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Chrysanthemum one-step tissue culture quick breeding method
ActiveCN102939901AConsistent genetic traitsShort reproductive cyclePlant tissue cultureHorticulture methodsPlantletSeedling
The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.
Owner:SICHUAN COLORLINK CO LTD
Rapid tissue culture method for lycium ruthenicum murr
InactiveCN104719165AReduce stepsImprove tissue culture efficiencyHorticulture methodsPlant tissue cultureBiotechnologyMedicine
The invention discloses a rapid tissue culture method for lycium ruthenicum murr, belonging to the technical field of plant tissue culture. The rapid tissue culture method comprises the following steps: explants sterilization, induction culture, rooting culture, subculture, acclimatization and transplantation. According to the rapid tissue culture method, the rooting culture can be carried out during the subculture, so that the tissue culture is high, the operation is simple and convenient, the period is short, and complete plants can be rapidly acquired; the tissue culture process is not influenced by environmental factors.
Owner:四川天府贝瑞生态农业有限公司
Tilapia fry rearing method
InactiveCN103109758ANeat specificationStable genetic traitsClimate change adaptationPisciculture and aquariaAnimal scienceBroodstock
The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.
Owner:化州市光辉养殖场有限公司
Blueberry tissue culture method and special culture medium thereof
InactiveCN102187813AShort training periodLow costHorticulture methodsPlant tissue cultureAcetic acidSolvent
The invention discloses a blueberry tissue culture method and a special culture medium thereof. The special culture medium is a primary culture medium for blueberry tissue culture and is a solid culture medium which is obtained by adding zeatin, naphthyl acetic acid (NAA), a carbon source and gels into basic culture solution; and in the primary culture medium, the zeatin has the final concentration of 2-5mg / L, the NAA has the final concentration of 0.01-0.12mg / L, and the solvent of the basic culture solution is water and solute of the basic culture solution is shown as Table 1. Experiments prove that: the primary culture medium can greatly shorten the culture period of the tissue culture from three months to two months, the inductivity of sprouts is over 90 percent, and the production capacity of tissue culture each year is over 3 million seedlings. The method has the characteristics of high efficiency and time conservation, the produced tissue culture seedlings are low in cost, the average cost of each seedling is 1.5 yuan, the obtained blueberry tissue culture seedlings have consistent inherited characteristics, and the defect that the conventional reproduction coefficient is low is overcome.
Owner:CHINA AGRI UNIV
Fast reproduction of European wild black cherry tissue
InactiveCN101027975AGrow vigorouslyLeaf color dark greenCultivating equipmentsPlant tissue cultureVitrificationAxillary bud
A tissue culture method for fast reproduction of wild European cherry includes such steps as choosing explant, sterilizing it, removing two brocon ends, inoculating it in the inductive culture medium, inductive culture to induce terminal bud and axillary bud, differentiating rosette buds, cutting the rosette buds to obtain single bud, reproductive culture in the reproducing culture medium, rooting culture in the culture medium A and then in culture medium B to obtain tissue cultured seedlings, naturalizing, and transplanting them in the matrix prepared from peat, pearlite and vermiculite.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Acer palmatum Sangokaku tissue culture propagation process
ActiveCN103477983AReduce manufacturing costConsistent genetic traitsHorticulture methodsPlant tissue cultureAxillary budCulture mediums
The invention discloses an acer palmatum Sangokaku tissue culture propagation process which comprises the following steps: S1, explant sterilization; S2, inoculation and propagation, namely inoculating a sterilized stem section with a bud to a culture medium for growth, culturing for 2-3 weeks, and inducing the germination of an axillary bud; S3, enrichment subculture, namely shearing the germinated axillary bud, inoculating on a subculture medium, culturing for 3-5 weeks, and inducing the generation of caespitose buds; S4 rooting culture, namely cutting adventitious buds positioned on the caespitose buds, inoculating on a rooting culture medium, culturing for 2-3 weeks, and inducing the generation of a root. The acer palmatum Sangokaku tissue culture propagation process disclosed by the invention can be used for building a Sangokaku tissue culture propagation system, and realizing the breakthrough of the Sangokaku tissue culture propagation system, is low in production cost, high in propagation coefficient, short in propagation period, an effective way for the fast propagation of acer palmatum Sangokaku, and capable of preventing the virus accumulation, and achieving the easiness for domesticated seedling survival, and by using the process, the issue culture seedlings with consistent inheritable characters and the sterile acer palmatum Sangokaku seedlings can be relatively easily obtained.
