485results about How to "High antibacterial activity" patented technology

The invention discloses a broad-spectrum antibacterial peptide SS-7 and application thereof, and belongs to the field of biological medicine. The amino acid sequence of the broad-spectrum antibacterial peptide SS-7 disclosed by the invention is VRRVRFVVLRRIKKWRRI, and the broad-spectrum antibacterial peptide SS-7 has a remarkable inhibition effect on various gram-positive bacteria and gram-negative bacteria such as drug-resistant strains such as methicillin-resistant staphylococcus aureus (ATCC43300) and shows low toxicity and no hemolytic toxicity on eukaryotic cells and red blood cells of mammals; lPS and lipoteichoic acid on the surfaces of bacteria can be combined to achieve the effect of neutralizing the bacteria, and a very good treatment and protection effect is achieved on animals infected by the bacteria. The antibacterial peptide SS-7 can be used for preparing an antibacterial agent and preparing a medicine for preventing or treating bacterial infection, has the advantages of being wide in antibacterial spectrum, low in drug-resistant mutation frequency, low in toxicity and low in production cost, can effectively solve the current bacterial drug resistance problem, and has wide application prospects and important clinical value.

The invention discloses a composition for preventing hair loss, controlling oil and removing dandruff and a preparation method thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition for preventing hair loss, controlling oil and removing dandruff comprises the following components: 2-5 parts of a folium artemisiae argyi extract, 1.1-1.6 parts of a ginger root extract, 5-10 parts of a polygonum multiflorum extract and 4-6 parts of a cacumen biotae extract. The polygonum multiflorum extract prepared by the method disclosed by the invention has better bacteriostatic activity, and compared with single strain fermentation, the mixed strain fermentation can more effectively enrich active ingredients in polygonum multiflorum; the anti-hair-loss, oil-controlling and anti-dandruff composition provided by the invention has excellent oil-controlling and oil-inhibiting effects; the shampoo provided by the invention has remarkable effects of preventing hair loss and strengthening hair.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant acinetobacter baumannii as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide is cd77AHP-2, and the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1. The antibacterial peptide cd77 obtained through artificial chemical synthesis is modified, distribution of cationic amino acid is optimized, and the antibacterial activity of the antibacterial peptide is remarkably improved. The antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on acinetobacter baumannii which are gram-negative bacteria and multi-drug resistant acinetobacter baumannii, and the antibacterial peptide cd77AHP-2 has a remarkable antibacterial effect on the acinetobacter baumannii which are gram-negative bacteria. The antibacterial peptide cd77AHP-2 provided by the invention has relatively low cytotoxicity to human bronchial epithelial cells, and has the potential of preparing a drug for resisting drug-resistant acinetobacter baumannii. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.

The invention discloses an antibacterial peptide SAMP4 or a derivative thereof, a pharmaceutical composition and application, and belongs to the technical field of biology, the amino acid sequence of the antibacterial peptide SAMP4 is as shown in SEQ ID NO: 1, and the antibacterial peptide SAMP4 has a broad-spectrum antibacterial effect and can be used for preparing antibacterial peptide SAMP4. The compound has a strong bacteriostatic effect on various gram-positive bacteria such as staphylococcus aureus, enterococcus and streptococcus and various gram-negative bacteria such as escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa, and is strong in antibacterial activity, wide in antibacterial spectrum and low in toxicity. The invention also provides a derivative and a salt of the antibacterial peptide SAMP4 and application of the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 to research, development and preparation of various antibacterial products or / and antibacterial drugs and treatment of bacterial infectious diseases, and the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 are remarkable in advantages and wide in application prospect.

