63results about How to "Large amount of reproduction" patented technology

The invention provides a method for performing tissue culture and rapid propagation on primula saxatilis. The method comprises the following steps of: selecting wild primula saxatilis seeds collected from the Mountain Miyunwuling in Beijing as explants; sterilizing the seeds and then performing inoculation culture on the seeds, so that the seeds sprout to form sterile seedlings of the primula saxatilis; performing propagation on axillary buds on the sterile seedlings and then performing rapid propagation through cluster bud induction and subculture; or generating callus by inducing leaves or petioles, performing propagation on the callus and performing adventitious bud differentiation to perform rapid propagation. By the method for performing tissue culture and rapid propagation on the primula saxatilis provided by the invention, the seedling-cultivating production capacity of the primula saxatilis is greatly improved, seedling time and cost are reduced, and technical guarantee is provided for the storage of excellent individual plants of the primula saxatilis.

The invention discloses an agasicles hygrophila experimental population raising method. The agasicles hygrophila experimental population raising method includes steps that filter paper with the size equal to that of a bottle bottom is placed in an insect breeding bottle I, and is wetted, and 20-30 adult insects which are just subjected to the eclosion are placed in the insect breeding bottle I and are fed by three fresh alternanthera philoxeroides; alternanthera philoxeroides leaves with egg masses in the insect breeding bottle I are placed in insect breeding bottles II, each insert breeding bottle II holds ten alternanthera philoxeroides leaves, and then the insect breeding bottles II are placed in an artificial incubator; fresh alternanthera philoxeroides is placed in the incubator on the first day of incubation, and the alternanthera philoxeroides is continuously replaced by other fresh alternanthera philoxeroides until larvas are matured; and when about to pupate, the agasicles hygrophila matured larvas are transferred into pupating devices, each pupating device contains 100 matured larvas, the matured larvas enter straws of alternanthera philoxeroides to pupate, and pupating rate can reach 80% to 95%. The agasicles hygrophila experimental population raising method is simple in artificial raising, easy to control, small in occupied space and low in cost, and can be used for raising a controllable quantity of neat and consistent agasicles hygrophila with different insect development stages and different insect ages according to different experimental needs.

The invention relates to an efficient and rapid tissue culture propagation method of konjac. The efficient and rapid tissue culture propagation method comprises the following steps of: carrying out isolated culture on a konjac corm slicing material and inducing to generate callus, adventitious buds and adventitious roots; and further forming a great mount of konjac tissue culture seedlings within a very short period of time; transplanting the tissue culture seedlings, necessary medicals and fertilizers to a seedbed; and tumbling sprouts normally, and then obtaining a konjac original seed. The efficient and rapid tissue culture propagation method of the konjac, provided by the invention, overcomes the defect that the konjac is incapable of integrating with the market due to low traditional propagation coefficient and high industrial rapid propagation cost, has high propagation coefficient, large propagation amount, a primary pollution rate less than 10% and an average inductivity higher than 90%, shortens a concentrated seedling cycle to three and a half months, can be used for producing seedlings all the year around, producing the original seed from March to July each year under natural conditions, producing the original seed in other time under artificial greenhouse conditions and obtaining the original seeds with an average weight up to 10g or more, greatly lowers the production cost and improves the production benefit.

The invention belongs to the field of plant tissue culture of plant cell engineering techniques, and in particular relates to a tissue culture propagation method for reducing the tissue culture browning rate of traxacum koksaghyz. The tissue culture propagation method comprises the following steps: inoculating, namely, selecting mature seeds of a traxacum koksaghyz strain; performing induced differentiation on calluses; inducing callus formation; proliferating adventitious buds; performing rooting culture; separating multiple shoots into single plants, and transferring to a rooting culture medium for rooting culture; performing acclimatization and transplantation, thereby obtaining regenerated plants of traxacum koksaghyz. By adopting the tissue culture propagation method, a method for effectively reducing the tissue culture browning rate of traxacum koksaghyz and rapidly propagating traxacum koksaghyz is provided, the tissue culture browning rate of traxacum koksaghyz is greatly reduced through technical measures such as adjusting components of culture mediums used in different stages and adding an antioxidant, the propagation coefficient of traxacum koksaghyz is improved, the browning rate can be reduced to be less than 35% when the culture mediums provided by the invention is adopted, and the browning rate can be further reduced to be less than 10% when Na2S2O3 is added.

