133 results about "Staphylococcus epidermidis" patented technology

The invention relates to Penicillium decumbens C01 and application of the Penicillium decumbens C01, and belongs to the technical field of microorganisms. The penicillium decumbens C01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the penicillium decumbens C01 is GDMCC NO.66456. The strain is obtained by separating, purifying and screening soil samples collected from botanical gardens in South China of Guangzhou; tests show that the bacteriostatic active substance generated by fermentation of the strain has a good bacteriostatic effect on staphylococcus epidermidis, staphylococcus aureus, escherichia coli, propionibacterium acnes, candida albicans and pseudomonas aeruginosa, and the bacteriostatic active substance has the characteristic of high temperature resistance and excellent preservative effect; the antibacterial preservative can be used as an antibacterial preservative raw material, is applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention belongs to the technical field of polypeptide drugs in biochemistry, and discloses three antibacterial peptide analogues as well as a preparation method and application thereof, the sequence of an antibacterial peptide P-1 is CH3CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-2 is CH3CH2CH2CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-3 is CH3 (CH2) 6CO-GGGENIKKILSKIKLLK-NH2, the carboxyl ends of polypeptides are subjected to amidation, and the polypeptides are all positively charged when the pH is equal to 7. The artificially synthesized antibacterial peptide disclosed by the invention shows a remarkable sterilization effect on multidrug resistant staphylococcus epidermidis. P-1, P-2 and P-3 have small molecular weight, are convenient to synthesize, have broad-spectrum bactericidal activity, are self-assembled into nanoparticles, have low cytotoxicity, resist trypsin degradation and the like, and have wide application prospects.

The invention discloses glucoside hydrolase with anti-biofilm activity and application of the glucoside hydrolase. Experiments prove that the PgaBAb1 protein and the PgaBKp protein have remarkable anti-staphylococcus epidermidis biofilm activity and can destroy a mature staphylococcus epidermidis biofilm and inhibit formation of the staphylococcus epidermidis biofilm, that is, the PgaBAb1 protein and the PgaBKp protein are glucoside hydrolase with anti-biofilm activity; meanwhile, the sensitivity of the staphylococcus epidermidis to methicillin can be improved; the amino acid sequences of the PgaBAb1 protein and the PgaBKp protein are respectively as shown in SEQ ID NO: 5 and SEQ ID NO: 6. Therefore, both the PgaBAb1 protein and the PgaBKp protein can be used as active ingredients of drugs to treat diseases caused by infection of bacteria generating biological membranes and / or drug-resistant bacteria. The method has an important application value.

The present invention relates to the use of a combination of a saccharide and glycerol for skin protection against undesirable bacteria. The present invention is particularly useful in the formulation of compositions that act as prebiotics for commensal skin bacteria such as S. epidermidis to produce metabolites such as lactic acid, which, through the present invention, has been shown to inhibit the growth of undesirable bacteria such as E. coli and S. aureus, among others. The present invention is especially useful because it provides both short-term (i.e., rapid) and long-term (or sustained) protection.

The present application relates to a strain of Penicillium decumbens (P. Penicillium decumbens ) C01 and its application, and belongs to the field of microbial technology. The Penicillium decumbens C01 disclosed by the present application has been preserved in the Guangdong Microbial Digital Culture Collection Center, and its preservation number is GDMCC NO.66456. The strain is isolated, purified and screened from a soil sample collected in the garden area of Guangzhou South China Botanical Garden. Tests show that the bacteriostatic active substance produced by fermentation of the strain has good bacteriostatic effect on Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, Candida albicans and Pseudomonas aeruginosa. Moreover, the bacteriostatic active substance has high-temperature resistance and excellent preservative effect, and can be used as a bacteriostatic and preservative raw material, applied in the fields of food, health care products or daily chemical products, and has broad application prospect and important transformation research value.

The invention discloses a mild antibacterial anti-dandruff composition and a preparation method thereof. The composition comprises an acrylate copolymer, maltooligosaccharide and bisabolol, the mass ratio of the acrylate copolymer to the maltooligosaccharide to the bisabolol is (0.01 to 0.3) to (0.1 to 0.8) to (0.05 to 0.5); the preparation method comprises the following steps: sequentially adding the acrylate copolymer, the maltooligosaccharide and the bisabolol into water according to the mass ratio, and uniformly stirring until the materials are dissolved. The acrylate copolymer, the maltooligosaccharide and the bisabolol are blended in proportion, the maltooligosaccharide becomes a carbon source of microorganisms, malassezia is efficiently inhibited, staphylococcus epidermidis is not excessively inhibited, and meanwhile, the skin mildness and moisture retention are achieved; the bisabolol is used as a plasticizer, so that the composition of the acrylate copolymer and the maltooligosaccharide forms a uniform and tough film, the effect on malassezia and staphylococcus epidermidis is enhanced, and the anti-dandruff effect is improved.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention relates to a biomarker for individual recognition and application thereof, and relates to the technical field of biology. The biomarker comprises at least one of the following microorganisms: propionibacterium acnes, staphylococcus epidermidis, marine paracoccus, streptococcus, rhodococcus fence celebrating, staphylococcus, pseudomonas, streptococcus sanguineus, achromobacter xylosoxidans, acinetobacter calcoaceticus, acinetobacter johnsonii, staphylococcus lentus, pseudomonas toralaris and brevundimonas vesicularis. Or enterococcus faecium. The biomarker can provide analysis with higher resolution in the aspect of differential strain information, has a better distinguishing effect, higher accuracy and sensitivity on individual recognition, and can be used as a new technical means for supplementing forensic individual recognition.

