37 results about "Vasculogenesis" patented technology

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The application discloses a wound dressing and a preparation method and application thereof; the wound dressing comprises polymer flexible fibers and cobalt-iron layered double hydroxide nanosheets dispersed in the polymer flexible fibers; the cobalt-iron layered double hydroxide nanosheets contain a cobalt vacancy structure in a crystal lattice. The wound dressing has a strong "electron capture effect" due to the cobalt vacancy. The wound dressing can physically capture electrons on a respiratory transmission chain of a pathogenic bacterial cell membrane, interferes with transmembrane proton transfer and ATP synthesis, directly kills the bacteria through "metabolic starvation", and further has a broad-spectrum physical bactericidal function independent of antibiotics. The wound dressing has excellent superoxide dismutase-like and catalase-like activities, can long-acting capture and remove active oxygen such as superoxide anions and hydrogen peroxide in an inflammatory microenvironment of a wound, promotes macrophages to polarize to a M2 phenotype for promoting repair, and has anti-inflammatory and pro-angiogenic functions.

This invention, entitled "A Method and Product for Pretreatment of Mesenchymal Stem Cells and Its Application in Stroke Drugs," belongs to the field of biotechnology. The technical problem to be solved is to improve the function and therapeutic effect of mesenchymal stem cells. The key technical solution is a method for pretreatment of mesenchymal stem cells, including: culturing cells using an activation medium, culturing cells using a directional medium, and culturing cells under hypoxic conditions using an adaptation medium; the activation medium contains complete medium, IFN-γ, TNF-α, and IL-1β; the directional medium contains complete medium, sodium valproate, 5-azacytidine, and all-trans retinoic acid; the adaptation medium is a complete medium containing basal medium and free of animal serum. The pretreatment method of this invention enhances the anti-inflammatory, neuroprotective, and angiogenic capabilities of mesenchymal stem cells, showing significant therapeutic effects on stroke, and since it does not use animal serum, it meets clinical requirements.

This invention relates to the field of medical materials technology, providing a hydrogel containing antler mesenchymal stem cell exosomes, its preparation method, and applications. The method includes the following steps: preparation of antler mesenchymal stem cell exosomes; preparation of PVP-HA composite hydrogel; uniformly mixing AMSC-Exos with a hydrogel precursor solution, and forming a hydrogel containing antler mesenchymal stem cell exosomes through physical embedding and non-covalent interactions. This invention utilizes the three-dimensional porous network structure of the hydrogel to achieve long-term sustained release of exosomes, protecting them from rapid degradation; it leverages the unique regenerative advantages of antler-derived exosomes to synergistically promote angiogenesis, inhibit inflammatory responses, and regulate macrophage polarization; it promotes angiogenesis through the miR-486-5p / FGF9 axis, achieving efficient repair of chronic diabetic wounds; and it provides a novel, efficient, safe, and clinically translational strategy for the treatment of chronic diabetic skin lesions.

This invention discloses an interpenetrating network antibacterial hydrogel with angiogenesis function, its preparation method, and its applications. The hydrogel is formed by in-situ photocrosslinking of a precursor solution to create a three-dimensional interpenetrating network structure. The precursor solution comprises the following components by mass fraction: 0.5%–1.5% gallic acid-modified low-viscosity chitosan, 0.5%–1.5% decellularized liver matrix, 8%–12% methacrylic acid gelatin, 0.5%–1.0% photoinitiator, and the balance being phosphate-buffered saline. This invention uses low-viscosity chitosan as the modifying unit, combined with a strategy of precise acid adjustment before blending. This not only effectively avoids the risks of polymer phase separation and flocculation that are easily caused during multi-component blending, but also fundamentally solves the problem of the influence of the acidic microenvironment on the interpenetrating crosslinking of polymers. The precursor solution of this hydrogel has excellent fluidity, can precisely adapt to irregular defects, and can solidify rapidly in situ. It integrates antibacterial properties and induces angiogenesis, showing broad application prospects in the repair of complex skin tissues such as chronic diabetic wounds.

This invention discloses a barrier membrane for guiding bone regeneration and its preparation method, relating to the field of materials technology for guiding bone regeneration. The barrier membrane comprises a stacked nanowire functional layer and a barrier layer, wherein the nanowire functional layer includes a Cu-loaded surface. 2+ Sr 2+ The barrier membrane, doped with hydroxyapatite nanowires, comprises polylactic acid as the barrier layer. This invention provides a barrier membrane that combines sequential ion release, directional ion release, immune regulation, angiogenesis promotion, osteoproliferation, and antibacterial adhesion functions, adapting to the stage-specific needs of bone repair and guiding bone regeneration. This invention effectively solves the problems of existing barrier membranes, which only provide physical barrier function, lack biological activity, are difficult to actively promote bone regeneration, have a single mode of active metal ion release, are difficult to achieve sequential regulation, have a high risk of postoperative infection, exhibit disordered diffusion of active components, and have limited functionality.

