104results about How to "Improve cell activity" patented technology

A ceramic anode structure obtainable by a process comprising the steps of: (a) providing a slurry by dispersing a powder of an electronically conductive phase and by adding a binder to the dispersion, in which said powder is selected from the group consisting of niobium-doped strontium titanate, vanadium-doped strontium titanate, tantalum-doped strontium titanate, and mixtures thereof, (b) sintering the slurry of step (a), (c) providing a precursor solution of ceria, said solution containing a solvent and a surfactant, (d) impregnating the resulting sintered structure of step (b) with the precursor solution of step (c), (e) subjecting the resulting structure of step (d) to calcination, and (f) conducting steps (d)-(e) at least once.

The invention discloses a natural plant whitening soap, which contains the following components: 9-13% of aloe glue, 25-40% of plant whitening liquid, 40-54% of grease substance, 5-10% of strong base substance, 2-3% of sodium chloride, 0.5-0.8% of fungicide, and 0.2-0.7% of germicide. The soap provided by the invention has functions of diminishing inflammation, sterilizing and protecting the skin, and also has remarkable effects of eliminating speckle and whitening the shin; and the soap has the capabilities of whitening, smoothening and refreshing the skin after used for a long time.

The invention relates to a porous bacterial cellulose skin repair material with a density structure and a preparation method thereof. The porous bacterial cellulose skin repair material with the 'upper dense and lower loose' structure similar to human skin is prepared by controlling the culture condition of bacterial cellulose and adding sustained-release microspheres in the culture process. A loose layer is tightly combined with a dense layer, so that obvious physical layering is avoided, and the structural continuity is good; and gradient changes of the structures are produced in the loose layer and the dense layer, and multiple pores distributed uniformly are formed in the loose layer, so that the upper dense and lower loose gradient structure of the human skin is simulated to the utmost degree. Cells easily enter the material, the healing period of a wound is obviously shortened, and proliferation of healed scars is effectively reduced; and good air permeability and water holding property of the bacterial cellulose are kept, the wound surface can be kept in a wet environment, and healing of the wound surface is facilitated. The forming process is simple, the culture period is short, the preparation process is environment-friendly, simple, convenient and quick, and the preparation cost is low.

The invention aims to provide an efficient culture method of CIK cells. The prepared CIK cell has the characteristics of high multiplication speed, large cell quantity, high cell viability, high killing capacity on cancer cells and the like. The efficient culture method mainly characterized in that a culture flask coated with laminin and fibronectin is used for culturing PBMCs (peripheral blood mononuclear cells), and the cells can be in a half-adherence state by means of the absorption effect of the two kinds of protein, accordingly, the cells can be stimulated by various factors, and the two kinds of protein can promote CIK cell multiplication; and with the adoption of an anti-CD28 monoclonal antibody, the co-stimulation signal of a T cell subset in the CIK cells can be stimulated, and the activation of the CIK cells is promoted. So far, a research report on jointly using laminin, fibronectin and the anti-CD28 monoclonal antibody to prepare the CIK cells is absent. The method for increasing the cell number and improving the killing activity by adding the three factors during preparation of the CIK cells is firstly provided.

The invention discloses a serum-free and DMSO-free cell cryopreservation solution, which comprises a 16.762-16.825 g/L DMEM/F-12 basal culture medium, 32-34 mg/L cytokines, 0.2-0.8 mg/L hydroxyethyl starch, 1-3 mg/L vitamins, 0.15-0.16 g/L antibiotics, 5-6 mg/L protein polypeptide, 1-4 mg/L lipids and a 0.1-0.41 g/L intracellular and extracellular cryoprotectant. According to the technical scheme,the obtained cell cryopreservation solution does not contain serum or DMSO components, the components of the formula are determined, the cell cryopreservation solution has high biological safety andclinical application prospects, and the cell cryopreservation liquid has a cell recovery survival rate of more than 85%, can maintain good cell activity and good physiological characteristics, and issuitable for the related research fields of primary cells and stem cells.