Owner:SICHUAN COLORLINK CO LTD
Tea tree cutting seedling cultivation method
InactiveCN107736144AShorten the breeding cycleQuality improvementCultivating equipmentsVegetative propogationEcological environmentShoot
The invention provides a tea tree cutting seedling cultivation method. The method comprises the steps of firstly, selecting a cutting, wherein two-thirds to third-fourths lignified branch of a new shoot with a shoot length of more than 25cm and a stem diameter of 3-5mm is chosen as the cutting; the whole cutting is soaked or quickly dipped in a rooting agent solution, and cutting is conducted; secondly, a seedbed is prepared, wherein peat soils, vermiculites and perlites are used as substrates and placed in a potted tray with 50-60 holes; the substrates are watered thoroughly the day before the cutting; thirdly, cutting is conducted, wherein the cutting is obliquely inserted into the substrates at an angle of 45-50 degrees; fourthly, a seedling stage is managed; fifthly, field planting isconducted, wherein the seedling is planted in a field when the seedling reaches a height of 30-40cm, a conventional field management is conducted. Light seedling substrates are utilized in the method,and the problems of having long cutting seedling cultivation periods, being constrained by natural environment and destroying ecological environment of traditional short cuttings of tea trees are solved.
Owner:SOUTH CHINA AGRI UNIV
Simple tissue culture propagation technology for acerpalmatumseiryu
ActiveCN103493729AAchieve a breakthroughSimple Tissue Culture Reproduction TechniquePlant tissue cultureHorticulture methodsBiotechnologyMedicine
A disclosed simple tissue culture propagation technology for acerpalmatumseiryu comprises the following steps: S1, collecting disease-free acerpalmatumseiryu branches which are born in the same year, removing excess leaves, cutting into stem segments with buds, and disinfecting; S2, inoculating the disinfected stem segments with buds to a culture medium for growth, inducing axillary buds to germinate; and S3, performing rooting culture, cutting directly induced axillalry buds, inoculating to a rooting culture medium to induce root generation. The beneficial effects of the technology comprise that: the tissue culture propagation system for seiryu is estabilished, the seiryupropagation system is broken through, and the simple tissue culture propagation technology is obtained. Tissue culture plants can be rapidly obtained without a callus stage by using the technology, and can be rapidly transplanted and survive after bred; the production cost is low, the obtained tissue culture plants have consistent heredity, then propagation coefficient is high and the propagation period is short; and the technology is an efficient approach for rapid propagation of acerpalmatumseiryu, and virus accumulation is avoided.
Owner:SICHUAN COLORLINK CO LTD
Selenium-rich sugarcane tissue cultivation and planting method
ActiveCN106576476AShort reproductive cycleGrow vigorouslyBiocideExcrement fertilisersNutritive valuesOxygen
The invention discloses a selenium-rich sugarcane tissue cultivation and planting method. The method comprises the following steps that selenium ore powder is applied, planting furrows are dug, selenium-rich fertilizers are added into the planting furrows, stem tip portions of sugarcane plants are subjected to tissue cultivation to obtain plantlets, the plantlets are planted, and selenium-rich leaf fertilizers are sprayed. According to the method, sugarcane seedlings are obtained through tissue cultivation, a T-shaped glass incubator is adopted for cultivation in the tissue cultivation process, meanwhile, sterile air is introduced into the glass incubator, the content of oxygen in the incubator is controlled, and a convection current is formed inside the incubator, so that an environment suitable for growth of tissues is provided, original excellent properties of the sugarcane seedlings are maintained, and the germination rate is high. By carrying out scientific farming management on sugarcane, applying the selenium ore powder and the selenium-rich fertilizers to a planting field during soil preparation and applying the selenium-rich leaf fertilizers during planting, the harvested sugarcane is excellent in property, high in yield and rich in selenium element, and has extremely high nutritive value and economic value.
