44 results about "Antibacterial protein" patented technology

The invention belongs to the technical field of biology, and particularly discloses a high-temperature-resistant antibacterial protein Cystatin B sourced from marine fish as well as a preparation method and application of the high-temperature-resistant antibacterial protein Cystatin B. The high-temperature-resistant antibacterial protein Cystatin B is derived from pomfret, and the amino acid sequence of the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO. 1; the nucleotide sequence of the gene for coding the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO.2, and the sequences of primers adopted for cloning the gene are as shown in SEQ ID NO.3-4. The high-temperature-resistant antibacterial protein Cystatin B has a remarkable antibacterial effect on gram-negative bacteria such as escherichia coli, vibrio parahaemolyticus and edwardsiella tarda as well as gram-positive bacteria such as staphylococcus aureus; and after being treated at the temperature of 100 DEG C, the antibacterial agent still can keep relatively high antibacterial activity. The antibacterial protein is simple in preparation method, can replace traditional antibiotics, and can quickly and efficiently prevent and treat various common bacterial diseases in aquaculture.

The invention belongs to the technical field of laundry detergents, and particularly relates to an HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease and a preparation process. The HPV sterilization underwear laundry detergent containing lysozyme-antibacterial protease comprises the following components in percentage by mass: 12%-18% of modified alkyl glycoside, 4%-6% of an auxiliary surfactant, 1.5%-3% of a lysozyme-antibacterial protease compound, 0.8%-1.5% of double-chain quaternary ammonium salt, 2%-3% of sodium citrate, 1%-2% of glycerol, 1%-1.5% of citric acid, 0.1%-0.2% of a preservative, 0.1%-0.3% of essence and the balance of deionized water. The laundry detergent disclosed by the invention is mild in effect, short in decontamination time and good in decontamination effect, especially has an excellent decontamination effect on protein and sebum, and is especially suitable for decontaminating underwear.

The invention discloses application of an antibacterial agent and bacteriophage lyase. The bacteriophage lyase has a remarkable splitting effect on gram-negative bacteria, so that the bacteriophage lyase can be used as a broad-spectrum antibacterial protein. For example, the antibacterial effect on gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa and klebsiella pneumoniae is remarkable. The antibacterial protein has relatively high temperature tolerance and pH tolerance, can replace antibiotics, and is applied to prevention and treatment of bacterial diseases as an external medicine or an internal medicine and sterilization of medical instruments and medical places; and the product can also be used as an additive to be applied to the fields of feed processing, food industry, animal husbandry, beer industry, aquaculture, cosmetic production, fruit preservation and the like.

The present invention provides a polypeptide as defined by one of the following (i) to (iii), and an antibacterial protein, an antibacterial agent, a disinfectant, a pharmaceutical composition, an antibacterial agent composition, and a disinfectant composition each comprising the following polypeptide: (i) a polypeptide comprising the amino acid sequence represented by SEQ ID NO: 1; (ii) a polypeptide comprising an amino acid sequence obtained by deleting, substituting, inserting and / or adding one or more amino acid residues in the amino acid sequence as shown in SEQ ID NO: 1; or (iii) a polypeptide comprising an amino acid sequence having 80% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO: 1.

The application discloses a marine bacteriophage antibacterial lysin prediction method and device, medium and equipment, and relates to the fields of biological medicine and computational biology. The method comprises the following steps: constructing a training data set based on global known antibacterial protein sequences; training a protein sequence classification model based on deep learning by using the antibacterial protein data set to obtain a marine antibacterial lysin prediction model. After data standardization processing, the protein sequence of the bacteriophage to be predicted is input into the protein language model, the input sequence is subjected to self-attention modeling by a ProtT5 module, global structure and function related features are extracted, and a first feature vector is obtained; short sequence local dependency features are extracted by a ProtBERT module, and a second feature vector is obtained; the first and second feature vectors are weighted and summed to generate a fusion feature vector; and the fusion feature vector is subjected to probability prediction, and an antibacterial label and a prediction confidence of the marine bacteriophage sequence to be predicted are output.

