45 results about "L-Selenomethionine" patented technology

Disclosed is a novel synthetic method for isomeric peptides through an appropriate linkage of L-selenomethionine or Se-Methyl-L-selenocysteine with L-glutamic acid. The novel synthetic method produces isomeric peptides of L-selenomethionine or Se-Methyl-L-selenocysteine that exhibit (i) enhanced water solubility; (ii) enhanced rate of dissolution in water; (iii) enhanced bioavailability; (iv) excellent vascular endothelial growth factor promoting activity; (v) excellent anti-5-alpha-reductase activity; (vi) capabilities to prevent / reduce “hair fall” and promote “hair growth”, thereby maintaining a perfect homeostasis for “hair care”. Cosmeceutical and pharmaceutical compositions comprising the isomeric peptides obtained through an appropriate linkage of L-selenomethionine or Se-Methyl-L-selenocysteine with L-glutamic acid are also disclosed. Other dipeptides with several other amino acids and uses thereof are also disclosed.

The invention discloses a preparation method for selenomethionine, which includes the steps of: (a) performing a reaction to L-methionine and dimethyl carbonate to remove a methylthio group and meanwhile form a ring to generate L-[alpha]-amino-[gamma]-butyrolactone, and adding hydrobromic acid to carry out a reaction to obtain L-[alpha]-amino-[gamma]-butyrolactone hydrobromate; (b) performing a reaction to selenium with hydrazine hydrate to generate sodium diselenide, adding a methylation reagent, dimethyl carbonate, to carry out a reaction to generate dimethyl diselenide, and reducing the dimethyl diselenide with sodium borohydride to obtain sodium methylselenide; and (c) performing a heating reflux reaction to the L-[alpha]-amino-[gamma]-butyrolactone hydrobromate and sodium methyl selenol to obtain L-selenomethionine sodium salt, and regulating the pH of the reaction liquid to 5-6 with acetic acid, and dehydrating the reaction liquid to obtain the target product L-selenomethionine. The preparation method has high product yield and simple synthetic process, is prepared from easy-to-obtain raw materials, has mild reaction conditions and is easy to carry out industrially.

The invention belongs to the field of feeds, and particularly relates to a premix feed for pigs and a production method of the premix feed. The premix feed comprises the following raw materials in parts by weight: 30-45 parts of dandelions, 26-32 parts of common goldenrop, 15-27 parts of honeysuckle flowers, 9-15 parts of costus roots, 12-18 parts of climbing groundsel herbs, 10-26 parts of haws,3-8 parts of fleeceflower roots, 2-9 parts of Chinese prickly ash, 12-24 parts of star aniseeds, 3-9 parts of garlicin, 28-35 parts of chitosan oligosaccharide, 14-20 parts of lysine, 1-3 parts of L-selenomethionine, 16-35 parts of bone meal, 1-5 parts of a microecological preparation and 0.5-2 parts of composite vitamins. The premix feed disclosed by the invention has the beneficial effects thatthe incidence rate of influenza, epidemic diarrhea, paratyphoid and transmissible gastroenteritis of the pigs can be significantly reduced.

The invention discloses a compound interlayer type chelating magnesium calcium tablet easy to absorb. The compound interlayer type chelating magnesium calcium tablet easy to absorb comprises an inner core and an outer later which wraps the inner core. The inner core is prepared from magnesium L-threonate, calcium L-threonate, vitamin D3 and L-selenomethionine. The outer layer is prepared from calcium carbonate which is slightly dissolved in water, magnesium L-threonate and calcium L-threonate are easily dissolved in water, the calcium carbonate which is slightly dissolved in water wraps magnesium L-threonate and calcium L-threonate which are easily dissolved in water, and therefore the compound interlayer type chelating magnesium calcium tablet is convenient to take for use, the outer layer calcium carbonate is decomposed in the stomach through gastric acid, and L-threonate, calcium L-threonate, vitamin D3 and L-selenomethionine in the outer layer calcium carbonate are released. Calcium salt and magnesium salt in the calcium tablet are in the type of chelate and can be absorbed by human bodies more easily.

