105 results about "Staphylococcus epidermidis" patented technology

The present invention relates to the use of a combination of a saccharide and glycerol for skin protection against undesirable bacteria. The present invention is particularly useful in the formulation of compositions that act as prebiotics for commensal skin bacteria such as S. epidermidis to produce metabolites such as lactic acid, which, through the present invention, has been shown to inhibit the growth of undesirable bacteria such as E. coli and S. aureus, among others. The present invention is especially useful because it provides both short-term (i.e., rapid) and long-term (or sustained) protection.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The present invention relates to the field of biological fermentation application technology, and in particular, to use of a milk peptide fermentation product in regulating skin microecological balance. The milk peptide fermentation product selectively regulates the growth of Staphylococcus epidermidis and inhibits the growth of Staphylococcus aureus and Escherichia coli. It was discovered for the first time that the milk peptide fermentation product has a selective bacteriostatic effect on skin microorganisms. The experimental results show that the milk peptide fermentation product has an inhibitory effect on Escherichia coli and Staphylococcus aureus, but has no effect on the normal growth of Staphylococcus epidermidis. This characteristic is obviously different from common broad-spectrum chemical bacteriostatic agents, and the use of the milk peptide fermentation product is more conducive to maintaining the balance of skin microbiota.

The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (Δalr1 Δalr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium. In another aspect, the disclosure features a method of treating or preventing a rash in a subject, comprising administering to the subject a population of the recombinant Staphylococcus bacterium of any one of the aspects or embodiments described herein, in an effective amount to treat or prevent the rash in the subject.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention belongs to the technical field of probiotics, and particularly relates to plant lactobacillus with bacteriostatic activity and application of the plant lactobacillus. Wherein the plant lactobacillus with bacteriostatic activity is separated from the intestinal tract of a healthy human body, and the LV1000 fermentation filtrate freeze-dried powder is prepared through fermentation, centrifugation, filtration, ultrafiltration concentration and vacuum freeze-drying by taking inactivated staphylococcus epidermidis bacterial suspension as an inducer through a specific induction fermentation culture medium. Meanwhile, the freeze-dried powder is compounded with an embedding compound, galactooligosaccharide, beta-cyclodextrine, erythritol and the like, so that the fermented beverage capable of improving the intestinal microecology is prepared. The freeze-dried powder of the LV1000 fermentation filtrate can specifically inhibit enteropathogenic bacteria, the tea polyphenol in the embedded compound can further synergistically enhance the bacteriostatic action and exert the antioxidant effect, and the fermented beverage which is high in stability, fine and smooth in taste, free of refrigeration and capable of being directly drunk and improving the intestinal microecology is prepared.

To provide a composition effective for treating microbial skin infections, atopic dermatitis and the like.SOLUTION: Methods and compositions comprising hogocidin peptides (SH-lantibiotics), derivatives and variants are provided. Also provided are methods and compositions, including probiotic compositions, that utilize strains of Staphylococcus hominis and Staphylococcus epidermidis that produce hogocidin, hogocidin-like peptides, or other inhibitors of skin pathogens. Methods of treating microbial skin infections and atopic dermatitis are also provided.SELECTED DRAWING: Figure 1A-B

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 9I and 16F replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-11. Compared to the template peptide PL-0, the stapling peptide PL-11 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

Described is a method for treating or preventing an infection, such as an infection associated with Staphylococcus epidermidis such as a Health-Care Associated infection, a wound, a sore, an ulcer, a burn. The method includes applying a treatment preparation to a treatment site, the treatment preparation has a first component having a single amino acid, said single amino acid being leucine, and a second component having an active chlorine compound.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 6V and 13V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-10. Compared to the template peptide PL-0, the stapling peptide PL-10 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of a bacillus subtilis antibacterial peptide. By accurately controlling the inoculation density culture in the logarithmic phase, the biomass density of the thalli can be increased by 2-3 times, and the fermentation period is shortened; by optimizing the composition of a fermentation culture medium, fermentation conditions and a downstream treatment method, the yield and activity of the bacillus subtilis antibacterial peptide can be remarkably improved, the broad-spectrum bacteriostasis is enhanced, the drug resistance is remarkable, and the bacillus subtilis antibacterial peptide has the tendency of selective intelligent bacteriostasis due to low concentration, namely, the bacillus subtilis antibacterial peptide has no obvious inhibition effect on skin beneficial bacteria staphylococcus epidermidis; and the traditional Chinese medicine composition has a relatively obvious inhibition effect on staphylococcus aureus, propionibacterium acnes and other strains. Moreover, only water, inorganic salt and food-grade raw materials are used in the whole process, no organic solvent is left, and compared with a traditional ethanol / acetone extraction method, the method better meets the FDA / EFSA standard.