The invention discloses lactococcus gasseri C6a2 and application thereof. The preservation number of the lactococcus gasseri C6a2 is CGMCC (China General Microbiological Culture Collection Center) NO: 31024; the 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the lactococcus gasseri C6a2 is as shown in SEQ ID NO: 1. The bacterial strain is a macrobrachium rosenbergii intestinal absolute dominant bacterium, has a typical probiotic function, is high in growth speed and activity, can quickly enter a logarithmic phase, has relatively high acid production and acid resistance, can secrete lipase, has relatively high bacteriostatic activity on staphylococcus aureus, aeromonas hydrophila and aeromonas veronii, and can be used for preparing the macrobrachium rosenbergii. The bacterium can be used for preparing a special probiotic preparation for macrobrachium rosenbergii, so that the sustainable development of the macrobrachium rosenbergii industry in China is promoted.

The invention discloses carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops as well as a preparation method and application thereof, and relates to the technical field of eye drops and medical biogel. The carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops are prepared from tannic acid modified carboxymethyl chitosan and adipose tissue-derived stromal cell exosome freeze-dried powder, the carboxymethyl chitosan-hyaluronic acid-stem cell exosome eye drops provided by the invention can effectively treat xerophthalmia, and are stable in components, safe and reliable. The three components including the tannic acid modified carboxymethyl chitosan, the hyaluronic acid and the adipose tissue-derived stromal cell exosome coexist, so that the functions of all the components can be fully played, a synergistic effect is achieved, and a better treatment effect on xerophthalmia is achieved.

The invention relates to the technical field of biology, and discloses a stem cell exosome anti-hair loss and hair growth complex and a preparation method thereof.A hair growth agent is wrapped and loaded in adipose-derived mesenchymal stem cell exosome to form a drug-loaded stem cell exosome; the medicine-carrying stem cell exosome is loaded in cross-linked hydrogel composed of a gentisic acid-cyclodextrin complex and epsilon-polylysine modified hyaluronic acid to form the stem cell exosome anti-hair loss and hair growth complex, and the stem cell exosome anti-hair loss and hair growth complex is directly smeared and externally applied to the scalp surface and acts on the hair follicle infection part in a targeted mode, so that the hair loss prevention and hair growth promotion effect is achieved. The stem cell exosome anti-hair-loss and hair-growth-promoting compound can inhibit bacterial growth, relieve and treat hair follicle inflammation caused by bacterial infection and improve the scalp environment, so that under the condition that inflammation is effectively controlled, the drug-loaded stem cell exosome in the stem cell exosome anti-hair-loss and hair-growth-promoting compound is stably released, damaged hair follicle cells are repaired, hair regeneration is promoted, the exosome treatment effect is prolonged, and the anti-hair-loss and hair-growth-promoting effect is achieved. The purpose of treating alopecia is achieved.

The invention discloses a Pickering emulsion with cinnamyl aldehyde as an oil phase and a preparation method of the Pickering emulsion. The Pickering emulsion is prepared by the following steps: carrying out a cross-linking reaction on anions released by sodium tripolyphosphate and protonated cations of chitosan to prepare chitosan nanoparticles as a water phase, and then taking cinnamyl aldehyde as an oil phase. The Pickering emulsion delivery system disclosed by the invention can effectively release cinnamyl aldehyde, shows the remarkable antibacterial property of cinnamyl aldehyde on a wound surface, promotes wound surface healing, and shows good treatment potential.

The invention relates to the field of sponge materials, and provides an antibacterial degradable sponge material and a preparation method thereof. The material is prepared from protocatechuic acid modified quaternized chitosan, quaternary ammonium salt grafted gelatin, sodium alginate, polyhydroxybutyrate, polycaprolactone, a polylactic acid-glycolic acid copolymer, hexamethylene diisocyanate, a foaming agent, nano cellulose, vitamin E, polyethylene glycol, triethylamine and dimethylformamide. Protocatechuic acid and quaternized chitosan are subjected to amidation modification, and chitosan and gelatin are subjected to quaternization treatment by adopting 2, 3-epoxypropyl trimethyl ammonium chloride, so that the antibacterial property and biodegradability of the material are improved. In the material preparation process, through vacuum degassing, foaming vacuum degree and humidity control and a multi-step drying process, it is ensured that the average pore size of the material is uniform (50-200 microns) and the porosity is high (50%-90%), and the obtained material has excellent antibacterial property and degradability and has wide application value.