This invention relates to a mildly photothermal-releasing, injectable, self-healing hydrogel dressing for wound healing after oral cancer surgery. The hydrogel dressing is prepared by loading NO donor-loaded nanoparticles into a thermosensitive ABA triblock copolymer. This hydrogel dressing possesses thermosensitivity, injectability, self-healing properties, mild photothermal antibacterial activity, controlled NO release, blood compatibility, and biocompatibility. It can promote tissue regeneration, angiogenesis, and cell migration in postoperative wounds after oral cancer surgery, making it an ideal material for postoperative wound management in oral cancer patients.

The present invention relates to a hyaluronic acid-based cell spheroid containing iron oxide and a manufacturing method therefor. By introducing hyaluronic acid particles containing magnetic iron oxide and inducing physical stimulation thereto, vascularization and osteogenic differentiation ability of the cell spheroid can be improved.

The application discloses a multifunctional hydrogel patch, a preparation method and application thereof, and belongs to the field of tissue engineering and regenerative medicine. The biological patch comprises a decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs). The dFCT is obtained by decellularization and freeze-drying treatment of tumor tissue, and retains abundant natural extracellular matrix components. The HNPs are extracted from hair and have excellent active oxygen scavenging capacity and photothermal conversion performance. The hydrogel layer is preferably methacrylated gelatin (GelMA). The patch shows significant cell activity promotion and antibacterial capacity in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch has the advantages of simple preparation process, natural component source, multiple functions such as antibacterial, antioxidant and tissue regeneration promotion, and is suitable for wound repair treatment.

This application relates to the field of medical device technology, providing a programmable shape memory bone screw, its preparation method, and its application. The programmable shape memory bone screw comprises a screw body made of shape memory polymer material, the surface of which is coated with a polydopamine layer, and the surface of the polydopamine layer is coated with a calcium ion-arginine composite coating. The provided bone screw possesses superior mechanical and shape memory properties; it can utilize its shape memory function to provide mechanical stress stimulation to bone defect tissue, thereby activating mechanically sensitive calcium ion channels, inducing the influx of calcium ions and arginine from the coating into cells, thereby upregulating the NO-cGMP pathway, synchronously coordinating osteogenic and angiogenesis, and accelerating bone repair; it has an osteogenic-angiogenic coupling function, which can enhance and accelerate bone regeneration, exhibiting excellent biomechanical properties and synchronously coordinating osteogenic and angiogenesis during the bone repair process.

A thermally balanced filler bone graft scaffold includes a scaffold shell and a base. The scaffold shell has an inner cavity. The base has a multi-purpose chamber at its tail end and a heat convection tube assembly at its head end. The multi-purpose chamber is connected to the surface of the head end of the base via the heat convection tube assembly. The heat convection tube assembly connects the inner cavity of the scaffold shell with the multi-purpose chamber of the base. This provides a stable support base, promotes the bone integration process, achieves thermal balance of the overall scaffold, appropriately increases the internal temperature of the femoral head, accelerates local blood circulation in the femoral head, enhances cell activity, accelerates metabolism, promotes the proliferation and differentiation of osteocytes and other related cells, promotes angiogenesis and bone repair, facilitates gas exchange, promotes the drainage of effusion and gas, continuously releases intraosseous pressure, and provides storage space and pathways for therapeutic drugs and / or nutrients. This implant achieves multi-system synergy and promotes overall rehabilitation.

This invention relates to the field of biomedicine and provides a fullerene derivative that inhibits VEGFR2. Specifically, this specification provides the use of the fullerene derivative of formula (I) in the preparation of vascular endothelial growth factor inhibitors and drugs that inhibit angiogenesis, wherein the definitions of each group are detailed in the specification. The fullerene derivative has a strong affinity for biological membrane structures, is easily taken up by cells, and can inhibit angiogenesis by binding to vascular endothelial growth factor.