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of cosmetics. The invention provides a plant composition which is prepared from magnolia bark and uncaria rhynchophylla, and the feeding ratio of the magnolia bark to the uncaria rhynchophylla is 7: 3-3: 7. The plant composition has the effect of synergistically promoting expression of scalp keratinocyte human beta-defensin, has an inhibiting effect on malassezia and does not have an inhibiting effect on staphylococcus epidermidis under the concentration of 0.5%-2%, has the effect of adjusting scalp flora balance, and can relieve the degree of scalp itching and reduce the number of scurf.

The invention belongs to the technical field of biology, and particularly relates to an in-situ spray polymerization coating started by bacterial exovesicles (LBEVs) as well as a preparation method and application of the in-situ spray polymerization coating. And functionalizing the LBEVs by Fe < 3 + > through electrostatic adsorption to obtain an LBEVs-Fe < 3 + > solution. After LBEVs-Fe < 3 + > and pyrrole monomers are sprayed on a wound part, the LBEVs-Fe < 3 + > initiates pyrrole to be oxidized and polymerized into polypyrrole, and a polymerized coating LBEVs-PPy is formed. The LBEVs show effective antibacterial activity on staphylococcus aureus, escherichia coli and staphylococcus epidermidis, near-infrared triggered photo-thermal activation generates mild thermal therapy, expression of angiogenesis regulatory factors (such as VEGFA and ANGPT1) in human umbilical vein endothelial cells (HUVECs) is remarkably up-regulated, and an HSP90 / p-eNOS pathway is activated to accelerate angiogenesis, so that the activity of the LBEVs is improved. The LBEVs-PPy coating remarkably accelerates wound healing through a synergistic mechanism integrating the strong antibacterial activity of LBEVs and the efficient photo-thermal characteristic of PPy.

The invention discloses torreya grandis seed oil and application thereof, and belongs to the field of plant extracts. The torreya grandis seed oil is prepared by adopting a supercritical CO2 extraction method, the expression level of a macrophage RAW264.7 inflammatory factor IL-6 caused by LPS can be remarkably reduced, and the torreya grandis seed oil has a good anti-inflammatory effect. The traditional Chinese medicine composition disclosed by the invention can effectively inhibit the growth of propionibacterium acnes, also can effectively regulate the skin flora balance of people with acnes, has no obvious influence on the growth of staphylococcus epidermidis while remarkably inhibiting the growth of propionibacterium acnes, is an excellent acne-removing cosmetic raw material, and has a wide application prospect in the development of nursing products for people with acnes.

The invention discloses staphylococcus epidermidis CCSM009 for repairing a skin barrier and a metagen of the staphylococcus epidermidis CCSM009, and belongs to the field of microbial technologies and medicines. The staphylococcus epidermidis CCSM009 and the metagen thereof provided by the invention have a good barrier function repairing effect and application prospect in the aspect of external use, and are specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, and the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved; meanwhile, sphingomyelinase 1 and tissue kininolase 7 (KLK7) are improved, and it is clear that the bacterium performs up-regulation of skin barrier related proteins and enzymes through an AHR-OVOL1 pathway. Therefore, the metagen of the staphylococcus epidermidis CCSM009 has a huge application prospect in preparation of cosmetics or medicines for repairing the skin barrier.

The invention discloses a fusion membrane of staphylococcus epidermidis vesicles and turmeric derived exosome-like vesicles as well as a preparation method and application of the fusion membrane. Staphylococcus epidermidis vesicles and turmeric-derived exosome-like vesicles are fused to obtain HMVs, the antibacterial effect of the HMVs is verified through in-vitro experiments, then the HMVs are loaded in hyaluronic acid microneedles, the HMVs MNs nano system can be pressed on acne skin lesions for local administration, and Balb / c mouse in-vivo experiments prove that the HMVs MNs nano system has remarkable antibacterial and anti-inflammatory curative effects. Therefore, the HMVs MNs have good application potential in treating acne.