The invention relates to the technical field of preparation of antibacterial gloves, in particular to a preparation method of degradable breathable antibacterial gloves, and an emulsion formula of the degradable breathable antibacterial gloves comprises the following components in parts by weight: 100 parts of nitrile rubber modified polylactic acid, 5-10 parts of modified antibacterial filler, 1-3 parts of an anti-aging agent, 0.5-3 parts of a vulcanizing agent, 0.3-2 parts of a vulcanization accelerator and 0.5-1 part of a foaming agent. Through a high-molecular compound composition system, nitrile rubber modified polylactic acid, modified antibacterial filler and other components are compounded to prepare the glove with controllable degradability, lasting antibacterial property and good air permeability; on the basis of covalent binding of silicon dioxide loaded with antibacterial groups and modified chitosan, the synergistic antibacterial effect of the modified antibacterial filler is achieved, and therefore the antibacterial performance of the glove is improved.

The invention discloses application of a low-toxicity broad-spectrum antibacterial peptide taking WW as a symmetric center, WK is repeated twice and symmetrically distributed on two sides, WW is taken as the symmetric center to obtain an antibacterial peptide WKWKWWKWKW-NH2 (N2W2), and tryptophan at the C terminal and the N terminal of the N2W2 is replaced with leucine and / or isoleucine to obtain the antibacterial peptide. The antibacterial peptide can basically maintain the excellent antibacterial activity of the parent peptide, and the hemolytic activity of the antibacterial peptide is greatly reduced, so that the safety is improved. Wherein the antibacterial activity of IL-N2W2 is the highest, but the stability of IL-N2W2 needs to be improved, so that D-IL-N2W2 is obtained by carrying out D-type amino acid substitution on IL-N2W2. The D-IL-N2W2 keeps the high antibacterial activity and low toxicity of IL-N2W2, can still completely inhibit the growth of bacteria after being incubated with high-concentration trypsin or chymotrypsin, has great potential in the aspect of relieving the drug resistance of antibiotics, and has good application prospects in preparation of clinical antibacterial drugs.

The invention discloses a destabilizing enzyme fusion protein with improved activity, and the amino acid sequence of the destabilizing enzyme fusion protein is as shown in SEQ ID No.3 or SEQ ID No.5. According to the destabilizing enzyme fusion protein disclosed by the invention, the bacteriostatic activity, thrombolytic activity and isopeptidase activity of the destabilizing enzyme fusion protein are improved by optimizing the expression strategy and modifying the sequence of destabilizing enzyme Wpdestalase from whitmania pigra; and the application prospect and the application space of the Wpestabase are improved.

The invention belongs to the technical field of materials, and discloses mildew-proof modified starch-based super absorbent resin as well as a preparation method and application thereof. The mildew-proof modified starch-based super absorbent resin is prepared from the following raw materials in parts by mass: 10 to 20 parts of modified carboxymethyl starch, 2 to 3 parts of quaternized carboxymethyl chitosan, 10 to 15 parts of 2-acrylamido-2-methylpropanesulfonic acid, 15 to 25 parts of acrylamide, 100 parts of acrylic acid, 0.4 to 0.7 part of a redox initiator and 0.05 to 0.15 part of a cross-linking agent. According to the invention, the inherent defects of low mechanical strength, poor salt tolerance, poor stability, easy decomposition, short service life and the like of the starch-based resin are obviously improved, so that the starch-based super absorbent resin with good mildew resistance and more excellent performance can be developed.

