a rapidly-separated microneedle patch as well as a preparation method and application thereof. The microneedle patch comprises a needle tip prepared from a needle tip liquid and a separation backing prepared from a separation substrate liquid, the needle tip liquid is composed of a drug-containing clathrate compound solution, a photoinitiator and a hypotonic agent; the drug-containing clathrate compound is prepared by covalently connecting a beta-cyclodextrin conjugated polymer material with an angiogenesis promoting drug and then clathrating a 5alpha-reductase inhibitor, the separation substrate liquid is composed of a polymer material, a gas initiator and a solvent; the gas initiator is composed of an acid source and an alkali source; the rapid separation microneedle is high in separation and permeation promotion performance, the hair follicle microenvironment is improved from multiple aspects, hair regeneration is promoted, and the rapid separation microneedle has wide application prospects in treatment of androgen alopecia.

The invention discloses a microneedle patch loaded with vancomycin and a gold / zinc cerium composite nanomaterial and application of the microneedle patch in abscess wound treatment, the microneedle patch comprises a backing layer and a needle body array arranged on the backing layer, the backing layer and the needle body array are formed by an aqueous solution of a degradable polymer, vancomycin and a gold / zinc cerium composite nano material are loaded in an aqueous solution of the degradable polymer for forming the needle body array. According to the microneedle patch disclosed by the invention, based on the photo-thermal synergistic effect of the antibiotics and the gold / zinc-cerium composite nanomaterial, rapid healing of abscess wounds is realized from the aspects of inhibiting growth of bacteria, relieving oxidative stress, promoting angiogenesis and the like.

The invention belongs to the technical field of microfluidic organ chips, and particularly relates to an anti-cancer drug screening chip and method based on cancer cell migration and angiogenesis, the organ chip comprises at least one monitoring culture module, and the monitoring culture module comprises a vascular endothelial cell culture layer, a PET porous membrane layer and a tumor cell culture layer which are fixedly connected in sequence; the vascular endothelial cell culture layer comprises a vascular endothelial cell culture chamber, and two sides of the vascular endothelial cell culture chamber are respectively communicated with a liquid inlet I and a liquid outlet I; the tumor cell culture layer comprises a tumor cell culture chamber, one side of the tumor cell culture chamber is communicated with a liquid inlet II, the tumor cell culture chamber and the vascular endothelial cell culture chamber are correspondingly arranged and communicated, and the PET porous membrane layer is arranged between the vascular endothelial cell culture chamber and the tumor cell culture chamber; one side of the migration chamber is communicated with the other side of the tumor cell culture chamber, and the other side of the migration chamber is communicated with a liquid outlet II.

The invention discloses a chiral supramolecular fiber structure design based on glutamic acid derivative and pyrazine co-assembly, a preparation method and application in bone regeneration and vascular regeneration. The chiral supramolecular assembly disclosed by the invention is formed by co-assembling a glutamic acid derivative and pyrazine, is an all-organic three-dimensional spiral fiber material with an accurate and controllable three-dimensional topological configuration, and can realize coupling regeneration of bones and blood vessels by cooperatively regulating and controlling dual action mechanisms of osteogenic differentiation and angiogenesis; the compound can be used for efficiently treating complex bone defects and related bone diseases. In addition, raw materials and reagents are simple and easy to obtain, reaction conditions are mild, the preparation method is simple, and good application prospects are achieved.

The invention relates to the technical field of biology, in particular to a method for preparing a mesenchymal stem cell exosome with a cancer cell inhibiting effect. According to the application, bioactive substances such as various growth factors, cell factors and exosomes which are rich in the stem cell conditioned culture medium are utilized, and the substances jointly act on cancer cells through various mechanisms such as inducing cell apoptosis, blocking a cell cycle and inhibiting tumor angiogenesis, so that a multi-layer anti-cancer effect is formed; especially, the addition of CBD further enriches or optimizes exosomes in the culture medium, the exosomes may carry more molecules with anticancer characteristics under the induction of CBD, the potential inhibition effect on cancer cells is significantly enhanced, and in addition, the stem cell conditioned culture medium as a treatment tool has a significant treatment effect on cancer cells. Especially, cannabidiol with relatively low cost is combined for treatment, a new thought is provided for developing a cancer treatment strategy with reasonable cost, and the method is expected to reduce the overall treatment cost, so that more patients are benefited.

The invention aims to provide a preparation method and application of a nano-enzyme hydrogel dressing, and belongs to the technical field of wound repair materials, the method prepares a nano-enzyme Mn-ZIF (at) GOx which is composed of manganese and zinc doped MOF and has multi-enzyme activity, and the nano-enzyme Mn-ZIF (at) GOx is anchored on bacterial cellulose hydrogel. Mn-ZIF (at) GOx / BC shows an antibacterial property independent of ROS (reactive oxygen species) and a relatively strong immunoregulation characteristic. According to the hydrogel, by simulating the cascade process of superoxide dismutase and catalase, hydroxyl free radicals, superoxide anions and hydrogen peroxide are effectively removed, O2 is generated, and M2 polarization of macrophages can be induced, so that inflammation is relieved. MZGB creates a good immune microenvironment through reprogramming macrophages, and proliferation, migration and angiogenesis of fibroblasts and endothelial cells are remarkably promoted through a paracrine mechanism. The MZGB hydrogel disclosed by the invention has the characteristics of resisting inflammation, removing active oxygen, resisting bacteria and promoting angiogenesis, obviously enhances the healing of diabetic wounds, and is simple to prepare.