Owner:宁波远志立方能源科技有限公司
Tissue-culturing quick propagation method of wild rhizoma panacis japonici
InactiveCN1883259AFast growthSolve insufficient resourcesHorticulture methodsPlant tissue cultureEmbryoSkin callus
The invention relates to a method for tissue culture and rapid reproduction of wild P.japonicus, comprising (1)selection and sterilization of explants: selecting stems and leaves of wild P.japonicus, surfacely sterilizing same, as explants for tissue culture; (2)callus culture; (3)callus proliferation to form embryogenic callus; (4)somatic embryos differentiation: somatic embryos differentiate from the surface of embryogenic callus; (5)somatic embryo proliferation to differentiate somatic embryos further; (6)plants regeneration culture to form full plants with roots, stems and leaves; (7)strong seedlings culture; (8)plants transplantation. The invention is provided with high frequency of regeneration plants, fast speed of proloferation, and no shortcoming of easy variation of offspring reproduced by normal seeds.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for cutting and raising seedlings of tutcheria hexalocularia employing container
InactiveCN103598005ASolve reproductive problemsConsistent genetic traitsHorticultureOrnamental treeLandscaping
The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.
Owner:GUANGXI FORESTRY RES INST
Method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds
ActiveCN104488715AConsistent genetic traitsSimplify tissue culture proceduresPlant tissue cultureHorticulture methodsGermplasmBud
The invention discloses a method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds. The method comprises the following specific steps: (1) preparation of a culture medium including a basic culture medium and components of the culture medium at all stages of tissue culture; (2) selection and disinfection of explants; (3) induction and multiplication of bulbs; (4) rooting of strong seedlings; and (5) transplantation. The method disclosed by the invention has the beneficial effects that the direct induction and the plant regeneration of the bulbs are carried out on flower buds of the wide-leaf albuca namaquensis by utilizing a plant-tissue culture technology, and the procedure of tissue-culture operation is simplified, so that a great quantity of high-quality strong seedlings with consistent genetic characters can be obtained in short time, simultaneously, the defects of female-parent scarcity and slowness of conventional multiplication methods are overcome, and the method has the positive significance for both protecting germplasm resources of the albuca namaquensis and industrializing seedling production.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Tissue cultivation quick breeding method of kangarno paws
InactiveCN101112174AFast growthPrevent browningCultivating equipmentsHorticulture methodsAnigozanthosPhacus
The invention relates to a tissue culture and rapid propagation method for anigozanthos and the method is divided into the following steps: (1) preparation of culture medium, including various components of basic culture medium and culture medium in each stage of the tissue culture; (2) selection of explants; (3) pretreatment of explants; (4) disinfection treatment of explants; (5) induction cultivation which is to introduce the induction culture medium for induction cultivation to induce seedling; (6) multiplication culture, the seedling is transferred to multiplication culture medium for multiplication culture of multiple bud; (7) seedling strengthening culture; (8) rooting culture, the multiple bud is cut into single plant which is to be planted in rooting culture medium; (9) cultivation and transplant of tissue culture seedling. The invention has the beneficial effects that by adopting induction culture medium, multiplication culture medium, seedling strengthening culture medium and rooting culture medium and rapid propagation method for anigozanthos, the induction rate of bud is above 90 percent, rooting rate is of 100% and the survival rate of transplanted seedling is above 90 percent, and effectively prevents browning of explants and tissue culture seedling and promotes the differentiation and growth of tissue culture seedling.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for regenerating camphor variety yongjin (Chinese character) plant
InactiveCN103782906AVigorousConsistent genetic traitsHorticulture methodsPlant tissue cultureAxillary budCataphyll
The invention discloses a method for regenerating a camphor variety yongjin (Chinese character) plant. The method comprises the following steps: (1) collecting a new twig of camphor variety 'yongjin', which grows in current year, removing leaves after the new twig is taken back to a laboratory; cutting into stems with 1-2 auxiliary buds, asepticizing the stems, then inoculating stems into MS culture medium containing 6-benzyl amino adenine and indolebutyric acid to induce formation of the auxiliary buds; (2) inoculating the 'yongjin' auxiliary buds which grow well into the MS culture medium containing 6-benzyl amino adenine and indolebutyric acid together with the stems, so as to carry out subculture and multiplication cultivation; (3) cutting proliferated cluster buds which are small, and grow well, with the mean length being about 2.0cm by using a dissecting knife, and then inoculating the cut cluster buds into 1 / 2 MS culture medium containing indolebutyric acid so as to induce formation of adventitious roots. By adopting the method disclosed by the invention, the phenomena of difficult auxiliary buds induction and growth, black death of subcultured and proliferated new leaves, dropping, and weak growth are overcome, and an asepsis material is sub-cultured for a long period of time. Thus, great proliferation is achieved, the induced cluster buds are vigorous in growth, and the inheritable characters of the induced cluster buds are same as those of a female parent.