The invention belongs to the technical field of biological fermentation, and relates to a high-bacteriostatic-activity propionibacterium scherichia subspecies and application thereof, in particular to a propionibacterium scherichia subspecies which is classified and named as Propionibacterium fredenreichiii subsp. Scherichia CJPF0001, and the preservation number of the propionibacterium scherichia subspecies is CCTCC (China Center for Type Culture Collection) No.M 20242477. The invention further relates to a preparation method of the propionibacterium scherichia subspecies and application of the propionibacterium scherichia subspecies. The fermentation product of the strain has high protein and high bacteriostatic activity, can inhibit the growth of common spoilage microorganisms, and has the effects of improving the nutritional value of food and prolonging the shelf life of food and the like.

The invention provides a Trichoderma asperellum TAZ19, a biocontrol inoculant and application of the Trichoderma asperellum TAZ19, the Trichoderma asperellum TAZ19 is preserved in the China Center for Type Culture Collection on October 25, 2024, and the preservation number of the Trichoderma asperellum TAZ19 is CCTCC M 20242341. The active ingredients of the biocontrol inoculant comprise the trichoderma asperellum TAZ19 strain, and the trichoderma asperellum TAZ19 strain is one or more of conidia, hypha, fermentation filtrate or active extracts of the trichoderma asperellum TAZ19 strain of the trichoderma asperellum TAZ19 strain. The trichoderma asperellum TAZ19 has an excellent antagonistic effect on a plurality of plant disease pathogenic bacteria such as dispora pinnatifida, a plurality of fusarium oxysporum, apple black beancurd skin fungi and botryosphaeria dothidea, has broad-spectrum antibacterial activity, is high in growth speed, high in antibacterial activity and high in hyperparasitism ability, has better saline-alkaline tolerance, also has a growth promoting effect on plants, and can be widely applied to the field of plant diseases. The strain can be used as an active component of a biocontrol inoculant to be widely applied to prevention and treatment of various plant diseases such as pine tree branch rot, wheat stem rot and tomato fusarium wilt, and has a wide application prospect.

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention belongs to the field of biomedical materials, and discloses an antibacterial bone cement modified by methacrylic acid 3, 4-dichloro-5-hydroxy-5H-furan-2-ketone ester and a preparation method of the antibacterial bone cement modified by methacrylic acid 3, 4-dichloro-5-hydroxy-5H-furan-2-ketone ester. According to the invention, 3, 4-dichloro-5-hydroxy-5H-furan-2-ketone (DHF) is covalently connected with methacrylic acid (MAA) to prepare a novel monomer, namely methacrylic acid 3, 4-dichloro-5-hydroxy-5H-furan-2-ketone ester (DHF-MAA), which is an oily liquid easily dissolved in methyl methacrylate (MMA). The obtained DHF-MAA is used for liquid phase modification of bone cement, and the DHF-MAA modified antibacterial bone cement is finally prepared and is novel NLBC. The DHF-MAA modified bone cement is excellent in antibacterial activity, mechanical strength and biocompatibility, and the excellent performance of the DHF-MAA modified bone cement shows that the DHF-MAA modified bone cement has a wide prospect in clinical application.

The invention discloses Burkholderia gladioli HM-72 and application thereof, the Burkholderia gladioli HM-72 is preserved in China Center for Type Culture Collection on July 13, 2023, the preservation address is Wuhan University, Wuhan, No. 299, eight road, Wuchang District, Wuhan, Hubei, and the preservation number is CCTCC NO: M20231291. The strain is high in Pb stress resistance, and has the capability of partially dissolving phosphorus and producing siderophores. After the strain is applied to the Pb-polluted soil, the migration rate of Pb can be improved, the removal of Pb in the soil is accelerated, the Pb is accumulated in the overground part of the enrichment plant, the problem of repairing the lead pollution of the soil is solved, meanwhile, the strain can inhibit the growth of pathogenic fungi, the growth promoting and bacteriostasis characteristics of the strain can provide favorable conditions for the growth of the enrichment plant in a high-lead environment, and the application prospect is wide. The biomass accumulation is promoted, and the method has an application prospect in heavy metal polluted environment restoration.