The present invention relates to the field of biological fermentation application technology, and in particular, to use of a milk peptide fermentation product in regulating skin microecological balance. The milk peptide fermentation product selectively regulates the growth of Staphylococcus epidermidis and inhibits the growth of Staphylococcus aureus and Escherichia coli. It was discovered for the first time that the milk peptide fermentation product has a selective bacteriostatic effect on skin microorganisms. The experimental results show that the milk peptide fermentation product has an inhibitory effect on Escherichia coli and Staphylococcus aureus, but has no effect on the normal growth of Staphylococcus epidermidis. This characteristic is obviously different from common broad-spectrum chemical bacteriostatic agents, and the use of the milk peptide fermentation product is more conducive to maintaining the balance of skin microbiota.

The invention provides an application of GSK2018682 in preparation of a medicine for resisting gram-positive bacterial infection, and the GSK2018682 has a CAS number of 1034688-30-6. The invention also provides an application of the GSK2018682 in preparation of a medicine for resisting gram-positive bacterial infection. According to the technical scheme, the novel medical application of the GSK2018682 is disclosed, and the GSK2018682 has good antibacterial activity and anti-biofilm activity on various gram-positive bacteria and can inhibit growth of staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium and the like and formation of biofilms.

The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (Δalr1 Δalr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium. In another aspect, the disclosure features a method of treating or preventing a rash in a subject, comprising administering to the subject a population of the recombinant Staphylococcus bacterium of any one of the aspects or embodiments described herein, in an effective amount to treat or prevent the rash in the subject.

The invention provides a beta-diketoamide compound and application thereof in preparation of a gram infection resistant drug, the structural formula of the beta-diketoamide compound is as shown in formula (1), in the formula (1), R1 is H or halogen; r2 is nitro, alkyl, halogen or methoxyl. The beta-diketoamide compound disclosed by the invention comprises gram-positive bacteria such as staphylococcus aureus, enterococcus faecalis, enterococcus faecium and staphylococcus epidermidis and other clinical isolates, and shows antibacterial activity; after being combined with polymyxin, the compound has synergistic bacteriostatic activity on gram-negative bacteria such as acinetobacter baumannii, klebsiella pneumoniae, escherichia coli and pseudomonas aeruginosa, and can remove biofilms formed by staphylococcus aureus and enterococcus faecalis.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

A bithionol derivative, and a preparation method therefor and a use thereof. The bithionol derivative is as represented by any one of chemical structural formulas I-1 to I-7. The bithionol derivative as represented by the structural formula exhibits better bacteriostatic activity against clinical isolates of gram-positive bacteria such as Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Staphylococcus epidermidis and the effect of inhibiting the formation of Staphylococcus aureus biofilms. As compared with bithionol and other bithionol derivatives, the bithionol derivative as represented by the structural formula has a lower inhibitory concentration, a better antibacterial effect, and potential application value in clinical antibacterial infection treatment.

The invention belongs to the technical field of probiotics, and particularly relates to plant lactobacillus with bacteriostatic activity and application of the plant lactobacillus. Wherein the plant lactobacillus with bacteriostatic activity is separated from the intestinal tract of a healthy human body, and the LV1000 fermentation filtrate freeze-dried powder is prepared through fermentation, centrifugation, filtration, ultrafiltration concentration and vacuum freeze-drying by taking inactivated staphylococcus epidermidis bacterial suspension as an inducer through a specific induction fermentation culture medium. Meanwhile, the freeze-dried powder is compounded with an embedding compound, galactooligosaccharide, beta-cyclodextrine, erythritol and the like, so that the fermented beverage capable of improving the intestinal microecology is prepared. The freeze-dried powder of the LV1000 fermentation filtrate can specifically inhibit enteropathogenic bacteria, the tea polyphenol in the embedded compound can further synergistically enhance the bacteriostatic action and exert the antioxidant effect, and the fermented beverage which is high in stability, fine and smooth in taste, free of refrigeration and capable of being directly drunk and improving the intestinal microecology is prepared.