The present invention relates to therapeutic compositions for treating or preventing obesity and obesity-related disorders in a subject. The present invention also relates to the use of PGC-1 expression levels to determine the safe dosage range for known or putative respiration uncoupling agents for use as anti-obesity therapeutics. The present invention further relates to methods for identifying new compounds that have respiration uncoupling activity.

The invention discloses a nutritional buckwheat sprout cultivation method. The nutritional buckwheat sprout cultivation method includes the following steps of 1 germination accelerating, 2 sowing, 3 management and 4 harvesting. The nutritional buckwheat sprout cultivation method is simple in process, a cultivation cycle is shortened to be 8-9 days, the germination rate is 94-96%, the resistance is strong, and buckwheat sprouts vigorously grow, and are rich in nutrition, diversified in health care function and capable of promoting metabolism, enhancing immunity, protecting cardiac-cerebral blood vessels, maintaining beauty, keeping young, protecting the mouth, relieving internal heat or fever and resisting cancers. Buckwheat seeds are subjected to microwaves, ultrasound and soaking in sprouting liquid, the sprouting liquid rapidly penetrates into the seeds, the time is saved, nutrition of the seeds and cell activity are strengthened, the germination rate of the seeds is improved, resistance is enhanced, and the germination time is shortened. After germination accelerating, non-germinated seeds are directly added to a cultivation matrix of a seedling tray, antimicrobial and insecticidal effects are achieved, and the survival rate of the seeds is improved, so that buckwheat sprouts grow upright, are rich in nutrition, have a health-care function, adsorb harmful substances and are nutritional, safe, green and healthy.

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL/mL, human Source AB type serum 5‑15 μL/mL, human transferrin 5‑15 μg/mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.

The invention discloses an adipose derived stem cell (ADSC) large-scale culture method. The method selects a non-enzymatic digestion method (TrypLE) to collect adipose derived stem cells adhering to walls, adopts a minimum TrypLE dosage to maximumly reduce the damage of the cell digestion process to cells, and selects the optimum inoculation density of 1.3*10<4>/cm<2> to culture cells, uses a serum-free medium (UltraCULTURE MEDIUM), and adds appropriate concentration EGF into the medium so as to conduct large-scale culture in a phi 15cm culture dish. Thus, the passage number of ADSC is increased to over 15 and the original dryness can still be maintained, thereby greatly satisfying the requirement of mesenchymal stem cells for scientific research and application.

Device and method for treating hyperhidrosis, neuropathy, skin, circulation, muscle stimulation, by application of a fabric including an elemental zinc particle deposition to a treatment area of the skin.

The invention discloses an infant-nutrition bag preparation with the effects of benefiting the intelligence and reinforcing the brain. The infant-nutrition bag preparation comprises the following materials in parts by weight: 100-120 parts of while milk powder, 30-40 parts of soybean protein powder, 60-80 parts of whey protein powder, 12-20 parts of fructooligosaccharide, 5-10 parts of honey, 0.2-0.3 part of vitamin, 0.1-0.2 part of DHA, 0.1-0.2 part of ferric pyrophosphate, 0.2-0.4 part of zinc citrate, 0.25-0.5 part of calcium carbonate, 6-9 parts of probiotic powder, 15-25 parts of walnut powder and 6-12 parts of fruit powder. The infant-nutrition bag preparation disclosed by the invention has the advantages that the components are reasonable, the mouthfeel is good, the nutrition is rich, the absorption and the digestion are easy, the development of the physique of an infant is promoted, meanwhile the effects of benefiting the intelligence and reinforcing the brain are achieved, and the development of the intelligence of the infant is effectively promoted.

