14results about How to "Proliferate fast" patented technology

The invention discloses a prothallus proliferation culture medium and a rapid propagation method for rare and endangered plant cibotium barometz. The prothallus proliferation culture medium of cibotium barometz is used for accelerating the proliferation of gametophytes, and the prothallus maturation time is shortened; meanwhile, the invention provides a rapid propagation method of cibotium barometz, which comprises the following steps: carrying out artificial sterile culture on cibotium barometz spores, rapidly germinating in a culture bottle to form prothalli, culturing on a prothalli proliferation culture medium, inducing and transforming gametophytes in a specific matrix outside the bottle to form sporophytes by virtue of an incomplete tissue culture method, and transplanting. According to the method, the formation of prothallium is accelerated, the sporophyte conversion rate is increased, the reproduction rate is increased while the domestication and reproduction of sporophyte seedlings are accelerated, a large number of cibotium barometz seedlings which grow healthily can be obtained, the survival rate reaches 90%, the spore breeding period is obviously shortened, the operation requirement is not strict, and the method is suitable for large-scale popularization and application. The technical problems that the seedlings in the tissue culture bottle are easy to pollute, fragile and low in seedling rate are solved.

The invention discloses a process for treating rural domestic sewage by using an improved A2 / O + MBBR (Moving Bed Biofilm Reactor), and relates to the technical field of sewage treatment. The method comprises the steps of S1, initial-stage treatment, S2, reflux treatment and S3, tail-stage treatment. The rural domestic sewage is treated by an improved A2 / O + MBBR nitrogen and phosphorus removal process, and the rural domestic sewage is distributed into each tank by adopting a sectional water inlet technology, so that an alternate multi-stage facultative / aerobic environment is formed, and the biological nitrogen and phosphorus removal effect is enhanced; the improved A2 / O + MBBR process has the advantages of high nitrogen and phosphorus removal effect, low investment and operation cost, convenience in operation and management and the like; all returned sludge enters the pre-denitration tank and enters the front-section anaerobic tank and the front-section anoxic tank, a sludge concentration gradient from high to low is formed in each tank, and the average sludge concentration in each tank is increased and the hydraulic load of the sedimentation tank is not changed under the condition that the effluent sludge concentration is not increased, so that the proliferation speed of phosphorus-accumulating bacteria and nitrifying bacteria is accelerated; the proportion in the total amount of the activated sludge is increased, so that the nitrogen and phosphorus removal effect is improved.

The invention provides a co-culture system for increasing the yield of endometrial stem cell exosomes and a method for increasing the yield of endometrial stem cell exosomes, firstly, fibroblasts are subjected to mitomycin C pretreatment, so that the cells are stagnated in G1, S and G2 stages of a cell cycle, meanwhile, RNA and protein synthesis continues to be carried out, and the endometrial stem cell exosomes are obtained; the fibroblasts are kept in a non-proliferative but metabolic active state; then, endometrial stem cells extracted from endometrial tissues in the proliferation period and fibroblasts are indirectly co-cultured through a Transwell chamber and are separated through a semi-permeable membrane, and stem cell differentiation caused by inter-cell contact reaction and fusion of the stem cells and somatic cells is eliminated; after co-culture, taking out the endometrial stem cells, putting the endometrial stem cells into a serum-free culture medium special for exosomes for separate culture, harvesting cell supernate, and purifying the exosomes; compared with the endometrial stem cells which are independently cultured in the whole process, the co-culture system disclosed by the invention has the advantages that the amplification speed of the endometrial stem cells and the exosome yield of unit quantity of cells are remarkably improved.

The application discloses a method for inducing T cell reprogramming into NK-like cells, comprising the following steps: (1) culturing the T cells in a culture system to obtain activated T cells; (2) adding small molecule inducers twice to the activated T cells to obtain NK-like cells; wherein the culture system in step (1) contains 1-10 v / v % serum substitute and / or platelet lysate, and does not contain fetal bovine serum. By optimizing the culture process and precisely adding drugs twice, the small molecule accumulation toxicity caused by multiple drug additions is avoided, the damage to the structure and function of the mitochondrial membrane is reduced, the mitochondrial membrane potential is significantly improved, the energy metabolism capacity of the mitochondria is enhanced, sufficient energy support is provided for the long-term survival and function maintenance of the cITNK cells, and the physiological activity of the cells is ensured from the energy metabolism level.

