34results about How to "Improve cell activity" patented technology

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention belongs to the technical field of bioengineering, and provides a recombinant XVII type collagen as well as a preparation method and application thereof. A target gene sequence is obtained through bioinformatics predictive analysis and integrated design on the basis of a human XVII type collagen sequence, codon optimization is carried out, a T7 promoter in a plasmid vector is optimized, an engineering strain of the high-hydrophobicity humanized XVII type collagen is prepared, and the high-hydrophobicity humanized XVII type collagen is obtained. The strain is subjected to culture and inducible expression to obtain high-yield recombinant XVII type collagen. The recombinant XVII type collagen disclosed by the invention is reasonable in sequence design, large in expression quantity, simple in preparation method and stable in heredity, and rapid separation of the collagen can be realized in a production and preparation process by utilizing high hydrophobicity.

The invention provides a single-cell suspension and a preparation method thereof. Specifically, the preparation method comprises the following steps: 1) mixing a condensed-state cell sample to be dispersed with a pretreatment solution, and carrying out ultrasonic treatment; 2) mixing the sample obtained in the step 1) with the enzymatic hydrolysate, and carrying out an enzymatic hydrolysis reaction; 3) adding a nanometer material with the surface modified with a target cell specific marker, controlling the distribution of the nanometer material through an external magnetic field, and enriching target single cells; 4) separating the sample obtained in the step 3) to obtain a target single-cell suspension; wherein the conditions of the ultrasonic treatment and the enzymolysis reaction are controlled by an intelligent control system. According to the technical scheme, ultrasonic treatment and enzymolysis reaction are combined, an intelligent control system is used for controlling ultrasonic conditions and enzymolysis conditions respectively, and a nano material labeling technology is combined, so that the finally obtained cell suspension is high in cell viability and high in purity.

The utility model discloses a platelet shaking device for blood transfusion, which relates to the technical field of shaking devices and comprises a shaking box and a box cover. The device comprises a shaking box, a box cover is arranged on the upper side of the shaking box, a placement assembly is arranged in the middle of the interior of the shaking box and comprises an objective table, an electronic scale and a heat preservation plate, the objective table is arranged in the middle of the interior of the shaking box, a cavity is formed in the objective table, the electronic scale is fixedly arranged at the bottommost part of the cavity, and the heat preservation plate is fixedly arranged on the upper side of the electronic scale. A shaking mechanism is arranged on the surface of the rear side of the objective table, and a driving mechanism is arranged on the rear side of the shaking mechanism, so that the blood transfusion bag can be shaken, the cell viability of platelets in blood is further improved, the platelets in the blood are prevented from being accumulated together, and then the body of a patient is prevented from absorbing the platelets in the conveyed blood; the stability of the device can be guaranteed, blood transfusion efficiency can be improved, and practicability is high.

The invention relates to the field of biological medicine, in particular to a water-soluble alginate microcarrier as well as a preparation method and application thereof. The alginate microcarrier comprises a carrier core structure and a shell surface modifier, the micro-carrier core layer is formed by carrying out plasma crosslinking on water-soluble alginate, ethylenediamine tetraacetic acid salt, nitrilotriacetic acid salt, diethylene triamine pentaacetic acid and water-soluble divalent metal salt, and light-sensitive alginate and a photoinitiator can also be added, so that the micro-carrier core layer has two crosslinking networks; the shell layer is composed of one of gelatin, collagen, fibrinogen, albumin and fibrin, or the gelatin, the collagen, the fibrinogen, the albumin and the fibrin and other biocompatible polymers; the obtained microcarrier has the characteristics of high transparency and rapid degradation, and adherent cells can be amplified in vitro; the microcarrier obtained in the invention can be used for mesenchymal stem cell culture, and can be rapidly degraded under a mild cell condition to harvest cells.

The present invention relates to the field of medicines, and discloses a cyclic dinucleotide prodrug molecule and a preparation method and application thereof. The cyclic dinucleotide prodrug molecule has a structure as shown in Formula I, Formula II or Formula III, can autonomously cross the cell membrane, release the cyclic dinucleotide, and has high cell activity.

