The invention relates to a method for producing the lycoris by plant tissue culture, which comprises the steps of: (1) taking an MS (mass spectrometry) culture medium as a basic culture medium, and taking a 2, 4-D, IAA (indo acetic acid), NAA (naphthyl acetic acid), 6-BA (butyl acrylate) and IBA (iso butyl alcohol) as a regulated and controlled growth hormone preparation culture medium; (2) taking the bulb of the lycoris as an explant, and sterilizing; (3) vaccinating the explant of the bulb on a bulb inducing culture medium to culture in an inducing way so as to grow bulbils; (4) transferring the bulbils into a rooting culture medium to culture in a rooting way, so that the bulbils can independently absorb the nutrient elements from the soil and the matrix; (5) transferring the bulbils into the culture medium to culture; (6) opening an opening of a vaccination bottle to refine the seedling; withdrawing the bulbils, and transferring the bulbils into a nutrient cup which takes the vermiculite, the pearlite and the nutrient soil as the mixed matrix; and transferring the bulbils into a large tent, so that the survival rate reaches 94%-96%. The method is less in material taking, and economical in culturing; the culture condition can be manually controlled and is not influenced by the natural condition; the method is short in growing period, and high in propagation rate; and the quality of lycoris is guaranteed.