The invention discloses a quaternized chitosan benzisothiazolinone derivative as well as a preparation method and application of the quaternized chitosan benzisothiazolinone derivative in efficient antibacterial performance. The derivative has a structure as shown in a formula 1. The preparation method comprises the following steps: reacting chitosan with glycidyl trimethyl ammonium chloride at 60-100 DEG C for 6-48 hours to obtain a product 1, namely hydroxypropyl trimethyl ammonium chloride chitosan (HACC); the preparation method comprises the following steps: reacting 1, 2-benzisothiazolin-3-one with sodium chloroacetate at the temperature of 110-140 DEG C for 12-36 hours to obtain a product 2: 2-(3-carbonyl benzo [d] isothiazole-2-(3H)-yl) acetic acid (BITCOOH); and reacting the product 1 with the product 2 under a neutral condition for 12-48 hours to obtain the quaternized chitosan benzisothiazolinone derivative (HACCBIT). The derivative has a broad-spectrum antibacterial property, and has a killing effect on a plurality of bacteria, such as staphylococcus aureus, escherichia coli, salmonella salmonella salmonella, lactobacillus paracasei, lysinibacillus sphaericus, acinetobacter lwoffii and the like, and the derivative can be used for killing a plurality of bacteria, such as the staphylococcus aureus, the escherichia coli, the salmonella salmonella salmonella, the lactobacillus paracasei, the lysinibacillus sphaericus, the acinetobacter lwoffii and the like.

The invention discloses a preparation method and application of photo-thermal antibacterial hydrogel for MRSA (Methicillin Resistant Staphylococcus Aureus) infected diabetes wounds, and relates to the technical field of biological tissue engineering. The method comprises the following steps: uniformly dispersing ZIF-8 (at) PDA nanoparticles in a carboxymethyl chitosan solution, adding a gelatin solution, uniformly mixing, adding into an oxidized sodium alginate solution, and carrying out a Schiff base reaction to obtain the photo-thermal antibacterial hydrogel for MRSA infected diabetes wounds. The hydrogel disclosed by the invention is prepared by crosslinking carboxymethyl chitosan, gelatin, oxidized sodium alginate and dopamine modified ZIF-8 nanoparticles through a Schiff base bond. Carboxymethyl chitosan (CMCS) has excellent biocompatibility, antibacterial activity and adjustable and controllable mechanical properties, and can effectively preserve moisture, form a breathable and moist environment and promote skin healing.

The invention belongs to the technical field of microorganisms, and discloses an actinomycete, which is streptomyces aureoflavus CYS4-5, is preserved in China General Microbiological Culture Collection Center (CGMCC), is preserved on October 17, 2023, and has the preservation number of CGMCC No.28506. The actinomycete has the advantages that the actinomycete can be used for preparing an actinomycete culture medium; the strain has the potential of preventing and treating salvia miltiorrhiza root rot, has obvious bacteriostatic activity on gaeumannomyces graminis, rhizoctonia cerealis, exserohilum turcicum, sclerotinia sclerotiorum, wheat root rot, fusarium graminearum and botryosphaeria dothidea, and plays an important role in biological prevention and treatment of diseases of crops such as wheat, corn, rape and grapes.

The invention discloses a preparation method and application of chlorine dioxide preservative paper.The preparation method comprises the steps that starch, nanocellulose, hydrogen peroxide, ferric sulfate, distilled water and sodium hydroxide are mixed to obtain gelatinized starch, and then the gelatinized starch is mixed with water-based acrylic resin, casein, alkyl glycoside and ammonium persulfate to obtain composite starch glue; mixing sodium chlorite and composite starch glue to obtain fresh-keeping coating liquid A, and then coating the fresh-keeping coating liquid A on fresh-keeping base paper to obtain fresh-keeping coating liquid A paper; citric acid and distilled water are mixed to obtain fresh-keeping coating liquid B, and then fresh-keeping base paper is soaked in the fresh-keeping coating liquid B to obtain fresh-keeping coating liquid B paper; the fresh-keeping coating liquid A paper and the fresh-keeping coating liquid B paper are attached to obtain the chlorine dioxide fresh-keeping paper. The chlorine dioxide fresh-keeping paste is pasted on the inner side of the blueberry packaging box cover to keep the blueberries fresh. The chlorine dioxide preservative paper prepared by the invention can solve the problems of burst release and short retention time of chlorine dioxide, can reduce the problems of easy softening, rot and deterioration of picked blueberries, prolongs the shelf life of the blueberries, and improves the commodity value of the blueberries.