This invention belongs to the fields of biomedicine and molecular biology, specifically relating to the application of H2AC19 in the detection and treatment of lung adenocarcinoma. Specifically, this invention demonstrates that H2AC19 is abnormally highly expressed in lung adenocarcinoma patients, and this high expression is significantly associated with poor prognosis. Knockout of H2AC19 inhibits angiogenesis and tumor growth in vitro and in vivo, and reduces the proliferation, migration, and tube formation capabilities of lung adenocarcinoma cells in a co-culture model with human umbilical vein endothelial cells. Furthermore, this invention reveals a novel mechanism by which H2AC19 regulates angiogenesis through the p300 / EGR1 / MMP-1 pathway. This invention not only provides a superior method for the diagnosis and prognostic assessment of lung adenocarcinoma, but also lays an experimental foundation and expands new perspectives for the development of highly effective drugs for treating lung adenocarcinoma, thus possessing significant practical application value.

The application discloses a kind of tumor angiogenesis simulation methods, including the blood vessel network topology including environment pixel point and blood vessel pixel point generated based on the medical image of patient;Based on the average path length and step length adjustment factor, chemotaxis weight, bifurcation number range and bifurcation probability of related data such as blood vessel tip of blood vessel network topology are constantly updated;And the bifurcation number of each iteration blood vessel tip and the branch length of each branch after bifurcation are calculated based on the update result of each iteration, and the update of blood vessel network topology is completed based on the bifurcation number and the branch length of each branch after bifurcation calculated, constantly circulate until reaching simulation condition.The application quantifies the transport efficiency of blood vessel network by global function index average path length, realizes the evaluation whether blood vessel network presents efficient local characteristics by clustering coefficient and average path length, guarantees that the blood vessel network obtained by simulation is same with real network.

In particular, compositions comprising iPSC-derived parietal cell precursors (iMPCs) generated using NK3 homeobox 1 (NKX3.1; parietal cell fate-determining transcription factor) and methods for their preparation and use are described herein. In addition, in particular, methods for maturing iMPCs into functional parietal cell subtypes, including smooth muscle cells, perivascular cells, and fibroblasts, as well as methods for increasing angiogenesis, angiogenesis, and cell junctions, are described herein. iMPCs mediate the formation of functional blood vessels when transplanted together with endothelial cells (ECs); thus, methods for modeling vascular diseases (e.g., 3D vascular organoids (VOs)) and therapeutic vascularization, comprising a method of administering iMPCs and ECs and a step of administering iMPCs and ECs, are also described herein.

The present application relates to the technical field of biomedical materials, and particularly relates to copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles and preparation and application thereof.The copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles of the present application take tetragonal phase barium titanate as a core, an oxygen defect amorphous layer as a shell, and a heterojunction is formed by depositing a non-valent copper nanocluster (Cu-Cu + ) on the surface of the shell layer; the copper / oxygen defect barium titanate piezoelectric heterojunction nanoparticles have an "electronic lever" effect, can be used for wound repair treatment by precisely regulating the "antibacterial-promoting repair" time sequence treatment under the action of ultrasound, and can rapidly and massively produce ROS and release copper ions under the action of ultrasound activation, so as to induce copper death of bacteria and achieve efficient antibacterial effect. Then, in the tissue repair stage, the ultrasound is turned off, and the intrinsic electric field of the heterojunction and the release of a small amount of copper ions are utilized to promote granulation tissue regeneration, collagen deposition, epithelial regeneration and angiogenesis, so as to synergistically accelerate tissue repair.

This invention belongs to the field of biomaterials technology, specifically relating to a bioactive scaffold, its preparation method, and its applications. This invention incorporates functionalized mesoporous silica nanoparticles loaded with aminoguanidine into the skin secretions of the giant salamander and polycaprolactone electrospun fibers to construct a bioactive composite scaffold (SPMA). This scaffold enables the sustained release of AG and osteogenic factors, effectively inhibiting free radical generation, AGEs toxicity, and inflammatory responses. Furthermore, it significantly improves the survival rate, osteogenic differentiation, and mineralization capacity of bone marrow mesenchymal stem cells in a high-glucose microenvironment. In vivo experiments further demonstrate that in a streptozotocin-induced diabetic mouse model of skull defects, the SPMA scaffold can alleviate local inflammation, promote angiogenesis, and significantly accelerate new bone formation and defect repair.

