10 results about "Antibacterial protein" patented technology

The invention belongs to the technical field of laundry detergents, and particularly relates to an HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease and a preparation process. The HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease comprises the following components in percentage by mass: 12%-18% of modified alkyl glycoside, 4%-6% of an auxiliary surfactant, 1.5%-3% of a lysozyme-antibacterial protease compound, 0.8%-1.5% of double-chain quaternary ammonium salt, 2%-3% of sodium citrate, 1%-2% of glycerol, 1%-1.5% of citric acid, 0.1%-0.2% of a preservative, 0.1%-0.3% of essence and the balance of deionized water. The laundry detergent disclosed by the invention is mild in effect, short in decontamination time and good in decontamination effect, especially has an excellent decontamination effect on protein and sebum, and is especially suitable for decontaminating underwear.

The application discloses a marine bacteriophage antibacterial lysin prediction method and device, medium and equipment, and relates to the fields of biological medicine and computational biology. The method comprises the following steps: constructing a training data set based on global known antibacterial protein sequences; training a protein sequence classification model based on deep learning by using the antibacterial protein data set to obtain a marine antibacterial lysin prediction model. After data standardization processing, the protein sequence of the bacteriophage to be predicted is input into the protein language model, the input sequence is subjected to self-attention modeling by a ProtT5 module, global structure and function related features are extracted, and a first feature vector is obtained; short sequence local dependency features are extracted by a ProtBERT module, and a second feature vector is obtained; the first and second feature vectors are weighted and summed to generate a fusion feature vector; and the fusion feature vector is subjected to probability prediction, and an antibacterial label and a prediction confidence of the marine bacteriophage sequence to be predicted are output.

The invention discloses preparation and application of a proteoglycan colloid material capable of rapidly stopping bleeding and promoting wound healing, and belongs to the field of biological materials. Basic amino acid rich in lysine or arginine is designed and expressed through molecular engineering, and then basic protein and hyaluronic acid with negative electricity are combined through a supramolecular assembly method to prepare a proteoglycan material with antibacterial ability. Then, recombinant mucoprotein rich in tyrosine is designed and expressed, and a DOPA adhesion group is formed through tyrosinase modification, so that the instant adhesion of the tissue adhesion material is enhanced. The amino acid of the basic protein remarkably enhances the interaction of various molecules of proteoglycan, meanwhile, the basic amino acid endows the tissue adhesive with excellent antibacterial ability, and the tissue adhesive provided by the invention has instant tissue adhesion performance, excellent biocompatibility and excellent antibacterial ability and hemostatic performance, and is suitable for being used in the field of medical instruments. The healing of different types of bleeding scenes and irregular wounds is facilitated.

The application discloses a new method for preventing and treating plant verticillium wilt by scopolamine and bacteriostatic protein CkPGIP1. The method adopts scopolamine and / or protein CkPGIP1 as bacteriostatic agents, and is applied to plants. The nucleotide sequence of the protein CkPGIP1 is shown as SEQ ID No. 1, and the amino acid sequence of the protein CkPGIP1 is shown as SEQ ID No. 2. Scopolamine and / or bacteriostatic protein CkPGIP1 can inhibit the growth of verticillium dahliae in culture medium and gossypium hirsutum plants. Especially, the combination of scopolamine and CkPGIP1 bacteriostatic protein has a very significant inhibitory effect on cotton verticillium wilt, and is expected to become a safe, green and efficient bactericide.

Embodiments herein relate to engineered antimicrobial probiotics for the treatment of gastrointestinal tract pathogens. In an embodiment, a composition for treatment of an animal is included. The composition can include a first genetically engineered bacterium comprising a first exogenous polynucleotide. The first exogenous polynucleotide can include a first heterologous promoter and a first polynucleotide that encodes a first antimicrobial protein. The composition can also include a second genetically engineered bacterium comprising a second exogenous polynucleotide. The second exogenous polynucleotide can include a second heterologous promoter and a second polynucleotide that encodes a second antimicrobial protein. The first heterologous promoter can be induced by one set of exogenous environmental conditions found in the gastrointestinal tract of the animal and the second heterologous promoter can be induced by a second set of exogenous environmental conditions found in the gastrointestinal tract of the animal. Other embodiments are also included herein.