The invention discloses a preparation method of a phenolic chitosan quaternary ammonium salt antibacterial agent, which comprises the following steps: alkalizing chitosan at low temperature, then carrying out substitution reaction on the alkalized chitosan and a carboxylic acid derivative, and introducing a reactive carboxyl group on the hydroxyl of the chitosan to obtain O-carboxylated chitosan, so as to provide reactive sites for subsequent secondary modification; the preparation method comprises the following steps: preparing O-carboxylated chitosan, introducing a quaternary ammonium salt group on an amino group of the O-carboxylated chitosan through a ring-opening reaction so as to improve the antibacterial property and water solubility of the chitosan, and finally reacting with a phenolic derivative to obtain the phenolic chitosan quaternary ammonium salt antibacterial agent. According to the preparation method of the phenolic chitosan quaternary ammonium salt antibacterial agent, industrial production is easy, and the prepared phenolic chitosan quaternary ammonium salt antibacterial agent has good antibacterial performance and free radical scavenging capacity and also has good water solubility.

The invention belongs to the technical field of probiotics, and particularly relates to pediococcus acidilactici with acid resistance, bile salt resistance and antibacterial activity and application of the pediococcus acidilactici. The pediococcus acidilactici TR19 has the preservation number of CGMCC NO.31330 and is classified and named as pediococcus acidilactici according to microorganisms. The strain has good acid resistance and bile salt resistance, and can be used for preparing probiotics for preventing and treating bacteria. Meanwhile, the strain has a relatively strong bacteriostatic effect on escherichia coli, bacillus cereus, staphylococcus aureus and salmonella typhimurium, in addition, the strain has a relatively good degradation capability on nitrite and cholesterol, and the applicability is relatively strong.

The invention discloses a phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative as well as preparation and antibacterial application thereof. According to the series of compounds, phenanthro [9, 10-d] imidazole quaternary ammonium salt derivatives which are novel in structure and good in antibacterial activity are prepared by taking phenanthro quinone as a lead compound and modifying the structure of phenanthro quinone, the structural general formula of the phenanthro [9, 10-d] imidazole quaternary ammonium salt derivatives is shown in the specification, and R is selected from H, C6-C14 alkyl or C3-C8 alkylene. The phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative prepared by the invention has a relatively good antibacterial effect on gram-positive bacteria such as staphylococcus aureus S.aureus ATCC 29213 and various clinical methicillin-resistant staphylococcus aureus (MRSA), meanwhile, the water solubility is improved, the biotoxicity of a parent body is reduced, and the phenanthro [9, 10-d] imidazole quaternary ammonium salt derivative has relatively high yield and is suitable for industrial production. The compound is expected to be further developed into a clinical candidate antibacterial drug. # imgabs0 #

The invention provides a preparation method and application of a biochar-based microbial inoculant, the biochar-based microbial inoculant is prepared from bacillus megatherium L2 with the preservation number of CCTCC NO: M2012381 and rice hull biochar, and the preparation method of the biochar-based microbial inoculant comprises the steps of bacillus megatherium L2 seed liquid culture and liquid fermentation, biochar proportion mixing adsorption, standing and drying. The biochar-based microbial agent prepared by the invention has a good effect of preventing and treating the tea tree anthracnose, effectively reduces the infection ability of tea tree anthracnose related pathogenic bacteria, can also effectively recruit beneficial microorganisms such as chloromentholum, actinomycelum and thick-wall mycolum in soil, further improves the prevention and treatment effect on the tea tree pathogenic bacteria, and has a good application prospect in the field of prevention and treatment of tea tree anthracnose. The method has huge application potential in protection and prevention of the tea trees and is beneficial to good growth of the tea trees.