The invention is applicable to the technical field of bioengineering, and provides a streptococcus suis bacteriophage antibacterial protein Lys8387 as well as a preparation method and application thereof, and the amino acid sequence of the streptococcus suis bacteriophage antibacterial protein Lys8387 is shown as SEQ ID NO.2. A novel bacteriophage antibacterial protein capable of playing splitting and depolymerizing double activities on streptococcus suis is constructed through a genetic engineering technology, and the lyase is novel in sequence, shows strong bactericidal activity on multi-drug-resistant streptococcus suis, can be independently used or compounded with other substances for use, or can be prepared into an enzyme preparation, and can be used for preparing an antibacterial agent for multiple drug-resistant streptococcus suis. The compound not only has bactericidal activity on streptococcus suis, but also has a degradation effect on capsular polysaccharide of streptococcus suis, K2 type klebsiella pneumoniae and the like, and a thought is provided for preparing a medicine for preventing and treating diseases caused by infection of microorganisms such as streptococcus suis and the like.

A method for preparing a drug for treating bovine cholecystitis belongs to the field of biological veterinary medicine. The present invention utilizes the original ecological pathogens of bovine cholecystitis, and further adds bovine blood, trace elements, and freeze-dried Escherichia coli powder, freeze-dried enterococcus powder, freeze-dried Pseudomonas aeruginosa powder, and lecithin. Weak ultrasound is used in a buffer solution to activate the activity of various pathogens, and in the intelligent cultivation process, the corresponding antimicrobial peptides and antimicrobial proteins are strongly induced in the body of the big-headed golden fly larvae. The antimicrobial peptides and antimicrobial proteins have the characteristics of high biological activity and broad spectrum of action. Clinical treatment results show that the drug prepared by the present invention has good efficacy and high efficiency. The production process of the present invention is easy to industrialize and control, the product consistency is good, energy is saved, and it is suitable for large-scale production.

The invention discloses bacillus velezensis Jcl-10 and application of the bacillus velezensis Jcl-10 in prevention and treatment of vibrio cholerae. The strain Jcl-10 is preserved in the China Center for Type Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC N: M 2024212. The bacillus velezensis Jcl-10 disclosed by the invention is identified as a gram positive bacterium after being subjected to gram staining. The bacterial strain can produce antibacterial peptide substances, antibacterial protein substances, polyketide synthetase substances and the like with bacteriostatic action, and can be well applied to prevention and treatment of aquatic diseases caused by vibrio cholerae. The Jcl-10 is added into the aquatic feed, so that the morbidity of aquaculture animals can be effectively reduced, and the economic yield of the aquaculture animals can be increased. In conclusion, the bacillus velezensis Jcl-10 disclosed by the invention has outstanding performance in the prevention and treatment of vibrio cholerae, and provides a powerful guarantee for continuous and healthy culture of aquatic products.

The present disclosure provides compositions and formulations comprising a mixture of antibacterial proteins, such as bacteriocins for the treatment of Klebsiella infections. The composition and formulations, as disclosed, comprises a mixture of klebicins (proteins or variants thereof) with broader target cell ranges in order to effectively treat Klebsiella infections. The present disclosure provides kits and methods for treating Klebsiella infections.

The present invention relates to nuclease bacteriocin-derived antimicrobial protein complexes, their preparation and use, and related products having antimicrobial surfaces. The complexes comprise a nuclease bacteriocin polypeptide and an immunity polypeptide linked to a bacteria-binding moiety.

