The invention discloses a method for culturing the selenium-rich iron Dendrobium nobile, comprising: by applying the tissue culture, getting the iron Dendrobium nobile seed for sprout inducing in the sterility tube, primal bulb inducing and domesticating, then replanting it to the culture medium for the culture sprout growing, the composition of the culture medium during the sprout growing comprises per 1000 unit culture medium: macronutrient, 10 units; microelement, 1 unit; ferric salt, 5 units; banana, 100 units; fruitone, 2 units; sugar, 25 units; agar, 9 units; modifying the pH to be 5.6; sodium selenious acid with the concentration to be 200-300 ppm; and it comprises the following macronutrients: potassium nitrate, 14.1497g; amino sulfate, 2.3160 g; potassium phosphite, 2.0046 g; addex-magnesium, 0.9245 g; common salt, 0.6655 g; and the following microelements: potassium iodide, 0.8326 g; sodium permanganate, 0.2532 g; blue copperas, 0.0248 g; cobalt chloride, 0.0256 g; manganese sulfate, 22.3212 g; white copperas, 8.6281 g; boracic acid, 6.2308 g. The selenium-rich iron Dendrobium nobile got in this invention employs the iron Dendrobium nobile as the selenium carrier and transfers into the said product by the adsorption through Dendrobium nobile, and the absorption and utilization rate by human is high and is the most safe health food, combining the medical, nutriment, health and food together.