213 results about "Bacillus acnes" patented technology

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The present invention discloses a broad-spectrum antibacterial peptide, and the amino acid sequence of the antibacterial peptide is GECEGNNRPVKKPQPRKKLPRFKKC-NH2. Based on melittin, the present invention designs antibacterial peptides by predicting based on a deep learning model, and obtains antibacterial peptides with broad-spectrum bacteriostasis, thermal stability and low hemolytic property, which have good inhibitory effects on skin pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes. Peptides predicted as AMP amino acid sequences by the deep learning model antibacterial peptide prediction tool amPEPpy are selected for screening, and by using a biosynthesis technique, they are expressed, extracted and purified in Escherichia coli, reducing the workload and screening period of screening, and increasing the probability of screening out broad-spectrum and thermally stable biosynthetic antibacterial peptides.

The invention belongs to the technical field of skin care products, and particularly relates to a composition for treating acne and a preparation method thereof. The composition comprises the following components: 1-2% of a lactobacillus fermentation product and 5-8% of a plant extract component, the plant extraction component consists of a Pinus densiflora leaf extract, a celery root extract and an aster extract. The mass ratio of the Pinus densiflora leaf extract to the apium graveolens root extract to the aster extract is 1: (1-3): (5-10). Wherein the apium graveolens root extract, the Pinus densiflora leaf extract and the aster extract have a certain synergistic effect in the formula of the composition, so that the composition for treating acne provided by the invention can effectively inhibit the growth of propionibacterium acnes, reduce the occurrence of skin inflammation and effectively treat acne, and has a relatively good market application prospect.

The invention relates to a natural plant composition for treating acne and application thereof. The composition comprises a scutellaria baicalensis extract, a fructus cnidii extract, a herba violae extract, a mangnolia officinalis extract, a Chinese herbaceous peony extract, a radix ophiopogonis extract, a silybum marianum extract and a gastrodia elata extract. The formula is designed according to the traditional Chinese medicine compatibility theory, and the composition formula has a synergistic effect and can effectively inhibit growth of propionibacterium acnes, reduce skin inflammation and generation of erythema and acne marks after acnes, promote fading of the acnes and skin repair and effectively treat the acnes. The composition is stable in formula quality, safe and free of skin irritation, provides a new thought for acne treatment and skin repair, and has a good market application prospect.

The application discloses a bacteriostatic and acne-removing microemulsion containing plant extracts and a preparation method thereof. The bacteriostatic and acne-removing microemulsion contains a microemulsion loaded with quercetin alone, a microemulsion loaded with sasanquasapernin alone, and a microemulsion loaded with quercetin and sasanquasapernin simultaneously. The bacteriostatic and acne-removing microemulsion containing plant extracts prepared by the application not only solves the problems of stickiness of camellia oil and poor solubility of quercetin, but also has a synergistic inhibitory effect on propionibacterium acnes, achieves the same high-efficiency bacteriostatic effect with a lower drug concentration, saves drug cost, reduces drug irritation, and expands the application of quercetin and sasanquasapernin in the cosmetic and pharmaceutical fields due to the rich pharmacological effect of the plant extracts, low side effect and safety. The microemulsion system prepared by the application is clear, transparent, uniform and stable, the preparation process is simple, the required energy is low, the cost is low, and the system is beneficial to commercial production.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention discloses pyridone endoperoxide with antibacterial and antiviral activity and application thereof, and belongs to the fields of organic compound synthesis and medical technology, food engineering and the like. The invention proves that the series of endoperoxides can effectively kill pathogenic bacteria, fungi and viruses, including propionibacterium acnes, escherichia coli, staphylococcus aureus, candida albicans, human papilloma viruses, herpes simplex viruses and the like. The internal peroxide designed by the invention has the capability of treating skin diseases, is expected to be developed into a first-line medicine for treating skin diseases caused by pathogen invasion, and mainly relates to bacterial skin diseases, fungal skin diseases and viral skin diseases caused by pathogen infection. Meanwhile, the series of endoperoxides can be used for treating oral infection, periodontitis, infection of shoulder and other joint operations, and bacterial infection such as pressure sores. In addition, the peroxides in the series can also be used in the fields of food preservation, disinfectant preparation and the like.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide for inhibiting propionibacterium acnes and application of the antibacterial peptide. The antibacterial peptide for inhibiting propionibacterium acnes is derived from marine organism hylophila, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The amino acid sequence is novel, has remarkable antibacterial activity on propionibacterium acnes, has the advantages of low hemolysis toxicity, low cytotoxicity and high safety, and can be used for preparing the antibacterial peptide for inhibiting propionibacterium acnes. Due to the characteristics, the compound has huge application potential in the aspect of treating acnes caused by propionibacterium acnes or removing acnes.