Described is an antibacterial protein S2200 which has specific antibacterial activity against Staphylococci including Staphylococcus aureus. More specifically, described is antibacterial protein S2200 specific for Staphylococci including Staphylococcus aureus, which has the ability to specifically lyse Staphylococci including Staphylococcus aureus and has the amino acid sequence represented by SEQ ID NO: 1; and a pharmaceutical composition for the treatment of infections caused by Staphylococci including Staphylococcus aureus, containing as an active ingredient antibacterial protein S2200 specific for Staphylococci including Staphylococcus aureus.

The invention discloses a lactobacillus plantarum bacteriostatic agent and application thereof, and relates to the technical field of microbial engineering. According to the lactobacillus plantarum LP2301 provided by the invention, fermentation liquor of the lactobacillus plantarum LP2301 can be used for effectively inhibiting aquatic pathogenic bacteria streptococcus agalactiae, limiting the growth of the streptococcus agalactiae, reducing the use of antibiotics, ensuring food safety and guaranteeing the health of animals and human beings by secreting metabolites such as bacteriostatic proteins and organic acids. Secondly, the invention provides an application of the lactobacillus plantarum bacteriostatic agent, and the lactobacillus plantarum LP2301 is compounded into the bacteriostatic agent; through multi-target cooperation of the cortex phellodendri extract and the rosmarinic acid, the antibacterial efficiency of single probiotics is remarkably optimized; active ingredients of cortex phellodendri directionally destroy a biological membrane barrier of streptococcus agalactiae and disintegrate an adhesion defense system of pathogenic bacteria; rosmarinic acid blocks a bacterial energy metabolism pathway synchronously, so that pathogens lose proliferative power; and exclusive bacteriocin secreted by the LP2301 accurately breaks down a cell membrane structure, so that fatal killing is realized.

An antibacterial protein against Bacillus with the amino acid sequence as set forth in SEQ ID NO: 1 is disclosed. Also disclosed is a method of preparing an antibacterial protein against Bacillus. The method includes: culturing BL21-pBAD-BAL200 cells, the BL21-pBAD-BAL200 cells including a plasmid that comprising a sequence as set forth in SEQ ID NO: 2; inducing the expression of the antibacterial protein; and purifying the antibacterial protein.

The invention relates to tomato SGT1 protein, application of the tomato SGT1 protein to prevention and treatment of tomato gray mold and a biological preparation for preventing and treating the tomato gray mold, and belongs to the technical field of tomato disease prevention and treatment. In order to solve the problems that the existing plant-source antibacterial protein is difficult to effectively inhibit botrytis cinerea infection and lags in action time, the invention provides application of the tomato SGT1 protein in prevention and treatment of botrytis cinerea, the amino acid sequence of the tomato SGT1 protein is shown as SEQ ID No: 1, and the tomato SGT1 protein can effectively inhibit the growth of botrytis cinerea hyphae, destroy the completeness of conidia of the botrytis cinerea and inhibit the growth of the botrytis cinerea hyphae. The botrytis cinerea can be effectively prevented and controlled, the infection capacity of botrytis cinerea on tomato leaves is remarkably reduced, the method belongs to typical early-stage infection inhibition, the prevention and control efficiency of the botrytis cinerea can be remarkably improved, 'early intervention and multi-target 'precise prevention and control of dead body nutritional pathogenic bacteria are realized, and an important basis is provided for developing a novel biological agent for preventing and controlling the botrytis cinerea.

The invention discloses preparation and application of a proteoglycan colloid material capable of rapidly stopping bleeding and promoting wound healing, and belongs to the field of biological materials. Basic amino acid rich in lysine or arginine is designed and expressed through molecular engineering, and then basic protein and hyaluronic acid with negative electricity are combined through a supramolecular assembly method to prepare a proteoglycan material with antibacterial ability. Then, recombinant mucoprotein rich in tyrosine is designed and expressed, and a DOPA adhesion group is formed through tyrosinase modification, so that the instant adhesion of the tissue adhesion material is enhanced. The amino acid of the basic protein remarkably enhances the interaction of various molecules of proteoglycan, meanwhile, the basic amino acid endows the tissue adhesive with excellent antibacterial ability, and the tissue adhesive provided by the invention has instant tissue adhesion performance, excellent biocompatibility and excellent antibacterial ability and hemostatic performance, and is suitable for being used in the field of medical instruments. The healing of different types of bleeding scenes and irregular wounds is facilitated.