The invention discloses a staphylococcus epidermidis fermentation product, a preparation method thereof and application of the staphylococcus epidermidis fermentation product in preparation of anti-inflammatory drugs. The method comprises the following steps: inoculating staphylococcus epidermidis into a TSB culture medium for culture, and after culture is finished, sequentially centrifuging and filtering the obtained fermentation liquor to finally obtain fermentation supernate, namely a staphylococcus epidermidis fermentation product. It is found that the optimized staphylococcus epidermidis fermentation product has an anti-inflammatory effect, generation of proinflammatory factors such as NO, IL-6 and TNF-alpha can be remarkably reduced, and therefore the staphylococcus epidermidis fermentation product can be applied to the industry of research and development of soothing cosmetics, and the effect of the product is enhanced.

The invention relates to the field of daily chemicals, and discloses a plant-derived sterilization deodorant and application thereof in daily chemical products. The sterilizing deodorant comprises at least two, preferably three, of pepper leaf extract, celery leaf extract and syzygium jambos leaf extract, and is matched with decyl glucoside, citric acid, a chelating agent and an optional deep eutectic solvent to form a stable water-alcohol system. Through specific proportion compounding and process condition control, efficient bacteriostasis on staphylococcus aureus, staphylococcus epidermidis, candida albicans and trichophyton rubrum is achieved, meanwhile, the concentration of odor substances is remarkably reduced, and the deodorant has the functions of source bacteriostasis and direct deodorization. The physical stability and the antibacterial activity retention rate of the system are further improved by adopting a deep eutectic solvent composed of glycerol and choline chloride, so that the deep eutectic solvent is suitable for preparing daily chemical products such as shoe and sock spray, body spray and fabric freshener.

The present invention relates to a method for preparing an extract of at least one bacterium of the species Staphylococcus epidermidis comprising at least one step of culturing at least one bacterium of the species Staphylococcus epidermidis in the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement. The present invention is also directed to an extract of at least one bacterium of the species Staphylococcus epidermidis, preferably an extract of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under the serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, obtainable, obtained or directly obtained by the abovementioned preparation method. The present invention is further directed to a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under the serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695. The present invention also relates to cosmetic treatment methods and cosmetic uses of said extracts for caring for keratin materials, for preventing and / or treating signs of aging of keratin materials, and as active anti-aging agent.

The application discloses a natural product 3-methylcarbazole derivative with a general formula B and a preparation method and application thereof in the technical field of organic chemistry. S. aureus S. epidermidis The derivative has good antibacterial activity, can significantly inhibit Staphylococcus aureus, Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus (MRSA), and can be used for preparing a drug for treating related bacterial infections.​

The invention relates to the technical field of cosmetics, and provides skin repair essence taking a staphylococcus epidermidis fermentation product as a core, the skin repair essence is prepared from a core repair component, a moisturizing and soothing component and a mild auxiliary component in a synergistic compatibility manner by weight: the core repair component comprises 1.0-10.0% of staphylococcus epidermidis fermentation product filtrate and 0.5-2.0% of Ectoine; a moisturizing and soothing component: 0.1 to 0.5 percent of small molecular sodium hyaluronate, 0.2 to 1.0 percent of panthenol, 0.3 to 1.5 percent of beta-glucan and 5.0 to 15.0 percent of a moisturizing agent; and a mild auxiliary component: 0.3-0.8% of a mild preservative, and the balance of deionized water. The staphylococcus epidermidis fermentation product is taken as a core to be compounded with multiple repairing components to form a synergistic system, so that the skin micro-ecology can be regulated, harmful bacteria can be inhibited, damaged barriers can be repaired, deep moisturizing can be realized, and red discomforts can be relieved. The formula is mild and non-irritant, is adaptive to sensitive skin and scenes after medical art, is stable and controllable in process, fully covers multiple scenes, improves skin stability from the source, and is superior to traditional products in use experience and repair effect.

