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Huai-pearl chrysanthemum tissue culture method

A tissue culture and pearl chrysanthemum technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor growth, irregular ramets and slow reproduction speed of the chrysanthemum chrysanthemum, and shorten the breeding cycle. Consistent genetic traits, robust growth effects

Inactive Publication Date: 2016-06-15
邓珂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, pearl chrysanthemum seedlings are mainly propagated by the traditional branching method, which leads to slow propagation speed, and the growth of pearl chrysanthemum is not good, and the ramet seedlings are not neat. Tissue culture can realize the rapid propagation of pearl chrysanthemum, while domestically less research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The tissue culture medium of Pearl Chrysanthemum in this embodiment includes bud induction medium, rooting medium and strong seedling medium: the bud induction medium is based on 2 / 3MS medium, and 50 grams / liter of 6-benzylamino is added Adenine and 25 g / L indole acetic acid; rooting medium is based on MS medium, add 15 g / L gibberellin, 80 g / L glycine and 30 g / L peptone; strong seedling medium is With 1 / 2 MS medium as the basic medium, add 20 g / L naphthalene acetic acid, 10 g / L indole acetic acid, 10 g / L selenomethionine and 20 g / L indole butyric acid.

[0022] The method of tissue culture in this example is as follows: select the young and fresh Huaizhu chrysanthemum as explants, disinfect with 75% alcohol, and then rinse with sterile distilled water for 3 times; inoculate into bud induction medium, and adjust to At room temperature, cultivate for 10 days until new shoots grow; transfer to rooting medium, natural light, adjust the temperature to 22°C, and cultivate for...

Embodiment 2

[0024] In this embodiment, the pearl chrysanthemum tissue culture medium includes bud induction medium, rooting medium and strong seedling medium: the bud induction medium is based on 2 / 3MS medium, and 60 grams / liter of 6-benzylamino is added Adenine and 20 g / L indole acetic acid; rooting medium is based on MS medium, add 20 g / L gibberellin, 100 g / L glycine and 40 g / L peptone; strong seedling medium is Using 1 / 2 MS medium as the basic medium, add 8 g / L naphthalene acetic acid, 5 g / L indole acetic acid, 3 g / L selenocystamine hydrochloride and 10 g / L indole butyric acid.

[0025] The method of tissue culture in this example is as follows: select young and fresh Huaizhu chrysanthemums as explants, disinfect them with 75% alcohol, and rinse them with sterile distilled water for 4 times; At room temperature, cultivate for 8 days until new shoots grow; transfer to rooting medium, natural light, adjust the temperature to 26°C, and cultivate for 14 days until 2 cm roots grow; transfer...

Embodiment 3

[0027] In this embodiment, the pearl chrysanthemum tissue culture medium includes bud induction medium, rooting medium and strong seedling medium: the bud induction medium is based on 2 / 3MS medium, and 70 grams / liter of 6-benzylamino is added Adenine and 10 g / L indole acetic acid; rooting medium is based on MS medium, add 30 g / L gibberellin, 60 g / L and 50 g / L peptone; strong seedling medium is based on 1 / 2 MS medium was the basic medium, supplemented with 15 g / L naphthalene acetic acid, 7.5 g / L indole acetic acid, 7 g / L selenocystamine hydrochloride and 15 g / L indole butyric acid.

[0028] The method of tissue culture in this example is as follows: select young and fresh Pearl Chrysanthemum as explants, disinfect with 75% alcohol, and rinse with sterile distilled water for 3 times; inoculate into bud induction medium, and adjust to At room temperature, cultivate for 6 days until new shoots grow; transfer to rooting medium, natural light, adjust the temperature to 24°C, and cul...

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PUM

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Abstract

The invention discloses a Huai-pearl chrysanthemum tissue culture method, relating to the technical field of tissue culture. The michelia alba tissue culture method disclosed by the embodiment comprises the following steps: selecting tender and fresh Huai-pearl chrysanthemum as an explant, disinfecting by using alcohol, inoculating to a bud induction culture medium, a rooting culture medium and a strong seedling culture medium in sequence, and finally culturing by seedling hardening. By adopting all the culture media and the method, the breeding period of the Huai-pearl chrysanthemum can be shortened to a greater degree, obtained seedlings are strong in growth, better in growth vigor, and relatively consistent in inheritable character, and the Huai-pearl chrysanthemumis can be bred without season limitation, and can be popularized broadly.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of pearl chrysanthemum. Background technique [0002] Huaizhu chrysanthemum is a variety of Huai chrysanthemum cultivated by the Wenxian Agricultural Science Research Institute using the systematic breeding method. It is a perennial herb with purple stems and no edges. It is erect and clustered, 85-110 cm high, with few branches and densely covered. White hairs, long lignified base, alternate single leaves, ovate-lanceolate, gray-green leaves, deep leaf cracks, leaf length 4.9 cm, width 3.3 cm, stalk length 1.6 cm, flower head, terminal or axillary. The flowers are oblate, with a diameter of 2.5 cm. The ligulate flowers on the periphery of the inflorescence are arranged in multiple rounds. The color is initially white and then turns pink, and turns red after frost. The middle is yellow tubular flowers. The flowering period is mid-to-late October...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓珂
Owner 邓珂
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