The invention provides a preparation method for selenomethionine. The selenomethionine comprises L-selenomethionine, D-selenomethionine and DL-selenomethionine. The preparation method for the selenomethionine comprises the following steps: step 1, subjecting selenium powder, an alkali and hydrazine hydrate to a reaction; step 2, adding a bicarbonate; step 3, preparing methyl hydroselenide; and step 4, preparing the selenomethionine. The preparation method for the selenomethionine provided by the invention has the advantages of cheap and easily-available raw materials, mild reaction conditions,simple process steps, high product yield, low production cost, greenness, environmental protection, safety, high efficiency and applicability to industrial production.

A synthesis of and use for L-Se-methylselenocysteine as a nutriceutical is described, based upon the knowledge that L-Se-methylselenocysteine is less toxic than L-selenomethionine towards normal cells. The synthesis proceeds by mixing N-(tert-butoxycarbonyl)-L-serine with a dialkyl diazodicarboxylate and at least one of a trialkylphosphine, triarylphosphine, and phosphite to form a first mixture that includes N-(tert-butoxycarbonyl)-L-serine β-lactone. Methyl selenol or its salt is mixed with the N-(tert-butoxycarbonyl)-L-serine β-lactone to form a second mixture that includes N-(tert-butoxycarbonyl)-Se-methylselenocysteine. The tert-butoxycarbonyl group is removed from the N-(tert-butoxycarbonyl)-Se-methylselenocysteine to form L-Se-methylselenocysteine. This synthesis significantly improves the manufacturability, manufacturing efficiency, and utility of this naturally occurring rare form of organic-selenium. L-Se-methylselenocysteine formed, for example, in this manner may be used as a nutriceutical for supplementation into the diets of humans or animals for various beneficial purposes, such as, for example, to prevent or reduce the risk of developing cancer.

The invention provides an L-selenomethionine preparation method. The L-selenomethionine preparation method comprises the following steps: A), implementing nucleophilic substitution on sodium methyl hydroselenide and 3,6-bi-(2-chloroethyl)-2,5-diketone piperazine to obtain 3,6-bi-(2- methyl selenide ethyl)-2,5-diketone piperazine; B), hydrolyzing the 3,6-bi-(2- methyl selenide ethyl)-2,5-diketone piperazine obtained in the step A) in a hydrochloric acid solution to obtain L-selenomethionine. The 3,6-bi-(2-chloroethyl)-2,5-diketone piperazine is used as a reaction substrate, substitutes, and ishydrolyzed and neutralized with a nucleophilic reagent methyl hydroselenide to obtain the L-selenomethionine, the process is simple, the reaction condition is mild, the product is single, the L-selenomethionine is easy to separate, the yield is high, and the preparation method is suitable of industrial production.

The invention discloses a selenium-enrichment rice cultivation technology. The technology is implemented as follows: a nutrient solution is sprayed at the last stage of rice tillering, wherein leaf surfaces need to be covered uniformly by the solution. The nutrient solution is prepared as follows: (1), raw materials are prepared; to be specific, 100 parts of purified water, 1.5 to 2.5 parts of L-selenomethionine, 2.5 to 3.5 parts of water-soluble compound fertilizer with the NPK proportion of 15 to 15 to 15, and 0.5 to 1.0 part of hydroxyethyl cellulose; (2), after mixing of the raw materials prepared at the step (1), the mixed raw materials are placed into a pressure tank, pressurization is carried out to reach 4kg / cm<2> and the pressure is kept to be uniform for 2 to 3min, and then the pressure returns to a normal pressure within 1 to 3s to obtain serous fluid; liquid bacillus subtilis spawn with maturation is introduced into the serous fluid based on a percent by volume of 5 to 10% at a temperature of 25 DEG C, the mixture is placed into a fermentation cylinder, sterile air is guided into the fermentation cylinder, stirring and fermentation are carried out; and after fermentation completion, the temperature rises to 70 DEG C rapidly and the temperature is kept for 0.5 to 1.0h for inactivation, thereby obtaining the nutrient solution.

The invention discloses a preparation for ensuring safety of paddy planted in lead mild-moderate polluted soil, a preparation method comprises the following steps: adding 1L of water in a reaction vessel, adjusting water temperature to 50-60 DEG C; then respectively adding 20-30g of L-methionine, 120-180g of L-cysteine, 30-45g of L-selenomethionine, 100-120g of potassium pyrophosphate, 100-120g of ammonium sulfate and 12-14g of compound sodium nitrophenolate in order for mixing, cooling the obtained object to room temperature, filtering, disinfecting, and loading to obtain the finished product. The invention also provides a method for ensuring safe output of paddy planted in lead mild-moderate polluted soil by using the preparation. By employing the method, lead content of paddy rice grains in mild lead pollution region is lower than a limit value of lead in rice in a food hygienic standard in our country (the total lead amount does not exceed 0.2mg / kg).