The invention relates to the technical field of skin care products, in particular to essence with repairing and moisturizing functions and a preparation method thereof.The essence is prepared from, by mass, 0.3%-0.7% of active caffeine, 5%-10% of humectants, 0.3%-0.6% of preservatives, 15%-25% of skin activating ingredients, 0.05%-0.5% of bisabolol, 0.2%-0.6% of stabilizers, 2%-4% of potassium azelaic diglycinate, 0.05%-0.3% of vitamin E and the balance water. And the balance of deionized water. The essence has repairing and moisturizing functions, is non-toxic, harmless and non-irritant to skin, and can relieve facial acnes and inhibit growth and reproduction of propionibacterium acnes.

The invention relates to a specific culture medium for detecting propionibacterium acnes in cosmetics and a detection method thereof, and belongs to the technical field of microbiological detection. Every 1000 mL of the specific culture medium contains 5-25 g of a nitrogen source, 5-10 g of a carbon source, 0.02-16 g of an inhibitor, 0.01-0.02 g of a color indicator, 0.1-13 g of a conditioning agent and the balance of a solvent. The inhibitor comprises Tween 80, a tea tree oil extract, cholate, sodium laurate and hydroxyproline. The specific culture medium provided by the invention is a propionibacterium acnes selective culture medium which is free of antibiotics, high in selectivity, sensitive in color development and low in cost. By optimizing the components of the culture medium and adding the selective inhibitor and the color indicator, the growth of other facultative anaerobic bacteria such as lactic acid bacteria can be effectively inhibited, specific culture and color development detection of propionibacterium acnes are carried out, and the accuracy and reliability of detection are improved.

The invention relates to composite cosmetic nanoparticles with an acne removing effect and a preparation method of the composite cosmetic nanoparticles, and belongs to the technical field of functional skin care products and nano entrapment. According to the nano-particles, low-molecular chitosan is used as a main carrier, and natural multifunctional acne-removing components including a white willow bark extract, a tea catechin substance, a pomegranate peel extract and a hovenia dulcis thunb fruit extract are jointly embedded, so that a stable nano-particle structure with the particle size of 50-200 nm is formed; and sodium hyaluronate is used for surface modification so as to enhance skin adhesiveness and permeability. The multi-target synergistic active component can effectively inhibit the growth of propionibacterium acnes, relieve inflammatory response, regulate sebum secretion and enhance the antioxidant repair capacity of skin. The nano-particles prepared by the invention are concentrated in particle size distribution, high in entrapment rate and good in biocompatibility, are suitable for essence, spray, mask type and gel type acne-removing skin care products, and show excellent product transformation potential.

The invention discloses a novel bifidobacterium infantis strain and application of extracellular products of the novel bifidobacterium infantis strain in delaying skin aging and regulating skin micro-ecology, and bifidobacterium infantis is bifidobacterium infantis BSJM24L007 which is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) and has the preservation number of CGMCC No.33258. The invention further discloses a preparation method of the novel bifidobacterium infantis strain and the application of the extracellular products of the novel bifidobacterium infantis strain. The bifidobacterium infantis extracellular product prepared by the invention has an ideal effect of delaying skin aging, enables the skin to be more compact and elastic, reduces the generation of wrinkles and maintains normal metabolism of the skin, has relatively high safety, can effectively reduce the skin infection problem caused by staphylococcus aureus and propionibacterium acnes, and has a good application prospect. Skin infection and inflammation are reduced, skin health is promoted, and skin micro-ecological balance is maintained. The requirements of a modern skin external preparation on functionality and safety are met.