The invention relates to a method for plant leaf bud shoot cutting propagation all year around and relates to the technical field of vegetative propagation. The method aims to overcome the defects that in the prior art, nursery stock seedling propagation is large in variation and part of nursery stock is low in maturing rate, and tissue culture propagation is high in cost and difficult in technology management, and minimum stems of existing fine seed nursery stock leaf buds are adopted as female parent cuttage materials for cuttage. The method includes the steps of solar greenhouse or movable greenhouse facility and planting bed preparation, leaf bud selection, cuttage time and method determination and seedling period management. The propagation coefficient of seedlings cultivated in the method can be progressively increased in a multiplied mode, the survival rate of the seedlings can reach 90%, roots are developed and grow vigorously, and the transplantation survival rate can exceed 95%. The method has the greatest advantages that mass production and industrialized production can be achieved, propagation does not need professionals, and the method is easy to operate, low in individual cost, high in propagated seedling survival rate and easy to popularize.

The invention discloses a toothbrush and a preparation method thereof. The toothbrush comprise a brush handle and a cleaning part, wherein the brush handle comprises a handheld part and a brush head part, the handheld part is connected with the brush head part, and the cleaning part is fixedly connected with the brush head part; the cleaning part comprises a plurality of brush hairs and a hair plate, the hair plate is formed through integral fusion of the bottom of the plurality of brush hairs, and the hair plate and the brush head part are fixedly connected. The toothbrush has the beneficial effects that the plurality of brush hairs are tightly arranged and the bottom of the brush hairs is fused as the hair plate, the unfused part stands erectly on the hair plate, the integral adhesion force of the brush hairs is enhanced, the direct cleaning area of the brush hairs and the teeth is increased at the same time, and the toothbrush is in favor of the cleaning of the teeth; furthermore. The toothbrush has soft brush hairs, can bring comfort feeling for the user while being used for cleaning the teeth, and is more in favor of the children, pregnant, and oral disease patient with gingiva easy to blood after friction. Furthermore, the toothbrush is simple in preparation process and low in cost.

The invention relates to a method for quickly cultivating the orange lamp table (Primula bulleyana), comprising explant selection, surface disinfection, adventitious induction, generation cultivation, rooting cultivation, and tube seed transfer. With said invention, one axillary bud can be induced into 40-50 buds, while the increment factor is 3-4 via 30-40 days of generation and the rooting rate can reach 95%, and the yield can reach 100%. The invention can improve the seeding ability of orange lamp table (Primula bulleyana), shorten the seed time and reduce the cost.

The present invention relates to a pteridophyte propagation method in limestone district, belonging to the field of flowers, plants and Chinese medicinal herbs cultivation technology. Said method includes the following steps: collecting spores in spore ripening season of pteridophyte, placing the collected spores into a clean container, placing said container in a ventilating shade place and making it be naturally dried; in 2-3 month of spring sowing the spores into the moss cluster grown on the nightside limestone, after the sporophyte is grown for 20-30 days, transplanting the sporophyte together with moss cluster into green-house or flowers nursery garden to make cultivation.

The invention discloses a method for rearing parent fishes of ictalurus punctatus. The method comprises the following steps of (1) selecting high-quality parent fishes and putting the high-quality parent fishes into a breeding pond; (2) after the parent fishes are stocked for 5-10 days, starting breeding and feeding, and using fresh fish blocks for feeding from 30 days before spawning of the parent fishes to the breeding period; (3) during the breeding period of the parent fishes, inspecting the activity conditions of the parent fishes late night every day, and when it is found that the parent fishes have chasing reactions on the water surface, putting artificial fish nests into the pond for the parent fishes to lay eggs; and (4) taking out egg masses in the artificial fish nests, putting the egg masses into a water basin filled with pond water, and feeding the egg masses into a hatching room for hatching under the condition of avoiding direct sunlight. The technology for rearing the parent fishes of the ictalurus punctatus is simple and easy to operate, has good promotion effects on optimizing the breeding process, reducing the labor amount and improving the survival and reproductive capacity of the parent fishes, and has good economic benefits and social benefits.