The invention discloses a selenium-enriched rice planting method. The method includes the following steps that a nutrient solution is sprayed in the later tillering stage of rice until the leaf surface is uniformly covered with the nutrient solution, and the nutrient solution is prepared from the following preparing method including the steps that 1, raw materials are prepared, wherein 100 parts of purified water, 1.5-2.5 parts of L-selenomethionine and 0.5-1.5 parts of hydroxyethyl cellulose are prepared; 2, after being mixed, the raw materials in the step 1 are put into a steam bursting tank at the pressure of 4MPa, the materials are heated to 150 DEG C and kept at the temperature for 2 min, then pressure is fast released within 1-3 s to obtain slurry, the slurry is inoculated with bacillus subtilis liquid strain cultured to be mature according to the volume ratio of 5% at the temperature of 25 DEG C, the mixture is put into a fermentation tank, sterile air is introduced into the fermentation tank, stirring is carried out for fermentation at the stirring speed of 250 r / min, the fermentation time is 3 h, the temperature is kept at 35-37 DEG C, and after fermentation is finished, the temperature is rapidly raised to 70 DEG C and kept for 0.5 h to carry out inactivation to obtain the nutrient solution.

The invention discloses a method for cultivating selenium-rich peanuts. The method comprises the following steps of 1, soil preparation and fertilization; 2, seed selecting and sowing; 3, field management and further comprises the step that a cultivation nutrient solution is sprayed to leaves in the peanut blooming and seed growing period, the fruiting period and the later growth and development period. The cultivation nutrient solution is prepared through a method including the following steps that 1, raw materials are prepared, wherein 100 parts of purified water, 0.6-1.0 part of L-selenomethionine, 1.5-2.5 parts of water-soluble compound fertilizer with NPK being 15-15-15 and 0.1-0.3 part of hydroxyethyl cellulose are prepared; 2, after the raw materials in the step 1 are mixed, the mixture is placed in a steam cavity charge container, the pressure is 4 MPa, the mixture is heated to 150 DEG C, the temperature is kept for 2 min, then the pressure is fast released within 1 s to 3 s, slurry is obtained and inoculated with 5%-10% by volume of bacillus subtilis liquid strain cultured to be mature at the temperature of 25 DEG C, the mixture is placed in a fermentation tank, sterile air is introduced into the fermentation tank, stirring fermentation is carried out, after fermentation is completed, the temperature is fast raised to 70 DEG C to be kept for 0.5-1.0 h, inactivation is carried out, and the cultivation nutrient solution is obtained.

The invention provides a laying hen feed additive, which is composed of the following raw materials by weight percentage: 56.4% to 69.3% of Chinese prickly ash seed, 30% to 40% of Schizochytrium Limacinum powder, 0.25% to 1.5% of Haematococcus pluvialis powder, 0.25-1.5% of lipase, and 0.2% to 0.6% of a L-selenomethionine preparation. The obtained product is added to a laying hen feed in a ratio of 2 to 4 wt%, and the materials are mixed uniformly for feeding. The Chinese prickly ash seed, Schizochytrium Limacinum powder, Haematococcus pluvialis powder, lipase and L-selenomethionine are combined to prepare the feed additive of the laying hen, the feed additive can significantly improve the enrichment rate of DHA in the egg and can also significantly improve the egg quality, and reduces thecholesterol content of the egg. The feed additive of the invention has obvious effect, and the lower omega-3 polyunsaturated fatty acid raw material can achieve higher egg DHA enrichment, and the eggproduction rate is not reduced, and the economic benefits can be increased.

The invention discloses nutrient solution for cultivating selenium-rich rice. A method for preparing the nutrient solution includes 1), preparing raw materials including 100 parts of purified water, 1.5-2.5 parts of L-selenomethionine and 0.5-1.0 part of hydroxyethyl celluloses; 2), mixing the raw materials prepared at the step 1) with one another to obtain mixtures, placing the mixtures in a steam explosion tank under the pressure of 4 MPa, heating the mixtures until the temperatures of the mixtures reach 150 DEG C, keeping the temperatures of the mixtures unchanged for 2 min, then quickly releasing the pressure within 1-3 s to obtain serous fluid, inoculating cultured mature bacillus subtilis liquid strains into the serous fluid at the temperature of 25 DEG C according to a volume percent of 5-10%, placing the serous fluid and the bacillus subtilis liquid strains in a fermentation tank, filling the fermentation tank with sterile air, stirring and fermenting the serous fluid and the bacillus subtilis liquid strains to obtain products, quickly increasing the temperatures of the products until the temperatures reach 70 DEG C after the serous fluid and the bacillus subtilis liquid strains are completely fermented, keeping the temperatures unchanged for 0.5-1.0 h and inactivating the products to obtain the nutrient solution.