Owner:NINGBO CITY COLLEGE OF VOCATIONAL TECH +1
Method for aseptically seeding ebony and establishing leaf-cutting rapid propagation system
InactiveCN105145354AConsistent genetic traitsIncrease the multiplication factorHorticulture methodsPlant tissue cultureBudCell budding
The invention relates to a method for aseptically seeding ebony and establishing a leaf-cutting rapid propagation system. The method comprises the following steps: 1, preparing a culture medium, wherein the culture medium comprises a basic culture medium and components for tissue culturing the culture medium at various stages and specifically includes: (1) a basic culture medium: an MS culture medium; (2) a propagation culture medium; (3) a seedling strengthening and rooting culture medium; 2, sterilizing seeds and culturing the seeds; 3, carrying out the leaf-cutting propagation culture: inoculating a basic part of a mature leaf of a plant in the step 2 onto the propagation culture medium; 4, carrying out the rooting and seedling strengthening culture. The method has the beneficial effects that a plant tissue culture technology is used for seeding and carrying out the leaf-cutting rapid propagation of the ebony, and the propagation coefficient is extremely high; moreover, a greater advantage can be achieved compared with the traditional way of propagating buds by using buds, a great amount of high-quality seedlings consistent in inheritable character can be obtained in a short time, the weakness that the conventional propagation way is slow can be overcome, and positive significance on the mass production and preservation of germplasm resources can be realized.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Tissue culture method of begonia
ActiveCN108064689AConsistent genetic traitsHigh differentiation rateHorticulture methodsPlant tissue cultureBudCell budding
The invention discloses a tissue culture method of begonia. The tissue culture method comprises the following steps: pretreatment: taking young leaves of the begonia for cutting, sterilizing and cleaning to obtain a sterilized explant; primary culture: inoculating the sterilized explant into a first culture medium for carrying out the primary culture to obtain primary cluster buds; induction culture: inoculating the primary cluster buds into a second culture medium for carrying out induction culture to obtain rooting seedlings; multiplication culture: cutting leaf blades of the rooting seedlings as female parent, then inoculating the leaf blades into the first culture medium for inducing culture to obtain subcultured cluster buds; inoculating the subcultured cluster buds into a second culture medium for culturing to obtain strong seedlings; acclimatization and transplant: carrying out acclimatization on the strong seedlings and transplanting to obtain domesticated seedlings of the begonia. The tissue culture method has the advantages of capability of improving the survival rate of the begonia hardened seedlings, short culture period, high propagation coefficient, simple tissue culture flow and no restriction from season.
Owner:四川立德种苗科技有限公司
Tissue culture and rapid propagation method of sugarcane
InactiveCN104106467AShort reproductive cycleGrow vigorouslyPlant tissue cultureHorticulture methodsAxillary budSeedling
The invention discloses a tissue culture and rapid propagation method of sugarcane, and relates to the technical field of plant tissue culture. The method comprises the following steps of selecting sugarcane axillary buds; washing the sugarcane axillary buds clean with clear water; sterilizing; inoculating the sugarcane axillary buds to a bud-inducing culture medium; regulating light and temperature; culturing until new buds are grown; transferring the new buds to a seedling culture medium; regulating the light and temperature to obtain seedlings; transferring the seedlings to a rooting culture medium; regulating the light and temperature; culturing to obtain sugarcane tissue cultured seedlings; carrying out seedling-hardening culture; and transplanting the hardened seedlings to an open field for cultivation. The tissue culture and rapid propagation method shortens a propagation period of the sugarcane.
Owner:柳州市汉森机械制造有限公司
Method for carrying out cornus walteri tissue culture subculture propagation by using lanthanum nitrate
ActiveCN112715367AImprove the effect of subculture proliferationReduce manufacturing costAgriculture gas emission reductionPlant tissue cultureBiotechnologyAxillary bud
The invention belongs to the technical field of forest tree tissue culture propagation, and provides a method for performing cornus walteri tissue culture subculture propagation by using lanthanum nitrate. The methodaims to solve the problems of low propagation rate, poor propagation quality and the like of a current cornus walteri tissue culture system. According to the method, MS is adopted as a basic culture medium, tender stems of cornus walteri are taken as explants, axillary bud induction is carried out, then axillary buds are transferred to a proliferation culture medium added with 0.2 mg.L<-1> lanthanum nitrate for subculture proliferation culture, a large number of axillary buds and adventitious buds are generated through induction, and the proliferation rate and the proliferation coefficient reach 91.5% and 18.1 respectively. According to the method, a large number of subculture multiplication materials can be obtained in a short time, the cornus walteri tissue culture subculture multiplication effect is remarkably improved, the subculture bud propagation period is shortened, and therefore the production cost in industrialized seedling raising of cornus walteri tissue culture is reduced.