The invention belongs to the field of daily chemicals, and discloses a repairing and nourishing composition for shower gel, which comprises the following components: first composite ceramide, second composite ceramide, nicotinamide, citric acid, vegetable fat and caffeine, the vegetable fat contains frankincense oil. According to the composition, two kinds of composite ceramide and nicotinamide are used for forming eutectic crystals, the first composite ceramide is used for forming a skin lipid barrier and maintaining a skin lipid double-layer structure, and the second composite ceramide is used for improving the deep moisturizing capacity of the skin and enhancing the elasticity of the skin; meanwhile, the frankincense oil is used, the effect of ceramide acting on the skin can be further improved, citric acid and caffeine are used in cooperation, cutin updating is achieved on the basis of the functions, the cell proliferation speed is increased, the cell viability is improved, and the effect of comprehensive skin repairing is achieved. Another purpose of the invention is to provide application of the composition and shower gel.

Active components of the mixed fruit powder, polypeptide and probiotic composition comprise the following components in parts by weight: 45-60 parts of mixed fruit powder, 30-50 parts of polypeptide and 5-25 parts of probiotics, wherein the mixed fruit powder comprises a mixture of two or more of cherry fruit powder, roxburgh rose fruit powder, cranberry fruit powder, pomegranate fruit powder, sea buckthorn fruit powder and mulberry fruit powder; the polypeptide comprises one or more of oyster peptide, sea cucumber peptide or krill peptide; the probiotics comprise one or more of lactobacillus paracasei JLPF-131, lactobacillus acidophilus JYLA-191 or bifidobacterium lactis JYBR-390, and the probiotics comprise one or more of lactobacillus paracasei JLPF-131, lactobacillus acidophilus JYLA-191 and bifidobacterium When the probiotic food or health-care product is developed, the requirements of diarrhea patients are considered, the use of prebiotics is avoided as much as possible, and the reproduction of the probiotics can also be ensured.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The invention relates to the technical field of cell models, and discloses a method for inducing osteoclast in vitro from mouse mononuclear macrophage leukemia cells, which comprises the following steps: S10: passage of mouse mononuclear macrophage leukemia cells: inoculating the mouse mononuclear macrophage leukemia cells into a DMEM (dulbecco's modified eagle medium) high-glucose complete medium containing special-grade fetal calf serum; performing passage; s20, inducing mouse mononuclear macrophage leukemia cells: collecting 1-5 generations of mouse mononuclear macrophage leukemia cells, inoculating the mouse mononuclear macrophage leukemia cells into a DMEM high-glucose complete medium containing special-grade fetal calf serum, and adding a nuclear factor kappa B receptor activating factor ligand into the medium after 20-24 hours; s30, continuously culturing for 40-48 hours, replacing the culture medium, and adding a nuclear factor kappa B receptor activating factor ligand at the same time; and S40, continuing to culture for 40-48 hours to obtain mature osteoclasts, so that the in-vitro osteoclast induction efficiency of RAW264.7 cells can be improved.

The present invention discloses an epidermal organoid culture medium and a culture method, belonging to the field of organoid technology. The epidermal organoid culture medium includes: a basal culture medium, a specific additive factor, CHIR99021 and Forskolin; wherein the specific additive factor includes the following components at the final concentration: Glutamax, 0.5-2×; HEPES, 5-20mM; N-Acetylcysteine, 1-5mM; Nicotinamide, 1-20mM; Penicillin streptomycin, 0.5-5×; B27, 0.5-2×, etc. The culture medium of the present invention can increase the proliferation rate of epidermal organoids, shorten the construction time of epidermal organoids, increase the construction success rate of epidermal organoids, and the sample is easy to obtain, the performance of the epidermis can be guaranteed, and it is easy to industrialize production.