The invention discloses lactobacillus reuteri CCFM1403 with a skin repairing effect and a metagen of the lactobacillus reuteri CCFM1403, and belongs to the technical field of microorganisms and medicines. The lactobacillus reuteri CCFM1403 provided by the invention has the effect of improving the barrier function of the skin in two aspects of external use and oral administration, and is specifically shown in that when the thallus lysate of the lactobacillus reuteri CCFM1403 is externally used, the cell viability and the related barrier function of keratinocytes after injury and the expression of a structural protein gene level can be improved; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of TEWL in apparent aspect, water content of cuticle, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the lactobacillus reuteri CCFM1403. Therefore, the lactobacillus reuteri has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention discloses a metagen which is prepared from bifidobacterium adolescentis CCFM1404 and has the effect of improving skin health, and belongs to the technical field of microorganisms and medicines. The metagen (especially the thallus lysate) of the bifidobacterium adolescentis CCFM1404, provided by the invention, has a good skin barrier repairing effect, which is specifically shown in that the cell viability of keratinocytes after injury and the expression of FLG, IVL and LOR gene levels can be improved by externally applying the thallus lysate of the bifidobacterium adolescentis CCFM1404; the DNFB-induced mouse skin barrier injury can be relieved in the aspects of apparent TEWL and epidermis thickness, up-regulation of epidermis and basal layer protein and the like by oral administration of metabiotics (including thallus lysate and fermentation supernatant) of bifidobacterium adolescentis. Therefore, the bifidobacterium adolescentis has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention discloses a multi-enzyme preparation for industrial cell dissociation as well as a use method and application thereof, and belongs to the technical field of extraction of seed cells for industrial culture of meat. According to the invention, a multi-enzyme preparation composed of a component 1 and a component 2 is adopted; the component 1 comprises neutral protease, hyaluronidase and elastase; and the component 2 comprises matrix metalloproteinase, papain and plasmin. The invention also discloses a preparation method and application of the multienzyme preparation. The reasonable enzyme ratio and the optimized treatment conditions are beneficial to protecting the integrity of cell membranes, maintaining high cell activity and remarkably improving the dissociation efficiency and the cell recovery amount; the complex enzyme preparation is high in adaptability, can be used for various tissue types and dissociation requirements, and is convenient for standardized and large-scale production; in combination with automatic dissociation equipment, the compound enzyme preparation can realize efficient and stable industrial operation, and provides important technical support and wide application prospects for the fields of meat culture industry, tissue engineering, cell therapy and the like.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention belongs to the technical field of biological medicines, and particularly relates to exosome loaded resveratrol as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out pretreatment by using sodium carboxymethyl cellulose, and loading resveratrol into the exosome in combination with ultrasonic and co-incubation. The preparation method provided by the invention is simple to operate, high in loading efficiency and suitable for large-scale production. The exosome provided by the invention is loaded with the resveratrol, so that the bioavailability of the resveratrol is enhanced, and the exosome has remarkable effects of removing ROS (Reactive Oxygen Species) and inhibiting melanin secretion of cells.

The invention discloses a preparation method and application of a prostate cancer tissue single-cell suspension. The preparation method of the prostate cancer tissue single-cell suspension comprises the following steps: S1, tissue pretreatment: putting a prostate cancer tissue sample into a low-temperature pretreatment buffer solution, and cutting the prostate cancer tissue sample into tissue fragments; s2, circular enzymolysis: mixing the tissue fragments with a composite enzymatic hydrolysate, and performing digestion for multiple rounds under an oscillation condition; after each round of digestion, collecting supernate into a stop solution containing serum, repeatedly digesting the remaining tissues for multiple times, and combining the supernate to obtain a cell crude suspension; wherein the composite enzymatic hydrolysate contains collagenase, protease, nuclease, an androgen receptor stimulant and / or a ROCK inhibitor; and S3, sequentially carrying out separation and purification on the cell crude suspension through filtration and density gradient centrifugation to obtain the prostate cancer tissue single-cell suspension.

The present invention relates to a composition containing Antrodia cinnamomea powder and a preparation method and application thereof, belonging to the technical field of medicines, the composition comprises Antrodia cinnamomea extract, Hovenia dulcis extract, Pueraria root extract, Psoralea corylifolia extract, Cyperus rotundus extract and cotton sugar; Antrodia cinnamomea extract contains triterpenoid compounds; Pueraria root extract is an extract below 10kDa obtained by graded enzymolysis of Pueraria root with hemicellulase and trypsin; Psoralea corylifolia extract is an extract below 5kDa obtained by enzymolysis of Psoralea corylifolia protein with alkaline protease; Cyperus rotundus extract is an extract below 5kDa obtained by enzymolysis of Cyperus rotundus with subtilisin and trypsin. Pueraria root extract, Psoralea corylifolia extract and Cyperus rotundus extract all have the effect of promoting hangover, and have a significant synergistic effect when used in combination with Antrodia cinnamomea extract, can promote cell and enzyme activity, promote the decomposition of alcohol in the body, quickly improve the drunken state, and do not cause vomiting.