The invention discloses moso bamboo shoot selenium-rich nutri-plus gel and a preparation method and application thereof, and belongs to the technical field of bamboo shoot nutrient solutions. The nutri-plus gel is prepared from the raw materials of potassium nitrate, calcium superphosphate, dicyandiamide, amino acid fertilizer, ferric chloride, L-selenomethionine, glucose powder, a modified carrageenan solution and xanthan gum; and the modified carrageenan solution is obtained in the mode that carrageenan is subjected to selenylation and then modified by zein. According to the moso bamboo shoot selenium-rich nutri-plus gel and the preparation method and application thereof provided by the invention, the prepared nutri-plus gel is used for the shooting period of moso bamboo shoots, can adhere to soil, is not prone to being washed away by rainwater, provides nutrients for bamboo forests continuously, and is good in selenium-rich effect, the quality of the obtained moso bamboo shoots is high, and female bamboos can grow normally while the high yield of the bamboo shoots is kept.

The invention discloses a Se-rich rice cultivation method. The method comprises following steps: a nutrient solution is sprayed at the late tillering stage of rice until leaf surfaces are uniformly covered with the nutrient solution, and the nutrient solution is prepared with a method comprising steps as follows: 1), raw materials including 100 parts of pure water, 1.5-2.5 parts of L-selenomethionine and 0.5-1.0 part of hydroxyethyl cellulose are prepared; 2), the raw materials in the step 1) are mixed and then are ground into a thick liquid, the thick liquid is obtained, sterilized, cooled to 25 DEG C, and inoculated with 5%-10% by volume of bacillus subtilis liquid strains which are cultured to be mature, then the thick liquid is added to a fermentation cylinder, sterile air is introduced into the fermentation cylinder, the thick liquid is subjected to mixing fermentation, the temperature is rapidly increased to 70 DEG C after fermentation and kept for 0.5-1.0 h, the thick liquid is inactivated, and the nutrient solution is obtained.

The invention discloses a ganoderma lucidum bag-cultivation culture medium capable of increasing the content of ganoderma lucidum polysaccharides and selenium in ganoderma lucidum. The ganoderma lucidum bag-cultivation culture medium is prepared from the following raw materials in parts by weight: 75 parts of sawdust, 20 parts of wheat bran, 2 parts of maize meal, 1 part of lime, 1 part of gypsum and 1 part of sugar, wherein the material / water ratio is adjusted to be 1: (1.3 to 1.5) by adopting a selenium-enriched nutrient solution. The selenium-enriched nutrient solution is prepared by the following preparation methods: (1) carrying out raw material preparation: preparing 100 parts of pure water, 1.0 part of L-selenomethionine and 5 parts of maize meal; and (2) mixing the raw materials obtained in the step (1), putting the mixture in a steam explosion pot with the pressure of 4MPa, performing heating to the temperature of 150 DEG C, carrying out maintaining for 2 minutes, then, carrying out rapid pressure relief in 1 to 3 seconds so as to obtain slurry, inoculating the slurry with well-cultured liquid bacillus subtilis strains according to the volume percent of 10% at the temperature of 25 DEG C, putting the inoculated slurry into a fermentation tank, introducing sterile air into the fermentation tank, carrying out fermentation for 4 hours under stirring at the stirring revolution rate of 250rpm, maintaining the temperature to 35 DEG C to 37 DEG C, rapidly raising the temperature to 70 DEG C after fermentation is completed, and maintaining the temperature for 1.0 hour for inactivation, thereby obtaining the selenium-enriched nutrient solution.