The invention discloses a cryptomeria fortunei tissue culture rapid-propagation method. The method comprises taking a cryptomeria fortunei explant stem segment, inoculating the cryptomeria fortunei explant stem segment on an inducing adventitious bud culture medium which comprises DCR+ 0.1-2.0mg/L of 6-BA+ 0.1-2.0mg/L of IBA+ 0.5-5.0g/L of AC+ 0.001-0.01mg/L of TDZ+ 30g/L of cane sugar, and performing inducing culture to generate adventitious buds; adopting two stages to take root for rooting culture, namely first placing a rooting culture medium 1 which comprises 1/2 of DCR+ 0.1-2.0mg/L of NAA+ 0.1-2.0mg/L of IBA+0.1-1.0mg/L of 6-BA+ 1.0-10.0g/L of AC+ 10.0-30.0g/L of cane sugar, first performing dark culture for 2-15d, performing culture for 5-30d under illumination, and transferring to a rooting culture medium 2 which comprises DCR+ 0.1-1.0mg/L of NAA+ 0.1-1.0mg/L of 6-BA+ 0.5-5.0g/L of AC+ 30.0g/L of cane sugar. By the method, the tender bud propagation coefficient is over 5.7, and the rooting rate is over 80%. The culture cycle is short, the propagation quantity is large, and the cryptomeria fortunei tissue culture rapid-propagation method is favorable for large-scale production.

The invention discloses a method for producing feed through enzyme-linked micro-ecologic solid state fermentation of vinasse. The method comprises the following steps: 1, carrying out primary fermentation: drying fresh vinasse until the water content is 10-25%, adding wheat bran and a meal component, mixing the dried vinasse, the wheat bran and the meal component to form a fermentation raw material, inoculating the fermentation raw material with a working fluid, adding a probiotic compound enzyme, placing the obtained mixture in a single-hole breathing bag, and carrying out enzymatic hydrolysis fermentation to obtain the biological feed; and 2, carrying out semi-conservative continuous fermentation: taking a part of the biological feed to substitute the working fluid in step 1, and repeating the step 1 to continuously obtain the biological feed. The proper animal probiotic strain and the compound enzyme are selected, and the semi-conservative continuous fermentation is carried out, so nutrients and precursors are supplemented, and metabolized harmful substances are diluted, thereby continuous synthesis of the above product and further degradation of anti-nutrition factors are facilitated, the nutrition values of the vinasse are greatly improved, the added values of the vinasse are increased, the storage and long-distance transportation of the feed are convenient, and the method has good social and economic benefits and environmental protection meaning.

The invention relates to the technical field of cosmetic efficacy evaluation, and discloses a method for evaluating the efficacy of cosmetics for relieving or repairing skin inflammation in order to solve the problems that in an existing cosmetic skin inflammation relieving evaluation method, the subjectivity of evaluation index selection and treatment is high, and subjective factor interference is brought to an evaluation result. The method comprises the following steps: taking zebra fish as a test object, setting a normal control group, a model control group and a test group, culturing underthe same condition, photographing under a fluorescence microscope, storing a picture, and counting the number of neutrophils in a skin inflammation part of the zebra fish by adopting image processingsoftware based on a statistical method; and then calculating the inflammation relieving or repairing effect (%). Compared with the prior art, the evaluation index data is selected and analyzed by means of instruments and software, and the evaluation result can be quantified; the zebra fish breeding cost is low, the breeding period is short, the breeding amount is large, and the evaluation methodhas the advantages of being efficient, rapid and low in cost.

The invention relates to the propagation of Sargassum thunbergii, in particular to a method for massively propagating the seedlings of the Sargassum thunbergii by a tissue culture vane method, comprising the steps of: 1) picking the complete principal branches in the sample of the Sargassum thunbergii and using disinfected seawater to disinfect; 2) then using AgCl to disinfect and process; using a bistoury to take down the wide and large vanes in the middle lower part of the principal branches and using a nipper to arrange the wide and large vanes into prepared culture liquid; the culturing temperature is between 13 and 20 DEG C; the illumination intensity is 15 to 50 Mumol/m2 per s; and the illumination: darkness in the illumination period is equal to 8 to 10: 16 to 14h; 3) needing not to ventilate 7 to 10 days before culturing; after the 7 to 10 days, beginning to ventilate and culture after the wound healing of the vanes is finished; changing the culture liquid every 35 to 45 days; after culturing for 3 months, a plurality of buds are generated in the middle lower parts of the vanes; and after culturing for 5 months, regenerated strains can be collected to carry out fixed planting and culturing. The method needs less plant materials and less culturing space, can carry out idioplasm preservation for a long period, and has large fecundity and pure seedling quality, and the like.

The invention relates to a method for quickly cultivating the Primula beesiana, which comprises that: selecting the axillary bud of Primula beesiana as explant, to be treated via adventitious induction, generation cultivation, rooting cultivation, and tube seed transfer. With said invention, one axillary bud can be induced into 40-50 buds, while the increment factor is 3-4 via 30-40 days of generation and the rooting rate can reach 95%, and the yield can reach 100%. The invention can improve the seeding ability of orange lamp table (Primula beesiana), shorten the seed time and reduce the cost.

