44results about How to "Consistent genetic traits" patented technology

The invention discloses a rapid tissue culture method for lycium ruthenicum murr, belonging to the technical field of plant tissue culture. The rapid tissue culture method comprises the following steps: explants sterilization, induction culture, rooting culture, subculture, acclimatization and transplantation. According to the rapid tissue culture method, the rooting culture can be carried out during the subculture, so that the tissue culture is high, the operation is simple and convenient, the period is short, and complete plants can be rapidly acquired; the tissue culture process is not influenced by environmental factors.

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.

A tissue culture method for fast reproduction of wild European cherry includes such steps as choosing explant, sterilizing it, removing two brocon ends, inoculating it in the inductive culture medium, inductive culture to induce terminal bud and axillary bud, differentiating rosette buds, cutting the rosette buds to obtain single bud, reproductive culture in the reproducing culture medium, rooting culture in the culture medium A and then in culture medium B to obtain tissue cultured seedlings, naturalizing, and transplanting them in the matrix prepared from peat, pearlite and vermiculite.

The invention discloses an acer palmatum Sangokaku tissue culture propagation process which comprises the following steps: S1, explant sterilization; S2, inoculation and propagation, namely inoculating a sterilized stem section with a bud to a culture medium for growth, culturing for 2-3 weeks, and inducing the germination of an axillary bud; S3, enrichment subculture, namely shearing the germinated axillary bud, inoculating on a subculture medium, culturing for 3-5 weeks, and inducing the generation of caespitose buds; S4 rooting culture, namely cutting adventitious buds positioned on the caespitose buds, inoculating on a rooting culture medium, culturing for 2-3 weeks, and inducing the generation of a root. The acer palmatum Sangokaku tissue culture propagation process disclosed by the invention can be used for building a Sangokaku tissue culture propagation system, and realizing the breakthrough of the Sangokaku tissue culture propagation system, is low in production cost, high in propagation coefficient, short in propagation period, an effective way for the fast propagation of acer palmatum Sangokaku, and capable of preventing the virus accumulation, and achieving the easiness for domesticated seedling survival, and by using the process, the issue culture seedlings with consistent inheritable characters and the sterile acer palmatum Sangokaku seedlings can be relatively easily obtained.

A disclosed simple tissue culture propagation technology for acerpalmatumseiryu comprises the following steps: S1, collecting disease-free acerpalmatumseiryu branches which are born in the same year, removing excess leaves, cutting into stem segments with buds, and disinfecting; S2, inoculating the disinfected stem segments with buds to a culture medium for growth, inducing axillary buds to germinate; and S3, performing rooting culture, cutting directly induced axillalry buds, inoculating to a rooting culture medium to induce root generation. The beneficial effects of the technology comprise that: the tissue culture propagation system for seiryu is estabilished, the seiryupropagation system is broken through, and the simple tissue culture propagation technology is obtained. Tissue culture plants can be rapidly obtained without a callus stage by using the technology, and can be rapidly transplanted and survive after bred; the production cost is low, the obtained tissue culture plants have consistent heredity, then propagation coefficient is high and the propagation period is short; and the technology is an efficient approach for rapid propagation of acerpalmatumseiryu, and virus accumulation is avoided.

The invention discloses a selenium-rich sugarcane tissue cultivation and planting method. The method comprises the following steps that selenium ore powder is applied, planting furrows are dug, selenium-rich fertilizers are added into the planting furrows, stem tip portions of sugarcane plants are subjected to tissue cultivation to obtain plantlets, the plantlets are planted, and selenium-rich leaf fertilizers are sprayed. According to the method, sugarcane seedlings are obtained through tissue cultivation, a T-shaped glass incubator is adopted for cultivation in the tissue cultivation process, meanwhile, sterile air is introduced into the glass incubator, the content of oxygen in the incubator is controlled, and a convection current is formed inside the incubator, so that an environment suitable for growth of tissues is provided, original excellent properties of the sugarcane seedlings are maintained, and the germination rate is high. By carrying out scientific farming management on sugarcane, applying the selenium ore powder and the selenium-rich fertilizers to a planting field during soil preparation and applying the selenium-rich leaf fertilizers during planting, the harvested sugarcane is excellent in property, high in yield and rich in selenium element, and has extremely high nutritive value and economic value.

The invention discloses a method for cutting and raising seedlings of tutcheria hexalocularia employing a container. An offspring plant container seedling which is kept consistent with an excellent single strain in inheritable characters is obtained in manners of selecting an excellent single strain of the tutcheria hexalocularia as a stock plant by an inventor; selecting a nursery land, and preparing soil; preparing and sterilizing matrix; collecting, cutting and processing cuttings; moisturizing, shading, managing after rooting, and the like. Blooming is earlier than sowing seedling, and the cutting survival rate and the transplanting survival rate are high. The method disclosed by the invention is simple to operate, low in production cost and applicable to vegetative propagation of excellent single strain of the tutcheria hexalocularia; the difficulties of propagating and transplanting of the excellent single strain of the tutcheria hexalocularia can be effectively solved; the method can be applied to massive cultivation of seedlings of rare plants and excellent ornamental tree tutcheria hexalocularia; the difficulty of seedling of the key protected wild plant, namely the tutcheria hexalocularia in Guangxi can be broken through; protection of the rare plant tutcheria hexalocularia resource, and application and promotion thereof in landscaping are facilitated.

