Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer

Abstract

The invention discloses a tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer. The method comprises the following steps: selecting Haworthia cooperivar. pilfera M. B. Bayer leaves in a good growing state as explants, after disinfecting the explants, inoculating the disinfected explants to clustered shoot inducting medium for inducing clustered shoots, then placing the clustered shoots into a rooting medium for taking roots, or after subculturing of the clustered shoots, placing the clustered shoots into the rooting medium for taking roots, and then carrying out seedling adaptation and plant transplanting on intact plantlets after root growing. According to the method, Haworthia cooperivar. pilfera M. B. Bayer seedlings can be obtained by tissue culturing to Haworthia cooperivar. pilfera M. B. Bayer leaves, the material used in the method is easy to obtain, the disinfecting is convenient, the survival rate is high, the production period is short, the propagation coefficient and the propagation speed of the Haworthia cooperivar. pilfera M. B. Bayer are improved, the strain advantages of the Haworthia cooperivar. pilfera M. B. Bayer can be well kept, a great amount of excellent Haworthia cooperivar. pilfera M. B. Bayer seedlings suitable for transplanting can be propagated quickly, the production demands are met, and the economic benefits of planters are improved.

Description

technical field

[0001] The invention relates to a rapid propagation method of plant tissue culture. Background technique

[0002] Yulu (Haworthia cooperivar.pilfera M.B.Bayer) is a soft-leaved variety of succulents in the genus Twelve Volumes of the Agaveaceae (also known as Sun Orchidaceae, Aloe family or Afuhuaceae), native to South Africa. , can be cultivated in many places in the world. The plant is solitary at first, and then gradually grows in groups. The fleshy leaves are arranged in a rosette shape, with convex sides; the tips of the leaves are transparent or translucent, called "windows", with longitudinal dark lines on the surface and small whiskers at the top. , green leaves; loose raceme, small white flowers, with green vertical stripes. Jade dew is small and exquisite, with crystal clear leaves, carved like jade, peculiar and beautiful, like a living handicraft, very cute, decorated with small potted plants on desks, desks, window sills, etc., fresh and elegan...

Examples

Embodiment 1

[0024] 1. Selection and disinfection of explants

[0025] Select Yulu leaves, wash the soil on the surface of Yulu leaves with water, rinse with running water for 5 hours, and dry the water with filter paper. Soak the leaves of Yulu with 0.1% mercuric chloride on the ultra-clean workbench for 1 minute, then wash them with sterile water for 3 to 5 times, each time for 3 to 5 minutes; Soak in 5% sodium hypochlorite aqueous solution for 5 minutes, then wash with sterile water for 4 to 6 times, each time for 5 to 7 minutes, to obtain sterile explants.

[0026] 2. Induction of clustered buds

[0027] Cut the sterile explants into slices of about 0.5 cm, inoculate them in the culture medium for inducing clustered buds in tissue culture bottles, and inoculate 4 to 6 pieces in each bottle. Hours / day, under the environment of 50%~60% of relative humidity, cultivate for 14 days, obtain clustered buds. Among them, the medium for inducing clustered shoots is based on MS medium as the b...

Embodiment 2

[0033] 1. Selection and disinfection of explants

[0034] Select Yulu leaves, wash the soil on the surface of Yulu leaves with water, rinse with running water for 5 hours, and dry the water with filter paper. Soak the leaves of Yulu with 0.1% mercuric chloride on the ultra-clean workbench for 1 minute, then wash them with sterile water for 3 to 5 times, each time for 3 to 5 minutes; Soak in 5% sodium hypochlorite aqueous solution for 5 minutes, then wash with sterile water for 4 to 6 times, each time for 5 to 7 minutes, to obtain sterile explants.

[0035] 2. Induction of clustered buds

[0036] Cut the sterile explants into slices of about 0.5 cm, inoculate them in the culture medium for inducing clustered buds in tissue culture bottles, and inoculate 4 to 6 pieces in each bottle. Hours / day, under the environment of 50%~60% of relative humidity, cultivate for 14 days, obtain clustered buds. Among them, the medium for inducing clustered shoots is based on MS medium as the b...

technical field

[0001] The invention relates to a rapid propagation method of plant tissue culture. Background technique

[0002] Yulu (Haworthia cooperivar.pilfera M.B.Bayer) is a soft-leaved variety of succulents in the genus Twelve Volumes of the Agaveaceae (also known as Sun Orchidaceae, Aloe family or Afuhuaceae), native to South Africa. , can be cultivated in many places in the world. The plant is solitary at first, and then gradually grows in groups. The fleshy leaves are arranged in a rosette shape, with convex sides; the tips of the leaves are transparent or translucent, called "windows", with longitudinal dark lines on the surface and small whiskers at the top. , green leaves; loose raceme, small white flowers, with green vertical stripes. Jade dew is small and exquisite, with crystal clear leaves, carved like jade, peculiar and beautiful, like a living handicraft, very cute, decorated with small potted plants on desks, desks, window sills, etc., fresh and elegan...

Examples

Embodiment 1

[0024] 1. Selection and disinfection of explants

[0025] Select Yulu leaves, wash the soil on the surface of Yulu leaves with water, rinse with running water for 5 hours, and dry the water with filter paper. Soak the leaves of Yulu with 0.1% mercuric chloride on the ultra-clean workbench for 1 minute, then wash them with sterile water for 3 to 5 times, each time for 3 to 5 minutes; Soak in 5% sodium hypochlorite aqueous solution for 5 minutes, then wash with sterile water for 4 to 6 times, each time for 5 to 7 minutes, to obtain sterile explants.

[0026] 2. Induction of clustered buds

[0027] Cut the sterile explants into slices of about 0.5 cm, inoculate them in the culture medium for inducing clustered buds in tissue culture bottles, and inoculate 4 to 6 pieces in each bottle. Hours / day, under the environment of 50%~60% of relative humidity, cultivate for 14 days, obtain clustered buds. Among them, the medium for inducing clustered shoots is based on MS medium as the b...

Embodiment 2

[0033] 1. Selection and disinfection of explants

[0034] Select Yulu leaves, wash the soil on the surface of Yulu leaves with water, rinse with running water for 5 hours, and dry the water with filter paper. Soak the leaves of Yulu with 0.1% mercuric chloride on the ultra-clean workbench for 1 minute, then wash them with sterile water for 3 to 5 times, each time for 3 to 5 minutes; Soak in 5% sodium hypochlorite aqueous solution for 5 minutes, then wash with sterile water for 4 to 6 times, each time for 5 to 7 minutes, to obtain sterile explants.

[0035] 2. Induction of clustered buds

[0036] Cut the sterile explants into slices of about 0.5 cm, inoculate them in the culture medium for inducing clustered buds in tissue culture bottles, and inoculate 4 to 6 pieces in each bottle. Hours / day, under the environment of 50%~60% of relative humidity, cultivate for 14 days, obtain clustered buds. Among them, the medium for inducing clustered shoots is based on MS medium as the b...

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