The invention discloses a planting method of selenium-enriched alfalfa, comprising the following steps: cultivating alfalfa according to a conventional method; when the alfalfa grows for one year, spraying an L-selenomethionine solution into soil for 30 days, from 8:00 a.m. to 10:00 a.m. every day, wherein spraying is not performed in rainy days; taking cultivation and management measures of alfalfa during spraying the same as usual during spraying; after spraying, harvesting to obtain the selenium-enriched alfalfa. The invention also discloses application of the selenium-enriched alfalfa to aselenium-enriched alfalfa feed additive and a preparation method, and application of the feed additive in cow feeding. By adopting the method for planting the selenium-enriched alfalfa provided by the invention, organic selenium L-selenomethionine can be effectively enriched in the alfalfa, and the feed additive finally prepared by a combination of leaves, roots and stems of the alfalfa obtainedby the planting can increase the selenium content in milk.

The invention discloses a method of cultivating rice rich in selenium. According to the method, nutrient solution is sprayed at the end of the tillering stage of the rice until the surface of foliage is evenly covered. The nutrient solution is obtained by the following steps: (1) preparation of raw materials: 100 parts of pure water, 1.5 to 2.5 parts of L-selenomethionine, and 0.5 to 1.0 part of hydroxyethyl cellulose; (2) the raw materials prepared in step (1) are mixed and placed in a high-pressure tank, which is pressurized to 4kg / cm2, the pressure is distributed evenly, maintained for 2 to 3 minutes, and then restored to atmospheric pressure in 1 to 3 seconds, and therefore the slurry is obtained. At the temperature of 25 DEG C, by volume percentage of 5 to 10%, liquid strain seeds of maturely-cultivated bacillus subtilis are inoculated into the slurry, which is then placed into a fermentation tank. Sterile air is introduced into the fermentation tank in which the slurry is stirred and fermented. After completion of the fermentation, temperature of the slurry is quickly raised to 70 DEG C and maintained for 0.5 to 1.0 hour for the purpose of inactivation. The nutrient solution is obtained.

The invention discloses a method for extracting, purifying and inspecting selenomethionine in Astragali mushroom and relates to the field of extraction, purification and inspection of amino acid in Astragali mushroom. The method comprises the following steps: taking the Astragali mushroom as a raw material, adding a nitric acid solution of 5 percent with the material-liquid ratio being 1:6, centrifuging, adding Tris-Hcl into the precipitate, performing ultrasonic treatment, performing enzymolysis for 36 hours, centrifuging for 10 minutes, taking supernatant, performing ultrafiltration centrifugal suction filtration to obtain crude extracting liquid, taking n-butyl alcohol, acetic acid and water with the ratio of 7:1:2 as a developing agent, developing, separating and purifying the selenomethionine through thin layer chromatography, taking chromatographic methanol and ultrapure water as a flowing phase, measuring the molecular mass information of a sample by a mass spectrometer, performing infrared detection on a functional group, analyzing the molecule structure, measuring the content by a double-ultraviolet spectrophotometer, measuring the optical rotation and the specific rotation by a polarimeter, and measuring the melting point after the solution is evaporated and crystallized. According to the method, the low-content sensitive amino acid selenomethionine is not liable to damage, the extraction efficiency is high and the sensitivity is high; and the method is an extraction, purification and detection method which is simple to operate and high in efficiency.

The invention belongs to the technical field of production of raw materials of health food and specifically relates to a preparation method for a broccoli bud seedling soilless culture nutrient solution. A broccoli bud seedling rich in sulforaphane is cultured with the soilless culture nutrient solution prepared from the following components: 0-125 mg / L monopotassium phosphate, 0-150 mg / L dipotassium phosphate, 10-200 mg / L zinc sulfate, 1-50 mg / L L-selenomethionine, 1-50 mg / L L-se-methylselenocysteine, 100-1000 mg / L germanite powder, 5-100 mg / L sodium salicylate, 250-2000 mg / L calcium propionate, 900-1100 mg / L calcium nitrate tetrahydrate, 100-250 mg / L ammonium sulfate, 50-260 mg / L potassium sulfate and the balance of water. The pH is adjusted to 5.5-6.5. Before use, supernatant mother liquor is taken and diluted for 100 times with sterile water.