The invention belongs to the technical field of preparation of nano functional materials, and provides a difunctional nanosheet as well as a preparation method and application thereof. On the basis of two-dimensional layered double-metal oxides (LDHs), magnesium-aluminum hydrotalcite (MgAl-LDH) is prepared as a main body laminate, and bioactive components Ce < 3 + > and Sr < 2 + > are introduced into the main body laminate, so that the bifunctional nanosheet capable of accurately and efficiently removing ROS and promoting angiogenesis is developed; wherein due to the existence of Ce < 3 + > and Sr < 2 + >, the nano-sheet has the activity of superoxide dismutase and the characteristic of promoting vascular regeneration at the same time, active sites fully exposed on the surface of the nano-sheet can effectively remove ROS and regulate an anoxic microenvironment, meanwhile, the nano-sheet responds to an ischemic acid microenvironment to release Sr < 2 + >, proliferation and migration of endothelial cells are promoted, and angiogenesis is facilitated. The MgSrCeAl-LDH nanosheet disclosed by the invention is used for treating the MIRI, and the treatment effect is remarkable.

A cage type support for bone grafting capable of achieving heat balance comprises a support shell and a base, and a multipurpose cavity is formed in the base. The inner cavity of the bracket shell is communicated with the multi-purpose chamber through a heat convection pipe group; the multipurpose cavity is configured to be a heat dissipation end communicated with the inner cavity, and heat balance of the inner cavity and the multipurpose cavity is achieved through a heat convection pipe set communicated between the multipurpose cavity and the inner cavity; a stable supporting foundation is provided, the osseointegration process is promoted, heat balance of the whole stent is achieved, the internal temperature of the femoral head is properly increased, local blood circulation of the femoral head is accelerated, cell activity is enhanced, metabolism is accelerated, proliferation and differentiation of related cells such as bone cells are promoted, angiogenesis and bone repair are promoted, and gas exchange is facilitated. The implant can be used for promoting the discharge of hydrops and pneumatosis, continuously releasing intraosseous pressure and providing a storage space and path for therapeutic drugs and / or nutritional ingredients, and the implant realizes multi-system collaboration and promotes overall rehabilitation.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.

The invention relates to the technical field of biological medicine, in particular to a cerium oxide nano-enzyme delivery system based on carried neutrophil, and the system comprises cerium oxide nano-particles; the surfaces of the cerium oxide nanoparticles are coated with the lipoic acid zwitterionic polymer layers; the sialic acid molecules and the protocatechuic aldehyde molecules are modified on the surface of the lipoic acid zwitterionic polymer layer; wherein the system realizes breakthrough of a blood brain barrier and precise delivery of ischemic lesions through mediation targeting delivery of L-selectin on the surface of neutrophile granulocyte, and based on a cerium oxide nano-enzyme delivery system carrying neutrophile granulocyte, a'antioxidation-anti-inflammation-angiogenesis promotion-neural restoration 'quadruple synergistic mechanism is integrated, so that the system has the advantages that the system is simple in structure and convenient to use, and the application prospect is wide. And a new strategy of multi-target intervention is provided for cerebral arterial thrombosis treatment.

The invention discloses a titanium-magnesium hollow screw system capable of promoting fracture healing and a manufacturing method. The titanium-magnesium hollow screw system comprises a titanium-based supporting structure and a magnesium filling layer. The titanium-based supporting structure comprises a main body part, the surface of the main body part is provided with an imitated bone trabecula part, and a plurality of bone gaps are formed in the imitated bone trabecula part; wherein bone gaps are filled with the magnesium filling layer, and the magnesium filling layer is combined with the titanium-based supporting structure in a physical embedding mode; the titanium-based supporting structure bears and maintains initial fixation and long-term bearing, so that the strength and the stability of the screw are ensured; the surface imitated bone trabecula and the communicated bone gaps increase the surface area and provide a physical channel for vascularization and bone tissue ingrowth, so that synostosis is promoted; the magnesium filling layer releases magnesium ions after being locally controllably degraded, so that osteogenesis and angiogenesis are favorably stimulated, the local biological environment is improved, instant mechanical support after an operation is ensured, bone healing is promoted through local biological activity release and structural orientation, the healing time is favorably shortened, and the healing quality is favorably improved.

The invention discloses a black phosphorus hydrogel microneedle patch loaded with trained mesenchymal stem cells as well as a preparation method and application of the black phosphorus hydrogel microneedle patch, and belongs to the technical field of biological medicines. The micro-needle patch comprises a hydrogel substrate layer loaded with black phosphorus nanosheets and a hydrogel needle tip part for packaging the stem cells. The mesenchymal stem cells are subjected to combined domestication with low oxygen and high active oxygen, so that the anti-oxidative stress, anti-inflammatory and angiogenesis promoting capabilities of the mesenchymal stem cells are remarkably enhanced. After the patch is subcutaneously delivered, the black phosphorus component can efficiently remove bacteria and reduce drug resistance; after being domesticated, the stem cells can adapt to the immune microenvironment of the wound surface, and the immune regulation, inflammation inhibition and tissue regeneration promotion are synergistically realized by continuously releasing anti-inflammatory and angiogenesis promoting factors, so that the refractory wound surface caused by immune disorders such as systemic lupus erythematosus (SLE) and the like is effectively treated. The invention provides an innovative treatment strategy for chronic inflammatory wound healing.