The application discloses a preparation method of a 3D printing cerium hydroxyapatite bone repair scaffold material, and comprises the following steps: adding phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate salt powder into manganese-chelated deferoxamine grafted methylacryl gelatin, stirring, adding cerium hydroxyapatite nanowires which are surface-modified by citric acid, and stirring to obtain 3D printing slurry; and the 3D printing slurry is extruded by a 3D printer while being cross-linked by 365nm ultraviolet irradiation to obtain the 3D printing cerium hydroxyapatite bone repair scaffold material; the material has good biocompatibility, can promote vascularization and immune regulation, and can effectively regulate M2 phenotype differentiation of RAW264.7, so that more BMP-2 and PDGF-bb growth factors are secreted, osteogenesis differentiation and angiogenesis are promoted, and bone defect repair is promoted from the perspective of immune regulation.

The application discloses a preparation method of bone organ-derived extracellular vesicles and application of the bone organ-derived extracellular vesicles in promoting angiogenesis. The application comprises the following steps: culturing bone marrow mesenchymal stem cells after osteogenic induction in a three-dimensional culture to construct bone organs, transfecting Hif-1 alpha plasmid, collecting bone organ culture supernatants of different culture time, separating and extracting extracellular vesicles through ultracentrifugation to obtain bone organ-derived extracellular vesicles (BOEVs) rich in BMP-2, TGF-beta 1, HIF-1 alpha and VEGF. The BOEVs are co-cultured with HUVEC cells, and the results show that the BOEVs can significantly promote the lumen formation and branching of the HUVEC cells by about 5 times, and up-regulate the expression of angiogenesis-related genes VEGF and CCN2, indicating that the BOEVs can promote angiogenesis.

The application discloses an NGR peptide coupled ruthenium complex and a preparation method and application thereof, and belongs to the technical field of tumor photodynamic therapy. The application couples NGR peptide and a two-photon active ruthenium complex to construct a double-targeted photosensitizer. The NGR oligopeptide can specifically combine with CD13 receptors which are highly expressed on the surface of tumor vascular endothelial cells and tumor cells, realize double targeting of'vessel+tumor cell', and significantly improve the tumor enrichment efficiency of drugs. The NGR peptide coupled ruthenium complex provided by the application can efficiently generate active oxygen under the excitation of 820 nm near-infrared light, specifically kill CD13 positive target cells, and the tissue penetration depth can reach 250 microns, which is suitable for deep tumor treatment. Meanwhile, the NGR peptide coupled ruthenium complex can efficiently destroy the pipe forming ability of vascular endothelial cells, and significantly inhibit tumor angiogenesis.

The present application provides a kind of CD47 mutant and its application, wherein CD47 mutant includes the amino acid sequence of partial amino acid sequence of CD47 and glycosyl phosphatidyl inositol (GPI) connection signal.The CD47 mutant is obtained by removing potential cell survival inhibition, blood flow and angiogenesis inhibition and other negative effect signal transduction sequences (transmembrane region and intracellular region) in CD47, while the beneficial functional segment (extracellular IgV domain) involved in inhibiting the phagocytosis of myeloid cells and its cytotoxic activity in CD47 sequence is fused with GPI membrane anchor connection signal sequence from human decay accelerating factor (DAF) protein.The obtained CD47 mutant can protect cells and organ transplants from being mediated by myeloid cells and other SIRP alpha positive immune cells with equal efficacy to wild type CD47, and at the same time eliminates the negative effects of wild type CD47.

A kind of cell composition, including the cell cluster consisting of adipose-derived stem cells, endothelial cells and fibroblasts, as fat tissue regeneration unit.It is proved that the cell cluster composition of the application not only promotes the generation of fat, but also makes the generation of fat and angiogenesis and collagen formation etc.parallel, effectively promote the ability of fat tissue regeneration, while solving the problem of survival rate and insufficient vascularization after ADSCs transplantation.The combination of fat tissue regeneration unit and pGelMA not only solves the problem of vascularized fat tissue regeneration, but also promotes the formation of tissue similar to natural mammary tissue, providing a solution for realizing clinical breast reconstruction.

The application relates to the technical field of biology, and particularly discloses an artificial synthetic dermis loaded with subacromial synovial mesenchymal stem cell composite patch as well as a preparation method and application thereof. The artificial synthetic dermis loaded with subacromial synovial mesenchymal stem cell composite patch has the advantages of convenient raw material source, no immune rejection, no supply area complications, no disease transmission risk and the like. Moreover, the composite patch provides initial mechanical support, and improves the tendon-bone interface microenvironment through the paracrine effect (including pro-angiogenic related factors) of the subacromial synovial mesenchymal stem cells, promotes host cell infiltration and blood vessel ingrowth, and enhances tissue integration and rotator cuff healing.