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to a plant composition with oil control and acne removal effects and a preparation method and application thereof. The invention provides an oil-controlling and acne-removing plant composition. The oil-controlling and acne-removing plant composition is prepared from a rheum palmatum extract, a scutellaria baicalensis extract, a radix sophorae flavescentis extract, a safflower extract, a campsis grandiflora extract, a chrysanthemum extract, a cockscomb extract and a rose extract in a specific proportion. Through synergistic compatibility of eight plants, the plant composition can effectively inhibit grease synthesis, inflammatory factor expression and propionibacterium acnes growth, so that good oil control and acne removal effects are achieved.

The invention provides a micro-ecological preservative composition suitable for sensitive skin. The preparation method comprises the following steps: firstly, carrying out reflux extraction on artemisia apiacea through 60% ethanol, then adding lactobacillus plantarum and saccharomyces cerevisiae for fermentation to obtain artemisia apiacea fermentation liquor powder, and then compounding phenoxyethanol and gluconolactone to obtain the micro-ecological preservative composition. Experiments prove that the micro-ecological preservative composition does not excessively inhibit / promote proliferation of propionibacterium acnes, disturbance and damage to resident bacterial colonies of skin are reduced, and the micro-ecological preservative composition has a certain repairing effect on a micro-ecological barrier. Phenoxyethanol and natural preservatives (sweet wormwood herb fermentation liquor powder and gluconolactone) have a certain synergistic preservative effect, so that the dosage of phenoxyethanol can be reduced, and the irritation and scorching risks of high use concentration of phenoxyethanol to sensitive skin are reduced.

A composition comprising Cutibacterium acnes (also known as Propionibacterium acnes or Corynebacterium parvum), C. acnes culture supernatant and / or a bioactive extract or metabolite obtainable from C.

The application relates to a kind of acne-removing compositions and its preparation method, application, cosmetics.The acne-removing composition comprises: coptis chinensis, magnolia officinalis extract and costus root extract, the coptis chinensis and magnolia officinalis extract are extracted from the mixture of coptis chinensis and magnolia officinalis as extraction raw material, and the costus root extract is extracted from costus root as extraction raw material.The acne-removing composition provided in the application can inhibit sebaceous gland excessive secretion of oil and inhibit the growth of propionibacterium acnes, thereby can reduce skin inflammation and infection, and play the effect of preventing and treating acne.

The invention relates to application of chenodeoxycholic acid or pharmaceutically acceptable salt thereof in preparation of a medicine for treating acne. The chenodeoxycholic acid disclosed by the invention can be a natural organic compound or a synthetic organic compound, has relatively strong propionibacterium acnes inhibitory activity and a pharmacodynamic effect of resisting lipid secretion, and is used as an active component to be prepared into a pharmaceutical composition for treating acnes together with a pharmaceutically acceptable carrier.

The invention provides a lactobacillus plantarum 18 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.31277. The lactobacillus plantarum 18 has the advantages that the lactobacillus plantarum 18 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 18 can inhibit growth of propionibacterium acnes, inhibit expression of virulence factors (biological membranes, lipase and the like) of the propionibacterium acnes, relieve inflammation, inhibit autophagy of cells, scavenge free radicals and inhibit activity of tyrosinase, so that the plant lactobacillus 18 has the effects of resisting acnes, dermatitis and oxidation and whitening skin. The plant lactobacillus 18 or the metabolite of the plant lactobacillus 18 can be used for preparing products with the effects or effects.

The present application relates to a kind of plant anti-dandruff composition with oil control and bacteriostasis efficacy and application thereof, belong to the field of cosmetics.The composition includes soapberry fruit extract, camellia seed extract, artemisia annua extract, valerian root extract, phyllanthus emblica fruit extract;The valerian root extract is extracted after being inoculated with mixed inoculant consisting of bacillus licheniformis and lactobacillus bulgaricus and then fermented with valerian root as raw material.The valerian root extract obtained by the fermentation of specific strains and strain ratio mixture is significantly improved in the bacteriostatic effect on malassezia and propionibacterium acnes.Further, the present application selects specific soapberry fruit extract, camellia seed extract, artemisia annua extract, phyllanthus emblica fruit extract and the obtained valerian root extract within a specific proportion range, and the obtained composition has synergistic effect of each component, which can significantly improve its oil control, bacteriostasis and anti-dandruff effect.