The invention relates to the field of cell culture, in particular to a gelatin microsphere and a preparation method thereof. The preparation method of the gelatin microsphere provided by the invention comprises the steps that gelatin is dissolved into water; different emulsifying agents are respectively added; stirring is performed to obtain emulsion; different dehydrating agents are added into the emulsion; cleaning, drying and sieving are performed; then, gelatin microsphere crude products are obtained; an ethanol solution containing Jinniping crosslinked curing agents is added into the gelatin microsphere crude products; the gelatin microsphere is obtained through preparation. Experimental results show that the gelatin microsphere prepared by the preparation method provided by the invention has smaller toxic action on cells; the toxicity on the cells can be reduced in the cell amplification culture process; the cell activity is improved and the degradation effect is better.

The invention discloses a double-layer microfluidic chip for tumor cell sorting, comprising a first chip and a second chip positioned below the first chip; the surface of the first chip has a sample passage, the surface of the first chip facing the sample passage has a buffer passage, and the sample passage is cross-communicated with the buffer passage. Compared with CellSearch detection method, the technique of using the double-layer microfluidic chip to analyze tumor cells can be finished continuously in one step with no need for multiple steps and is simple to perform, detection speed is high, the purity of enriched CTCs is higher, no antibody is used, and CTCs obtained by the technique have high cell activity and may be better used in subsequent detection and tumor specificity research.

The invention discloses whitening restoring cream which is prepared from the following components in percentage by weight: 0.1 to 1% of whitening restoring composition, 0.2 to 2.5% of an emulsifier, 2to 11% of an emollient, 3 to 17% of a humectant, 2 to 33% of a skin conditioner, 1 to 3.7% of a thickener, 0.01 to 0.05% of a chelating agent, 0.1 to 1.3% of a preservative and the balance of deionized water. The whitening restoring composition is prepared from the following raw materials in parts by weight: 0.1 to 5 parts of almond kernel, 0.1 to 5 parts of scutellaria root and 0.1 to 5 parts ofpeach fruits. After the whitening restoring composition is added into the whitening restoring cream, skin metabolism can be promoted, damaged skin can also be restored, and skin microcirculation is promoted; effective active ingredients in the whitening restoring composition are very excellent in penetrability, can provide nutrition for skin, enable the skin to be tender and rich in elasticity and enable the skin to be ruddy and bright white.

The invention discloses a hermetia illucens breeding method and a hermetia illucens puparium powder preparation method. The hermetia illucens puparium powder preparation method includes the steps of S1, drying hermetia illucens puparium and then grinding the same into powder; S2, adding powdered hermetia illucens puparium in a sodium hydroxide solution, stirring, separating and filtering; S3, adding the powdered hermetia illucens puparium in a hydrochloric acid solution, stirring, separating and filtering; S4, adding the powdered hermetia illucens puparium in another sodium hydroxide solution,stirring, separating and filtering; S5, drying the powdered hermetia illucens puparium, and then screening particles of pre-treated powder of hermetia illucens puparium. The invention further discloses a hermetia illucens puparium composite material and thin film preparation method, and an antibacterial and anti-mould additive. According to the invention, productivity of chitosan of hermetia illucens puparium can be increased effectively, prepared powdered hermetia illucens puparium can be used for preparation of hermetia illucens puparium high-polymer composite fiber, thin film and the like,and antibacterial effect is improved greatly. Further, a mixture of the powdered hermetia illucens puparium and oyster shell powder can be used as the novel antibacterial anti-mould additive.

The invention relates to a skincare composition as well as use and method of the same for preventing and delaying skin aging. The skincare composition comprises resveratrol and tea polyphenol, wherein the ratio of resveratrol to tea polyphenol is 1-20 to 1-20. In the invention, from overall and balanced perspectives, skin aging mechanism is studied at many angles and many levels; and a plurality of safe and efficient anti-aging active substances are blended to form the composition in order to improve cell activities, clean free radicals, rebuild skin structure, and regulate metabolism of a body based on endogenous reasons for the aging and pathological changes caused by the aging, so that the skincare composition is more obvious and safer to achieve the effect of delaying the skin aging.

