Method for producing lycoris by plant tissue culture

A technology for plant tissue culture and lycoris, which is applied to the field of plant tissue culture, can solve the problems of unfavorable quality continuation and development, low reproduction rate of lycoris, and pathogens, and achieves easy automatic control, expansion of reproduction, and short growth cycle. Effect

Inactive Publication Date: 2011-08-10
江苏九久环境科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a method for producing Lycoris by plant tissue culture, to solve the problem that Lycoris is low in reproduction rate and contains germs, so that the variety i

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  • Method for producing lycoris by plant tissue culture

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Embodiment 1

[0021] Embodiment one: a kind of method adopting plant tissue culture lycoris, comprises the following steps:

[0022] (1) Preparation of medium: MS medium (Murashige and Skoog, 1962) was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare three kinds of medium respectively : Bulb induction medium A: MS+NAA mg / L+2, 4-D 0.2mg / L+6-BA 1.0mg / L, rooting medium B: MS+IAA 0.1mg / L+IBA 0.1mg / L , medium C: MS+IAA 0.1mg / L; 20g / L sucrose and 4g / L agar were added to medium A and B, 15g / L sucrose and 1g / L agar were added to medium C, and 1mol L -1 Adjust the pH to 5.8 with NaOH and HCL, and sterilize the medium A, B and C in a high-pressure steam sterilizer at 0.1MPa and 121°C for 15min;

[0023] (2) Disinfection of explants: select Lycoris bulbs as explants, wash them with tap water for 1 hour, disinfect them with 0.5% 84 for 4 minutes on the aseptic operating table, rinse them with sterile water for 4 times, and then wash them...

Embodiment 2

[0028]Embodiment two: a kind of method adopting plant tissue culture Lycoris, comprises the following steps:

[0029] (1) Preparation of medium: MS medium (Murashige and Skoog, 1962) was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare three kinds of medium respectively : Bulb induction medium A: MS+NAA 2.0mg / L+2,4-D 1.0mg / L+6-BA 5.0mg / L, rooting medium B: MS+IAA 1.0mg / L+IBA 1.0mg / L L, medium C: MS+IAA 1.0mg / L; 35g / L sucrose and 5g / L agar were added to medium A and B, 25g / L sucrose and 2g / L agar were added to medium C, and 2mol· L -1 NaOH and HCL to adjust the pH to 6.0, and sterilize the medium A, B and C in a high-pressure steam sterilizer at 0.15MPa and 125°C for 20min;

[0030] (2) Disinfection of explants: choose Lycoris bulbs as explants, wash them with tap water for 2 hours, disinfect them with 1% 84 for 2 minutes on the aseptic operating table, rinse them twice with sterile water, and then wash them wit...

Embodiment 3

[0035] Embodiment three: a kind of method adopting plant tissue culture Lycoris, comprises the following steps:

[0036] (1) Preparation of medium: MS medium (Murashige and Skoog, 1962) was selected as the basic medium, and 2,4-D, IAA, NAA, 6-BA and IBA were used as regulated growth hormones to prepare three kinds of medium respectively : Bulb induction medium A: MS+NAA 0.6mg / L+2, 4-D 0.3mg / L+6-BA 1.2mg / L, rooting medium B: MS+IAA 0.5mg / L+IBA 0.2mg / L L, medium C: MS+IAA 0.2mg / L; medium A and B were supplemented with 25g / L sucrose and 4.5g / L agar, medium C was supplemented with 16g / L sucrose and 1.2g / L agar, used 1.2mol·L -1 NaOH and HCL to adjust the pH to 5.9, and sterilize the medium A, B and C in a high-pressure steam sterilizer at 0.12MPa and 122°C for 16min;

[0037] (2) Disinfection of explants: select Lycoris bulbs as explants, wash them with tap water for 1.2 hours, disinfect them with 0.6% 84 for 3 minutes on the sterile operating table, rinse them with sterile wate...

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Abstract

The invention relates to a method for producing the lycoris by plant tissue culture, which comprises the steps of: (1) taking an MS (mass spectrometry) culture medium as a basic culture medium, and taking a 2, 4-D, IAA (indo acetic acid), NAA (naphthyl acetic acid), 6-BA (butyl acrylate) and IBA (iso butyl alcohol) as a regulated and controlled growth hormone preparation culture medium; (2) taking the bulb of the lycoris as an explant, and sterilizing; (3) vaccinating the explant of the bulb on a bulb inducing culture medium to culture in an inducing way so as to grow bulbils; (4) transferring the bulbils into a rooting culture medium to culture in a rooting way, so that the bulbils can independently absorb the nutrient elements from the soil and the matrix; (5) transferring the bulbils into the culture medium to culture; (6) opening an opening of a vaccination bottle to refine the seedling; withdrawing the bulbils, and transferring the bulbils into a nutrient cup which takes the vermiculite, the pearlite and the nutrient soil as the mixed matrix; and transferring the bulbils into a large tent, so that the survival rate reaches 94%-96%. The method is less in material taking, and economical in culturing; the culture condition can be manually controlled and is not influenced by the natural condition; the method is short in growing period, and high in propagation rate; and the quality of lycoris is guaranteed.

Description

technical field [0001] The invention relates to a method for producing lycoris by adopting plant tissue culture and belongs to the technical field of plant tissue culture. Background technique [0002] There are more than 20 kinds of Lycoris plants in the world, mainly distributed in China and Japan, and a few in Myanmar and North Korea. There are 16 species in my country, mainly distributed in the south of the Yangtze River. This genus of plants likes to be born beside the rocky rocks in relatively damp streams. The bulbs are covered with dark brown membranous scales, and the leaves are flat and long linear or narrow linear, resembling garlic, hence the name. Lycoris plants have long been used as medicinal plants to treat diseases and injuries. According to "Compendium of Materia Medica" and other records, Lycoris has detoxification, expectorant, diuretic, emetic and other effects. Edema etc. Lycoris bulbs contain lycorine, lycorine, galantamine, lycorine, polyflora, dihy...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘浩
Owner 江苏九久环境科技有限公司
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