This invention discloses a wound repair gel containing *Pseudolarix amabilis* oil, its preparation method, and its application, belonging to the field of gel technology. The wound repair gel comprises *Pseudolarix amabilis* oil, hyaluronic acid, chitosan, antimicrobial peptides, and a gel matrix, wherein the gel matrix is ​​carbomer and xanthan gum. The preparation method includes first swelling and neutralizing carbomer and xanthan gum to form a gel matrix, then adding *Pseudolarix amabilis* oil, hyaluronic acid, chitosan, and antimicrobial peptides and mixing them at low temperature to form a sol mixture. After homogenization, degassing, and heating, a semi-solid gel is formed, and finally sterilization is performed to obtain the final product. This invention's gel combines *Pseudolarix amabilis* oil with hyaluronic acid, chitosan, and antimicrobial peptides, exerting anti-inflammatory, antibacterial, angiogenesis-promoting, and epithelial cell migration-promoting effects. It can significantly accelerate wound healing, inhibit scar hyperplasia, and exhibits excellent biocompatibility and no cytotoxicity.

This invention belongs to the field of biomedical materials and tissue engineering technology, and provides a decellularized matrix composite hydrogel dressing, its preparation method, and its application. The decellularized matrix composite hydrogel dressing provided by this invention includes a hydrogel matrix and ginger exosome-like nanoparticles (GELNs) dispersed in the hydrogel matrix; the hydrogel matrix is ​​prepared from raw materials including decellularized dermal matrix and decellularized aortic adventitia matrix. The composite hydrogel dressing of this invention has a three-dimensional porous structure, enabling sustained release of GELNs. It also significantly accelerates the healing process of full-thickness skin defects by downregulating the TLR4 / MyD88 / NF-κB signaling pathway, synergistically regulating macrophage polarization towards the M2 type, and simultaneously promoting angiogenesis and collagen deposition. The dressing has widely available raw materials, good biocompatibility, and broad application prospects in the field of skin wound repair.

The present disclosure provides human placenta-derived proangiogenic stem cells hPASCs and applications thereof. The human placenta-derived proangiogenic stem cells hPASCs of the present disclosure are preserved in the China Center for Type Culture Collection, Wuhan University, Wuhan, China, on April 2, 2025, with the preservation number CCTCC NO: C202592. The hPASCs of the present disclosure contain a subpopulation of angiogenic cells and have stem cell "stemness", and have angiogenic effects in vitro and in vivo, and can be used for the treatment of cardiovascular diseases and other diseases, and the vascularization of artificial organs and tissues.

Ensuring cell survival and promoting rapid vascularization after transplantation is an important for achieving successful tissue regeneration. Disclosed herein are methods designed to enhance cell survival and stimulate neovascularization and / or vascularized tissue regeneration after transplantation in a subject. The disclosed approach involves using a compound to pre-treat cells, stabilizing or activating HIF-1α under normal oxygen conditions, thereby increasing the therapeutic potential of the treated cells and triggering an angiogenic / vasculogenic response, particularly for dental pulp regeneration.

The application relates to a butyric acid-loaded outer vesicle, a preparation method, a medicine and application, which comprises endothelial cell outer vesicles and butyric acid loaded on the endothelial cell outer vesicles. The application can efficiently deliver butyric acid and specifically improve vascular endothelial cell aging, promote angiogenesis and tissue repair.

The application discloses a pancreatic cancer JAK2 and STAT3 dual-target inhibitor containing a phenoxy acetic hydrazine compound and application thereof, and the dual-target inhibitor comprises a compound containing a phenoxy acetic hydrazine structure. The dual-target inhibitor comprises a pharmaceutically acceptable salt. The pancreatic cancer JAK2 and STAT3 dual-target inhibitor containing the phenoxy acetic hydrazine compound is applied to the preparation of a drug for treating pancreatic cancer. The drug has a new use in treating pancreatic cancer by inhibiting tumor proliferation, migration and invasion, angiogenesis, epithelial mesenchymal transition, blocking a cell cycle and promoting cell apoptosis. The pharmaceutical preparation of the drug composition comprises tablets, capsules, syrup, suspensions and injections. A drug for treating pancreatic cancer comprises the pancreatic cancer JAK2 and STAT3 dual-target inhibitor containing the phenoxy acetic hydrazine compound and a pharmaceutically acceptable carrier. The application has the beneficial effect that the pancreatic cancer JAK2 and STAT3 have a better inhibiting effect, the experimental results show that the inhibiting effect is good, the application prospect is wider, and the safety is better.