The invention belongs to the technical field of salix integra cultivation, and particularly relates to a fertilizer applying method for improving salix integra toughness. The method includes the steps: applying 1100-1200kg of microorganism organic fertilizers into soil of planting land per mu before cutting of salix integra; spraying nutrient solution 8-10 days after cuttage and sprouting of the salix integra, spraying the nutrient solution once every 18-20 days, and continuously spraying the nutrient solution for three times. The microorganism organic fertilizers are applied before cuttage, nutrition needed for plant growth is provided, growth of salix integra root systems is facilitated, ability of the salix integra root systems absorbing nutrients is improved, salix integra bodies are smooth, diseases and insect pests are effectively prevented and treated, soil acidification and soil hardening are effectively restrained, continuous updating ability of the salix integra is enhanced, the nutrient solution is continuously sprayed after sprouting, cell activity of salix integra branches is effectively enhanced, branching and branch growing ability of the salix integra is enhanced, cellulose synthesis is facilitated, the toughness of the salix integra is effectively improved, and toughness keeping capacity is effectively improved.

The invention belongs to the field of fertilizers for crops and particularly relates to an ecological functional compound fertilizer and a preparation method thereof. The ecological functional compound fertilizer is prepared from the following main components: sludge, a compound fertilizer, a microbial agent, an organic waste, a soil heating agent and an oxygen supplier. The sludge obtained from the treatment of municipal domestic wastewater and the organic waste serve as organic matters, contain multiple nutrients and can be combined with the compound fertilizer to increase the utilization rate of the compound fertilizer and increase the content of organic matters in soil; the added soil heating agent can preserve the temperature around the roots of crops in deep soil and prevent crops from being frostbitten and frozen to death; the added oxygen supplier can supply oxygen to microorganisms in soil and cells in crop roots to increase the activity of the microorganisms in soil and the crop roots, accelerate the absorption of the ecological compound fertilizer, enhance the adversity, disease and cold resistance of crops, improve the structure and fertility of soil and promote the growth of crops; the pollution of sludge to environment can be reduced, and the sludge can be reutilized; and the ecological compound fertilizer is safe to people, animals and environment and is natural, innoxious and environmentally friendly.

The invention relates to an in-vitro three-dimensional amplification culture method for NK cells. The culture method comprises the following steps: obtaining NK cells; inoculating a three-dimensional culture bracket with the NK cells; and performing in-vitro three-dimensional amplification culture, wherein the three-dimensional culture bracket is a chitosan-sodium alginate bracket. Through the porous structure as well as the mechanical properties and biocompatibility of the chitosan-sodium alginate bracket, the growing space of the NK cells and the contact area with nutrient substances are enlarged, and the NK cells can be uniformly distributed in the chitosan-sodium alginate bracket, thus the cell activity of the NK cells can be improved, and the improvement of the amplification rate and killing activity of the NK cells is facilitated; and the defects such as low activity and low amplification rate of NK cells caused by culturing the NK cells with feeder cells in a two-dimensional environment in the prior art are solved.

The invention discloses an amidophenyl-1,3,4-oxadiazole compound shown in the formula (I). The invention also discloses a preparation method of the amidophenyl-1,3,4-oxadiazole compound. The preparation method comprises that an amino-compound II and a substituted aliphatic acid III are condensed to form the amidophenyl-1,3,4-oxadiazole compound. The invention also discloses a use of the amidophenyl-1,3,4-oxadiazole compound I in preparation of a drug for treating type II diabetes. The amidophenyl-1,3,4-oxadiazole compound has a non-AMP structure type, can reduce side-effect risk, can obviously inhibit FBPase in a molecule level, can substantially inhibit glucose generation in a cell level, and has good cell viability and good druggability.