The present invention discloses a method for utilizing 89 A method for Zr imaging tracing of human mesenchymal stem cells, the method is performed as follows: 89 HBSS was added to the Zr-DFO-NCS reaction solution and the pH was adjusted to obtain 89 Zr-DFO-NCS mixed solution; collect human mesenchymal stem cells and use 89 Resuspend the cells in Zr-DFO-NCS mixed solution and incubate; remove the supernatant by centrifugation, then wash the labeled cells several times with PBS, re-plate and continue to culture. 89 Zr-labeled human mesenchymal stem cells. The present invention uses DFO as an intermediate chelating agent to improve 89 The stability of Zr and hMSCs labeling and the accuracy of in vivo tracing results. 89 Zr-hMSCs rather than simply washing, firstly restore cell activity, and secondly remove the markers on cell debris. 89 Zr improves the accuracy of imaging tracing results.

The invention belongs to the technical field of biomedical engineering, and provides a preparation method of cell sphere modularized bio-ink and application of the cell sphere modularized bio-ink in the field of myocardial repair treatment. Firstly, stem cell spheres are prepared on the basis of a microarray, a protein / polyphenol composite coating is introduced to the stem cell spheres, the stem cell spheres serve as a multifunctional armor, immune protection and immune regulation treatment aiming at a host immune system are provided, the hostile microenvironment is reversed, and the healing process of infarction myocardial tissue is promoted. Secondly, a biological material with good biocompatibility is selected to prepare gel microspheres carrying bioactive substances by adopting a micro-flow liquid drop control technology, the gel microspheres and the coated cell spheres are further closely packed to form cell sphere particle gel, disease treatment is performed in a direct injection or biological 3D printing mode, and good application prospects are achieved.

The invention provides a mesenchymal stem cell drug prescription and a preparation process thereof, and belongs to the technical field of biological medicines. The mesenchymal stem cell drug prescription combination provided by the invention comprises a mesenchymal stem cell solution and a self-assembly solution, the mesenchymal stem cell solution is composed of mesenchymal stem cells, human serum albumin, heparin, dextran 40 and a compound electrolyte solution, and the self-assembly solution is composed of protamine, dipotassium glycyrrhizinate, rosmarinic acid and normal saline. The mesenchymal stem cell drug prescription combination provided by the invention can improve the cell viability and stability of the mesenchymal stem cells, highly express CD73, CD90, CD105, HGF and sTNFR1, has a better immune regulation function, and provides a new thought for clinical application of the mesenchymal stem cells.

The invention discloses a skin-derived staphylococcus warneri CCSM004 prepared metagen with a skin barrier repairing effect, and belongs to the technical field of microorganisms and the technical field of medicines. The staphylococcus warneri CCSM004 provided by the invention can generate L-glycine-valine dipeptide and oleamide, and a fermentation supernatant prepared from the staphylococcus warneri CCSM004 has a good effect of repairing a barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, IVL, AQP3, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri has a huge application prospect in preparation of external cosmetics or medicines for repairing the skin barrier.

The invention relates to an anti-wrinkle composition and application thereof. The anti-wrinkle composition comprises a nostoc sphaeroids kutz extract, hydroxydecyl ubiquinone, rutin and raspberry ketone glucoside. The invention creatively finds that the common nostoc extract, the hydroxydecyl ubiquinone, the rutin and the raspberry ketone glucoside are matched for use and have a synergistic effect, so that the composition has good wrinkle-removing and anti-oxidation effects, and can recover skin elasticity, accelerate skin cell viability, reduce skin laxity and recover the health state of the skin.