The invention belongs to the technical field of recovering and protecting Gymnocypris przewalskii ecological resource, in particular relating to an anadromous fish pass channel for Gymnocypris przewalskii. The fish pass channel is characterized by being constructed by a plurality of metal boxes, the lower part of each metal box is provided with a support, a fishway dummy plate is arranged in each metal box, the ratio of the total length of the fish pass channel to the vertical height from the lower channel water face to the upper channel water face is 10:0.5-1.5, the highest point of the fishway dummy plate is 8-15cm lower than that of the metal box, and the lowest point of the fishway dummy plate is 35-45cm lower than that of the metal box. The channel does not change the natural spawning habit of Gymnocypris, which seems that a stair way is built for freely and easily going to rivers and lakes, thereby greatly reducing the physical consumption and dead number of Gymnocypris, greatly increasing the spawning amount and small fish reproductive output of Gymnocypris, and having significant economical and environmental benefits.

The invention designs a tissue culture and rapid propagation inoculation method of anthurium andraeanum. The tissue culture and rapid propagation inoculation method comprises the following steps of (1) stripping adventitious buds obtained through differentiation of callus tissue of the anthurium andraeanum from the callus tissue, and inoculating the stripped adventitious buds to elongation culture mediums or rooting culture mediums or propagation culture mediums for culture, wherein culture conditions include the temperature being 25 DEG C, the illumination intensity being 1500-2500Lux and illumination every day being 15-17h, and performing 40-60 d of culture so as to obtain healthy anthurium andraeanum tissue culture seedlings; and selecting the healthy anthurium andraeanum tissue culture seedlings which are 2cm or above in plant height and have two stalk nodes or above from the obtained healthy anthurium andraeanum tissue culture seedlings, in inoculation disks, performing transection at a position 2mm higher than first nodes of the stalk nodes from bottoms to tops of the seedling stalks to form upper parts and lower parts, enabling the upper parts to be inoculated to the rooting culture mediums, enabling the lower parts to be inoculated to the elongation culture mediums for continuous culture, so as to obtain the anthurium andraeanum tissue culture seedlings which grow orderly, wherein the culture conditions are the same as those in the step 1. According to the tissue culture and rapid propagation inoculation method disclosed by the invention, the problem that plants are uneven to grow in the tissue culture and rapid propagation process of the anthurium andraeanum can be solved.

The invention discloses a cuttage cultivation method for crape myrtle in summer. The method comprises the following steps: selections of nursery lands; selections and processing of cutting slips; cuttage; insertion of processed cutting slips into seedbeds and watering; and management. The cuttage cultivation method for crape myrtle in summer has the following beneficial effects: the method is easy to operate; branches are saved; the reproduction amount is large; cost is low; the survival rate of cutting slips of crape myrtle is increased; by soaking with use of snake gourd fruit, callus of crape myrtle can be greatly divided; snake gourd fruit soaking liquid is utilized regularly to pour after cuttage of cutting slips; during three to five days of cuttage, irradiation treatment with quantitative dose is utilized during the crucial stage of callus differentiation so that adventitious roots are further differentiated by callus tissue; the rooting rate is increased significantly and the rooting amount is great; root systems grow robust and are uniformly distributed; the overall seedling time is shortened; decomposition of the callus tissue of cutting slips is avoided; the stress resistance of cutting slips is improved; the cuttage survival rate of crape myrtle is high; outplanting speed is high; and outplanting rate is great.

The invention discloses a method for enhancing breeding rate of bamboo rats. The method comprises the following steps: selecting progenies of premature high-productivity larger-individual male bamboo rats and female bamboo rats as the strains; selecting breeding bamboo rats, which can give birth more than 4 times a year and produce more than 4 babies each time, as the strains; adding a proper amount of nutrients according to the growth of the bamboo rats to supplement minerals by feeding bones of pigs, cattle, fishes and crabs to the bamboo rats 2-6 times a week, wherein the bones of pigs and cattle are firstly stewed for 1-3 hours, dried in air, pulverized into powder and fed to the bamboo rats, and 3-6g of the powder is fed to each bamboo rat every time; and temporarily covering the delivery room with a cloth to enable the female rats to give birth in a quiet and dark environment, breeding the female rats with succulent feed, and avoiding adding feed into the delivery room after delivery, thereby avoiding disturbing the baby rats and female rats. The method can effectively enhance the breeding efficiency (breeding quantity) of bamboo rats by 10-15%. The method can effectively prevent accidental death of baby bamboo rats and enhance the survival rate of the baby bamboo rats by 20% or so.

