High-efficiency and rapid tissue culture breeding method of konjac

A technology of konjac and tissue culture, which is applied in the field of high-efficiency and rapid tissue culture propagation of konjac, can solve the problems of high cost of rapid propagation in the whole factory, failure to connect with the market, low reproduction coefficient, etc. The effect of promotion and large reproduction coefficient

Inactive Publication Date: 2019-03-05
刘琳琳
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a high-efficiency and rapid tissue culture propagation method for konjac, which provides a high-efficiency and rapid propagation method for the large-scale production of konjac tissue culture seedlings and its original original seeds in each konjac production area, and overcomes the The shortcomings of konjac's traditional low propagation coefficient, mixed species, low traditional konjac propagation coefficient and high cost of full-factory rapid propagation that cannot be connected with the market have been eliminated, and the original species of konjac varieties has been effectively purified and maintained. It is conducive to the production and promotion of improved varieties, with large reproduction coefficient and large reproduction quantity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of konjac efficient rapid tissue culture propagation method, the method comprises the following steps:

[0049] 1) Preparation of medium: MS medium was used as the basic medium, 2.5mg / L of 6-benzylaminopurine, 2.0mg / L of α-naphthaleneacetic acid, 0.5mg / L of polyvinylpyrrolidone, 30g / L of sucrose, and agar After 6.5g / L, a callus induction medium with a pH value of 5.8-6.0 was prepared,

[0050] Prepared by using MS medium as the basic medium, adding 3.5mg / L 6-benzylaminopurine, 1.2mg / L α-naphthaleneacetic acid, 0.5mg / L polyvinylpyrrolidone, 30g / L sucrose, and 6.5g / L agar Bud differentiation induction medium with a pH value of 5.8-6.0,

[0051] Use 1 / 2 MS medium as the basic medium, add 3-indolebutyric acid 0.02mg / L, sucrose 30g / L, and agar 6.5g / L to prepare a rooting medium with a pH value of 5.8-6.0;

[0052] 2) Collection of konjac explants: collecting healthy, disease-free and uninjured 2 to 3-year-old konjac bulbs as explants;

[0053] 3) disinfection of ko...

Embodiment 2

[0061] A kind of konjac efficient rapid tissue culture propagation method, the method comprises the following steps:

[0062] 1) Preparation of medium: MS medium was used as basic medium, 0.6mg / L of 6-benzylaminopurine, 0.6mg / L of α-naphthaleneacetic acid, 0.5mg / L of polyvinylpyrrolidone, 30g / L of sucrose, agar After 6.5g / L, a callus induction medium with a pH value of 5.8-6.0 was prepared,

[0063] Prepared by using MS medium as the basic medium, adding 1.5mg / L of 6-benzylaminopurine, 0.5mg / L of α-naphthaleneacetic acid, 0.5mg / L of polyvinylpyrrolidone, 30g / L of sucrose, and 6.5g / L of agar Bud differentiation induction medium with a pH value of 5.8-6.0,

[0064] Use 1 / 2 MS medium as the basic medium, add 3-indolebutyric acid 0.02mg / L, sucrose 30g / L, and agar 6.5g / L to prepare a rooting medium with a pH value of 5.8-6.0;

[0065] 2) Collection of konjac explants: collecting healthy, disease-free and uninjured 2 to 3-year-old konjac bulbs as explants;

[0066] 3) disinfectio...

Embodiment 3

[0074] A kind of konjac efficient rapid tissue culture propagation method, the method comprises the following steps:

[0075] 1) Preparation of medium: MS medium was used as basic medium, 2.0mg / L of 6-benzylaminopurine, 2.0mg / L of α-naphthaleneacetic acid, 0.5mg / L of polyvinylpyrrolidone, 30g / L of sucrose, agar After 6.5g / L, a callus induction medium with a pH value of 5.8-6.0 was prepared,

[0076] Prepared by using MS medium as the basic medium, adding 1.0mg / L of 6-benzylaminopurine, 0.1mg / L of α-naphthaleneacetic acid, 0.5mg / L of polyvinylpyrrolidone, 30g / L of sucrose, and 6.5g / L of agar Bud differentiation induction medium with a pH value of 5.8-6.0,

[0077] Use 1 / 2 MS medium as the basic medium, add 3-indolebutyric acid 0.02mg / L, sucrose 30g / L, and agar 6.5g / L to prepare a rooting medium with a pH value of 5.8-6.0;

[0078] 2) Collection of konjac explants: collecting healthy, disease-free and uninjured 2 to 3-year-old konjac bulbs as explants;

[0079]3) disinfection...

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Abstract

A high-efficiency and rapid tissue culture breeding method of konjac is as below: conducting isolated culture on konjac bulbs to induce callus, adventitious buds and adventitious roots; forming a large number of konjac tissue culture seedlings in a short time; then transplanting tissue culture seedlings in a seedbed; supplementing the seedlings with necessary chemicals and fertilizers; and obtaining a konjac breeder's stock after normal lodging. The high-efficiency and rapid tissue culture breeding method of the konjac provided by the invention overcomes the shortcomings of a low breeding coefficient of traditional konjac breeding, high cost of the whole factory rapid breeding, and failure to adapt to the market; the breeding method provided by the invention has high breeding coefficient,large breeding amount, first generation pollution rate lower than 10%, average induction rate higher than 90%, and a concentrated seedling cycle shortened to 3 and a half months. The seedlings can beproduced on an annual basis, the breeder's stock is produced under natural conditions from March to July every year, and under artificial greenhouse conditions at other times. The breeder's stock hasan average weight of 10 grams or more, greatly reduces the production cost and greatly improves the production efficiency.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a method for artificially rapid propagation of konjac seedlings using plant tissue in vitro culture technology and its high-efficiency and rapid konjac for large-scale production of konjac original seeds under natural conditions (or artificial greenhouse conditions). Tissue culture propagation method. Background technique [0002] Konjac is a perennial herb of the family Araceae. Konjac has been cultivated in my country for more than two thousand years. Konjac is a special economic crop containing a large amount of glucomannan in nature. It has high economic value and great development potential. It is widely used in food, medicine, textile, daily chemical, printing and dyeing, papermaking, construction, petroleum, environmental protection and other fields. Known as "health food" and "industrial monosodium glutamate". [0003] In recent years, the konjac market at home and abroad ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘琳琳
Owner 刘琳琳
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