To provide a composition effective for treating microbial skin infections, atopic dermatitis and the like.SOLUTION: Methods and compositions comprising hogocidin peptides (SH-lantibiotics), derivatives and variants are provided. Also provided are methods and compositions, including probiotic compositions, that utilize strains of Staphylococcus hominis and Staphylococcus epidermidis that produce hogocidin, hogocidin-like peptides, or other inhibitors of skin pathogens. Methods of treating microbial skin infections and atopic dermatitis are also provided.SELECTED DRAWING: Figure 1A-B

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 9I and 16F replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-11. Compared to the template peptide PL-0, the stapling peptide PL-11 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

Described is a method for treating or preventing an infection, such as an infection associated with Staphylococcus epidermidis such as a Health-Care Associated infection, a wound, a sore, an ulcer, a burn. The method includes applying a treatment preparation to a treatment site, the treatment preparation has a first component having a single amino acid, said single amino acid being leucine, and a second component having an active chlorine compound.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 6V and 13V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-10. Compared to the template peptide PL-0, the stapling peptide PL-10 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

The invention belongs to the technical field of microorganisms, and finds that trans-cinnamic acid is a key metabolite of microorganism staphylococcus epidermidis H62-3 for the first time, so that a new method is provided for preparation of the trans-cinnamic acid, and meanwhile, the problem of environmental pollution caused by chemical synthesis of the trans-cinnamic acid is also avoided. In addition, the invention also relates to an application of trans-cinnamic acid in preparation of a product for resisting skin photoaging. The trans-cinnamic acid provided by the invention has the effect of resisting skin photoaging, can effectively relieve the transcriptional level of senescence-related genes p53, p21 and p16 and inflammatory factors IL-1beta, IL-6 and TNF-alpha induced by UVB irradiation, reduces the level of reactive oxygen species (ROS), inhibits the expression of matrix metalloproteinases MMP9, MMP3 and MMP1, promotes the synthesis of I-type collagen (COL1), and can be used for preparing anti-aging drugs for treating skin aging. And the activity of beta-galactosidase (SA-beta-gal) related to aging is reduced.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of a bacillus subtilis antibacterial peptide. By accurately controlling the inoculation density culture in the logarithmic phase, the biomass density of the thalli can be increased by 2-3 times, and the fermentation period is shortened; by optimizing the composition of a fermentation culture medium, fermentation conditions and a downstream treatment method, the yield and activity of the bacillus subtilis antibacterial peptide can be remarkably improved, the broad-spectrum bacteriostasis is enhanced, the drug resistance is remarkable, and the bacillus subtilis antibacterial peptide has the tendency of selective intelligent bacteriostasis due to low concentration, namely, the bacillus subtilis antibacterial peptide has no obvious inhibition effect on skin beneficial bacteria staphylococcus epidermidis; and the traditional Chinese medicine composition has a relatively obvious inhibition effect on staphylococcus aureus, propionibacterium acnes and other strains. Moreover, only water, inorganic salt and food-grade raw materials are used in the whole process, no organic solvent is left, and compared with a traditional ethanol / acetone extraction method, the method better meets the FDA / EFSA standard.

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of cosmetics. The invention provides a plant composition which is prepared from magnolia bark and uncaria rhynchophylla, and the feeding ratio of the magnolia bark to the uncaria rhynchophylla is 7: 3-3: 7. The plant composition has the effect of synergistically promoting expression of scalp keratinocyte human beta-defensin, has an inhibiting effect on malassezia and does not have an inhibiting effect on staphylococcus epidermidis under the concentration of 0.5%-2%, has the effect of adjusting scalp flora balance, and can relieve the degree of scalp itching and reduce the number of scurf.

The invention belongs to the technical field of biology, and particularly relates to an in-situ spray polymerization coating started by bacterial exovesicles (LBEVs) as well as a preparation method and application of the in-situ spray polymerization coating. And functionalizing the LBEVs by Fe < 3 + > through electrostatic adsorption to obtain an LBEVs-Fe < 3 + > solution. After LBEVs-Fe < 3 + > and pyrrole monomers are sprayed on a wound part, the LBEVs-Fe < 3 + > initiates pyrrole to be oxidized and polymerized into polypyrrole, and a polymerized coating LBEVs-PPy is formed. The LBEVs show effective antibacterial activity on staphylococcus aureus, escherichia coli and staphylococcus epidermidis, near-infrared triggered photo-thermal activation generates mild thermal therapy, expression of angiogenesis regulatory factors (such as VEGFA and ANGPT1) in human umbilical vein endothelial cells (HUVECs) is remarkably up-regulated, and an HSP90 / p-eNOS pathway is activated to accelerate angiogenesis, so that the activity of the LBEVs is improved. The LBEVs-PPy coating remarkably accelerates wound healing through a synergistic mechanism integrating the strong antibacterial activity of LBEVs and the efficient photo-thermal characteristic of PPy.

