12results about How to "Improve cell activity" patented technology

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention discloses a preparation method and application of a prostate cancer tissue single-cell suspension. The preparation method of the prostate cancer tissue single-cell suspension comprises the following steps: S1, tissue pretreatment: putting a prostate cancer tissue sample into a low-temperature pretreatment buffer solution, and cutting the prostate cancer tissue sample into tissue fragments; s2, circular enzymolysis: mixing the tissue fragments with a composite enzymatic hydrolysate, and performing digestion for multiple rounds under an oscillation condition; after each round of digestion, collecting supernate into a stop solution containing serum, repeatedly digesting the remaining tissues for multiple times, and combining the supernate to obtain a cell crude suspension; wherein the composite enzymatic hydrolysate contains collagenase, protease, nuclease, an androgen receptor stimulant and / or a ROCK inhibitor; and S3, sequentially carrying out separation and purification on the cell crude suspension through filtration and density gradient centrifugation to obtain the prostate cancer tissue single-cell suspension.

The invention discloses a skin-derived staphylococcus warneri CCSM004 prepared metagen with a skin barrier repairing effect, and belongs to the technical field of microorganisms and the technical field of medicines. The staphylococcus warneri CCSM004 provided by the invention can generate L-glycine-valine dipeptide and oleamide, and a fermentation supernatant prepared from the staphylococcus warneri CCSM004 has a good effect of repairing a barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, IVL, AQP3, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri has a huge application prospect in preparation of external cosmetics or medicines for repairing the skin barrier.

The utility model discloses a tea perfuming device for tea powder preparation, which comprises a base, the outer wall of the top of the base is fixedly connected with a driving seat, the inner wall of the driving seat is slidably connected with a stirring barrel, the tea perfuming device further comprises a spraying device, and the outer wall of the bottom of the spraying device is fixedly connected with the outer wall of the top of the driving seat; the utility model relates to the technical field of tea perfuming, according to the spraying device, through the cooperation of a spraying head and a spraying pipe, a water pump rapidly pumps perfume liquid in a spraying box, the perfume liquid is timely conveyed to the spraying head through a water outlet pipe and the spraying pipe and pushes a rotating paddle to rotate, and the rotating paddle drives the whole spraying head to slide and rotate along an annular groove through a rotating shaft; the atomized spice liquid is uniformly sprayed on the tea leaves through the atomizing nozzle, the stirring barrel can be heated through the heating plate arranged on the inner wall of the driving seat, and the tea leaves can better absorb the fragrance of the spice liquid by properly increasing the temperature.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to a cyclic peptide toxin Amanitin derivative, its preparation method, and its application. Based on the natural Amanitin compound, glycine was replaced with alanine; simultaneously, the eighth amino acid was also replaced. The synthesized cyclic heptapeptide was sequentially oxidized, and proline, phenylalanine, dibutylisobutyric acid, and dihydroxyisoleucine were linked to design and synthesize a series of Amanitin derivatives, namely, products 1 to compounds 12 of this invention, which are 12 cyclic peptide toxin derivatives, and they exhibit good cellular activity.

This invention relates to a myocardial repair patch based on multi-material printing, its preparation, and its application, belonging to the field of bio-3D printing and regenerative medicine technology. The invention uses PLCL and GelMA hydrogel loaded with active cardiomyocytes as bio-inks, and introduces gelatin gel as a heat buffer material. An orthogonal network structure is constructed using multi-material bio-3D printing technology to prepare a myocardial repair patch with excellent dynamic adaptability to the myocardial interface and good biocompatibility. The myocardial injury repair patch prepared by this invention achieves a structure-function integrated design through the synergistic effect of the three materials, significantly overcoming the limitations of single materials in terms of mechanical properties, biological function, or thermal compatibility. The mechanical properties of the myocardial injury repair patch prepared by this invention can precisely match those of natural myocardium, while possessing high cell activity, effectively inhibiting fibrosis, improving myocardial function, and achieving true active repair. Furthermore, the process conditions are controllable, demonstrating good translational potential.

The invention discloses staphylococcus warneri CCSM003 separated from human skin and capable of repairing a skin barrier and a metagen of the staphylococcus warneri CCSM003, and belongs to the field of microbial technologies and medicines. The staphylococcus warneri CCSM003 provided by the invention can be fermented to generate the L-glycine-valine dipeptide, and the staphylococcus warneri CCSM003 has a good effect of repairing a barrier function in the aspect of external use and an application prospect, specifically, the activity of damaged HaCaT cells is improved in vitro; the gene level expression of FLG, IVL, AQP3, ZO-1, CLDN and Occludin can be improved at the same time in the aspect of improving functional protein and structural protein after damage. Therefore, the staphylococcus warneri has a huge application prospect in preparation of external cosmetics or medicines for repairing the skin barrier.