This invention provides ozenafloxacin nanocrystalline particles, its preparation method, and its uses. The preparation method includes the following steps: dissolving ozenafloxacin raw material in an acidic aqueous solution, then adding a stabilizer, stirring, and adjusting the pH to 4-6; wherein the stabilizer is a siloxane and / or a cellulose derivative. The preparation method provided by this invention can obtain ozenafloxacin nanocrystalline particles with uniform and stable particle size distribution. Further research by the inventors has found that formulations containing these ozenafloxacin nanocrystalline particles can increase the amount of drug retained in the skin, thereby increasing the local drug concentration, achieving effective killing of Propionibacterium acnes, and preventing the development of drug resistance.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

The application belongs to the technical field of microorganisms and particularly relates to a Bifidobacterium adolescentis beneficial to skin, a derivative of the Bifidobacterium adolescentis and application of the Bifidobacterium adolescentis and the derivative. The derivative of the Bifidobacterium adolescentis has significant antioxidant and anti-inflammatory effects, can inhibit pathogenic bacteria of acne and other common skin infections such as Propionibacterium acnes, Staphylococcus aureus and Malassezia furfur, can eliminate pathogenic bacteria, can inhibit skin lipid accumulation, can avoid follicle blockage, and can improve skin problems in multiple directions, especially acne skin problems.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a rabbit ear acne model for studying acne pathogenesis and drug curative effect. A healthy New Zealand large-ear white rabbit is selected, a testosterone solution is subcutaneously injected to induce endocrine disorder, oleic acid is smeared at an opening of an external auditory canal on the inner side of an ear, dermatobacterium acnes suspension is subcutaneously injected, and whether acne-like lesions such as erythema, papule and comedo appear on the skin of the ear or not is observed. The method is easy and convenient to operate, low in cost, good in repeatability, capable of simulating the pathological process of human acne and suitable for acne pathogenesis research and drug screening.

The present invention discloses a combined drug for treating acne, which contains bletilla stilbene components and patchouli essential oil for simultaneous or separate administration. By combining bletilla stilbene components and patchouli essential oil in a specific ratio, the present invention significantly inhibits Propionibacterium acnes and acne inflammation, thereby playing a role in treating acne. Moreover, the effect is better than that of either bletilla stilbene components or patchouli essential oil used alone, and is more effective than adapalene gel commonly used at present, showing a synergistic effect and having practical popularization and application value.

The invention discloses an oil-controlling, soothing and repairing composition and a preparation method thereof. The oil-controlling, soothing and repairing composition is prepared from a herba portulacae extract, a herba centellae extract, a baropetalum chinense leaf extract and a plankton extract. According to multiple experimental researches, the composition obtained by compounding the extracts has excellent effects of controlling oil, inhibiting activity of propionibacterium acnes, relieving and repairing, is mild and non-irritant, and has a good popularization and application prospect.

The invention relates to an acne-removing composition with a dual synergistic effect as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The plant active ingredient composition comprises an aquilaria sinensis leaf extract and an acanthopanax trifoliatus leaf / stem extract composition, and the weight ratio of the aquilaria sinensis leaf extract to the acanthopanax trifoliatus leaf / stem extract is that the aquilaria sinensis leaf extract to the acanthopanax trifoliatus leaf / stem extract is (1-4): (1-3). The plant active ingredient composition disclosed by the invention has synergistic antibacterial and synergistic antioxidant effects. By resisting inflammation and oxidative damage caused by proliferation of propionibacterium acnes, acnes are repaired from two aspects of bacteriostasis and oxidative damage resistance. The cosmetic prepared from the plant active ingredient composition has the advantages that the acne improvement condition, the face redness improvement condition and the itching relieving condition are obviously improved, and the overall satisfaction degree is high.

The present application relates to the field of microbial technology, and particularly relates to a gene editing method of propionibacterium acnes VB208 and application thereof. Propionibacterium acnes ) on February 20, 2023, and is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.26576. The propionibacterium acnes screened by the present application has the characteristics of skin surface occupation, high conversion efficiency, gene modification potential and efficient expression of exogenous proteins, and can be used as an engineering strain. Furthermore, a genetic operation system suitable for the strain can be established by using the propionibacterium acnes, and the application prospect is wide.

This invention discloses an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in the indicated mass percentages: 22%~28% helichrysum essential oil, 22%~28% sweet basil essential oil, 22%~28% eucalyptol rosemary essential oil, and 22%~28% true lavender essential oil. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientifically formulated compound. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.