This disclosure describes compositions and methods involved in tumor suppression and inhibiting infection of cells by pathogens. Generally, the compositions include an mRNA cargo that can provide a therapeutic benefit after being introduced into an epithelial cell. In some cases, the mRNA can encode a polypeptide. In some cases, the polypeptide can suppress epithelial cell proliferation. In other embodiments, the polypeptide can be involved in innate immunity. In various embodiments, the polypeptide can include cathelicin antimicrobial protein (CAMP), calprotectin, S100A8, S100A9, a β-defensin, S100A7, secretory leukocyte inhibitor, lipocalin 2, or lysozyme. In some embodiments, the mRNA can include a stabilizing moiety such as, for example, a 5′ cap or a 3′ extension.

Described are antimicrobial proteins, a nucleic acid sequence encoding the said protein, a gene encoding the said protein or comprising the said nucleic acid sequence, a host cell comprising the said nucleic acid sequence or the said gene, a composition comprising the antimicrobial protein and the use of the antimicrobial protein or the composition as antiseptic agent.

The invention provides an expression system and application of tilapia antibacterial protein Mptx, and belongs to the technical field of recombinant protein. The tilapia mossambica Mptx eukaryotic protein is obtained through gene cloning and eukaryotic expression vector construction methods, the combination and agglutination effects of the Mptx on tilapia mossambica related pathogenic bacteria are detected through ELISA and agglutination experiments, and it is proved through antibacterial experiments, survival rate detection and other methods that the obtained Mptx has the effects of inhibiting pathogenic bacteria proliferation and protecting the organism. The invention proves that the Mptx eukaryotic protein can obviously reduce the death of tilapia caused by streptococcus agalactiae infection, and has important significance in the green aquaculture industry of aquatic products.

The present invention relates to an antibacterial protein SSL200 having a specific antibacterial activity against Staphylococci including Staphylococcus aureus. More specifically, the present invention relates to: an antibacterial protein SSL200 specific for Staphylococci including Staphylococcus aureus, the protein being characterized by having the ability to specifically lyse Staphylococci including Staphylococcus aureus and having an amino acid sequence represented by SEQ ID NO: 1; and a composition for treating infections caused by Staphylococci including Staphylococcus aureus, the composition comprising, as an active ingredient, the antibacterial protein SSL200 specific for Staphylococci including Staphylococcus aureus.

The application discloses a new method for preventing and treating plant verticillium wilt by scopolamine and bacteriostatic protein CkPGIP1. The method adopts scopolamine and / or protein CkPGIP1 as bacteriostatic agents, and is applied to plants. The nucleotide sequence of the protein CkPGIP1 is shown as SEQ ID No. 1, and the amino acid sequence of the protein CkPGIP1 is shown as SEQ ID No. 2. Scopolamine and / or bacteriostatic protein CkPGIP1 can inhibit the growth of verticillium dahliae in culture medium and gossypium hirsutum plants. Especially, the combination of scopolamine and CkPGIP1 bacteriostatic protein has a very significant inhibitory effect on cotton verticillium wilt, and is expected to become a safe, green and efficient bactericide.