The invention discloses a gamma-PGA synthetase gene cluster pgsBCA '. The gamma-PGA synthetase gene cluster pgsBCA' is formed by sequentially connecting genes pgsB, pgsC and pgsA 'in series, the genes pgsB and pgsC are derived from a gamma-PGA synthetase gene cluster BA-pgsBCA of the bacillus altitudinis; the gene pgsA'is derived from a gamma-PGA synthetase gene cluster BM-pgsB 'C' A 'of bacillus megatherium. Bacillus amyloliquefaciens CF of which a genome knockout polyglutamate synthetase gene cluster is used as a host, and a recombinant polyglutamate synthetase gene cluster pgsBCA'is subjected to free expression through plasmids, so that a gene engineering strain capable of synthesizing gamma-L-PGA is obtained. When the strain is used for producing gamma-L-PGA in a 5L fermentation tank through amplification fermentation, the maximum yield can reach 10.5 g / L. Besides, the gamma-poly-L-glutamic acid is free of cytotoxicity and low in immunogenicity, so that the gamma-poly-L-glutamic acid has a wide application prospect in the fields of preparation of wound dressings, in-vivo absorption materials and the like. The invention provides a new material basis and technical support for application and development of polyglutamic acid in the fields of medicine and related biomedicine.

The invention provides a composition for preventing bone loss caused by cold exposure as well as a preparation method and application of the composition, and belongs to the technical field of pharmaceutical compositions. The composition provided by the invention is prepared from the following components in parts by weight: 1.18 to 1.58 parts of radix angelicae sinensis extract, 1.18 to 1.58 parts of flos carthami extract, 1.18 to 1.58 parts of semen persicae extract and 2.56 to 2.96 parts of fructus crataegi extract. The preparation method of the composition comprises the following steps: weighing the polymer carrier, adding the polymer carrier into precooled purified water, and adding ethanol; weighing a raw material mixture, and adding the raw material mixture into the polymer solution; performing spray drying to obtain fine powder, and performing vacuum drying to obtain the composition for preventing bone loss caused by cold exposure. The composition for preventing bone loss caused by cold exposure, prepared by the invention, has high solubility and high dissolution rate, and active ingredients take effect quickly; the bone loss caused by cold exposure can be prevented.

The application provides a serum-free cryopreservation solution and application thereof in cell cryopreservation. The serum-free cryopreservation solution comprises a first additive, a saccharide, an inorganic salt, a protein and a second additive; in the serum-free cryopreservation solution, the mass concentration of the first additive is 0.2-15% (w / v), the concentration of the saccharide is 2-15 mg / mL, the mass concentration of the inorganic salt is 0.7-1.2% (w / v), the concentration of the protein is 0.05-0.5 g / mL, and the concentration of the second additive is 0.5-5 mg / mL. The serum-free cryopreservation solution provided by the application ensures that the cell still has high survival rate, high activity and functional integrity after cryopreservation through the combined action of the above components, the composition of the cryopreservation solution is clear, and no animal-derived serum needs to be added, so that the immunogenicity risk, pathogen contamination and batch difference caused by the animal-derived serum are avoided, and the safety of the cryopreservation solution is significantly improved.

The invention discloses a culture method of DC-CTL cells, and particularly relates to the technical field of biotechnology and medicine. The invention provides a novel DC-CTL cell culture method based on iPSC, aiming at the technical problems that a traditional DC-CTL cell culture method is limited in cell source, greatly influenced by individual difference, poor in stability and repeatability, insufficient in cell activity and killing ability and the like. A self-made additive and a specific cell factor are combined, SOCS1-siRNA is transfected through a liposome transfection method, the SOCS1 gene is effectively silenced, and the T cell activity is enhanced. According to the method, the proportion of CD3 + CD8 + T cells in the DC-CTL cells reaches 85% or above, the content of IFN-gamma is larger than or equal to 500 pg / mL, the content of TNF-alpha is larger than or equal to 300 pg / mL, and the killing rates under the effect target ratios of 10: 1, 20: 1 and 40: 1 reach 57%, 63% and 72% respectively.