The invention discloses a method for carrying out bulb induction and plant regeneration of wide-leaf albuca namaquensis by flower buds. The method comprises the following specific steps: (1) preparation of a culture medium including a basic culture medium and components of the culture medium at all stages of tissue culture; (2) selection and disinfection of explants; (3) induction and multiplication of bulbs; (4) rooting of strong seedlings; and (5) transplantation. The method disclosed by the invention has the beneficial effects that the direct induction and the plant regeneration of the bulbs are carried out on flower buds of the wide-leaf albuca namaquensis by utilizing a plant-tissue culture technology, and the procedure of tissue-culture operation is simplified, so that a great quantity of high-quality strong seedlings with consistent genetic characters can be obtained in short time, simultaneously, the defects of female-parent scarcity and slowness of conventional multiplication methods are overcome, and the method has the positive significance for both protecting germplasm resources of the albuca namaquensis and industrializing seedling production.

The invention relates to a tissue culture and rapid propagation method for anigozanthos and the method is divided into the following steps: (1) preparation of culture medium, including various components of basic culture medium and culture medium in each stage of the tissue culture; (2) selection of explants; (3) pretreatment of explants; (4) disinfection treatment of explants; (5) induction cultivation which is to introduce the induction culture medium for induction cultivation to induce seedling; (6) multiplication culture, the seedling is transferred to multiplication culture medium for multiplication culture of multiple bud; (7) seedling strengthening culture; (8) rooting culture, the multiple bud is cut into single plant which is to be planted in rooting culture medium; (9) cultivation and transplant of tissue culture seedling. The invention has the beneficial effects that by adopting induction culture medium, multiplication culture medium, seedling strengthening culture medium and rooting culture medium and rapid propagation method for anigozanthos, the induction rate of bud is above 90 percent, rooting rate is of 100% and the survival rate of transplanted seedling is above 90 percent, and effectively prevents browning of explants and tissue culture seedling and promotes the differentiation and growth of tissue culture seedling.

The invention relates to a method for aseptically seeding ebony and establishing a leaf-cutting rapid propagation system. The method comprises the following steps: 1, preparing a culture medium, wherein the culture medium comprises a basic culture medium and components for tissue culturing the culture medium at various stages and specifically includes: (1) a basic culture medium: an MS culture medium; (2) a propagation culture medium; (3) a seedling strengthening and rooting culture medium; 2, sterilizing seeds and culturing the seeds; 3, carrying out the leaf-cutting propagation culture: inoculating a basic part of a mature leaf of a plant in the step 2 onto the propagation culture medium; 4, carrying out the rooting and seedling strengthening culture. The method has the beneficial effects that a plant tissue culture technology is used for seeding and carrying out the leaf-cutting rapid propagation of the ebony, and the propagation coefficient is extremely high; moreover, a greater advantage can be achieved compared with the traditional way of propagating buds by using buds, a great amount of high-quality seedlings consistent in inheritable character can be obtained in a short time, the weakness that the conventional propagation way is slow can be overcome, and positive significance on the mass production and preservation of germplasm resources can be realized.

The invention discloses a method for culturing scindapsus aureus through a terminal bud. The method includes the following steps that 1, the terminal bud is provided; 2, the terminal bud is subjected to inducing culture to from a callus; 3, the callus is subjected to subculture; 4, the callus after subculture is subjected to inducing culture to form clustered shoots; 5, the clustered shoots are subjected to rooting culture to form a root seedling of scindapsus aureus; 6, the root seedling of scindapsus aureus is cultivated. According to the method, with the terminal bud of scindapsus aureus being an explant material, scindapsus aureus rapidly propagates by the utilization of callus culture, there is no limit from seasons and weather, a offspring of scindapsus aureus can be effectively propagated in quantity, the production period is greatly shortened, the inheritable character of the offspring is highly identical with that of parents, and the effective way is provided for large-scale production of scindapsus aureus.

The invention relates to a method for cutting seedling raising of patchouli. The cutting seedling raising method comprises the following steps of 1) selection and treatment of cuttings; 2) cutting; 3)seedling stage management; 4) transplanting and field planting. A substrate selected in the cutting step is prepared from, by mass, 5-7 parts of peat, 0.5-1 part of vermiculite and 0.5-1 part of perlite, and a special plug tray is selected. The method can solve the problems that the survival rate of patchouli cutting propagation is not high, the root germination is slow and the like.

The invention discloses a Huai-pearl chrysanthemum tissue culture method, relating to the technical field of tissue culture. The michelia alba tissue culture method disclosed by the embodiment comprises the following steps: selecting tender and fresh Huai-pearl chrysanthemum as an explant, disinfecting by using alcohol, inoculating to a bud induction culture medium, a rooting culture medium and a strong seedling culture medium in sequence, and finally culturing by seedling hardening. By adopting all the culture media and the method, the breeding period of the Huai-pearl chrysanthemum can be shortened to a greater degree, obtained seedlings are strong in growth, better in growth vigor, and relatively consistent in inheritable character, and the Huai-pearl chrysanthemumis can be bred without season limitation, and can be popularized broadly.