The utility model discloses a tea perfuming device for tea powder preparation, which comprises a base, the outer wall of the top of the base is fixedly connected with a driving seat, the inner wall of the driving seat is slidably connected with a stirring barrel, the tea perfuming device further comprises a spraying device, and the outer wall of the bottom of the spraying device is fixedly connected with the outer wall of the top of the driving seat; the utility model relates to the technical field of tea perfuming, according to the spraying device, through the cooperation of a spraying head and a spraying pipe, a water pump rapidly pumps perfume liquid in a spraying box, the perfume liquid is timely conveyed to the spraying head through a water outlet pipe and the spraying pipe and pushes a rotating paddle to rotate, and the rotating paddle drives the whole spraying head to slide and rotate along an annular groove through a rotating shaft; the atomized spice liquid is uniformly sprayed on the tea leaves through the atomizing nozzle, the stirring barrel can be heated through the heating plate arranged on the inner wall of the driving seat, and the tea leaves can better absorb the fragrance of the spice liquid by properly increasing the temperature.

The invention discloses a cyclic peptide and application thereof, and particularly provides a cyclic peptide as shown in a formula I. The cyclic peptide provided by the invention has relatively good biological stability and good cell activity, and is suitable for clinical popularization and application. # imgabs0 #

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to a cyclic peptide toxin Amanitin derivative, its preparation method, and its application. Based on the natural Amanitin compound, glycine was replaced with alanine; simultaneously, the eighth amino acid was also replaced. The synthesized cyclic heptapeptide was sequentially oxidized, and proline, phenylalanine, dibutylisobutyric acid, and dihydroxyisoleucine were linked to design and synthesize a series of Amanitin derivatives, namely, products 1 to compounds 12 of this invention, which are 12 cyclic peptide toxin derivatives, and they exhibit good cellular activity.

The invention discloses a pharmaceutical composition containing high-activity umbilical cord MSC (mesenchymal stem cells). On the basis of HA-MSC, by reasonably compounding components such as cell growth factors, estrogen and nicotinamide, the disadvantages of high price, large side effect and the like of hormone products are effectively overcome, and the purpose of effectively preventing and / or treating ovarian aging is achieved.

The invention discloses a production method of BVDV and IBRV genetic engineering subunit fusion protein, which comprises the following steps: inoculating CHO seed cells of fusion expression BVDV-IBRV protein to a basic culture medium, carrying out feed supplement culture, inoculating on the 0th day, supplementing 3% of feed every day from the 1st day to the 6th day, then supplementing 3% of feed every other day, and finishing the feed supplement on the 12th day. Due to the fact that CHO cell genes are prone to denaturation, in order to express different foreign proteins, different culture conditions, different culture media, different material supplementing modes and the like can influence the density, activity and metabolism of CHO cells, the final target yield and the like.

This invention relates to a myocardial repair patch based on multi-material printing, its preparation, and its application, belonging to the field of bio-3D printing and regenerative medicine technology. The invention uses PLCL and GelMA hydrogel loaded with active cardiomyocytes as bio-inks, and introduces gelatin gel as a heat buffer material. An orthogonal network structure is constructed using multi-material bio-3D printing technology to prepare a myocardial repair patch with excellent dynamic adaptability to the myocardial interface and good biocompatibility. The myocardial injury repair patch prepared by this invention achieves a structure-function integrated design through the synergistic effect of the three materials, significantly overcoming the limitations of single materials in terms of mechanical properties, biological function, or thermal compatibility. The mechanical properties of the myocardial injury repair patch prepared by this invention can precisely match those of natural myocardium, while possessing high cell activity, effectively inhibiting fibrosis, improving myocardial function, and achieving true active repair. Furthermore, the process conditions are controllable, demonstrating good translational potential.

The invention discloses staphylococcus warneri CCSM003 separated from human skin and capable of repairing a skin barrier and a metagen of the staphylococcus warneri CCSM003, and belongs to the field of microbial technologies and medicines. The staphylococcus warneri CCSM003 provided by the invention can be fermented to generate the L-glycine-valine dipeptide, and the staphylococcus warneri CCSM003 has a good effect of repairing a barrier function in the aspect of external use and an application prospect, specifically, the activity of damaged HaCaT cells is improved in vitro; the gene level expression of FLG, IVL, AQP3, ZO-1, CLDN and Occludin can be improved at the same time in the aspect of improving functional protein and structural protein after damage. Therefore, the staphylococcus warneri has a huge application prospect in preparation of external cosmetics or medicines for repairing the skin barrier.