The invention relates to the technical field of biological pharmacy, in particular to an immunopotentiator containing high-activity microbial protein peptide and a preparation method of the immunopotentiator. The immunopotentiator containing the high-activity microbial protein peptide comprises the following raw materials: beta-glucan, composite immune peptide, a freeze-drying protective agent, a metal organic framework and the balance of a stabilizer. The preparation method comprises the following steps: firstly, constructing an immune activation core skeleton by using beta-glucan, and activating a phosphorylation pathway by using Kluyveromyces marxianus source peptide in the composite immune peptide; the silkworm chrysalis protein peptide is in targeted combination with a receptor compound, so that the phagocytic rate of macrophages is increased; the recombinant lactoferrin peptide destroys a lipid bilayer of a microbial cell membrane through a cationic structural domain, activates a TLR4 pathway to promote lymphocyte differentiation, and ensures that the systematic immune defense efficacy is improved; specific antibacterial protein contained in the Fusarium brachypodium FXFB001 fermentation active protein extract can directly destroy a pathogen cell membrane structure and activate a TLR2 / 4 pathway to enhance innate immune response at the same time.

Embodiments herein relate to engineered antimicrobial probiotics for the treatment of gastrointestinal tract pathogens. In an embodiment, a composition for treatment of an animal is included. The composition can include a first genetically engineered bacterium comprising a first exogenous polynucleotide. The first exogenous polynucleotide can include a first heterologous promoter and a first polynucleotide that encodes a first antimicrobial protein. The composition can also include a second genetically engineered bacterium comprising a second exogenous polynucleotide. The second exogenous polynucleotide can include a second heterologous promoter and a second polynucleotide that encodes a second antimicrobial protein. The first heterologous promoter can be induced by one set of exogenous environmental conditions found in the gastrointestinal tract of the animal and the second heterologous promoter can be induced by a second set of exogenous environmental conditions found in the gastrointestinal tract of the animal. Other embodiments are also included herein.

The present application provides an antibacterial protein targeting Enterococcus gallinarum and a preparation method and application thereof. Specifically, the present application provides an antibacterial protein targeting Enterococcus gallinarum or a chimera thereof, wherein the antibacterial protein comprises one or more of the following proteins formed by the following amino acid sequences: (a) an amino acid sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 9; (b) a derived sequence obtained by replacing, adding and / or deleting one or more amino acids from the amino acid sequence of (a) and having the same function as (a); and (c) a derived amino acid sequence having an identity of more than 85% or more than 90% with any one of SEQ ID NO: 1 to SEQ ID NO: 9. The antibacterial protein of the present application can efficiently kill Enterococcus gallinarum and has specificity.

The invention belongs to the technical field of biology, and particularly discloses scophthalmus maximus-derived antibacterial protein SmH2A.1 as well as a preparation method and application of the scophthalmus maximus-derived antibacterial protein SmH2A.1. The amino acid sequence of the scophthalmus maximus antibacterial protein SmH2A.1 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the gene for coding the scophthalmus maximus antibacterial protein SmH2A.1 is as shown in SEQ ID NO. 2. The scophthalmus maximus antibacterial protein SmH2A.1 has a remarkable antibacterial effect on gram-negative bacterium escherichia coli and gram-positive bacterium staphylococcus aureus. In addition, in the presence of Ca < 2 + >, the scophthalmus maximus antibacterial protein SmH2A.1 can enable the two bacteria to generate agglutination reaction. The preparation method of the broad-spectrum antibacterial protein is simple, the broad-spectrum antibacterial protein can replace traditional antibiotics, and various common bacterial diseases in aquaculture can be quickly and efficiently prevented and treated.

Provided is a use of an antibacterial protein in a product for preventing and treating acne, or an infection caused by Propionibacterium acnes, or a condition related to Propionibacterium acnes, wherein the antibacterial protein comprises an amino acid sequence as shown in SEQ ID NO. 6, or an active fragment or analog thereof. The active fragment or analog can inhibit the growth of Propionibacterium acnes. The provided antibacterial protein can specifically and effectively kill Propionibacterium acnes without affecting hosts and other common skin microorganisms, can provide a safe and effective acne solution, and is suitable for long-term use without causing the risks of skin microecological imbalance and microbial drug resistance.