The invention provides anti-allergy composition. The anti-allergy composition is prepared from a traditional Chinese medicine mixture, an anti-inflammatory agent, a humectant, a preservative and an antioxidant, wherein the anti-inflammatory agent can effectively inhibit secretion of inflammatory factors and resist inflammation effectively, and the allergy resisting purpose is achieved; the antioxidant can protect lipid of a cell membrane from overoxidation, relieve inflammatory response and indirectly play an allergy resisting role; the traditional Chinese medicine mixture can improve cell viability and strengthen skin health. The anti-allergy composition can effectively help the skin resist invasion of sensitive substances, improve cell viability and have the anti-inflammatory and anti-allergy effects, and after the composition is applied to cosmetics, the cosmetics can be endowed with good moisturizing, whitening and allergy resisting effects.

The invention discloses a method for preparing DEHP degrading bacterial agent and a method for effectively reducing DEHP pollution in vegetable production. The bacterial agent is prepared by the steps: activating rhodococcus sp. 2G and then carrying out fermented culture, centrifuging the fermentation liquid, collecting bacteria, adding a protective agent to the bacteria, pre-freezing, and carrying out vacuum freeze-drying of the pre-frozen bacteria to obtain the DEHP degrading bacteria freeze-dried bacterial agent. A certain proportion of the bacterial agent is added in a farmland having soilpolluted by the DEHP, so the accumulation amount of the DEHP in planted vegetables is reduced while the DEHP in the soil is removed; by addition of the DEHP degrading bacterial agent and colonizationof the degrading bacteria at vegetable rhizospheres, the residual amount of the DEHP in the polluted soil and the planted vegetables can be effectively reduced. The method not only remediates the DEHP polluted soil in farmland, but also produces agricultural products safely, namely a concept of remediating while producing, has the advantages of high treatment efficiency, low cost and no secondarypollution, and has important application prospects in prevention and control of organic pollution in the soil of the farmland.

The invention provides a stem cell preparation for treating aplastic anemia and a preparation method thereof. The defects in the prior art are overcome, the occurrence rate of the graft versus host disease is decreased, more mesenchyme stem cells with the high immunosuppression function are obtained within a short period of time, the clinical requirements are met, a new thought is provided for the transplantation effect of stem cells, and important practical application value is brought into play. The invention further provides the preparation method of the stem cell preparation for treating aplastic anemia. According to the method, the mesenchymal stem cells are induced and cultivated through a special method, the immunosuppression capacity of the mesenchymal stem cells can be effectively improved and enhanced, and the mesenchymal stem cells can be further prepared into the stem cell preparation for treating aplastic anemia.

The invention discloses a culture method of human colorectal cancer tissue organoid, which comprises the following steps: washing human colorectal cancer tissues and putting them into a transfer culture medium, cleaning and shearing; further cleaning, centrifuging, carrying out enzyme digestion, filtering, and collecting cell clusters; and mixing the cell clusters with hydrogel, adding DMEM culture medium to culture after solidification to obtain the human colorectal cancer tissue organoids. According to the invention, gentamicin, amphotericin B, primocin and other antibiotics are added into the transfer culture medium, the digestion solution and the culture medium, so that microbial contamination can be reduced, and the method for obtaining the human colorectal cancer organoids through in-vitro culture is simple, convenient, rapid, high in success rate and good in organoid growth state. The invention further discloses application of the human colorectal cancer organoid in drug testing, and the co-culture model of human colorectal cancer tissue organoids and tumor-associated fibroblasts can be used as a patient individualized drug testing platform.

The invention discloses immune cell preserving fluid. The immune cell preserving fluid comprises 0-50 U/mL of recombinant human interleukin-2, 0-10 mg/mL of human albumin, 0-1.5 mg/mL of alpha-1,4-glucan, 0.5-2.0 mg/mL of glucose, 3.5-5.0 mg/mL of sodium citrate, 2.0-3.5 mg/mL of sodium gluconate, 0.2-0.5 mg/mL of potassium chloride and 0.2-0.5 mg/mL of magnesium chloride. The immune cell preserving fluid has the advantages of being suitable for cell preservation within a wide temperature range, and capable of guaranteeing high cell viability of immune cells and facilitating follow-up cell experiment or clinical treatment.