The present application provides an antibacterial protein targeting Enterococcus gallinarum and a preparation method and application thereof. Specifically, the present application provides an antibacterial protein targeting Enterococcus gallinarum or a chimera thereof, wherein the antibacterial protein comprises one or more of the following proteins formed by the following amino acid sequences: (a) an amino acid sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 9; (b) a derived sequence obtained by replacing, adding and / or deleting one or more amino acids from the amino acid sequence of (a) and having the same function as (a); and (c) a derived amino acid sequence having an identity of more than 85% or more than 90% with any one of SEQ ID NO: 1 to SEQ ID NO: 9. The antibacterial protein of the present application can efficiently kill Enterococcus gallinarum and has specificity.

The invention discloses a bacteriophage lyase Lys162 and an application of the bacteriophage lyase Lys162 in preparation of an antibacterial product. The bacteriophage lyase has a remarkable splitting effect on gram-negative bacteria and gram-positive bacteria, so that the bacteriophage lyase can be used as a broad-spectrum antibacterial protein. For example, the antibacterial effect on pseudomonas aeruginosa, klebsiella pneumoniae and streptococcus is remarkable. The antibacterial protein has relatively high temperature tolerance and pH tolerance, can replace antibiotics, and is applied to prevention and treatment of bacterial diseases as an external medicine or an internal medicine and sterilization of medical instruments and medical places; and the product can also be used as an additive to be applied to the fields of feed processing, food industry, animal husbandry, beer industry, aquaculture, cosmetic production, fruit preservation and the like.

A freeze-dried formulation includes an antibacterial protein having a killing activity specific to at least one or all of the following genera: Staphylococcus arlettae, Staphylococcus aureus, Staphylococcus auricularis, Staphylococcus carnosus, Staphylococcus caprae, Staphylococcus chromogenes, Staphylococcus cohnii, Staphylococcus delphini, Staphylococcus epidermidis, Staphylococcus equorum, Staphylococcus gallinarum, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus intermedius, Staphylococcus kloosii, Staphylococcus lentus, Staphylococcus lugdunensis, Staphylococcus muscae, Staphylococcus pasteuri, Staphylococcus saprophyticus, Staphylococcus warneri, and Staphylococcus xylosus; a poloxamer; a sugar; and an amino acid.

The invention relates to the technical field of biological medicine, and discloses a targeted delivery nano-carrier antibacterial protein and a preparation method and application thereof, and the antibacterial protein comprises an antibacterial fusion protein and a liposome-polymer hybrid nano-carrier which are subjected to genetic engineering fusion expression, the fusion protein contains an antibacterial peptide structural domain, a bacterial targeting structural domain and a cell-penetrating peptide structural domain, the nano-carrier is composed of a lipid core and a chitosan shell, the particle size is 80-200 nm, the Zeta potential is + 10-+ 35 mV, the MIC of the preparation on drug-resistant bacteria such as MRSA and CRAB is 0.25-2 microgram / mL, the therapeutic index is larger than 128, and the in-vivo and in-vitro stability is excellent.

The application provides an expression system and application of tilapia antibacterial protein Mptx, and belongs to the technical field of recombinant proteins.The tilapia Mptx eukaryotic protein is obtained by the method of gene cloning and eukaryotic expression vector construction, the binding and agglutination effect of Mptx on related pathogenic bacteria of tilapia are detected by ELISA and agglutination experiment, and it is proved that the obtained Mptx has the effects of inhibiting the proliferation of pathogenic bacteria and protecting the organism by the methods of bacteriostatic experiment and survival rate detection.The application proves that the Mptx eukaryotic protein can significantly reduce the death of tilapia caused by streptococcus agalactiae infection, and has important significance in the aquaculture industry.