The invention provides a complex microbial inoculant for fermenting soybean meal and application of the complex microbial inoculant. Strain in the complex microbial inoculant for fermenting soybean meal comprises lactic acid bacteria, bacillus and saccharomycetes; the lactic acid bacteria comprise lactobacillus plantarum and pediococcus acidilactici; the bacillus comprises bacillus coagulans and bacillus subtilis. The five strains of lactobacillus plantarum, pediococcus acidilactici, bacillus coagulans, bacillus subtilis and saccharomycetes are combined for use, so that macromolecular protein in a substrate can be effectively decomposed, antinutritional factors are reduced, and the flavor of soybean meal is improved; the fermented soybean meal has a synergistic effect in the aspects of increasing the viable count of the fermented soybean meal product, increasing the weight of fed animals and reducing the diarrhea rate of the fed animals. The complex microbial inoculant for fermenting the soybean meal is used for fermenting the soybean meal, is high in microbial proliferation speed, high in viable count in a final product, good in flavor and low in fermentation cost, and has higher economic application value.

The present invention relates to the field of cell culture technology, and in particular to a CIK cell culture method and application thereof. The culture method comprises the following steps: (1) isolating peripheral blood mononuclear cells (PBMC); (2) preparing culture medium A; (3) preparing culture medium B; (4) resuspending and inoculating peripheral blood mononuclear cells; and (5) collecting cell culture. The culture method of the present invention can improve the yield of CIK cells, and wherein CD3 + CD56 + The proportion of double-positive cells is high, which increases cell proliferation activity and promotes the increase of CIK cell proliferation and killing activity.

The present invention provides an RA autoantigen epitope polypeptide and an antigen-specific T cell vaccine prepared therefrom, a preparation method and an application thereof, and belongs to the technical field of immunotherapy. The present invention modifies the N-terminus and the C-terminus and transforms specific amino acids to make the RA autoantigen epitope polypeptide CII 1237‑1249 It has good stimulating effect and prolonged half-life. 1237‑1249 The peptide was used as an autoantigen epitope peptide for rheumatoid arthritis, combined with IL-7 and IL-15 as well as IL-2 and PHA stimulation, and then inactivated and flow-sorted antigen-specific CD4 + T cells are obtained to obtain an antigen-specific T cell vaccine that does not proliferate but retains immunogenicity. After the antigen-specific T cell vaccine is subcutaneously injected, it can effectively correct immune disorders and achieve the purpose of treating rheumatoid arthritis. The present invention provides a safe and reliable new method for the treatment of rheumatoid arthritis.

The invention discloses a culture method of a high-yield rabies vaccine virus harvesting liquid, belongs to the technical field of vaccines, and solves the problems of low rabies vaccine yield, high cost and inconsistent product quality in the prior art. The method comprises the following steps: resuscitating human diploid working library cells by adopting a culture bottle, and amplifying step by step until the cells are subcultured to a cell factory; and inoculating the cells into a bioreactor for microcarrier culture, after the cells grow compactly, subculturing the cells into the bioreactor for flaky carrier culture, after the cells on the flaky carrier grow compactly, inoculating rabies virus, and then harvesting virus liquid. According to the method, on one hand, the defects of long passage time and large workload when cells are cultured by only using a culture bottle are avoided; on the other hand, the lamellar carrier is used for performing final cell culture and virus culture, so that the cells can be prevented from being influenced by stirring shearing force, the harvesting time of the viruses is prolonged, and the harvesting amount of the viruses is increased.

The invention relates to the technical field of tissue culture of dysosma versipellis, in particular to a tissue culture medium combination and a tissue culture rapid propagation method of dysosma versipellis. The invention provides a dysosma versipellis tissue culture medium combination and a tissue culture rapid propagation method.The dysosma versipellis tissue culture medium combination comprises a germination culture medium, a callus induction culture medium, a cluster bud proliferation culture medium and a rooting culture medium, the callus induction rate can be increased, and the tissue culture rapid propagation rate can be increased. The inductivity and the multiplication coefficient of the multiple shoots of the dysosma versipellis are improved, and the rooting rate of tissue culture seedlings of the dysosma versipellis is improved. According to the tissue culture rapid propagation method for the dysosma versipellis, the dysosma versipellis is high in propagation speed, short in production period, simple in equipment and convenient for industrialized production, and guarantee conditions are provided for production of authentic medicinal materials and postpartum processing and selling.