The invention relates to a recombinant expression bacteriostatic protein construct, an expression vector, a recombinant alcohol yeast, a preparation method and application, the construct provided by the invention sequentially comprises a promoter, a bacteriostatic protein gene and a terminator from the 5'end to the 3 'end, the nucleotide sequence of the bacteriostatic protein gene is SEQ ID NO.5, and the promoter is selected from one or two of ENO1p and ADH1p. When the recombinant alcohol yeast provided by the invention is used for alcoholic fermentation, the synchronously generated bacteriostatic protein can inhibit infectious microbes in the fermentation process to obtain high-yield ethanol.

The invention discloses a bacteriophage lyase Lys162 and an application of the bacteriophage lyase Lys162 in preparation of an antibacterial product. The bacteriophage lyase has a remarkable splitting effect on gram-negative bacteria and gram-positive bacteria, so that the bacteriophage lyase can be used as a broad-spectrum antibacterial protein. For example, the antibacterial effect on pseudomonas aeruginosa, klebsiella pneumoniae and streptococcus is remarkable. The antibacterial protein has relatively high temperature tolerance and pH tolerance, can replace antibiotics, and is applied to prevention and treatment of bacterial diseases as an external medicine or an internal medicine and sterilization of medical instruments and medical places; and the product can also be used as an additive to be applied to the fields of feed processing, food industry, animal husbandry, beer industry, aquaculture, cosmetic production, fruit preservation and the like.

The invention relates to a tomato-source antibacterial protein, application of the tomato-source antibacterial protein to prevention and treatment of tomato gray mold and a biological preparation for preventing and treating the tomato gray mold, and belongs to the technical field of tomato disease prevention and treatment. In order to solve the problem that a tomato-derived antibacterial protein for preventing and treating tomato gray mold is lacked in the prior art, the invention provides a tomato ABS258 antibacterial protein and a tomato ABS121 antibacterial protein, and the amino acid sequence of the tomato ABS121 antibacterial protein is from the first site to the 121th site of the amino acid sequence of the tomato ABS258 antibacterial protein. The specific botrytis cinerea resistant protein provided by the invention can effectively inhibit the growth of botrytis cinerea hyphae at low concentration and prevent pathogenic bacteria from infecting tomato leaves, and has the natural advantages of clear gene source and high compatibility with tomatoes, and the differentiated protein structure provides flexible choices for pesticide development aiming at different application scenes.

The invention discloses bacteriophage lyase and application thereof in preparation of an antibacterial agent. The bacteriophage lyase has a remarkable splitting effect on gram-negative bacteria, so that the bacteriophage lyase can be used as a broad-spectrum antibacterial protein. For example, the antibacterial effect on gram-negative bacteria such as escherichia coli, pseudomonas aeruginosa and klebsiella pneumoniae is remarkable. The antibacterial protein has relatively high temperature tolerance and pH tolerance, can replace antibiotics, and is applied to prevention and treatment of bacterial diseases as an external medicine or an internal medicine and sterilization of medical instruments and medical places; and the product can also be used as an additive to be applied to the fields of feed processing, food industry, animal husbandry, beer industry, aquaculture, cosmetic production, fruit preservation and the like.

A freeze-dried formulation includes an antibacterial protein having a killing activity specific to at least one or all of the following genera: Staphylococcus arlettae, Staphylococcus aureus, Staphylococcus auricularis, Staphylococcus carnosus, Staphylococcus caprae, Staphylococcus chromogenes, Staphylococcus cohnii, Staphylococcus delphini, Staphylococcus epidermidis, Staphylococcus equorum, Staphylococcus gallinarum, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus intermedius, Staphylococcus kloosii, Staphylococcus lentus, Staphylococcus lugdunensis, Staphylococcus muscae, Staphylococcus pasteuri, Staphylococcus saprophyticus, Staphylococcus warneri, and Staphylococcus xylosus; a poloxamer; a sugar; and an amino acid.