The invention discloses torreya grandis seed oil and application thereof, and belongs to the field of plant extracts. The torreya grandis seed oil is prepared by adopting a supercritical CO2 extraction method, the expression level of a macrophage RAW264.7 inflammatory factor IL-6 caused by LPS can be remarkably reduced, and the torreya grandis seed oil has a good anti-inflammatory effect. The traditional Chinese medicine composition disclosed by the invention can effectively inhibit the growth of propionibacterium acnes, also can effectively regulate the skin flora balance of people with acnes, has no obvious influence on the growth of staphylococcus epidermidis while remarkably inhibiting the growth of propionibacterium acnes, is an excellent acne-removing cosmetic raw material, and has a wide application prospect in the development of nursing products for people with acnes.

The invention discloses staphylococcus epidermidis CCSM009 for repairing a skin barrier and a metagen of the staphylococcus epidermidis CCSM009, and belongs to the field of microbial technologies and medicines. The staphylococcus epidermidis CCSM009 and the metagen thereof provided by the invention have a good barrier function repairing effect and application prospect in the aspect of external use, and are specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, and the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved; meanwhile, sphingomyelinase 1 and tissue kininolase 7 (KLK7) are improved, and it is clear that the bacterium performs up-regulation of skin barrier related proteins and enzymes through an AHR-OVOL1 pathway. Therefore, the metagen of the staphylococcus epidermidis CCSM009 has a huge application prospect in preparation of cosmetics or medicines for repairing the skin barrier.

To provide a formulation including components contributing to epidermal and dermal health.SOLUTION: The present invention provides a bacterium belonging to Staphylococcus epidermidis species, strain BG-PG180, deposited as accession number NITE P-04076, which enhances expression of the ceramide synthase 3 gene and also enhances expression of the hyaluronan synthase 3 gene. A formulation prepared using this strain can enhance expression of the ceramide synthase 3 gene and also enhance expression of the hyaluronan synthase 3 gene.SELECTED DRAWING: None

The invention discloses a fusion membrane of staphylococcus epidermidis vesicles and turmeric derived exosome-like vesicles as well as a preparation method and application of the fusion membrane. Staphylococcus epidermidis vesicles and turmeric-derived exosome-like vesicles are fused to obtain HMVs, the antibacterial effect of the HMVs is verified through in-vitro experiments, then the HMVs are loaded in hyaluronic acid microneedles, the HMVs MNs nano system can be pressed on acne skin lesions for local administration, and Balb / c mouse in-vivo experiments prove that the HMVs MNs nano system has remarkable antibacterial and anti-inflammatory curative effects. Therefore, the HMVs MNs have good application potential in treating acne.

The invention discloses a staphylococcus epidermidis fermentation product, a preparation method thereof and application of the staphylococcus epidermidis fermentation product in preparation of anti-inflammatory drugs. The method comprises the following steps: inoculating staphylococcus epidermidis into a TSB culture medium for culture, and after culture is finished, sequentially centrifuging and filtering the obtained fermentation liquor to finally obtain fermentation supernate, namely a staphylococcus epidermidis fermentation product. It is found that the optimized staphylococcus epidermidis fermentation product has an anti-inflammatory effect, generation of proinflammatory factors such as NO, IL-6 and TNF-alpha can be remarkably reduced, and therefore the staphylococcus epidermidis fermentation product can be applied to the industry of research and development of soothing cosmetics, and the effect of the product is enhanced.

The invention relates to the field of daily chemicals, and discloses a plant-derived sterilization deodorant and application thereof in daily chemical products. The sterilizing deodorant comprises at least two, preferably three, of pepper leaf extract, celery leaf extract and syzygium jambos leaf extract, and is matched with decyl glucoside, citric acid, a chelating agent and an optional deep eutectic solvent to form a stable water-alcohol system. Through specific proportion compounding and process condition control, efficient bacteriostasis on staphylococcus aureus, staphylococcus epidermidis, candida albicans and trichophyton rubrum is achieved, meanwhile, the concentration of odor substances is remarkably reduced, and the deodorant has the functions of source bacteriostasis and direct deodorization. The physical stability and the antibacterial activity retention rate of the system are further improved by adopting a deep eutectic solvent composed of glycerol and choline chloride, so that the deep eutectic solvent is suitable for preparing daily chemical products such as shoe and sock spray, body spray and fabric freshener.