This invention belongs to the field of peptide drugs, specifically relating to an antibacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 13V and 20V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-13. Compared to the template peptide PL-0, the stapling peptide PL-13 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The invention belongs to the field of polypeptide drugs, and particularly relates to an antibacterial polypeptide and application thereof. According to the invention, Rink amide MBHA amino resin is used as a solid phase carrier, modification and transformation are carried out according to an amino acid sequence of a template polypeptide raniseptinPL: Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2, and on the basis of retaining key amino acid residues, original amino acids are respectively replaced by S5 and R8 at amino acid positions i, i + 4 and i and i + 7, so that a target stapling peptide is obtained. Compared with a template polypeptide PL-0, the obtained stapled peptide has the advantage that the antibacterial activity on staphylococcus aureus, enterococcus faecium, enterococcus faecalis and staphylococcus epidermidis can be obviously improved.

A thermally responsive nanosphere for binding to biofilms including a gold nanoparticle core functionalized with a polyethylene glycol; an R2ab fusion protein construct including an anchor that is a stable globular domain having a surface accessible cysteine residue, a linker, an S. epidermidis R2ab protein of SEQ ID NO. 1, and an elastin-like polypeptide. The linker may be a protein having SEQ ID NO. 7; and the anchor may be a modified third IgG binding domain from streptococcal protein G (GB3) having SEQ ID NO. 3, a ubiquitin protein having SEQ ID NO. 4, a Pin1 WW domain having SEQ ID NO. 5, and a fibronectin protein domain 3FN3 having SEQ ID NO. 6. A method for treating a biofilm containing S. epidermidis bacteria by binding a plurality of the nanospheres to the biofilm and exposing the biofilm to laser irradiation.

The application belongs to the field of polypeptide drugs, and particularly relates to an antibacterial polypeptide and application thereof in pharmacy. The application takes Rink amide MBHA amino resin as a solid phase carrier, and modifies and transforms according to a template polypeptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 amino acid sequence, wherein amino acid residues 19G and 26N are replaced by R8 and S5, to obtain a target stapled peptide PL-16. The stapled peptide PL-16 can significantly improve the antibacterial activity on Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis and Staphylococcus epidermidis relative to the template polypeptide PL-0.

The invention relates to a hair care and / or scalp care composition comprising rhamnose, trehalose and lactobionic acid. Said composition moisturizes the scalp, protects the scalp from dehydration, promotes the growth of Staphylococcus epidermis and / or Cutibacterium acnes on the scalp and can be used for treating or preventing dandruff.

Blepharitis, an infection and inflammation of the edge of the eyelids, and meibomian gland dysfunction (MGD), blockage of tiny oil glands at the eye lid margin, are quite common, usually under diagnosed and underappreciated diseases. There are number of causes for blepharitis, including bacterial components such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Corynebacteria sp., seborrhea or microscopic mites (Demodex). More than 50% of patients seeking ophthalmologic consultations have symptoms and signs indicating one of these entities. Another large number of patients remain undiagnosed or in early stages of disease that could be easily treated thus preventing long-term pain and suffering. MGD is in fact the leading cause of dry eye disease, a potentially preventable malady.

The application discloses a fusion membrane of Staphylococcus epidermidis vesicles and curcumin-derived exosome-like vesicles and a preparation method and application thereof. The application fuses Staphylococcus epidermidis vesicles and curcumin-derived exosome-like vesicles to obtain HMVs, and verifies the antibacterial effect of the HMVs through in-vitro experiments, and then the HMVs are loaded in hyaluronic acid microneedles. The HMVs MNs nano system can be locally administered by pressing on acne lesions, and the in-vivo experiments on Balb / c mice prove that the system has relatively remarkable antibacterial and anti-inflammatory effects. Therefore, the HMVs MNs have relatively good application potential in treating acne.

The invention relates to an antibacterial peptide TroLEAP2-21 and application of the antibacterial peptide TroLEAP2-21 in preparation of products for resisting bacteria and maintaining fish tissue integrity, and belongs to the technical field of biological polypeptides, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The invention also provides an application of the trachinotus ovatus antibacterial peptide TroLEAP2-21 in preparation of antibacterial products, and the bacteria are lactococcus gasseri and staphylococcus epidermidis. The antibacterial peptide TroLEAP2-21 can change the permeability of a bacterial cell membrane, induce depolarization of the bacterial cell membrane, degrade bacterial genome DNA (Deoxyribonucleic Acid) and cause bacterial morphological change, so that an antibacterial effect is achieved.