The invention discloses a preparation method for selenomethionine, which includes the steps of: (a) performing a reaction to L-methionine and dimethyl carbonate to remove a methylthio group and meanwhile form a ring to generate L-[alpha]-amino-[gamma]-butyrolactone, and adding hydrobromic acid to carry out a reaction to obtain L-[alpha]-amino-[gamma]-butyrolactone hydrobromate; (b) performing a reaction to selenium with hydrazine hydrate to generate sodium diselenide, adding a methylation reagent, dimethyl carbonate, to carry out a reaction to generate dimethyl diselenide, and reducing the dimethyl diselenide with sodium borohydride to obtain sodium methylselenide; and (c) performing a heating reflux reaction to the L-[alpha]-amino-[gamma]-butyrolactone hydrobromate and sodium methyl selenol to obtain L-selenomethionine sodium salt, and regulating the pH of the reaction liquid to 5-6 with acetic acid, and dehydrating the reaction liquid to obtain the target product L-selenomethionine. The preparation method has high product yield and simple synthetic process, is prepared from easy-to-obtain raw materials, has mild reaction conditions and is easy to carry out industrially.

The invention discloses a preparation for reducing the arsenic content in rice grains. The preparation is prepared from, by weight, 20-25 g of S-methyl-L-cysteine, 20-25 g of L-selenomethionine, 40-50 g of cystathionine, 150-200 g of mannitol chelated calcium, 100-150 g of potassium pyrophosphate, 5-10 g of ethoxy modified trisiloxane and 10-15 g of polyaspartic acid. The invention further discloses a preparing method of the preparation for reducing the arsenic content in the rice grains. An application method of the preparation comprises the steps that the preparation is applied in the following six periods respectively, and the six periods comprise the stage of soil preparation before rice transplanting, one month after transplanting, the first day to the tenth day in rice seedling stage, the first day to the seventh day in rice tillering stage, the first day to the seventh day in rice flowering stage and the first day to the seventh day in rice filling stage.

The invention discloses a preparation method of high-optical-purity D- or L-selenomethionine. The preparation method comprises the following steps: by taking D- or L-methionine as initial raw materials and diethyl sulfate or halogenated alkyl acid or derived esters as alkylation reagents, generating sulfonium salt, desulfurizing and closing ring under acidic conditions, to produce alpha-amino-gama-butyrolactone halate, having addition reaction to alpha-amino-gama-butyrolactone halate with methyl selenol salt and opening ring, acidifying with organic acid, and recrystallizing to obtain D- or L-selenomethionine. The chemical purity of the product is more than or equal to 99%, and the high optical purity is more than or equal to 99%. The preparation method is low-cost and easily available in raw materials, simple in steps and easy to operate, simple in reaction conditions, and suitable for large-scale production.

The invention discloses an adhesive-dispersion type transdermal patch for cervical spondylosis nursing and a preparation method of the transdermal patch. The transdermal patch is prepared from components in parts by weight as follows: 3.6-9 parts of traditional Chinese medicine extract, 0.6-1.2 parts of traditional Chinese medicine nanopowder, 2.3-5 parts of stigmasteryl glucoside, 2.5-4.5 parts of L-selenomethionine, 1.5-3 parts of L-carnitine, 0.2-0.5 parts of meclizine, 1.5-2.3 parts of isopropyl myristate, 1.2-4 parts of stearyl glycyrrhetinate, 2.5-7 parts of stearic acid, 0.2-0.3 parts of a transdermal accelerant, 30-55 parts of adhesive matrixes and 200-280 parts of a dispersing solvent; the traditional Chinese medicine extract is prepared from 0.4-1 part of Chinese starjasmine fruit extract, 0.4-1 part of extract of all-grass of lanceolate blumea, 0.4-1 part of cajuput extract, 0.4-1 part of extract of herba of Allium funckiaefolium Hand.-Mazz., 0.4-1 part of Cissus repens extract, 0.4-1 part of lindley butterflybush root extract, 0.4-1 part of philippine violet herb extract, 0.4-1 part of juniper extract and 0.4-1 part of starntonia chinensis extract; the traditional Chinese medicine nanopowder is prepared from 0.2-0.4 parts of nanopowder of flowers of Chinese redbud, 0.2-0.4 parts of nanopowder of twigs and leaves of creeping psychotria and 0.2-0.4 parts of nanopowder of Bulbophyllum radiatum Lindl.The adhesive-dispersion type transdermal patch has the efficacy of activating the blood, relieving swelling, activating meridians to stop pain and relieving muscle cramp through skin absorption, promotes microcirculation, reduces capillary permeability, eliminates swelling, relieves local stimulation of nerve roots and relives and eliminates pain caused by nerve root type cervical spondylosis.