Owner:山西省林业和草原科学研究院
Pulsatilla tongkangensis somatic embryogenesis and plant regeneration method
InactiveCN104542280AStable differentiationReproduce fastPlant tissue cultureHorticulture methodsSomatic embryogenesisGermplasm
The invention provides a pulsatilla tongkangensis somatic embryogenesis and plant regeneration method. The method comprises the following steps: using leaves of pulsatilla tongkangensis as explants, using heteroauxin and zeatin to process the explants to induce somatic embryogenesis, firstly forming embryonic calli on an induction medium by the explants, further differentiating the embryonic calli to form somatic embryos with a spherical structure, a heart-shaped structure and a torpedo-shaped structure, transforming the somatic embryos after bud maturity into mature seedlings with root stocks and leaves. With adoption of the plant tissue culture method, the reproduction speed is fast, the inheritable character is identical, and meanwhile according to the method, the plant regeneration is more stable than organ differentiation; the method provides an important technical support for large-scale vegetative propagation of pulsatilla tongkangensis, and is an effective approach for germplasm resource preservation and protection.
Owner:LINYI UNIVERSITY
Culture medium composition adapting for blueberry test tube plantlet proliferation as well as method thereof
InactiveCN101176428BStrong targetingImprove applicabilityHorticulture methodsPlant tissue culturePhacusBud
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for culturing scindapsus aureus through terminal bud
ActiveCN106577282AEfficient reproductionShorten the production cyclePlant tissue cultureHorticulture methodsBudSeedling
The invention discloses a method for culturing scindapsus aureus through a terminal bud. The method includes the following steps that 1, the terminal bud is provided; 2, the terminal bud is subjected to inducing culture to from a callus; 3, the callus is subjected to subculture; 4, the callus after subculture is subjected to inducing culture to form clustered shoots; 5, the clustered shoots are subjected to rooting culture to form a root seedling of scindapsus aureus; 6, the root seedling of scindapsus aureus is cultivated. According to the method, with the terminal bud of scindapsus aureus being an explant material, scindapsus aureus rapidly propagates by the utilization of callus culture, there is no limit from seasons and weather, a offspring of scindapsus aureus can be effectively propagated in quantity, the production period is greatly shortened, the inheritable character of the offspring is highly identical with that of parents, and the effective way is provided for large-scale production of scindapsus aureus.
Owner:东方上彩现代农业有限公司
Method for cutting seedling raising of patchouli
InactiveCN109220267AIncreased number of rootsWell developed root systemGrowth substratesCulture mediaRoot systemPerlite
The invention relates to a method for cutting seedling raising of patchouli. The cutting seedling raising method comprises the following steps of 1) selection and treatment of cuttings; 2) cutting; 3)seedling stage management; 4) transplanting and field planting. A substrate selected in the cutting step is prepared from, by mass, 5-7 parts of peat, 0.5-1 part of vermiculite and 0.5-1 part of perlite, and a special plug tray is selected. The method can solve the problems that the survival rate of patchouli cutting propagation is not high, the root germination is slow and the like.
Owner:广东省中药研究所
Huai-pearl chrysanthemum tissue culture method
InactiveCN105660409AShort reproductive cycleGrow vigorouslyPlant tissue cultureHorticulture methodsMicheliaBud
The invention discloses a Huai-pearl chrysanthemum tissue culture method, relating to the technical field of tissue culture. The michelia alba tissue culture method disclosed by the embodiment comprises the following steps: selecting tender and fresh Huai-pearl chrysanthemum as an explant, disinfecting by using alcohol, inoculating to a bud induction culture medium, a rooting culture medium and a strong seedling culture medium in sequence, and finally culturing by seedling hardening. By adopting all the culture media and the method, the breeding period of the Huai-pearl chrysanthemum can be shortened to a greater degree, obtained seedlings are strong in growth, better in growth vigor, and relatively consistent in inheritable character, and the Huai-pearl chrysanthemumis can be bred without season limitation, and can be popularized broadly.