A high-efficiency and rapid tissue culture breeding method of konjac is as below: conducting isolated culture on konjac bulbs to induce callus, adventitious buds and adventitious roots; forming a large number of konjac tissue culture seedlings in a short time; then transplanting tissue culture seedlings in a seedbed; supplementing the seedlings with necessary chemicals and fertilizers; and obtaining a konjac breeder's stock after normal lodging. The high-efficiency and rapid tissue culture breeding method of the konjac provided by the invention overcomes the shortcomings of a low breeding coefficient of traditional konjac breeding, high cost of the whole factory rapid breeding, and failure to adapt to the market; the breeding method provided by the invention has high breeding coefficient,large breeding amount, first generation pollution rate lower than 10%, average induction rate higher than 90%, and a concentrated seedling cycle shortened to 3 and a half months. The seedlings can beproduced on an annual basis, the breeder's stock is produced under natural conditions from March to July every year, and under artificial greenhouse conditions at other times. The breeder's stock hasan average weight of 10 grams or more, greatly reduces the production cost and greatly improves the production efficiency.

The invention discloses a production method of lucid ganoderma livestock immunological competence feed. The lucid ganoderma livestock immunological competence feed contains bioactive constituents and other nutritional ingredients peculiar to lucid ganoderma. After the culturing period of the lucid ganoderma is finished, a culture medium (a lucid ganoderma medium for short) of sporocarp is picked, low temperature vacuum drying and airflow superfine grinding are carried out on the culture medium, the processed culture medium is mixed with livestock conventional feed and then is input in a livestock feed granulator for extrusion granulation, and lastly the lucid ganoderma livestock immunological competence feed is formed. The production method of lucid ganoderma livestock immunological competence feed improves the functions of disease prevention and disease resistance by adjusting the immunologic function of livestock, avoids the problems of ecological environment deterioration and poor quality of livestock meat caused by abuse of antibiotics and other drugs, can improve the utilization rate of the feed, promotes the growth of the livestock, improves the taste of the livestock meat, and has the advantages of being low in production cost, simple in process, wide in use range, safe, efficient, and the like.

The invention discloses a production method of an immunocompetence feed for trepang. The immunocompetence feed for the trepang contains the special bioactive components of cordyceps militaris and other nutrient components, and is prepared through the following steps of completely picking culture mediums (Chinese caterpillar fungus mediums for short) of fruiting bodies after the culture period of the cordyceps militaris is completed, performing low-temperature vacuum drying and air-current ultra-micro crushing, uniformly mixing the crushed powder with a conventional feed, and putting the mixture into a feed granulator for extruding and pelleting. According to the invention, the Chinese caterpillar fungus culture mediums are turned into wealth from waste and are processed into the immunocompetence feed which is used for regulating the immune function of the organism of the trepang and enhancing the disease prevention and disease resistance of animals; the problems that the ecological environment deteriorates, meat quality becomes bad and the like because medicines, such as antibiotics, are abused can be solved; besides, the utilization rate of the feed is increased, the growth of the trepang is promoted, the meat quality and the mouth feel are improved, and favorable economic benefits are created; and waste resources are reused, so that the production method has the advantages of low production cost, simple technology, wide application range, high safety, high efficiency and the like.

The invention discloses a method for cuttage reproduction of a fresh water mangrove plant barringtonia acutangula. The method specifically comprises the following steps: preparing a cuttage bed, preparing scions, treating the scions, performing hormone treatment, performing cutttage, performing after-cuttage management, performing spraying treatment, and performing transplanting. The invention relates to the technical field of plant reproduction cultivation. According to the method for cuttage reproduction of the fresh water mangrove plant barringtonia acutangula, orthogonal experimental designing of three factors and three levels is adopted to combine different hormone soaking concentrations and different soaking times before cuttage of the scions and different hormone spraying concentrations after cuttage, an optimal barringtonia acutangula cuttage reproduction method combination is finally screened, a technical way for large-scale barringtonia acutangula culture can be obtained, anda basis can be made for providing more tree variety options for recovering sea-land ecological systems. By adopting the method, cuttage reproduction growth is rapid, the reproduction amount is large,the cost is low, excellent properties of the variety can be maintained, and technical references can be provided for improved variety seedling breeding for efficient and large-scale reproduction of the barringtonia acutangula.