The invention discloses a cultivation technique of selenium-rich peanut, which comprises the following steps: (1) land preparation and fertilization, (2) seed selection and seeding, (3) field management. The cultivation technique further comprises foliar spraying nutrient solution in blooming and pegging stage, pod forming stage and the later stage of peanut growth and development. The nutrient solution is prepared by the following steps: (1) preparing raw materials: 100 parts of purified water, 0.6-1.0 parts of L-Selenomethionine, and 0.1-0.3 parts of hydroxyethyl cellulose (HEC); (2) blending the raw materials in step (1), which comprises the steps of putting the blended materials into steam exploded container with a pressure maintained at 4 Mpa, heating to 150 DEG C, holding for 2 minutes, releasing the pressure rapidly within 1-3 seconds, obtaining a slurry, introducing 5%-10% percent by volume a maturely-cultured bacillus subtilis liquid species in 25 DEG C, putting the bacillus subtilis liquid species in to a fermentor, feeding sterile air in the fermentor, fermenting after stirring, raising the heat to 70 EDG C, holding the temperature for 0.5-1.0 hours, and inactivating. The nutrient solution is obtained.

The invention discloses spraying solution for planting of selenium-rich rice. A preparation method of the spraying solution includes: 1) material preparation, to be more specific, preparing 100 parts of purified water, 1.5-2.5 parts of L-selenomethionine, 2.5-3.5 parts of a water-soluble compound fertilizer being 15-15-15 in NPK (nitrogen, phosphorus and potassium) and 0.5-1.0 part of hydroxyethyl cellulose; 2) mixing raw materials prepared at the step 1), adding into a steam explosion tank, heating to 150 DEG C at a pressure of 4MPa, keeping for 2min, quickly releasing the pressure in 1-3s to obtain slurry, performing inoculation of a well-cultured liquid bacillus subtilis strain at 25 DEG C according to a volume percent of 5-10%, adding into a fermentation tank, feeding sterile air into the fermentation tank, stirring for fermentation, quickly heating to 70 DEG C after fermentation is finished, keeping 0.5-1.0h, and inactivating to obtain the spraying solution.

The invention discloses a selenium-enriched peanut cultivation nutrient solution. A preparation method of the selenium-enriched peanut cultivation nutrient solution comprises the following steps: (1) preparation of raw materials: preparing 100 parts of purified water, 0.6-1.0 parts of L-selenomethionine and 0.1-0.3 part of hydroxyethyl cellulose; and (2) mixing the raw materials in the step (1), grinding into thick liquid to obtain pulp, sterilizing, cooling to the temperature of 25 DEG C, inoculating matured bacillus subtilis liquid strain in the pulp according to the volume percent of 5-10%, placing in a fermenting tank, feeding sterile air in the fermenting tank, carrying out stirring fermentation, after fermentation is finished, rapidly heating to the temperature of 70 DEG C, maintaining for 0.5-1.0 hour, and inactivating to obtain the final product.

The invention discloses a selenium-enriched peanut culture nutrient solution. A preparation method of the selenium-enriched peanut culture nutrient solution includes the steps of firstly, preparing raw materials, to be more specific, preparing 100 parts of purified water, 0.6-1.0 part of L-selenomethionine and 0.1-0.3 part of hydroxyethyl cellulose; secondly, mixing the raw materials in the first step, placing into a high-pressure tank, pressurizing to 4kg / cm<2>, allowing the pressure to be even, keeping the pressure for 2-3 minutes, restoring to normal pressure in 1-3 seconds to obtain slurry, inoculating a mature bacillus subtilis liquid strain into the slurry at 25 DEG C according to the volume ratio of 5-10%, placing into a fermentation tank, feeding sterile air into the fermentation tank, performing stirring fermentation, rapidly rising the temperature 70 DEG C after the fermentation, keeping the temperature for 0.5-1.0 hour, and deactivating to obtain the selenium-enriched peanut culture nutrient solution.

A dietary supplement and method for supporting weight loss and reducing the waist circumference of an individual, the dietary supplement comprising an extract of Cissus Quadrangularis stem and leaf, an extract of Green Tea leaf and an effective amount of Caffeine Anhydrous. The dietary supplement may further comprise at least one of Chromium Polynicotinate, an extract of Soy Bean Albumin, Pyridoxine Hydrochloride, L-selenomethionine, Cyanocobalmin, Yohimbine Hydrochloride and Folic Acid.