Owner:邓珂
Fast reproduction of European wild black cherry tissue
InactiveCN100553446CGrow vigorouslyLeaf color dark greenCultivating equipmentsHorticulture methodsAxillary budCerasus maximowiczii
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Chrysanthemum one-step tissue culture quick breeding method
ActiveCN102939901BConsistent genetic traitsShort reproductive cycleHorticulture methodsPlant tissue culturePlantletSeedling
The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.
Owner:SICHUAN COLORLINK CO LTD
Tea tree grafting seedling raising method and application thereof
ActiveCN114009233AEasy accessRealize the grafting of four seasonsGraftingCultivating equipmentsRootstockSeedling
The invention relates to the technical field of tea tree culture, in particular to a tea tree grafting seedling raising method and application thereof, and the method comprises the following steps: (1) preparing rootstocks and selecting proper scions; (2) cutting the rootstock; (3) cutting the scions ; (4) inserting the scions; (5) binding; and (6) managing after grafting. The method solves the problems that the existing bud stock grafting time is relatively fixed, the growth vigor of the grafted tea seedlings is relatively slow, the seedling raising period is long, the tea tree seeds are highly heterozygous, and the characters of the grafted seedlings may be different due to the separation of the characters of the seeds and the stocks.
Owner:ANHUI AGRICULTURAL UNIVERSITY
One-step tissue culture and rapid propagation method of grapes
ActiveCN103190345BGrow vigorouslyConsistent genetic traitsHorticulture methodsPlant tissue cultureVitis viniferaPlantlet
The invention discloses a one-step tissue culture and rapid propagation method of grapes. The method comprises the following steps of: S1, sterilizing explants, namely collecting current immature stems of semi-lignified grapes, chopping the immature stems into stems with buds, washing the stems with buds by running water, and sterilizing the stems with buds on a ultra-clean table by a combined sterilizing mode of alcohol and mercuric chloride; and S2, inoculating and propagating, namely inoculating the sterilized stems with buds on a culture medium to grow, and culturing an inoculated culture material in a culture room with illumination intensity of 3000LX and illumination time of 16h / d and at 24-26 DEG.C, so that after one week, the stems with buds grow into complete plants. The method has the beneficial effects that the one-step rapid propagation of simultaneously propagating and rooting the stems with buds of the grapes is realized, the propagation, strong seedling cultivation and rooting culture can be synchronously performed by only one culture medium, the tissue cultured seedlings can grow healthily, the production cost is low, and the obtained tissue culture seedlings are consistent in heredity, the propagation coefficient is high, the propagation period is short, the operation difficulty is reduced, and the culture efficiency is improved.
Owner:SICHUAN COLORLINK CO LTD
A kind of method for plant regeneration of camphor variety Yongjin
InactiveCN103782906BVigorousConsistent genetic traitsHorticulture methodsPlant tissue cultureAxillary budBud
The invention discloses a method for regenerating a camphor variety yongjin (Chinese character) plant. The method comprises the following steps: (1) collecting a new twig of camphor variety 'yongjin', which grows in current year, removing leaves after the new twig is taken back to a laboratory; cutting into stems with 1-2 auxiliary buds, asepticizing the stems, then inoculating stems into MS culture medium containing 6-benzyl amino adenine and indolebutyric acid to induce formation of the auxiliary buds; (2) inoculating the 'yongjin' auxiliary buds which grow well into the MS culture medium containing 6-benzyl amino adenine and indolebutyric acid together with the stems, so as to carry out subculture and multiplication cultivation; (3) cutting proliferated cluster buds which are small, and grow well, with the mean length being about 2.0cm by using a dissecting knife, and then inoculating the cut cluster buds into 1 / 2 MS culture medium containing indolebutyric acid so as to induce formation of adventitious roots. By adopting the method disclosed by the invention, the phenomena of difficult auxiliary buds induction and growth, black death of subcultured and proliferated new leaves, dropping, and weak growth are overcome, and an asepsis material is sub-cultured for a long period of time. Thus, great proliferation is achieved, the induced cluster buds are vigorous in growth, and the inheritable characters of the induced cluster buds are same as those of a female parent.
Owner:NINGBO CITY COLLEGE OF VOCATIONAL TECH +1
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ActiveCN112616666BConsistent genetic traitsOvercome the disadvantages of slow conventional breeding methodsHorticulture methodsPlant tissue cultureBiotechnologyPlantlet
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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InactiveCN103598005BSolve reproductive problemsConsistent genetic traitsHorticultureOrnamental treeLandscaping
Owner:GUANGXI FORESTRY RES INST
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