The invention discloses a method for regulating osmotic pressure stress of Pseudomonas glabrata, belonging to the field of bioengineering. The invention regulates the osmotic stress resistance of Pseudomonas glabrata by overexpressing or deleting the coding gene CgRDS2 of the native transcription factor. The results showed that the biomass, cell viability and membrane integrity of Pseudomonas glabrata decreased by 23%, 46% and 47.6%, respectively, under osmotic stress compared with wild-type strain after the deletion of CgRDS2 gene. After overexpression of CgRDS2 gene, the biomass increased by10%, cell viability increased by 39% and cell membrane integrity increased by 12.1% under 1.5 M NaCl stress.

The invention discloses an ophiopogon polysaccharide and white atractylodes rhizome polysaccharide composition and preparation method thereof, and discloses application of the ophiopogon polysaccharide and white atractylodes rhizome polysaccharide composition in promotion of proliferation of human umbilical cord mesenchymal stem cells. The drug composition disclosed by the invention can enable mesenchymal stem cells to keep relatively high activity, substantially increase the proliferation speed and prevent differentiation of the cells. Application of the umbilical cord mesenchymal stem cellsprepared through the invention has important significance for a scientific research and clinical application.

The invention belongs to the technical field of tissue culture and discloses a method for ultralow-temperature preservation of anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus. By proper pretreatment and adoption of a mixed cryoprotectant, the method is suitable for freeze preservation of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus, cell viability of theembryonic callus unfrozen at 30-35 DEG C reaches 100% maximally, and the embryonic callus subjected to ultralow-temperature preservation has no evident difference from embryonic callus subjected to normal proliferation in appearance and microstructure and still has a function of differentiation for forming somatic embryos. An ultralow-temperature preservation system of the anti-bursaphelenchus-xylophilus pinus massoniana embryonic callus is established preliminarily, and technical references are provided for long-time embryonic maintaining of the resistant pinus massoniana embryonic callus.

The invention discloses a feed capable of reducing the attack rate of broiler chickens and a preparation method of the feed. The feed comprises the following raw materials of corn flour, soybean flour, selenium-enriched coarse rice powder, corn straw powder, sunflower seed meal, rice chaff, fruit peel powder, animal bone meal, hay meal, silkworm chrysalis meal, dried marine algae powder, green teapowder, a compound enzyme preparation, probiotics, a sweetening agent, a Chinese herbal medicine additive, table salt and a coating agent. According to the feed capable of reducing the attack rate ofthe broiler chickens and the preparation method of the feed provided by the invention, the formula and the preparation technology of the feed are optimized, so that the obtained feed is reasonable innutrition collocation, good in palatability, good in stability and long in quality guarantee period, nutrient loss is not liable to cause in the transportation process, the constitutions of the broiler chickens are effectively improved, the attack rate and the mortality rate of the broiler chickens are significantly reduced, and the breeding economic benefits of the broiler chickens are increased.

The invention relates to an in-vitro expansion culture method of NK cells. The culture method comprises the following steps that the NK cells are acquired; the NK cells are subjected to in-vitro expansion culture in a culture medium containing an insulin-like growth factor-1. The insulin-like growth factor-1 replaces feeder cells for carrying out expansion culture on the NK cells, so that the steps of NK cell expansion culture are simplified; the cell viability, the multiplication rate and the killing activity of the NK cells can be improved through the insulin-like growth factor-1, the insulin-like growth factor-1 can stimulate the NK cells to secrete the endogenic insulin-like growth factor-1 to further improve the killing activity of the NK cells, and therefore the defects that in the prior art, due to the fact that the feeder cells are used for carrying out multiplication culture on the NK cells, the NK cell multiplication rate is low, and the killing activity is poor are overcome.