The preparation method comprises the following steps: firstly, introducing a quaternary ammonium salt group to an amino group of chitosan to improve the antibacterial property and water solubility of the chitosan, then further introducing a pyridine group to provide a basis for introducing a phenolic group and biquaternary ammonium salt, and finally, carrying out quaternization reaction to obtain the antibacterial and antioxidant phenolic chitosan biquaternary ammonium salt. Quaternary ammonium salt is introduced for the second time, and the phenol-based chitosan biquaternary ammonium salt is obtained. The preparation method of the antibacterial and antioxidant phenolic chitosan biquaternary ammonium salt is simple and convenient to operate and wide in raw material source, and the prepared phenolic chitosan biquaternary ammonium salt is soluble in water and has good antibacterial and antioxidant properties.

The invention relates to the technical field of microorganisms, in particular to a Streptomyces asenjonii R02 strain and application thereof.The strain R02 is a microorganism screened by an applicant, and it is detected that the strain has good growth promoting and disease resisting effects, specifically, the strain can produce siderophores, secrete cellulase and degrade cellulose; meanwhile, a bacteriostatic activity test proves that the bacterium has good bacteriostatic activity on gram-negative bacillus, and particularly has a good bacteriostatic effect on ralstonia solanacearum; pot experiments and field experiments prove that the microorganism has a remarkable disease-resistant effect on plant soil-borne bacterial wilt caused by Ralstonia solanacearum, has a good growth-promoting effect on plants, especially ginger, and can be used for preparing biological bacterial fertilizer to effectively prevent and control the soil-borne bacterial wilt.

The invention provides bacillus atrophaeus LYZ1127, a biocontrol microbial inoculum and application of the biocontrol microbial inoculum. The preservation number of the bacillus atrophaeus LYZ1127 is CGMCC (China General Microbiological Culture Collection Center) No.31718, the preservation number of the bacillus atrophaeus LYZ1127 is CGMCC No.31718, and the preservation number of the biocontrol microbial inoculum is CGMCC No.31718. The bacillus atrophaeus LYZ1127 has relatively good bacteriostatic activity on 11 pathogenic bacteria of six common diseases of alfalfa and verticillium dahliae (separated from oxytropis aureocauda plants) belonging to the same genus and different species with verticillium wilt of alfalfa, and pot experiment shows that the bacillus atrophaeus LYZ1127 can promote the growth of alfalfa root systems and plants, can also remarkably reduce the incidence rate of verticillium wilt of alfalfa, and can be applied to the field of agricultural chemical engineering, such as agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering, agricultural chemical engineering and agricultural chemical engineering. The method has a good control effect on the verticillium wilt of the alfalfa, and provides an important theoretical basis for biological control of the verticillium wilt of the alfalfa.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide cAMP-1116nc with a broad-spectrum antibacterial effect and application of the antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc is obtained by carrying out N-terminal acetylation modification and C-terminal amidation modification on a polypeptide as shown in SEQ ID NO. 1. The antibacterial peptide cAMP-1116nc is an antibacterial peptide cAMP-1116nc. The antibacterial peptide cAMP-1116nc disclosed by the invention shows remarkable antibacterial activity in a plurality of gram-positive bacteria and gram-negative bacteria. The antibacterial peptide cAMP-1116nc can efficiently kill clinically common gram-negative pathogenic bacteria acinetobacter baumannii under the conditions that the pH is equal to 2, the pH is equal to 7.4 and the pH is equal to 8.4, has relatively low cytotoxicity, has the potential of preparing antibacterial drugs, and is suitable for developing antibacterial products with multiple purposes, including the antibacterial drugs, disinfectants and biological preservatives. The invention provides a novel functional polypeptide for the field of antibacterial peptides, and the novel functional polypeptide has important practical application value.