This invention relates to a
Torreya grandis seed polypeptide with
antioxidant and
hyaluronidase inhibitory activities, its preparation, and its application. The method includes the following steps: S1, mixing
Torreya grandis seed
powder and
ethanol for
defatting to obtain a
solid phase; S2, preparing the
solid phase into a solution, acidifying it, then enzymatically hydrolyzing it with
pepsin, inactivating the
enzyme, centrifuging to collect the
liquid phase, and freeze-
drying it to obtain
Torreya grandis seed polypeptide
powder. Compared with the prior art, this invention provides a simple, reasonable, and efficient preparation method for
Torreya grandis seed polypeptide, exhibiting good activity. It also proposes the application of
Torreya grandis seed polypeptide in
antioxidant and
hyaluronidase activity inhibition, expanding its application in the fields of
cosmeceuticals and food.