The invention discloses a gamma-PGA synthetase gene cluster pgsBCA '. The gamma-PGA synthetase gene cluster pgsBCA' is formed by sequentially connecting genes pgsB, pgsC and pgsA 'in series, the genes pgsB and pgsC are derived from a gamma-PGA synthetase gene cluster BA-pgsBCA of the bacillus altitudinis; the gene pgsA'is derived from a gamma-PGA synthetase gene cluster BM-pgsB 'C' A 'of bacillus megatherium. Bacillus amyloliquefaciens CF of which a genome knockout polyglutamate synthetase gene cluster is used as a host, and a recombinant polyglutamate synthetase gene cluster pgsBCA'is subjected to free expression through plasmids, so that a gene engineering strain capable of synthesizing gamma-L-PGA is obtained. When the strain is used for producing gamma-L-PGA in a 5L fermentation tank through amplification fermentation, the maximum yield can reach 10.5 g / L. Besides, the gamma-poly-L-glutamic acid is free of cytotoxicity and low in immunogenicity, so that the gamma-poly-L-glutamic acid has a wide application prospect in the fields of preparation of wound dressings, in-vivo absorption materials and the like. The invention provides a new material basis and technical support for application and development of polyglutamic acid in the fields of medicine and related biomedicine.

The invention provides a composition for preventing bone loss caused by cold exposure as well as a preparation method and application of the composition, and belongs to the technical field of pharmaceutical compositions. The composition provided by the invention is prepared from the following components in parts by weight: 1.18 to 1.58 parts of radix angelicae sinensis extract, 1.18 to 1.58 parts of flos carthami extract, 1.18 to 1.58 parts of semen persicae extract and 2.56 to 2.96 parts of fructus crataegi extract. The preparation method of the composition comprises the following steps: weighing the polymer carrier, adding the polymer carrier into precooled purified water, and adding ethanol; weighing a raw material mixture, and adding the raw material mixture into the polymer solution; performing spray drying to obtain fine powder, and performing vacuum drying to obtain the composition for preventing bone loss caused by cold exposure. The composition for preventing bone loss caused by cold exposure, prepared by the invention, has high solubility and high dissolution rate, and active ingredients take effect quickly; the bone loss caused by cold exposure can be prevented.

The present invention discloses a cyclic peptide and its application. Specifically, the present invention provides a cyclic peptide as shown in formula I. The cyclic peptide provided by the present invention has good biological stability and good cell activity, and is suitable for clinical promotion and application.

The invention belongs to the technical field of stably transfected cell strains, and particularly relates to an APPswe695-overexpressed mouse hippocampal neuron cell strain as well as a construction method and application thereof, the mouse hippocampal neuron cell strain HT22-APPswe695 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: C2024239, and the preservation number is CGMCC No: C2024239. The construction method of the cell strain disclosed by the invention comprises the following steps: constructing plasmids of the overexpressed APPswe695 gene and transfecting the plasmids of the overexpressed APPswe695 gene; the method comprises the following steps of: harvesting, concentrating and purifying lentivirus, and screening by using puromycin after lentivirus infection to obtain a cell strain HT22-APPswe695 overexpressing the APPswe695 gene; the HT22-APPswe695 stably transfected cell strain constructed in the invention has Alzheimer's disease cell level molecular expression characteristics and strong cell viability, and can be used as a tool for in-depth research on the molecular mechanism of Alzheimer's disease and intervention research.

The invention discloses an application of a degradable block copolymer in the prevention and control of atmospheric particulate pollution. The block copolymer can effectively adsorb harmful substances in atmospheric particulate matter, especially can effectively adsorb negatively charged harmful substances in atmospheric particulate matter, thereby playing the role of adsorption and detoxification, and reducing the damage of atmospheric particulate pollutants to human body; it can also significantly reduce the toxic effect of atmospheric particulate pollutants on human myocardial cells or human bronchial epithelial cells, alleviate the damage caused by atmospheric particulate pollutants to human myocardial cells or human bronchial epithelial cells, and alleviate the excessive generation of ROS in human myocardial cells or human bronchial epithelial cells caused by PM. Specifically, the cell activity of the PM+PHB-P group is at least 10% higher than that of the PM group, the cell apoptosis rate of the PM+PHB-P group is significantly lower than that of the PM group by at least 43%, and the cell ROS of the PM+PHB-P group is significantly lower than that of the PM group by at least 13%.